Floral Trait Preferences of Three Common wild Bee Species.

The interaction between bees and flowering plants is mediated by floral cues that enable bees to find foraging plants. We tested floral cue preferences among three common wild bee species: Lasioglossum villosulum, Osmia bicornis, and Bombus terrestris. Preferences are well studied in eusocial bees but almost unknown in solitary or non-eusocial generalist bee species. Using standardized artificial flowers altered in single cues, we tested preferences for color hue, achromatic contrast, scent complexity, corolla size, and flower depth. We found common attractive cues among all tested bees. Intensively colored flowers and large floral displays were highly attractive. No preferences were observed in scent complexity experiments, and the number of volatiles did not influence the behavior of bees. Differing preferences were found for color hue. The specific behaviors were probably influenced by foraging experience and depended on the flower choice preferences of the tested bee species. In experiments testing different flower depths of reward presentation, the bees chose flat flowers that afforded low energy costs. The results reveal that generalist wild bee species other than well-studied honeybees and bumblebees show strong preferences for distinct floral cues to find potential host plants. The diverse preferences of wild bees ensure the pollination of various flowering plants.

Location and Creation of Nest Sites for Ground-Nesting Bees in Apple Orchards.

Wild ground-nesting bees are key pollinators of apple (Malus domestica). We explored, (1) where they choose to nest, (2) what influences site selection and (3) species richness in orchards. Twenty-three orchards were studied over three years; twelve were treated with additional herbicide to increase bare ground with the remainder as untreated controls. Vegetation cover, soil type, soil compaction, nest number and location, and species were recorded. Fourteen species of ground-nesting solitary/eusocial bee were identified. Most nests were in areas free of vegetation and areas treated with additional herbicide were utilised by ground nesting bees within three years of application. Nests were also evenly distributed along the vegetation-free strips underneath the apple trees. This area was an important ground-nesting bee habitat with mean numbers of nests at peak nest activity of 873 per ha (range 44-5705), and 1153 per ha (range 0-4082) in 2018 and 2019, respectively. Increasing and maintaining areas of bare ground in apple orchards during peak nesting events could improve nesting opportunities for some species of ground-nesting bee and, combined with flowers strips, be part of a more sustainable pollinator management approach. The area under the tree row is an important contributor to the ground-nesting bee habitat and should be kept bare during peak nesting.

The genome sequence of the common green furrow bee, Lasioglossum morio (Fabricius, 1793).

We present a genome assembly from an individual male Lasioglossum morio (the common green furrow bee; Arthropoda; Insecta; Hymenoptera; Halictidae). The genome sequence is 547 megabases in span. Over half of the assembly (55.79%) is scaffolded into 12 chromosomal pseudomolecules. The mitochondrial genome was also assembled, and is 16.8 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,460 protein coding genes.

Testing the systematic status of Homalictus and Rostrohalictus with weakened cross-vein groups within Halictini (Hymenoptera: Halictidae) using low-coverage whole-genome sequencing.

The halictid genus Lasioglossum, as one of the most species-rich bee groups with persistently contentious subgeneric boundaries, is one of the most challenging bee groups from a systematic standpoint. An enduring question is the relationship of Lasioglossum and Homalictus, whether all halictine bees with weakened distal wing venation comprise one or multiple genera. Here, we analyzed the phylogenetic relationships among the subgroups within Lasioglossum s.l. based on thousands of single-copy orthologs and ultraconserved elements, which were extracted from 23 newly sequenced low-coverage whole genomes alongside a published genome (22 ingroups plus 2 outgroups). Both marker sets provided consistent results across maximum likelihood and coalescent-based species tree approaches. The phylogenetic and topology test results show that the Lasioglossum and Hemihalictus series are reciprocally monophyletic and Homalictus and Rostrohalictus are valid subgenera of Lasioglossum. Consequently, we lower Homalictus to subgenus status within Lasioglossum again, and we also raise Rostrohalictus to subgenus status from its prior synonymy with subgenus Hemihalictus. Lasioglossum przewalskyi is also transferred to the subgenus Hemihalictus. Ultimately, we redefine Lasioglossum to include all halictine bees with weakened distal wing venation.

The genome sequence of the furry-claspered furrow bee, Lasioglossum lativentre (Schenck, 1853).

We present a genome assembly from an individual male Lasioglossum lativentre (the furry-claspered furrow bee; Arthropoda; Insecta; Hymenoptera; Halictidae). The genome sequence is 479 megabases in span. The majority of the assembly (75.22%) is scaffolded into 14 chromosomal pseudomolecules. The mitochondrial genome was also assembled, and is 15.3 kilobases in length.

The sweat bees from Fernando de Noronha Archipelago, Brazil (Hymenoptera: Halictidae) / Halictídeos do arquipélago de Fernando de Noronha (Hymenoptera: Halictidae)

Abstract: The sweat bees from Fernando de Noronha Archipelago are presented and illustrated herein. The species recorded are Augochlora (Augochlora) laevipyga (Kirby, 1890), Augochlora (Augochlora) sp., new record, and Lasioglossum (Dialictus) atripyga (Kirby, 1890). The Kirby species are redescribed based on recently collected specimens, including the first illustration of the male terminalia. The unidentified species is also shortly characterized.

Resumo: Os halictídeos do Arquipélago de Fernando de Noronha são aqui apresentados e ilustrados. As espécies registradas são Augochlora (Augochlora) laevipyga (Kirby, 1890), Augochlora (Augochlora) sp., novo registro, e Lasioglossum (Dialictus) atripyga (Kirby, 1890). As espécies descritas por Kirby são redescritas com base em espécimes recentemente coletados, incluindo a primeira ilustração das terminálias dos machos. A espécie não identificada também é resumidamente caracterizada.

Comparative psychophysics of colour preferences in two species of non-eusocial Australian native halictid bees.

Colour signalling by flowers appears to be the main plant-pollinator communication system observed across many diverse species and locations worldwide. Bees are considered one of the most important insect pollinators; however, native non-eusocial bees are often understudied compared to managed eusocial species, such as honeybees and bumblebees. Here, we tested two species of native Australian non-eusocial halictid bees on their colour preferences for seven different broadband colours with bee-colour-space dominant wavelengths ranging from 385 to 560 nm and a neutral grey control. Lasioglossum (Chilalictus) lanarium demonstrated preferences for a UV-absorbing white (455 nm) and a yellow (560 nm) stimulus. Lasioglossum (Parasphecodes) sp. showed no colour preferences. Subsequent analyses showed that green contrast and spectral purity had a significant positive relationship with the number of visits by L. lanarium to stimuli. Colour preferences were consistent with other bee species and may be phylogenetically conserved and linked to how trichromatic bees processes visual information, although the relative dearth of empirical evidence on different bee species currently makes it difficult to dissect mechanisms. Past studies and our current results suggest that both innate and environmental factors might both be at play in mediating bee colour preferences.

Flower-visiting insects of genus Melastoma (Myrtales: Melastomataceae) at the Fushan Botanical Garden, Taiwan.

BACKGROUND: We investigated the diversity and behaviour of insects that visit flowers of four native Melastoma (Family Melastomataceae) species of Taiwan and a horticultural hybrid Melastoma species at the Fushan Botanical Garden, Taiwan biweekly from May to August 2020. Visits of flower-visiting insects were classified into seven behavioural categories, based on the insects' behaviour and positions on the flower. The data are further assigned into four insect-flower interactions, namely pollination, herbivory, commensalism and neutralism. Our goal is to provide baseline data of insect-plant interactions of Melastoma, which is a common, but understudied plant genus in the country. NEW INFORMATION: A total of 1,289 visits to flowers were recorded by at least 63 insect morphospecies belonging to seven orders. The number of insect species recorded per Melastoma species ranged from 9 to 39. Visiting, sonication and passing were the three most frequently recorded types of behaviour, collectively accounting for 90.2% (n = 1,240) of the total observations. Pollination was the most dominant insect-flower interaction, accounting for 70.2% of the total observations, followed by neutralism (20.0%), herbivory (6.3%) and commensalism (3.5%). Sweat bees of the genera Lasioglossum and Maculonomia (Hymenoptera: Halictidae) are considered key pollinators to Melastoma species in Fushan Botanical Garden, based on their high number of visits and sonication behaviour. Our study provides the first list of insects that visit the flowers of all Taiwan's known Melastoma species and description of their interactions with the plants.

Joint Impacts of Drought and Habitat Fragmentation on Native Bee Assemblages in a California Biodiversity Hotspot.

Global climate change is causing more frequent and severe droughts, which could have serious repercussions for the maintenance of biodiversity. Here, we compare native bee assemblages collected via bowl traps before and after a severe drought event in 2014 in San Diego, California, and examine the relative magnitude of impacts from drought in fragmented habitat patches versus unfragmented natural reserves. Bee richness and diversity were higher in assemblages surveyed before the drought compared to those surveyed after the drought. However, bees belonging to the Lasioglossum subgenus Dialictus increased in abundance after the drought, driving increased representation by small-bodied, primitively eusocial, and generalist bees in post-drought assemblages. Conversely, among non-Dialictus bees, post-drought years were associated with decreased abundance and reduced representation by eusocial species. Drought effects were consistently greater in reserves, which supported more bee species, than in fragments, suggesting that fragmentation either had redundant impacts with drought, or ameliorated effects of drought by enhancing bees' access to floral resources in irrigated urban environments. Shifts in assemblage composition associated with drought were three times greater compared to those associated with habitat fragmentation, highlighting the importance of understanding the impacts of large-scale climatic events relative to those associated with land use change.

The complete mitogenome of Lasioglossum affine (Hymenoptera: Halictidae) and phylogenetic analysis.

The complete mitogenome of Lasioglossum affine (Hymenoptera: Halictidae) was sequenced and analyzed. The whole mitogenome is 17,352 bp (AT%=84.1%) and encodes 37 typical eukaryotic mitochondrial genes, including 13 protein-coding genes (PCGs), 22 tRNAs, two rRNAs, and an AT-rich region. Further analysis found three gene rearrangements, where trn I-Q-M â†’ trn M-I-Q, trn W-C-Y â†’ trn C-W-Y, and trn K-D â†’ trn D-K were shuffled. The phylogenetic relationships of 19 species of Hymenoptera were established using maximum-likelihood method based on 13 concatenated PCGs. The result showed that Lasioglossum affine is a sister of Lasioglossum sp. SJW-2017.

Evidence of Pollinators Foraging on Centipedegrass Inflorescences.

Turfgrasses are commonly used for lawns and as recreational surfaces in the USA. Because grasses are largely wind-pollinated, it was thought that pollinators would not forage on turfgrasses. Centipede grass (Eremochloa ophiuroides (Munro) Hack) is a warm-season turfgrass widely used in the southeastern USA. Centipede grass produces spike-like inflorescences from August to October, and little is known about whether pollinators utilize those inflorescences as pollen resources. Thus, the objective of the current study was to identify the pollinators foraging on centipede grass inflorescences. Pollinator samples were collected by (1) sweeping the insects actively foraging on centipede grass inflorescence for 30 min, (2) deploying pan traps for 24 h and (3) deploying malaise traps for 7 d. In the sweep samples, Lasioglossum spp., Bombus spp., Apis spp., Melissodes spp. and Augochlorella spp. were collected from centipede grass inflorescences. Syrphid flies were also collected in the sweep samples. The pan and malaise traps collected mostly Lasioglossum spp. The results imply that there is a critical need to conserve bee habitats and adopt nondisruptive lawn practices. Additionally, this new knowledge lays the foundation for future research to enhance our understanding of bee and syrphid behavior and the selection of host traits for improving bee foraging.

Review of the bee genus Homalictus Cockerell (Hymenoptera: Halictidae) from Fiji with description of nine new species.

The genus Homalictus Cockerell has not been taxonomically reviewed in the Fijian archipelago for 40 years. Here we redescribe the four known species and describe nine new ones, bringing the number of endemic Homalictus in Fiji to 13 species. We provide identifications keys to all species. Most of the species diversity (11 species) have their distributions over 800 m asl (meters above sea level; highlands), and with only two species under 800 m asl (lowlands). We highlight the vulnerability of the highland-restricted species to a warming climate, and document the potential extinction of one highland species. The new species described here are H. atritergus sp. nov., H. concavus sp. nov., H. groomi sp. nov., H. kaicolo sp. nov., H. nadarivatu sp. nov., H. ostridorsum sp. nov., H. taveuni sp. nov. H. terminalis sp. nov. and H. tuiwawae sp. nov.. [Zoobank URL: urn:lsid:zoobank.org:act:71318BEC-40CD-470F-A1E7-0E1FD18A6459].

Environmental barriers to sociality in an obligate eusocial sweat bee.

Understanding the ecological and environmental contexts in which eusociality can evolve is fundamental to elucidating its evolutionary origins. A sufficiently long active season is postulated to have been a key factor facilitating the transition to eusociality. Many primitively eusocial species exhibit an annual life cycle, which is thought to preclude the expression of eusociality where the active season is too short to produce successive worker and reproductive broods. However, few studies have attempted to test this idea experimentally. We investigated environmental constraints on the expression of eusociality in the obligate primitively eusocial sweat bee Lasioglossum malachurum, by transplanting nest foundresses from the south to the far north of the United Kingdom, far beyond the natural range of L. malachurum. We show that transplanted bees can exhibit eusociality, but that the short length of the season and harsher environmental conditions could preclude its successful expression. In one year, when foundresses were transplanted only after provisioning first brood (B1) offspring, workers emerged in the north and provisioned a second brood (B2) of reproductives. In another year, when foundresses were transplanted prior to B1 being provisioned, they were just as likely to initiate nesting and provisioned just as many B1 cells as foundresses in the south. However, the life cycle was delayed by approximately 7 weeks and nests suffered 100% B1 mortality. Our results suggest that short season length together with poor weather conditions represent an environmental barrier to the evolution and expression of eusociality in sweat bees.

Limited social plasticity in the socially polymorphic sweat bee Lasioglossum calceatum.

ABSTRACT: Eusociality is characterised by a reproductive division of labour, where some individuals forgo direct reproduction to instead help raise kin. Socially polymorphic sweat bees are ideal models for addressing the mechanisms underlying the transition from solitary living to eusociality, because different individuals in the same species can express either eusocial or solitary behaviour. A key question is whether alternative social phenotypes represent environmentally induced plasticity or predominantly genetic differentiation between populations. In this paper, we focus on the sweat bee Lasioglossum calceatum, in which northern or high-altitude populations are solitary, whereas more southern or low-altitude populations are typically eusocial. To test whether social phenotype responds to local environmental cues, we transplanted adult females from a solitary, northern population, to a southern site where native bees are typically eusocial. Nearly all native nests were eusocial, with foundresses producing small first brood (B1) females that became workers. In contrast, nine out of ten nests initiated by transplanted bees were solitary, producing female offspring that were the same size as the foundress and entered directly into hibernation. Only one of these ten nests became eusocial. Social phenotype was unlikely to be related to temperature experienced by nest foundresses when provisioning B1 offspring, or by B1 emergence time, both previously implicated in social plasticity seen in two other socially polymorphic sweat bees. Our results suggest that social polymorphism in L. calceatum predominantly reflects genetic differentiation between populations, and that plasticity is in the process of being lost by bees in northern populations. SIGNIFICANCE STATEMENT: Phenotypic plasticity is thought to play a key role in the early stages of the transition from solitary to eusocial behaviour, but may then be lost if environmental conditions become less variable. Socially polymorphic sweat bees exhibit either solitary or eusocial behaviour in different geographic populations, depending on the length of the nesting season. We tested for plasticity in the socially polymorphic sweat bee Lasioglossum calceatum by transplanting nest foundresses from a northern, non-eusocial population to a southern, eusocial population. Plasticity would be detected if transplanted bees exhibited eusocial behaviour. We found that while native bees were eusocial, 90% of transplanted bees and their offspring did not exhibit traits associated with eusociality. Environmental variables such as time of offspring emergence or temperatures experienced by foundresses during provisioning could not explain these differences. Our results suggest that the ability of transplanted bees to express eusociality is being lost, and that social polymorphism predominantly reflects genetic differences between populations.

Identification of 24 new microsatellite loci in the sweat bee Lasioglossum malachurum (Hymenoptera: Halictidae).

OBJECTIVE: The objective here is to identify highly polymorphic microsatellite loci for the Palaearctic sweat bee Lasioglossum malachurum. Sweat bees (Hymenoptera: Halictidae) are widespread pollinators that exhibit an unusually large range of social behaviours from non-social, where each female nests alone, to eusocial, where a single queen reproduces while the other members of the colony help to rear her offspring. They thus represent excellent models for understanding social evolution. RESULTS: 24 new microsatellite loci were successfully optimized. When amplified across 23-40 unrelated females, the number of alleles per locus ranged from 3 to 17 and the observed heterozygosities 0.45 to 0.95. Only one locus showed evidence of significant deviation from Hardy-Weinberg equilibrium. No evidence of linkage disequilibrium was found. These 24 loci will enable researchers to gain greater understanding of colony relationships within this species, an important model for the study of eusociality. Furthermore, 22 of the same loci were also successfully amplified in L. calceatum, suggesting that these loci may be useful for investigating the ecology and evolution of sweat bees in general.

Sweat bees on hot chillies: provision of pollination services by native bees in traditional slash-and-burn agriculture in the Yucatán Peninsula of tropical Mexico.

Traditional tropical agriculture often entails a form of slash-and-burn land management that may adversely affect ecosystem services such as pollination, which are required for successful crop yields. The Yucatán Peninsula of Mexico has a >4000 year history of traditional slash-and-burn agriculture, termed 'milpa'. Hot 'Habanero' chilli is a major pollinator-dependent crop that nowadays is often grown in monoculture within the milpa system.We studied 37 local farmers' chilli fields (sites) to evaluate the effects of landscape composition on bee communities. At 11 of these sites, we undertook experimental pollination treatments to quantify the pollination of chilli. We further explored the relationships between landscape composition, bee communities and pollination service provision to chilli.Bee species richness, particularly species of the family Apidae, was positively related to the amount of forest cover. Species diversity decreased with increasing proportion of crop land surrounding each sampling site. Sweat bees of the genus Lasioglossum were the most abundant bee taxon in chilli fields and, in contrast to other bee species, increased in abundance with the proportion of fallow land, gardens and pastures which are an integral part of the milpa system.There was an average pollination shortfall of 21% for chilli across all sites; yet the shortfall was unrelated to the proportion of land covered by crops. Rather, chilli pollination was positively related to the abundance of Lasioglossum bees, probably an important pollinator of chilli, as well indirectly to the proportion of fallow land, gardens and pastures that promote Lasioglossum abundance. Synthesis and applications. Current, low-intensity traditional slash-and-burn (milpa) agriculture provides Lasioglossum spp. pollinators for successful chilli production; fallow land, gardens and pasture therefore need to be valued as important habitats for these and related ground-nesting bee species. However, the negative impact of agriculture on total bee species diversity highlights how agricultural intensification is likely to reduce pollination services to crops, including chilli. Indeed, natural forest cover is vital in tropical Yucatán to maintain a rich assemblage of bee species and the provision of pollination services for diverse crops and wild flowers.

Bee Community of Commercial Potato Fields in Michigan and Bombus impatiens Visitation to Neonicotinoid-Treated Potato Plants.

We conducted a bee survey in neonicotinoid-treated commercial potato fields using bowl and vane traps in the 2016 growing season. Traps were placed outside the fields, at the field edges, and 10 and 30 m into the fields. We collected 756 bees representing 58 species, with Lasioglossum spp. comprising 73% of all captured bees. We found seven Bombus spp., of which B. impatiens was the only known visitor of potato flowers in our region. The majority of the bees (68%) were collected at the field edges and in the field margins. Blue vane traps caught almost four-times as many bees and collected 30% more species compared to bowl traps. Bee communities did not differ across trap locations but they were different among trap types. We tested B. impatiens visitation to neonicotinoid treated and untreated potato flowers in field enclosures. The amount of time bees spent at flowers and the duration of visits were not significantly different between the two treatments. Our results demonstrate that a diverse assemblage of bees is associated with an agroecosystem dominated by potatoes despite the apparent lack of pollinator resources provided by the crop. We found no difference in B. impatiens foraging behavior on neonicotinoid-treated compared to untreated plants.

Switchgrass (Panicum virgatum) Intercropping within Managed Loblolly Pine (Pinus taeda) Does Not Affect Wild Bee Communities.

Intensively-managed pine (Pinus spp.) have been shown to support diverse vertebrate communities, but their ability to support invertebrate communities, such as wild bees, has not been well-studied. Recently, researchers have examined intercropping switchgrass (Panicum virgatum), a native perennial, within intensively managed loblolly pine (P. taeda) plantations as a potential source for cellulosic biofuels. To better understand potential effects of intercropping on bee communities, we investigated visitation of bees within three replicates of four treatments of loblolly pine in Mississippi, U.S.A.: 3-4 year old pine plantations and 9-10 year old pine plantations with and without intercropped switchgrass. We used colored pan traps to capture bees during the growing seasons of 2013 and 2014. We captured 2507 bees comprised of 18 different genera during the two-year study, with Lasioglossum and Ceratina being the most common genera captured. Overall, bee abundances were dependent on plantation age and not presence of intercropping. Our data suggests that switchgrass does not negatively impact or promote bee communities within intensively-managed loblolly pine plantations.

Social polymorphism in the sweat bee Lasioglossum (Evylaeus) calceatum.

Temperate-zone socially polymorphic sweat bees (Hymenoptera: Halictidae) are ideal model systems for elucidating the origins of eusociality, a major evolutionary transition. Bees express either social or solitary behaviour in different parts of their range, and social phenotype typically correlates with season length. Despite their obvious utility, however, socially polymorphic sweat bees have received relatively little attention with respect to understanding the origins of eusociality. Lasioglossum (Evylaeus) calceatum is a widespread sweat bee that is thought to be socially polymorphic, with important potential as an experimental model species. We first determined the social phenotype of L. calceatum at three sites located at different latitudes within the UK. We then investigated sociality in detail across two years at the southernmost site. We found that L. calceatum exhibits latitudinal social polymorphism within the UK; bees were solitary at our two northern sites but the majority of nests were social at our southern site. Sociality in the south was characterised by a relatively small mean of two and 3.5 workers per nest in each year, respectively, and a small to medium mean caste-size dimorphism of 6.6 %. Foundresses were smaller in our more northern and high altitude populations. Sociality is clearly less specialised than in some closely related obligately social species but probably more specialied than other polymorphic sweat bees. Our research provides a starting point for future experimental work to investigate mechanisms underlying social polymorphism in L. calceatum.

Parallel responses of bees to Pleistocene climate change in three isolated archipelagos of the southwestern Pacific.

The impacts of glacial cycles on the geographical distribution and size of populations have been explored for numerous terrestrial and marine taxa. However, most studies have focused on high latitudes, with only a few focused on the response of biota to the last glacial maximum (LGM) in equatorial regions. Here, we examine how population sizes of key bee fauna in the southwest Pacific archipelagos of Fiji, Vanuatu and Samoa have fluctuated over the Quaternary. We show that all three island faunas suffered massive population declines, roughly corresponding in time to the LGM, followed by rapid expansion post-LGM. Our data therefore suggest that Pleistocene climate change has had major impacts across a very broad tropical region. While other studies indicate widespread Holarctic effects of the LGM, our data suggest a much wider range of latitudes, extending to the tropics, where these climate change repercussions were important. As key pollinators, the inferred changes in these bee faunas may have been critical in the development of the diverse Pacific island flora. The magnitude of these responses indicates future climate change scenarios may have alarming consequences for Pacific island systems involving pollinator-dependent plant communities and agricultural crops.