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1.
Emerg Infect Dis ; 22(2): 289-91, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26811984

RESUMO

On March 20, 2015, a case of Ebola virus disease was identified in Liberia that most likely was transmitted through sexual contact. We assessed the efficiency of detecting Ebola virus in semen samples by molecular diagnostics and the stability of Ebola virus in ex vivo semen under simulated tropical conditions.


Assuntos
Ebolavirus/genética , Doença pelo Vírus Ebola/diagnóstico , Doença pelo Vírus Ebola/microbiologia , Sêmen/virologia , Adulto , Linhagem Celular , Células Cultivadas , Feminino , Doença pelo Vírus Ebola/transmissão , Humanos , Masculino
2.
Biosens Bioelectron ; 75: 254-72, 2016 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-26319169

RESUMO

Ebola outbreak-2014 (mainly Zaire strain related Ebola virus) has been declared most widely spread deadly persistent epidemic due to unavailability of rapid diagnostic, detection, and therapeutics. Ebola virus disease (EVD), a severe viral hemorrhagic fever syndrome caused by Ebola virus (EBOV) is transmitted by direct contact with the body fluids of infected person and objects contaminated with virus or infected animals. World Health Organization (WHO) has declared EVD epidemic as public health emergency of international concern with severe global economic burden. At fatal EBOV infection stage, patients usually die before the antibody response. Currently, rapid blood tests to diagnose EBOV infection include the antigen or antibodies capture using ELISA and RNA detection using RT/Q-PCR within 3-10 days after the onset of symptoms. Moreover, few nanotechnology-based colorimetric and paper-based immunoassay methods have been recently reported to detect Ebola virus. Unfortunately, these methods are limited to laboratory only. As state-of-the art (SoA) diagnostics time to confirm Ebola infection, varies from 6h to about 3 days, it causes delay in therapeutic approaches. Thus developing a cost-effective, rapid, sensitive, and selective sensor to detect EVD at point-of-care (POC) is certainly worth exploring to establish rapid diagnostics to decide therapeutics. This review highlights SoA of Ebola diagnostics and also a call to develop rapid, selective and sensitive POC detection of EBOV for global health care. We propose that adopting miniaturized electrochemical EBOV immunosensing can detect virus level at pM concentration within ∼40min compared to 3 days of ELISA test at nM levels.


Assuntos
Técnicas Biossensoriais , Ebolavirus/isolamento & purificação , Doença pelo Vírus Ebola/diagnóstico , Surtos de Doenças , Ebolavirus/patogenicidade , Doença pelo Vírus Ebola/virologia , Humanos , Sistemas Automatizados de Assistência Junto ao Leito
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