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Tight ultra-filtration (TUF) membranes were constructed by in situ growing zinc imidazole frameworks micro-crystalline leaves (ZIF-L) in polyethylene imine (PEI) and polydopamine (PDA) deposit layers on porous polyethylene (PE) substrates. The effects of preparation conditions on the surface physical and chemical structures as well as on the dye/salt separation performance of the formed TUF membranes were systematically investigated. By inserting selective water permeation channels and increasing contacting surface areas, in situ-grown ZIF-L arrays tightly cross-linked in the coating matrix greatly increased water permeation without trading off dye/salt retention selectivity. The morphology of the included ZIF-L particles could be varied by adjusting the ligand/Zn molar ratio (α) in the preparation processes. Optimized PDA-PEI/ZIF-L@PE TUF membranes containing ZIF-L of cross-cross block morphology showed very high pure water permeability of 180 ± 20 L·m-2·h-1·bar-1 (LMHB) and retention selectivity (SCR/Na2SO4 and SMB/Na2SO4) of 267 and 43, respectively, as well as excellent stability and anti-fouling properties.
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Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/µL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.
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Purpose: To investigate the relationships between visual function and the retinal and choroid microstructure in idiopathic macular hole patients after surgery. Methods: A prospective study investigated changes in the fundus structure and visual function in 16 macular hole patients before and after surgery. Patients' best-corrected visual acuity (BCVA) and retinal sensitivity were measured by an EDTRS visual chart and microperimetry (MP1), respectively. The thickness of the retina and the blood supply to the retina and superficial choroid were detected by OCTA, and the choroidal capillary blood flow density was analysed with MATLAB. The thickness of the choroid and the aperture size of the macular hole were detected by Heidelberg OCT. Results: Compared with before surgery and one month after surgery, the BCVA (3 months: 0.47 ± 0.27, before: 1.02 ± 0.22, 1 month: 0.66 ± 0.27, and P < 0.05) and the central sensitivity of the retina (3 months: 14.88 ± 2.87 dB, before: 8.76 ± 3.27 dB, 1 month: 12.22 ± 3.30 dB, and P < 0.05) were significantly improved three months after surgery. The change in BCVA was significantly correlated with the basal diameter (r = 0.677 and P = 0.004), the minimum diameter (r = 0.585 and P = 0.017), the macular hole cystoid height area index (r = -0.618 and P = 0.011), the central macular hole index (r = -0.727 and P = 0.001), the peripheral macular hole index (r = -0.758 and P = 0.001), the central tractional hole index (r = -0.717 and P = 0.002), the peripheral tractional hole index (r = -0.725 and P = 0.001), and changes in the peripheral blood vessel density of the choroid capillary layer (r = 0.585 and P = 0.0017). The change in central retinal sensitivity was correlated with the change in the superficial foveal avascular zone (FAZ; r = 0.520 and P = 0.039), change in the retinal superficial peripheral blood flow density (r = -0.503 and P = 0.047), change in the deep FAZ (r = 0.599 and P = 0.014), and change in the retinal deep peripheral blood flow density (r = -0.601 and P = 0.014). Conclusions: The morphology of the macular hole as well as changes to the retinal and choroidal microstructure contributes to the recovery of visual function after surgery.
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A previous study by our group found that inhibition of nischarin promotes neurite outgrowth and neuronal regeneration in Neuro-2a cells and primary cortical neurons. In recent years, more and more studies have shown that nanomaterials have good prospects in treatment of spinal cord injury. We proposed that small interfering RNA targeting nischarin (Nis-siRNA) delivered by polyethyleneimine-alginate (PEI-ALG) nanoparticles promoted motor function recovery in rats with spinal cord injury. Direct microinjection of 5 µL PEI-ALG/Nis-siRNA into the spinal cord lesion area of spinal cord injury rats was performed. From day 7 after surgery, Basso, Beattie and Bresnahan score was significantly higher in rats from the PEI-ALG/Nis-siRNA group compared with the spinal cord injury group and PEI-ALG/Control-siRNA group. On day 21 after injection, hematoxylin-eosin staining showed that the necrotic area was reduced in the PEI-ALG/Nis-siRNA group. Immunohistochemistry and western blot assay results confirmed successful inhibition of nischarin expression and increased protein expression of growth-associated protein-43 in the PEI-ALG/Nis-siRNA group. These findings suggest that a complex of PEI-ALG nanoparticles and Nis-siRNA effectively suppresses nischarin expression, induces expression of growth-associated protein-43, and accelerates motor function recovery after spinal cord injury.
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OBJECTIVE: To study the effects of expressions of SCCA1 and SCCA2 in cervical squamous cell carcinoma on its diagnosis, treatment evaluation and prognosis analysis. M ethod s : Seventy-six cervical squamous cell carcinoma patients enrolled in our hospital from October 2011 to April 2013 were selected, and another 76 healthy females (without cervical tissue lesions) were enrolled as the control. SCCA1 and SCCA2 expressions in the two groups were compared by RT-PCR. The serodiagnosis results before and after chemotherapy were compared to clarify the effects of SCCA2 expression. RESULTS: The two groups had similar relative SCCA1 expression rates that were not significantly correlated with pathological factors. Before chemotherapy, the relative expression rates of SCCA2 were significantly higher in the patients with later stage (t=6.018, P=0.00082<0.05) and lymphatic metastasis (t=6.281, P=0.00192<0.05). After treatment, relative SCCA2 expression rate was decreased more significantly in the effective group than that in the ineffective group (t=10.27893, P=0.02815<0.05). CONCLUSION: The expression of SCCA1 failed to indicate the onset, diagnosis and prevention of cervical squamous cell carcinoma, whereas that of SCCA2 worked as one of the tumor markers.
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OBJECTIVE(S): To establish an experimental bowel endometriosis model in the rat and to investigate regional lymph node involvement. STUDY DESIGN: Twenty Sprague-Dawley rats were subjected to autologous transplantation of endometrial tissue to the ileocecum by laparotomy. After two months, endometriotic lesions and mesenteric lymph nodes were harvested by repeat laparotomy subjected to histologic assessment regarding the histologically verified presence of endometriosis in the ileocecum and in mesenteric lymph nodes. RESULT(S): A total of 20 rats were subjected to autologous transplantation of endometrial tissue to the ileocecum. Ileocecal endometriosis was successfully induced in 19/20 (95%) rats. In 18/20 animals, the mesenteric lymph node was identified and resected. Regional lymph node involvement by endometriosis was identified in 0/18 mesenteric lymph nodes. CONCLUSION(S): Endometriosis can be successfully induced in a rat model by autologous transplantation of endometrial tissue. In this experimental rodent endometriosis model, regional mesenteric lymph node involvement by endometriosis was not identified.