Nutritional status among earthquake survivors: a systematic review and meta-analysis.

OBJECTIVE: Earthquakes, as one of the most devastating natural disasters, have many consequences, including the collapse of buildings where food is produced, stored, and distributed, which can ensue with numerous nutritional problems. This study was to investigate the nutritional status of earthquake survivors. STUDY DESIGN: This was a systematic review and meta-analysis study. METHODS: The present review was conducted according to the PRISMA guideline. The data were collected by searching the data resources of PubMed, Scopus, Web of Science, Science Direct, Google Scholar, MagIran, and Scientific Information Database. Meta-analysis was conducted using the random effects model, and the I2 index was used to assess heterogeneity among studies. Publication bias was assessed using Begg's test. RESULTS: In this study, 342 studies were identified in the primary literature search, and after removing duplicates, 14 of which were finally selected for meta-analysis. Based on the results of the meta-analysis, the overall prevalence was obtained: 4.19% for wasting, 16.78% for stunting, 12.59% for underweight, and 28.06% for anemia after the earthquake. CONCLUSION: The results indicated that earthquake survivors are exposed to malnutrition. Therefore, it is recommended that adequate food and nutritional supplements are provided to all earthquake survivors.

Biotyping and enterotoxigenicity of coagulase-positive and coagulase-negative staphylococci isolated from different raw meat.

BACKGROUND: Staphylococcal enterotoxins (SEs) are produced in foods under favorable conditions and considered a potential biological threat. AIMS: The study was performed to detect enterotoxigenic genes of A and B in coagulase-positive (CPS) and coagulase-negative staphylococci (CNS) to evaluate biotypes and antibiotic resistance of isolated Staphylococcus aureus from different meat. METHODS: A total of 160 meat swab samples were collected from lamb, water buffalo, cattle, and chicken carcasses. Presumptive colonies on Baird Parker agar were subjected to biochemical identification, including Gram staining, catalase, oxidase, and coagulase activity. Relevant colonies separately were subjected to the polymerase chain reaction (PCR) assay for identification of Staphylococcus genus, enterotoxigenic genes (sea and seb) and the thermonuclease gene (nuc) specific for the S. aureus. The antibiotic susceptibility test was also carried out using five antibiotics. RESULTS: Totally, 150 Staphylococcus spp. were isolated from the samples among which 135 (90%) isolates harbored sea gene, meanwhile, none of the isolates contained seb gene. Twenty-five S. aureus confirmed by PCR from which 15 isolates (60%) belonged to host specific (HS), 7 isolates (28%) belonged to non-host specific (NHS) biotypes, while 3 isolates (12%) were non-typable. Overall, 68%, 56%, 16%, 12%, and 8% of isolates were resistant to penicillin, trimethoprim, gentamicin, oxacillin, and erythromycin, respectively. CONCLUSION: The meats provided from this area were contaminated with enterotoxigenic and antibiotic-resistance staphylococci, which can threaten the health of the consumers. The study showed that not only CPS contain enterotoxin A gene, but also CNS isolates possess this gene, especially in buffalo meat, and thus they are recognized as potential hazards in different meats.

The Relationship Between Vitamin D and Telomere/Telomerase: A Comprehensive Review.

Telomeres are repetitive nucleotide sequences that together with the associated sheltrin complex protect the ends of chromosomes and maintain genomic stability. Evidences from various organisms suggests that several factors influence telomere length regulation, such as telomere binding proteins, telomere capping proteins, telomerase, and DNA replication enzymes. Recent studies suggest that micronutrients, such as vitamin D, folate and vitamin B12, are involved in telomere biology and cellular aging. In particular, vitamin D is important for a range of vital cellular processes including cellular differentiation, proliferation and apoptosis. As a result of the multiple functions of vitamin D it has been speculated that vitamin D might play a role in telomere biology and genomic stability. In this study, our main goal is investigating the relationship between telomerase enzyme and vitamin D. Findings of this study suggest that higher vitamin D concentrations, which are easily modifiable through nutritional supplementation, are associated with longer LTL, which underscores the potentially beneficial effects of this hormone on aging and age-related diseases. Vitamin D may reduce telomere shortening through anti-inflammatory and anti-cell proliferation mechanisms. Significant Low levels of telomerase activity create short telomeres, which in turn signal exit from the cell cycle resulting in cell senescence and apoptosis. In follow-up examination, the patients who remained vitamin D deficient tended to have shorter telomeres than those patients whose 25-hydroxyvitamin D levels were depleted. Increasing 25-hydroxyvitamin D levels in patients with SLE may be beneficial in maintaining telomere length and preventing cellular aging. Moreover, anti-telomere antibody levels may be a promising biomarker of SLE status and disease activity.

Early diagenetic behavior of arsenic in the sediment of the hypersaline Maharlu Lake, southern Iran.

Arsenic (As) is a toxic element can experience phase and speciation changes during the early diagenesis of sediments across the sediment-water interface, affecting its mobility and toxicity. Maharlu Lake is a hypersaline lake in Southern Iran that today receives significant inflow from urban, industrial and agricultural wastewater. A 1-m core was sampled from the lake sediment in an area far away from the major inflows. We performed a semi-quantitative analysis of the elemental composition, and a quantitative analysis of the total As concentration, pH, loss on ignition, water content, salt content, and grain size distribution to characterize changes in sediment composition with depth and their influence on the variability of As partitioning and speciation. Nine characterized lithologies were selected for a sequential As extraction analysis to study the vertical variations in phase and speciation of As in the heterogenic lithologies. Total As concentrations range from 1.0 µg g-1 to 13.6 µg g-1. As is present mostly in HNO3 and HCl extractable fractions with As5+ being the predominant species. As3+ was mostly found in phosphate and NH2OH·HCl extractable fractions. The concentration of mobile As fractions generally decrease with depth. It seems that the As stored in volatile sulfides and very poorly crystalline Fe and Al hydr-oxides in the shallow sediment transforms to sulfides phases during burring. Our findings show that the mobility and toxicity of As decreases during early diagenetic processes in Maharlu lake. However, the As can be remobilized either via changes in the redox conditions in the lake.

Short-term heat shock proteins 70 and 90 mRNA expression profile and its relation to thermo-physiological parameters in goats exposed to heat stress.

To follow the inheritance potential for heat tolerance after a crossing program in goats, 24 kids from four groups of goat kids (6 kids in each) from Aradi (A) and Damascus (D), their crossbred first-generation F1 (½D½A) and second-generation F2 (¾D»A), were exposed to acute elevated environmental temperature in controlled climatic chambers. Rectal temperature (RT), respiratory rate (RR), heat tolerance coefficient (HTC), adaptability coefficient (AC), and mRNA expression of heat shock proteins 70 and 90 ((HSP70 and HASP90, respectively), using real-time PCR were estimated. Results showed that Aradi breed goats had the highest level of expression for heat shock proteins 70 and 90, followed by F1, F2, and Damascus (P ≤ 0.01). Crossbreeds and Damascus showed the highest RT, while Aradi breed showed the lowest value (P ≤ 0.01). Aradi and crossbreeds showed the highest RR, while Damascus showed the lowest RR (P ≤ 0.05). Aradi and F1 showed the highest HTC (P ≤ 0.05), while there was no significant difference between pure breeds and crossbreeds in AC. A significant positive phenotypic correlation (0.81) was observed between HSP70 and HSP90. In addition, RR showed moderate positive correlation with both HSP70 and HSP90. It could be concluded that Aradi breed had putative heat tolerance in comparison to its crossbred progeny from the Damascus breed. The crossbreeding may result in some loss of heat tolerance potential, but the crossbreeds still better adapted to high environmental temperature than the Damascus breed.

Effect of sequential treatments with sodium dodecyl sulfate and citric acid or hydrogen peroxide on the reduction of some foodborne pathogens on eggshell.

The aim of this study was to investigate the effect of sodium dodecyl sulfate (SDS), citric acid, and hydrogen peroxide (H2O2), alone or in combination, on reducing the population of four foodborne pathogens, including Escherichia coli, Listeria monocytogenes, Salmonellatyphimurium, and Staphylococcus aureus on eggshells. In each series of tests, eight fresh eggs were inoculated with each bacterial strain by being immersed in a bacterial suspension and exposed to SDS (1.5%), H2O2 (0.5%), citric acid (1%), or sequential treatments with SDS + citric acid and SDS + H2O2. Viable cell counts were made and the bacterial concentrations results compared to pre-treatment levels. Results showed that all washing solutions except citric acid significantly (P<0.05) reduced the concentration of all tested bacteria (~2-4 log reductions). The sensitivity of S. typhimurium and E. coli to SDS and H2O2 was similar (~2.5 log reduction). Listeria monocytogenes (4.1 Log reduction) and S. aureus (4.3 Log reduction) were more sensitive to SDS and H2O2, respectively. The combination of SDS and citric acid or H2O2 in comparison to SDS alone, generally did not produce significant additive reductions in the viability of the bacteria on eggshells. These data suggest that SDS potentially could be used alone or in combination with citric acid or H2O2 as an effective and inexpensive method to reduce bacteria, such as L. monocytogenes, on eggshells. Additionally, application of SDS may be useful for bacterial decontamination of other materials and surfaces in food industries.

Effects of testosterone enanthate treatment in conjunction with resistance training on thyroid hormones and lipid profile in male Wistar rats.

This study was conducted to determine the effects of 8-week administration of testosterone enanthate (TE) in conjunction with resistance training on thyroid hormones and lipid profiles. Sixty male adult Wistar rats were randomly divided into six groups: C: olive oil, RT: resistance training + olive oil, LD: TE (20 mg/kg), HD: TE (50 mg/kg), RT + LD: RT + TE (20 mg/kg), RT + HD: RT + TE (50 mg/kg). The RT consisted of climbing (5 reps/3 sets) a ladder carrying a load suspended from the tail. At the end, blood specimens were obtained from the orbital sinus and serum concentration of T4, T3, TSH and lipid profiles was determined. The serum concentration of TSH significantly increased in RT + HD group compared to C, and the serum concentration of T4 significantly decreased in LD, HD, RT + LD and RT + HD groups compared to the C and RT groups (p < .05). The concentration of HDL and cholesterol significantly decreased in HD and RT + HD groups compared with C group (p < .05). Both decreased T4 and increased TSH in the RT + HD group likely suggested a primary hypothyroidism as a complication of high-dose administration of testosterone enanthate along with resistance training. Alteration in lipid profile was another complication observed in rats received high doses of testosterone enanthate.

Synergistic Effects of NDRG2 Overexpression and Radiotherapy on Cell Death of Human Prostate LNCaP Cells.

BACKGROUND: Radiation therapy is among the most conventional cancer therapeutic modalities with effective local tumor control. However, due to the development of radio-resistance, tumor recurrence and metastasis often occur following radiation therapy. In recent years, combination of radiotherapy and gene therapy has been suggested to overcome this problem. The aim of the current study was to explore the potential synergistic effects of N-Myc Downstream-Regulated Gene 2 (NDRG2) overexpression, a newly identified candidate tumor suppressor gene, with radiotherapy against proliferation of prostate LNCaP cell line. MATERIALS AND METHODS: In this study, LNCaP cells were exposed to X-ray radiation in the presence or absence of NDRG2 overexpression using plasmid PSES- pAdenoVator-PSA-NDRG2-IRES-GFP. The effects of NDRG2 overexpression, X-ray radiation or combination of both on the cell proliferation and apoptosis of LNCaP cells were then analyzed using MTT assay and flow cytometery, respectively. RESULTS: Results of MTT assay showed that NDRG2 overexpression and X-ray radiation had a synergistic effect against proliferation of LNCaP cells. Moreover, NDRG2 overexpression increased apoptotic effect of X-ray radiation in LNCaP cells synergistically. CONCLUSION: Our findings suggested that NDRG2 overexpression in combination with radiotherapy may be an effective therapeutic option against prostate cancer.

Vitamin D increases IGF-I and insulin levels in experimental diabetic rats.

INTRODUCTION AND OBJECTIVE: Previous studies have found that IGF-I may play an important role in glucose metabolism. The aim of this study is to examine the effect of vitamin D intake on the serum levels of glucose, insulin, and IGF-I in experimental diabetic rats. MATERIAL AND METHODS: A total of 24 male Sprague-Dawley rats aged six to seven months, with an average weight of 300±30g, were randomly divided into three groups (eight rats per group). The first group served as control and the other two groups received an intraperitoneal injection of 45mg/kg streptozotocin (STZ) to develop diabetes. Then groups were treated for four weeks either with placebo or vitamin D (two injections of 20,000IU/kg). RESULTS: At the end of the experiment, two injection of vitamin D were found to result in a significant increase in plasma cholecalciferol, which could improve hyperglycaemia and hypoinsulinemia in diabetic rats. HbA1c concentration had a slight and insignificant decrease following vitamin D intake. In addition, a significant decline was observed in the serum IGF-I level of STZ-treated rats in comparison to the controls, which was compensated in the vitamin D group. The serum vitamin D concentration was positively correlated to the changes in IGF-I level by Pearson test. CONCLUSIONS: These data showed for the first time that vitamin D intake could significantly improve fasting plasma glucose, insulin, and IGF-I in an experimental type 1 diabetes model.

Effects of L-carnitine supplementation on biomarkers of oxidative stress, antioxidant capacity and lipid profile, in patients with pemphigus vulgaris: a randomized, double-blind, placebo-controlled trial.

BACKGROUND/OBJECTIVES: Pemphigus vulgaris (PV), as an autoimmune disease including mucosa and the skin, is associated with several complications and comorbidities. The present study planned to determine the effect of L-carnitine (LC) supplementation on biomarkers of oxidative stress (OS), antioxidant capacity and lipid profile in PV patients.Subjects/MethodsFifty two control and patients with PV, participated in the current randomized, double-blind, placebo-controlled clinical trial. The patients were allocated randomly to receive 2 g per day LC tartrate subdivided into two equal doses of 1 g before breakfast and dinner (n=26) or placebo (n=26) for 8 weeks. Anthropometric, lipid profile and OS values were determined at baseline and end of intervention period. RESULTS: LC intake significantly reduced serum levels of triglycerides, total-, LDL- cholesterol and oxidative stress index (OSI; P<0.05). In addition, supplementation with LC resulted to a meaningful increase in levels of total antioxidant capacity (TAC) (P=0.05) and serum carnitine (P<0.001). LC intake revealed non-significant change in serum total oxidant capacity (P=0.15) and HDL- cholesterol (P=0.06) in comparison to the placebo. CONCLUSIONS: LC consumption may have favorable results on TAC, OSI and lipid profiles in patients with PV. The results were in line with the idea that LC supplementation can be associated with positive effects on metabolic status and OS of patients with PV.European Journal of Clinical Nutrition advance online publication, 23 August 2017; doi:10.1038/ejcn.2017.131.

"Assessment of relationship between xerostomia and oral health-related quality of life in patients with rheumatoid arthritis".

OBJECTIVE: To determine the relationship between xerostomia and oral health-related quality of life in patients with rheumatoid arthritis. MATERIALS AND METHOD: Two hundred patients with rheumatoid arthritis were assessed using Fox and OHIP-14 questionnaires concerning xerostomia and oral health-related quality of life, respectively. The sum of decayed, missing, and filled teeth (DMFT) was determined via an intra-oral examination. In addition, intergroup comparisons were evaluated using t test, chi-square, regression, and Tukey analysis. RESULT: Among rheumatoid arthritis patients, 51% had been afflicted with xerostomia. We found a statistically significant relationship between xerostomia and oral health-related quality of life (p-value=.004), as xerostomia cases have significantly worse oral health-related quality of life. Also, there was a statistically significant association between oral health-related quality of life and gender as well as DMFT. CONCLUSION: Because there seem to be a high prevalence of xerostomia in patients with rheumatoid arthritis, screening in such population is highly recommended. Therefore, educational programs and/or workshops should be encouraged among healthcare providers to prevent worsening of oral health-related quality of life.

Evaluation of genetic diversity of Saudi native chicken populations using microsatellite markers.

The present study was conducted to evaluate the genetic diversity and relationships of 5 Saudi native chicken populations and a White Leghorn (L) strain as an exotic breed, using 25 microsatellite markers. According to morphological appearance and plumage color and pattern, they were classified into black (BL), black-barred (BR), dark brown (DB), light brown (LB), and gray (G) breeds. Twenty-five microsatellite markers were chosen based on the degree of polymorphism reported in the previous literature. The results revealed that all studied markers exhibited a varied percentage of polymorphism among native chicken populations. However, there were 81 polymorphic loci out of 109 loci. Based on cluster analysis, 3 main clusters of chicken populations were identified. Two clusters comprised native chicken breeds, while the L strain set the third one. Higher similarity was found between either black and gray populations (BL and G) or brown populations (LB and DB). They had a closer relationship to each other. Genetic distance between each couple was 0.11 and 0.12, respectively. The productive results revealed that the black breed had significantly (P < 0.0001) lighter body weight at sexual maturity compared to the other breeds. There was no significant difference between light and dark brown breeds for most productive traits. The genetic diversity we found among Saudi chicken populations may be of interest for improving productive performance and adaptability.

Abnormal frontostriatal connectivity in adolescent-onset schizophrenia and its relationship to cognitive functioning.

BACKGROUND: Adolescent-onset schizophrenia (AOS) is associated with cognitive impairment and poor clinical outcome. Cognitive dysfunction is hypothesised, in part, to reflect functional dysconnectivity between the frontal cortex and the striatum, although structural abnormalities consistent with this hypothesis have not yet been demonstrated in adolescence. OBJECTIVE: To characterise frontostriatal white matter (WM) tracts in relation to cognition in AOS. DESIGN: A MRI volumetric and diffusion tensor imaging study. PARTICIPANTS: Thirty-seven AOS subjects and 24 age and sex-matched healthy subjects. OUTCOME MEASURES: Using probabilistic tractography, cortical regions with the highest connection probability for each striatal voxel were determined, and correlated with IQ and specific cognitive functions after co-varying for age and sex. Fractional anisotropy (FA) from individual tracts was a secondary measure. RESULTS: Bayesian Structural Equation modeling of FA from 12 frontostriatal tracts showed processing speed to be an intermediary variable for cognition. AOS patients demonstrated generalised cognitive impairment with specific deficits in verbal learning and memory and in processing speed after correction for IQ. Dorsolateral prefrontal cortex connectivity with the striatum correlated positively with these measures and with IQ. DTI voxel-wise comparisons showed lower connectivity between striatum and the motor and lateral orbitofrontal cortices bilaterally, the left amygdalohippocampal complex, right anterior cingulate cortex, left medial orbitofrontal cortex and right dorsolateral prefrontal cortex. CONCLUSIONS: Frontostriatal dysconnectivity in large WM tracts that can explain core cognitive deficits are evident during adolescence. Processing speed, which is affected by alterations in WM connectivity, appears an intermediary variable in the cognitive deficits seen in schizophrenia.

Factors Governing the Impact of Emerged Salt Diapirs on Water Resources.

Salt diapirs in southern Iran are typically in contact with karstic and alluvial aquifers and consequently they are the most likely sources of groundwater salinization in this arid region. However, there are some salt diapirs that have no significant degradation effect on adjacent aquifers. Assessments of 62 of 122 Iranian-emerged salt diapirs based on geological, geomorphological, hydrogeological, and hydrochemical investigations indicated that 45% of the studied salt diapirs did not have a negative impact on surrounding water resources, whereas 55% of the salt diapirs have degraded water quality of adjacent aquifers. The impacts ranged from low- to high-grade salinization. We characterize here four major factors that control the impact of salt diapirs on surrounding water resources: (1) the evolutionary stage of the diapir, (2) the geological and (3) hydrogeological setting of the diapir, and (4) human activities. Identification of the major factors governing the influence of salt diapirs on the adjacent aquifers is necessary to understand the mechanism of salt diapir impact on adjacent aquifers, and subsequently to decide how to mitigate the deteriorating effect of the diapirs on the surrounding water resources.

The effect of vitamin K1 supplementation on sensitivity and insulin resistance via osteocalcin in prediabetic women: a double-blind randomized controlled clinical trial.

BACKGROUND/OBJECTIVES: A relationship between osteocalcin (OC) levels and factors associated with energy metabolism and insulin resistance has been reported recently. The aim of this study was to investigate whether modulation of ostecalcin isoforms via vitamin K1 supplementation would affect glucose metabolism or insulin sensitivity in prediabetic and premenopause women. SUBJECTS/METHODS: Eighty-two prediabetic women were randomized to consume vitamin K1 supplement (n = 39) or placebo (n = 43) for 4 weeks. Participants in the vitamin K1 supplement group received one pearl softgel capsule containing 1000 µm of phylloquinone, and the placebo group received one placebo capsule daily for 4 weeks. Blood samples were collected at baseline and after the 4-week intervention period to quantify carboxylated OC (cOC), undercarboxylated OC (ucOC) and relevant variables. RESULTS: Phylloquinone supplementation increased the serum levels of cOC and decreased ucOC, compared with placebo (12.53 ± 5.95 compared with 7.43 ± 4.85 ng/ml and 2.47 ± 1.91 compared with 4.79 ± 2.43 ng/ml, respectively; P < 0.001). Furthermore, intake of phylloquinone supplement led to significant decreases in %ucOC (17.97 ± 12.24 compared with 43.80 ± 19.86) and 2-h post-oral glucose tolerance test (OGTT) glucose (7.32 ± 1.50 compared with 8.62 ± 1.45 mmol/l), and 2-h post-OGTT insulin level (80.34 ± 42.24 compared with 112.43 ± 53.19 µIU/ml) and increased insulin sensitivity index (2.46 ± 0.71 compared with 1.75 ± 0.61) compared with placebo. Overall, a significant association was found between changes in %ucOC and changes in 2-h post-OGTT glucose (r = 0.308, P = 0.028). CONCLUSIONS: The results of this study demonstrated that vitamin K1 supplementation for 4 weeks did not affect insulin resistance in premenopausal and prediabetic women but had beneficial effects on glycemic status and insulin sensitivity.

Evaluation of antioxidant enzyme activity and antioxidant capacity in patients with newly diagnosed pemphigus vulgaris.

BACKGROUND: Pemphigus vulgaris (PV) is an autoimmune blistering disorder of the skin and/or mucosa. Increased levels of reactive oxygen species (ROS) were previously reported in PV. AIM: Because oxidative stress has an important role in the inflammatory process, we designed this study to evaluate the antioxidant status in patients with PV and to compare it with that of healthy controls (HCs). METHODS: In this case-control study, 43 newly diagnosed patients with PV were compared with 58 HCs. The severity of the disease was estimated according to Harman scores. Erythrocyte glutathione peroxidase (GPx), superoxide dismutase (SOD), CAT and serum malondialdehyde (MDA) activities and total antioxidant capacity were measured. Data were analyzed by independent t-test. RESULTS: Both groups were similar in sex, age and body mass index. Mean duration of disease was 5.6 months. Mean oral and skin severities were 1.79 and 2.3 respectively, based on Harman scores. SOD activity was not significantly different between groups (1003.30 ± 39.96 vs. 1009.76 ± 32.68 U/gHb). Levels were noticeably higher in patients with PV than in HCs for both GPx (52.13 ± 2.85 vs. 36.63 ± 1.49 U/gHb, respectively; P < 0.001) and CAT (205.69 ± 8.10 vs. 130.26 ± 6.80 kU/gHb, respectively; P < 0.001) activities, and CAT activity correlated with disease severity. In addition, patients had lower total antioxidant capacity than controls (3.39 ± 0.06 vs. 3.72 ± 0.09 mmol/L, P = 0.006). There was no noticeable difference in serum MDA between the two groups (P = 0.45). CONCLUSIONS: Patients with PV have significantly higher antioxidant enzyme activities and lower total antioxidant capacity compared with HCs. These data indicate the importance of improving antioxidant level in patients with pemphigus.

Effect of vitamins A, E, C and omega-3 fatty acids supplementation on the level of catalase and superoxide dismutase activities in streptozotocin-induced diabetic rats.

BACKGROUND: Since free radicals and antioxidant enzymes may play an important role in the development of diabetes, the present study was designed to assess the effect of supplementation with vitamins A, E and C and ω-3 fatty acids on catalase and superoxide dismutase activity in streptozotocin (STZ)-induced diabetic rats. METHODS: A total of 64 male Wistar rats weighing 250 g were divided into four groups as normal control, diabetic control, diabetic supplemented with vitamin A, E and C and diabetic supplemented with ω-3 fatty acids. After four weeks the rats were anesthetized and catalase (CAT) and superoxide dismutase (SOD) activities were investigated in blood samples, liver and heart homogenates. RESULTS: In diabetic rats, the activity levels of heart SOD (p < 0.001) and heart and liver CAT (p < 0.001) were significantly lower than in normal control rats. Supplementation with vitamins A, E and C significantly increased heart CAT (p = 0.05). No significant change was observed in diabetic rats supplemented with ω-3 fatty acids. CONCLUSION: Supplementation with vitamins A, E and C and ω-3 fatty acids was found to increase heart CAT activity in diabetic rats and they can be valuable candidates in the treatment of the complications of diabetes (Tab. 6, Ref. 26).

An endoplasmic reticulum trafficking signal regulates surface expression of ß4 subunit of a voltage- and Ca²âº-activated K⁺ channel.

Voltage-dependent and calcium-activated K⁺ (MaxiK, BK) channels are widely expressed in many tissues and organs where they play various physiological roles. Here we report discovery of a functional trafficking signal in MaxiK channel accessory ß4 subunit that could regulate activity of MaxiK α subunit (hSlo) on the plasma membrane. We demonstrate that ß4 is mostly retained within the cell and removal or mutation of ß4 trafficking signal significantly enhances its surface expression in HEK293T expression system. In hippocampal slices and cultured neurons we also observed significant ß4 expressions within the neurons. Finally, we show that unlike SV1 and ß1 subunits, ß4 shows no dominant-negative effect on MaxiK channel α subunit. Taken together, we propose ß4 subunit of MaxiK channel is mostly retained within the cells without interfering with other subunits. Removal of ß4 retention signal increases its surface expression that may lead to reduction of the MaxiK channel activity and neuronal excitability.