Responses of soil seed banks to drought on a global scale.

The global increase in drought frequency and intensity in large areas has potentially important effects on soil seed banks (SSBs). However, a systematic evaluation of the impact of drought on SSBs at a global scale has not yet been well understood. We evaluated the effects of drought on SSBs and identified the association key drivers in the current meta-analysis. The overall effects of drought on soil seed density and richness were weak negative and positive, respectively. Drought significantly increased soil seed density by 11.94 % in forest ecosystem, whereas soil seed richness were significantly increased in vascular plants (7.39 %). Linear mixed-effect results showed that soil seed density and richness significantly reduced as increasing drought intensity. In addition, geography (altitude) has significance in controlling the lnRR of soil seed density by altering climate (mean annual precipitation, drought) and soil properties (pH, soil organic carbon, and clay content) in the structural equation model, whereas soil seed richness was controlled by geography (altitude, and latitude) via climate (mean annual precipitation). In summary, the results suggested the size of SSBs response to drought and its relationship with drought intensity in terrestrial ecosystems, it may shed light on ecosystem restoration, succession, and management using SSBs when estimating the future drought.

Soil seed bank responses to edge effects in temperate European forests.

Aim: The amount of forest edges is increasing globally due to forest fragmentation and land-use changes. However, edge effects on the soil seed bank of temperate forests are still poorly understood. Here, we assessed edge effects at contrasting spatial scales across Europe and quantified the extent to which edges can preserve the seeds of forest specialist plants. Location: Temperate European deciduous forests along a 2,300-km latitudinal gradient. Time period: 2018-2021. Major taxa studied: Vascular plants. Methods: Through a greenhouse germination experiment, we studied how edge effects alter the density, diversity, composition and functionality of forest soil seed banks in 90 plots along different latitudes, elevations and forest management types. We also assessed which environmental conditions drive the seed bank responses at the forest edge versus interior and looked at the relationship between the seed bank and the herb layer species richness. Results: Overall, 10,108 seedlings of 250 species emerged from the soil seed bank. Seed density and species richness of generalists (species not only associated with forests) were higher at edges compared to interiors, with a negative influence of C : N ratio and litter quality. Conversely, forest specialist species richness did not decline from the interior to the edge. Also, edges were compositionally, but not functionally, different from interiors. The correlation between the seed bank and the herb layer species richness was positive and affected by microclimate. Main conclusions: Our results underpin how edge effects shape species diversity and composition of soil seed banks in ancient forests, especially increasing the proportion of generalist species and thus potentially favouring a shift in community composition. However, the presence of many forest specialists suggests that soil seed banks still play a key role in understorey species persistence and could support the resilience of our fragmented forests.

Experimental Warming Hastens Physical Dormancy Break and Germination in Tropical Fabaceae.

Climate warming may threaten the germination strategies of many plants that are uniquely adapted to today's climate. For instance, species that employ physical dormancy (PY) - the production of seeds that are impermeable to water until high temperatures break them, consequently synchronizing germination with favorable growing conditions - may find that their seeds germinate during unfavorable or potentially fatal periods if threshold temperatures are reached earlier in the year. To explore this, we subjected the seeds of five species with physical dormancy (from the genera Abrus, Bauhinia, Cassia, Albizia, and Acacia) to "mild" (+2°C) and "extreme" (+4°C) future warming scenarios and documented their germination over 2 years relative to a control treatment. Under current climatic conditions, a proportion of seeds from all five species remained dormant in the soil for 2 years. A mild warming of 2°C had little to no effect on the germination of four of the five study species. Contrastingly, an extreme warming of 4°C dramatically increased germination in all five species within the first year, indicating a reduction in their ability to persist in the soil long-term. Cassia fistula was particularly susceptible to warming, exhibiting a similar increase in germination under both mild and extreme warming relative to control. Our findings suggest that climate warming in the tropics may cause the seeds of species that rely on physical dormancy to stagger the risk of unsuccessful germination across years to leave soil seed banks prematurely - the long-term implications of which remain unknown.

Using soil seed banks to assess temporal patterns of genetic variation in invasive plant populations.

Most research on the genetics of invasive plant species has focused on analyzing spatial differences among existing populations. Using a long-established Gunnera tinctoria population from Ireland, we evaluated the potential of using plants derived from seeds associated with different soil layers to track genetic variation through time. This species and site were chosen because (1) G. tinctoria produces a large and persistent seed bank; (2) it has been present in this locality, Sraheens, for ∼90 years; (3) the soil is largely undisturbed; and (4) the soil's age can be reliably determined radiometrically at different depths. Amplified fragment length polymorphic markers (AFLPs) were used to assess differences in the genetic structure of 75 individuals sampled from both the standing population and from four soil layers, which spanned 18 cm (estimated at ∼90 years based on (210)Pb and (137)Cs dating). While there are difficulties in interpreting such data, including accounting for the effects of selection, seed loss, and seed migration, a clear pattern of lower total allele counts, percentage polymorphic loci, and genetic diversity was observed in deeper soils. The greatest percentage increase in the measured genetic variables occurred prior to the shift from the lag to the exponential range expansion phases and may be of adaptive significance. These findings highlight that seed banks in areas with long-established invasive populations can contain valuable genetic information relating to invasion processes and as such, should not be overlooked.

Self-Affinity, Self-Similarity and Disturbance of Soil Seed Banks by Tillage.

Soil seed banks were sampled in undisturbed soil and after soil had been disturbed by tillage (tine, harrow or plough). Seeds were sorted by size and shape, and counted. Size-number distributions were fitted by power law equations that allowed the identification of self-similarity and self-affinity. Self-affinity and thus non-random size-number distribution prevailed in undisturbed soil. Self-similarity and thus randomness of size-number distribution prevailed after tillage regardless of the intensity of disturbance imposed by cultivation. The values of fractal dimensions before and after tillage were low, suggesting that short-term, short-range factors govern size-number distribution of soil seed banks.