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1.
Anticancer Res ; 33(4): 1585-9, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23564801

RESUMO

A case report of lethal distant myonecrosis with gas gangrene is presented. Blood cultures and tissue biopsies revealed Clostridium septicum. The 55-year-old female patient presented with recurrent ovarian cancer of transitional cell type, initially diagnosed as FIGO IIb in January 2011, with hepatic metastasis and invasion of the ceacal wall. She underwent several operations, including partial bowel and liver resection in September 2011. Second-line therapy with topotecan three weekly was started in October 2011 while the patient was still in the hospital. During this chemotherapy, the patient revealed symptoms of severe pain and erythema of the skin. Within hours she died of sceptic shock after a debridement. The diagnosis was gas gangrene due to Clostridium septicum. Because it is a rare and severe disease and the time slot in which therapeutic measures can be taken is narrow, we discuss clinical symptoms and therapeutic options.


Assuntos
Carcinoma de Células de Transição/complicações , Gangrena Gasosa/etiologia , Neoplasias Hepáticas/complicações , Doenças Musculares/etiologia , Recidiva Local de Neoplasia/diagnóstico , Neoplasias Ovarianas/complicações , Carcinoma de Células de Transição/microbiologia , Carcinoma de Células de Transição/patologia , Clostridium septicum/fisiologia , Desbridamento , Evolução Fatal , Feminino , Gangrena Gasosa/patologia , Humanos , Neoplasias Hepáticas/microbiologia , Neoplasias Hepáticas/secundário , Pessoa de Meia-Idade , Doenças Musculares/patologia , Neoplasias Ovarianas/microbiologia , Neoplasias Ovarianas/patologia
2.
Biochem Biophys Res Commun ; 367(1): 137-43, 2008 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-18157936

RESUMO

Mitochondrial aldehyde dehydrogenase (ALDH-2) reduces reactive oxygen species (ROS) formation related to toxic aldehydes; additionally, it provides a bioactivating pathway for nitroglycerin. Since acetaldehyde, nitroglycerin, and doxorubicin treatment provoke mitochondrial oxidative stress, we used ALDH-2(-/-) mice and purified recombinant human ALDH-2 to test the hypothesis that ALDH-2 has an indirect antioxidant function in mitochondria. Antioxidant capacity of purified ALDH-2 was comparable to equimolar doses of glutathione, cysteine, and dithiothreitol; mitochondrial oxidative stress was comparable in C57Bl6 and ALDH-2(-/-) mice after acute challenges with nitroglycerin or doxorubicin, whereas chronic acetaldehyde, nitroglycerin, and doxorubicin treatment dose-dependently increased mitochondrial ROS formation and impaired endothelial function to a greater extent in ALDH-2(-/-) mice. Maximal nitroglycerin dose applied in vivo lead to a "super-desensitized" nitroglycerin response in isolated ALDH-2(-/-) aortas, inaccessible in C57Bl6 mice. Our results suggest that ALDH-2 has an indirect antioxidative property independent of its thiol-moiety in disease states of cardiovascular oxidative stress.


Assuntos
Aldeído Desidrogenase/deficiência , Antioxidantes/farmacologia , Sistema Cardiovascular/efeitos dos fármacos , Mitocôndrias/enzimologia , Estresse Oxidativo/efeitos dos fármacos , Acetaldeído/farmacologia , Aldeído Desidrogenase/metabolismo , Aldeído-Desidrogenase Mitocondrial , Animais , Sistema Cardiovascular/metabolismo , Sistema Cardiovascular/patologia , Cisteína/farmacologia , Ditiotreitol/farmacologia , Relação Dose-Resposta a Droga , Doxorrubicina/farmacologia , Glutationa/farmacologia , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Modelos Biológicos , Nitroglicerina/farmacologia , Estresse Oxidativo/fisiologia , Espécies Reativas de Oxigênio/metabolismo
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