Ameliorative effects of Moringa oleifera leaf extract against arsenic induced histo-biochemical alterations in Labeo rohita.

The current study evaluated the efficacy of Moringa oleifera leaf extract in mitigating the histo-biochemical alterations in Labeo rohita caused by arsenic. A medical plant (Moringa oleifera) known for its numerous pharmacological qualities, was added to three different diets at 0, 2, and 4 % level, prepared by mixing M. oleifera leaf extract with the basal diet. The 96 hr lethal concentration of arsenic to Labeo rohita was 20.25 mg L-1. One hundred and eighty healthy individuals of Labeo rohita were divided into four groups. One group served as control and other three groups were subjected to sub-lethal concentration 4.05 mg L-1 (1/5th of LC50) of arsenic, with or without Moringa oleifera leaf extract supplementation for 28 days. Fish exposed to arsenic experienced significant histological alterations, higher cortisol levels, impaired antioxidant status, elevated liver enzymes (ALT, AST, and ALP), and upregulated relative expression of the cytochrome P450 gene.". But, in fish fed with diets containing 2 % or 4 % M. oleifera leaf extract, the histological alterations were reduced, level of liver enzymes, cortisol and the upregulation of anti-oxidant enzyme and cytochrome P450 gene expression was normalized, with (4 %) M. oleifera leaf extract supplemented diet exhibiting stronger effects. These results suggest the protective and therapeutic roles of M. oleifera as a feed supplement in Labeo rohita against arsenic induced toxicity.

Development and Assessment of a Multipurpose Herbal Cream With Moringa oleifera Lam.

BACKGROUND: Moringa oleifera Lam. (Moringaceae) is an indigenous tree extensively used as a nutrient and an antioxidant. In addition to its medicinal attributes, it possesses a promising role in moisturizing and cleansing the skin. OBJECTIVES: Our study aims to formulate an herbal cream from Moringa oil and evaluate its antimicrobial and anti-inflammatory activity. MATERIALS AND METHODS: In the present study, Moringa oil was extracted from the seed kernels and formulated into an herbal cream, and the antimicrobial and anti-inflammatory activity was evaluated. Among the three formulations M1, M2, and M3, the formulation M1 was homogenous, more stable, and non-greasy with constant pH. M1 was subjected to further antimicrobial studies and anti-inflammatory studies by the agar-well diffusion technique and albumin denaturation assays, respectively. RESULTS: M. oleifera seed kernel oil cream exhibited the highest antibacterial effect against Staphylococcus aureus and Escherichia coli, and the results were compared to the standard gentamycin. Moreover, M1 showed remarkable anti-inflammatory activity with an IC50 value of 124.5 µg/mL. CONCLUSION: The results show that the formulated cream will be efficient in its multipurpose activity. The current study validates the antibacterial and anti-inflammatory potential of M. oleifera seed kernel oil cream.

Green synthesis of silver, starch, and zinc oxide mediated nanoparticles with probiotics and plant extracts, their characterization and anti-bacterial activity.

Nanotechnology has various applications in all branches of science, including engineering, medicine, pharmacy, and other related fields. Conventional techniques, such as the chemical reduction approach, which produces nanoparticles (NPs) using various hazardous chemicals, offer several health risks due to their toxicity and raise serious environmental concerns. In contrast, other techniques are expensive and need a lot of energy. More than 70 % of pathogenic bacterial strains have developed resistance to at least one class of antibiotics, leading to an increase in life-threatening bacterial infections that pose a significant health risk. However, the creation of NPs by biogenic synthesis is risk-free for the environment and clean enough for biological use. This study was aimed at synthesis of novel Moringa oleifera mediated starch capped silver-zinc NPs and green synthesis of ZnO nanoparticles from Aloe vera, papaya, and Lactobacillus plantarum. Antimicrobial activity of both NPs was tested against Gram-negative antibiotic-resistant bacteria Pseudomonas aeruginosa, Gram-positive bacteria Staphylococcus aureus (ATCC 6538), and two foodborne pathogens Listeria monocytogenes and Campylobacter jejuni. Ultraviolet-visible spectroscopy, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy were used for characterization. Majority of the research studies stress the flexibility, repeatability, and desirable features of the metals, polymers, and plant components employed in the production of biomedical nanoparticles. Such an intuitive approach provides several advantages, particularly a reasonable total expense, compliance with healthcare and pharmaceutical implementations, and the ability to produce massive volumes for industrial use. The novelty of the presented work lies in the unusual combination of silver, starch, and zinc oxide nanoparticles using Moringa oleifera, which is an eco-friendly alternative to chemical-based methods. This research exhibits the formation of well-defined nanoparticles with strong antibacterial activity against a wide range of pathogens, giving us insights into their potential applications in various biomedical fields.

Symbiotic sheep milk cheese containing Moringa oleifera extract and Bifidobacterium pseudolongum INIA P2.

Healthy non-bovine functional dairy products are reaching high interest among consumers. In the present study, an aqueous polyphenol-rich Moringa oleifera extract (MoE) and a Bifidobacterium strain of human origin (B. pseudolongum INIA P2) were added, alone or in combination, for the manufacture of three experimental and one control sheep milk cheeses. In general, addition of 2.05 g of lyophilized MoE per 100 g of curd did not affect cheese dry matter or lactococci starter counts during ripening. B. pseudolongum INIA P2 showed good viability in cheese during ripening, and after simulated major gastrointestinal conditions, reaching levels above 7 log CFU / g of cheese. Cheeses with MoE showed lower pH, higher proteolysis and aminopeptidase activity than control cheese. MoE impoved functional properties, significantly (P < 0.01) increasing total phenolic content (TPC) and, especially, antioxidant capacity, with respect to control cheese. MoE modified cheese colour and volatile profile. Cheeses with MoE were darker in colour with higher red and yellow components than control cheese. Several volatile compounds were only detected in cheeses with MoE, indicating their plant origin. On top of that, increased levels of compounds originating from amino acid catabolism were present in these cheeses, as a result of their higher proteolytic and peptidolytic indexes. The symbiotic cheese with MoE and B. pseudolongum INIA P2 could confer beneficial effects on consumers' health by increasing polyphenol bioavailability and contributing to the host antioxidant capacity.

Harnessing Moringa oleifera root powder (MORP) for the sustainable remediation of heavy metal contaminated water.

Heavy metal environmental pollution is rapidly increasing due to the increase in industrialization and urbanization. Industrial processes, such as paint production, mining, and raw materials producing industries release effluents rich in heavy metals, like Pb2+, Cd2+, Cu2+, and Cr3+. These heavy metals are dangerous because they persist in nature, are non-biodegradable and they have high tendency to accumulate in the environment and in living organisms who are exposed to them. This work studied the removal of heavy metals (Cu, Pb, Cr, and Cd) from aqueous solution using Moringa oleifera root powder (MORP) as the adsorbent. The MORP was characterized by SEM, FTIR, BET, and XRD. Batch adsorption experiments carried out investigated the effects of adsorbate concentration, adsorbent dosage, agitation time, pH and temperature on adsorption. The optimum parameters are: contact time (90 min); pH (9); adsorbent dose (0.6); metal ion concentration (30 mg L-1) for Cr and 40 mg L-1 for the rest; and temperature (50 °C) for Cu and Pb, and 70 °C for Cr and Cd. These experimental data were analyzed with 5 isotherm models (Temkin, Flory-Huggins, Langmuir, D-R and Freundlich). The result obtained fitted best to Temkin isotherm in comparison to others. Kinetic studies revealed that the pseudo-second order kinetic model best described the adsorption (with high R2 values ranging from 0.9810-0.9976) compared to pseudo-first order and intra-particle diffusion kinetics model. Results of the thermodynamic study showed that the sorption process was endothermic for Cu and Pb, but exothermic for Cd and Cr. The adsorbent showed good adsorptive tendencies toward the ions studied, and could be applied on an industrial scale for the remediation of metal contaminated water.

Microalgae separation in MP-PVC contaminated wastewater using plant-based coagulant over different extraction methods in Bauru, Brazil.

This study investigates the effectiveness of coagulation-flocculation and sedimentation (CFS) for separating microalgae, focusing on the use of various Moringa oleifera extracts as natural coagulants. We examined photobioreactor effluent (PBR) both with and without microplastic PVC (MP-PVC) contamination, referred to as PBR R2 and PBR R1, respectively. Utilising response surface methodology, we identified optimal conditions for the removal of microalgae and MP-PVC. Validation tests demonstrated that the aqueous extract of delipidated Moringa oleifera powder (AEDMOP) achieved high removal efficiencies, with coagulant dosages of 630 mg L-1 for PBR R1 and 625 mg L-1 for PBR R2. Both conditions showed microalgae removal efficiencies exceeding 83% for turbidity, colour, OD540 nm, OD680 nm, and OD750 nm, and 63% for OD254 nm. Interestingly, the optimised conditions for PBR R2 required slightly less coagulant, likely due to the additional particulate matter from MP-PVC. While extracellular polymeric substances (EPS) exhibited a marginal effect on flocculation, further investigation into their role in CFS is necessary. Our findings highlight the potential of AEDMOP for efficient microalgae separation, even in the presence of microplastics.

Effect of Lactobacillus acidophilus, Calcium, and Moringa oleifera leaves extract co-administration can prevent chemical-induced carcinogenesis.

BACKGROUND: Colon cancer is the fourth leading cause of cancer deaths worldwide. The present study evaluated the chemopreventive effect of the combined treatment of Lactobacillus acidophilus, calcium citrate, and Moringa oleifera leaves extract against DMH (1,1-dimethylhydrazine hydrochloride) induced colon cancer. MATERIAL AND METHODS: Sprague Dawley rats were grouped into 10 different groups and treated with DMH 30 mg/kg s.c. for 8 weeks, Successful induction of colon cancer was confirmed with the help of symptoms, Individual and combined treatments of Lactobacillus acidophilus (109 cfu p.o.), calcium citrate (2 mg/kg p.o.) and Moringa oleifera (100 & 200 mg/kg p.o.) leaves extracts were used for 4 weeks. RESULT: After 4 weeks of treatment, it was observed that a significant reduction in aberrant crypt foci (ACFs) count, whereas liver and kidney function, AST (aspartate transaminase), ALT (alanine transaminase), urea and creatinine biomarkers were retained in its normal range. Upon DMH treatment, liver tissue loses histoarchitecture with mononuclear cell infiltration, nuclear enlargement, and hyperchromasia, this reverts due to the combined treatment of Lactobacillus acidophilus (LA), calcium citrate (CC) and hydroalcoholic extract of Moringa oleifera leaves (ME). From the results, it was revealed that individual and combined treatment of Lactobacillus acidophilus, calcium citrate, and hydroalcoholic extract of Moringa oleifera leaves shows beneficial effects against the carcinogen. CONCLUSION: Combined treatment of Lactobacillus acidophilus, calcium citrate, and hydroalcoholic extract of Moringa oleifera leaves showed positive effects against carcinogenesis and lowered aberrant crypt foci count and shows histoarchitectural improvements in liver histology with no nuclear enlargement and hyperchromasia in liver tissue.

Microwave-Assisted vs. Conventional Extraction of Moringa oleifera Seed Oil: Process Optimization and Efficiency Comparison.

This study aims to evaluate the effectiveness of microwave-assisted and conventional extraction using ethanol, hexane, and petroleum ether as solvents, and to optimize the process for extracting oil from Moringa oleifera Lam. seeds, with a focus on improving food-grade oil production. Response surface methodology (RSM) was applied to enhance the extraction process of the oil. Central composite rotational design (CCRD) was used to analyze the impact of solid-liquid ratio (x1), power (x2), and temperature (x3) on oil yield. The optimization identified the optimal conditions as a solid/liquid ratio of 1:38, power of 175 W, and temperature of 50 °C, achieving a 42% oil yield. Notably, the microwave-assisted extraction reduced the processing time from 8 h (using conventional Soxhlet extraction) to just 1 h. Conventional extraction with hexane and petroleum ether was also performed for comparison, resulting in similar oil content and fatty acid profiles, predominantly, oleic acid. FTIR analysis confirmed that the microwave-extracted oil contained fatty acids and had similar characteristics to the conventionally extracted oil. Thus, the use of ethanol as a green solvent in the microwave has shown significant improvement in terms of time and energy savings compared to the Soxhlet method with toxic solvents. This study concludes that microwave-assisted extraction with ethanol provides a more energy efficient, environmentally friendly, and time-saving alternative for food-grade oil production, aligning with advancements in food engineering and production.

Exploring the Phytochemical, Pharmacological and Nutritional Properties of Moringa oleifera: A Comprehensive Review.

Background: Moringa oleifera is one of the most nutrient-packed species, commonly known as the drumstick tree or miracle tree, and has garnered substantial popularity for its health benefits, phytochemical profile, and therapeutic potential. Moringa oleifera is a plant that is native to the Indian subcontinent and has been used in traditional medicine for thousands of years owing to its nutritional and therapeutic properties. Methods: The leaves, seeds, pods, roots, and flowers of this plant are enriched with a wide range of bioactive compounds such as flavonoids, alkaloids, vitamins, minerals, and essential amino acids. Therefore, it is considered a reservoir crop for both nutritional and medicinal applications. Result: The recent rediscovery of the plant is at the forefront of changes in nutrition, medicine, and public health. Owing to its varied clinical applications, the plant is a potential candidate for research in new drug development and functional foods. Conclusions: Potential applications of Moringa compounds in the treatment of chronic diseases include antioxidant, anti-inflammatory, antimicrobial (bacterial or fungal), and anticancerous effects. In this review, various phytochemical extraction techniques, therapeutic properties, and applications are discussed.

Evaluation of anti-Toxoplasma effects of solid lipid nanoparticles carrying Cinnamon zeylanicum and Moringa oleifera oil extracts.

BACKGROUND: The fabrication of anti-Toxoplasma drugs with less side effects and desirable efficacy is one of the important research goals facing with toxoplasmosis. This study aimed to determine the anti-Toxoplasma effects of Cinnamon zeylanicum (CZ), Moringa oleifera (MO) oil encapsulated into solid lipid nanoparticles (SLNs). METHODS: Vero cells were cultured with serial concentrations (1 mg/mL to 100 µg/mL) of CZ-SLNs and MO-SLNs in DMEM culture medium. The morphological, physical, and chemical features of nanoparticles were calculated. The cell viability assays and anti-T. gondii effects of CZ-SLNs and MO-SLNs were evaluated. The CC50 and IC50 indices of SLNs-enveloped extracts were calculated. RESULTS: The particle sizes of MO-SLNs and CZ-SLNs were 411.5 and 365 nm, while PDI indices were 0.53 and 0.7, respectively. Transmission electron microscopy (TEM) showed that both MO-SLNs and CZ-SLNs were smoothed spherical nanoparticles with rounded edges. The cytotoxicity assay showed the CC50 value of MO-SLNs at concentrations of ˃10 mg/mL. In addition, 60% of T. gondii-infected Vero cells remained alive at the concentrations ≤ 1 mg/ml, while the MO-SLNs killed at least 90% of T. gondii tachyzoites with an IC50 > 1 µg/ml. The cytotoxicity of CZ-SLNs extract showed the CC50 at the concentration 0.1 mg/mL. More than 50% of Vero cells, infected with T. gondii tachyzoites, survived at a concentration less than 0.1 mg/mL (IC50 ˂ 0.1 mg/mL), while the CZ-SLNs killed at least 85% of T. gondii tachyzoites in all concentrations. CONCLUSION: The current results represented that the use of SLNs as a nano-carrier for M. oleifera and C. zeylanicum could kill T. gondii tachyzoites with low cytotoxicity, suggesting the effectiveness of these nano-emulsions along with the chemical agents in the treatment of Toxoplasma.

Development of the Pipette-Tip Micro-Solid-Phase Extraction for Extraction of Rutin From Moringa oleifera Lam. Using Activated Hollow Carbon Nanospheres as Sorbents.

Herein, a micro-solid-phase extraction (µSPE) method was developed using a pipette tip for rutin extraction, employing activated hollow carbon nanospheres (HCNSs) as the sorbent. Characterization of the activated carbon nanospheres through TGA, FTIR, and SEM analysis confirmed the success of the activation process. The study demonstrated the efficacy of PT-µSPE in rutin extraction under pH 2 conditions with a standard concentration of 2 mg·L-1. The optimal mass of HCNSs was found to be 2 mg, and a loading volume of 500 µL resulted in the maximum recovery of rutin. Propan-2-ol was the best elution solvent with 15 aspirating/dispensing cycles. The correlation of determination (R 2) for the calibration curve was found to be 0.9991, and the LOD and LOQ values were 0.604 and 1.830 mg·L-1, respectively. The applicability of the method was demonstrated by extracting rutin from a complex Moringa oleifera leaf extract with the relative standard deviation (RSD) of 3.26%, thereby validating this method as feasible for the extraction of useful bioactive compounds from complex plant samples.

Combating Spodoptera frugiperda (Lepidoptera: Noctuidae) with Moringa-Synthesized Silica Nanoparticles and Its Combination with Some Insecticides.

The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is a major agricultural pest known for developing resistance to insecticides. This study investigated a novel approach to manage FAW by silica nanoparticles (SiNPs) synthesized from eco-friendly leaf extract of Moringa oleifera Lam. (Moringaceae). This green synthesis method offers a sustainable and potentially safer alternative to traditional chemical processes. SiNP formation was confirmed by various techniques: UV-visible spectrophotometer, X-ray spectroscopy with energy-dispersive (EDX), scanning electron microscopy (SEM), and dynamic light scattering (DLS). The effectiveness of SiNPs alone and their combination with three common insecticides (emamectin benzoate, indoxacarb, and chlorpyrifos) were evaluated against third instar larvae of fall armyworm. While SiNPs after 24 h by leaf dipping method recorded limited insecticidal activity (LC50 = 9947.59 mg/L), it significantly enhanced the potency of all three insecticides. Combining SiNPs with emamectin benzoate resulted in the most dramatic increase in effectiveness compared to the insecticide alone with LC50 = 0.295 mg/L and 0.42 mg/L, respectively. This research suggests that moringa extract can be a valuable resource for the green synthesis of nanoparticles potentially useful in pest control. This approach could potentially reduce the amount of insecticide needed for effective pest control, leading to a more sustainable and environmentally friendly agricultural practice.

Hypoglycemic Assessment of Aqueous Leaf Extract of Moringa oleifera on Diabetic Wistar Rats.

Background: Moringa oleifera leaf is used for diabetes due to its pharmacologic effects. Patients with hyperglycemia experience beta cell destruction. However, no research on risk awareness has been done to ascertain its safety. The present study describes the antidiabetic effect of Moringa oleifera leaf, such as the protection of pancreatic beta cells and the induction of glycogen synthesis, before addressing the secondary effects of diabetes, such as hepatic and renal toxicity. Methods: Forty-five Wistar rats weighed 160 ± 10 g were divided into nine groups. All animal operations complied with the National Institute of Health (NIH) guidelines for the care and use of laboratory animals as approved by the Animal Ethical Committee, University of Jos. Group I was normal control and Group II was diabetic animals induced with alloxan. Insulin and extract doses of 200, 400, and 800 mg/kg were given to diabetic Groups III-VI. Normal animals in Groups VII-IX were given extract at doses of 200, 400, and 800 mg/kg for 28 days. Tissues were retrieved for biochemical and histological investigations using standard techniques. Results: There was decrease relative body weight of diabetic animals (95.50 ± 5.50) when compared to normal control (142.75 ± 20.08) with increased levels of urea (control 6.13 ± 0.523 and diabetes 29.23 ± 1.267) and creatinine (control 0.70 ± 0.057 and diabetes 2.13 ± 0.185). Histology of the liver and pancreas also points to organ damage due to hyperglycemia. However, oral administration of extract showed antidiabetic effect with protection of pancreatic beta cells and the induction of glycogen synthesis, no glycogen was deposited in the liver, addressing the secondary effects of diabetes, such as hepatic and renal toxicity. Further discovery revealed that extract elevated antioxidant enzyme expression. Conclusion: Leaf extract from Moringa oleifera reduces blood sugar and lessens the damage caused by hyperglycemia in the pancreas and liver.

Ultrasound-assisted extraction of bioactive compounds from Moringa oleifera leaves for beef patties preservation: Antioxidant and inhibitory activities, half-life, and sensory attributes.

This study aims to examine employing ultrasound-assisted extraction of bioactive components from Moringa oleifera leaves and apply them in beef patties preservation, as well as antioxidant and inhibitory activities and sensory qualities. The study included studying the chemical content and minerals of the M. oleifera leaves, preparation of aqueous and alcoholic extracts using an ultrasound device, then exploring the extraction yield. The results proved that the extraction yield by ultrasound using ethanol at 80% was the highest, reaching 19.22%. The total phenols in the ultrasonic extract with moringa leaves aqueous extract (AMEUS) amounted to 120,755 mg/mL. Since the AMEUS exhibited the highest value of 68.308 mg/mL calic acid - eight phenolic compounds discovered by HPLC - the total content of flavonoids was also calculated. The inhibitory and antioxidant effects of moringa leaf extracts are well documented. We monitored the changes in chemical indicators, such as the value of peroxide and thiobarbituric acid, as well as the percentage of free fatty acids and physical characteristics, such as water-carrying capacity, pH, and pigments, for storage periods 0, 4, 8, and 12 days after adding AMEUS to beef patties at a concentration of 0.5%. The patties were kept under refrigeration at 4 ± 1°C during this time. The values of peroxide number, thiobarbituric acid, free fatty acid, and metmyoglobin pigment were decreased in the beef patties treated with the AMEUS. However, they increased continuously during the cryopreservation period, and there was a significant increase in water-holding capacity (WHC) when the beef patties were treated with AMEUS. The results also showed that adding AMEUS to beef patties improved their qualitative characteristics.

Corrigendum: Performance evaluation of Moringa oleifera seeds aqueous extract for removing Microcystis aeruginosa and microcystins from municipal treated-water.

[This corrects the article DOI: 10.3389/fbioe.2023.1329431.].

Pretreatment with aqueous Moringa oleifera Lam. leaf extract prevents cadmium-induced hepatotoxicity by improving cellular antioxidant machinery and reducing cadmium accumulation.

Cadmium (Cd) is a highly harmful pollutant that poses a serious threat to human health. The liver is the primary organ for Cd accumulation, and Cd-induced hepatotoxicity has been shown to be strongly correlated with an oxidative imbalance in hepatocytes. Our previous studies in the eukaryotic model organism Saccharomyces cerevisiae revealed that not only co-treatment but also pretreatment with aqueous Moringa oleifera Lam. leaf extract (AMOLE) effectively mitigated Cd toxicity by reducing intracellular Cd accumulation and Cd-mediated oxidative stress. In this study, we therefore investigated the preventive effect of AMOLE against Cd toxicity in human HepG2 hepatocytes. The results showed that, similar to the case of the yeast model, pretreatment with AMOLE prior to Cd exposure also significantly inhibited Cd-induced oxidative stress in HepG2 cells. Untargeted LC-MS/MS-based metabolomic analysis of AMOLE revealed that its major phytochemical constituents were organic acids, particularly phenolic acids and carboxylic acids. Additionally, DPPH-HPTLC fingerprints suggested that quercetin and other flavonoids possibly contribute to the antioxidant activities of AMOLE. Based on our findings, it appears that pretreatment with AMOLE prevented Cd-induced hepatotoxicity via three possible mechanisms: i) direct elimination of free radicals by AMOLE antioxidant compounds; ii) upregulation of antioxidant defensive machinery (GPx1, and HO-1) via Nrf2 signaling cascade to improve cellular antioxidant capacity; and iii) reduction of intracellular Cd accumulation, probably by suppressing Cd uptake. These data strongly suggest the high potential of AMOLE for clinical utility in the prevention of Cd toxicity.

Phytochemical screening, antimicrobial, antioxidant, anticancer activities on cervical cancer cell lines and aero-digestive extract of Moringa oleifera.

The phytochemical screening showed that the Moringa oleifera (MO) extract contained many compounds such as polyphenols, polyterpenes, sterols, reducing sugars, and hydrolysates tannins. The MICs of MO extract for microbial strains is 0.73 mg/ml for Escherichia coli, Pseudomonas aeruginosa, 7.5 mg/ml for Enterococcus faecalis, Staphylococcus aureus and 0.5 mg/ml for Candida albicans. The MO extract has an IC50 of 3.403 mg/ml has an antioxidant activity by DPPH radical scavenging. The cytotoxic activity of MO extract was evaluated by determining the content of lactate dehydrogenase released by the lysed cells. MO extract exhibited cytotoxic activity against HeLa and FaDu cell lines with an identical IC50 value of 25 µg/ml. We did not observe any remarkable decrease cytotoxic activity when these lines were exposed to the MO extract after 48 h. Our findings help to support the promising role of MO as anticancer agent and open a new challenge for studying DNA fragmentation.

In Vitro Antioxidant, Anti-Inflammatory Activity and Bioaccessibility of Ethanolic Extracts from Mexican Moringa oleifera Leaf.

This study aimed to assess the antioxidant and anti-inflammatory properties, and bioaccessibility of Moringa oleifera ethanolic extracts using pressurized liquid extraction with varying ethanol concentrations (0%, 30%, 50%, 70%, and 100%) in water-ethanol mixtures. Quercetin derivatives and neochlorogenic acid were identified as major compounds via high-performance liquid chromatography with diode array detection. The 70% ethanol extract displayed the highest antioxidant activity and phenolic content, highlighting a strong correlation between phenolics and antioxidant potential. Extracts prepared with 50% and 70% ethanol (30 µg/mL) significantly inhibited TNF-α, IL-1ß, and IL-6 cytokine secretion, with the 70% ethanol extract demonstrating robust anti-inflammatory effects. During in vitro digestion (oral, gastric, and intestinal phases), minimal changes were noted in most phenolic compounds' post-oral phase, but reductions occurred after the gastric phase. Substantial decreases in major compounds and antioxidant activity were observed in post-gastric and intestinal phases. Overall, ethanolic extracts of Moringa oleifera, particularly those with 70% ethanol, exhibit promising antioxidant and anti-inflammatory properties, suggesting potential for developing therapeutic agents against oxidative stress and inflammation-related disorders. However, it is essential to protect these compounds to prevent their degradation during digestion.

Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens.

Today, pathogenic microorganisms are increasingly developing resistance to conventional drugs, necessitating the exploration of alternative strategies. In addressing this challenge, nano-based antibacterial agents offer a promising avenue of research. In the present study, we used an extract of Moringa oleifera, a widely recognized edible and medicinal plant, to synthesize biogenetic tellurium nanoparticles (Bio-TeNPs). Transmission electron microscopy, scanning electron microscopy, and dynamic light scattering analyses revealed that the obtained Bio-TeNPs had diameters between 20 and 50 nm, and zeta potential values of 23.7 ± 3.3 mV. Fourier-transform infrared spectroscopy and X-ray photoelectron spectroscopy revealed that the Bio-TeNPs consisted primarily of Te(0), along with some organic constituents. Remarkably, these Bio-TeNPs exhibited potent antibacterial activity against a spectrum of pathogens, including Escherichia coli, Klebsiella pneumoniae, Shigella dysenteriae, Salmonella typhimurium, Streptococcus pneumoniae, and Streptococcus agalactiae. In addition, findings from growth curve experiments, live/dead cell staining, and scanning electron microscopy observations of cell morphology demonstrated that Bio-TeNPs at a concentration of 0.07 mg/mL effectively disrupted E. coli and K. pneumoniae cells, leading to cell rupture or shrinkage. The biofilm inhibition rates of 0.7 mg/mL Bio-TeNPs against E. coli and K. pneumoniae reached 92% and 90%, respectively. In addition, 7 mg/mL Bio-TeNPs effectively eradicated E. coli from the surfaces of glass slides, with a 100% clearance rate. These outcomes underscore the exceptional antibacterial efficacy of Bio-TeNPs and highlight their potential as promising nanomaterials for combating bacterial infections.

Comparative analysis of the quality of buffalo milk fresh cheese processed with Moringa oleifera seed milk coagulant and calf rennet.

This study aimed to evaluate the impact of buffalo milk fresh cheese produced using M. oleifera seed milk coagulant and calf rennet. No significant difference was found between composition and functional characteristics (P > 0.05). M. oleifera seed milk coagulant cheese exhibited smaller pore size, a more uniform conformation and a denser network structure compared with calf rennet cheese. Moreover, hardness, adhesiveness, and chewiness of calf rennet cheese were significantly higher compared with M. oleifera seed milk coagulant cheese (P < 0.05). The storage modulus of both cheeses was greater than the loss modulus, thus indicating viscoelastic behavior. Moreover, the elastic gel formed in M. oleifera seed milk coagulant cheese exhibited superior stability. In addition, the content of phosphoserine, glutamic acid, long-chain fatty acids, medium-chain fatty acids, saturated fatty acids, monounsaturated fatty acids, aldehydes, esters, and lactones was significantly higher in M. oleifera seed milk coagulant cheese compared with calf rennet cheese (P < 0.05). In addition, a strong correlation was found between free amino acids, free fatty acid (FFA), and volatile flavor compounds. The findings of this study provide a theoretical foundation for the application of M. oleifera seed milk coagulant as a new plant milk coagulant resource in the dairy industry.