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Cell Biochem Biophys ; 76(1-2): 243-253, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-28726179

RESUMO

In this study we investigated the effect of acute and chronic treatment with Met and/or methionine sulfoxide (MetO) on ectonucleotidases and cholinesterases activities from lymphocytes and purine derivatives compounds, C-protein reactive, interleukin-10, interleukin-6, and tumor necrosis factor-α levels in serum of young rats. Adenosine triphosphate hydrolysis was decreased in lymphocytes 1 h after treatment by MetO and Met + MetO. However, adenosine triphosphate and adenosine diphosphate hydrolysis in lymphocytes was increased in the groups MetO and Met + MetO and adenosine deaminase activity was increased in MetO 3 h after the treatment. Acetylcholinesterase activity was increased in lymphocytes after 3 h and 21 days of treatment by MetO and Met + MetO, while serum butyrycholinesterase activity was decreased after 1 h and 21 days of treatment in the same groups. In chronic treatment, interleukin-6 and tumor necrosis factor-α level were increased, while that interleukin-10 level was decreased by Met, MetO, and Met + MetO when compared to control group. C-protein reactive level was increased by MetO and Met + MetO. Adenosine triphosphate and adenosine monophosphate levels were reduced in all amino acids treated groups, while adenosine diphosphate and hypoxanthine were enhanced by MetO and Met + MetO. Adenosine and xanthine were reduced in the MetO group, whereas inosine levels were decreased in the MetO and Met + MetO groups. These findings help to understand the inflammatory alterations observed in hypermethioninemia.


Assuntos
Ativação Enzimática/efeitos dos fármacos , Metionina/análogos & derivados , Metionina/farmacologia , Acetilcolinesterase/metabolismo , Nucleotídeos de Adenina/metabolismo , Adenosina Desaminase/metabolismo , Envelhecimento , Animais , Proteína C-Reativa/análise , Células Cultivadas , Interleucina-10/sangue , Interleucina-6/sangue , Linfócitos/citologia , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa/sangue
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