Link Security Situation Identification Method Based on the Ad Hoc Network of Medical Units.

In order to strengthen the management and security status monitoring of the internal network of medical units and make up for security vulnerabilities in time, an ad hoc network link security situation identification method is proposed. According to the architecture of the ad hoc network, it is analyzed that it has the advantages of strong persistence and its own protocol. Combined with the data of detection equipment and security log, the hierarchical acquisition model is used to obtain the situation elements such as port scanning attack and flood attack. The transmission rate factor, forwarding rate factor, dispersion factor, and node aggregation factor are regarded as eigenvectors. We determine the relationship between identity, difference, and opposition, identify the security situation through the description of the node state, and conduct quantitative processing to obtain the final identification result. The experimental results show that the weight value of this method is the same as the standard weight, which can identify the security situation level, obtain the specific situation value, and present a more intuitive identification result.

Exogenous H2S Protects Colon Cells in Ulcerative Colitis by Inhibiting NLRP3 and Activating Autophagy.

Hydrogen sulfide (H2S) has been reported to participate in intestinal mucosal defense and repair. However, the precise regulatory mechanisms of H2S in ulcerative colitis (UC) remain unclear. We explored the effects of sodium hydrosulfide (NaHS), a donor of H2S, in dextran sulfate sodium (DSS)-induced colitis in rats. The pathologic features were determined by analyzing the hematoxylin and eosin-stained samples. Interleukin-1ß (IL-1ß), IL-6, tumor necrosis factor-α (TNF-α), and myeloperoxidase (MPO) levels were determined using ELISA. The presence of cystathionine-γ-lyase (CSE) and light chain 3B (LC3B) were determined using immunohistochemical and immunofluorescence (IF) approaches, respectively. Next, we investigated the effects of NaHS in lipopolysaccharide (LPS)-stimulated human colonic smooth muscle cells (H2940). The level of reactive oxygen species (ROS) was determined using IF. NOD-like receptor 3 (NLRP3) and CSE were detected using western blot and quantitative real-time polymerase chain reaction. Autophagy was determined using western blot, IF, and electron microscopy. NaHS treatment considerably diminished colitis-induced histological injury and proinflammatory cytokine expressions. MPO, CSE, and H2S were downregulated, whereas LC3B was upregulated after NaHS administration in colitic rats. NaHS remarkably attenuated the levels of ROS, CSE, and NLRP3 in LPS-stimulated cells and enhanced autophagy, as was revealed by increased LC3-II-to-LC3-I ratio, elevated LC3, and decreased p62. Importantly, NaHS promoted autophagosome formation in LPS-treated cells. Exogenous H2S ameliorates intestinal injury by downregulating inflammation and activation of autophagy, suggesting the potential of NaHS as a therapeutic agent for UC.