RESUMO
In forensic practice, medicolegal physicians are often tasked with estimating age using dental evidence. This calls for an uncomplicated, reliable, and reproducible method for dental age estimation, enabling physicians to proceed without specific odontological expertise. Among various dental methods, third molar eruption analyses are less complicated and easier to perform. In our study, we explored the effectiveness of Gambier et al.'s scoring system, which examines the eruption of all third molars. We retrospectively analysed 1032 orthopantomograms (528 males and 504 females) of individuals aged between 15 and 24 years. The mean chronological age increased with the progression of stages (1 to 3) and phases (A to D) of the third molar eruption for both sexes. In terms of stages, none showed significant discrimination between minors (<18 years) and adults (>18 years), especially for males. However, Gambier's phase D displayed a relatively high likelihood of being 18 years or older, with an overall 85.9 % of males and 95.7 % of females having all third molars in stage 3 being 18 years or older. While the tested method could be helpful in indicating the completion of the 18th year of life, caution is advised (due to a high percentage of false positives), and it should be used alongside other age assessment methods by experts.
Assuntos
Determinação da Idade pelos Dentes , Dente Serotino , Radiografia Panorâmica , Humanos , Dente Serotino/diagnóstico por imagem , Determinação da Idade pelos Dentes/métodos , Adolescente , Masculino , Feminino , Adulto Jovem , Índia , Estudos Retrospectivos , Odontologia Legal/métodos , Adulto , Erupção DentáriaRESUMO
BACKGROUND: Malondialdehyde (MDA) is one of a dominat marker in oxidative stress condition, and when inflammation occurred tumor necrosis factor- α (TNF-α) played a significant influence in the propagation this process. Purified gambier (Uncaria gambier Roxb.) contained 90% catechin which is proven to have antioxidant activity and may prevent unwanted inflammatory responses during diabetic state. OBJECTIVE: The objective of this research was to assess how purified gambier affected plasma MDA and TNF- α levels in alloxan-induced diabetic rats. MATERIAL AND METHODS: In this study, 35 rats were used. Alloxan 120 mg/kg BW intraperitoneal injection was administered to induce diabetes conditions in rats. All animals were divided into 5 groups, diabetic control group treated with vehicle, positive control group treated with glibenclamide dose 0.45 mg/kg BW), and treatment groups treated with purified gambier dose of 2.5; 5 and 10 mg/kg BW. All animals were treated respectively for 14 days. Plasma MDA and TNF- α levels were measured on day 3, and 14. RESULTS: Two-way ANOVA was applied to analyze all of the data, these findings suggested that purified gambier has antioxidant-related anti-inflammation actions. possesses blood sugar-lowering activity (p<0.05). The plasma MDA and TNF- α level of treatment group were significantly reduced (p<0.05) compared to diabetes control group. CONCLUSION: These results depicted that at doses of 2.5-10 mg/kg BW, purified gambier has antioxidant-associated anti-inflammation effects when given for 14 days on diabetic rat model by reducing plasma levels MDA and TNF-α.
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Banana ripeness is generally determined based on physical attributes, such as skin color; however, it is considered subjective because it depends on individual factors and lighting conditions. In addition, improper handling can cause mechanical damage to the fruit. Intelligent packaging in the form of indicator film incorporated with anthocyanins from red dragon fruit peel has been applied for shrimp freshness detection; however, this film has low color stability during storage, necessitating the addition of gambier catechins as a co-pigment to increase anthocyanin stability. Nevertheless, the characteristics of films that contain gambier catechins and their applications to bananas have not been studied yet; therefore, this study aims to develop and characterize indicator films that were incorporated with red dragon fruit peel anthocyanins and gambier catechins to detect banana ripeness. In this study, the indicator films were made via solvent casting. The films were characterized for their structural, mechanical, and physicochemical properties, and then applied to banana packaging. The results show that the film incorporated with anthocyanins and catechins in a ratio of 1:40 (w/w) resulted in better color stability, mechanical properties, light and water vapor barrier ability, and antioxidant activity. The application of the indicator films to banana packaging resulted in a change in color on the third day of storage. It can be concluded that these films could potentially be used as an indicator to monitor banana ripeness.
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Background: Proteinuria is a significant clinical manifestation that causes edema in several diseases, including Nephrotic Syndrome (NS). Untreated proteinuria is strongly linked to the progression of kidney failure. One of the adjuvant therapies could be used to reduce proteinuria such as Angiotensin Receptor Blocker (ARB) including Losartan®. Gambier is a traditional medicinal plant widely known for its antioxidant effects. Catechin, a compound contained in Gambier Extract (GE), has been used to reduce microalbuminuria in diabetics. However, its application in NS has not been widely studied. Objective: This study compared the effects of GE and ARB in reducing proteinuria and increasing antioxidant activity levels, as well as reported histopathological findings in the nephrotic Wistar rat model. Methods: An experimental design study with a control group and a posttest was conducted. The experimental animals were divided into four groups: the control group (K1), the group with puromycin aminonucleoside (PAN) injection (K2), the group with PAN injection + GE (K3), and the group with PAN injection + Losartan® (K4). The standard GE used was Sarie Uncariae® by Toyo Brothers, PT while the ARB (Losartan®) was obtained from Novell, PT. Protein urine, the activity level of total superoxide dismutase (T-SOD), and malondialdehyde (MDA) were assessed using the colorimetric method. Renal histopathology was assessed based on Rollerman's criteria. Results: Gambier extract significantly reduced proteinuria, as depicted by a decrease in protein/volume urine (p = 0.009), increased antioxidant activity, as illustrated by an elevation in T-SOD activity levels (p = 0.007), and tended to decrease MDA levels compared to Losartan®. Based on histopathological findings, GE tended to reduce the percentage of kidney damage in rats induced by puromycin. Conclusion: Gambier extract has been shown a higher antioxidant effect by increasing T-SOD activity levels, reducing proteinuria and also exhibiting a tendency to diminish kidney damage.
Assuntos
Antioxidantes , Unha-de-Gato , Síndrome Nefrótica , Extratos Vegetais , Masculino , Ratos , Animais , Antioxidantes/farmacologia , Antioxidantes/uso terapêutico , Losartan/farmacologia , Losartan/uso terapêutico , Ratos Wistar , Antagonistas de Receptores de Angiotensina/efeitos adversos , Inibidores da Enzima Conversora de Angiotensina/efeitos adversos , Proteinúria/tratamento farmacológico , Superóxido Dismutase/efeitos adversos , Superóxido Dismutase/metabolismoRESUMO
Ciguatera poisoning (CP) cases linked to the consumption of deep-water fish occurred in 2003 in the Gambier Islands (French Polynesia). In 2004, on the request of two local fishermen, the presence of ciguatoxins (CTXs) was examined in part of their fish catches, i.e., 22 specimens representing five deep-water fish species. Using the radioactive receptor binding assay (rRBA) and mouse bioassay (MBA), significant CTX levels were detected in seven deep-water specimens in Lutjanidae, Serranidae, and Bramidae families. Following additional purification steps on the remaining liposoluble fractions for 13 of these samples (kept at -20 °C), these latter were reanalyzed in 2018 with improved protocols of the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography tandem mass spectrometry (LC-MS/MS). Using the CBA-N2a, the highest CTX-like content found in a specimen of Eumegistus illustris (Bramidae) was 2.94 ± 0.27 µg CTX1B eq. kg-1. Its toxin profile consisted of 52-epi-54-deoxyCTX1B, CTX1B, and 54-deoxyCTX1B, as assessed by LC-MS/MS. This is the first study demonstrating that deep-water fish are potential ciguatera vectors and highlighting the importance of a systematic monitoring of CTXs in all exploited fish species, especially in ciguatera hotspots, including deep-water fish, which constitute a significant portion of the commercial deep-sea fisheries in many Asian-Pacific countries.
Assuntos
Aquicultura , Ciguatera/prevenção & controle , Peixes , Animais , Organismos Aquáticos , Humanos , Camundongos , PolinésiaRESUMO
The inventories of 137Cs (503⯱â¯34 Bq.m-2), 241Am (11.3⯱â¯1.2 Bq.m-2), 241Pu (33.7⯱â¯3.4 Bq.m-2), 238Pu (6.82⯱â¯0.87 Bq.m-2) and 239+240Pu (113.0⯱â¯5.9 Bq.m-2), sum of 239Pu (100⯱â¯11 Bq.m-2) and 240Pu (14.5⯱â¯1.7 Bq.m-2), in the Gambier archipelago (23°S) of the French Polynesia, are well higher the global fallout at this latitude, in unequal proportions for the different radionuclides. (240Pu/239Pu)AR (AR: atomic ratio) of 0.0394⯱â¯0.0062, and (241Pu/239Pu)AR of (2.03⯱â¯0.39)10-4, confirm that the overwhelmingly dominant source of these radionuclides comes from local fallout during the 1970s of the French atmospheric tests of Moruroa and Fangataufa located nearly 400â¯km from Gambier. The signatures of the local fallout were deduced from the excess of its inventory in 137Cs and from the mixing lines established from the signatures of the global fallout, some of the test sites and the isotopic ratios measured in Gambier. Signatures obtained are 2.0⯱â¯0.4 for 137Cs/239+240Pu, 0.045⯱â¯0.008 for 238Pu/239+240Pu, 0.031⯱â¯0.009 for 241Am/239+240Pu, 0.092⯱â¯0.027 for 241Pu/239+240Pu, 0.0163⯱â¯0.0049 for (240Pu/239Pu)AR, (0.78⯱â¯0.23)10-4 for (241Pu/239Pu)AR. The concordance of the mixing lines of the [(240Pu/239Pu)AR, (241Pu/239Pu)AR] and the linear regression of these ratios measured in the stratosphere (40°S) during the 1970s, indicates that the signatures of the close-in deposition are also those of the stratospheric injections of the French tests. The signatures of stratospheric fallout in the Southern Hemisphere were evaluated by considering that the fission energy of these injections represents 11% and that of the Northern Hemisphere represents 89% and that the isotopic ratios of stratospheric injections remained the same over the period 1970-1974. The activity ratios deducted are 21.9⯱â¯0.1 in 137Cs/239+240Pu, 0.11⯱â¯0.05 in 238Pu/239+240Pu, 1.03⯱â¯0.12 in 241Pu/239+240Pu and 0.35⯱â¯0.04 in 241Am/239+240Pu. The associated atom ratios are 0.157⯱â¯0.011 for (240Pu/239Pu)AR and (8.33⯱â¯0.48)10-4 for (241Pu/239Pu)AR. These signatures appear to be consistent with the results of the inventories at Hiva Oa, located more than 1,000â¯km north of both French test sites, and with those found in the Australian continent, in regions not impacted by UK-test debris. The proportions of close-in tropospheric fallout from the French tests are about 90% in Gambier. They represent a proportion in the inventories of 40% for the 137Cs, 60% for 241Am and in the range between 80 and 90% for Pu isotopes.
Assuntos
Plutônio , Monitoramento de Radiação , Cinza Radioativa , Austrália , Radioisótopos de Césio , Ilhas , Plutônio/análise , Polinésia , Cinza Radioativa/análiseRESUMO
Monosodium glutamate (MSG) is often added in foods to enhance the flavor. It has adverse effect to body organs. Natural remedies, such as: gambier have been known for generations used to improve health. Substances contained in gambier, i.e.: catechins are believed to reduce the occurrence of hepatotoxicity. The study aims to analyze the effects of gambier in reducing the hepatotoxicity of MSG. This study with a posttest only control group design. Mice amount 25 (5 groups, n = 5/group). All interventions are given orally for 4 weeks. At the end of the study, it carried out euthanasia taken the liver of mice to made histopathology preparations then examine by light microscope, ×400, ×5 view field. Liver damage was found in each group with various levels of histological appearance:(I) 2 normal, 2 minimal, 1 moderate and none severe; (II) none normal, 1 minimal, 3 moderate and 1 severe; (III) 1 normal, 4 minimal, none moderate and severe;, (IV) 2 normal, 3 minimal, none moderate and severe; and (V) 4 normal, 1 minimal, none moderate, and severe. The data were analyzed using Kruskal-Wallis test. The level of liver damage among all groups was significantly different (p = 0.017). The same on the Dunn test also showed the level of liver damage in Group-II to compare with other groups (p < 0.05). The present study proves that Gambier (Uncaria gambier Roxb) can reduce occurrence of hepatotoxicity caused by MSG.
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The present study was intended to prepare and optimize the mucoadhesive buccal patch of gambier leaf extract using chitosan (CH) and tapioca starch (TS) composite as the polymer complexes. The patch formulation was designed based on 22 factorial design in order to optimize the composition of CH and TS. The physical and chemical characteristics of the prepared patches, including mass and thickness uniformity, folding endurance, surface pH, swelling index, percent of elongation, and mucoadhesive time were successfully evaluated. Based on statistical analysis, the optimum concentration of CH and TS was 900 mg and 300 mg, respectively, with desirability percent of 0.968. The characterization of the optimum patch showed that the variability coefficient of the mass and thickness uniformity was 0.4805 ± 0.1887% and 0.9716 ± 1.2026%, surface pH of the patch was 6, folding endurance >300 times, elongation percent was 53.333 ± 0.1082%, and mucoadhesive time was 320 ± 1.1547 min. The catechin content, as the active agent of the gambier leaf extract, was 92.1667 ± 0.3626%, and the FT-IR characterization indicated that there are no chemical interactions between each patch component.
Assuntos
Catequina/química , Quitosana/química , Gengivite/terapia , Manihot/química , Amido/química , Uncaria/química , Concentração de Íons de Hidrogênio , Extratos Vegetais/química , Folhas de Planta/química , Polímeros/química , Adesivo TransdérmicoRESUMO
A global transcriptional regulator, MgrA, was previously identified as a key determinant of virulence in Staphylococcus aureus. An 80% EtOH extract of Uncaria gambier was found to attenuate the virulence of S. aureus via its effects on MgrA. Using bioassay-guided fractionation, a polyphenolic polymer, uncariitannin, was found to be the main bioactive constituent of the extract, and its structure was characterized using spectral and chemical analysis. The molecular weight and polydispersity of uncariitannin were determined by gel permeation chromatography-refractive index-light scattering analysis. An electrophoretic mobility shift assay showed that uncariitannin could effectively inhibit the interaction of MgrA with DNA in a dose-dependent manner. Treatment with uncariitannin could decrease the mRNA and protein levels of Hla in both the S. aureus Newman and USA300 LAC strains. Further analysis of Hla expression levels in the Newman ΔmgrA and Newman ΔmgrA/pYJ335-mgrA strains indicated that uncariitannin altered Hla expression primarily in an MgrA-dependent manner. A mouse model of infection indicated that uncariitannin could attenuate MRSA virulence. In conclusion, uncariitannin may be a potential candidate for further development as an antivirulence agent for the treatment of S. aureus infection.
Assuntos
Antibacterianos , Polímeros , Polifenóis , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus/efeitos dos fármacos , Uncaria , Virulência/efeitos dos fármacos , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , Feminino , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Rim/efeitos dos fármacos , Rim/patologia , Fígado/efeitos dos fármacos , Fígado/patologia , Camundongos Endogâmicos BALB C , Miocárdio/patologia , Polímeros/farmacologia , Polímeros/uso terapêutico , Polifenóis/farmacologia , Polifenóis/uso terapêutico , Baço/efeitos dos fármacos , Baço/patologia , Infecções Estafilocócicas/patologia , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo , Staphylococcus aureus/patogenicidadeRESUMO
Rapa Nui, commonly known as Easter Island (Chile), is one of the most isolated tropical islands of the Pacific Ocean. The island location of Rapa Nui makes it the easternmost point of the geographic ranges for many western Pacific fish species that are restricted to the subtropical islands south of 20°S latitude. The blenniid fish species Cirripectesalboapicalis has been thought to have one of the most extensive geographic distribution ranges among these southern subtropical fish species, extending from the southern Great Barrier Reef to Rapa Nui. A phylogenetic analysis was conducted to determine the taxonomic status of the species. The results provide genetic evidence that suggests that this formerly South Pacific-wide species comprises at least three cryptic species with allopatric geographic distributions. The analyses reveal the geographic distributions of these clades and their genetic relationships with each other, and with other species within the genus Cirripectes. The processes that culminated in the current geographic distribution of this species complex and the zoogeographic implications of this finding for the South Pacific region are discussed.