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Fish Shellfish Immunol ; 66: 390-397, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28478255

RESUMO

Danio fishes, a small type animal with short sexual cycles, are model vertebrate species. To investigate the genic evolution of this genus, the transcriptomes from Danio choprae and Danio albolineatus were sequenced by Illumina HiSeq 4000 platform. A total of 128,427,304 sequence reads from two Danio fishes were generated by Next Generation Sequencing. The resulting in two assemblies contained 88,682 and 88,029 unigenes in the Danio choprae and Danio albolineatus. Analysis of the orthologs from the Danio choprae and Danio albolineatus provided consistent evidence for the accelerated genic evolution in the Danio fishes. Several genes referring to immune functions under positive selection were identified by branch site model analysis, such as REL, GTF2E1, STAT6, MPG in Danio choprae and CYP17A1, ADORA2A, MYCN in Danio albolineatus. Our data provide novel insights into the adaptation in Danio fishes and is useful for understanding the genetic basis of adaptation in zebrafish.


Assuntos
Cyprinidae/genética , Evolução Molecular , Proteínas de Peixes/genética , Transcriptoma , Adaptação Biológica , Animais , Sequenciamento de Nucleotídeos em Larga Escala/veterinária , Filogenia
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