Enhanced denitrification by graphene oxide-modified cathode for the secondary effluent of wastewater treatment plants in three-dimensional biofilm electrode reactors.

In this study, a novel three-dimensional biofilm electrode reactor (3D-BER) with a graphene oxide (GO)-modified cathode was developed to enhance the denitrification performance of secondary effluent from wastewater treatment plants (SEWTPs). The effects of different hydraulic retention times (HRTs) and currents on the 3D-BER were explored. The results indicated that at the optimal HRT of 4 h and current of 350 mA/m2, the 3D-BER with GO-modified cathode had a higher denitrification rate (2.40 ± 0.1 mg TN/L/h) and less accumulation of intermediate products, especially with 3.34% total nitrogen (TN) molar conversion to N2O. The GO-modified cathode offered a large biocompatible specific surface area and enhanced the conductivity, which favored microbial growth and increased electron transfer efficiency and extracellular enzyme activities. Moreover, the activity of nitrite reductase increased more than that of nitrate reductase to accelerate nitrite reduction, thus facilitating the denitrification process. The proposed 3D-BER provided an effective solution to elevate tertiary denitrification in the SEWTP.

Revisiting iodide species transformation in peracetic acid oxidation: unexpected role of radicals in micropollutants decontamination and iodate formation.

Peracetic acid (PAA) is an alternative disinfectant for saline wastewaters, and hypohalous acids are typically regarded as the reactive species for oxidation and disinfection. However, new results herein strongly suggest that reactive radicals instead of HOI primarily contributed to decontamination during PAA treatment of iodine-containing wastewater. The presence of I- could greatly accelerate the micropollutants (e.g., sulfamethoxazole (SMX)) transformation by PAA. Chemical probes experiments and electron paramagnetic resonance analysis demonstrate acetylperoxyl radical rather than reactive iodine species primarily responsible for SMX degradation. The kinetic model was developed to further distinguish and quantify the contribution of radicals and iodine species, as well as to elucidate the transformation pathways of iodine species. Density functional theory calculations indicated that the nucleophilic attack of I- on the peroxide bond of PAA could form unstable O-I bond, with the transition state energy barrier for radical generation lower than that for HOI formation. The transformation of iodine species was regulated by acetylperoxyl radical to generate nontoxic IO3-, greatly alleviating the iodinated DBPs formation in saline wastewaters. This work provides mechanistic insights in radical-regulated iodine species transformation during PAA oxidation, paving the way for the development of viable and eco-friendly technology for iodide containing water treatment.

Revisiting the synergistic oxidation of peracetic acid and permanganate(â ¦) towards micropollutants: The enhanced electron transfer mechanism of reactive manganese species.

Synergistic actions of peroxides and high-valent metals have garnered increasing attentions in wastewater treatment. However, how peroxides interact with the reactive metal species to enhance the reactivity remains unclear. Herein, we report the synergistic oxidation of peracetic acid (PAA) and permanganate(Ⅶ) towards micropollutants, and revisit the underlying mechanism. The PAA-Mn(VII) system showed remarkable efficiency with a 28-fold enhancement on sulfamethoxazole (SMX) degradation compared to Mn(Ⅶ) alone. Extensive quenching experiments and electron spin resonance (ESR) analysis revealed the generation of unexpected Mn(V) and Mn(VI) beyond Mn(III) in the PAA-Mn(VII) system. The utilization efficiency of Mn intermediates was quantified using 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonate (ABTS), and the results indicated that PAA could enhance the electron transfer efficiency of reactive manganese (Mn) species, thus accelerating the micropollutant degradation. Density functional theory (DFT) calculations showed that Mn intermediates could coordinate to the O1 of PAA with a low energy gap, enhancing the oxidation capacity and stability of Mn intermediates. A kinetic model based on first principles was established to simulate the time-dependent concentration profiles of the PAA-Mn complexes and quantify the contributions of the PAA-Mn(III) complex (50.8 to 59.3 %) and the PAA-Mn(Ⅴ/Ⅵ) complex (40.7 to 49.2 %). The PAA-Mn(VII) system was resistant to the interference from complex matrix components (e.g., chloride and humic acid), leading to the high efficiency in real wastewater. This work provides new insights into the interaction of PAA with reactive manganese species for accelerated oxidation of micropollutants, facilitating its application in wastewater treatment.

Chloride-mediated enhancement in Cu(II)-catalyzed Fenton-like reaction: The overlooked reactive chlorine species.

The practical application of Cu(II)-catalyzed Fenton-like reaction (Cu(II)/H2O2) exhibits a low efficiency in the degradation of refractory compounds of wastewater. The impact of chloride ions (Cl-) on Fenton-like reactions have been investigated, but the influence mechanism is still unclear. Herein, the presence of Cl- (5 mM) significantly accelerated the degradation of benzoic acid (BA) under neutral conditions. The degradation of BA follows pseudo-first-order kinetics, with a degradation rate 7.3 times higher than the Cu(II)/H2O2 system. Multiple evidences strongly demonstrated that this reaction enables the production of reactive chlorine species (RCS) rather than HO• and high-valent copper (Cu(III)). The kinetic model revealed that Cl- could shift reactive species from the key intermediate (Cu(III)-chloro complexes) to RCS. Dichlorine radicals (Cl2•-) was discovered to play a crucial role in BA degradation, which was largely overlooked in previous reports. Although the reaction rate of Cl2•- with BA (k = 2.0 × 106 M-1 s-1) is lower than that of other species, its concentration is 10 orders of magnitude higher than that of Cu(III) and HO•. Furthermore, the exceptional efficacy of the Cu(II)/H2O2 system in BA degradation was observed in saline aquatic environments. This work sheds light on the previously unrecognized role of the metal-chloro complexes in production the RCS and water purification.

La3+@BC500-S2O82- system for removal of sulfonamide antibiotics in water.

Sulfonamide antibiotics (SAs) widely used have potentially negative effects on human beings and ecosystems. Adsorption and advanced oxidation methods have been extensively applied in SAs wastewater treatment. In this study, compared with Al3+@BC500 and Fe3+@BC500, La3+@BC500 for activating persulfate (S2O82-) had the best effect removal performance of sulfadiazine (SDZ) and sulfamethoxazole (SMX). Morphology, acidity, oxygen-containing functional groups, and loading of La3+@BC500 were analyzed by techniques, including EA, BET, XRD, XPS, FT-IR. XRD results show that with the increase of La3+ loading, the surface characteristics of biochar gradually changed from CaCO3 to LaCO3OH. Through EPR technology, it is proved that LaCO3OH on the surface of La3+@BC500 can not only activate S2O82- to generate SO4-•, but also to produce •OH. In the optimization experiment, the optimal dosage of La3+ is between 0.05 and 0.2 (mol/L)/g. SDZ had a good removal effect at pH (5-9), but SMX had a good removal effect only at pH=3. Zeta potential also proves that the material is more stable under acidic conditions. The removal process of SDZ is more in accord with pseudo-first-order kinetics (R2=0.9869), while SMX is more in line with pseudo-second order kinetics (R2=0.9926).

Maximizing the wetting resistance of fluorine-free omniphobic membranes for hypersaline wastewater desalination.

Membrane distillation (MD) equipped with omniphobic (non-wetting) membranes has found a niche in water reclamation from hypersaline industrial wastewater. Here, we examined the efficacy of non-fluorinated materials as surface coating agents for omniphobic MD membrane fabrication, and identified necessary mechanisms to attain a maximized wetting resistance using fluorine-free materials. We first prepared MD membranes with different surface chemistries using a series of linear alkylsilanes and polydimethylsiloxane (PDMS) as representative fluorine-free, low surface energy materials. Membranes modified with a longer chain alkylsilane exhibited a lower surface energy and demonstrated a greater wetting resistance in direct contact MD experiments using feedwaters of various surface tensions. Despite the nearly identical surface energy measured for the longest alkylsilane and PDMS, PDMS-modified membrane exhibited an extended antiwetting performance as compared to the membrane treated with the longest alkylsilane. To elucidate the source of the distinctive wetting resistance, we examined the nucleation and condensation kinetics on the surfaces with the different surface chemistries via environmental scanning electron microscopy. Our analysis suggests that the membranes treated with long chain alkylsilanes contain surface defects (i.e., hydrophilic regions) whereas the high mobility of the PDMS effectively minimizes the defect exposure, slowing down the condensation and subsequent surface wetting.

Viral and Bacterial Community Dynamics in Food Waste and Digestate from Full-Scale Biogas Plants.

Anaerobic digestion (AD) is commonly used in food waste treatment. Prokaryotic microbial communities in AD of food waste have been comprehensively studied. The role of viruses, known to affect microbial dynamics and metabolism, remains largely unexplored. This study employed metagenomic analysis and recovered 967 high-quality viral bins within food waste and digestate derived from 8 full-scale biogas plants. The diversity of viral communities was higher in digestate. In silico predictions linked 20.8% of viruses to microbial host populations, highlighting possible virus predators of key functional microbes. Lineage-specific virus-host ratio varied, indicating that viral infection dynamics might differentially affect microbial responses to the varying process parameters. Evidence for virus-mediated gene transfer was identified, emphasizing the potential role of viruses in controlling the microbiome. AD altered the specific process parameters, potentially promoting a shift in viral lifestyle from lysogenic to lytic. Viruses encoding auxiliary metabolic genes (AMGs) were involved in microbial carbon and nutrient cycling, and most AMGs were transcriptionally expressed in digestate, meaning that viruses with active functional states were likely actively involved in AD. These findings provided a comprehensive profile of viral and bacterial communities and expanded knowledge of the interactions between viruses and hosts in food waste and digestate.

Non-phytoremediation and phytoremediation technologies of integrated remediation for water and soil heavy metal pollution: A comprehensive review.

Since the 1980s, there has been increasing concern over heavy metal pollution remediation. However, most research focused on the individual remediation technologies for heavy metal pollutants in either soil or water. Considering the potential migration of these pollutants, it is necessary to explore effective integrated remediation technologies for soil and water heavy metals. This review thoroughly examines non-phytoremediation technologies likes physical, chemical, and microbial remediation, as well as green remediation approaches involving terrestrial and aquatic phytoremediation. Non-phytoremediation technologies suffer from disadvantages like high costs, secondary pollution risks, and susceptibility to environmental factors. Conversely, phytoremediation technologies have gained significant attention due to their sustainable and environmentally friendly nature. Enhancements through chelating agents, biochar, microorganisms, and genetic engineering have demonstrated improved phytoremediation remediation efficiency. However, it is essential to address the environmental and ecological risks that may arise from the prolonged utilization of these materials and technologies. Lastly, this paper presents an overview of integrated remediation approaches for addressing heavy metal contamination in groundwater-soil-surface water systems and discusses the reasons for the research gaps and future directions. This paper offers valuable insights for comprehensive solutions to heavy metal pollution in water and soil, promoting integrated remediation and sustainable development.

Exposure Pathways and Toxicity of Microplastics in Terrestrial Insects.

The detrimental effects of plastics on aquatic organisms, including those of macroplastics, microplastics, and nanoplastics, have been well established. However, knowledge on the interaction between plastics and terrestrial insects is limited. To develop effective strategies for mitigating the impact of plastic pollution on terrestrial ecosystems, it is necessary to understand the toxicity effects and influencing factors of plastic ingestion by insects. An overview of current knowledge regarding plastic ingestion by terrestrial insects is provided in this Review, and the factors influencing this interaction are identified. The pathways through which insects interact with plastics, which can lead to plastic accumulation and microplastic transfer to higher trophic levels, are also discussed using an overview and a conceptual model. The diverse impacts of plastic exposure on insects are discussed, and the challenges in existing studies, such as a limited focus on certain plastic types, are identified. Further research on standardized methods for sampling and analysis is crucial for reliable research, and long-term monitoring is essential to assess plastic trends and ecological impacts in terrestrial ecosystems. The mechanisms underlying these effects need to be uncovered, and their potential long-term consequences for insect populations and ecosystems require evaluation.

New insight into the spatio-temporal patterns of functional groups of hotspot inside the composting aggregates by synchrotron-based FTIR in hyperthermophilic composting.

Hyperthermophilic composting (HTC) is a recently developed and highly promising organic fraction of municipal solid waste (OFMSW) treatment technology. Investigation of organic matter (OM) dynamics in compost particle is thus crucial for the understanding of humification of HTC process. Herein, this work aimed to study the chemical and structural changes of OM at the molecular level during HTC of OFMSW using EEM and SR-FTIR analyses. Additionally, two-dimensional correlation spectroscopy (2D-COS) was also utilized to probe and identify the changes in chemical constituents and functional groups of organic compounds on the surface of compost particles during different composting periods. Results show that SR-FTIR can detect fine-scale (~µm) changes in functional groups from the edges to the interior of compost particles during different composting periods by mapping the particles in situ. In the hyperthermophilic stage (day 9), the extracted µ-FTIR spectrum reveals a distinct boundary between anaerobic and aerobic regions within the compost particle, with a thickness of anaerobic zone (1460 cm-1) of approximately 30 µm inside the particle's core. This provides direct evidence of anaerobic trends at compost microscales level within compost particles. 2D-COS analysis indicated that organic functional groups gradually agglomerated in the order of 1330 > 2930 > 3320 > 1600 > 1030 > 895 cm-1 to the core skeleton of cellulose degradation residues, forming compost aggregates with well physicochemical properties. Overall, the first combination of SR-FTIR and EEM provides complementary explanations for the humification mechanism of HTC, potentially introducing a novel methodology for investigating the environmental behaviors and fates of various organic contaminants associated with OM during the in-situ composting biochemical process.

Nanobubbles improve peroxymonosulfate-based advanced oxidation: High efficiency, low toxicity/cost, and novel collaborative mechanism.

Cl- activated peroxymonosulfate (PMS) oxidation technology can effectively degrade pollutants, but the generation of chlorinated disinfection byproducts (DBPs) limits the application of this technology in water treatment. In this study, a method of nanobubbles (NBs) synergistic Cl-/PMS system was designed to try to improve this technology. The results showed the synergistic effects of NBs/Cl-/PMS were significant and universal while its upgrade rate was from 12.89% to 34.97%. Moreover, the synergistic effects can be further improved by increasing the concentration and Zeta potential of NBs. The main synergistic effects of NBs/Cl-/PMS system were due to the electrostatic attraction of negatively charged NBs to Na+ from NaCl, K+ from PMS, and H+ from phenol, which acted as a "bridge" between Cl- and HSO5- as well as phenol and Cl-/HSO5-, increasing active substance concentration. In addition, the addition of NBs completely changed the oxidation system of Cl-/PMS from one that increases environmental toxicity to one that reduces it. The reason was that the electrostatic attraction of NBs changed the active sites and degradation pathway of phenol, greatly reducing the production of highly toxic DBPs. This study developed a novel environmentally friendly oxidation technology, which provides an effective strategy to reduce the generation of DBPs in the Cl-/PMS system.

Generation and Fate of Nanoplastics in the Intestine of Plastic-Degrading Insect (Tenebrio molitor Larvae) during Polystyrene Microplastic Biodegradation.

The insect Tenebrio molitor exhibits ultrafast efficiency in biodegrading polystyrene (PS). However, the generation and fate of nanoplastics (NPs) in the intestine during plastic biodegradation remain unknown. In this study, we investigated the biodegradation of PS microplastics (MPs) mediated by T. molitor larvae over a 4-week period and confirmed biodegradation by analyzing Δδ13C in the PS before and after biotreatment (-28.37‰ versus -24.88‰) as an effective tool. The ·OH radicals, primarily contributed by gut microbiota, and H2O2, primarily produced by the host, both increased after MP digestion. The size distribution of residual MP particles in excrements fluctuated within the micrometer ranges. PS NPs were detected in the intestine but not in the excrements. At the end of Weeks 1, 2, 3, and 4, the concentrations of PS NPs in gut tissues were 3.778, 2.505, 2.087, and 2.853 ng/lava, respectively, while PS NPs in glands were quantified at 0.636, 0.284, and 0.113 ng/lava and eventually fell below the detection limit. The PS NPs in glands remained below the detection limit at the end of Weeks 5 and 6. This indicates that initially, NPs generated in the gut entered glands, then declined gradually and eventually disappeared or possibly biodegraded after Week 4, associated with the elevated plastic-degrading capacities of T. molitor larvae. Our findings unveil rapid synergistic MP biodegradation by the larval host and gut microbiota, as well as the fate of generated NPs, providing new insights into the risks and fate associated with NPs during invertebrate-mediated plastic biodegradation.

The aging of microplastics exacerbates the damage to photosynthetic performance and bioenergy production in microalgae (Chlorella pyrenoidosa).

The toxicity of microplastics (MPs) on freshwater plants has been widely studied, yet the influence of aged MPs remains largely unexplored. Herein, we investigated the influence of polyvinyl chloride (PVC) MPs, both before and after aging, at different environmentally relevant concentrations on Chlorella pyrenoidosa, a freshwater microalgae species widely recognized as a valuable biomass resource. During a 96-h period, both virgin and aged MPs hindered the growth of C. pyrenoidosa. The maximum growth inhibition rates were 32.40 % for virgin PVC at 250 mg/L and 44.72 % for aged PVC at 100 mg/L, respectively. Microalgae intracellular materials, i.e., protein and carbohydrate contents, consistently decreased after MP exposure, with more pronounced inhibition observed with aged PVC. Meanwhile, the MP aging significantly promoted the nitrogen uptake of C. pyrenoidosa, i.e., 1693.45 ± 42.29 mg/L (p < 0.01), contributing to the production of humic acid-like substances. Additionally, aged PVC induced lower chlorophyll a and Fv/Fm when compared to virgin PVC, suggesting a more serious inhibition of the photosynthesis process of microalgae. The toxicity of MPs to C. pyrenoidosa was strongly associated with intercellular oxidative stress levels. The results indicate that MP aging exacerbates the damage to photosynthetic performance and bioenergy production in microalgae, providing critical insights into the toxicity analysis of micro(nano)plastics on freshwater plants.

Effective defluorination of novel hexafluoropropylene oxide oligomer acids under mild conditions by UV/sulfite/iodide: mechanisms and ecotoxicity.

It has recently been discovered that HFPO-TA (a processing aid in the production of fluoropolymers) has high levels of bioaccumulation and biotoxicity. Hydrated electrons (eaq-) have been proposed to be potent nucleophiles that may decompose PFAS. Unlike previous studies in which the generation of eaq- was often restricted to anaerobic or highly alkaline environments, in this study, we applied the UV/SO32-/I- process under mild conditions of neutrality, low source chemical demand, and open-air, which achieved effective degradation (81.92 %, 0.834 h-1) and defluorination (48.99 %, 0.312 h-1) of HFPO-TA. With I- as the primary source of eaq-, SO32- acting as an I- regenerator and oxidizing substances scavenger, UV/SO32-/I- outperformed others under mild circumstances. The eaq- were identified as the main active species by quenching experiments and electron paramagnetic resonance (EPR). During degradation, the first site attacked by eaq- was the ether bond (C6-O7), followed by the generation of HFPO-DA, TFA, acetic and formic acid. Degradation studies of other HFPOs have shown that the defluorination of HFPOs was accompanied by a clear chain-length correlation. At last, toxicological experiments confirmed the safety of the process. This study updated our understanding of the degradation of newly PFASs and the application of eaq- mediated photoreductive approaches under mild conditions.

Interaction Effects between the Main Components of Protein-Rich Biomass during Microwave-Assisted Pyrolysis.

The interaction effects between the main components (proteins (P), carbohydrates (C), and lipids (L)) of protein-rich biomass during microwave-assisted pyrolysis were investigated in depth with an exploration of individual pyrolysis and copyrolysis (PC, PL, and CL) of model compounds. The average heating rate of P was higher than those of C and L, and the interactions in all copyrolysis groups reduced the max instant heating rate. The synergistic extent (S) of PC and PL for bio-oil yield was 16.78 and 18.24%, respectively, indicating that the interactions promoted the production of bio-oil. Besides, all of the copyrolysis groups exhibited a synergistic effect on biochar production (S = 19.43-28.24%), while inhibiting the gas generation, with S ranging from -20.17 to -6.09%. Regarding the gaseous products, apart from H2, P, C, and L primarily generated CO2, CO, and CH4, respectively. Regarding bio-oil composition, the interactions occurring within PC, PL, and CL exhibited a significantly synergistic effect (S = 47.81-412.96%) on the formation of N-heterocyclics/amides, amides/nitriles, and acids/esters, respectively. Finally, the favorable applicability of the proposed interaction effects was verified with microalgae. This study offers valuable insights for understanding the microwave-assisted pyrolysis of protein-rich biomass, laying the groundwork for further research and process optimization.

Enhanced adsorption of dye wastewater by low-temperature combined NaOH/urea pretreated hydrochar: Fabrication, performance, and mechanism.

The highly stable biomass structure formed by cellulose, hemicellulose, and lignin results in incomplete conversion and carbonization under hydrothermal conditions. In this study, pretreated corn straw hydrochar (PCS-HC) was prepared using a low-temperature alkali/urea combination pretreatment method. The Mass loss rate of cellulose, hemicellulose, and lignin from pretreated biomass, as well as the effects of the pretreatment method on the physicochemical properties of PCS-HC and the adsorption performance of PCS-HC for alkaline dyes (rhodamine B and methylene blue), were investigated. The results showed that the low-temperature NaOH/urea pretreatment effectively disrupted the stable structure formed by cellulose, hemicellulose, and lignin. NaOH played a dominant role in solubilizing cellulose and the combination of low temperature and urea enhanced the ability of NaOH to remove cellulose, hemicellulose, and lignin. Compared to the untreated hydrochar, PCS-HC exhibited a rougher surface, a more abundant pore structure, and a larger specific surface area. The unpretreated hydrochar exhibited an adsorption capacity of 64.8% for rhodamine B and 66.32% for methylene blue. However, the removal of rhodamine B and methylene blue by PCS-BC increased to 89.12% and 90.71%, respectively, under the optimal pretreatment conditions. The PCS-HC exhibited a favorable adsorption capacity within the pH range of 6-9. However, the presence of co-existing anions such as Cl-, SO42-, CO32-, and NO3- hindered the adsorption capacity of PCS-HC. Among these anions, CO32- exhibited the highest level of inhibition. Chemisorption, including complexation, electrostatic attraction, and hydrogen bonding, were the primary mechanism for dye adsorption by PCS-HC. This study provides an efficient method for utilizing agricultural waste and treating dye wastewater.

Enzyme modified biodegradable plastic preparation and performance in anaerobic co-digestion with food waste.

A modified biodegradable plastic (PLA/PBAT) was developed by through covalent bonding with proteinase K, porcine pancreatic lipase, or amylase, and was then investigated in anaerobic co-digestion mixed with food waste. Fluorescence microscope validated that enzymes could remain stable in modified the plastic, even after co-digestion. The results of thermophilic anaerobic co-digestion showed that, degradation of the plastic modified with Proteinase K increased from 5.21 ± 0.63 % to 29.70 ± 1.86 % within 30 days compare to blank. Additionally, it was observed that the cumulative methane production increased from 240.9 ± 0.5 to 265.4 ± 1.8 mL/gVS, and the methane production cycle was shortened from 24 to 20 days. Interestingly, the kinetic model suggested that the modified the plastic promoted the overall hydrolysis progression of anaerobic co-digestion, possibly as a result of the enhanced activities of Bacteroidota and Thermotogota. In conclusion, under anaerobic co-digestion, the modified the plastic not only achieved effective degradation but also facilitated the co-digestion process.

Oncolytic adenovirus in treating malignant ascites: A phase II trial and longitudinal single-cell study.

Malignant ascites is a common complication resulting from the peritoneal spread of malignancies, and currently lacks effective treatments. We conducted a phase II trial (NCT04771676) to investigate the efficacy and safety of oncolytic adenovirus H101 and virotherapy-induced immune response in 25 patients with malignant ascites. Oncolytic virotherapy achieved an increased median time to repeat paracentesis of 45 days (95% confidence interval 16.5-73.5 days), compared with the preset control value of 13 days. Therapy was well-tolerated, with pyrexia, fatigue, nausea, and abdominal pain as the most common toxicities. Longitudinal single-cell profiling identified marked oncolysis, early virus replication, and enhanced CD8+ T cells-macrophages immune checkpoint crosstalk, especially in responsive patients. H101 also triggered a proliferative burst of CXCR6+ and GZMK+CD8+ T cells with promoted tumor-specific cytotoxicity. Further establishment of oncolytic virus-induced T cell expansion signature (OiTE) implicated the potential benefits for H101-responsive patients from subsequent anti-PD(L)1 therapy. Patients with upregulated immune-signaling pathways in tumor cells and a higher proportion of CLEC10A+ dendritic cells and GZMK+CD8+ T cells at baseline showed a superior response to H101 treatment. Our study demonstrates promising clinical responses and tolerability of oncolytic adenovirus in treating malignant ascites and provides insights into the relevant cellular processes following oncolytic virotherapy.

An original aneuploidy-related gene model for predicting lung adenocarcinoma survival and guiding therapy.

Aneuploidy is a hallmark of cancers, but the role of aneuploidy-related genes in lung adenocarcinoma (LUAD) and their prognostic value remain elusive. Gene expression and copy number variation (CNV) data were enrolled from TCGA and GEO database. Consistency clustering analysis was performed for molecular cluster. Tumor microenvironment was assessed by the xCell and ESTIMATE algorithm. Limma package was used for selecting differentially expressed genes (DEGs). LASSO and stepwise multivariate Cox regression analysis were used to establish an aneuploidy-related riskscore (ARS) signature. GDSC database was conducted to predict drug sensitivity. A nomogram was designed by rms R package. TCGA-LUAD patients were stratified into 3 clusters based on CNV data. The C1 cluster displayed the optimal survival advantage and highest inflammatory infiltration. Based on integrated intersecting DEGs, we constructed a 6-gene ARS model, which showed effective prediction for patient's survival. Drug sensitivity test predicted possible sensitive drugs in two risk groups. Additionally, the nomogram exhibited great predictive clinical treatment benefits. We established a 6-gene aneuploidy-related signature that could effectively predict the survival and therapy for LUAD patients. Additionally, the ARS model and nomogram could offer guidance for the preoperative estimation and postoperative therapy of LUAD.

Using UV/peracetic acid as pretreatment for subsequent bio-treatment of antibiotic-containing wastewater treatment: Mitigating microbial inhibition and antibiotic resistance genes proliferation.

UV/peracetic acid (PAA) treatment presents a promising approach for antibiotic removal, but its effects on microbial community and proliferation of antibiotic resistance genes (ARGs) during the subsequent bio-treatment remain unclear. Thus, we evaluated the effects of the UV/PAA on tetracycline (TTC) degradation, followed by introduction of the treated wastewater into the bio-treatment system to monitor changes in ARG expression and biodegradability. Results demonstrated effective TTC elimination by the UV/PAA system, with carbon-centered radicals playing a significant role. Crucially, the UV/PAA system not only eliminated antibacterial activity but also inhibited potential ARG host growth, thereby minimizing the emergence and dissemination of ARGs during subsequent bio-treatment. Additionally, the UV/PAA system efficiently removed multi-antibiotic resistant bacteria and ARGs from the bio-treatment effluent, preventing ARGs from being released into the environment. Hence, we propose a multi-barrier strategy for treating antibiotic-containing wastewater, integrating UV/PAA pre-treatment and post-disinfection with bio-treatment. The inhibition of ARGs transmission by the integrated system was verified through actual soil testing, confirming its effectiveness in preventing ARGs dissemination in the surrounding natural ecosystem. Overall, the UV/PAA treatment system offers a promising solution for tackling ARGs challenges by controlling ARGs proliferation at the source and minimizing their release at the end of the treatment process.