Functional effects of drugs and toxins interacting with NaV1.4.

NaV1.4 is a voltage-gated sodium channel subtype that is predominantly expressed in skeletal muscle cells. It is essential for producing action potentials and stimulating muscle contraction, and mutations in NaV1.4 can cause various muscle disorders. The discovery of the cryo-EM structure of NaV1.4 in complex with ß1 has opened new possibilities for designing drugs and toxins that target NaV1.4. In this review, we summarize the current understanding of channelopathies, the binding sites and functions of chemicals including medicine and toxins that interact with NaV1.4. These substances could be considered novel candidate compounds or tools to develop more potent and selective drugs targeting NaV1.4. Therefore, studying NaV1.4 pharmacology is both theoretically and practically meaningful.

The Evolution, Expression Patterns, and Domestication Selection Analysis of the Annexin Gene Family in the Barley Pan-Genome.

Plant annexins constitute a conserved protein family that plays crucial roles in regulating plant growth and development, as well as in responses to both biotic and abiotic stresses. In this study, a total of 144 annexin genes were identified in the barley pan-genome, comprising 12 reference genomes, including cultivated barley, landraces, and wild barley. Their chromosomal locations, physical-chemical characteristics, gene structures, conserved domains, and subcellular localizations were systematically analyzed to reveal the certain differences between wild and cultivated populations. Through a cis-acting element analysis, co-expression network, and large-scale transcriptome analysis, their involvement in growth, development, and responses to various stressors was highlighted. It is worth noting that HvMOREXann5 is only expressed in pistils and anthers, indicating its crucial role in reproductive development. Based on the resequencing data from 282 barley accessions worldwide, genetic variations in thefamily were investigated, and the results showed that 5 out of the 12 identified HvMOREXanns were affected by selection pressure. Genetic diversity and haplotype frequency showed notable reductions between wild and domesticated barley, suggesting that a genetic bottleneck occurred on the annexin family during the barley domestication process. Finally, qRT-PCR analysis confirmed the up-regulation of HvMOREXann7 under drought stress, along with significant differences between wild accessions and varieties. This study provides some insights into the genome organization and genetic characteristics of the annexin gene family in barley at the pan-genome level, which will contribute to better understanding its evolution and function in barley and other crops.

The Inhibitory Effect of Magnolol on the Human TWIK1 Channel Is Related to G229 and T225 Sites.

TWIK1 (K2P1.1/KCNK1) belongs to the potassium channels of the two-pore domain. Its current is very small and difficult to measure. In this work, we used a 100 mM NH4+ extracellular solution to increase TWIK1 current in its stable cell line expressed in HEK293. Then, the inhibition of magnolol on TWIK1 was observed via a whole-cell patch clamp experiment, and it was found that magnolol had a significant inhibitory effect on TWIK1 (IC50 = 6.21 ± 0.13 µM). By molecular docking and alanine scanning mutagenesis, the IC50 of TWIK1 mutants G229A, T225A, I140A, L223A, and S224A was 20.77 ± 3.20, 21.81 ± 7.93, 10.22 ± 1.07, 9.55 ± 1.62, and 7.43 ± 3.20 µM, respectively. Thus, we conclude that the inhibition of the TWIK1 channel by magnolol is related to G229 and T225 on the P2- pore helix.

Differential dose-response patterns of intracellular and extracellular antibiotic resistance genes under sub-lethal antibiotic exposure.

Although antibiotics are one of the most significant factors contributing to the propagation of antibiotic resistance genes (ARGs), studies on the dose-response relationship at sub-lethal concentrations of antibiotics remain scarce, despite their importance for assessing the risks of antibiotics in the environment. In this study, we constructed a series of microcosms to investigate the propagation of intracellular (iARGs) and extracellular (eARGs) ARGs in both water and biofilms when exposed to antibiotics at various concentrations (1-100 µg/L) and frequencies. Results showed that eARGs were more abundant than iARGs in water, while iARGs were the dominant ARGs form in biofilms. eARGs showed differentiated dose-response relationships from iARGs. The abundance of iARGs increased with the concentration of antibiotics as enhanced selective pressure overcame the metabolic burden of antibiotic-resistant bacteria carrying ARGs. However, the abundance of eARGs decreased with increasing antibiotic concentrations because less ARGs were secreted from bacterial hosts at higher concentrations (100 µg/L). Furthermore, combined exposure to two antibiotics (tetracycline & imipenem) showed a synergistic effect on the propagation of iARGs, but an antagonistic effect on the propagation of eARGs compared to exposure to a single antibiotic. When exposed to antibiotic at a fixed total dose, one-time dosing (1 time/10 d) favored the propagation of iARGs, while fractional dosing (5 times /10 d) favored the propagation of eARGs. This study sheds light on the propagation of antibiotic resistance in the environment and can help in assessing the risks associated with the use of antibiotics.

SNPD-CRISPR: Single Nucleotide Polymorphism-Distinguishable Repression or Enhancement of a Target Gene Expression by CRISPR System.

A wide range of diseases, including cancer, autoimmune diseases, or neurodegenerative diseases, have been associated with single nucleotide mutations in their causative genes. Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is a flexible and efficient genome engineering technology widely used for researches and therapeutic applications which offers immense opportunity to treat genetic diseases. The complex of Cas9 and the guide RNA acts as an RNA-guided endonuclease. Cas9 recognizes a sequence motif known as a protospacer adjacent motif (PAM), and then the guide RNA base pairs with its proximal target region of 20 nucleotides with sequence complementarity. Here we describe the procedure named single nucleotide polymorphism-distinguishable (SNPD)-CRISPR system which can suppress or enhance the expression of disease-causative gene with single nucleotide mutation distinguished from its wild-type. In this study, we used HRAS, one of most famous cancer-causative genes, as an example of a target gene.

Quantitatively Visualizing the Thermal Dehydration Process and Isotope Effect in Single HKUST-1 Metal-Organic Framework Particles.

Quantitatively visualizing the thermal dehydration in metal-organic frameworks (MOFs), especially at the single-particle level, is still challenging, hindering a deeper understanding of the reaction dynamics. Using in situ dark-field microscopy (DFM), we image the thermal dehydration process of single water-containing HKUST-1 (H2O-HKUST-1) metal-organic framework (MOF) particles. DFM maps the color intensity of single H2O-HKUST-1, which is linearly correlated with the water content in the HKUST-1 framework, enabling a direct quantification of several reaction kinetic parameters of single HKUST-1 particles. Interestingly, when H2O-HKUST-1 is transformed into deutoxide (D2O)-containing HKUST-1, the corresponding thermal dehydration reaction displays higher temperature parameters and activation energy but shows a lower rate constant and diffusion coefficient, revealing the isotope effect. The significant variation of the diffusion coefficient is also confirmed by molecular dynamics simulations. The present operando results are anticipated to provide valuable guidelines for the design and development of advanced porous materials.

Spatial Cognition of the Visually Impaired: A Case Study in a Familiar Environment.

OBJECTIVES: This paper aims to explore the factors influencing the spatial cognition of the visually impaired in familiar environments. BACKGROUND: Massage hospitals are some of the few places that can provide work for the visually impaired in China. Studying the spatial cognition of the visually impaired in a massage hospital could be instructive for the design of working environments for the visually impaired and other workplaces in the future. METHODS: First, the subjective spatial cognition of the visually impaired was evaluated by object layout tasks for describing the spatial relationships among object parts. Second, physiological monitoring signal data, including the electrodermal activity, heart rate variability, and electroencephalography, were collected while the visually impaired doctors walked along prescribed routes based on the feature analysis of the physical environment in the hospital, and then their physiological monitoring signal data for each route were compared. The visual factors, physical environmental factors, and human-environment interactive factors that significantly impact the spatial cognition of visually impaired people were discussed. CONCLUSIONS: (1) visual acuity affects the spatial cognition of the visually impaired in familiar environments; (2) the spatial cognition of the visually impaired can be promoted by a longer staying time and the more regular sequence of a physical environment; (3) the spatial comfort of the visually impaired can be improved by increasing the amount of greenery; and (4) the visual comfort of the visually impaired can be reduced by rich interior colors and contrasting lattice floor tiles.

Surface Curvature Dominated Guest-Induced Nonequilibrium Deformations of Single Covalent Organic Framework-300 Particles.

Understanding the guest-induced dynamic deformation process of covalent organic frameworks (COFs) is vitally important to further increase their stimulus-response performances. Here we report on the dark-field microscopic (DFM) imaging approach to in situ monitor the guest-induced deformation evolution of individual COF-300 crystals in real time. We observe not only transient and nonequilibrium intermediate deformation states but also local surface curvature-driven diverse adsorption behaviours of single COF-300 particles for dichloromethane (DCM), undergoing one, two, and multiple expansion-contraction deformations as well as contraction-to-expansion transition. The surface curvature-dominated deformations are ascribed to the significant differences in the adsorption capacity for DCM at the curved tip and flat side regions, in which DCM can be adsorbed preferentially by curved tip regions of COF-300.

A feasibility investigation of a pilot-scale bioelectrochemical coupled anaerobic digestion system with centric electrode module for real membrane manufacturing wastewater treatment.

The large-scale application of bioelectrochemical coupled anaerobic digestion (BES-AD) is limited by the matching of electrode configuration and the applicability of real wastewater. In this study, a pilot-scale BES-AD system with an effective system volume of 5 m3 and a 1 m3 volume of a carbon fiber brush electrode module was constructed and tested for treatment of the membrane manufacturing wastewater. The results showed that the BOD5/COD of the wastewater was increased from 0.238 to 0.398 when the applied voltage was 0.9 V. The pollutants such as N, N-Dimethylacetamide and glycerol in wastewater were degraded significantly. The microorganisms in the electrode modules were spatially enriched. The fermenters (Norank_f__ML635J-40_aquatic_group, 6.55 %; unclassified_f__Propionibacteriaceae, 5.25 %) and degraders (Corynebacterium, 29.31 %) were mostly enriched at the bottom, while electroactive bacteria (Pseudomonas, 29.39 %, Geobacter, 7.86 %) were mostly enriched at the top. Combined with the economical construction and operation cost ($1708.8/m3 and $0.76/m3) of the BES-AD system.

The microbial synergy and response mechanisms of hydrolysis-acidification combined microbial electrolysis cell system with stainless-steel cathode for textile-dyeing wastewater treatment.

Microbial electrolysis cell (MEC) has been existing problems such as poor applicability to real wastewater and lack of cost-effective electrode materials in the practical application of refractory wastewater. A hydrolysis-acidification combined MEC system (HAR-MECs) with four inexpensive stainless-steel and conventional carbon cloth cathodes for the treatment of real textile-dyeing wastewater, which was fully evaluated the technical feasibility in terms of parameter optimization, spectral analysis, succession and cooperative/competition effect of microbial. Results showed that the optimum performance was achieved with a 12 h hydraulic retention time (HRT) and an applied voltage of 0.7 V in the HAR-MEC system with a 100 µm aperture stainless-steel mesh cathode (SSM-100 µm), and the associated optimum BOD5/COD improvement efficiency (74.75 ± 4.32 %) and current density (5.94 ± 0.03 A·m-2) were increased by 30.36 % and 22.36 % compared to a conventional carbon cloth cathode. The optimal system had effective removal of refractory organics and produced small molecules by electrical stimulation. The HAR segment could greatly alleviate the imbalance between electron donors and electron acceptors in the real refractory wastewater and reduce the treatment difficulty of the MEC segment, while the MEC system improved wastewater biodegradability, amplified the positive and specific interactions between degraders, fermenters and electroactive bacteria due to the substrate complexity. The SSM-100 µm-based system constructed by phylogenetic molecular ecological network (pMEN) exhibited moderate complexity and significantly strong positive correlation between electroactive bacteria and fermenters. It is highly feasible to use HAR-MEC with inexpensive stainless-steel cathode for textile-dyeing wastewater treatment.

Promising therapeutic aspects in human genetic imprinting disorders.

Genomic imprinting is an epigenetic phenomenon of monoallelic gene expression pattern depending on parental origin. In humans, congenital imprinting disruptions resulting from genetic or epigenetic mechanisms can cause a group of diseases known as genetic imprinting disorders (IDs). Genetic IDs involve several distinct syndromes sharing homologies in terms of genetic etiologies and phenotypic features. However, the molecular pathogenesis of genetic IDs is complex and remains largely uncharacterized, resulting in a lack of effective therapeutic approaches for patients. In this review, we begin with an overview of the genomic and epigenomic molecular basis of human genetic IDs. Notably, we address ethical aspects as a priority of employing emerging techniques for therapeutic applications in human IDs. With a particular focus, we delineate the current field of emerging therapeutics for genetic IDs. We briefly summarize novel symptomatic drugs and highlight the key milestones of new techniques and therapeutic programs as they stand today which can offer highly promising disease-modifying interventions for genetic IDs accompanied by various challenges.

Dysregulated adipose tissue expansion and impaired adipogenesis in Prader-Willi syndrome children before obesity-onset.

OBJECTIVE: Prader-Willi syndrome (PWS) is a rare genetic imprinting disorder resulting from the expression loss of genes on the paternally inherited chromosome 15q11-13. Early-onset life-thriving obesity and hyperphagia represent the clinical hallmarks of PWS. The noncoding RNA gene SNORD116 within the minimal PWS genetic lesion plays a critical role in the pathogenesis of the syndrome. Despite advancements in understanding the genetic basis for PWS, the pathophysiology of obesity development in PWS remains largely uncharacterized. Here, we aimed to investigate the signatures of adipose tissue development and expansion pathways and associated adipose biology in PWS children without obesity-onset at an early stage, mainly from the perspective of the adipogenesis process, and further elucidate the underlying molecular mechanisms. METHODS: We collected inguinal (subcutaneous) white adipose tissues (ingWATs) from phase 1 PWS and healthy children with normal weight aged from 6 M to 2 Y. Adipose morphology and histological characteristics were assessed. Primary adipose stromal vascular fractions (SVFs) were isolated, cultured in vitro, and used to determine the capacity and function of white and beige adipogenic differentiation. High-throughput RNA-sequencing (RNA-seq) was performed in adipose-derived mesenchymal stem cells (AdMSCs) to analyze transcriptome signatures in PWS subjects. Transient repression of SNORD116 was conducted to evaluate its functional relevance in adipogenesis. The changes in alternative pre-mRNA splicing were investigated in PWS and SNORD116 deficient cells. RESULTS: In phase 1 PWS children, impaired white adipose tissue (WAT) development and unusual fat expansion occurred long before obesity onset, which was characterized by the massive enlargement of adipocytes accompanied by increased apoptosis. White and beige adipogenesis programs were impaired and differentiated adipocyte functions were disturbed in PWS-derived SVFs, despite increased proliferation capacity, which were consistent with the results of RNA-seq analysis of PWS AdMSCs. We also experimentally validated disrupted beige adipogenesis in adipocytes with transient SNORD116 downregulation. The transcript and protein levels of PPARγ, the adipogenesis master regulator, were significantly lower in PWS than in control AdMSCs as well as in SNORD116 deficient AdMSCs/adipocytes than in scramble (Scr) cells, resulting in the inhibited adipogenic program. Additionally, through RNA-seq, we observed aberrant transcriptome-wide alterations in alternative RNA splicing patterns in PWS cells mediated by SNORD116 loss and specifically identified a changed PRDM16 gene splicing profile in vitro. CONCLUSIONS: Imbalance in the WAT expansion pathway and developmental disruption are primary defects in PWS displaying aberrant adipocyte hypertrophy and impaired adipogenesis process, in which SNORD116 deficiency plays a part. Our findings suggest that dysregulated adiposity specificity existing at an early phase is a potential pathological mechanism exacerbating hyperphagic obesity onset in PWS. This mechanistic evidence on adipose biology in young PWS patients expands knowledge regarding the pathogenesis of PWS obesity and may aid in developing a new therapeutic strategy targeting disturbed adipogenesis and driving AT plasticity to combat abnormal adiposity and associated metabolic disorders for PWS patients.

Combination of variational mode decomposition and coherent factor for ultrasound computer tomography.

BACKGROUND: Ultrasound computed tomography (USCT) is a promising technique for improving the detection of breast cancer. Image quality of USCT has a major impact on the breast cancer diagnosis. OBJECTIVE: This paper investigates the combination of variational mode decomposition (VMD) and coherent factor method for USCT image quality enhancement. METHODS: The signals can be decomposed into multiple intrinsic mode functions (IMFs) sifting through the frequency by VMD method. Refactoring the remaining IMFs, spatio-temporally smoothed coherence factor (STSCF) beamforming method is applied to reconstructed data for USCT. RESULTS: The validation of combination the VMD and STSCF is described through the breast phantom experiment and in vivo experiments. The evaluation indicators such as contrast ratio (CR), contrast to noise ratio (CNR) and signal to noise ratio (SNR) have been better improved in the experimental results. For the breast phantom, the proposed method gives a higher resolution and the better contrast properties for the hyperechoic cyst. The borders of cysts and tumors in the breast phantom can be distinguished clearly. For volunteer breast experiments, artifacts are removed more efficiently while the clutters are suppressed simultaneously. CONCLUSION: The combination of VMD and STSCF can further reduce the noise and suppress the side lobes.

CircZNF652 promotes the goblet cell metaplasia by targeting the miR-452-5p/JAK2 signaling pathway in allergic airway epithelia.

BACKGROUND: Circular RNAs (circRNAs) play potentially important roles in various human diseases; however, their roles in the goblet cell metaplasia of asthma remain unknown. OBJECTIVE: We sought to investigate the potential role and underlying mechanism of circZNF652 in the regulation of allergic airway epithelial remodeling. METHODS: The differential expression profiles of circRNAs were analyzed by transcriptome microarray, and the effects and mechanisms underlying circZNF652-mediated goblet cell metaplasia were investigated by quantitative real-time PCR, RNA fluorescence in situ hybridization, Western blot, RNA pull-down, and RNA immunoprecipitation analyses. The roles of circZNF652 and miR-452-5p in allergic airway epithelial remodeling were explored in both the mouse model with allergic airway inflammation and children with asthma. RESULTS: One hundred sixty circRNAs were differentially expressed in bronchoalveolar lavage fluid of children with asthma versus children with foreign body aspiration, and 52 and 108 of them were significantly upregulated and downregulated, respectively. Among them, circZNF652 was predominantly expressed and robustly upregulated in airway epithelia of both the children with asthma and the mouse model with allergic airway inflammation. circZNF652 promoted the goblet cell metaplasia by functioning as a sponge of miR-452-5p, which released the Janus kinase 2 (JAK2) expression and subsequently activated JAK2/signal transducer and activator of transcription 6 (STAT6) signaling in the allergic airway epithelia. In addition, epithelial splicing regulatory protein 1, a splicing factor, accelerated the biogenesis of circZNF652 by binding to its flanking intron to promote the goblet cell metaplasia in allergic airway epithelial remodeling. CONCLUSIONS: Upregulation of circZNF652 expression in allergic bronchial epithelia contributed to the goblet cell metaplasia by activating the miR-452-5p/JAK2/STAT6 signaling pathway; thus, blockage of circZNF652 or agonism of miR-452-5p provided an alternative approach for the therapeutic intervention of epithelial remodeling in allergic airway inflammation.

Bioelectrochemical systems with a cathode of stainless-steel electrode for treatment of refractory wastewater: Influence of electrode material on system performance and microbial community.

The large-scale application of the bioelectrochemical system (BES) is limited by the cost-effective electrode materials. In this study, five kinds of stainless-steel materials were used as the cathode of the BES coupled with anaerobic digestion (BES-AD) for the treatment of diluted N, N-dimethylacetamide (DMAC) wastewater. Compared with a carbon-cloth cathode, BES-AD with a stainless-steel cathode had more engineering due to its low cost, although the operating efficiencies were slightly inferior. Stainless-steel mesh with a 100 µm aperture (SSM-100 µm) was the most cost-effective electrode and the implanted BES exhibited better COD removal efficiency, electrochemical performance and biodegradability. Analysis of microbial community revealed the synergetic effect between exoelectrogen and fermentative bacteria had been strengthened in the SSM-100 µm cathode biofilm. Function analysis of the microbial community based on PICRUSt predicted metagenomes revealed that the metabolic pathways of xenobiotics biodegradation and metabolism in the SSM-100 µm cathode were stimulated.

Visual colorimetric detection of triacetone triperoxide based on a Fe(II)-promoted thermal decomposition process.

Triacetone triperoxide (TATP) explosives, a popular choice for terrorists, have been used in many violent terrorist attacks all over the world. However, simple, rapid, and on-site detection methods of TATP are still lacking. Herein, we present a visual colorimetric method for on-site and rapid detection of TATP based on a Fe(II)-promoted thermal decomposition process of TATP. We discovered that TATP can be decomposed into H2O2 under heating conditions, and it reacts with Fe2+ to produce hydroxyl radicals (˙OH) and Fe3+via the Fenton reaction. The resulting ˙OH and Fe3+ further oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to a yellow oxidized product (oxTMB). These reaction processes remarkably promote the chemical equilibrium shift and decrease the activation energy. Using the TATP-Fe2+-TMB ternary chromogenic system, the present colorimetric assay for TATP shows a dynamic range of 0.5-30 µM with a low detection limit of 0.12 µM. Additionally, common substances (e.g., inorganic salts, small organic substances, and polymers) do not interfere with TATP detection. This assay can be used for analyzing TATP in real water and camouflage samples. Furthermore, a test-paper-based method was also successfully developed for visual, rapid and on-site detection of TATP.

Combining electrochemical nitrate reduction and anammox for treatment of nitrate-rich wastewater: A short review.

Treatment of nitrate-rich wastewater is important but challenging for the conventional biological denitrification process. Here, we propose combining the electrochemical reduction and anaerobic ammonium oxidation (anammox) processes together for treatment of nitrate-rich wastewater. This article reviews the mechanism and current research status of electrochemical reduction of nitrate to ammonium as well as the mechanism and applicability of the anammox process. This article discusses the principles, superiorities and challenges of this combined process. The feasibility of the combined process depends on the efficiency of electrochemical nitrate reduction to ammonium and the conditions in the anammox process to use the reduced ammonium as the substrate to achieve deep nitrogen removal. The article provides a feasible strategy for using the electrochemical reduction and anammox combined process to treat nitrate-rich wastewater.

Multi-Scale Modeling of Microstructure Evolution during Multi-Pass Hot-Rolling and Cooling Process.

In this work, a 6-pass hot-rolling process followed by air cooling is studied by means of a coupled multi-scale simulation approach. The finite element method (FEM) is utilized to obtain macroscale thermomechanical parameters including temperature and strain rate. The microstructure evolution during the recrystallization and austenite (γ) to ferrite (α) transformation is simulated by a mesoscale cellular automaton (CA) model. The solute drag effect is included in the CA model to take into account the influence of manganese on the γ/α interface migration. The driving force for α-phase nucleation and growth also involves the contribution of the deformation stored energy inherited from hot-rolling. The simulation renders a clear visualization of the evolving grain structure during a multi-pass hot-rolling process. The variations of the nonuniform, deformation-stored energy field and carbon concentration field are also reproduced. A detailed analysis demonstrates how the parameters, including strain rate, grain size, temperature, and inter-pass time, influence the different mechanisms of recrystallization. Grain refinement induced by recrystallization and the γ→α phase transformation is also quantified. The simulated final α-fraction and the average α-grain size agree reasonably well with the experimental microstructure.

ß-aminobutyric acid induces priming defence against Botrytis cinerea in grapefruit by reducing intercellular redox status that modifies posttranslation of VvNPR1 and its interaction with VvTGA1.

Either NPR1 or TGA1 serve as master redox-sensitive transcriptional regulators for the transcription of PR genes in plants. The redox modification of the two co-activators involved in BABA-induced priming resistance against Botrytis cinerea in grapes was examined in this study. The results showed that 10 mmol L-1 BABA could effectively trigger a priming defense in grapes as manifested by augmented expression levels of PR genes upon inoculation with B. cinerea. Moreover, transcriptome profiling analysis revealed that all of the sets of key genes in the enzymatic ROS scavenging system, the PPP and AsA-GSH cycle were in harmony and were transcriptionally induced in BABA-primed grapes with pathogenic infection; in addition, this enhanced expression caused the accelerated accumulation of reductive substances, namely, AsA, GSH and NADPH, resulting in reduced intercellular conditions. Under reduced conditions, the interaction of VvTGA1 and VvNPR1 in the Y2H assay implied that VvTGA1 can provide the DNA binding capacity required by VvNPR1 for activation of VvPR genes. Consequently, the transactivation of VvNPR1 by the promoters of VvPR1, VvPR2 and VvPR5 was determined via a DLR assay, and it induced the transcription of the VvPR genes. In parallel, the redox-modified reducing condition achieved with an abundant supply of reductive substances was closely associated with the translocation of NPR1 for interaction with TGA in the nucleus. Thus, the posttranslational modification and subsequent interaction of the two redox-sensitive co-activators of VvNPR1 and VvTGA1 under reduced conditions may be responsible for BABA-induced priming for effective disease resistance in grapes.

Comparative analysis of surface water quality prediction performance and identification of key water parameters using different machine learning models based on big data.

The water quality prediction performance of machine learning models may be not only dependent on the models, but also dependent on the parameters in data set chosen for training the learning models. Moreover, the key water parameters should also be identified by the learning models, in order to further reduce prediction costs and improve prediction efficiency. Here we endeavored for the first time to compare the water quality prediction performance of 10 learning models (7 traditional and 3 ensemble models) using big data (33,612 observations) from the major rivers and lakes in China from 2012 to 2018, based on the precision, recall, F1-score, weighted F1-score, and explore the potential key water parameters for future model prediction. Our results showed that the bigger data could improve the performance of learning models in prediction of water quality. Compared to other 7 models, decision tree (DT), random forest (RF) and deep cascade forest (DCF) trained by data sets of pH, DO, CODMn, and NH3-N had significantly better performance in prediction of all 6 Levels of water quality recommended by Chinese government. Moreover, two key water parameter sets (DO, CODMn, and NH3-N; CODMn, and NH3-N) were identified and validated by DT, RF and DCF to be high specificities for perdition water quality. Therefore, DT, RF and DCF with selected key water parameters could be prioritized for future water quality monitoring and providing timely water quality warning.