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Biochem J ; 476(24): 3769-3789, 2019 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-31803904

RESUMO

The atherosclerosis prone LDL receptor knockout mice (Ldlr-/-, C57BL/6J background) carry a deletion of the NADP(H)-transhydrogenase gene (Nnt) encoding the mitochondrial enzyme that catalyzes NADPH synthesis. Here we hypothesize that both increased NADPH consumption (due to increased steroidogenesis) and decreased NADPH generation (due to Nnt deficiency) in Ldlr-/- mice contribute to establish a macrophage oxidative stress and increase atherosclerosis development. Thus, we compared peritoneal macrophages and liver mitochondria from three C57BL/6J mice lines: Ldlr and Nnt double mutant, single Nnt mutant and wild-type. We found increased oxidants production in both mitochondria and macrophages according to a gradient: double mutant > single mutant > wild-type. We also observed a parallel up-regulation of mitochondrial biogenesis (PGC1a, TFAM and respiratory complexes levels) and inflammatory (iNOS, IL6 and IL1b) markers in single and double mutant macrophages. When exposed to modified LDL, the single and double mutant cells exhibited significant increases in lipid accumulation leading to foam cell formation, the hallmark of atherosclerosis. Nnt deficiency cells showed up-regulation of CD36 and down-regulation of ABCA1 transporters what may explain lipid accumulation in macrophages. Finally, Nnt wild-type bone marrow transplantation into LDLr-/- mice resulted in reduced diet-induced atherosclerosis. Therefore, Nnt plays a critical role in the maintenance of macrophage redox, inflammatory and cholesterol homeostasis, which is relevant for delaying the atherogenesis process.


Assuntos
Aterosclerose/metabolismo , Macrófagos Peritoneais/metabolismo , NADP/metabolismo , Estresse Oxidativo , Transportador 1 de Cassete de Ligação de ATP/genética , Transportador 1 de Cassete de Ligação de ATP/metabolismo , Animais , Aterosclerose/induzido quimicamente , Aterosclerose/genética , Biomarcadores , Antígenos CD36/metabolismo , Dieta Hiperlipídica , Regulação da Expressão Gênica , Genótipo , Glutationa/metabolismo , Inflamação , Masculino , Camundongos , Camundongos Knockout , Mitocôndrias/metabolismo , Mutação , NADP Trans-Hidrogenases , Receptores de LDL/genética , Superóxidos/metabolismo
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