Electrospinning Aligned SF/Magnetic Nanoparticles-Blend Nanofiber Scaffolds for Inducing Skeletal Myoblast Alignment and Differentiation.

In the realm of skeletal muscle tissue engineering, anisotropic materials that emulate natural tissues show substantial promise. Electrospun scaffolds, mimicking the fibrillar structure of the extracellular matrix, are commonly employed but often fall short in achieving optimal alignment and mechanical strength. Silk fibroin has emerged as a versatile material in tissue engineering, valued for its biocompatibility, mechanical robustness, and biodegradability. However, conventional electrospinning methods of SF result in randomly oriented fibers, limiting their efficacy. In this work, we developed a straightforward method to fabricate directional tissue scaffolds using silk fibroin. By integrating a magnetic field collecting device and incorporating Fe3O4 nanoparticles into the spinning solution, we successfully produced well-aligned silk nanofiber scaffolds. These aligned fibers not only improved scaffold orientation and mechanical properties but also exhibited magnetic responsiveness. The aligned SF scaffolds effectively guided the adhesion, proliferation, and differentiation of mesenchymal stem cells along the fiber direction. Cultured on these scaffolds, myoblast C2C12 cells demonstrated oriented growth, highlighting the potential of aligned SF fibers in advancing skeletal muscle engineering for biomedical applications.

Design of Bombyx mori (B. mori) Silk Fibroin Microspheres for Developing Biosafe Sunscreen.

Sunscreens play a crucial role in protecting the skin from ultraviolet (UV) damage. However, present commercial sunscreens have a tendency to generate free radicals in the UV window, resulting in serious inflammatory responses and health problems. In this study, we demonstrate that silk fibroin microspheres (SFMPs) assembled from regenerated silk fibroin (SF) could scavenge free radicals while preventing UV irradiation and thus present a promising sunscreen. The SFMP reflected more UV light than SF and presented a higher stability than that of organic commercial sunscreens. In vitro analysis proved that SFMP could more efficiently scavenge the hydroxy radical and reduce the intracellular reactive oxygen than titanium dioxide (TiO2). In vivo experiments exhibited that SFMP provided stronger skin protection against UV irradiation than commercial sunscreens and TiO2. Furthermore, SFMP treatment significantly inhibited the skin inflammatory response. This work suggests that the SFMP has great potential to be developed into a biosafe sunscreen.

Alteration in Asymmetry of White Matter Network of Parkinson's Disease.

Parkinson's disease (PD) is manifest clinically by an asymmetrical presentation of motor dysfunction. A large number of previous neuroimaging research studies have stated the alteration in the hemispheric asymmetry of morphological features in PD disease. Diffusion Magnetic Resonance Imaging (MRI), which is noninvasive, has been widely used to quantify the white matter network in the human brain of both healthy subjects and patients. Besides, graph theory analysis is widely used to quantify the topological architecture of the human brain network. Lately, researchers have discovered that the topological architecture of the white matter network significantly differs in PD compared with healthy controls (HC). Nevertheless, the asymmetry of the topological architecture of the white matter network for PD patients remains unclear. To clarify this, the diffusion-weighted images and tractography technique were used to reconstruct the hemispherical white matter networks for 22 bilateral PD patients and 18 HC subjects. Network-based statistical analysis and graph theory analysis approaches were employed to estimate the asymmetry at both the connectivity level and the hemispheric topological level for PD patients. We found that the PD group showed atypically right-higher-than-left asymmetry in hemispheric brain global and local efficiencies. The detected right-higher-than-left asymmetry was driven by the atypically topological changes in the left hemispheric brain in the PD group. Findings from these studies might provide new insights into the asymmetric features of hemispheric disconnectivity and emphasize that the topological asymmetry of the hemispheric brain could be used as a biomarker to identify PD individuals.

Monitoring and Prognostic Analysis of Severe Cerebrovascular Diseases Based on Multi-Scale Dynamic Brain Imaging.

Severe cerebrovascular disease is an acute cerebrovascular event that causes severe neurological damage in patients, and is often accompanied by severe dysfunction of multiple systems such as breathing and circulation. Patients with severe cerebrovascular disease are in critical condition, have many complications, and are prone to deterioration of neurological function. Therefore, they need closer monitoring and treatment. The treatment strategy in the acute phase directly determines the prognosis of the patient. The case of this article selected 90 patients with severe cerebrovascular disease who were hospitalized in four wards of the Department of Neurology and the Department of Critical Care Medicine in a university hospital. The included cases were in accordance with the guidelines for the prevention and treatment of cerebrovascular diseases. Patients with cerebral infarction are given routine treatments such as improving cerebral circulation, protecting nutrient brain cells, dehydration, and anti-platelet; patients with cerebral hemorrhage are treated within the corresponding safe time window. We use Statistical Product and Service Solutions (SPSS) Statistics21 software to perform statistical analysis on the results. Based on the study of the feature extraction process of convolutional neural network, according to the hierarchical principle of convolutional neural network, a backbone neural network MF (Multi-Features)-Dense Net that can realize the fusion, and extraction of multi-scale features is designed. The network combines the characteristics of densely connected network and feature pyramid network structure, and combines strong feature extraction ability, high robustness and relatively small parameter amount. An end-to-end monitoring algorithm for severe cerebrovascular diseases based on MF-Dense Net is proposed. In the experiment, the algorithm showed high monitoring accuracy, and at the same time reached the speed of real-time monitoring on the experimental platform. An improved spatial pyramid pooling structure is designed to strengthen the network's ability to merge and extract local features at the same level and at multiple scales, which can further improve the accuracy of algorithm monitoring by paying a small amount of additional computational cost. At the same time, a method is designed to strengthen the use of low-level features by improving the network structure, which improves the algorithm's monitoring performance on small-scale severe cerebrovascular diseases. For patients with severe cerebrovascular disease in general, APACHEII1, APACHEII2, APACHEII3 and the trend of APACHEII score change are divided into high-risk group and low-risk group. The overall severe cerebrovascular disease, severe cerebral hemorrhage and severe cerebral infarction are analyzed, respectively. The differences are statistically significant.

Preparation of Z-scheme AgI/Bi5O7I plate with high visible light photocatalytic performance by phase transition and morphological transformation of BiOI microspheres at room temperature.

AgI/Bi5O7I plate composite was fabricated at room temperature by the phase transition and morphological transformation of BiOI microspheres. The orange BiOI microsphere was transferred to light yellow AgI/Bi5O7I plate by the addition of NaOH and AgNO3 to the system. The formation process and possible transformation mechanism of AgI/Bi5O7I plate were detected and are discussed. The visible light absorption area of AgI/Bi5O7I extended to 667 nm and photo-excited electrons migrated from Bi5O7I to AgI to effectively inhibit the recombination of photo-generated carriers in the Z-scheme AgI/Bi5O7I plate. As a result, the photocatalytic ability of AgI/Bi5O7I plate increased by a factor of 21.9 than that of pure Bi5O7I and a factor of 45.7 than that of pure AgI under visible light irradiation. This study provides a facile fabrication of Z-scheme visible light photocatalysts with high performance and a method for designing semiconductor photocatalysts with controllable morphology.