RESUMO
The relationship between DNA content and nuclear morphology in large cell lymphomas (LCLs) was investigated on lymph node imprints. Mean maximum nuclear diameter (mean MND), nuclear shape and chromatin pattern were evaluated microscopically on May-Grünwald-Giemsa-stained slides. DNA content and nuclear area were measured on Feulgen-stained slides by image analysis. Twelve of the 24 cases were DNA diploid and 12 tetraploid. The DNA diploid cases were characterized by medium large (mean MND 11.2-13.7 microns), round nuclei with a fine chromatin pattern. The DNA tetraploid cases had significantly (P < .01) larger (mean MND 13.0-19.1 microns) nuclei and a higher frequency of coarse chromatin pattern, nuclear irregularities and multilobation. One of the researchers, unaware of the DNA index, could predict the ploidy level in 80% of cases on morphology. The linear coefficient of correlation between mean MND and mean nuclear area was 0.84. We also found that nuclear area increased as the cell moved through the cell cycle. DNA content, related to ploidy and position in the cell cycle, is an important explanation for the variable nuclear morphology in LCLs.
Assuntos
Núcleo Celular/ultraestrutura , DNA/análise , Linfoma Difuso de Grandes Células B/ultraestrutura , Cromatina/química , Diploide , Humanos , Linfonodos/química , Linfonodos/ultraestrutura , Linfoma Difuso de Grandes Células B/química , Ploidias , Coloração e RotulagemRESUMO
Becker-type muscular dystrophy (BMD) is reported in two brothers. In one of the patients, the molecular demonstration of an in-frame deletion of exons 45, 46 and 47 has confirmed the clinical and pathological diagnosis of BMD. The autopsy of the other patient revealed mild neuronal losses in the anterior horns at C8, lumbar and sacral levels of the spinal cord. Mild neuronal losses in the spinal cord may explain the mixed type of neurogenic-myogenic features in the skeletal muscles of adult BMD patients.
Assuntos
Distrofias Musculares/genética , Adulto , Deleção Cromossômica , Éxons/genética , Ligação Genética/genética , Humanos , Masculino , Microscopia Eletrônica , Músculos/patologia , Distrofias Musculares/patologia , Exame Neurológico , Linhagem , Aberrações dos Cromossomos Sexuais/genética , Medula Espinal/patologia , Cromossomo XRESUMO
A case of adenocarcinoma arising in a cystic duplication of the ileum of a 34 year old woman is described. Only one case of adenocarcinoma in ileal duplication has been recorded previously. The adenocarcinoma arose from gastric mucosa of a tubular duplication. In this case the adenocarcinoma arose from primitive type intestinal mucose in a cystic duplication.
Assuntos
Adenocarcinoma/cirurgia , Neoplasias do Íleo/cirurgia , Íleo/anormalidades , Adenocarcinoma/complicações , Adenocarcinoma/patologia , Adulto , Cistos/congênito , Feminino , Humanos , Neoplasias do Íleo/complicações , Neoplasias do Íleo/patologiaRESUMO
To investigate the possible role of type 1 allergy in OME, the immunoglobulin E (IgE) content was determined in 58 middle ear effusions and matched sera, obtained from children with otitis media with effusion (OME). The IgE levels were assayed for IgE by the Phadebas paper radio immunosorbent test (PRIST). Eight cases (14%) showed elevated serum IgE levels, such that an atopic disorder was very likely. However, in nearly all cases the effusion/serum ratio (E/S) was less than one, showing a higher IgE level in the serum than in the effusion. A modified PAP technique for determining surface IgE on mast cells is presented. Positive results were obtained on nasal mucosa specimens in atopic patients with OME but further research on middle ear mucosa in OME is needed. Our results fail to support the concept of type 1 allergy as a major causative factor in OME.