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Environmental pollution stands as one of the significant global challenges we face today. Polycyclic aromatic hydrocarbons (PAHs), a class of stubborn organic pollutants, have long been a focal point of bioremediation research. This study aims to explore the impact and mechanisms of graphene oxide (GO) on the phytoremediation effectiveness of PAHs. The results underscore the significant efficacy of GO in accelerating the degradation of PAHs. Additionally, the introduction of GO altered the diversity and community structure of endophytic bacteria within the roots, particularly those genera with potential for PAH degradation. Through LEfSe analysis and correlation studies, we identified specific symbiotic bacteria, such as Mycobacterium, Microbacterium, Flavobacterium, Sphingomonas, Devosia, Bacillus, and Streptomyces, which coexist and interact under the influence of GO, synergistically degrading PAHs. These bacteria may serve as key biological markers in the PAH degradation process. These findings provide new theoretical and practical foundations for the application of nanomaterials in plant-based remediation of polluted soils and showcase the immense potential of plant-microbe interactions in environmental restoration.
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Bactérias , Biodegradação Ambiental , Grafite , Hidrocarbonetos Policíclicos Aromáticos , Microbiologia do Solo , Poluentes do Solo , Grafite/química , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Poluentes do Solo/metabolismo , Bactérias/efeitos dos fármacos , Bactérias/metabolismo , Endófitos/metabolismo , Raízes de Plantas/microbiologia , Sphingomonas/metabolismo , Plantas/microbiologia , Plantas/metabolismo , Mycobacterium/efeitos dos fármacos , Mycobacterium/metabolismo , Flavobacterium/efeitos dos fármacos , Flavobacterium/metabolismo , Streptomyces/metabolismo , Microbacterium/metabolismoRESUMO
We report three heterozygous PROS1 mutations that caused type I protein S deficiency in three unrelated Chinese families. We measured protein S activity and antigen levels for all participants, screened them for mutations in the PROS1 gene. And we employed the calibrated automated thrombin generation (CAT) method to investigate thrombin generation. Numerous bioinformatics tools were utilized to analyze the conservation, pathogenicity of mutation, and spatial structure of the protein S. Phenotyping analysis indicated that all three probands exhibited simultaneous reduced levels of PS:A, TPS:Ag, and FPS:Ag. Genetic testing revealed that proband A harbored a heterozygous c.458_458delA (p.Lys153Serfs*6) mutation in exon 5, proband B carried a heterozygous c.1687C>T (p.Gln563stop) mutation in exon 14, and proband C exhibited a heterozygous c.200A>C (p.Glu67Ala) mutation in exon 2. Bioinformatic analysis predicted that the p.Lys153Serfs*6 frameshift mutation and the p.Gln563stop nonsense mutation in the protein S were classified as "disease-causing." The identification of the novel mutation p.Lys153Serfs*6 in PROS1 enriches the Human Genome Database. Our research suggests that these three mutations (p.Lys153Serfs*6, p.Gln563stop, and p.Glu67Ala) are possibly responsible for the decreased level of protein S in the three families. Furthermore, the evidence also supports the notion that individuals who are asymptomatic but have a family history of PSD can benefit from genetic analysis of the PROS1 gene.
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Proteínas Sanguíneas , Deficiência de Proteína S , Humanos , Proteínas Sanguíneas/genética , Deficiência de Proteína S/diagnóstico , Deficiência de Proteína S/genética , Trombina , Mutação , China , Linhagem , Proteína S/genéticaRESUMO
Pulsed electric field (PEF) technology has attracted considerable attention because it can efficiently treat pollutants that are difficult to degrade. In this study, a PEF system using iron as the electrode was constructed to investigate the effect of PEF-Fe on the growth and metabolism of aerobic denitrifying bacteria and the effectiveness of wastewater nitrogen removal. The chemical oxygen demand, NO3--N and nitrate removal rates were 98.93%, 97.60% and 24.40 mg·L-1·h-1, respectively, under optimal conditions. As confirmed in this study, PEF-Fe could improve the key enzyme activities of W207-14. Scanning electron microscopy revealed that the surface of PEF-Fe-treated W207-14 was intact and smooth without any irreversible deformation. Flow cytometry combined with fluorescence staining analysis also confirmed reversible electroporation on the cell membrane surface of PEF-Fe-treated W207-14. Differentially expressed gene enrichment analysis showed that PEF-Fe activated the transmembrane transport function of ATP-binding cassette transporte (ABC) transport proteins and enhanced the cell membrane permeability of aerobic denitrifying bacteria. The significant differential expression of iron-sulphur cluster proteins facilitated the regulation of electron transport and maintenance of the dynamic balance of iron ions within the PEF-Fe system.
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Ferro , Nitrogênio , Nitrogênio/metabolismo , Desnitrificação , Bactérias/metabolismo , EletrodosRESUMO
Simulated Annealing (SA) algorithm is not effective with large optimization problems for its slow convergence. Hence, several parallel Simulated Annealing (pSA) methods have been proposed, where the increase of searching threads can boost the speed of convergence. Although satisfactory solutions can be obtained by these methods, there is no rigorous mathematical analyses on their effectiveness. Thus, this article introduces a probabilistic model, on which a theorem about the effectiveness of multiple initial states parallel SA (MISPSA) has been proven. The theorem also demonstrates that the increasing parallelism in pSA algorithm with the reducing of search depth in each thread could obtain almost the same probability of finding the global optimal solution. We validated our theorem on AutoDock Vina, a widely used molecular docking tool with high accuracy and docking speed. AutoDock Vina uses a pSA strategy to find optimal molecular conformations. Under the premise that the total searching workload (i.e., thread number * iteration depth of each thread) remains unchanged, the docking accuracy from an aggressively parallelized SA searching method is almost the same or even better than those from the default exhaustiveness (parallelism degree) configuration of AutoDock Vina. Taking complex '1hnn' as an example,with the increase (125x) in the number of initial states (from 8 to 1000) and the decrease in the search depth for each thread (from 15540 to 124, or 1/125 of the original search depth), the mean energy is -7.80 and -7.94, while the mean RMSD is 3.4 and 3.14, respectively. The result also implies that a considerable speedup (in this case 125x in theory) can be obtained by a highly parallelized SA algorithm implementation.
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Algoritmos , Proteínas , Simulação de Acoplamento Molecular , Ligantes , SoftwareRESUMO
Freshwater lake eutrophication is a global concern causing adverse effects on aquatic ecosystems. The degradation of lake aquatic-terrestrial ecotones, which are the transitional zones between terrestrial and water ecosystems, contributes to eutrophication. These ecotones play vital roles in nutrient cycling, runoff control, biodiversity conservation, and habitat provision. In the past three decades, the research on lake aquatic-terrestrial ecotones has focused on techniques for managing contaminants and runoff purification. This paper reviews the recent studies on the restoration ability of eutrophic water bodies in lake aquatic-terrestrial ecotones in recent years regarding three aspects: the establishment, restoration mechanism, and improvement of restoration function. In addition, ecological factors such as lakeshore height, water level, surface runoff, shallow groundwater level, and rainfall intensity have impacts on the restoration capacity of lake aquatic-terrestrial ecotones.
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To better understand how fish communities respond to environmental changes under extreme climate events, we examine changes in fish communities in Beibu Gulf during strong El Niño and La Niña events. Strong La Niña and El Niño events affect the composition, abundance, and distribution of fish communities in Beibu Gulf. Fish community distribution and composition change before and after La Niña and El Niño events, and dominant species within them change with stable fishing intensity. The abundance and distribution of small pelagic fish such as Japanese jack mackerel (Trachurus japonicus) and Japanese scad (Decapterus maruadsi) are the most affected. Using a generalized additive model (GAM), we explore relationships between the abundance of T. japonicus and D. maruadsi and a suite of environmental variables. The GAM results revealed that sea surface salinity and sea surface temperature best explain changes in catch per unit effort of these two species during a La Niña event; depth, sea surface temperature, and mixed layer depth during an El Niño event. The results obtained in this study will offer support for implementing more-accurate, scientific fisheries management measures.
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The smart photochromic materials based on polylactic acid (PLA) were prepared by melt-blending and hot-pressing, in which photochromic microcapsules (PM) were used as a functional additive, and poly(vinyl acetate) (PVAc) was introduced into the photochromic PLA blends for the first time to improve their properties. The crystallization and melting behavior, morphology, and photochromic performance of PLA/PVAc/PM blends were characterized by differential scanning calorimetry (DSC), scanning electron microscopy (SEM), and spectrophotometer, respectively. The results showed that PVAc significantly improved the photochromic properties of PLA/PM blends. Under 30 s UV irradiation, the blends reached a value of ΔE that could be recognized in 3 s by human eyes. This discriminative ΔE value could be maintained for at least 3 min after removal from UV irradiation. Meanwhile, the blend had outstanding photochromic durability and recyclability. Compared to ΔE for 0.5 h of continuous light irradiation, ΔE for 8 h of continuous light irradiation decreased by only about 1, to 14.1. In 25 cycles of 3 s UV irradiation, the values of ΔE for the first and 25th irradiation were 11.4 and 11.6, respectively. The blend showed different photochromic responses to different light intensities. The ΔE values of 8.6, 14.6, 14.6, and 18.4 for irradiation at 600, 800, 1000, and 1200 W/m2 of solar intensity, respectively.
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Ácido Láctico , Polímeros , Humanos , Polímeros/química , Ácido Láctico/química , Poliésteres/química , Varredura Diferencial de CalorimetriaRESUMO
Due to the annual increase in wastewater treatment in most Chinese cities, a major environmental issue has arisen: safe treatment, disposal, and recycling of municipal sludge. Municipal sludge has a high content of carbon and essential nutrients for plant growth; hence, it has gained interest among researchers as a soil fertilizer. This study discusses the potential usage of municipal sludge as soil fertilizer (indicators include nitrogen (N), phosphorus (P), and trace elements) along with its shortcomings and drawbacks (potentially toxic elements (PTEs), organic matter (OM), pathogens, etc.) as well as reviews the latest reports on the role of municipal sludge in land use. The use of municipal sludge as a soil fertilizer is a sustainable management practice and a single application of sludge does not harm the environment. However, repeated use of sludge may result in the accumulation of harmful chemicals and pathogens that can enter the food chain and endanger human health. Therefore, long-term field studies are needed to develop ways to eliminate these adverse effects and make municipal sludge available for agricultural use.
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Hydrometallurgy technology can directly deal with low grade and complex materials, improve the comprehensive utilization rate of resources, and effectively adapt to the demand of low carbon and cleaner production. A series of cascade continuous stirred tank reactors are usually applied in the gold leaching industrial process. The equations of leaching process mechanism model are mainly composed of gold conservation, cyanide ion conservation, and kinetic reaction rate equations. The derivation of the theoretical model involves many unknown parameters and some ideal assumptions, which leads to difficulty and imprecision in establishing the accurate mechanism model of the leaching process. Imprecise mechanism models limit the application of model-based control algorithms in the leaching process. Due to the constraints and limitations of the input variables in the cascade leaching process, a novel model-free adaptive control algorithm based on compact form dynamic linearization with integration (ICFDL-MFAC) control factor is first constructed. The constraints between input variables is realized by setting the initial value of the input to the pseudo-gradient and the weight of the integral coefficient. The proposed pure data-driven ICFDL-MFAC algorithm has anti-integral saturation ability and can achieve faster control rate and higher control precision. This control strategy can effectively improve the utilization efficiency of sodium cyanide and reduce environmental pollution. The consistent stability of the proposed control algorithm is also analyzed and proved. Compared with the existing model-free control algorithms, the merit and practicability of the control algorithm are verified by the practical leaching industrial process test. The proposed model-free control strategy has advantages of strong adaptive ability, robustness, and practicability. The MFAC algorithm can also be easily applied to control the multi-input multi-output of other industrial processes.
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Polylactic acid/butenediol vinyl alcohol copolymer (PLA/BVOH) blends with different weight ratios were prepared by melt mixing. PLA and BVOH in PLA/BVOH blends were immiscible while the weak interaction between PLA and BVOH existed. The introduction of BVOH facilitated the crystallization of PLA. Moreover, the crystallization of PLA hindered the crystallization of BVOH. Due to introduction of BVOH, PLA/BVOH blends exhibited shear thinning characteristic except that PLA/BVOH blends with 5-10 % BVOH showed similar rheological property to neat PLA. With the increase of BVOH content, the contact angle of PLA/BVOH blends decreased from 79.75° to 67.33° at 120 s. The hydrophilicity of PLA/BVOH blends was improved. In addition, PLA/BVOH fibers with 5-40 % BVOH and PLA/BVOH/rutin fibers with 3 % rutin were manufactured by melt spinning. The effect of BVOH on the mechanical property of PLA/BVOH fibers was small. However, BVOH improved significantly the rutin release rate and antioxidant properties of PLA/BVOH/rutin fibers.
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Poliésteres , Polímeros , Polímeros/química , Poliésteres/química , Cristalização , Etanol , Compostos de VinilaRESUMO
A comparative study was conducted to determine whether hesperetin and pectolinarigenin could lower total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL), and high-density lipoprotein cholesterol (HDL) in a high-fat diet (HFD)-induced high lipid model in Golden Syrian hamsters. 48 Golden Syrian hamsters (8 weeks old) were fed with a HFD for 15 days. HFD induced significant increases in plasma TC, TG, LDL, and HDL. Then, these high lipid hamsters were divided into four groups and were administered with 0.5% sodium carboxymethyl cellulose (CMC-Na), hesperetin (100 mg/kg/day), pectolinarigenin (100 mg/kg/day) or atorvastatin (1.0 mg/kg/day), for 7 weeks. It was found that pectolinarigenin treatment resulted in significant reductions in body weight, adiposity index, serum levels of TC, TG and hepatic TC, TG and free fatty acid compared to those in control hamsters with hyperlipidemia (P<0.05). However, hesperetin treatment only caused reductions in plasma TC and hepatic TG levels. Besides, the hamsters treated with pectolinarigenin showed a relatively normal hepatic architecture compared to the hepatic steatosis shown in the control group. Moreover, the expressions of fatty-acid synthase (Fasn) and solute carrier family 27 member 1 (Slc27a1) involved in lipid biosynthesis, were suppressed in the pectolinarigenin-treated groups, and the expression of carnitine palmitoyltransferase 1A (Cpt1a) involved in fatty acid oxidation was increased in the pectolinarigenin-treated group. Taken together, these results suggest pectolinarigenin exerts stronger protective effects against hyperlipidemia and hepatic steatosis than hesperetin, which may involve the inhibition of lipid uptake and biosynthesis.
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Fígado Gorduroso , Hiperlipidemias , Cricetinae , Animais , Mesocricetus , Hiperlipidemias/etiologia , Dieta Hiperlipídica , Fígado/metabolismo , Triglicerídeos/metabolismo , Triglicerídeos/farmacologia , LDL-Colesterol/metabolismo , LDL-Colesterol/farmacologiaRESUMO
There is a high rate of recurrent hypertriglyceridemic acute pancreatitis (HTG-RAP) and risk of developing into chronic pancreatitis among recurrent hypertriglyceridemic acute pancreatitis. The key to avoiding recurrence is home-based self-management. However, self-management has proven to be difficult. Exploring experiences and perceptions of home-based self-management among patients with HTG-RAP could inform intervention development and policy making in primary care. To explore experiences and perceptions of home-based self-management among patients with HTG-RAP. This is primarily a qualitative study involving patients from eastern China. The study was designed using semi-structured interviews combined with open interviews among individuals and focus groups. Interviews with patients (n = 25) and relatives (n = 2) were conducted from October to December, 2021. Data were analyzed using the thematic analysis approach. Five themes were identified: (1) pity, (2) sense of uncertainty, (3) contradiction, (4) the way to cope, and (5) benefits. The themes constituted a continuous process where a final coping strategy was confirmed. Patients expressed sorrow, struggle, pity, adaptation, and benefits. The disease still bothered them without attack, both mentally and physically. These key points deserve considerable attention to improve the quality of life of patients and lifestyle modification. Patients with pancreatitis were more likely to manage the disease but under a tough process, and during the struggle, they experienced a continuous and contradictory period. Ultimately, the final condition was reached.
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Pancreatite Crônica , Qualidade de Vida , Humanos , Doença Aguda , Pesquisa Qualitativa , Pancreatite Crônica/terapiaRESUMO
OBJECTIVE: To explore the molecular mechanisms of a Chinese pedigree with hereditary factor â ª (Fâ ª) deficiency. METHODS: All of the 15 exons, flanking sequences of the Fâ ª gene and the corresponding mutation sites of family members were analyzed by the Sanger sequencing, followed by the extraction of the peripheral blood genomic DNA. And all the results were verified by the reverse sequencing. The conservation of the mutated sites was analyzed by the ClustalX-2.1-win. Three online bioinformatics software tools, including Mutation Taster, PolyPhen2 and the PROVEAN, were used to assess the possible impact of the mutations. Swiss-pdbviewer software was used to analyze the effects of mutant amino acids on protein structure. RESULTS: Genetic analysis revealed that the proband had compound heterozygous mutations including a nonsense mutation of c.1107C>A (Tyr369stop) in exon 10 and missense mutation of c.1562A>G (Tyr521Cys) in exon 13. The same c.1107C>A (Tyr369stop) was present in her father, the same c.1562A>G (Tyr521Cys) was present in both her mother and daughter. Conservation analysis indicated that Tyr521 was a highly conserved site during evolution. The prediction of pathogenicity showed that both c.1107C>A and c.1562A>G were pathogenic mutations. Protein structure prediction showed that in the wild type Fâ ª protein structure, Tyr521 formed a hydrogen bond with the Lys572 and Ile388, respectively. When Tyr521 was replaced by Cys521, the original benzene ring structure disappeared, and side chains of Lys572 added a hydrogen bond with the Cys521, which may change protein catalytic domain structure. When Tyr369 was mutated to a stop codon, resulting in the truncated protein. CONCLUSION: The compound heterozygous mutations including the c.1107C>A heterozygous missense variant in exon 10 and the c.1562A>G heterozygous nonsense mutation in exon 13 may be responsible for the hereditary factor â ª deficiency in this Chinese pedigree.
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Deficiência do Fator XI , Fator XI , Códon sem Sentido , Fator XI/genética , Deficiência do Fator XI/genética , Feminino , Heterozigoto , Humanos , Mutação , LinhagemRESUMO
With China's industrialization and a rapidly developing coal industry, tailings have become one of the most widely distributed solid wastes, responsible for degrading available land and damaging the surrounding ecological environment. This study investigated the effect of adding mineral-microbial complexes to tailing substrates for the improvement of plant growth and substrate microbial community. The results revealed that compared with other treatments, the growth of Rorippa was considerably better after the addition of mineral-microbial complexes to the substrate, indicating that the mineral-microbial complexes promoted plant growth. After the addition of mineral-microbial complexes, the fertility indicators of the substrate showed a substantial improvement, in addition to the pH and organic matter (OM). The addition of fertilizers to the substrate plays a key role in plant growth, whereas the addition of microbial supplements to the substrate alone has little effect on plant growth. The results of high-throughput sequencing showed that the main microbial communities present in the substrate were Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, and Nitrospirae. The results of the microbial community α-diversity analysis showed that the addition of the mineral-microbial complexes improved the abundance and diversity of the substrate microbial community. Results of the microbial community ß-diversity analysis indicated that the experimental group showed a higher correlation with the microbial community relative to the background group. Network analysis revealed similar correlations between microbial communities and environmental factors, and total phosphorous (TP)-pH-available potassium (AK)-available nitrogen (AN) and TP-electronic conductivity (EC)-AK-AN were the main drivers of microbial communities in the background and experimental groups, respectively. The findings of this study provide a theoretical basis for the resource utilization of tailings and vegetation restoration using tailings.
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Microbiota , Rorippa , Bactérias/genética , Minerais , Nitrogênio/análise , Nutrientes , Solo/química , Microbiologia do SoloRESUMO
Objective: The purpose of this study was to compare the levels of 8-oxoG and 8-oxodG in urine of patients with cervical carcinoma and healthy women to evaluate their influences on cervical carcinoma. Methods: In this study, urine samples were collected from 70 patients with cervical carcinoma, 24 patients with one-year follow-up, and 100 healthy women. The contents of 8-oxodG and 8-oxoG in urine were assayed by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Results: The levels of 8-oxoG and 8-oxodG were higher in patients with cervical carcinoma (P < 0.000), while AUC of 8-oxoG and 8-oxodG was higher than 0.7. Specifically, the high-risk HPV (HR-HPV) positive group had higher 8-oxoG levels (P < 0.000), but there was no difference in 8-oxodG levels. Yet, 8-oxoG level was associated with lymphatic metastasis, lymph-vascular space infiltration (LVSI) and stromal infiltration, while 8-oxodG level was affected by the differentiation degree and stromal infiltration. According to statistics, the distinct cut-off index of lymphatic metastasis was 7.282 nmol/mmol creatinine. After operation, the concentrations of 8-oxoG and 8-oxodG dropped significantly (8-oxoG P < 0.000, 8-oxodG P = 0.004). Except for chemotherapy group, the urinary 8-oxoG dose of all treatment groups and 8-oxodG dose of chemo-radiotherapy group declined obviously. Conclusions: 8-oxoG may be a potential biomarker for cervical carcinoma.
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Functional language has been used in many multinational corporations (MNCs) as a way to overcome the problems caused by the coexistence of multiple languages in the workplace. The existing literature has explored the importance, adoption, and effectiveness of functional language. Yet, how functional language shapes host country employees' moral cognition and behavior is insufficiently researched. Guided by the Social Identity Theory, this manuscript shows that host country employees' functional language proficiency (i.e., English) enhances their unethical pro-organizational behavior through their linguistic group identification and moral disengagement. We tested our predictions using the data collected from 309 full-time host country employees through an online survey, and the results generally supported our hypotheses. The findings make contributions to both international management and language literature and organizational moral behavior literature.
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BACKGROUND: Recently, several studies have reported an association between lipid profiles and sudden sensorineural hearing loss (SSNHL), yet there is considerable variability between the individual studies in defining the precise association between serum lipids levels and SSNHL. This study sought to identify a possible relationship between dyslipidemia and the prevalence and prognosis of SSNHL. METHODS: A case-control study was carried out at two independent medical centers, including 2,288 SSNHL patients and 2,288 healthy controls. Clinical characteristics and serum lipid parameters were assessed, including total cholesterol (CHOL), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (Trig), apolipoprotein AI (ApoAI), apolipoprotein B (ApoB), and lipoprotein a (Lpa). Multivariate logistic regression analysis was performed to assess the relationship between lipid profiles and SSNHL in the 4,576 subjects. RESULTS: Significant differences were identified in several conventional serum lipid markers including CHOL, Trig, HDL, LDL, ApoAI, ApoB, and Lpa, between SSNHL patients and healthy controls. Serum ApoAI levels were significantly lower in patients with bilateral SSNHL compared to unilateral SSNHL. Binary logistic regression analysis revealed that higher levels of ApoB, LDL, Trig, and lower levels of ApoAI and HDL were all associated with an increased risk of SSNHL. After clinical characterization, multivariate analysis showed that only low levels of ApoB predicted likelihood of a recovery of more than 30 dB among patients with SSNHL. CONCLUSIONS: Serum lipids are associated with the incidence and prognosis of SSNHL. Identification of dyslipidemia may improve early evaluation and management of SSNHL risks.
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Apolipoproteína B-100/sangue , Dislipidemias/complicações , Perda Auditiva Neurossensorial/etiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Apolipoproteína A-I/sangue , Estudos de Casos e Controles , Criança , Perda Auditiva Neurossensorial/sangue , Perda Auditiva Neurossensorial/epidemiologia , Humanos , Incidência , Lipídeos/sangue , Lipoproteínas LDL/sangue , Masculino , Pessoa de Meia-Idade , Prognóstico , Fatores de Risco , Triglicerídeos/sangue , Adulto JovemRESUMO
In this study, three dominant bacteria Cellulomonas flavigena (â ), Cellulomonas flavigena (â ¡), Sphingomonas paucimobilis (â ¢) from Fire Phoenix rhizosphere soil were used to develop a multi-microbial agent system. For oil-contaminated soil in the Dagang oilfield, the immobilized test bacteria were inoculated into the Fire Phoenix rhizosphere soil to examine the effects of bacterial agents on polycyclic aromatic hydrocarbons (PAHs)-contaminated soil. The results showed that PAHs degradation was promoted under the â â ¢ (with an effective number of viable bacteria of 109 cfu·mL-1) and â â ¡â ¢ (with an effective number of viable bacteria of 107 cfu·mL-1) treatments. The PAHs degradation rates were 32.2% and 41.4%, respectively, being significantly higher than that in the control treatments. The â â ¡â ¢ treatment significantly promoted the belowground biomass of Fire Phoenix, which was 31.2% higher than that of the control treatment. Our results suggested that the multi-microbial agent constructed by the three dominant bacteria â â ¡â ¢ could be used as a strengthening method for the remediation of PAHs-contaminated soil by Fire Phoenix, which provided a novel method for microbial enhanced phytoremediation technology.
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Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Bactérias , Cellulomonas , Hidrocarbonetos Policíclicos Aromáticos/análise , Solo , Microbiologia do Solo , Poluentes do Solo/análise , SphingomonasRESUMO
3D-printed scaffolds have been developed as potential therapeutic strategies in bone tissue engineering. Mg/PCL biomaterials have been attracted much attention owing to biocompatibility, biodegradability as well as tunable mechanical properties. In this work, we developed 3D-printed customized Mg/PCL composite scaffolds with enhanced osteogenesis and biomineralization. Mg microparticles embedded in PCL-based scaffolds took a positive role in the improvement of biocompatibility, biomineralization, and biodegradable abilities. When incorporated with 3 wt% Mg, PCL-based scaffolds exhibited the optimal bone repairing ability in vitro and in vivo. The in vitro experiments indicated that 3 Mg/PCL scaffolds had improved mechanical properties, good biocompatibility, enhanced osteogenic and angiogenic activities. Besides, the in vivo studies demonstrated that Mg/PCL scaffolds promoted tissue ingrowth and new bone formation. In sum, these findings indicated that 3D-printed cell-free Mg/PCL scaffolds are promising strategies for bone healing application.