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1.
Prep Biochem Biotechnol ; 52(1): 30-37, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-33787455

RESUMO

The present study evaluated the influence of the variables polyethylene glycol (PEG) molar mass, pH, PEG concentration and sodium citrate concentration in the integrated production of the protease from Aspergillus tamarii Kita UCP1279 by extractive fermentation, obtaining as a response the partition coefficient (K), activity yield (Y) and concentration factor (CF). The enzyme preferably partitioned to the top phase and obtained in the system formed by variables MPEG = 400 g mol-1, CPEG = 20% (w w-1), and CCIT = 20% (w w-1) and pH 6, in this condition were obtained CF = 1.90 and Y = 79.90%. The protease showed stability at a temperature of 60 °C for 180 min, with optimum temperature 40 °C and pH 8.0. For the ions and inhibitors effects, the protease activity increased when exposed to Fe2+, Ca2+ and Zn2 + and inhibited by EDTA, being classified as metalloprotease. The kinetic parameters Km (35.63 mg mL-1) and Vmax (1.205 mg mL-1 min-1) were also estimated. Thus, the protease showed desirable characteristics that enable future industrial applications, especially, for beer industry.


Assuntos
Aspergillus/metabolismo , Ácido Cítrico/química , Proteínas Fúngicas/metabolismo , Peptídeo Hidrolases/metabolismo , Polietilenoglicóis/química , Estabilidade Enzimática , Fermentação , Proteínas Fúngicas/isolamento & purificação , Concentração de Íons de Hidrogênio , Microbiologia Industrial , Peptídeo Hidrolases/isolamento & purificação , Temperatura
2.
Prep Biochem Biotechnol ; 50(6): 619-626, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32013723

RESUMO

The protease from Aspergillus tamarii Kita UCP1279 extraction by aqueous two-phase PEG-Citrate (ATPS) systems, using a factorial design 24, was investigated. Then, the variables studied were polyethylene glycol (PEG) molar mass (MPEG), concentrations of PEG (CPEG) and citrate (CCIT), and pH. The responses analyzed were the partition coefficient (K), activity yield (Y) and purification factor (PF). The thermodynamic parameters of the ATPS partition were estimated as a function of temperature. ATPS was able to pre-purify the protease (PF = 1.6) and obtained 84% activity yield. The thermodynamic parameters ΔG°m (-10.89 kJ mol-1), ΔHm (-5.0 kJ mol-1) and partition ΔSm (19.74 J mol-1 K-1) showed that the preferential migration of almost all protein contaminants of the crude extract to the salt-rich phase, while the preferred protease was the PEG rich phase. The extracted enzyme presents optimum temperature and pH at range of 40-50 °C and 9.0-11.0, respectively. Moreover, the enzyme was identified as serine protease based on inhibition profile. ATPS showed the satisfactory performance as the first step for Aspergillus tamarii Kita UCP1279 protease pre-purification.


Assuntos
Aspergillus/enzimologia , Polietilenoglicóis/química , Serina Proteases/biossíntese , Serina Proteases/isolamento & purificação , Citrato de Sódio/química , Termodinâmica , Água/química , Concentração de Íons de Hidrogênio , Íons/farmacologia , Metais/farmacologia , Peso Molecular , Inibidores de Proteases/farmacologia , Transdução de Sinais/efeitos dos fármacos , Temperatura
3.
Int J Biol Macromol ; 118(Pt B): 1655-1666, 2018 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-30170368

RESUMO

Circular dichroism (CD) and fluorescence spectroscopy (FS) were used to monitor the pH-dependent conformational and structural stability changes induced by temperature and UV light on the protease from Aspergillus tamarii URM4634 at different pH values. The formation of photoproducts, such as N-formylkynurenine, dityrosine and kynurenine, were monitored with FS. The pH-dependent melting temperatures (Tm) were determined using CD and FS from 20 to 90 °C. Conformational changes were correlated with the pH-dependent biochemical activities. CD revealed that the protease is rich in α-helices. Thermal denaturation was irreversible at all pH range and displayed Tm values from 42.8 to 67.8 °C (CD) and from 38 to 60.3 °C (FS), which the highest Tm was observed at pH 6. The light and temperature induced to the formation of photoproducts was more intense at high pH value. Despite the biochemical data shows optimum pH 9, the highest stability was at pH 6, maintaining 100% of activity after 24 h. The acquired data permits to select the best physicochemical parameters to secure the optimal activity and stability when used in biotechnological applications. Furthermore, the conformal changes induced by temperature in the protein are directly correlated with its level of biochemical activity.


Assuntos
Aspergillus/enzimologia , Peptídeo Hidrolases/química , Peptídeo Hidrolases/metabolismo , Processos Fotoquímicos , Estabilidade Enzimática , Estrutura Secundária de Proteína
4.
Int J Biol Macromol ; 117: 1081-1088, 2018 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-29870814

RESUMO

An extracellular serine-protease from Aspergillus tamarii URM4634 was purified and characterized. The possibility of using Aspergillus tamarii URM4634 protease in detergent formulations and collagenolytic activity was investigated. The protease demonstrated excellent stability at pH range 7.0-11.0, the optimum being at pH 9.0. The enzyme was stable at 40 °C for 180 min, enhanced by Mg++ and Ca++, but inhibited by Zn++, and strongly inhibited by phenylmethylsulfonyl fluoride (PMSF), suggested as serine-protease. The azocasein substrate result showed Km = 0.434 mg/mL and Vmax = 7.739 mg/mL/min. SDS-PAGE and azocasein zymography showed that the purified alkaline protease (2983.8 U/mg) had a molecular mass of 49.3 kDa. The enzyme was purified by column chromatography using Sephadex A50 resin. The proteolytic activity was activated by SDS (sodium dodecyl sulfate), Tween-80, Tween 20 and Triton-100. This study demonstrated that A. tamarii URM4634 protease has potent, stable and compatible collagenolytic activity to the desired level in local laundry detergent brands compared with similar enzymes produced by solid-state fermentation. This protease can thus be chosen as an option in both the food industry to tenderization meat and the detergent industry to washing process.


Assuntos
Aspergillus/enzimologia , Colagenases/química , Colagenases/isolamento & purificação , Serina Proteases/química , Serina Proteases/isolamento & purificação , Cromatografia por Troca Iônica , Colagenases/metabolismo , Detergentes/farmacologia , Ativação Enzimática , Estabilidade Enzimática/efeitos dos fármacos , Espaço Extracelular/enzimologia , Fermentação , Concentração de Íons de Hidrogênio , Íons , Cinética , Metais , Peso Molecular , Proteólise , Serina Proteases/metabolismo , Temperatura
5.
Int J Biol Macromol ; 115: 1088-1093, 2018 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29723625

RESUMO

The kinetics and thermodynamics of Aspergillus aculeatus pectinase, either free or immobilized in alginate beads, were investigated. Pectinase immobilization ensured an enzyme immobilization yield of 59.71%. The irreversible denaturation of pectinase in both preparations was evaluated at temperatures ranging from 30 to 60 °C. When temperature was raised, the first-order thermal denaturation constant increased from 0.0011 to 0.0231 min-1 for the free enzyme and from 0.0017 to 0.0700 min-1 for the immobilized one, respectively. The results of residual activity tests enabled us to estimate, for denaturation of both free and immobilized pectinase, the activation energy (E⁎d = 85.1 and 101.6 kJ·mol-1), enthalpy (82.59 ≤ ΔH⁎d ≤ 82.34 kJ·mol-1 and 99.11 ≤ ΔH⁎d ≤ 98.86 kJ·mol-1), entropy (-63.26 ≤ ΔS⁎d ≤ -63.85 J·mol-1·K-1 and -5.50 ≤ ΔS⁎d ≤ -5.23 J·mol-1·K-1) and Gibbs free energy (101.8 ≤ ΔG⁎d ≤ 104.7 kJ·mol-1 and 100.6 ≤ ΔG⁎d ≤ 102.0 kJ·mol-1). The integral activity of a continuous system using the free and immobilized enzyme was also predicted, whose results indicated a satisfactory enzyme long-term thermostability in both preparations at temperatures commonly used to clarify juice. These results suggest that both free and immobilized pectinase from A. aculeatus may be profitably exploited in future food industrial applications, with special concern to the immobilized enzyme because of its reusability.


Assuntos
Alginatos/química , Aspergillus/enzimologia , Enzimas Imobilizadas/química , Enzimas Imobilizadas/metabolismo , Microesferas , Poligalacturonase/química , Poligalacturonase/metabolismo , Estabilidade Enzimática , Ácido Glucurônico/química , Ácidos Hexurônicos/química , Cinética , Termodinâmica
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