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Anammox is recognized as a prospective alternative for future biological nitrogen removal technologies. However, the nitrate by-products produced by anammox bacteria limit its overall nitrogen removal efficiency below 88 %. This study introduced Fe(III) into the anammox bioreactor to enhance the nitrogen removal efficiency to approximately 95 %, surpassing the biochemical limit of 88 % imposed by anammox stoichiometry. Anammox sludge was demonstrated to utilize extracellular polymeric substances to reduce Fe(III) into Fe(II), and this process promoted the dominance of Ca. Brocadia. The iron addition improved the abundance of narGHI genes and facilitated the partial dissimilatory nitrate reduction to ammonium, with nitrite as the end product. The accumulated nitrite was then eliminated through the anammox pathway, along with the excess ammonium (30 mg/L) in the influent. Overall, this study deepens our understanding of the enhanced nitrogen removal triggered by Fe(III) in anammox sludge and offers an effective approach to boost anammox process.
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Reatores Biológicos , Nitratos , Nitrogênio , Oxirredução , Esgotos , Nitrogênio/metabolismo , Nitratos/metabolismo , Esgotos/microbiologia , Compostos de Amônio/metabolismo , Compostos Férricos/metabolismo , Anaerobiose , Ferro/metabolismo , Bactérias/metabolismoRESUMO
Electrochemical desalination is an effective method for recovering salts from reverse osmosis (RO) brine. However, traditional technologies like bipolar membrane technology often face challenges related to membrane blockage. To overcome this issue, a preparative vertical-flow electrophoresis (PVFE) system was used for the first time to treat RO brine of petrochemical wastewater. In order to optimize the PVFE operation and maximize acids and bases production while minimizing energy consumption, the response surface method was employed. The independent variables selected were the electric field intensity (E) and flow rate (v), while the dependent variables were the acid-base concentration and energy consumption (EC) for acid-base production. Using the central composite design methodology, the operation parameters were optimized to be E = 154.311 V/m and v = 0.83 mL/min. Under these conditions, the base concentrations of the produced bases and acids reached 3183.06 and 2231.63 mg/L, respectively. The corresponding base EC and acid EC were calculated to be 12.57 and 11.62 kW·h/kg. In terms of the acid-base concentration and energy consumption during the PVFE process, the electric field intensity was found to have a greater influence than the flow rate. These findings provide a practical and targeted solution for recycling waste salt resources from RO brine.
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Osmose , Águas Residuárias , Águas Residuárias/química , Eletroforese , Eliminação de Resíduos Líquidos/métodos , SaisRESUMO
Usually, CymA is irreplaceable as the electron transport hub in Shewanella oneidensis MR-1 bidirectional electron transfer. In this work, biologically self-assembled FeS nanoparticles construct an artificial electron transfer route and implement electron transfer from extracellular into periplasmic space without CymA involvement, which present similar properties to type IV pili. Bacteria are wired up into a network, and more electron transfer conduits are activated by self-assembled transmembrane FeS nanoparticles (electron conduits), thereby substantially enhancing the ammonia production. In this study, we achieved an average NH4+-N production rate of 391.8 µg·h-1·L reactor-1 with the selectivity of 98.0% and cathode efficiency of 65.4%. Additionally, the amide group in the protein-like substances located in the outer membrane was first found to be able to transfer electrons from extracellular into intracellular with c-type cytochromes. Our work provides a new viewpoint that contributes to a better understanding of the interconnections between semiconductor materials and bacteria and inspires the exploration of new electron transfer chain components.
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Amônia , Shewanella , Amônia/metabolismo , Transporte de Elétrons , Shewanella/metabolismo , Elétrons , Eletrodos , Fontes de Energia BioelétricaRESUMO
Some nano-biochars (nano-BCs) as electron mediators could enter into cells to directly promote intracellular electron transfer and cell activities. However, little information was available on the effect of nano-BCs on SMX degradation. In this study, nano-BCs were prepared using sludge-derived humic acid (SHA) and their effects on SMX degradation by Shewanella oneidensis MR-1 were investigated. Results showed that nano-BCs (Carbon dots, CDs, <10 nm) synthesized using SHA performed a better accelerating effect than that of the nano-BCs with a larger size (10-100 nm), which could be attributed to the better electron transfer abilities of CDs. The degradation rate of 10 mg/L SMX in the presence of 100 mg/L CDs was significantly increased by 84.6% compared to that without CDs. Further analysis showed that CDs could not only be combined with extracellular Fe(III) to accelerate its reduction, but also participate in the reduction of 4-aminobenzenesulphonic acid as an intermediate metabolite of SMX via coupling with extracellular Fe(III) reduction. Meanwhile, CDs could enter cells to directly participate in intracellular electron transfer, resulting in 32.2% and 25.2% increases of electron transfer system activity and ATP level, respectively. Moreover, the activities of SMX-degrading enzymes located in periplasm and cytoplasm were increased by around 2.2-fold in the presence of CDs. These results provide an insight into the accelerating effect of nano-BCs with the size of <10 nm on SMX degradation and an approach for SHA utilization.
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Substâncias Húmicas , Esgotos , Shewanella , Sulfametoxazol , Shewanella/metabolismo , Esgotos/microbiologia , Sulfametoxazol/metabolismo , Anaerobiose , Biodegradação AmbientalRESUMO
Although polymeric anion exchange resins can remove phosphonates, they lack selectivity for target phosphonates and are susceptible to interference by anions and other substances. Here, we developed a novel strategy via confining MIL-101(Fe)-NH2 inside commercial resins IRA-900 for high-efficient and precise phosphonate removal, accompanying with the improvement of the stability and recovery of MIL-101(Fe)-NH2. The obtained nanocomposite MIL-101(Fe)-NH2@IRA-900 (MFNI) exhibited significantly enhanced phosphonate removal in the presence of competing anions (Cl-, SO42-, NO3- and CO32-) and natural organic matter (humic acid) at high concentrations (2-4 times of phosphonate concentration). Moreover, MFNI displayed the highest phosphonate adsorption capacity (12.9 mg P/g) and the fastest adsorption kinetics (120 min) than hydrated ferric oxides modified IRA-900 (HFOI) (6.7 mg P/g, 180 min), MIL-101(Fe)-NH2 (7.6 mg P/g, 240 min) and IRA-900 (5.6 mg P/g, 360 min). Such higher adsorption affinity and anti-interference ability came from the synergistic effect of the host IRA-900 (hydrogen-bond interaction and electrostatic attraction) and the embedded MIL-101(Fe)-NH2 (ligand exchange). The depleted MFNI could be regenerated with a binary NaOH-NaCl solution and reused without significant loss of capacity. Column adsorption runs by using MFNI indicated the fresh MFNI could achieve 100 % removal of PPOA in 10.5 h continuously feeding, which offered the possibility of achieving potential large-scale applications. In general, a new MOF-confined design approach was practiced to achieve selective elimination of phosphates and to improve the stability and recovery of MOF.
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Bulk carbon-based materials can enhance anaerobic biodenitrification when they are present in extracellular matrices. However, little information is available on the effect of nitrogen and iron co-doped carbon dots (N, Fe-CDs) with sizes below 10 nm on this process. This work demonstrated that Fe-NX formed in N, Fe-CDs and their low surface potentials facilitated electron transfer. N, Fe-CDs exhibited good biocompatibility and were effectively absorbed by Pseudomonas stutzeri ATCC 17588. Intracellular N, Fe-CDs played a dominant role in enhancing anaerobic denitrification. During this process, the nitrate removal rate was significantly increased by 40.60% at 11 h with little nitrite and N2O accumulation, which was attributed to the enhanced activities of the electron transport system and various denitrifying reductases. Based on proteomics and metabolomic analysis, N, Fe-CDs effectively regulated carbon/nitrogen/sulfur metabolism to induce more electron generation, less nitrite/N2O accumulation, and higher levels of nitrogen removal. This work reveals the mechanism by which N, Fe-CDs enhance anaerobic denitrification and broaden their potential application in nitrogen removal.
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Desnitrificação , Nitritos , Nitritos/metabolismo , Nitritos/farmacologia , Carbono , Anaerobiose , Proteômica , Nitrogênio/metabolismo , Nitrogênio/farmacologiaRESUMO
Anaerobic digestion is an environmentally friendly method for reclaiming waste activated sludge. However, it cannot be overlooked that the solid residue generated from this process can still pose environmental risks and impose economic pressure on society. To mitigate and recycle the solid residue, this study utilized it as a primary raw material for manufacturing ceramsite with potential applications in wastewater treatment. The optimal ratio of solid residue to fly ash was demonstrated to be 6:4 with an additional 15% of clay supplementing the raw ceramsite materials. Furthermore, the optimal sintering process was established as preheating at 300 °C for 25 min followed by sintering at 1085 °C for 10 min, as determined through an L16 (44) Orthogonal test. The prepared ceramsite demonstrated advantageous performance parameters that exceeded the standards outlined in the Chinese industry standard CJ/T 299-2008 for water treatment artificial ceramsite. When utilized in an ozonation system, the ceramsite exhibited remarkable catalytic activity for phenol degradation by promoting the decomposition of molecular O3 into hydroxyl radicals. Additionally, it displayed minimal leaching of heavy metals and lower application costs. These findings emphasize its attractiveness in water and wastewater treatment processes and present a practical strategy for reclaiming this solid residue.
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Misturas Complexas , Metais Pesados , Ozônio , Esgotos , Anaerobiose , Metais Pesados/análise , Cinza de Carvão , Resíduos SólidosRESUMO
This study investigated the removal performance of ofloxacin (OFL) by a novel electro-Fenton enhanced microfiltration membrane. The membranes used in this study consisted of metal-organic framework derived porous carbon, carbon nanotubes and Fe2+, which were able to produce hydroxyl radicals (â¢OH) in-situ via reducing O2 to hydrogen peroxide. Herein, membrane filtration with bias not only concentrated the pollutants to the level that could be efficiently treated by electro-Fenton but also confined/retained the toxic intermediates within the membrane to ensure a prolonged contact time with the oxidants. After validated by experiments, the applied bias of -1.0 V, pH of 3 and electrolyte concentration of 0.1 M were the relatively optimum conditions for OFL degradation. Under these conditions, the average OFL removal rate could be reach 75% with merely 5% membrane flux loss after 4 cycles operation by filtrating 1 mg/L OFL. Via decarboxylation reaction, piperazinyl ring opening, dealkylation and ipso substitution reaction, etc., OFL could be gradually and efficiently degraded to intermediate products and even to CO2 by â¢OH. Moreover, the oxidation reaction was preferred to following first-order reaction kinetics. This research verified a possibility for antibiotic removal by electro-enhanced microfiltration membrane.
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Nanotubos de Carbono , Poluentes Químicos da Água , Ofloxacino , Porosidade , Antibacterianos , Oxidantes , Peróxido de Hidrogênio , OxirreduçãoRESUMO
Aromatic azo dyes possess inherent resistance and are known to be carcinogenic, posing a significant threat to human and ecosystems. Enhancing the biodegradation of azo dyes usually requires the presence of co-metabolic substrates to optimize the process. In addressing the issue of excessive waste activated sludge (WAS) generation, this study explored the potential of utilizing alkaline-thermal hydrolysate of WAS as a co-metabolic substrate to boost the degradation of reactive black 5 (RB5) dyes. The acclimated microbial consortium, when supplemented with the WAS hydrolysate obtained at a hydrolysis temperature of 30 °C, achieved an impressive RB5 decolorization efficiency of 90.3% (pH = 7, 35 °C) with a corresponding COD removal efficiency of 45.0%. The addition of WAS hydrolysate as a co-substrate conferred the consortium with a remarkable tolerance to high dye concentration (1500 mg/L RB5) and salinity levels (4-5%), surpassing the performance of conventional co-metabolic sugars in RB5 degradation. 3D-EEM analysis revealed that protein-like substances rich in tyrosine and tryptophan, present in the WAS hydrolysate, played a crucial role in promoting RB5 biodegradation. Furthermore, the microbial consortium community exhibited an enrichment of dye-degrading species, including Acidovorax, Bordetella, Kerstersia, and Brevundimonas, which dominated the community. Notably, functional genes associated with dye degradation and intermediates were also enriched during the RB5 decolorization and biodegradation process. These findings present a practical strategy for the simultaneous treatment of dye-containing wastewater and recycling of WAS.
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The presence of the sulfonic acid group in sulfonated anthraquinones (SAs) resulted in the difficulty in the mineralization of anthraquinone ring. Little information is available on the removal pathway of the sulfonic acid group of SAs under aerobic/anaerobic conditions. Herein, sodium 1-aminoanthraquinone-2-sulfonate (ASA-2) was used as an important intermediate of SAs. A novel Pseudomonas nitroreducens WA capable of ASA-2 desulfonation was isolated from the Reactive Blue 19-degrading consortium WRB. Anaerobic desulfonation efficiency of 0.165 mM ASA-2 by strain WA reached 99% in 36 h at pH 7.5 and 35 â using glucose as an electron donor. Further analysis showed that ASA-2 as an electron acceptor could be anaerobically transformed into 1-aminoanthraquinone and sulfite via the cleavage of C-S bond. Strain WA could also desulfonate sodium 1-amino-4-bromoanthraquinone-2-sulfonate and sodium anthraquinone-2-sulfonate. Under denitrification conditions, the formed sulfite could be oxidized to sulfate by nitrite via a chemical reaction, which was beneficial for nitrite removal. This phenomenon was observed in consortium WRB-amended system. Moreover, the consortium WRB could reduce the formed sulfite to sulfide due to the presence of Desulfovibrio. These results provide a theoretical basis for the anaerobic biodesulfonation of SAs along with nitrate removal and support for the development of sulfite-based biotechnology.
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Nitratos , Ácidos Sulfônicos , Nitratos/metabolismo , Nitritos , Anaerobiose , Antraquinonas , Alcanossulfonatos , Biotransformação , Sulfitos , DesnitrificaçãoRESUMO
Ketoprofen (KTP) as a non-steroidal anti-inflammatory drug has been detected in coastal environment due to its wide usage. However, little information is available about the fate of KTP in marine environment. In the present study, the aerobic degradation of 20 mg L-1 KTP using the enriched marine consortia was investigated. Results showed that CA consortium cultured with casamino acids exhibited a higher KTP-degrading ability than those cultured with glucose, yeast extract and mixed vitamins. During CA consortium-mediated degradation of KTP, additional casamino acids resulted in the production of H2O2 and OH. Fe(III) could be also reduced to Fe(II) by CA consortium. This result indicated the occurrence of Fenton-like reaction. Further studies found that both biogenic Fenton-like reaction and enzyme-catalyzed reactions were involved in the initial hydroxylation reaction of KTP, then the subsequent mineralization of KTP was only performed via enzyme-catalyzed reactions. High-throughput sequencing analysis showed that Halomonas, Marinobacter, Owenweeksia and Oceanimonas were significantly enriched in CA consortium. As these genera contain amino acid oxidases, and the former two genera are capable of reducing Fe(III), it is assumed that these genera participated in biogenic Fenton-like reaction. The involvement of biogenic Fenton-like reaction provides a new insight into understanding the fate of KTP and other similar organic pollutants in marine environment containing amino acids and iron.
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Compostos Férricos , Cetoprofeno , Peróxido de Hidrogênio/química , Oxirredução , Ferro/químicaRESUMO
With growing demand for freshwater resources, membrane distillation (MD) attracts intensive attention owing to the possibility of reclaiming almost 100% freshwater with superhydrophobic membranes as the pivotal separation units. Current superhydrophobic membrane still suffers relatively complex preparation process and limited membrane flux. Herein, we developed a promising route to fabricate a high-flux superhydrophobic poly(vinylidene fluoride) (PVDF) membrane by a simple solute and solvent co-crystallization (SSCC) method, which endowed the membrane ultra-high porosity and flux. We also found that the pore size of superhydrophobic membrane can be adjusted by controlling the crystallization process of DMSO, which gave rise to membrane higher flexibility. The membrane exhibited the outperforming desalination performance even in multiple harsh environments including different temperature, salty concentration, and pH, with/without humic acid. The membrane also displayed distinguished anti-fouling performance and long-term stability, which is quite significant for practical application.
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In this study, three cold-tolerant phenol-degrading strains, Pseudomonas veronii Ju-A1 (Ju-A1), Leifsonia naganoensis Ju-A4 (Ju-A4), and Rhodococcus qingshengii Ju-A6 (Ju-A6), were isolated. All three strains can produce cis, cis-muconic acid by ortho-cleavage of catechol at 12 â. Response surface methodology (RSM) was used to optimize the proportional composition of low-temperature phenol-degrading microbiota. Degradation of phenol below 160 mg L-1 by low-temperature phenol-degrading microbiota followed first-order degradation kinetics. When the phenol concentration was greater than 200 mg L-1, the overall degradation trend was in accordance with the modified Gompertz model. The experiments showed that the microbial agent (three strains of low-temperature phenol-degrading bacteria were fermented separately and constructed in the optimal ratio) could completely degrade 200 mg L-1 phenol within 36 h. The above construction method is more advantageous in bio-enhanced treatment of actual wastewater. Through the construction of microbial agents to enhance the degradation effect of phenol, it provides a feasible scheme for the biodegradation of phenol wastewater at low temperature and shows good application potential.
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Fenol , Águas Residuárias , Fenol/metabolismo , Temperatura , Fenóis/metabolismo , Temperatura Baixa , Biodegradação AmbientalRESUMO
The phosphate adsorption behavior on MgO-modified diatomite has been routinely investigated. Batch experiments tend to show that the addition of NaOH during preparation largely promoted adsorption performance, but comparative studies of MgO-modified diatomite with and without NaOH (MODH and MOD) based on morphology, composition, functional groups, isoelectric points and adsorption behavior have not been reported. We demonstrated that NaOH can etch the structure of MODH and promote the migration of phosphate to active sites, which allowed MODH to have a faster adsorption rate, superior environmental adaptability, adsorption selectivity and regeneration performance. The phosphate adsorption ability was enhanced from 96.73 (MOD) to 197.4 mg P/g (MODH) under optimum conditions. Furthermore, the partially hydrolyzed Si-OH group reacted with Mg-OH via a hydrolytic condensation reaction to form a new Si-O-Mg bond. Intraparticle diffusion, electrostatic attraction and surface complexation may be the main modes of phosphate adsorption by MOD, while the MODH surface mainly relied on the synergy of chemical precipitation and electrostatic attraction due to the abundant MgO adsorptive sites. Indeed, the present study provides a new understanding of the microscopic analysis of sample differences.
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Additional various carbon and free riboflavin could improve anaerobic digestion of waste activated sludge (WAS). However, these substances were not reused. In this study, a reusable riboflavin and carbon black (RCB) co-modified filler was developed and combined with alkaline pretreatment for enhancing the production of volatile fatty acids (VFAs) and methane during anaerobic digestion of WAS. The results showed that RCB-modified fillers exhibited a promoting effect on the reduction of alkali-pretreated WAS. The amounts of the accumulated VFAs mainly containing acetate and the produced methane rose with the increased concentration of immobilized riboflavin (0-0.75 g/L) in the presence of 4 g/L carbon black. When the alkaline pretreatment time of WAS increased from 3 d to 8 d, the amount of methane production increased from 22.8% to 63.9% in the presence of 0.75 g/L riboflavin and 4 g/L carbon black compared with that without RCB-modified fillers. Moreover, 0.75 g/L riboflavin and 4 g/L carbon black had a synergetic effect on promoting methane production via broadening extracellular electron transfer pathways. During this process, microbial dehydrogenase activity, electron transport system activity and coenzyme F420 were enhanced. Microbial community analysis showed that RCB-modified filler addition promoted the enrichment of Syntrophomonas and Pseudomonas involved in direct interspecies electron transfer (DIET). These results indicated that DIET establishment was accelerated. Meanwhile, the populations of acetic acid-producing bacteria including Rikenellaceae_RC9_gut_group and Proteiniphilum, aceticlastic and acid-tolerant methanogenic archaea including Methanosarcina and Methanosaeta, RumEn_M2 were increased. These results indicate that RCB-modified fillers coupled with alkaline pretreatment is an effective method to promote the production of methane during anaerobic digestion of WAS.
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Esgotos , Fuligem , Esgotos/microbiologia , Anaerobiose , Reatores Biológicos , Ácidos Graxos Voláteis , Metano , Bacteroidetes , Eliminação de Resíduos Líquidos/métodosRESUMO
The anaerobic ammonium oxidation (anammox), a microbial process that is considered as a low-cost and high efficient wastewater treatment, has received extensive attention with an attractive application prospect. The anammox process reduces nitrite (NO2-) to nitrogen gas (N2) with ammonium (NH4+) as the electron donor. However, some nitrate (NO3-) equivalent to 11% of total nitrogen (TN) is generated in this process, which limits the development of anammox. To overcome this problem, many efforts have been made in this regard, mainly combining with other biological treatment methods (denitrification, denitrifying anaerobic methane oxidation, etc.), introducing the substance into anammox process, etc. Herein, we summarized a detailed review of previous researches on the removal of NO3- in the anammox-dependent processes. It is hoped that this review could serve as valuable guidance in future research and practical applications of anammox.
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Oxidação Anaeróbia da Amônia , Nitratos , Nitritos , Metano , NitrogênioRESUMO
As two important components of dissolved organic matter (DOM), dissolved black carbon (DBC) and humic acid (HA) possess different chemical and structural properties, which might influence their activities like metal complexation and mediating electron transfer. In this study, a series of coprecipitates of iron oxides (FeOx) and DOM (HA or DBC) having different C/Fe molar ratios (0.2-3.0) was prepared under ambient conditions, which exhibited excellent catalytic efficiencies upon Fenton-like degradation of norfloxacin (NOR). Pseudo-first-order rate constant of NOR oxidation catalyzed by DBC-FeOx (C/Fe=3.0, 1.13 h-1) was 30.5, 4.3-14.2, and 1.3-15.7 folds higher than those mediated by FeOx alone, HA-FeOx and DBC-FeOx coprecipitates having C/Fe molar ratios of 0.2 and 1.6, respectively. Due to the higher concentrations of surface-bound Fe(III)/Fe(II) in the DBC-FeOx mediated systems, improved Fe(III)/Fe(II) cycling rates, â¢OH accumulation and NOR degradation were observed as compared with those of counterpart systems mediated by HA-FeOx. Besides functioning in Fe-C complexation to accelerate FeOOH cleavage, carbonyl/carboxyl groups of the coprecipitates also serve as electron shuttles, both of which improved Fe(III)/Fe(II) cycling and â¢OH production. Our findings emphasized the influence of DOM source and compositions on Fe(III)/Fe(II) cycling and provided a facile approach of preparing Fe-C catalyst for contaminants elimination.
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Compostos Férricos , Norfloxacino , Matéria Orgânica Dissolvida , Fuligem , Compostos Ferrosos , Óxidos , FerroRESUMO
We propose a novel sulfide-driven process to recover N2O during the traditional denitrification process. The optimum initial sulfide concentration was 120 mg/L, and the N2O percentage in the gaseous products (N2O+N2) was up to 82.9%. Moreover, sulfide involved in denitrification processes could substitute for organic carbon as an electron donor, e.g., 1 g sulfide was equivalent to 0.5-2 g COD when sulfide was oxidized to sulfur and sulfate. The accumulation of N2O was mainly due to the inhibiting effect of sulfide on nitrous oxide reductase (N2OR), which was induced by the supply insufficiency of electrons from cytochrome c (cyt c) to N2OR. When the initial sulfide concentration was 120 mg/L, the N2OR activity was only 36.8% of its original level. According to the results of cyclic voltammetry, circular dichroism spectra and fluorescence spectra, significant changes in the conformations and protein structures of cyt c were caused by sulfide, and cyt c completely lost its electron transport capacity. This study provides a new concept for N2O recovery driven by sulfide in the denitrification process. In addition, the findings regarding the mechanism of the inhibition of N2OR activity have important implications both for reducing emissions of N2O and recovering N2O in the sulfide-driven denitrification process.
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Desnitrificação , Óxido Nitroso , Óxido Nitroso/química , Sulfetos , Enxofre , OxirreduçãoRESUMO
After the anaerobic digestion of excess sludge, dissolved organic matter is absorbed and used, but the treatment of refractory sludge is a headache. The oxidative acid (performic acid and hydrochloric acid) hydrolysis process can effectively prepare amino acids from refractory sludge. During the preparation process, insoluble proteins in sludge were turned into soluble proteins and peptides. All of them eventually hydrolyse into amino acids. The optimum conditions in the single-factor experiment were as follows: a temperature of 110°C, a reaction time of 24â h, and a hydrochloric acid (HCl) concentration of 6â M. The results showed that the maximum total yield of amino acids from refractory sludge was 94.76%. In the orthogonal experiment, the maximum total yield of amino acids was 97.20% under the optimum conditions of a temperature of 113.45°C, a reaction time of 26.79 h, and 5.92 M HCl. The recovery rate of purity amino acids was 17.16 g per 100 g of dry sludge. The recovery rate of the hydrochloric acid was approximately 70%. There were 17 kinds of amino acids in the hydrolysate, which could be used as deodorants, food additives, preservatives, and corrosion inhibitors. This new technology is expected to be very effective in the treatment of refractory sludge.
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Dissimilatory nitrate reduction to ammonium (DNRA) can be used for nitrogen recovery. However, due to the low conversion efficiency of the DNRA process of microorganisms, the process cannot be industrially applied. Ascorbic acid (ASA) can improve DNRA efficiency of Escherichia coli sp. SZQ1 (E. coli). Experimental studies suggest that 10 g L-1 ASA promoted DNRA process of E. coli at high concentrations of nitrite (10-20 mM). In the 5 g L-1 ASA system, 9.2 mM nitrite was reduced to 8.21 mM ammonium by E. coli in 120 h. Mechanistic studies reveal that ASA reduced the oxidation-reduction potential (ORP) of the system and scavenged reactive oxygen species (ROS) in the cell of E. coli. Meanwhile, ASA was utilized by E. coli as the sole carbon source and provided electrons to DNRA process through ASA metabolic pathways. This study proposes a new strategy for increasing the efficiency of DNRA.