The role of genetic diversity and arbuscular mycorrhizal fungal diversity in population recovery of the semi-natural grassland plant species Succisa pratensis.

BACKGROUND: Ecosystem restoration is as a critical tool to counteract the decline of biodiversity and recover vital ecosystem services. Restoration efforts, however, often fall short of meeting their goals. Although functionally important levels of biodiversity can significantly contribute to the outcome of ecosystem restoration, they are often overlooked. One such important facet of biodiversity is within-species genetic diversity, which is fundamental to population fitness and adaptation to environmental change. Also the diversity of arbuscular mycorrhizal fungi (AMF), obligate root symbionts that regulate nutrient and carbon cycles, potentially plays a vital role in mediating ecosystem restoration outcome. In this study, we investigated the relative contribution of intraspecific population genetic diversity, AMF diversity, and their interaction, to population recovery of Succisa pratensis, a key species of nutrient poor semi natural grasslands. We genotyped 180 individuals from 12 populations of S. pratensis and characterized AMF composition in their roots, using microsatellite markers and next generation amplicon sequencing, respectively. We also investigated whether the genetic makeup of the host plant species can structure the composition of root-inhabiting AMF communities. RESULTS: Our analysis revealed that population allelic richness was strongly positively correlated to relative population growth, whereas AMF richness and its interaction with population genetic diversity did not significantly contribute. The variation partitioning analysis showed that, after accounting for soil and spatial variables, the plant genetic makeup explained a small but significant part of the unique variation in AMF communities. CONCLUSIONS: Our results confirm that population genetic diversity can contribute to population recovery, highlighting the importance of within-species genetic diversity for the success of restoration. We could not find evidence, however, that population recovery benefits from the presence of more diverse AMF communities. Our analysis also showed that the genetic makeup of the host plant structured root-inhabiting AMF communities, suggesting that the plant genetic makeup may be linked to genes that control symbiosis development.

Response of the arbuscular mycorrhizal fungi diversity and community in maize and soybean rhizosphere soil and roots to intercropping systems with different nitrogen application rates.

Maize (Zea mays L.)/soybean (Glycine max L.) intercropping has been widely practiced in China, because of its effectiveness in improving crop yield and nitrogen utilization efficiency. However, the responses of indigenous arbuscular mycorrhizal fungal (AMF) diversity and communities in rhizosphere soil and roots to intercropping systems with different nitrogen application rates remain unclear. In this study, a field experiment was conducted with split-plot design, and AMF communities in crop rhizosphere soil and roots in monoculture and intercropping systems treated with different levels of nitrogen fertilization were investigated using Illumina MiSeq sequencing. Nitrogen fertilization significantly decreased the AMF alpha-diversity in maize rhizosphere soil, and no significant differences were observed between monocultured and intercropped maize. The Shannon index of soybean rhizosphere soil was significantly higher in intercropping treatments than in monoculture treatments for the corresponding nitrogen levels. The AMF diversity in the roots of maize showed different trends to those in the soil. The dominant genera in the present study were Glomus_f_Glomeraceae, Paraglomus, and Gigaspora, which occupied 55.52%, 9.18%, and 8.20% of the rhizosphere soil and 65.35%, 5.32%, and 17.16% of the roots, respectively. Our study showed that the abundance of the dominant genus, Glomus_f_Glomeraceae in maize soil and roots significantly increased in intercropping treatments compared with monoculture treatments, and it also increased with the increase in nitrogen application levels. In soybean soil and roots, the abundance of Glomus_f_Glomeraceae decreased with the increase in nitrogen application levels. The results of the redundancy and correlations analyses indicated that the changes in the AMF diversity and community in intercropping areas were significantly associated with alterations of the soil total nitrogen and alkali-hydrolysable nitrogen due to the interactions between maize and soybeans in intercropping systems with different nitrogen fertilizer application rates.