From farm to pharma: Investigation of the therapeutic potential of the dietary plants Apium graveolens L., Coriandrum sativum, and Mentha longifolia, as AhR modulators for Immunotherapy.

Autoimmune diseases represent a complex array of conditions where the body's immune system mistakenly attacks its own tissues. These disorders, affecting millions worldwide, encompass a broad spectrum of conditions ranging from rheumatoid arthritis and multiple sclerosis to lupus and type 1 diabetes. The Aryl hydrocarbon receptor (AhR) translocator, expressed across immune and other cell types, plays crucial roles in immune disorders and inflammatory diseases. With a realm towards natural remedies in modern medicine for disease prevention, this study investigates the electronic properties and behaviors of bioactive compounds from dietary sources, including Apium graveolens L. (Celery), Coriandrum sativum seeds (Coriander), and Mentha longifolia, as AhR modulators. Through comprehensive analysis (HOMO-LUMO, ESP, LOL, and ELF), electron-rich and -poor regions, electron localization, and delocalization are identified, contrasting these compounds with the toxic AhR ligand, TCDD. Evaluation of Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) properties reveals favorable pharmacokinetics without blood-brain barrier penetration, indicating drug-like characteristics. Molecular docking demonstrates stronger interactions of dietary flavonoid ligands with AhR transcription compared to TCDD. Molecular dynamics simulations confirm the stability of complexes and the sustainability of interactions formed. This research underscores the potential of natural compounds as effective AhR modulators for therapeutic interventions in immune-related disorders.

Fusarium Wilt of Coriander: Root Cause Analysis and Varietal Tolerance Development.

Since 2012, growers of coriander, Coriandrum sativum L., in Israel have been suffering from summer wilting that can result in entire fields collapsing. The current study aimed to determine the cause of the phenomenon and find a genetic solution to the problem. The disease was reproduced in a growth chamber using naturally-infested soil from a commercial field. Wilt became apparent within two weeks, and after ten weeks, all plants died compared to plants in sterilized soil from the same source. Fusarium oxysporum was isolated from infected plants, and Koch's postulates were completed. Sequence analysis of the Elongation Factor (EF1α) encoding gene of the pathogen had a 99.54% match to F. oxysporum f. sp. coriandrii. Several coriander varieties were screened for resistance or tolerance to the disease. In four independent experiments, only the cultivar 'Smadi' showed high tolerance, while other genotypes were susceptible. In a trial in a naturally infested field, the cultivar 'Smadi' outperformed the commercial cultivar 'Blair'. 'Smadi' provides a cropping solution to many Israeli farmers, yet this winter cultivar bolts early in the summer. There is a further need to characterize the tolerance mechanism and inheritance for informed breeding of late-bolting Fusarium-resistant coriander.

Detailed Phytochemical Composition, Cyto-/Hepatotoxicity, and Antioxidant/Anti-Inflammatory Profile of Moroccan Spices: A Study on Coriander, Caraway, and Mystical Cumin.

Coriander, caraway, and mystical cumin are famous for their aromatic properties and widely used in Moroccan cuisine. The nutritional/phytochemical composition of their seeds (used for food flavoring and preservation) were compared. Their antioxidant, anti-inflammatory, cytotoxic and hepatotoxic effects were also explored. The fat content was similar among the samples (13%), with monounsaturated fatty acids being predominant. The coriander and mystical cumin seeds were extremely rich in C18:1n9c (81 and 85%, respectively) while, in the caraway, C18:1n12 (25%) was found together with C18:1n9c (32%). The caraway seeds also presented a higher proportion of C18:2n6c (34%) than the other seeds (13 and 8%, correspondingly). γ-Tocotrienol was the major vitamin E form in all the samples. The caraway seeds contained double the amount of protein (~18%) compared to the other seeds (~8%) but, qualitatively, the amino acid profiles among all seeds were similar. The seeds were also rich in dietary fiber (40-53%); however, differences were found in their fiber profiles. Caraway showed the highest antioxidant profile and anti-inflammatory activity and an LC-DAD-ESI/MSn analysis revealed great differences in the phenolic profiles of the samples. Cytotoxicity (NCI-H460, AGS, MCF-7, and CaCo2) and hepatotoxicity (RAW 264.7) were not observed. In sum, besides their flavoring/preservation properties, these seeds are also relevant source of bioactive compounds with health-promoting activities.

Coriandrum sativum L., essential oil as a promising source of bioactive compounds with GC/MS, antioxidant, antimicrobial activities: in vitro and in silico predictions.

Introduction: Coriandrum sativum L. essential oil (CS-EO) is being evaluated in vitro for its antioxidant and antimicrobial properties, and its volatile compounds are to be identified as part of this exploratory study. Methods: The processes underlying the in vitro biological properties were explained using in silico simulations, including drug-likeness prediction, molecular docking, and pharmacokinetics (absorption, distribution, metabolism, excretion, and toxicity-ADMET). Chemical screening of CS-EO was conducted using gas chromatography-mass spectrometry (GC-MS). Five in vitro complementary techniques were used to assess the antioxidant activity of CS-EO: reducing power (RP), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging activity, ß-Carotene bleaching test (BCBT), and phosphomolybdenum assay (TAC). Results: According to GC-MS analysis, linalool (59.04%), γ-Terpinene (13.02%), and α-Pinene (6.83%) are the main constituents of CS-EO. Based on the in vitro antioxidant assay results, CS-EO has been found to have a superior antioxidant profile. Its estimated scavenging rates for ABTS+ are 0.51 ± 0.04 mg/mL, BCBT is 9.02 ± 0.01 mg/mL, and CS-EO is 1.52 ± 0.14 mg/mL. C. sativum demonstrated 6.13 ± 0.00 µg/mL for reducing power and 213.44 ± 0.45 mg AAE/mL for total antioxidant activity. The in vitro antimicrobial activity of CS-EO was assessed against five strains, including two gram-positive bacteria, two gram-negative bacteria, and one fungal strain (Candida albicans). Significant antibacterial and antifungal activities against all strains were found using the disc-diffusion assay, with zones of inhibition larger than 15 mm. The microdilution test highlighted the lowest MIC and MBC values with gram-positive bacteria, ranging from 0.0612 to 0.125% v/v for MIC and 0.125% v/v for MBC. The fungal strain's MFC was 1.0% v/v and its MIC was measured at 0.5%. Based on the MBC/MIC and MFC/MIC ratios, CS-EO exhibits bactericidal and fungicidal activity. The ADMET study indicates that the primary CS-EO compounds are good candidates for the development of pharmaceutical drugs due to their favorable pharmacokinetic properties. Conclusion: These results point to a potential application of this plant as a natural remedy and offer empirical backing for its traditional uses. It is a promising environmentally friendly preservative that can be used extensively in the food and agricultural industries to prevent aflatoxin contamination and fungal growth in stored goods.

Green Synthesis of Silver Nanoparticles using Coriandrum sativum and Murraya koenigii Leaf Extract and its Thrombolytic Activity.

BACKGROUND: Plants have been used for ages in traditional medicine, and it is exciting to perceive how recent research has recognized the bioactive compounds liable for their beneficial effects. Green synthesis of metal nanoparticles is a hastily emergent research area in nanotechnology. This study describes the synthesis of silver nanoparticles (AgNPs) using Coriandrum sativum and Murraya koenigii leaf extract and its thrombolytic activity. OBJECTIVE: The aim of the study was to determine the clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles. METHODS: Leaves of Coriandrum sativum and Murraya koenigii were collected. Methanolic extraction of the plant sample was done through a Soxhlet extractor. The methanolic extract obtained from both the leaves was subjected to GC-MS analysis. The synthesized NPs from leaf extracts were monitored for analysis, where the typical X-ray diffraction pattern and its diffraction peaks were identified. 3D image of the NPs was analysed by Atomic Force Microscopy. The surface charge of nanoparticles was identified by Zeta potential. The Clot lysis activity of Coriandrum sativum and Murraya koenigii synthesized silver nanoparticles were analysed by the modified Holmstorm method. RESULTS: The thrombolytic property of the methanolic extract of plants Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 45.99% activity, and Murraya koenigii extract with 66.56% activity. The nanoparticles (Nps) from Coriandrum sativum showed clot lysis activity at 2.5 mg/mL with 58.29% activity, and NPs from Murraya koenigii with 54.04% activity. Coriandrum sativum in GC-MS exhibited 3 peaks, whereas Murraya koenigii extract showed five peaks with notable bioactive compounds. CONCLUSION: These NPs were further used for biomedical applications after being fixed by an organic encapsulation agent. The present research reveals the usefulness of Coriandrum sativum and Murraya koenigii for the environmentally friendly manufacture of silver nanoparticles.

Antimalarial efficacy test of the aqueous crude leaf extract of Coriandrum sativum Linn.: an in vivo multiple model experimental study in mice infected with Plasmodium berghei.

BACKGROUND: Malaria continues to wreak havoc on the well-being of the community. Resistant parasites are jeopardizing the treatment. This is a wake-up call for better medications. Folk plants are the key starting point for antimalarial drug discovery. After crushing and mixing the leaves of Coriandrum sativum with water, one cup of tea is drunk daily for a duration of three to five days as a remedy for malaria by local folks in Ethiopia. Additionally, in vitro experiments conducted on the plant leaf extract elsewhere have also demonstrated the plant's malaria parasite inhibitory effect. There has been no pharmacologic research to assert this endowment in animals, though. This experiment was aimed at evaluating the antimalarial efficacy of C. sativum in Plasmodium berghei infected mice. METHODS: The plant's leaf was extracted using maceration with distilled water. The extract was examined for potential acute toxicity. An evaluation of secondary phytoconstituents was done. Standard antimalarial screening models (prophylactic, chemosuppressive, curative tests) were utilized to assess the antiplasmodial effect. In each test, thirty mice were organized into groups of five. To the three categories, the test substance was given at doses of 100, 200 and 400 mg/kg/day before or after the commencement of P. berghei infection. Positive and negative control mice were provided Chloroquine and distilled water, respectively. Rectal temperature, parasitemia, body weight, survival time and packed cell volume were ultimately assessed. Analysis of the data was performed using Statistical Package for Social Sciences. RESULTS: No toxicity was manifested in mice. The extract demonstrated a significant inhibition of parasitemia (p < 0.05) in all the models. The inhibition of parasite load was highest with the upper dose in the suppressive test (82.74%) followed by the curative procedure (78.49%). Likewise, inhibition of hypothermia, weight loss hampering, improved survival and protection against hemolysis were elicited by the extract. CONCLUSIONS: The results of our experimental study revealed that the aqueous crude leaf extract of C. sativum exhibits significant antimalarial efficacy in multiple in vivo models involving mice infected with P. berghei. Given this promising therapeutic attribute, in depth investigation on the plant is recommended.

The Efficacy of the Herbal Formula of Bunium persicum and Coriandrum sativum on Clinical Symptoms of Patients with Functional Dyspepsia: A Double-Blind Randomized Controlled Trial.

INTRODUCTION: Functional dyspepsia (FD) is one of the most prevalent chronic disorders affecting up to 30% of the world population with considerable impairment of quality of life. This study evaluated the efficacy of the herbal preparation of Bunium persicum (Boiss.) B.Fedtsch. and Coriandrum sativum L. on symptom severity, symptom frequency, and quality of life of patients with FD. METHODS: This double-blind randomized controlled clinical trial, with parallel groups allocation ratio of 1:1, was conducted in a referral clinic of Afzalipour Hospital affiliated with Kerman University of Medical Sciences in Kerman, southeastern Iran. A total of 90 patients with FD (diagnosed based on the Rome IV criteria) were included in this study. The patients with complications during the intervention, pregnancy or lactation, a history of allergy to herbal drugs, use of other medication that affect the symptoms of FD during the study, and severe organic or psychiatric disorders were excluded. The participants received one capsule containing 500 mg of herbal preparation or placebo twice a day for 4 weeks and were subsequently followed up for 4 more weeks. Clinical outcomes were assessed via the Dyspepsia Severity Questionnaire, the Rome IV Diagnostic Questionnaire, and Nepean Dyspepsia Index Questionnaire. RESULTS: A total 40 participants in each group completed the follow-up period, and their data were analyzed statistically. All clinical outcomes showed significant improvements by herbal preparation compared to the placebo without serious side effects (p < 0.05). CONCLUSIONS: This herbal preparation can significantly improve the quality of life, and symptoms of FD compared to placebo.

Characterization of volatile profile from different coriander (Coriandrum sativum L.) varieties via HS-SPME/GC-MS combined with E-nose analyzed by chemometrics.

Headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) and electronic nose (E-nose) technologies were implemented to characterize the volatile profile of aerial part from 40 coriander varieties. A total of 207 volatile compounds were identified and quantified, including aldehydes, alcohols, terpenes, hydrocarbons, esters, ketones, acids, furans, phenols and others. E-nose results showed that W5S and W2W were representative sensors responding to coriander odor. Among all varieties, the number (21-30 species) and content (449.94-1050.55 µg/g) of aldehydes were the highest, and the most abundant analytes were (Z)-9-hexadecenal or (E)-2-tetratecenal, which accounted for approximately one-third of the total content. In addition, 37 components were determined the characteristic constituents with odor activity values (OAVs) ≥ 1, mainly presenting citrusy, fatty, soapy and floral smells. Hierarchical cluster analysis (HCA) and principal component analysis (PCA) could effectively distinguish different varieties. This study provided a crucial theoretical basis for flavor evaluation and quality improvement of coriander germplasm resources.

The protective effects of some herbs on mitigating HFD-induced obesity via enhancing biochemical indicators and fertility in female rats.

The potential of plant-based diets and drugs to prevent and control obesity has been attributed to the presence of several biologically active phytochemicals. The study aimed to assess herb consumption's impact on alleviating the risks and hazards associated with obesity induced by a high-fat diet (HFD) and the promotion of fertility. Eighty rats were allocated into four distinct groups. Group 1 (G1) was provided with a basal diet and acted as the control group. Group 2 (G2) was provided with an HFD. Group 3 (G3) was provided with HFD supplemented with chia seeds and Hibiscus sabdariffa L. The fourth group of subjects was provided with HFD supplemented with Foeniculum vulgare (fennel) and Coriandrum sativum L. (coriander). The feeding session was sustained for 10 weeks, and the biochemical parameters were evaluated. The administration of Foeniculum vulgare (fennel) and Coriandrum sativum L. (coriander) (G4) resulted in a more significant reduction in all biochemical parameters compared to G3, which received a diet consisting of chia seeds and Hibiscus sabdariffa L. Additionally, the average number of embryonic lobes and the average number of offspring after birth were found to be considerably more significant in the normal control group (G1) and group (G4) compared to the HFD group (G2) and group (G3) (P < 0.01). Group 4 (G4) was administered a diet enriched with Foeniculum vulgare (fennel) and Coriandrum sativum L. (coriander), which demonstrated superior outcomes in many biochemical indicators and the promotion of fertility in obese female rats.

Screening of antimicrobial and insecticidal properties of essential oils extracted from three Tunisian aromatic and medicinal plants.

The essential oils (EOs) of Coriandrum sativum, Carum carvi and Artemisia herba-alba have been screened for their insecticidal activity against Tribolium castaneum, Sitophilus oryzae and Lasioderma serricorne and for their antimicrobial activity against Gram (+), Gram (-) bacteria and against yeast strain. Artemisia herba-alba EO showed good insecticidal activity with 24-h against L. serricorne (LC50 = 29.7) and against T. castaneum (66.1 µg/mL) and antibacterial potentials against Staphyloccocus aureus (MIC = 0.125 mg/mL). The antimicrobial activities of C. carvi EO, rich in D-carvone (72.4%) and D-limonene (23.8%) were particularly remarkable, with LC50 of 27.9 µg/mL against L. serricorne. Similarly, coriander EO, with linalool (64.6%) as the major compound was selected for its antimicrobial activities (Candida albicans, MIC = 1 mg/mL). These findings demonstrated that the tested EOs could be used as a natural source of insecticidal and antimicrobial activity, which may have a variety of uses in the food and pharmaceutical sectors.

Antimicrobial activity of Geranyl acetate against cell wall synthesis proteins of P. aeruginosa and S. aureus using molecular docking and simulation.

Incidences of Methicillin-Resistant Staphylococcus aureus and Multi-Drug Resistant Pseudomonas aeruginosa causing skin and soft tissue infections are becoming more prevalent due to repeated mutations and changes in the environment. Coriandrum sativum, a well-known Indian herbal medicinal plant, is shown to have antioxidant, antibacterial, and anti-inflammatory activity. This comparative study focuses on the molecular docking (PyRx v0.9.8) of ligand binding domains of WbpE Aminotransferase involved in O-antigen assembly in Pseudomonas aeruginosa (3NU7) and Beta-Lactamase found in Staphylococcus aureus (1BLC) with selected phytocompounds of Coriandrum sativum along with a known binder and a clinical reference drug. This was followed by molecular dynamics simulation studies (GROMACS v2019.4) for the docked complexes (with Geranyl acetate) with the best binding affinities (-23.4304 kJ/mol with Beta-Lactamase and -28.4512 kJ/mol with WbpE Aminotransferase) and maximum hydrogen bonds. Molecular dynamics simulation studies for both the proteins demonstrated that the complex with Geranyl acetate showed stability comparable to the complex with reference drug observed via Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF) and H-bond analyses. Changes in the secondary structural elements indicated that Geranyl acetate could possibly cause improper functioning of WbpE Aminotransferase leading to disrupted cell wall formation. Further, MM/PBSA analyses showed significant binding affinity of Geranyl acetate with WbpE Aminotransferase and Beta-Lactamase. This study aims to provide rationale for further studies of Coriandrum sativum as an antimicrobial, and to contextualise the results in the current scenario of growing antimicrobial resistance. HIGHLIGHTSPhytoconstituents present in Coriandrum sativum show significant binding affinity to the proteins in Pseudomonas aeruginosa and Staphylococcus aureus.Geranyl acetate exhibited the highest binding affinity with WbpE Aminotransferase involved in O-antigen assembly in Pseudomonas aeruginosa (PDB ID:3NU7) and Beta-Lactamase found in Staphylococcus aureus (PDB ID: 1BLC)Molecular dynamics simulation analyses show that the phytoconstituent, Geranyl acetate has an effect similar to the clinical reference drug, thus exhibiting potential antibacterial activity.Communicated by Ramaswamy H. Sarma.

The contribution of Coriandrum sativum in enhancing Oreochromis niloticus rearing at sub-optimal temperatures: effect on growth, health status, survival rate, and resistance to Aeromons Veronii.

This study (60 days) was conducted to investigate the ability of diet enriched with Coriandrum sativum powder or its extract to protect Oreochromis niloticus health and survivability at suboptimal temperature (21 ℃). One hundred and twenty (33.14 ± 0.5 g) were divided into four groups; each group has three replicates.. The first control group fed on a basal diet. Second and third groups fed on diet enriched with 30 mg/kg coriander seed powder (CP) and coriander seed ethanolic extract (CE), respectively. The fourth group (OT) fed on diet enriched with 500 mg oxytetracycline/kg diet. The results revealed that CE exhibited a considerable improvement in hematological parameters, hepatic-renal functions, antioxidant status, and immunological markers as well as remarkably increased resistance against Aeromonas veronii. It could be concluded that feeding tilapia CE enriched diet at 30 mg/kg is a recommended strategy to enhance tilapia health and resistance to A. veronii infection reared at 21 ℃.

Fitorremediación con Brassicaceae y Apiaceae en suelos contaminados con metales pesados

Introducción: La cuenca media del río Bogotá viene sufriendo contaminación por metales pesados debido a los vertidos industriales. Esta fuente de agua ha sido utilizada para el riego de cultivos de ciclo corto, lo que ha provocado la contaminación por metales pesados de los suelos productivos. Objetivo: Evaluar un proceso de fitorremediación con las especies de Brócoli (Brassica oleracea var. italica Plenck) y Cilantro (Coriandrum sativum L.) en suelos contaminados con metales pesados en Mosquera, Colombia. Métodos: El muestreo se realizó con un diseño factorial al azar: a) proporciones de cilantro/brócoli (30 cilantro/70 brócoli, 50/50, 70 cilantro/30 brócoli y dos controles de tipo monocultivo de cada especie) y b) tiempo de fitorremediación (3, 6 y 9 meses), cada tratamiento con cuatro parcelas experimentales. El suelo y el tejido vegetal se analizó mediante espectrofotometría de absorción atómica para determinar la concentración de los metales pesados. Resultados: Las concentraciones de metales pesados en las muestras de tejido vegetal para brócoli y cilantro mostraron valores de Cd, Pb y Cr más altos que Hg y As, respecto al control. Las concentraciones de Cd, Pb y Cr presentaron valores significativamente más altos (P < 0.05) en suelos con cilantro y brócoli mezclados, respecto al sitio de control donde la concentración de Cd registró niveles significativamente más bajos que en los otros suelos. Las concentraciones de Cd, Pb y Cr fueron más altas en tejidos de cilantro y en suelos con cilantro en comparación con el control. Conclusiones: La fitorremediación por medio de las especies Brassica oleracea var. itálica Plenck y Coriandrum sativum L. en proporciones 70/30 o 30/70 a suelos con pH entre 5.5 y 6.0 permiten una buena recuperación de suelos con contenidos de metales pesados en términos de concentraciones altas a cortos plazos, iniciando la biorremediación a los tres meses y finalizando cargas de disponibilidad variable a los nueve meses.

Introduction: The middle basin of the Bogotá River has been suffering from heavy metal contamination due to industrial discharges. This water source has been used to irrigate short-cycle crops, which has resulted in heavy metal contamination of productive soils. Objective: To evaluate a phytoremediation process with the plant species Broccoli (Brassica oleracea var. Italica plenck) and Cilantro (Coriandrum sativum L.) in soils contaminated with heavy metals in Mosquera, Colombia. Methods: Sampling was performed with a randomized factorial design: a) cilantro/broccoli proportions (30 cilantro/70 broccoli, 50/50, 70 cilantro/30 broccoli and two monoculture type controls of each species) and b) phytoremediation time (3, 6 and 9 months), each treatment with four experimental plots. Soil and plant tissue were analyzed in the laboratory by atomic absorption spectrophotometry to determine the concentration of heavy metals. Results: The concentrations of heavy metals in plant tissue samples for broccoli and cilantro showed higher values of Cd, Pb and Cr than Hg and As, with respect to the control. The concentrations of Cd, Pb and Cr presented significantly higher values (P < 0.05) in soil where there was a mixed presence of cilantro and broccoli, with respect to the control site where the concentration of Cd registered significantly lower levels than in the other soils. Finally, Cd, Pb and Cr concentrations were higher in cilantro tissues and in soils with cilantro compared to the control. Conclusions: Phytoremediation by means of Brassica oleracea var. italica Plenck and Coriandrum sativum L. in 70/30 or 30/70 proportions in soils with pH between 5.5 and 6.0 allows a good recovery of soils with heavy metal contents in terms of high concentrations in short terms, starting bioremediation after three months and ending loads of variable availability after nine months.

Impact of Coriandrum sativum seeds extract on albino rats' testicular toxicity caused by carbendazim.

Background: A broad spectrum carbamate fungicide called carbendazim (Carb) is used to combat a number of different fungal diseases. One of the extensively utilized medicinal plants in oriental countries is Coriandrum sativum. Aim: In the current study, the impact of C. sativum seeds extract (CSE) on albino rats' testicular toxicity caused by carbendazim was investigated. Materials and methods: A total of 50 male albino rats were classified into 5 groups [Gp1, Control Gp; Gp2, Coriandrum Gp (CSE); Gp 3, carbendazim Gp (Carb); Gp 4, Co treated CSE with Carb (CSE + Carb); Gp 5, Post treated Carb with CSE (Carb + CSE)]. Results: Carb induced elevation in serum LH. FSH, testicular malondialdehyde (MDA), testicular nitric oxide (NO) markers and testicular injury and it reduced serum testosterone, testicular glutathione (GSH), testicular catalase and PCNA. Treatments of Carb with CSE (CSE + Carb and/or Carb + CSE) improved these parameters and reduced testicular toxicity with best results for Carb + CSE than CSE + Carb. Conclusions: The above findings revealed that; Carb induced testicular toxicity and it supported the hypothesis that the antioxidant characteristics of one or more of CSE constituents can reduce the testicular toxicity of Carb.

Safety and efficacy of a feed additive consisting of an essential oil obtained from the fruit of Coriandrum sativum L. (coriander oil) (FEFANA asbl).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of an essential oil obtained from the fruit of Coriandrum sativum L. (coriander oil), when used as a sensory additive in feed for all animal species. The EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the use of coriander oil is safe up to the maximum proposed use levels of 30 mg/kg complete feed for piglets, pigs for fattening, sows, veal calves (milk replacer), cattle for fattening, sheep, goats and horses, salmonids, dogs and ornamental fish. For the other species, the calculated safe concentrations in complete feed are: 14 mg/kg for chickens for fattening, 28 mg/kg for laying hens, 26 mg/kg for turkeys for fattening, 28 mg/kg for rabbits and 14 mg/kg for cats. These conclusions were extrapolated to physiologically related species. For any other species, the additive was considered safe at 14 mg/kg complete feed. The proposed conditions of use for veal calves (10 mg/kg) and ruminants, horses and dogs (5 mg/kg) were considered safe provided that the use in water for drinking alone or in combination with the use in feed should not exceed the daily amount that is considered safe when consumed via feed alone. No concerns for consumers and the environment were identified following the use of coriander oil up to the maximum proposed use level in feed. Coriander oil should be considered as irritant to skin and eyes and the respiratory tract and as a dermal and respiratory sensitiser. Since C. sativum and its preparations are recognised to flavour food and its function in feed would be essentially the same as that in food, no further demonstration of efficacy was considered necessary.

Application of Silicon, Zinc, and Zeolite Nanoparticles-A Tool to Enhance Drought Stress Tolerance in Coriander Plants for Better Growth Performance and Productivity.

Drought stress in arid regions is a serious factor affecting yield quantity and quality of economic crops. Under drought conditions, the application of nano-elements and nano-agents of water retention improved the water use efficiency, growth performance, and yield quantity of drought-stressed plants. For this objective, two field experiments were performed and organized as randomized complete block designs with six replications. The treatments included kaolin (5 t. ha-1) bentonite (12.5 t. ha-1), perlite (1.25 t.ha-1), N-zeolite (1.3 L.ha-1), N-silicon (2.5 L.ha-1), and N-zinc (2.5 L.ha-1). The current study showed that the application of silicon, zinc, and zeolite nanoparticles only positively influenced the morphological, physiological, and biochemical properties of the drought-stressed coriander plant. Exogenous application of N-silicon, N-zinc, and N-zeolite recorded the higher growth parameters of drought-stressed plants; namely, plant fresh weight, plant dry weight, leaf area, and root length than all the other treatments in both seasons. The improvement ratio, on average for both seasons, reached 17.93, 17.93, and 18.85% for plant fresh weight, 73.46, 73.46, and 75.81% for plant dry weight, 3.65, 3.65, and 3.87% for leaf area, and 17.46, 17.46, and 17.16% for root length of drought-stressed plants treated with N-silicon, N-zinc, and N-zeolite, respectively. For physiological responses, the application of N-zeolite, N-silicon, and N-zinc significantly increased leaf chlorophyll content, photosynthetic rate, water use efficiency, chlorophyll fluorescence, and photosystem II efficiency compared with the control in both seasons, respectively. Similar results were observed in antioxidant compounds, nutrient accumulation, and phytohormones. In contrast, those treatments markedly reduced the value of transpiration rate, nonphotochemical quenching, MDA, ABA, and CAT compared to control plants. Regarding the seed and oil yield, higher seed and oil yields were recorded in drought-stressed plants treated with N-zeolite followed by N-silicon and N-zinc than all the other treatments. Application of N-zeolite, N-silicon and N-zinc could be a promising approach to improve plant growth and productivity as well as to alleviate the adverse impacts of drought stress on coriander plants in arid and semi-arid areas.

Coriandrum sativum and Aloysia triphylla can Protect the Development of Cancer: an in Vivo Study Using Mouse Painting Assay.

AIM: The aim of this study is to examine the protective properties of Coriandrum sativum and Aloysia triphylla against the development of skin cancer. METHOD: The skin cancer balb/c mouse model was utilized in the study. Plant extracts were administered to animals using oral gavage. In addition, skin cancer was induced using 7,12-dimethylbenz(a) anthracene (DMBA). RESULTS: The study found that A. triphylla extract reduced both tumor incidence (P<0.01) and papilloma frequency (P<0.001) and delayed the onset of tumor development (P<0.001). The A. triphylla extract did not affect tumor size in animals. C. sativum leaf extract reduced the number of tumors per animal, the incidence of tumors, and the frequency of papilloma (P<0.05). In addition, it delayed (P<0.01) the onset of tumors. Treatment of animals with C. sativum seed extract reduced the frequency of papilloma (P<0.05) and delayed the onset of tumors (P<0.05). However, the examined plant extracts did not impact the size of tumors induced by DMBA (P>0.05). CONCLUSION: The findings of this study revealed that C. sativum and A. triphylla could protect against cancer development as indicated using the animal model of skin painting assay.

Direct Evidence for Using Coriandrum sativum var. microcarpum Essential Oil to Ameliorate Scopolamine-Induced Memory Impairment and Brain Oxidative Stress in the Zebrafish Model.

Essential oil from Coriandrum sativum has been demonstrated to provide various pharmacological properties, such as antioxidant, antimicrobial, antibacterial, antifungal, antidiabetic, anticonvulsive, anxiolytic-antidepressant, and anti-aging properties. This study investigated the mechanism of Coriandrum sativum var. microcarpum essential oil (CSEO, 25, 150, and 300 µL/L) and cognitive impairment and brain oxidative stress in a scopolamine (SCOP, 100 µM) zebrafish model (Danio rerio) of cognitive impairment. Spatial memory, response to novelty, and recognition memory were assessed using the Y-maze test and the novel object recognition test (NOR), while anxiety-like behavior was investigated using the novel tank diving test (NTT). The cholinergic system activity and brain oxidative stress were also evaluated. CSEO was administered to zebrafish once a day for 21 days, while SCOP and galantamine (GAL, 1 mg/L) were delivered 30 min before behavioral testing and euthanasia. Our data revealed that SCOP induced memory dysfunction and anxiety-like behavior, while CSEO improved memory performance, as evidenced by behavioral tasks. Moreover, CSEO attenuated SCOP-induced brain oxidative stress and decreased acetylcholinesterase (AChE) activity. The results demonstrated the potential use of the CSEO in providing beneficial effects by reducing memory deficits and brain oxidative stress involved in the genesis of a dementia state.

Investigation of phytotherapeutic potential of herbal mixtures and their effects on salbutamol induced cardiotoxicity and hyperlipidemia in rabbits.

BACKGROUND: Cardiovascular diseases (CVDs) are the major cause of deaths all over the world. The high level of blood cholesterol and oxidative stress are major risk factors for heart diseases. The phytotherapeutics have attracted attention as potential agents for preventing and treating oxidative stress associated diseases. The objective of present study was to evaluate the synergetic cardio-protective and antilipidemic potential of medicinal plants viz. Coriandrum sativum, Piper nigrum and Cactus grandiflorus. Cardio-protective and anti-lipidemic potential of herbal mixture was evaluated against salbutamol induced cardiotoxicity in rabbits. For this purpose, rabbits were divided into six groups as normal control, salbutamol control, curative and standard drug curative. RESULTS: Salbutamol significantly (p < 0.05) increased the level of serum cardiac biomarkers (ALT, CK-MB, AST and LDH) and lipids (LDL, triglycerides, cholesterol) in rabbits. The prior and post administration of herbal mixture significantly (p < 0.05) lowered the elevated level of serum cardiac biomarkers and lipids equal to normal control. Gross pathological examination revealed that heart of salbutamol control animals became hardened, congested and were enlarged than preventive and curative groups. The phytotherapeutic analysis of medicinal plants revealed the presence of phenols, tannins, alkaloids and steroids. CONCLUSION: The results showed that this herbal mixture has strong cardio-protective and anti-lipidemic potential.

Coriandrum sativum and Its Utility in Psychiatric Disorders.

The negative impact on worldwide social well-being by the increasing rate of psychiatric diseases has led to a continuous new drug search. Even though the current therapeutic options exert their activity on multiple neurological targets, these have various adverse effects, causing treatment abandonment. Recent research has shown that Coriandrum sativum offers a rich source of metabolites, mainly terpenes and flavonoids, as useful agents against central nervous system disorders, with remarkable in vitro and in vivo activities on models related to these pathologies. Furthermore, studies have revealed that some compounds exhibit a chemical interaction with γ-aminobutyric acid, 5-hydroxytryptamine, and N-methyl-D-aspartate receptors, which are key components in the pathophysiology associated with psychiatric and neurological diseases. The current clinical evaluations of standardized extracts of C. sativum are scarce; however, one or more of its compounds represents an area of opportunity to test the efficacy of the plant as an anxiolytic, antidepressant, antiepileptic, or sleep enhancer. For this, the aim of the review was based on the pharmacological activities offered by the compounds identified and isolated from coriander and the processes involved in achieving their effect. In addition, lines of technological research, like molecular docking and nanoparticles, are proposed for the future development of phytomedicines, based on the bioactive molecules of C. sativum, for the treatment of psychiatric and neurological disorders addressed in the present study.