Original Method for Evaluating Endothelial Vasomotor Function of Isolated Vessel in Experiment.

We describe a method for experimental assessment of endothelial dysfunction in an isolated using extracorporeal machine perfusion and vasoactive substrates agents (norepinephrine and acetylcholine). Quantitative assessment of endothelial vasomotor dysfunction was proposed, based on registration of changes in volumetric perfusate flow rate through the isolated vessel. Functional activity of the isolated aorta endothelium and total content of stable NO degradation products in blood plasma were studied in outbred white male rats after subtotal liver resection. Impaired vasodilation ability of the aorta and increased content of NO degradation products in blood plasma observed after surgery indicate the development of vasomotor endothelial dysfunction associated with oxidative stress, cholemia, and endotoxemia.

[Effect of early post-resuscitation endotoxemia on the myocardial function]. / Vliianie rannei postreanimatsionnoi éndotoksemii na funktsiiu miokarda.

The impact made by serum (which was obtained in 30 minutes after resuscitation) on the cardiovascular system was studied in experiments with the total body as well as with isolated and isovolumetrically contracting hearts of white outbred male rats. A 5-minute clinical death was provoked by a severe blood loss. The "toxic" serum, when administered to the intact animals with the macrophage system blocked by trypan blue, was found to reduce the stroke and minute hearts volumes and to increase the general resistance of peripheral vessels. Lower force and velocity indices of the myocardium contractility, a higher consumption of glucose by the heart per a unit of a performed function, disassociation of the oxidation phosphorylation, activation of anaerobic glycolysis and increased discharges of cardiachystiocyte, lactate, pyruvate and of enzymes of different ultrasound localizations were detected in perfusion of the hearts isolated by Krebs-Henzelight solution containing the "toxic" serum.

[Cardioprotective effect of exogenous creatine phosphate in acute hemorrhage]. / Kardioprotektornoe deistvie ékzogennogo kreatinfosfata pri ostroi krovopotere.

Experiments on noninbred albino male rats under the conditions of the whole body and the isolated isovolumically contracting heart have shown that a substantial contribution to postresuscitative cardiac damages is made by energy deficiency that triggers a group of mechanisms of damaging cardiomyocytic membranes. Exogenous creatine phosphate (CP) reduces postresuscitative mortality rates, improves bioenergy, contractile and rhythmical functions of the heart, decreases the rate of myocardial lipid peroxidation, by showing as a whole a cardioprotective action. The latter is realized by directly protecting the cardiomyocytic sarcolemma and the improvement of energy metabolism is secondary. Despite the likely mediation of this effect, improved energy metabolism is undoubtedly a key point of the protective action of exogenous CP if the heart is postresuscitatively damaged.

[Role of disorders of metabolism in rat myocardium and endotoxemia in the pathogenesis of post-resuscitation cardiodepression]. / Rol' narushenii metabolizma v miokarde krys i éndotoksemii v patogeneze postreanimatsionnoi kardiodepressii.

The model of 4-min clinical death due to acute blood loss was used to study cardiac contractility, myocardial metabolism and causes of endotoxemia in early postresuscitation period. The investigations were made on the whole body, isolated, isovolemically contracting heart and isolated papillary muscle. A marked reduction in functional myocardial reserves, maximal within the first 24 hours of postresuscitation, with dominant defects in relaxation was seen. Pathogenetic factors responsible for cardiodepression are the following: hypoxia, impairment of bioenergetics, hyperactivation of lipid peroxidation, acidosis, membrane destruction, endotoxemia.