Effect of high-concentrate diets with calcium lignosulfonate and cottonseed processing method on quantitative traits and non-carcass components of feedlot cull ewes.

This study examined the effects of cottonseed processing form and the inclusion of calcium lignosulfonate in high-concentrate diets for feedlot cull ewes on carcass traits and non-carcass components. Thirty Santa Inês cull ewes with an average body weight of 44.2  ±  5.2 kg and an average age of 50 months were distributed into collective stalls in a completely randomized design. The treatments consisted of diets including whole cottonseed, crushed cottonseed, whole cottonseed treated with lignosulfonate (100 g/kg, as fed), crushed cottonseed treated with lignosulfonate (100 g/kg, as fed), and a control diet without cottonseed. The experimental diets did not influence ( P > 0.05 ) average daily weight gain (0.195 kg/day), slaughter weight (51.74 kg), or in vivo biometric and on-carcass measurements. There was no difference ( P > 0.05 ) in loin-eye area or subcutaneous fat thickness as evaluated in vivo by ultrasound. There was no diet effect on hot carcass weight and yield (24.8 kg and 47.8 %), cold carcass weight and yield (24.2 kg and 46.8 %), or chilling loss (2.1 %). Non-carcass components did not differ in response to the diets ( P > 0.05 ). Dietary inclusion of calcium lignosulfonate increases the proportions of udder and liver relative to empty body weight ( P < 0.05 ). Neither the cottonseed processing method nor the inclusion of calcium lignosulfonate in high-concentrate diets for cull ewes affects their performance, biometric or morphometric measurements, non-carcass components, or qualitative traits of their carcass.

In vitro and in silico studies of the larvicidal and anticholinesterase activities of berberine and piperine alkaloids on Rhipicephalus microplus.

Rhipicephalus microplus is responsible for high economic losses in livestock and its control has become difficult due to the establishment of tick populations resistant to commercial acaricides. This study aimed to evaluate the in vitro larvicidal effect of the alkaloids berberine and piperine, and also to investigate their inhibitory mechanisms against the acetylcholinesterase enzyme. The effects of the alkaloids on larvae were observed through the immersion test at the following concentrations: 1.5; 3; 6; 12; 16 and 24 mM. Berberine and piperine presented larvicidal activity greater than 95 %, not differing from 100 % for the positive fipronil control (p > 0.05). Of the two alkaloids, piperine had a lower effective concentration (EC), with an EC50 of 6.04 mM. The acetylcholinesterase enzyme used in the study was obtained from R. microplus larvae (RmAChE) and the anticholinesterase activity was determined spectrophotometrically. The highest anticholinesterase activity, measured as inhibition concentration (IC), was observed for berberine (IC50 = 88.13 µM), while piperine showed lower activity (IC50 > 200 µM). Docking studies in RmAChE, followed by 10 ns molecular dynamics simulation, suggest that berberine stabilizes the RmAChE at lower Root-Mean-Square Deviation (RMSD) than Apo protein. Few hydrogen-bond interactions between berberine and RmAChE residues were balanced by hydrophobic and π-type interactions. Berberine fills preferentially the peripheral anionic site (PAS), which correlates with its non-competitive mechanism. These results suggest that berberine and piperine alkaloids have an in vitro acaricidal action on R. microplus larvae, and the likely mechanism of action of berberine is related to RmAChE inhibition when accessing the PAS residues. These findings could help the study of new natural products that could inhibit RmAChE and aid in the development of new acaricides.

Impact of high-concentrate diets with cottonseed associated with calcium lignosulfonate on the metabolic, productive, and carcass characteristics of feedlot lambs.

This study aimed to evaluate the metabolic, productive, and carcass parameters of feedlot lambs fed high-concentrate diets with cottonseed associated with calcium lignosulfonate. Treatments consisted of diets including whole cottonseed, crushed cottonseed, whole cottonseed with lignosulfonate (100 g/kg), crushed cottonseed with lignosulfonate (100 g/kg), and a control diet without cottonseed. Thirty ½ Dorper ½ Santa Inês, non-castrated male lambs with an average live weight of 24.9 ± 3.6 kg and an average age of 3.5 months were evaluated in a completely randomized design. There was no effect (P > 0.05) on the intakes of dry matter, organic matter, crude protein, and non-fibrous carbohydrates. The crushing of cottonseed and association with lignosulfonate increased (P < 0.01) the intake and digestibility of ether extract. No effect was observed (P > 0.05) for the concentrations of urine and plasma urea N, which averaged 616.2 and 108.6 mg/dL, respectively. There was a change (P < 0.01) in nitrogen balance for digested N in g/day. There was no effect (P > 0.05) on the urinary concentration of purine derivatives, except for uric acid excretions (P < 0.05). Lignosulfonate associated with cottonseed provided an average daily gain of 0.293 kg/day, which was higher than the 0.226 kg/day obtained without lignosulfonate, but lower than control (0.302 kg/day), which also showed higher values of carcass yield. High-concentrate diets formulated without cottonseed improve lamb production performance. The use of high-concentrate diets with cottonseed associated with calcium lignosulfonate provides greater weight gains in lambs.