Ku proteins promote DNA binding and condensation of cyclic GMP-AMP synthase.

Cyclic GMP-AMP synthase (cGAS) is a cytosolic DNA sensor that plays a critical role in regulating antiviral signaling. cGAS binds to DNA and catalyzes the synthesis of cyclic GMP-AMP (cGAMP), which is essential for downstream signal transduction. The antiviral response is a rapid biological process; however, cGAS itself has relatively low DNA binding affinity, implying that formation of the cGAS-DNA complex requires an additional factor(s) that promotes cGAS-DNA binding, allowing efficient antiviral signal transduction. Here, we report that the Ku proteins (Ku80 and Ku70) directly interact with cGAS and positively regulate cGAS-mediated antiviral signaling. Mechanistically, we find that the interaction of the Ku proteins with cGAS significantly increases the DNA-binding affinity of cGAS and promotes cGAS condensation in the cytosol, thereby enhancing cGAS catalytic activity. Our results show that the Ku proteins are critical partners of cGAS in sensing DNA virus infection and ensuring efficient innate immune signal transduction.

Separation of emulsified crude oil from produced water by gas flotation: A review.

The development and production of oil and gas fields would eventually result in a considerable amount of oily generated water, posing serious risks to humans and the environment. Nowadays, the oil concentration in the drainage stream of the produced water is strictly regulated, and many countries have established strict emission standards. As an indispensable oily wastewater treatment technology, flotation technology has attracted much attention because of its maturity, economy, practicality, and relative efficiency. Firstly, this paper summarizes and compares flotation techniques, such as dissolved gas flotation, induced gas flotation, electroflotation, and compact flotation units widely used in produced water treatment offshore in recent years. Considering the complexity of the mechanism of oil removal by air flotation, the mechanism of the oil droplet-bubble interaction is further discussed. The effects of flocculant, PH, and salinity on the oil droplet-bubble interaction in the flotation process were summarized from the perspective of the microscopic colloidal interface, which has a specific guiding role in improving the oil removal efficiency in the gas flotation process. Finally, the research status of produced water treatment by air flotation is summarized, and the feasible research direction is put forward.