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Circadian dysregulation involved in the pathophysiology of spinal cord injury (SCI). Modulation of circadian rhythms hold promise for the SCI treatment. Here, we aim to investigated the mechanism of olfactory ensheathing cells (OEC) in alleviating neuroinflammation via modulating clock gene expression in microglia. In this study, SCI rats were randomly divided into OEC group and vehicle group. At 1 day after the surgery, OECs were intravenously transplanted into OEC group SCI rat, while the rats in vehicle group received culture medium. After 7 days post of OEC transplantation, tissues were collected from the brain (prefrontal cortex, hypothalamus, spinal cord) for PCR, western blotting and immunohistochemistry (IHC) assay at zeitgeber time (ZT) 6, ZT 12, ZT 18, and ZT 24. The roles of OEC in modulating REV-ERBα in microglia were studied by experimental inhibition of gene expression and the co-culture experiment. In the vehicle group, IHC showed a significant increase of Iba-1 expression in the cerebral white matter and spinal cord compared with control group (P < 0.0001 for all comparisons). The expression of Iba-1 was significantly decreased (P < 0.0001 for all comparisons). In the OEC group, the expression of PER 1, PER 2, CLOCK, and REV-ERBα was in a rhythmical manner in both spinal cord and brain regions. SCI disrupted their typical rhythms. And OECs transplantation could modulate those dysregulations by upregulating REV-ERBα. In vitro study showed that OECs couldn't reduce the activation of REV-ERBα inhibited microglia. The intravenous transplantation of OECs can mediate cerebral and spinal microglia activation through upregulation REV-ERBα after SCI.
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Microglia , Ratos Sprague-Dawley , Traumatismos da Medula Espinal , Regulação para Cima , Animais , Traumatismos da Medula Espinal/terapia , Traumatismos da Medula Espinal/metabolismo , Microglia/metabolismo , Ratos , Doenças Neuroinflamatórias/metabolismo , Membro 1 do Grupo D da Subfamília 1 de Receptores Nucleares/metabolismo , Membro 1 do Grupo D da Subfamília 1 de Receptores Nucleares/genética , Masculino , Bulbo Olfatório/citologia , Bulbo Olfatório/metabolismoRESUMO
The analysis of influencing factors serves as the cornerstone for the research on smart community construction. Drawing upon both field research and extensive literature study, this paper explores the influencing factors of China's smart community construction and its effective paths by taking 52 national pilot zones for community governance and service innovation in China as examples. In the constructed analytical framework of influencing factors, elements such as economic development, capital investment, information infrastructure, community governance, public support system, and smart platform are included. By the use of the qualitative comparative analysis (QCA) method, the results of the study show that community governance, public support system, and smart platform are necessary conditions for smart community construction, while economic development, capital investment and information infrastructure play a leading role in the four combined paths. Finally, this study provides a new perspective for theoretical research, a reference forgovernmental departments to make decisions, and experience for the construction of smart communities in other developing countries.
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Green leaf volatiles (GLVs) are important in giving grape a fresh and green aroma. But the changes in GLVs during the phenological development of grapevines are not well known. This study analyzed the GLVs and transcription levels of associated biosynthetic genes in six grape species from the Loess Plateau region at five stages of maturation. Thirteen GLVs were detected, showing unique patterns for each grape type at various growth phases. The primary components in six grapes were (E)-2-hexenal, (E)-2-hexen-1-ol, and hexanal. With the exception of Cabernet Franc in 2019, the overall GLV contents of the six types generally increased during growth and development, peaking or stabilizing at harvest. And Sauvignon Blanc, Cabernet Gernischt, and Cabernet Sauvignon exhibited higher total contents among the varieties. PLS-DA analysis revealed 3-hexenal's high VIP scores across two years, underscoring its critical role in grape variety classification. Correlation analysis revealed a strong positive correlation between the levels of hexanal, 1-hexanol, (E)-2-hexen-1-ol, (Z)-3-hexenyl acetate, nonanal, and (E, E)-2,6-nonadienal and the expression of VvHPL and VvAAT genes in the LOX-HPL pathway. Specifically, VvHPL emerges as a potential candidate gene responsible for species-specific differences in GLV compounds. Comprehending the changing patterns in the biosynthesis and accumulation of GLVs offers viticulturists and enologists the opportunity to devise targeted strategies for improving the aromatic profile of grapes and wines.
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Objective.The rapid serial visual presentation (RSVP) paradigm, which is based on the electroencephalogram (EEG) technology, is an effective approach for object detection. It aims to detect the event-related potentials (ERP) components evoked by target images for rapid identification. However, the object detection performance within this paradigm is affected by the visual disparity between adjacent images in a sequence. Currently, there is no objective metric to quantify this visual difference. Consequently, a reliable image sorting method is required to ensure the generation of a smooth sequence for effective presentation.Approach. In this paper, we propose a novel semantic image sorting method for sorting RSVP sequences, which aims at generating sequences that are perceptually smoother in terms of the human visual experience.Main results. We conducted a comparative analysis between our method and two existing methods for generating RSVP sequences using both qualitative and quantitative assessments. A qualitative evaluation revealed that the sequences generated by our method were smoother in subjective vision and were more effective in evoking stronger ERP components than those generated by the other two methods. Quantitatively, our method generated semantically smoother sequences than the other two methods. Furthermore, we employed four advanced approaches to classify single-trial EEG signals evoked by each of the three methods. The classification results of the EEG signals evoked by our method were superior to those of the other two methods.Significance. In summary, the results indicate that the proposed method can significantly enhance the object detection performance in RSVP-based sequences.
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Eletroencefalografia , Potenciais Evocados Visuais , Estimulação Luminosa , Semântica , Humanos , Eletroencefalografia/métodos , Masculino , Feminino , Estimulação Luminosa/métodos , Adulto Jovem , Adulto , Potenciais Evocados Visuais/fisiologia , Reconhecimento Visual de Modelos/fisiologia , AlgoritmosRESUMO
Quantum ghost image technique utilizing position or momentum correlations between entangled photons can realize nonlocal reconstruction of the image of an object. In this work, based on polarization entanglement, we experimentally demonstrate quantum ghost imaging of vector images by using a geometric phase object. We also provide a corresponding theoretical analysis. Additionally, we offer a geometrical optics path explanation of ghost imaging for vector fields. The proposed strategy offers new insights into the fundamental development of ghost imaging and also holds great promise for developing complex structured ghost imaging techniques. Our work expanding the principle of ghost imaging to spatially varying vector beams will lead to interesting developments of this field.
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Sequential weak measurements allow for the direct extraction of individual density-matrix elements, rather than relying on global reconstruction of the entire density matrix, which opens a new avenue for the characterization of quantum systems. Nevertheless, extending the sequential scheme to multiqudit quantum systems is challenging due to the requirement of multiple coupling processes for each qudit and the lack of appropriate precision evaluation. To address these issues, we propose a resource-efficient scheme (RES) that directly characterizes the density matrix of general multiqudit systems while optimizing measurements and establishing a feasible estimation analysis. In the RES, an efficient observable of the quantum system is constructed such that a single meter state coupled to each qudit is sufficient to extract the corresponding density-matrix element. An appropriate model based on the statistical distribution of errors is utilized to evaluate the precision and feasibility of the scheme. We have experimentally applied the RES to the direct characterization of general single-photon qutrit states and two-photon entangled states. The results show that the RES outperforms sequential schemes in terms of efficiency and precision in both weak- and strong-coupling scenarios. This Letter sheds new light on the practical characterization of large-scale quantum systems and the investigation of their nonclassical properties.
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Changes in heart structure and function after lung resection in patients with lung cancer are challenging to manage. Therefore, a non-invasive and reliable measurement tool to gauge such changes is critical. The purpose of the present study was to compare cardiological changes before and after lung resection using tissue Doppler imaging (TDI). A total of 43 patients (19 men and 24 women) with primary non-small cell lung cancer (n=37) and metastatic cancer in the lungs (n=6) were enrolled in the study.nTDI was used to determine the thickness of the ascending aorta, the open size of the ascending valve, the anterior-oposterior diameters of the left atrium and left ventricle, and the thickness of the ventricular septum and right ventricle before and after lung resection. Left ventricular (LV) ejection fraction (EF), pulmonary valve flow rate, tricuspid annular or mitral leaflet tip early (E) peak/late (A) diastolic blood flow velocities, tricuspid regurgitation flow, the lateral mitral annulus early (e') diastolic velocity and mitral E/e' ratio were used to determine LV filling pressure. Results revealed no significant differences between male and female patients in terms of the open size of the ascending valve, the anterior-posterior diameter of the left ventricle and the mitral E/e' ratio. Significant differences were found in the width of the ascending aorta, anterior-posterior diameter of the left atrium, width of the LV septum and right ventricular (RV) diameter before and after lung resection. Finally, there were significantchanges in EF and tricuspid pressure. The results indicated that TDI was useful as a non-invasive method for assessing left and right heart function following lung resection. The LV and RV dimensions were affected, but LV filling pressure was preserved after lobectomy.
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Background: Circulating tumor cells (CTCs) could serve as a predictive biomarker in breast cancer (BC). Due to its high heterogeneity, the diagnostic and prognostic values of CTC are challenging. Methods: We searched published studies from the databases of PubMed, Cochrane Library, Embase, and MEDLINE. The detection capability and hazard ratios (HRs) of CTCs were extracted as the clinical diagnosis and prognosis evaluation. Subgroup analyses were divided according to the detection methods, continents, treatment periods, therapeutic plans, and cancer stages. Results: In this study, 35 publications had been retrieved with 8,935 patients enrolled. The diagnostic efficacy of CTC detection has 74% sensitivity and 98% specificity. The positive CTC detection (CTC+) would predict worse OS and PFS/DFS in both mid-therapy and post-therapy (HROS, 3.09; 95% CI, 2.17-4.39; HRPFS/DFS, 2.06; 95% CI, 1.72-2.47). Moreover, CTC+ indicated poor survival irrespective of the treatment phases and sampling times (HROS, 2.43; 95% CI, 1.85-3.19; HRPFS/DFS, 1.82; 95% CI, 1.66-1.99). The CTC+ was associated with poor survival regardless of the continents of patients (HROS = 2.43; 95% CI, 1.85-3.19). Conclusion: Our study suggested that CTC+ was associated with a worse OS and PFS/DFS in the Asian population. The detection method, the threshold level of CTC+, therapeutic approaches, and sampling times would not affect its diagnostic and prognostic values.
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INTRODUCTION: Smoking is an independent risk factor for the formation and rupture of intracranial aneurysms (IA). However, the underlying mechanism remains unclear. METHODS: In this study, we performed miRNA sequencing on plasma from 10 smoking patients with IA, 10 non-smoking patients with IA, and 10 healthy controls. The differentially expressed miRNAs (DE miRNAs) between smoking and non-smoking patients with IA were identified. Functional and pathway enrichment analysis is employed to investigate the potential functions of those DE miRNA target genes. The correlations with the clinical parameters were assessed using receiver operating characteristic curve (ROC) analysis. RESULTS: In total, we identified 428 DE miRNAs. Functional enrichment analysis showed the target genes were significantly enriched in biological aspects related to cell characteristics, such as cell cycle, cell differentiation, and cell migration. Pathway analysis showed DE miRNAs mainly enriched in the PI3K-Akt signaling pathway, Focal adhesion, and JAK-STAT signaling pathway. The expressions of miR-574-5p, miR-151a-3p, and miR-652-3p correlated well with aneurysm parameters. The AUC of miR-574-5p, miR-151a-3p, and miR-652-3p were 97%, 92%, and 99%, respectively. CONCLUSION: Our study indicated that smoking significantly altered the plasma miRNA profile in patients with IA. The expression of miR-574-5p, miR-151a-3p, and miR-652-3p correlated with aneurysm parameters, which may play a significant role in the formation and rupture of IA.
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Metamaterial perfect absorbers (MPAs) show great potential in achieving exceptional sensing performance, particularly in the realm of surface-enhanced infrared absorption (SEIRA) spectroscopy. To this aim, it is highly desirable for the localized hotspots to be readily exposed and accessible to analyte with strong mode confinement to enhance absorption. Here, we propose a quasi-three-dimensional MPA based on cross-shaped coupled complementary plasmonic arrays for highly sensitive refractive index sensing and molecular vibrational sensing. Dual-band perfect absorption can be approached with the two plasmonic resonances corresponding to the electric dipole-like mode of cross antenna array and the magnetic dipole-like mode of cross hole array, respectively. Large portions of the electric field of the hotspots are exposed and concentrated in the gap between the elevated cross antenna and its complementary structure on the substrate, leading to improved sensing sensitivities. An ultrathin polymethyl methacrylate (PMMA) film induces a significant redshift of the magnetic dipole-like mode with an 11.8â nm resonance shift per each nanometer polymer thickness. The value is comparable to the reported sensitivity of single molecule layer sensors. Additionally, the simultaneous detection of the C = O and C-H vibrations of PMMA molecules is enabled with the two plasmonic resonances adjusted by changing the lengths of the two cross branches. Remarkably, the observed mode splitting and anti-crossing behavior imply the strong interaction between plasmonic resonance and molecular vibration. Our dual-band MPA based on coupled complementary plasmonic arrays opens a new avenue for developing highly sensitive sensors for the detection of refractive index and multispectral molecular vibrations.
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Brain metastases (BMs) usually develop in patients with non-small cell lung cancer. In addition to systemic therapy, radiation therapy and surgery, anti-programmed cell death-ligand 1 (PD-L1) therapy is another promising clinical anticancer treatment modality. However, the optimal timing and drug-drug interactions of anti-PD-L1 therapy with other combined treatments remain to be elucidated. Treatment with anti-PD-L1 therapy is associated with an increased risk of radionecrosis (RN) regardless of tumor histology. The present study described a case of RN in a patient with lung adenocarcinoma and with BM who received anti-PD-L1 therapy. Before anti-PD-L1 treatment, the patient received whole brain radiotherapy. During durvalumab treatment, the intracranial metastases regressed. The progression of intracranial lesions 9 months later prompted a second-line of therapy with PD-L1 inhibitor durvalumab and stereotactic radiotherapy (SRT). Despite stereotactic irradiation, the lesions progressed further, leading to surgical resection. On examination, RN was detected, but there was no evidence of metastatic lung cancer. The aim of the present study was to present the longitudinal change in magnetic resonance imaging in RN following STR and anti-PD-L1 combined therapy. The atypical image of RN is conditionally important for making an accurate preoperative diagnosis.
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Superconducting nanocircuits, which are usually fabricated from superconductor films, are the core of superconducting electronic devices. While emerging transition-metal dichalcogenide superconductors (TMDSCs) with exotic properties show promise for exploiting new superconducting mechanisms and applications, their environmental instability leads to a substantial challenge for the nondestructive preparation of TMDSC nanocircuits. Here, we report a universal strategy to fabricate TMDSC nanopatterns via a topotactic conversion method using prepatterned metals as precursors. Typically, robust NbSe2 meandering nanowires can be controllably manufactured on a wafer scale, by which a superconducting nanowire circuit is principally demonstrated toward potential single photon detection. Moreover, versatile superconducting nanocircuits, e.g., periodical circle/triangle hole arrays and spiral nanowires, can be prepared with selected TMD materials (NbS2, TiSe2, or MoTe2). This work provides a generic approach for fabricating nondestructive TMDSC nanocircuits with precise control, which paves the way for the application of TMDSCs in future electronics.
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Prostate-specific antigen (PSA) test is widely used to diagnose early prostate cancer (PCa). Its low sensitivity, especially in the gray zone, usually incurs overtreatment or missed diagnosis. As an emerging tumor marker, exosomes have attracted great interest in non-invasive diagnosis of PCa. However, the quick direct detection of exosomes in serum is still a big challenge for convenient screening of early PCa due to their high-degree heterogeneity and complexity. Here we develop the label-free biosensors based on wafer-scale plasmonic metasurfaces, and establish a flexible spectral methodology of exosomes profiling, which facilitates their identification and quantification in serum. We combine the metasurfaces functionalized by anti-PSA and anti-CD63, respectively, and build a portable immunoassay system to detect serum PSA and exosomes simultaneously within 20 min. Our scheme can discriminate early PCa from benign prostatic hyperplasia with a diagnostic sensitivity of 92.3%, which is much higher that of 58.3% for conventional PSA tests. The receiver operating characteristic analysis in clinical trials demonstrates significant PCa distinguishing capability with an area under the curve up to 99.4%. Our work provides a rapid and powerful approach for precise diagnosis of early PCa, and will inspire more exosomes metasensing studies for other early cancer screening.
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Técnicas Biossensoriais , Exossomos , Hiperplasia Prostática , Neoplasias da Próstata , Masculino , Humanos , Antígeno Prostático Específico , Exossomos/patologiaRESUMO
Brain metastasis (BM) is a critical cause of morbidity and mortality in patients with breast cancer (BC). Compared with other cancer cells, BC cells (BCs) exhibit special features in the metastatic process. However, the underlying mechanisms are still unclear, especially the crosstalk between tumour cells and the microenvironment. To date, novel therapies for BM, including targeted therapy and antibodyâdrug conjugates, have been developed. Due to an improved understanding of the bloodâbrain barrier (BBB) and blood-tumour barrier (BTB), the development and testing of therapeutic agents in clinical phases have substantially increased. However, these therapies face a major challenge due to the low penetration of the BBB or BTB. As a result, researchers have increasingly focused on finding ways to promote drug penetration through these barriers. This review provides an updated overview of breast cancer brain metastases (BCBM) and summarizes the newly developed therapies for BCBM, especially drugs targeting the BBB or BTB.
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Neoplasias Encefálicas , Neoplasias da Mama , Humanos , Feminino , Neoplasias da Mama/patologia , Barreira Hematoencefálica , Neoplasias Encefálicas/patologia , Microambiente Tumoral , BiologiaRESUMO
Superconducting photodetection offers a wide spectral coverage ranging from the microwave to X-ray, and in the short wavelength range, single photon sensitivity can be achieved. However, in the longer wavelength infrared region, the system detection efficiency is low due to the lower internal quantum efficiency and weak optical absorption. Here, we utilized the superconducting metamatieral to enhance the light coupling efficiency and reach nearly perfect absorption at dual color infrared wavelengths. Dual color resonances arise from hybridization of local surface plasmon mode of the metamaterial structure and the Fabry-Perot-like cavity mode of metal (Nb)-dielectric (Si)-metamatieral (NbN) tri-layer structure. We demonstrated that, at the working temperature of 8â K slightly below TC â¼8.8â K, this infrared detector exhibits the peak responsivity of 1.2 × 106V/W and 3.2 × 106V/W at two resonant frequencies 36.6 THz and 104 THz, respectively. The peak responsivity is enhanced about â¼8 and â¼22 times, respectively, compared to that of non-resonant frequency (67 THz). Our work provides a way to harvest infrared light efficiently and hence improve the sensitivity of superconducting photodetectors in multispectral infrared range, which may find promising applications in thermal image and gas sensing etc.
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The characterization and manipulation of polarization state at single photon level are of great importance in research fields such as quantum information processing and quantum key distribution, where photons are normally delivered using single mode optical fibers. To date, the demonstrated polarimetry measurement techniques based on a superconducting nanowire single photon detector (SNSPD) require the SNSPD to be either highly sensitive or highly insensitive to the photon's polarization state, therefore placing an unavoidable challenge on the SNSPD's design and fabrication processes. In this article, we present the development of an alternative polarimetry measurement technique, of which the stringent requirement on the SNSPD's polarization sensitivity is removed. We validate the proposed technique by a rigorous theoretical analysis and comparisons of the experimental results obtained using a fiber-coupled SNSPD with a polarization extinction ratio of â¼2 to that obtained using other well-established known methods. Based on the full Stokes data measured by the proposed technique, we also demonstrate that at the single photon level (â¼ -100 dBm), the polarization state of the photon delivered to the superconducting nanowire facet plane can be controlled at will using a further developed algorithm. Note that other than the fiber-coupled SNSPD, the only component involved is a quarter-wave plate (no external polarizer is necessary), which when aligned well has a paid insertion loss less than 0.5â dB.
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Electron beam lithography uses an accelerated electron beam to fabricate patterning on an electron-beam-sensitive resist but requires complex dry etching or lift-off processes to transfer the pattern to the substrate or film on the substrate. In this study, etching-free electron beam lithography is developed to directly write a pattern of various materials in all-water processes, achieving the desired semiconductor nanopatterns on a silicon wafer. Introduced sugars are copolymerized with metal ions-coordinated polyethylenimine under the action of electron beams. The all-water process and thermal treatment result in nanomaterials with satisfactory electronic properties, indicating that diverse on-chip semiconductors (e.g., metal oxides, sulfides, and nitrides) can be directly printed on-chip by an aqueous solution system. As a demonstration, zinc oxide patterns can be achieved with a line width of 18 nm and a mobility of 3.94 cm2 V-1 s-1. This etching-free electron beam lithography strategy provides an efficient alternative for micro/nanofabrication and chip manufacturing.
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Obtaining high-quality absorber layers is a major task for constructing efficient thin-film solar cells. Hydrothermal deposition is considered a promising method for preparing high-quality antimony sulfide (Sb2 S3 ) films for solar cell applications. In the hydrothermal process, the precursor reactants play an important role in controlling the film formation process and thus the film quality. In this study, Sb2 O3 is applied as a new Sb source to replace the traditional antimony potassium tartrate to modulate the growth process of the Sb2 S3 film. The reaction mechanism of the transition from Sb2 O3 to Sb2 S3 in the hydrothermal process is revealed. Through controlling the nucleation and deposition processes, high-quality Sb2 S3 films are prepared with longer carrier lifetimes and lower deep-level defect densities than those prepared from the traditional Sb source of antimony potassium tartrate. Consequently, a solar cell device based on this improved Sb2 S3 delivers a high power conversion efficiency of 6.51 %, which is in the top tier for Sb2 S3 -based solar devices using hydrothermal methods. This research provides a new and competitive Sb source for hydrothermal growth of high-quality antimony chalcogenide films for solar cell applications.
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OBJECTIVE: This study presents a novel brain-computer interface paradigm of dual-frequency modulated steady-state visual evoked potential (SSVEP), aiming to suppress the unpredictable intermodulation components in current applications. This paradigm is especially suitable for training-free scenarios. APPROACH: This study built a dual-frequency binocular vision SSVEP brain-computer interface system using circularly polarized light technology. Two experiments, including a 6-target offline experiment and a 40-target online experiment, were taken with this system. Meanwhile, an improved algorithm filter bank dual-frequency canonical correlation analysis (FBDCCA) was presented for the dual-frequency SSVEP paradigm. MAIN RESULTS: Energy analysis was conducted for 9 subjects in the 6-target dual-frequency offline experiment, among which the signal-to-noise ratio of target frequency components have increased by 2 dB compared to the one of unpredictable intermodulation components. Subsequently, the online experiment with 40 targets was conducted with 12 subjects. With this new dual-frequency paradigm, the online training-free experiment's average information transmission rate (ITR) reached 104.56 ± 15.74 bits/min, which was almost twice as fast as the current best dual-frequency paradigm. And the average information transfer rate for offline training analysis of this new paradigm was 180.87 ± 17.88 bits/min. SIGNIFICANCE: These results demonstrate that this new dual-frequency SSVEP brain-computer interface paradigm can suppress the unpredictable intermodulation harmonics and generate higher quality responses while completing dual-frequency encoding. Moreover, its performance shows high ITR in applications both with and without training. It is thus believed that this paradigm is competent for achieving large target numbers in brain-computer interface systems and has more possible practices.