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Cadmium (Cd) pollution significantly reduces agricultural crop yields. In our research, metabolomic changes in Citrus maxima L. subjected to Cd stress were investigated using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) in tandem with multivariate analytical techniques. This integrative method, coupled with physiological evaluations, aimed to elucidate the core adaptive mechanisms to Cd stress. We found that under Cd stress, C. maxima seedlings exhibited elevated levels of reactive oxygen species, malondialdehyde, and electrolyte leakage. Furthermore, principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) demonstrated distinct a separation of the metabolome among the different treatment groups under Cd stress, indicating dynamic metabolic changes. Metabolic analysis suggested that genes involved are initially induced by Cd treatment, followed by the activation of the flavonoid biosynthesis pathway. This investigation provides new insights into the complex metabolic responses of C. maxima seedlings to Cd exposure.
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Cádmio , Citrus , Plântula , Citrus/metabolismo , Citrus/efeitos dos fármacos , Citrus/genética , Cádmio/toxicidade , Cádmio/metabolismo , Plântula/metabolismo , Plântula/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Metaboloma/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacos , Metabolômica/métodos , Malondialdeído/metabolismo , Espectrometria de Massas/métodos , Espécies Reativas de Oxigênio/metabolismo , Análise de Componente PrincipalRESUMO
Propolis exhibits significant anti-inflammatory, antidiabetic, and antiobesity properties in both mouse models and clinical applications. However, the underlying metabolic mechanisms remain poorly understood. Traditional metabolomic methods that rely on chromatographic separation require complex preprocessing steps and extended detection periods. In this study, we employed extraction electrospray ionization mass spectrometry combined with multivariate analysis to directly profile metabolites in the colon tissue of mice. Our findings demonstrate the efficacy of ethanol extract of propolis (EEP) in mitigating weight gain, reducing inflammatory cytokines, and improving insulin resistance induced by a high-fat diet. Additionally, EEP enhanced glucose tolerance. Through collision-induced dissociation experiments, we identified 26 metabolites, with 4-hydroxyphenylacetic acid, protocatechuic acid, caffeic acid, ferulic acid, hippuric acid, histidine, and tryptophan emerging as potential biomarkers. Notably, tryptophan exhibited the highest content at 8.25 mg/g. Our research facilitates rapid profiling of colon metabolites, underscoring its significant potential for broader applications in animal metabolomic studies.
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Colo , Dieta Hiperlipídica , Metabolômica , Camundongos Endogâmicos C57BL , Própole , Espectrometria de Massas por Ionização por Electrospray , Animais , Camundongos , Dieta Hiperlipídica/efeitos adversos , Masculino , Espectrometria de Massas por Ionização por Electrospray/métodos , Colo/metabolismo , Própole/química , Própole/metabolismo , Humanos , Resistência à Insulina , Obesidade/metabolismo , Obesidade/dietoterapiaRESUMO
Pomacea canaliculata, the invasive snail, is a host of the parasitic nematode Angiostrongylus cantonensis, which has adverse effects on the agriculture system and human health. This work evaluated the molluscicidal activity of petroleum ether extracts (PEEs) from three species of Chimonanthus against the snail P. canaliculate. Pcp (PEE of C. praecox) showed the most effective molluscicide activity. Sixty-one compounds were identified by GC-MS and the main components were terpenoids and fatty acids. The half-lethal concentration (LC50) of Pcp at 24 h (0.27 mg/mL) and 48 h (0.19 mg/mL) was used to evaluate the biochemical alterations in snail tissue. These sublethal concentrations caused the levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activity to increase, while acetylcholinesterase (AChE) activity decreased. Also, under LC50 treatment, several histological changes were observed in the hepatopancreas and foot of the snail compared with the control group. Moreover, the toxic test in rice demonstrated that Pcp has low toxicity. These results suggest that Pcp could be developed as an effective molluscicide for P. canaliculata control.
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Moluscocidas , Extratos Vegetais , Folhas de Planta , Caramujos , Animais , Moluscocidas/farmacologia , Moluscocidas/química , Extratos Vegetais/farmacologia , Extratos Vegetais/química , Caramujos/efeitos dos fármacos , Folhas de Planta/químicaRESUMO
The ultrasonic-assisted extraction (UAE) method was employed to separate Cinnamomum camphora proanthocyanidin-rich extracts (PCEs). This extraction process was optimized by the Box-Behnken design, and the optimal conditions, on a laboratory scale, were as follows: an ethanol concentration of 75%, a liquid-to-solid ratio of 24 mL/g, an ultrasonic time of 39 min, and an ultrasonic power of 540 W. Under the obtained conditions, the PCE yield extracted by UAE was higher than that from heat reflux extraction and soaking extraction. An ultra-performance liquid chromatography-tandem mass spectrometry analysis was employed to characterize the phloroglucinolysis products of the C. camphora PCEs, by which epigallocatechin, catechin, epicatechin, and (-)-epigallocatechin-3-O-gallate were identified as the terminal units; epigallocatechin, epicatechin, and (-)-epigallocatechin-3-O-gallate were recognized as extension units. The C. camphora PCEs possessed higher anti-ultraviolet activity in vitro compared with the commercially available sunscreen additive of benzophenone with respect to their ethanol solutions (sun protection factor of 27.01 ± 0.68 versus 1.96 ± 0.07 at a concentration of 0.09 mg/mL) and sunscreens (sun protection factor of 17.36 ± 0.62 versus 14.55 ± 0.47 at a concentration of 20%). These results demonstrate that C. camphora PCEs possess an excellent ultraviolet-protection ability and are promising green sunscreen additives that can replace commercial additives.
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Catequina , Cinnamomum camphora , Proantocianidinas , Ultrassom , Protetores Solares , Etanol/química , Extratos Vegetais/farmacologia , Extratos Vegetais/químicaRESUMO
Acute myeloid leukemia (AML) is a form of blood cancer that arise as a result of clonal proliferation of malignant myeloid precursors acquiring genetic abnormalities. Primary resistance to initial treatment and disease recurrence continues to be huge challenge in treating AML. Herein, GSE114868 was analyzed for differentially-expressed lncRNAs between AML patients' mononucleated cells and healthy normal control mononucleated cells and 191 lncRNAs were significantly deregulated in AML patients' mononucleated cells. The correlation between candidate lncRNAs and AML patients' overall survival was analyzed and 6 lncRNAs, including MIR181A1HG, TRAF3IP2-AS1, STARD4-AS1, E2F3-IT1, FAM215A, and HHIP-AS1 were dramatically linked to AML patients' OS. Using a Cox proportional-hazards model, we identified risk factors and found FAM215A as a risk factor for AML patients' prognosis. The expression level of FAM215A showed to be upregulated within blood samples and cells. Genes correlated with FAM215A were correlated to cell division, modulation of cell apoptosis, and modulation of programmed cell death. FAM215A knockdown inhibited AML cell viability, elicited G0/G1-phase arrest of cell cycle, enhanced cell apoptosis, increased proapoptotic Bax and cleaved-caspase3 levels, and decreased antiapoptotic Bcl2. FAM215A overexpression exerted opposite effects on AML cells. Conclusively, FAM215A serves as an oncogenic lncRNA in AML, promoting cell viability, relieving cell cycle arrest, and suppressing cell apoptosis. FAM215A might be un underlying biological prognostic marker and therapeutic target for AML.
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Leucemia Mieloide Aguda , RNA Longo não Codificante , Humanos , Biomarcadores , Linhagem Celular Tumoral , Proliferação de Células/genética , Leucemia Mieloide Aguda/genética , Leucemia Mieloide Aguda/metabolismo , Leucemia Mieloide Aguda/patologia , Fenótipo , Prognóstico , RNA Longo não Codificante/genética , RNA Longo não Codificante/metabolismoRESUMO
Low temperatures significantly impact on rice (Oryza sativa) yield and quality. Traditional metabolomic techniques, often involving time-consuming chromatography-mass spectrometry procedures, are currently in use. This study investigated metabolomic responses of rice seedlings under low-temperature stress using nanoliter electrospray ionization mass spectrometry (nanoESI-MS) in combination with multivariate analysis. Results revealed distinct metabolic profiles in 'Qiutianxiaoting' (japonica) and '93-11' (indica) rice seedlings. Among the 36 identified compounds in rice, seven key metabolites, comprising l-glutamic acid, asparagine, tryptophan, citric acid, α-linolenic acid, malic acid, and inositol, were identified as responsive to cold stress. Notably, malic acid content reached 1332.40 µg/g dry weight in Qiutianxiaoting and 1444.13 µg/g in 93-11. Both the qualitative and quantitative results of nanoESI-MS were further confirmed through gas chromatography-mass spectrometry validation. The findings highlight the potential of nanoESI-MS for rapidly characterizing crucial metabolites across diverse plant species under exposure to stress.
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Oryza , Espectrometria de Massas por Ionização por Electrospray , Plântula/metabolismo , Oryza/química , Metabolômica/métodosRESUMO
An aqueous enzymatic-ultrasound cavitation extraction (AEUCE) method was developed to separate Sapium sebiferum seed kernel oil. In this process, neutral proteinase was screened as the propriate enzyme. The Plackett-Burman and Box-Behnken designs were employed to optimize AEUCE. We determined the optimal extraction conditions, producing an oil yield of 84.22 ± 3.17 %. Gas chromatography-mass spectrometry (GC-MS) analysis indicated that the S. sebiferum seed kernel oil was abundant in unsaturated fatty acids (>92 %) and that the compositions of the fatty acid profiles extracted by AEUCE were similar to those obtained from Soxhlet extraction, but their contents were slightly different. The physicochemical properties analysis showed that the oil extracted by AEUCE was comparable to that obtained from Soxhlet extraction. The results showed that the developed AEUCE is an efficient technique that can separate high-quality plant oils. The S. sebiferum seed kernel oil obtained from this extraction method is a promising substitute for vegetable oils used in biodiesel production.
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Euphorbiaceae , Água/química , Óleos de Plantas/química , Sementes/química , Ácidos Graxos/análiseRESUMO
BACKGROUND: Accumulating evidence suggests that members of the tripartite motif (TRIMs) family play a crucial role in the development and progression of hematological malignancy. Here, we explored the expression and potential role of TRIM10 in acute myeloid leukemia (AML). METHODS: The expression levels of TRIM10 were investigated in AML patients and cell lines by RNA-seq, qRT-PCR and Western blotting analysis. Lentiviral infection was used to regulate the level of TRIM10 in AML cells. The effects of TRIM10 on apoptosis, drug sensitivity and proliferation of AML cells were evaluated by flow cytometry and cell-counting kit-8 (CCK-8) assay, as well as being assessed in a murine model. RESULTS: TRIM10 mRNA and protein expression was reduced in primary AML samples and AML cell lines in comparison to the normal controls and a human normal hematopoietic cell line, respectively. Moreover, overexpression of TRIM10 in HL60 and K562 cells inhibited AML cell proliferation and induced cell apoptosis. The nude mice study further confirmed that overexpression of TRIM10 blocked tumor growth and inhibited cell proliferation. In contrast, knockdown of TRIM10 in AML cells showed contrary results. Subsequent mechanistic studies demonstrated that knockdown of TRIM10 enhanced the expression of nuclear protein P65, which implied the activation of the NF-κB signal pathway. Consistently, overexpression of TRIM10 in AML cells showed a contrary result. These data indicated that inactivation of the NF-κB pathway is involved in TRIM10-mediated regulation in AML. TRIM10 expression can be de-repressed by a combination that targets both DNA methyltransferase and histone deacetylase. CONCLUSIONS: Our results strongly suggested that TRIM10 plays a tumor suppressive role in AML development associated with the NF-κB signal pathway and may be a potential target of epigenetic therapy against leukemia.
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Low temperature, a major abiotic stress, often causes molecular changes in crops, which leads to metabolic disturbances and probably affects crop yield. In this study, chilling stress induced distinct metabolic profiles associated with transcriptome regulation, exhibiting great metabolic differences between Qiutianxiaoting (japonica) and 93-11 (indica). In total, 41 and 58 differential metabolites were screened and identified in Qiutianxiaoting and 93-11, respectively. Five key metabolites were screened in response to chilling stress, which were involved or related to different metabolic pathways. Moreover, starch and sucrose metabolism, aminoacyl-tRNA biosynthesis, and phenylpropanoid biosynthesis were significantly enriched in Qiutianxiaoting to maintain cellular homeostasis. Aminoacyl-tRNA biosynthesis and antioxidation metabolism were significantly enriched in 93-11, but disorders of the metabolome and transcriptome occurred at recovery stage. The results could provide some useful information for in-depth understanding of cold-resistant mechanisms, as well as reference for the selection and breeding of rice varieties.
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Oryza , Oryza/química , Melhoramento Vegetal , Metabolômica/métodos , Perfilação da Expressão Gênica , Transcriptoma , Espectrometria de Massas , Cromatografia Líquida , RNA de Transferência/metabolismoRESUMO
The outcome of colon adenocarcinoma (COAD) patients remains dismal, and lactate metabolism has been characterized to promote tumor development and immune evasion. Based on the above background, it is worthwhile to explore novel prognostic and therapeutic biomarkers for COAD patients from the aspect of lactate metabolism. Above all, 228 available lactate-metabolism-related genes (LMRGs) were acquired, and the landscape of copy number variation and the expression difference of mRNA levels between colon normal and tumor samples were investigated among these LMRGs. Importantly, eight overall survival (OS)-involved LMRGs were then distinguished by means of univariate Cox regression analysis in both GSE40967 and TCGA-COAD data sets. Subsequently, prognostic risk scores were established, integrating seven OS-related LMRGs by LASSO Cox regression analysis in the GSE40967 set, and then verified in the TCGA-COAD cohort. From the comprehensive analyses, COAD patients with high risk had comparatively more inferior survival probability in all populations of the study, and they tended to have more severe clinicopathological features with the risk score increasing. Moreover, by integrating age, AJCC T and pathological stage, and risk score, we constructed a prognostic nomogram that demonstrated great prediction effectiveness for OS of COAD patients. Furthermore, the potential effect of various risk score on tumor immune was assessed from enrichment of immune-related pathways, tumor-infiltrating immune cells, and expression levels of immune checkpoints separately. We could draw a conclusion that COAD patients with higher lactate-metabolism-related risk scores may acquire an immunosuppressive tumor microenvironment, which subsequently led to immune escapes and poor prognoses. Conclusively, all findings in the present study illustrate a great prognostic value of the lactate-metabolism-related risk signature, providing more in-depth insights into the indispensable function of lactate metabolism in prognosis and tumor immunity of COAD.
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Amorphophallus konjac, a perennial herb in the Araceae family, is a cash crop that can produce a large amount of konjac glucomannan. To explore mechanisms underlying such large genomes in the genus Amorphophallus as well as the gene regulation of glucomannan biosynthesis, we present a chromosome-level genome assembly of A. konjac with a total genome size of 5.60 Gb and a contig N50 of 1.20 Mb. Comparative genomic analysis reveals that A. konjac has undergone two whole-genome duplication (WGD) events in quick succession. Two recent bursts of transposable elements are identified in the A. konjac genome, which contribute greatly to the large genome size. Our transcriptomic analysis of the developmental corms characterizes key genes involved in the biosynthesis of glucomannan and related starches. High expression of cellulose synthase-like A, Cellulose synthase-like D, mannan-synthesis related 1, GDP-mannose pyrophosphorylase and phosphomannomutase fructokinase contributes to glucomannan synthesis during the corm expansion period while high expression of starch synthase, starch branching enzyme and phosphoglucomutase is responsible for starch synthesis in the late corm development stage. In conclusion, we generate a high-quality genome of A. konjac with different sequencing technologies. The expansion of transposable elements has caused the large genome of this species. And the identified key genes in the glucomannan biosynthesis provide valuable candidates for molecular breeding of this crop in the future.
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Cytochrome P450s are one of the most versatile oxidases that catalyze significant and unique chemical transformations for the construction of complex structural frameworks during natural product biosynthesis. Here, we discovered a set of P450s, including SdnB, SdnH, SdnF, and SdnE, that cooperatively catalyzes the reshaping of the inert cycloaraneosene framework to form a highly oxidized and rearranged sordarinane architecture. Among them, SdnB is confirmed to be the first P450 (or oxidase) that cleaves the C-C bond of the epoxy residue to yield formyl groups in pairs. SdnF selectively oxidizes one generated formyl group to a carboxyl group and accelerates the final Diels-Alder cyclization to furnish the sordarinane architecture. Our work greatly enriches the enzyme functions of the P450 superfamily, supplies the missing skills of the P450 synthetic toolbox, and supports them as biocatalysts in further applications toward the synthesis of new chemical entities.
Assuntos
Sistema Enzimático do Citocromo P-450 , Diterpenos , Catálise , Sistema Enzimático do Citocromo P-450/metabolismo , Oxirredução , Metabolismo SecundárioRESUMO
The fatty acid composition of the kernel of Chimonanthus praecox cv. Luteus (FKC) was analyzed by gas chromatography-mass spectrometry (GC-MS), its ability to kill Pomacea canaliculata was detected, and the degree of damage and physiological and biochemical effects of an FKC soaking treatment on the hepatopancreas tissue of P. canaliculata were evaluated. In total, 16 fatty acids were detected in FKC, among which 13 were qualitatively identified; octadecadienoic acid (56.76%) and palmitic acid (17.03%) had the highest contents. After 48 h of treatment with FKC, the hepatopancreas of P. canaliculata had a large area of necrosis. The contents of soluble sugar, soluble protein, and albumin (Alb) in the hepatopancreas of P. canaliculata decreased with increasing FKC concentration. The content of malondialdehyde (MDA) and the activities of cereal third transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (AKP), and acetylcholinesterase (AChE) increased with increasing FKC concentration. The results showed that FKC has an obvious negative effect on the hepatopancreas cell structure and physiological function of P. canaliculata, i.e., has strong molluscicidal activity.
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Ácidos Graxos , Moluscocidas , Animais , Aspartato Aminotransferases , Hepatopâncreas , CaramujosRESUMO
Herb processing is a typical pharmaceutical preparation process for traditional Chinese medicine. After processing, its clinical applications and pharmacological effects vary greatly, which is most commonly attributed to the changing chemical properties between raw herb and processed products. In this work, a total of 53 chemical compounds were detected, among which 17 compounds were identified as discriminatory chemicals between raw and wine-processed Scutellaria baicalensis, and 10 components were identified as chemical markers with a cumulative content contribution of 88.75%. In addition, this work revealed that the best wine-processed time was 18 min by investigating the changes of chemical markers in S. baicalensis during processing. This work demonstrated that ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry coupled with multiple statistical strategies is an effective approach for screening and identifying discriminatory chemical markers in complex traditional Chinese medicine.
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Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Espectrometria de Massas/métodos , Scutellaria baicalensis/química , Vinho , Biomarcadores/análise , Biomarcadores/química , Medicamentos de Ervas Chinesas/análise , Medicamentos de Ervas Chinesas/isolamento & purificação , Glicosídeos/análise , Glicosídeos/química , Glicosídeos/isolamento & purificação , Medicina Tradicional Chinesa , Análise de Componente PrincipalRESUMO
The activities of ellagic acid in inhibiting mushroom tyrosinase and cell proliferation were evaluated in this research. The results of enzyme kinetics indicated that ellagic acid could effectively inhibit tyrosinase activity. The value of the semi-inhibitory rate (IC50 ) was 0.2 ± 0.05 mM. Ellagic acid inhibited tyrosinase activity in a reversible manner and was a mixed tyrosinase inhibitor. Furthermore, ellagic acid had a good inhibitory effect on the proliferation of mouse melanoma B16 cells and could induce apoptosis. The results acquired from fluorescence spectroscopy revealed that the interaction of ellagic acid with tyrosinase depended on hydrogen bond and electrostatic force. In addition, computational docking showed that ellagic acid interacted with amino acid residues of tyrosinase (Asn19 and Lys372) by hydrogen bond and produced electrostatic interaction with amino residue Lys18. PRACTICAL APPLICATIONS: In the present research, the antityrosinase mechanism of ellagic acid and its effect on mouse melanoma cells were investigated. This study suggested that ellagic acid had a strong inhibitory activity against tyrosinase and cell proliferation,which laid an experimental foundation for the development of new drugs and whitening products. The combined multispectral methods used in this research can be applied to the screening of other antityrosinase inhibitors, further promoting the development and utilization of tyrosinase inhibitors.
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Agaricales/enzimologia , Ácido Elágico/farmacologia , Melanoma/tratamento farmacológico , Animais , Proliferação de Células/efeitos dos fármacos , Ácido Elágico/química , Ligação de Hidrogênio/efeitos dos fármacos , CamundongosRESUMO
We studied the molluscicidal activity of Chimonanthus nitens extracts on Pomacea canaliculata (Ampullariidae). The degree of hepatopancreatic tissue damage, and its physiological and biochemical effects, was evaluated on individuals exposed to petroleum ether extracts (PEEEs). The PEEEs, ethyl acetate extract (EAEE) and water saturated n-butyl extract (SBEE) of C. nitens also had toxic effects on P. canaliculata but PEEE had the greatest molluscicidal activity. After exposure to PEEE for 24â¯h, the hepatopancreas of P. canaliculata had a large necrotic area. The levels of soluble sugar, soluble protein and albumin (Alb) in the hepatopancreas of P. canaliculata decreased with increasing PEEE concentration, while the activities of glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT) and acetylcholinesterase (AchE) increased with increasing PEEE concentration. A total of 29 compounds were identified from the PEEE of C. nitens by gas chromatography-mass spectrometry analysis. The main components were esters (48.13%), alcohols (18.43%) and the compound Chimonanthine (14.70%). The results of the molluscicidal assay, histological experiments and the physiological and biochemical experiments show that the PEEE of C. nitens could potentially be used for P. canaliculata management.
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Calycanthaceae/química , Flores/química , Extratos Vegetais/toxicidade , Caramujos/efeitos dos fármacos , Animais , Hepatopâncreas/efeitos dos fármacos , Hepatopâncreas/enzimologia , Hepatopâncreas/metabolismo , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de TransmissãoRESUMO
The inhibition of α-glucosidase and glycation is considered as an effective approach for the treatment of type 2 diabetes. In this study, multispectroscopic and molecular docking techniques were employed to investigate the inhibition of tannic acid on α-glucosidase and glycation. Kinetics analyses revealed that tannic acid had a significant inhibition on α-glucosidase (IC50â¯=â¯0.35⯱â¯0.02⯵M) in a reversible and mixed competitive manner. The results acquired from fluorescence quenching and ANS-binding fluorescence methods revealed that tannic acid could bind to α-glucosidase and reduce the hydrophobic area on the surface of the enzyme. In addition, synchronous fluorescence analysis showed that tannic acid decreased the hydrophobicity of α-glucosidase and changed the conformation of the enzyme. In vitro glycation assays showed that tannic acid had strong inhibitory effects on the formation of fructosamine, dicarbonyl compounds, and fluorescent AGEs. ANS-binding fluorescence analysis showed that tannic acid could bind to BSA and reduce the hydrophobicity of BSA in glycation. Moreover, the results of molecular docking showed the interaction between tannic acid and α-glucosidase was mainly driven by hydrogen bond, electrostatic, and hydrophobic interaction. And the interaction between tannic acid and BSA was mainly driven by hydrogen bond and hydrophobic interaction.
Assuntos
Inibidores de Glicosídeo Hidrolases/farmacologia , Taninos/farmacologia , alfa-Glucosidases/metabolismo , Inibidores de Glicosídeo Hidrolases/química , Glicosilação , Ligação de Hidrogênio , Modelos Moleculares , Estrutura Molecular , Conformação Proteica , Taninos/químicaRESUMO
Visible and near-infrared reflectance (VNIR) spectroscopy is considered to be a potential and efficient means for monitoring soil arsenic (As) contamination. While current studies mainly focus on the evaluation of models' performance when training and verification samples are collected from the same region, whether the model developed at a specific region can be transferred to other regions is still unclear. To answer this question, this study collected a total of 247 samples for training and verification from regions with different geographical conditions, which are Yuanping and Baoding in northern China, Chenzhou and Hengyang in southern China. Afterward, we proposed a transfer component analysis (TCA) based spectroscopic diagnosis model, which aims at adapting a model learned from one region to other regions. This model was compared with the traditional modeling method in terms of the prediction accuracy by four experiments. The results show that: (1) The traditional modeling method trained by specific regional samples has no transfer capability to different regions, since the coefficient of determination (R2) and the ratio of prediction to deviation (RPD) were 0.02 and 0.65 for the first pair of study areas, 0.01 and 1.01 for the second pair of study areas; (2) A transfer model with favorable predictability can be constructed with the aid of TCA spectral transformation and a small amount off-site samples (R2 and RPD were improved to 0.68 and 1.54 for the first pair of study areas, 0.64 and 1.66 for the second pair of study areas). Results suggest that it is promising to develop potential implementations of transferable spectroscopic diagnosis models for estimating soil As concentrations in large area with lower cost.
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A free-of-dust pollution extraction method combined-homogenate and ultrasonic cavitation system, namely, homogenate-combined ultrasonic cavitation synergistic extraction (HUCSE), was proposed for the efficient extraction of flavonoids from Cinnamomum camphora leaves. Response surface methodology of Box-Behnken design was employed to optimize the HUCSE process, and the optimum operation conditions attained with an extraction yield of 7.95 ± 0.27 mg/g were ethanol concentration 76%, homogenate/ultrasonic time 25 min, solvent-to-solid ratio 22 mL/g, and ultrasonic power 240 W. A second-order kinetic mathematical methodology was performed to depict the behaviors of HUCSE and heat reflux extraction method. The results suggested that the developed HUCSE is an efficient and green method for the extraction of C. camphora flavonoids or other plant natural products, where the obvious higher parameters of extraction capacity at saturation, second-order extraction rate constant, and original extraction rate were obtained when compared to the heat reflux method. The antioxidant activity assays in vitro showed that the C. camphora flavonoids possessed strong antioxidant activity and are promising to be applied as a natural alternative antioxidant.
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Extracts from the aerial parts of Solidago canadensis L. were evaluated for molluscicidal activity against Pomacea canaliculata Lam. using an immersion bioassay method. The petroleum ether fraction of the ethanolic extract (PEEE) from S. canadensis exhibited strong molluscicidal activity. The PEEE mode of action in the hepatopancreas tissue of P. canaliculata was tested at several concentrations. Biochemical parameters, namely, soluble sugar content, protein, malondialdehyde (MDA), acetylcholinesterase (AChE) activity, alanine aminotransferase (ALT), and aspartate transaminase (AST) were significantly decreased or increased after exposure to PEEE for 48 h (p<0.05). Histological assessment results showed that hepatopancreas tissue structure was destroyed by exposure to PEEE. Gas chromatography-mass spectrometry analysis (GC-MS) was used to identify 15 compounds that could contribute to the molluscicidal efficacy of the PEEE. Molluscicidal assay, biochemical tests and histological assessments suggest that the PEEE from S. canadensis has potential utility as a molluscicide.