Effects of natural products-based dentifrices on acrylic resin for denture bases: A simulated period of 4 years.

PURPOSE: To evaluate the antimicrobial activity of dentifrices based on Malva sylvestris and propolis and the effect on the acrylic resin. METHODS: The inhibitory effect against Candida albicans, Enterococcus faecalis and Streptococcus mutans was determined. The specimens of resin were divided into groups: Control (brushing with distilled water); brushing with Colgate; brushing with Protex; brushing with Malvatricin, brushing with Proporalcare Dental Gel and brushing with Green Propolis Dental Gel. Brushing was carried out in a brushing machine. Roughness and color were assessed. The data were analyzed by ANOVA, followed by the Bonferroni post test (α= 0.05). RESULTS: The products evaluated showed antimicrobial activity against all microorganisms (P< 0.05), except Proporalcare Dental Gel, which was not effective against E. faecalis. Greater roughness (P< 0.05) was observed in the samples treated with Colgate. The samples from control and Protex groups showed the lowest color variation and the highest luminosity, with a difference in relation to the others (P< 0.05), which showed higher values, although clinically acceptable. CLINICAL SIGNIFICANCE: The dentifrices based on natural products can be indicated for cleaning prostheses, since they showed antimicrobial activity and did not cause perceptible color changes of the resin tested. The dentifrices did not produce adverse effects on the surface of the resin or did so less than the conventional dentifrice.

Effect of silver vanadate on the antibiofilm, adhesion and biocompatibility properties of denture adhesive.

Aim: To evaluate the biological and mechanical properties of an adhesive with nanostructured silver vanadate (AgVO3). Materials & methods: Specimens in poly(methyl methacrylate) (PMMA) were treated with Ultra Corega Cream (UCCA) denture adhesive with or without AgVO3. Biofilms of Candida albicans, Candida glabrata and Streptococcus mutans were grown and the viable cells counted. Fluorescence microscopy was used. The viability of the VERO cell and adhesive strength were evaluated. Results: All concentrations of AgVO3 reduced the biofilm formation and showed no cytotoxic effect. At 5 min and 24 h, UCCA with 5 and 10% AgVO3 showed better performance, respectively. Conclusion: AgVO3 promoted the antibiofilm activity of the adhesive, with a positive effect on the adhesive strength, and was biocompatible.

What is this summary about? Some people wear false teeth called dentures. They use a special glue to keep these false teeth in their mouths. It is important to clean dentures well and remove the glue every day. If the dentures get dirty, they can cause infections of the gums. Doctors and dentists can help, but sometimes medicines do not work well. This study checked to see whether adding a medicine that can kill bacteria into the glue could stop gum swelling and other illnesses, or make them better. What were the results? The glue containing the medicine killed microbes like fungi and bacteria. It also stuck things together well and was safe to use. What do the results mean? Using this special glue could help people with dentures to avoid illness.

Laser irradiation prevents root caries: Microhardness and scanning electron microscopy analysis.

Context: A promising option for the prevention of dental caries is the use of laser irradiation. Aims: Evaluate the effects of Er:YAG, Nd:YAG, and CO2 laser irradiation, associated or not to 2% sodium fluoride (2% NaF), on root caries prevention. Material and Methods: One hundred and four human root dentin samples were divided in eight groups (n = 13). A 9-mm2-area on each dentin sample was delimited and treated as follows: G1: no treatment (control); G2: 2% NaF; G3: Er:YAG; G4: 2% NaF + Er:YAG; G5: Nd:YAG; G6: 2% NaF + Nd:YAG; G7: CO2; G8: 2% NaF + CO2. When used, the 2% NaF was applied before irradiation for 4 min. The samples were subjected to a 2-week cariogenic challenge, consisted of daily immersion in de-remineralizing solutions for 6 h and 18 h, respectively. Knoop hardness (KHN) were evaluated (10 g and 20 s) at different depths from the dentin surface. The samples (n = 3) were prepared for scanning electron microscopy (SEM). Microhardness data were analysed by one-way analysis of variance (ANOVA) and Fisher's test (α = 5%). Results: The Er:YAG laser group (KHN = 41.30) promoted an increase in acid resistance of the dentin (P < 0.05) when compared to all groups. There was no synergism between laser irradiation and 2% NaF application. Morphological changes were observed after irradiation with all lasers; carbonization and cracks were also observed, except when Er:YAG were used. Conclusions: Er:YAG laser irradiation can safely increase the acid resistance of the dentin surface of the root, since it promoted a significant increase in surface hardness. The application of 2% NaF did not result in a synergistic effect.

Effect of conventional and rolled cigarette smoke on the colour of artificial teeth and effectiveness of hygiene protocols in removing pigmentation - An in vitro study.

Context: Artificial teeth are widely used in oral rehabilitation. Despite the benefits, they are more susceptible to colour changes, causing aesthetic problems. Aims: To evaluate the effect of conventional cigarette and straw smoke on the colour of artificial teeth and the effectiveness of hygiene protocols in removing pigmentation. Material and Methods: Acrylic resin incisors were divided into two groups (n = 50): Exposed to conventional cigarette and straw smoke. Regarding the effectiveness of hygiene protocols, the teeth were divided into ten subgroups with predetermined immersion times. The colour was measured with a colorimeter. The CIE values L* a* b* were recorded before and after exposure to smoke and after hygiene protocols. Statistical analysis used T-test of independent samples and two-way ANOVA with Bonferroni post-test (α = 0.05). Results: Conventional (16.16 ± 1.65) and straw (16.29 ± 1.95) cigarettes provided clinically unacceptable ΔE values, with no significant difference between them (P = 0.719). Conventional cigarettes promoted less luminosity (ΔL = -12.68 ± 1.28) (P < 0.001) and straw greater tendency to yellow (Δb = 11.00 ± 1.46) (P < 0.001). The hygiene protocols influenced the ΔE, ΔL, and Δb of the samples, depending on the type of smoke (P < 0.05). Conclusions: The conventional and rolled cigarette smoke promote an unacceptable colour change in artificial teeth. Hygiene protocols with the use of brushing, in isolation or in association with chemical solutions are more effective in removing pigmentation caused by both types of cigarettes compared to only the chemical solution.

Effect of saliva and beverages on the surface roughness and hardnessof acrylic resin modified with nanomaterial.

PURPOSE: To evaluate the surface roughness and hardness of thermopolymerized acrylic resin incorporated with nanostructured silver vanadate (AgVO3) subjected to saliva and beverages. METHODS: The 128 specimens (5×5×2 mm) were prepared in thermopolymerized acrylic resin, according to the AgVO3 concentrations (n=32): 0%, 2.5%, 5% and 10%. The roughness and hardness were analyzed before and after immersion in saliva, Coca-Cola, orange juice and red wine, for 12 and 24 days. 2-way ANOVA and Bonferroni test (α= 0.05) were performed. RESULTS: After 12 days, Coca-Cola caused the highest roughness increase in the 2.5% group. The 10% group with saliva presented a higher roughness increase (P= 0.009). The control presented a decrease in roughness when in beverages (P< 0.05). After 24 days, orange juice and Coca-Cola produced a higher decrease in roughness in the control group (P< 0.05). After 12 days, saliva and wine produced a higher decrease in hardness of the 2.5% group (P< 0.05). Coca-Cola produced a decrease in hardness and wine an increase in hardness in the group with 10% AgVO3 concentration (P< 0.05). After 24 days, the group with 2.5% presented the highest decrease in hardness (P< 0.05). The immersions produced decreased hardness in the acrylic resin. Initially, there was an increase in roughness, however, over time, it decreased. CLINICAL SIGNIFICANCE: The control of oral biofilm is fundamental for the maintenance of the patient's oral health; however the incorporation of antimicrobial nanomaterial into prosthetic materials frequently exposed to saliva and beverages in the oral cavity interfered with the physical-mechanical properties of the products tested.

Influence of Er,Cr:YSGG laser on dentin acid resistance after erosive challenge.

PURPOSE: To evaluate the influence of the Er,Cr:YSGG laser with or without the 5% fluoride varnish on the acid resistance of dentin after erosive challenge. METHODS: 36 incisors were selected and sectioned, obtaining 72 specimens of 4 mm × 4 mm and randomly divided into eight groups (n = 9). In G1: application of Er,Cr:YSGG (0.1W; 5Hz, air 55%); G2: laser (0.25W; 5Hz, air 55%); G3: fluoride varnish + laser (0.1W; 5Hz, air 55%); G4: fluoride varnish + laser (0.25W, 5Hz, air 55%); G5: fluoride varnish + laser (0.1W; 5Hz, without air); G6: fluoride varnish + laser (0.25W, 5Hz, without air); G7: fluoride varnish and G8: no treatment. When used, the laser was irradiated without water cooling, scanning mode during 10 seconds. The surface roughness data were subjected to ANOVA. For wear profile, we used Kruskal-Wallis test and Dunn post-hoc, all with α= 0.05. RESULTS: The results showed no statistically significant difference when comparing the groups as regards to the surface roughness (P> 0.05). Regarding the percentage of lost volume, the G5 and G6 groups presented the best results (G5 = 7.8% and G6 = 8.5%), with the lowest loss of dentin volume compared to other groups (P< 0.05). The G8 group (no treatment) had the highest lost volume (G8 = 39.1% followed by the G7 group (fluoride varnish), which had 25.9%. CLINICAL SIGNIFICANCE: The use of Er,Cr:YSGG laser and fluoride varnish can be an effective method to increase the acid resistance of dentin after erosive challenges, and limit problems related to hypersensitivity.

Development of an Impression Material with Antimicrobial Properties for Dental Application.

PURPOSE: To evaluate the antimicrobial activity and physico-mechanical properties of an irreversible hydrocolloid in which nanostructured silver vanadate decorated with silver nanoparticles (AgVO3 ) was added at various concentrations (0% - control, 2.5%, 5%, and 10% by weight). MATERIALS AND METHODS: The agar diffusion method (n = 10) was used to evaluate the inhibitory effect on the following species: Streptococcus mutans, Staphyloccocus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. The gelation time, flow capacity and plastic deformation were verified (n = 10). The data were analyzed using the Kruskal-Wallis test followed by the Dunn post-test, or via one-way ANOVA with multiple comparisons with a Bonferroni adjustment depending on the distribution (α = 0.05). RESULTS: All percentages of the nanomaterial were able to promote the antimicrobial activity of a hydrocolloid, with the formation of an inhibition zone (p < 0.05). In general, there was a dose-dependent effect on antimicrobial activity: higher concentrations of the nanomaterial promoted greater action except in the cases of P. aeruginosa (p < 0.001; F = 51.74) and S. aureus (p < 0.001), where the highest inhibition was for the 2.5% group. No difference was found in the gelation time when the control was compared with the groups with AgVO3 (p > 0.05). The difference was between the 5% and 10% groups (p = 0.007), and the latter promoted an increase in time. The flow capacity of the hydrocolloid with 5% of AgVO3 was significantly lower when compared with the control (p = 0.034). The AgVO3 influenced the plastic deformation (p < 0.001) in such a way that concentrations of 5% (p = 0.010) and 10% (p < 0.001) promoted an increase in this property when compared with the control. CONCLUSIONS: AgVO3 can be incorporated into an irreversible hydrocolloid as an antimicrobial agent without promoting adverse effects on physical-mechanical properties when used in concentrations of 2.5%.

Effect of casting atmosphere on the marginal deficiency and misfit of Ni-Cr alloys with and without beryllium.

STATEMENT OF PROBLEM: The marginal adaptation of prosthetic crowns is still a significant clinical problem. PURPOSE: The purpose of this in vitro study was to evaluate the marginal deficiency and misfit of Ni-Cr alloys with and without beryllium under different casting conditions. MATERIAL AND METHODS: Four casting conditions were selected: flame-torch, induction/argon, induction/vacuum, and induction/air; and 2 alloys were used, Ni-Cr-Be and Ni-Cr. For each group, 10 metal specimens were prepared. Silicone indirect impressions and analysis of the degree of rounding were used to evaluate the marginal deficiencies of metal copings, and a standardized device for the setting pressure associated with optical microscopy was used to analyze the marginal misfit. Results were evaluated with 2-way ANOVA (α=.05), followed by the Tukey honest significant difference post hoc test, and the Pearson correlation test (α=.05). RESULTS: Alloy (P<.001) and casting technique (P<.001) were shown to affect marginal deficiencies. The Ni-Cr cast using the torch technique showed the highest marginal deficiency, and the Ni-Cr-Be cast in a controlled argon atmosphere showed the lowest (P<.001). Alloy (P=.472) and casting techniques (P=.206) did not affect the marginal misfit, but significant differences were found in the interaction (P=.001); the lowest misfit was achieved using the Ni-Cr-Be, and the highest misfit occurred with the molten Ni-Cr, using the cast torch technique. No correlation was found between deficiency and marginal misfit (r=.04, P=.69). CONCLUSIONS: The interactions demonstrated that the alloy containing beryllium that was cast in an argon atmosphere led to reduced marginal deficiency. Improved marginal adaptation can be achieved for the same alloy by using the torch technique.

Evaluation of antibiofilm and mechanical properties of new nanocomposites based on acrylic resins and silver vanadate nanoparticles.

OBJECTIVE: The purpose of this study was evaluate, for the first time, the impact of incorporation of nanostructured silver vanadate (ß-AgVO3) in antibiofilm and mechanical properties of dental acrylic resins (poly(methyl methacrylate), PMMA). DESIGN: The ß-AgVO3 was synthesized and characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, and microanalysis (SEM/EDS). Resins specimens were prepared with 0-10% wt.% ß-AgVO3 and characterized by SEM, XRD and optical microscopy. The antibiofim activity of the samples against Candida albicans and Streptococcus mutans was investigated by XTT reduction test, colony-forming units (CFUs), and confocal laser scanning microscopy (CLSM). The flexural strength, hardness, and surface roughness of the samples containing ß-AgVO3 were compared with the pure PMMA matrix. RESULTS: The incorporation of 10% ß-AgVO3 significantly reduced the metabolic activity of C. albicans and S. mutans (p<0.05). There was a reduction in microbial load (CFU/mL) of microorganisms for the different concentrations used (p<0.05), which was confirmed by confocal microscopy. The addition of ß-AgVO3 did not change the mechanical properties of hardness and surface roughness of the resins (p>0.05). However, flexural strength decreased with the addition of amounts greater than 1% (p<0.05). CONCLUSIONS: ß-AgVO3 additions in dental acrylic resin may have an impact on inhibition of biofilm of main microorganisms associated with dental prostheses. However, the viability of clinical use should be evaluated in function of changed promoted in some mechanical properties.

In vitro study of the antibacterial properties and impact strength of dental acrylic resins modified with a nanomaterial.

STATEMENT OF PROBLEM: The accumulation of bacteria on the surface of dental prostheses can lead to systemic disease. PURPOSE: The purpose of this in vitro study was to evaluate the growth of Staphylococcus aureus and Pseudomonas aeruginosa on the surface of autopolymerizing (AP) and heat-polymerizing (HP) acrylic resins incorporated with nanostructured silver vanadate (ß-AgVO3) and its impact strength. MATERIAL AND METHODS: For each resin, 216 circular specimens (9 × 2 mm) were prepared for microbiologic analysis and 60 rectangular specimens (65 × 10 × 3.3 mm) for mechanical analysis, according to the percentage of ß-AgVO3: 0%, control group; 0.5%; 1%; 2.5%; 5%; and 10%. After a biofilm had formed, the metabolic activity of the bacteria was measured using the XTT reduction assay (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (n=8), and the number of viable cells was determined by counting colony forming units per milliliter (CFU/mL) (n=8). Confocal laser scanning microscopy (CLSM) was used to complement the analyses (n=2). The mechanical behavior was evaluated by impact strength assays (n=10). Data were analyzed by 2-way ANOVA, followed by the Tukey honestly significant difference (HSD) post hoc test (α=.05). RESULTS: The addition of 5% and 10% ß-AgVO3 significantly decreased the metabolic activity of P. aeruginosa for both resins (P<.05). The HP resin promoted a greater reduction in metabolic activity than the AP resin (P<.05). No difference was found in the metabolic activity of S. aureus according to the XTT (P>.05). The number of CFU/mL for S. aureus and P. aeruginosa decreased significantly when 5% and 10% ß-AgVO3 were added (P<.001). These concentrations significantly reduced the impact strength of the resins (P<.001) because the system was weakened by the presence of clusters of ß-AgVO3. CONCLUSION: The addition of ß-AgVO3 can provide acrylic resins with antibacterial activity but reduces their impact strength. More efficient addition methods should be investigated.