RESUMO
Biochar has been used to enhance methane generation from anaerobic digestion through establishing direct interspecific electron transfer between microorganisms. However, the microbial communication is still inadequate, thereby limiting further methane production improvement contributed by biochar. This study investigated the roles of quorum-sensing molecules, acylated homoserine lactone (AHL), in anaerobic digestion of waste activated sludge aided by biochar. Results showed that the co-addition of separated biochar and AHL achieved best methane production performance, with the maximal methane yield of 154.7 mL/g volatile suspended solids, which increased by 51.9%, 47.2%, 17.9%, and 39.4% respectively compared to that of control, AHL-loaded biochar, sole AHL, and sole biochar groups. The reason was that the co-addition of separated biochar and AHL promoted the stages of hydrolysis and acidification, promoting the conversion of organic matters and short-chain fatty acids, and optimizing the accumulation of acetate acid. Moreover, the methanogenesis stage also performed best among experimental groups. Correspondingly, the highest activities of electron transfer and coenzyme F420 were obtained, with increase ratios of 33.2% and 27.2% respectively compared to that of control. Furthermore, biochar did more significant effects on the evolution of microbial communities than AHL, and the direct interspecific electron transfer between fermentative bacteria and methanogens were possibly promoted.
Assuntos
Carvão Vegetal , Metano , Percepção de Quorum , Metano/metabolismo , Anaerobiose , Esgotos , Ácidos Graxos Voláteis/metabolismo , Acil-Butirolactonas/metabolismoRESUMO
To recover methane from waste activated sludge through anaerobic digestion (AD) is one promising alternative to achieve carbon neutrality for wastewater treatment plants. However, humic acids (HAs) are one of the major compositions in waste activated sludge, and their accumulation performs inhibition effects on AD. This study investigated the potentials of biochar (BC) in alleviating inhibition effects of HAs on AD. Results showed that although the accumulated HAs reduced methane yield by 9.37% compared to control, the highest methane yield, 132.6 mL CH4/g VSS, was obtained after adding BC, which was 45.9% higher than that in HA group. Mechanism analysis showed that BC promoted the activities of hydrolase such as protease and α-glucosidase, which were 69.7% and 29.7% higher than those in HA group, respectively. The conversion of short-chain fatty acids was accelerated. In addition, the evolutions of electroactive microorganisms like Clostridium_sensu_stricto_13 and Methanosaeta were consistent with the activitiies of electron transfer and the contents of cytochrome c. Furthermore, parts of HAs rather than all of them were adsorbed by BC, and the remaining free HAs and BC formed synergistic effects on methanogenesis, then both CO2 reduction and acetoclastic methanogenesis pathways were improved. The findings may provide some solutions to alleviate inhibition effects of HAs on AD.
Assuntos
Carvão Vegetal , Substâncias Húmicas , Metano , Carvão Vegetal/química , Carvão Vegetal/farmacologia , Anaerobiose , Metano/metabolismo , Esgotos/microbiologia , Eliminação de Resíduos Líquidos/métodos , Reatores BiológicosRESUMO
Biochar produced from bio-wastes has been widely used to promote the performance of anaerobic digestion. Waste activated sludge (WAS) is considered as a kind of popular precursor for biochar preparation, but the abundant resources in WAS were neglected previously. In this study, the roles of biochar prepared from raw, pretreated, and fermented sludge on anaerobic digestion were investigated. That is, parts of carbon sources and nutrients like polysaccharides, proteins, and phosphorus were firstly recovered after sludge pretreatment or fermentation, and then the sludge residuals were used as raw material to prepare biochar. The methane yield improved by 22.1% with adding the biochar (AK-BC) prepared by sludge residual obtained from alkaline pretreatment. Mechanism study suggested that the characteristics of AK-BC like specific surface area and defect levels were updated. Then, the conversion performance of intermediate metabolites and electro-activities of extracellular polymeric substances were up-regulated. As a result, the activity of electron transfer was increased with the presence of AK-BC, with increase ratio of 21.4%. In addition, the electroactive microorganisms like Anaerolineaceae and Methanosaeta were enriched with the presence of AK-BC, and the potential direct interspecies electron transfer was possibly established. Moreover, both aceticlastic and CO2-reducing methanogenesis pathways were improved by up-regulating related enzymes. Therefore, the proposed strategy can not only obtain preferred biochar but also recover abundant resources like carbon source, nutrients, and bioenergy.
Assuntos
Carvão Vegetal , Metano , Esgotos , Carvão Vegetal/química , Esgotos/química , Esgotos/microbiologia , Anaerobiose , Metano/metabolismo , Eliminação de Resíduos Líquidos/métodos , Álcalis/química , Reatores BiológicosRESUMO
Potassium ferrate (K2FeO4) has been extensively employed to promote short-chain fatty acids (SCFAs) production from anaerobic fermentation of waste activated sludge (WAS) because of its potent oxidizing property and formation of alkaline hydrolyzed products (potassium hydroxide, KOH and ferric hydroxide, Fe(OH)3). However, whether K2FeO4 actually works as dual functions of both an oxidizing agent and an alkalinity enhancer during the anaerobic fermentation process remains uncertain. This study aims to identify the contributions of hydrolyzed products of K2FeO4 on SCFAs production. The results showed that K2FeO4 did not execute dual functions of oxidization and alkalinity in promoting SCFAs production. The accumulation of SCFAs using K2FeO4 treatment (183 mg COD/g volatile suspended solids, VSS) was less than that using either KOH (192 mg COD/g VSS) or KOH & Fe(OH)3 (210 mg COD/g VSS). The mechanism analysis indicated that the synergistic effects caused by oxidization and alkalinity properties of K2FeO4 did not happen on solubilization, hydrolysis, and acidogenesis stages, and the inhibition effect caused by K2FeO4 on methanogenesis stage at the initial phase was more severe than that of its hydrolyzed products. It was also noted that the inhibition effects of K2FeO4 and its hydrolyzed products on the methanogenesis stage could be relieved during a longer sludge retention time, and the final methane yields using KOH or KOH & Fe(OH)3 treatment were higher than that using K2FeO4, further confirming that dual functions of K2FeO4 were not obtained. Therefore, K2FeO4 may not be an alternative strategy for enhancing the production of SCFAs from WAS compared to its alkaline hydrolyzed products. Regarding the strong oxidization property of K2FeO4, more attention could be turned to the fates of refractory organics in the anaerobic fermentation of WAS.
Assuntos
Compostos de Potássio , Esgotos , Ácidos Graxos VoláteisRESUMO
Anaerobic digestion is widely employed for the treatment of waste activated sludge (WAS) due to its advantages like simultaneous energy recovery and sludge stabilization, promoting carbon-neutral operation of wastewater treatment plants. Natural zeolite, a low-cost and eco-friendly additive, has the potential to improve methane production from anaerobic digestion. This study investigated the effects of natural zeolite on anaerobic digestion when the substrate was WAS. It was found that methane production potential in response to natural zeolite was dosage-dependent. The optimal dosage was 0.1 g zeolite/g volatile suspended solids (VSS), with a methane yield of 181.89 ± 6.75 mL/g VSS, which increased by 20.1% compared to that of the control. Although the methane yields with other dosages of natural zeolite were higher than that of control, they were lesser than that with 0.1 g zeolite/g VSS. Natural zeolite affected transfer and conversion of proteins much more than polysaccharides in liquid phase and extracellular polymeric substances. In anaerobic digestion, natural zeolite had with little effects on WAS solubilization, while it improved hydrolysis, acidification, and methanogenesis. The dosages of natural zeolite did have significant effects on bacterial communities in biofilm rather than suspension, while the archaeal communities in biofilm and suspension were all greatly related to natural zeolite dosages. The developed biofilms promoted richness and functionality of microbial communities. The syntrophic metabolism relationships between methanogens and bacteria were improved, which was proved by selective enrichment of Methanosarcina, Syntrophomonas, and Petrimonas. The findings of this work provided some new solutions for promoting methane production from WAS, and the roles of natural zeolite in anaerobic digestion.
Assuntos
Esgotos , Zeolitas , Esgotos/química , Anaerobiose , Eliminação de Resíduos Líquidos , Bactérias/metabolismo , Metano , Biofilmes , Reatores BiológicosRESUMO
Anaerobic digestion has been proved as one promising strategy to simultaneously achieve resource recovery and environmental pollution control for biosolid treatment, and adding exogenous materials is a potential alternative to promote the above process. This study investigated response mechanisms of anaerobic digestion of waste activated sludge (WAS) to particle sizes of zeolite. Results showed that the methane production reached 186.75 ± 7.62 mL/g volatile suspended solids (VSS) with zeolite of the particle size of 0.2-0.5 mm and the additive dosage of 0.1 g/g VSS, which increased by 22.08% compared to that in control. Mechanism study revealed that zeolite could improve hydrolysis, acidification, and methanogenesis stages. Rapid consumption rates of soluble polysaccharides and proteins were observed, correspondingly, the accumulations of short-chain fatty acids (SCFAs) were enhanced, and the compositions of SCFAs were optimized. Moreover, the activities of F420 increased by 28% with zeolite, and the syntrophic metabolism between bacteria and methanogens were promoted.
Assuntos
Esgotos , Zeolitas , Esgotos/microbiologia , Anaerobiose , Tamanho da Partícula , Reatores Biológicos/microbiologia , Ácidos Graxos Voláteis/metabolismo , Metano/metabolismo , Eliminação de Resíduos Líquidos/métodosRESUMO
Methane is one of the most promising renewable energies to alleviate energy crisis or replace fossil fuels, which can be recovered from anaerobic digestion of bio-wastes. However, the engineering application of anaerobic digestion is always hindered by low methane yield and production rate. This study revealed the roles and mechanisms of a green-prepared magnetic biochar (MBC) in promoting methane production performance from waste activated sludge. Results showed that the methane yield reached 208.7 mL/g volatile suspended solids with MBC additive dosage of 1 g/L, increasing by 22.1 % compared to that in control. Mechanism analysis demonstrated that MBC could promote hydrolysis, acidification, and methanogenesis stages. This was because the properties of biochar were upgraded by loading nano-magnetite, such as specific surface area, surface active sites, and surface functional groups, which made MBC have greater potential to mediate electron transfer. Correspondingly, the activity of α-glucosidase and protease respectively increased by 41.7 % and 50.0 %, and then the hydrolysis performances of polysaccharides and proteins were improved. Also, MBC improved the secretion of electroactive substances like humic substances and cytochrome C, which could promote extracellular electron transfer. Furthermore, Clostridium and Methanosarcina, as well-known electroactive microbes, were selectively enriched. The direct interspecies electron transfer between them was established via MBC. This study provided some scientific evidences to comprehensively understand the roles of MBC in anaerobic digestion, with important implications for achieving resource recovery and sludge stabilization.
Assuntos
Reatores Biológicos , Esgotos , Anaerobiose , Esgotos/química , Metano/metabolismo , Fenômenos MagnéticosRESUMO
Conductive materials have been widely used to establish direct interspecies electron transfer (DIET) for enhancing methane production potential from anaerobic digestion of waste activated sludge (WAS). However, the roles of extracellular polymeric substances (EPSs) affected by conductive materials on anaerobic digestion have been rarely reported. This study selected four widely used conductive materials, i.e., granular active carbon (GAC), biochar (BC), zero-valent iron (ZVI), and magnetite (Mag), to reveal the roles of EPSs. Results showed that methane production potentials were increased by BC, ZVI and Mag compared to that of control, with increase ratios of 13.4 %, 22.2 %, and 12.2 %, while a decrease was observed by GAC. The contents, components and characteristics of EPSs were all affected by conductive materials. The contents of EPSs were increased by ZVI and Mag, while they were decreased by BC and GAC. The ratios between proteins and polysaccharides (PN/PS) in loosely bound EPSs (LB-EPSs) were reduced in all groups, while they were similar in tightly bound EPSs (TB-EPSs) of ZVI and Mag groups. In addition, the cytochrome C and redox properties were remarkably promoted in suspension rather than in LB- and TB-EPSs. It was found that the correlation relationships between the maximal methane production potential (Pmax) and PN/PS in EPSs were positive, as well as fluorescent substances, especially tyrosine-like and tryptophan-like substances, with R2 of 0.96 and 0.98. Furthermore, the correlation relationships also existed between EPSs and microbial communities. Clostridium and Methanobacterium, potential DIET partners, presented significant positive correlation relationships (P < 0.05) with Pmax, PN/PS and fluorescent substances in EPSs. The findings may provide some new insights for mechanism investigation of anaerobic digestion induced by conductive materials.
Assuntos
Matriz Extracelular de Substâncias Poliméricas , Esgotos , Esgotos/microbiologia , Matriz Extracelular de Substâncias Poliméricas/metabolismo , Óxido Ferroso-Férrico , Citocromos c/metabolismo , Triptofano , Ferro , Polissacarídeos/metabolismo , Metano/metabolismo , Tirosina/metabolismo , Carbono , Anaerobiose , Reatores BiológicosRESUMO
With the rapid growth of population and urbanization, more and more bio-wastes have been produced. Considering organics contained in bio-wastes, to recover resource from bio-wastes is of great significance, which can not only achieve the resource recycle, but also protect the environment. Anaerobic digestion (AD) has been proved as one of the most promising strategies to recover bio-energy from bio-wastes, as well as to realize the reduction of bio-wastes. However, the conventional interspecies electron transfer is sensitive to environmental shocks, such as high ammonia, organic pollutants, metal ions, etc., which lead to instability or failure of AD. The recent findings have proved that the introduction of zero-valent iron (ZVI) in AD system can significantly enhance methane production from bio-wastes. This review systematically highlighted the recent advances on the roles of ZVI in AD, including underlying mechanisms of ZVI on AD, performance enhancement of AD contributed by ZVI, and impact factors of AD regulated by ZVI. Furthermore, current limitations and outlooks have been analyzed and concluded. The roles of ZVI on underlying mechanisms in AD include regulating reaction conditions, electron transfer mode and function of microbial communities. The addition of ZVI in AD can not only enhance bio-energy recovery and toxic contaminants removal from bio-wastes, but also have the potential to buffer adverse effect caused by inhibitors. Moreover, the electron transfer modes induced by ZVI include both interspecies hydrogen transfer and direct interspecies electron transfer pathways. How to comprehensively evaluate the effects of ZVI on AD and further improve the roles of ZVI in AD is urgently needed for practical application of ZVI in AD. This review aims to provide some references for the introduction of ZVI in AD for enhancing bio-energy recovery from bio-wastes.
Assuntos
Poluentes Ambientais , Ferro , Anaerobiose , Amônia , Metano , HidrogênioRESUMO
Alkaline pretreatment is one promising strategy for promoting anaerobic digestion of waste activated sludge (WAS). This study selected three types of alkalis with monovalent (NaOH and KOH), divalent (Ca(OH)2 and Mg(OH)2), and trivalent (Fe(OH)3 and Al(OH)3) cations to reveal the roles of metal ions on short chain fatty acids (SCFAs) production. The enhanced production potentials of SCFAs were reduced by order of alkalis with monovalent, divalent, and trivalent cations. Na+, K+, Ca2+, and Mg2+ did no contributions on SCFAs production, while Fe3+ and Al3+ performed better than control, especially the latter. The mechanism analysis proved that Na+, K+, Ca2+, and Mg2+ did no significant effects on solubilization, hydrolysis, acidification and methanogenesis stages, while the first three stages were improved by Fe3+ and Al3+ and the methanogenesis stage was inhibited. The findings may provide some new insights when using alkalis or residual metal ions to improve anaerobic digestion of WAS.
Assuntos
Álcalis , Esgotos , Anaerobiose , Cátions , Ácidos Graxos Voláteis , Metais , Hidróxido de SódioRESUMO
Waste activated sludge (WAS) is a potential resource to achieve carbon-neutral goal of wastewater treatment plant. However, the solubilization is always the rate-limiting step for resource recovery from anaerobic digestion of WAS. This study reported a novel strategy, i.e., potassium ferrate (PF) followed by alkali-stripping treatment, to achieve short-chain fatty acids (SCFAs) and nitrogen recovery from WAS. Results showed that whether the stripping process was conducted under alkaline condition or not, the SCFAs production potential was increased rather than reduced. The promoted SCFAs production was due to the accelerated solubilization and hydrolysis stages but the inhibited methanogenesis stage. The SCFAs yield reached 258 mg chemical oxygen demand (COD)/g volatile suspended solids (VSS), and the carbon source, including SCFAs, soluble polysaccharides and proteins, reached 384 mg COD/g VSS. The potentially recovered nitrogen was about 8.71 mg NH4+-N/g VSS. This work may provide some new solutions for enhancing resource recovery from WAS.
Assuntos
Nitrogênio , Esgotos , Álcalis , Anaerobiose , Carbono , Ácidos Graxos Voláteis , Fermentação , Concentração de Íons de Hidrogênio , Compostos de Ferro , Compostos de PotássioRESUMO
Methane recovered from anaerobic digestion of waste activated sludge (WAS) can be used as the energy supplement of the wastewater treatment plant, benefiting to its carbon-neutral operation. In order to enhance methane production, biochar (BC) has been widely selected as conductive material to build direct interspecies electron transfer (DIET) in anaerobic digestion of WAS. However, the role and significance of washing strategies, including water and acid washing, on BCs for regulating methane production have not been reported. This study selected the frequently used woody- (W) and straw (S)-BCs as mode. Compared to raw W-BC, water and acid washing W-BC increased the methane yields by 19.1% and 15.7%, respectively. Differently, the methane yields among raw, water and acid washing S-BCs were similar. Mechanism study showed that both the two washing strategies optimized the properties of raw W-BC for promoting methane production. Water and acid washing W-BCs increased the electron transfer functional groups, such as ketones and quinones, which were not observed in S-BCs. Moreover, the electron-active microorganisms were enriched with the presence of water and acid washing W-BCs, and the predominant pathway for methane production shifted from hydrogentrophic to acetotrophic and DIET methanogenesis, while the microbial communities, including bacteria and archaea, were similar with the presence of raw, water and acid washing S-BCs. These findings of this work provide some new insights for production improvement regulation of methane from anaerobic digestion of wastes induced by BCs.
Assuntos
Reatores Biológicos , Esgotos , Anaerobiose , Reatores Biológicos/microbiologia , Carvão Vegetal , Metano , Esgotos/microbiologia , Águas Residuárias , ÁguaRESUMO
This study proposed a novel and high-efficiency strategy, i.e., freezing followed by low-temperature thermal treatment, to significantly promote short-chain fatty acids (SCFAs) production from waste activated sludge compared to traditional freezing/thawing treatment. The maximal production of SCFAs was 212 mg COD/g VSS with a shortened retention time of five days, and the potentially recovered carbon source, including SCFAs, soluble polysaccharides and proteins, reached 321 mg COD/g VSS, increased by 92.1 and 28.3% compared to sole freezing and thermal treatment. Both the solubilization and hydrolysis steps of WAS were accelerated, and the acid-producing microorganisms, such as Macellibacteroides, Romboutsia and Paraclostridium, were greatly enriched, with a total abundance of 13.9%, which was only 0.54% in control. Interestingly, the methane production was inhibited at a shortened retention time, resulting in SCFAs accumulation, whereas it was increased by 32.0% at a longer sludge retention time, providing a potential solution for energy recovery from WAS.
Assuntos
Ácidos Graxos Voláteis , Esgotos , Anaerobiose , Fermentação , Congelamento , Concentração de Íons de Hidrogênio , TemperaturaRESUMO
Waste activated sludge (WAS), as the byproducts of wastewater treatment plants, has been greatly produced. With high cost and environmental risk of WAS disposal, to explore a low-cost and environment-friendly technology has been a great challenge. Considering that WAS is a collection of organic matters, anaerobic fermentation has been selected as a sustainable way to simultaneously recover resources and reduce environmental pollution. To recover short-chain fatty acids (SCFAs) has gained great concern because of the high value-added application and high-efficiency production process. Considering the temperature in some areas of the world can reach to below 0 °C, this study proposed an efficient strategy, i.e., stepwise freezing and thawing treatment, to promote SCFAs production. The maximal production of SCFAs, i.e., 246 mg COD/g volatile suspended solid, was obtained with the shortened retention time of five days. Mechanistic studies showed that the solubilization of both extracellular polymeric substances (EPSs) and microbial cells could be accelerated, with the EPSs removal of 58.3% for proteins and 59.0% for polysaccharides. Also, the hydrolysis process was promoted to provide more substrates for subsequent acidogenisis, and the functional microorganisms, such as Romboutsia, Paraclostridium, Macellibacteroides and Conexibacter, were greatly enriched, with a total abundance of 26.2%. Moreover, compared to control, methanogenesis was inhibited at a shortened sludge retention time (e.g., five days), which benefited to the accumulation of SCFAs, but the methane production was increased by 25.2% at a longer sludge retention time (e.g., ten days). Thus, these findings of this work may provide some new solutions for the enhanced resource recovery from WAS, and further for carbon-neutral operation of wastewater treatment plants.
Assuntos
Ácidos Graxos Voláteis , Esgotos , Anaerobiose , Matriz Extracelular de Substâncias Poliméricas , Fermentação , Congelamento , Concentração de Íons de Hidrogênio , Hidrólise , Esgotos/químicaRESUMO
To recover resource from waste activated sludge (WAS) is of great significance. This study proposed a promising way, i.e., stepwise alkaline treatment coupled with ammonia stripping, to remarkably enhance short-chain fatty acids (SCFAs) production from WAS anaerobic digestion. The maximal production of SCFAs, with the value of 323 mg COD/g volatile suspended solid, was obtained with first initial pH = 10 adjustment followed by second initial pH = 10 adjustment on third day coupled with ammonia stripping. Mechanistic studies showed that solubilization of both extracellular polymeric substances and cells could be accelerated by stepwise initial pH = 10 adjustment. However, without ammonia stripping, the activities of either acidogens or methanogens could be inhibited by free ammonia formed under alkaline conditions; positively, anaerobes related to SCFAs production were enriched with ammonia stripping. Moreover, the proposed strategy can simultaneously achieve nitrogen and carbon recovery, providing some solutions for the carbon-neutral operation of wastewater treatment plants.
Assuntos
Amônia , Esgotos , Ácidos Graxos Voláteis , Fermentação , Concentração de Íons de HidrogênioRESUMO
Quaternary ammonium compounds have gained widespread attention due to their extensive enrichment in waste activated sludge (WAS) and potentially adverse effect to anaerobes. This study selected benzalkonium chlorides (BACs) as model to reveal the responses of anaerobic digestion of WAS to long-term stress of BACs. Results showed that the solubilization enhancement of WAS contributed by BACs was the acceleration of cell lysis, rather than the disruption of extracellular polymeric substances, and the accumulation improvement of short chain fatty acids (SCFAs) attributed to hydrolysis improvement and methanogenesis inhibition at either medium -or high level of BACs. In addition, a low level had no significant effect on the production of methane compared to control, with averages of 0.059 and 0.055 m3/(m3·d), respectively, whereas a medium level reduced methane production to 20% of control, and a high level almost completely inhibited methanogenesis. Correspondingly, BACs could shift microbial communities related to SCFAs and methane productions. For the bacterial community, a high level of BACs led to abundance reductions of Firmicutes, Bacteroidetes, Acidobacteria and Chloroflexi, but Synergistetes was increased to 10.5%, which was almost not detected either in control or at a low level of BACs. And for dominant archaeal community, they tended to be shifted from acetotrophic to hydrogenotrophic methanogens with BACs increasing from low to high level. These findings provided some new insights for the role of BACs in anaerobic digestion, as well as resource recovery from WAS.
Assuntos
Microbiota , Esgotos , Anaerobiose , Compostos de Benzalcônio , Reatores Biológicos , Cloretos , Matriz Extracelular de Substâncias Poliméricas , Metano , Eliminação de Resíduos LíquidosRESUMO
The plastic products have large consumption over last decades, resulting in a serious microplastics (MPs) pollution. Specially, the main removal way of MPs from wastewater is to transfer MPs from liquid to solid phase, leading to its enrichment in waste activated sludge (WAS). Anaerobic digestion has been served as the most potential technique to achieve both resource recovery and sludge reduction, herein this review provides current information on occurrence, effect, and fate of MPs in anaerobic digestion of WAS. The effects of MPs on WAS anaerobic digestion are greatly related to forms, particles sizes, contents, compositions and leachates of MPs. Also, the presence of MPs not only can change the effects of other pollutants on anaerobic digestion of WAS, but also can affect the fates of them. Besides, the future perspectives focused on the fate, effect and final removal of MPs during WAS anaerobic digestion process are outlined.
Assuntos
Microplásticos , Esgotos , Anaerobiose , Plásticos , Eliminação de Resíduos Líquidos , Águas ResiduáriasRESUMO
This study was conducted to investigate the effects of residual ferric ions (FI), released from iron or its oxides for wastewater or waste activated sludge (WAS) treatment, on anaerobic digestion of WAS. Herein it was found that the anaerobic digestion process was greatly affected by FI dosages as well as FI distributions. The responses of performance and microorganism suggested that a low FI (e.g., 0.125 mmol/g volatile suspended solid (VSS)) enhanced methane production by 29.3%, and a medium FI (e.g., 0.3 mmol/g VSS) promoted short chain fatty acids accumulation to reach the maximum of 247 mg chemical oxygen demand /g VSS, conversely, a high FI (e.g., 0.9 mmol/g VSS) led to severe inhibition on acidogenesis and methanogenesis. The findings may provide some new insights for mechanism understanding on anaerobic digestion process influenced by iron or its oxides, as well as the disposal of WAS contained FI.