RESUMO
PURPOSE: Various time-lapse studies have postulated embryo selection criteria based on early morphokinetic markers. However, late paternal effects are mostly not visible before embryonic genome activation. The primary objective of this retrospective study was to investigate whether those early morphokinetic algorithms investigated by time-lapse imaging are reliable enough to allow for the accurate selection of those embryos that develop into blastocysts, while of course taking into account the correlation with the type of injected spermatozoa. METHODS: During a period of 18 months, a total of 461 MII oocytes from 43 couples with severe male factor infertility and previous "external" IVF failures after cleavage-stage embryo transfer (ET) were fertilized by intracytoplasmic morphologically selected sperm injection (IMSI). Thereof, 373 embryos were monitored in a time-lapse incubator until ET on day 5. Blastocyst outcome in combination with three previously postulated MKc (cc2: t3-t2, 5-12 h; t3, 35-40 h; t5, 48-56 h) and the morphology of the selected sperm were analyzed. RESULTS: A significant increase in the rate of blastocysts (54.0 vs. 36.3 %; P < 0.01) and top blastocysts (25.3 vs. 10.8 %; P < 0.001) was observed in the group of those meeting all three morphokinetic criteria (MKc3). However, MKc3 were only met in 23.3 % of all embryos. Moreover, TBR was influenced by the type of injected spermatozoa. In both groups, TBR decreased dramatically (MKc3, 35.0 vs. 17.0 %; MKc < 3, 14.2 vs. 8.4 %) when class II/III sperm instead of class I were injected. CONCLUSION: Early morphokinetic parameters might give some predictive information but fail to serve as a feasible selective tool for the prediction of blastocyst development given the influence of the type of spermatozoa injected.
Assuntos
Blastocisto/fisiologia , Espermatozoides/fisiologia , Imagem com Lapso de Tempo/métodos , Adulto , Algoritmos , Blastocisto/citologia , Feminino , Humanos , Processamento de Imagem Assistida por Computador , Infertilidade Masculina/etiologia , Masculino , Pessoa de Meia-Idade , Oócitos , Idade Paterna , Estudos Retrospectivos , Injeções de Esperma Intracitoplásmicas/métodosRESUMO
Time-lapse imaging is increasingly applied as an adjunct to reproductive medicine. The gained information of the morphological and morphokinetic variables before the onset of transcription are supposed to be good predictors for the selection of the best embryo for transfer and are often seen in line with clinical outcomes. This retrospective case series investigated the outcome of transferred blastocysts that did not fulfil the proposed embryo scores at early cleavage or at later stages of development. The observations were made by time-lapse imaging. This study reports the birth of 16 healthy children after day-5 blastocyst transfer, of which at least one of the transferred embryos originated from deviant morphology and/or kinetic cleavage patterns. This case series suggests that some blastocysts derived from embryos with poor conventional morphological score and/or suboptimal morphokinetics can be successfully transferred and might result in live births. Such results might raise awareness that discarding embryos based only on early events is not a suitable approach to give patients the chance to conceive. In conclusion, to date only the transfer of viable embryos after culturing them until day 5 guarantees optimal embryo selection and helps to prevent embryo wastage.
Assuntos
Blastocisto , Transferência Embrionária , Oócitos , Adulto , Técnicas de Cultura Embrionária , Implantação do Embrião , Retículo Endoplasmático Liso , Feminino , Humanos , Masculino , Oócitos/citologia , Gravidez , Estudos Retrospectivos , Imagem com Lapso de Tempo , Transferência Intratubária do ZigotoAssuntos
Gastroenteropatias/fisiopatologia , Infecções/fisiopatologia , Oócitos/fisiologia , Taxa de Gravidez , Adulto , Blastocisto/fisiologia , Diarreia/microbiologia , Diarreia/fisiopatologia , Feminino , Fertilização in vitro/métodos , Gastroenteropatias/microbiologia , Humanos , Infecções/microbiologia , Oócitos/patologia , GravidezRESUMO
Spermatozoa selection at high magnification before intracytoplasmic sperm injection seems to be positively associated with pregnancy rates after day 3 embryo transfers. The aim was to demonstrate an association between the presence of vacuoles in sperm nuclei and the competence of embryos to develop to day 5. Grading of spermatozoa at x 6000-x 12,500 magnification: grade I, no vacuoles; grade II,