Long noncoding RNA SNHG8 promotes the proliferation of osteosarcoma cells by downregulating miR-542-3p.

Small nucleolar RNA host genes (SNHGs) as a subset of long noncoding RNAs (lncRNAs) have critical roles in the pathogenesis of multiple malignancies, however, the role and molecular mechanisms of lncRNA SNHG8 in osteosarcoma (OS) remain unclear. In the present study, the correlation of SNHG8 or miR-542-3p with clinicopathological elements and prognosis in OS patents was estimated by TCGA cohort. Cell viability and invasion were assessed by MTT and Transwell assays. The interplay between SNHG8 and miR-542-3p was affirmed by a luciferase report assay. The effects of SNHG8 on miR-542-3p expression were examined in MG-63 and SW-1353 cells by qRT-PCR analysis. The results showed that incremental expression of SNHG8 or reduced expression of miR-542-3p was related to poor survival and tumor recurrence in OS patients. Overexpressing SNHG8 accelerated the growth and invasion of MG-63 cells, but silencing SNHG8 harbored an opposite effect in SW-1353 cells. Additionally, SNHG8 could negatively regulate miR-542-3p expression and bind with miR-542-3p, which attenuated SNHG8 induced cell proliferation. Taken together, these findings indicate that lncRNA SNHG8 promotes the proliferation of OS cells by downregulating miR-542-3p.

Biomechanical assessment of a novel L4/5 level interspinous implant using three dimensional finite element analysis.

OBJECTIVES: Range of motion (ROM) is often restricted by conventional spinal fusion surgery, while some complications also occurred after applying posterior dynamic devices in clinic. Therefore, new surgical implant options were necessitated. The biomechanical features of a novel interspinous implant were investigated using three dimensional (3D) finite element models (FEMs). MATERIALS AND METHODS: An "H-shaped" polyether ether ketone (PEEK) interspinous implant was designed to tightly fit the upper and lower spinous processes, featuring a hollow cylindrical portion which was implanted autologous bones to enhance fusion with spinous processes. A 3D FEM of the intact L3/S segment with mild disc degeneration in L4/5 (degenerated model) was developed and subjected to flexion-extension, lateral bending, and axial rotation either with or without the implanted prosthesis (implant model) in order to examine effects on ROM, intradiscal stress, and facet joint load. RESULTS: The whole lumbar ROM was altered slightly by implant insertion, and reduced end plate stress, nucleus stress, and facet joints load at the L4/5 level (implant location) were observed. L4/5 flexion-extension maximal end plate stress, nucleus stress, and facet joints load were 5.262 MPa, 0.1648 MPa, and 29.7 N, respectively, in the degenerated model and 2.323 MPa, 0.0892 MPa, and 5.4 N, respectively, in the implant model. End plate and nucleus stresses were partially alleviated at the L3/4 level. Slightly higher maximal von Mises stress in L3/4 and L5/S annuli were observed in the implant model. CONCLUSIONS: The proposed novel interspinous implant effectively restored stability without producing excessive ROM limitations, meriting further clinical evaluation. Furthermore, these findings provide a useful basis for wide application of FEM in a broad variety of spinal implant assessments.