"To honor and remember": An ethical awakening to African American remains in museums.

In 2021, amid surging activism in the Movement for Black Lives, the Smithsonian Institution's possession of the remains of thousands of African Americans drew widespread attention. In response, the Smithsonian and its National Museum of Natural History undertook a series of steps to assess these remains and to develop a policy for returning as many as possible to descendants and descendant communities, under changes that would apply to all non-Native American remains in Smithsonian collections. This paper reviews the actions taken to date and the work that is still in progress or planned. I contextualize these steps more broadly within an "ethical awakening" to African American remains that have long been present, studied, and displayed in collections across the museum community in the United States, where other institutions have faced similar challenges and changes.

The antiquity and ancestral origin of humans in the americas: a five hundred year inquiry from a biological anthropology perspective.

The questions -When did humans arrive in the Americas? Who were they, or from where did they come from? -are enduring and fascinating inquiries that have been approached from different perspectives, thanks to the contributions of archaeology, biological anthropology, and linguistics, among other disciplines. As a result, and after several centuries of studies, this body of research inspired several proposed models on the peopling of the Americas. These models are not only equally unique from each other but also distinct from the current themes in recent literature. However, there is a limited and occasionally inaccurate reference to the knowledge produced in the peripheral countries. This may be attributed to differences in language, academic traditions, as well as the consequences of geopolitics and neocolonialism in science. By reviewing both the old and recent literature, my aim is to present a historical account of how biological evidence has contributed to supporting and discussing some of the broad models that were proposed to explain the peopling of the Americas. Instead of providing an exhaustive account on the models, herein I focus on critically linking evidence and discussions ranging from the early skeletal discoveries at Lagoa Santa in Brazil in the 1830s to the current challenges of integrating a large amount of disparate data and collaborating with indigenous communities in the "omics" era. Far from being fully understood, investigations into the antiquity and the ancestral origin of Native Americans are revealing that these complex questions should be addressed by combining diverse data, articulating information at finer and larger grain scales, and adopting a sensitive and respectful approach by engaging with the views of indigenous communities.

Predicting chronological age based on age-related osteophyte development in the superior and inferior vertebral centrum margins of T12-L5.

The purpose of this study was to determine the relationship between vertebral margins at individual vertebral levels between T12-L5 vertebra and chronological age and develop predictive models. Three hundred and nineteen CT scans from a medical imaging database in North America were randomly selected. Three superior and inferior vertebral margin sites were scored based on observable age-related changes. All individual vertebral margin site scores significantly correlated with age at death with Pearson r values ranging from 0.47 to 0.77. Totaling the individual vertebral margin site scores for L1-L5 improved Pearson r to 0.92. All resulting predictive models were significant, and the best models predicted age at death within 10 years 70%-76% of the time for males, females, and combined male and females. In conclusion, this study produced accurate and reliable models to predict age at death using age-related changes in the spine for a North American population.

Nothing in Excess: Physical Activity, Health, and Life World in Senegalese Fulani Male Pastoralists, a Mixed Method Approach.

OBJECTIVES: The goal of this study was to evaluate the application of the short form of the International Physical Activity Questionnaire Survey (IPAQ-SF) in the rural Senegalese Fulani pastoralist population by combining quantitative and qualitative methods. DESIGN AND PARTICIPANTS: For the quantitative method, 101 men completed the IPAQ-SF questionnaire measuring moderate, vigorous, and walking physical activity. Self-rated health, BMI, and sociodemographic variables were also collected. With regard to the qualitative methods, a total of 22 participants were recruited and interviewed. Four themes were addressed, including (i) physical activity (PA) and its definition, description, related experiences, and representations of social actors; (ii) PA and health; (iii) PA and sport; and (iv) the body and Fulani world of life (i.e., Pulaagu/Ndimaagu). RESULTS: Sahelian herders have a high level of self-reported PA and a low amount of daily sitting time. The measure of PA as proposed by the IPAQ-SF is not adapted to the Senegalese Ferlo pastoralists, mainly because this scale gives too much importance to leisure-time PA, perceived as unproductive energy expenditure, which is factually and symbolically antinomic to the Fulani lifeworld. Thus, neither intense nor moderate PA is related to self-rated health. However, sedentary lifestyles are linked to self-rated health and, therefore, to mortality and morbidity in Fulani pastoralists. Finally, walking, which is the dominant PA during transhumance and herd surveillance, is related to BMI. It therefore represents a protective factor against the occurrence of overweight and associated chronic non-communicable diseases. CONCLUSION: The mixed method approach developed in this study has shown that the IPAQ-SF is not a valid measure of PA in the population of Fulani male herders from the Ferlo region, given that unproductive energy expenditure is incompatible with the Fulani way of life, which condemns excess and immoderation.

Half a century of systematic research on heat-induced colour changes in bone - A review.

Skeletal human remains presenting heat-induced changes have been a focus of study for a long time. However, there is still a long way to go for the anthropologists to be able to fully interpret and understand these changes. Heat-induced colour modifications are one of the least understood phenomena in bone, displaying a variety of exceptions (e.g., tints of yellow, orange, blue, green, pink, and red) to the expected colour variations that bone can produce when exposed to high temperatures (i.e., ivory, brown, black, various shades of grey, and white). In addition to these, there is a lack of uniformization in the literature regarding the methods to determine the exact colourations observed and the nomenclature used, giving way to subjective descriptions. However, commitment to more objective and reliable methods is visible in more recent research. In this review, we compiled data published in the literature throughout the years to portray the state of the art regarding the potential of heat-induced colour changes for inferring the circumstances of death and the applicability of these methods in the legal framework.

An investigation of the inter and intra post-mortem microstructural change seen in an experimental series of pigs exposed to a marine environment.

The identification of marine post-mortem microstructural change in human bone tissue is valuable in forensic casework as evidence of an individual's burial history. This study examined micro-tunneling in pig-bone tissue microstructure that had been submerged in a marine environment. The objective of the experiment was to assess total distribution of post-mortem microstructural change and degree of preservation within and between individual submerged pig skeletons. 14 juvenile pig carcasses were submerged in British Columbia at 92-300 m depths, between four to eight months. Seven pigs were individually submerged within caged platforms, seven were tied to open platforms. Six bones were selected from each carcass: first rib, radius, ulna, middle-rib, tibia, and femur. Two transverse thin sections were sampled at each bone mid-shaft (n = 148) and examined using circularly polarized transmitted light. The distribution of tunnels was assessed by measuring tunnel maximum ingress and diameter at 40 locations of the peripheral cortex. All element types were impacted by peripheral tunneling from the periosteum to the central cortex. Tunnels were observed as radiating, bifurcating with no remineralization boundary, isolated and in clusters. Tunnel diameters ranged between 2.00 µm and 12.8 µm, with a 3.7 µm mean. Ingress measurements ranged between 7.5 µm and 435.8 µm with a 93.0 µm mean. Distribution of post-mortem microstructural change across skeletal elements showed the averaged maximum ingress was deeper in the uncaged (99.6 µm), when compared to caged material (78.5 µm). The averaged tunnel ingress had statistically significant differences between uncaged and caged carcasses overall (p-value=0.02). Results of the study indicate microboring is present in marine submersed mammalian bone microstructure in as little as 134 days. This informs forensic investigators of the rate of skeletal destruction and of the narrow window for forensic recoveries, particularly in an enclosed environment. Furthermore, the presence of marine microboring in bone can assist forensic practitioners to histologically interpret the environmental history of a corpse.

Covariation between wrist bone morphology and maximal range of motion during ulnar deviation and supination in extant nonhuman primate taxa.

This study investigates the maximal range of motion (ROM) during wrist deviation and forearm rotation for five different primate genera and the possible correlation with the shape of the distal ulna, triquetrum and hamate. A two-block phylogenetic partial least square analysis was performed to test this covariation in a phylogenetic context, using shape coordinates and a matrix of maximal ROM data as input data. The results show that gibbons have the highest ROM for both ulnar deviation and supination, whereas Macaca exhibited the lowest ROM for supination, and Pan had the lowest ROM for ulnar deviation. These results can be attributed to differences in locomotor behaviour, as gibbons need a large wrist mobility in all directions for their highly arboreal lifestyle, whereas Macaca and Pan need a stable wrist during terrestrial locomotion. However, we found no correlation between distal ulna/triquetrum/hamate shape and maximal ROM during ulnar deviation and supination in the different primate taxa. A larger dataset, in combination with behavioural and biomechanical studies, is needed to establish form-function relationships of the primate hand, which will aid the functional interpretation of primate fossil remains.

A test of the (hu)MANid analytical program using a native American population.

OBJECTIVES: Recently, Berg and Kenyhercz (2017) developed a free web-based software package, (hu)MANid, that classifies mandibles by ancestry and sex based on either linear or mixture discriminant analysis of 11 osteometric and six morphoscopic variables. The metric and morphoscopic variables assessed using (hu)MANid have been determined to be highly replicable, however, few external validation studies have been conducted. MATERIALS AND METHODS: This article provides a test of the (hu)MANid analytical software using an independent sample (n = 52) of Native American mandibles from the Great Lakes region to investigate the accuracy of the program for identifying this important demographic group. RESULTS: When processed using linear discriminant analysis in (hu)MANid, 82.7% of the mandibles (43/52) were correctly classified as Native American. When processed using mixture discriminant analysis in (hu)MANid, 67.3% of the mandibles (35/52) were correctly classified as Native American. The difference in accuracy between the methods is not statistically significant. DISCUSSION: Our results suggest (hu)MANid is an accurate tool for anthropologists attempting to determine whether skeletal remains are Native American when establishing forensic significance, creating a biological profile, and conducting work associated with the federal Native American Graves Protection and Repatriation Act.

Technical note: Application and potentiality of quantitative ultrasonometry for the evaluation of bone mineral density status.

The evaluation of bone mineral density (BMD) is an important task in paleopathology. Techniques commonly applied in bone quantity assessment, such as DXA or radiogrammetry (XR), suffer from several limitations when applied to skeletal remains. In recently published research, we developed a new methodology and new reference curves for the evaluation of BMD on human skeletal remains, applying for the first time Quantitative Ultrasonometry (QUS), a user-friendly, portable, and reliable clinical technique. This study aims to apply this new methodology to an archeological sample and to compare the results with those obtained through XR. We apply QUS and XR to a sample of 104 adults from Medieval Italian cemeteries. Fragility fractures were recorded. Descriptive statistics and comparisons between sexes, age-at-death cohorts, and individuals with and without fragility fractures were performed. Moreover, univariate and multivariate logistic regression models were used to define the parameters most predictive of fracture risk in past populations. The comparison between sexes showed no significant results concerning BMD parameters, whereas a decrease in BMD with increasing age is confirmed. The comparison between fracture and non-fracture individuals and the logit model demonstrated that QUS parameters, especially UBPI, are more reliable predictors of fracture risk in comparison to XR. Our results confirmed that QUS is a valuable technique that can be efficiently applied to archeological remains, also considering its portability. We also propose a modification of the previously published QUS standard curves, to easily assess osteopenia and osteoporosis in archeological material.

Burned and buried: A vibrational spectroscopy analysis of burial-related diagenetic changes of heat-altered human bones.

OBJECTIVES: The analysis of burned human remains can be very challenging due to heat-induced alterations. Occasionally, human bones present these coupled with diagenetic changes, offering even more of a challenge, since there is a lack of studies regarding interactions between both taphonomic phenomena. With this study, we aimed to assess and document the effects of inhumation on the chemical composition of both unburned and burned human skeletal remains. MATERIALS AND METHODS: We buried, for 5 years, four groups of human bone samples comprising unburned bones and bones experimentally burned at 500, 900, and 1050 °C. Periodic exhumations were carried out to collect bone samples to be analyzed through Fourier transform infrared spectroscopy in attenuated total reflectance mode, in order to calculate four chemical indexes: (1) crystallinity index (CI); (2) type B carbonates to phosphate index (BPI); (3) total carbonates (A + B) to carbonate B ratio (C/C); and (4) OH to phosphate ratio (OH/P). RESULTS: After inhumation, CI and C/C of unburned bones and bones burned at 500 °C, and BPI of bones burned at 1050 °C did not vary significantly. However, the remaining indexes showed both relevant increments and reductions throughout observations, depending on burning temperature and index. DISCUSSION: Our results suggest that diagenesis can have an effect in bone's molecular composition. However, these effects do not seem to significantly affect the conclusions that can be taken from the analysis of infrared bone spectra, at least in the case of inhumations with a duration of 5 years or less.

Reaching new heights: Testing the performance of metric approaches to estimate stature from burned skeletal remains.

Bone heat-induced changes complicate osteometric stature estimation of human remains from forensic settings. The validity of current methods has not been tested to a great extent. Our aim was to determine how precise are stature estimations obtained from three different approaches, namely by using (i) Rösing's method (Rösing 1977), (ii) a 10% shrinkage correction factor (Strzalko et al. 1972) and (iii) chemosteometry (Gonçalves et al. 2020). For this purpose, pre- and post-burned head measurements from the humerus, radius and femur were used. The sample comprised 46 human skeletons of known sex and age-at-death. These were experimentally burnt to maximum temperatures ranging from 700 to 1100°C (attained after 90-188 min) for other research purposes. Stature estimations were performed through measurements in both pre-burned and burned bones using the three approaches and based on the method of Olivier and Tissier (1975). Mean absolute differences and the relative technical errors of measurements (TEM%) between the pre-burned and the estimations were calculated. Absolute mean differences indicated that the chemosteometric approach allowed for potentially more precise stature estimations than the other two procedures. However, the TEM% was very low for all approaches (smaller or close to 1%), and stature estimations were thus well within the error margin reported by Olivier and Tissier (1975). Results suggest that the chemosteometric approach enables more accurate predictions of the actual heat-induced shrinkage of each bone thus rendering more precise stature estimations. Nonetheless, the other procedures also provided quite reliable estimations although they require confirmation that the bone is calcined.

Pedagogical access and ethical considerations in forensic anthropology and bioarchaeology.

Traditional education in biological anthropology relies primarily on hands-on, highly visual experiences. Forensic anthropologists, bioarchaeologists, and osteologists in general should aim to collaborate in developing widespread digital pedagogy suitable for our discipline, increasing digital technologies used for education and training. Considerations and suggested pathways toward a biological anthropology digital pedagogy include accommodating for varying levels of digital fluency, understanding global perspectives and cultural beliefs, equity in accessibility, ethical strategies, prioritization levels of content that should be made publicly available, appropriate platforms and forms of media for disseminating different types of content, and the necessity of multiple modalities. Using three online resources as case studies, this paper focuses on the discussion of pedagogy, access, and ethics surrounding digital osteology. These three digital tools, 3D MMS, MapMorph, and J-Skel, can be used to teach students topics ranging from human variation methods and theory to juvenile age estimation. Developing a pathway forward, we encourage the anthropology community to think critically about the desired outcome of pedagogical tools in order to properly align the framework with the intended pedagogy, level of accessibility, and ethical codes. The ideal model would aim for equitable access to training materials on a global scale. Implementing these practices can foster a more adaptable and encompassing learning experience for students and researchers in biological anthropology who may have dissimilar access to resources.

The Skeletal Remains of Soldiers from the Two World Wars: Between Identification, Health Research and Memorial Issues.

After causing mass disasters that claimed the lives of tens of thousands of soldiers from countries around the world, the two Great Wars left some of them lost and missing. In France, these corpses reside in a legal vagueness where they belong neither to forensic anthropology nor archeology. Nevertheless, the process of identification and determining the cause of death requires the use of modern forensic anthropology by applying biological profiling and DNA analysis. New genomic methods also provide insight into the health statuses of these military populations, providing new perspectives on these periods of humanitarian crisis.

Parallel evolution in human populations: A biocultural perspective.

Parallel evolution-where different populations evolve similar traits in response to similar environments-has been a topic of growing interest to biologists and biological anthropologists for decades. Parallel evolution occurs in human populations thanks to myriad biological and cultural mechanisms that permit humans to survive and thrive in diverse environments worldwide. Because humans shape and are shaped by their environments, biocultural approaches that emphasize the interconnections between biology and culture are key to understanding parallel evolution in human populations as well as the nuances of human biological variation and adaptation. In this review, we discuss how biocultural theory has been and can be applied to studies of parallel evolution and adaptation more broadly. We illustrate this through four examples of parallel evolution in humans: malaria resistance, lactase persistence, cold tolerance, and high-altitude adaptation.

The Blessed Antonio (Patrizi) from Monticiano, Sienna (Italy): Bioanthropological and Palaeohistological Considerations.

Background: A medieval mummy known as the Blessed Antonio (Patrizi) is held in the church of Saints Peter and Paul at Monticiano, Sienna, central Italy. Objectives: The aim of our investigation was to complete a biological profile of the subject, as well as to assess the impact of deterioration to the concerned remains. Methods: As a follow-up of our bioanthropological, macroscopic approach, two of the samples taken underwent rehydration, fixation, desiccation, paraffin-embedding, and staining according to standard histological techniques applied to mummified remains. Results: The body was determined to be that of an adult male, who showed some pathological changes such as dental calculus and what is suspected to be hallux valgus. The overall preservation of a skin sample revealed damage caused by a post-mortem infestation of insects, while a second, inner sample was identified as lung tissue, and revealed a case of anthracosis. Conclusions: The Blessed Antonio was an adult male, who had poor dental hygiene and was likely exposed to smoke during his lifetime. Damage observed on the remains indicated that a conservation treatment was desirable for the future preservation of the body.

The Identified Skeleton Collection of Évora: importance for forensic science and bioarchaeology in the southern inland of Portugal.

The importance of reference osteological collections is unquestionable. However, the development of methodologies is more reliable the closer the collection is socioeconomically, demographically, and genetically to the population under study. The purpose of this study is to characterize and contextualize a new Portuguese reference collection. The collection of identified skeletons from the University of Évora comprises 201 adults of both sexes and seven non-adults, deceased between 1870 and 1993 and born between 1790 and 1969. It consists almost exclusively of individuals who were born and died in inland Alentejo, being the only Portuguese collection with this characteristic. The collection was built following all current legal and ethical obligations. Due to its characteristics, the collection constitutes a fundamental tool for forensic and bioarchaeological research in the inland regions of Portugal. In addition to the possibility offered to develop and validate methodologies in both sciences, the available complete hospital archives for research make this collection unique for health studies before, during, and after the epidemiological transition.

Cribra orbitalia is correlated with the meningo-orbital foramen and is vascular and developmental in nature.

Cribra orbitalia is a phenomenon with interdisciplinary interest. However, the etiology of cribra orbitalia remains unclear. Recently, the appearance of cribra orbitalia was identified as vascular in nature. This study assessed the relationship between anatomical variation of vasculature, as determined by the presence of meningo-orbital foramina, and the presence of cribra orbitalia in 178 orbits. Cribra orbitalia was identified in 27.5% (49:178) of orbits (22.7%, 35:154 adult orbits and 58.3%, 14:24 subadult orbits) and meningo-orbital foramina were identified in 65.8% (100:152) of orbits. Among the 150 total intact adult orbits (i.e., orbital roof and posterior orbits both intact), cribra orbitalia was found in 35 (23.3%). Of these 35 occurrences of cribra orbitalia, 32 (91.4%) had the concurrent finding of a meningo-orbital foramen. However, in the absence of the meningo-orbital foramen, cribra orbitalia was only found in three sides out of the total sample of intact orbits (3:150; 2.0%). Fisher's exact test revealed that the presence of cribra orbitalia and the meningo-orbital foramen were statistically dependent variables (p = .0002). Visual evidence corroborated statistical findings-vascular impressions joined cribra orbitalia to meningo-orbital foramina. This study identifies that individuals who possess a meningo-orbital foramen are anatomically predisposed to developing cribra orbitalia. Conversely, cribra orbitalia is unlikely to occur in an individual who does not possess a meningo-orbital foramen. Thus, the antecedent of cribra orbitalia is both vascular and developmental in nature. This report represents an important advancement in the understanding of cribra orbitalia-there is an anatomical predisposition to the development of cribra orbitalia.

Anthroengineering: an independent interdisciplinary field.

In recent decades, funding agencies, institutes and professional bodies have recognized the profound benefits of transdisciplinarity in tackling targeted research questions. However, once questions are answered, the previously abundant support often dissolves. As such, the long-term benefits of these transdisciplinary approaches are never fully achieved. Over the last several decades, the integration of anthropology and engineering through inter- and multidisciplinary work has led to advances in fields such as design, human evolution and medical technologies. The lack of formal recognition, however, of this transdisciplinary approach as a unique entity rather than a useful tool or a subfield makes it difficult for researchers to establish laboratories, secure permanent jobs, fund long-term research programmes and train students in this approach. To facilitate the growth and development and witness the long-term benefits of this approach, we propose the integration of anthropology and engineering be recognized as a new, independent field known as anthroengineering. We present a working definition for anthroengineering and examples of how anthroengineering has been used. We discuss the necessity of recognizing anthroengineering as a unique field and explore potential novel applications. Finally, we discuss the future of anthroengineering, highlighting avenues for moving the field forward.

A review of musculoskeletal modelling of human locomotion.

Locomotion through the environment is important because movement provides access to key resources, including food, shelter and mates. Central to many locomotion-focused questions is the need to understand internal forces, particularly muscle forces and joint reactions. Musculoskeletal modelling, which typically harnesses the power of inverse dynamics, unites experimental data that are collected on living subjects with virtual models of their morphology. The inputs required for producing good musculoskeletal models include body geometry, muscle parameters, motion variables and ground reaction forces. This methodological approach is critically informed by both biological anthropology, with its focus on variation in human form and function, and mechanical engineering, with a focus on the application of Newtonian mechanics to current problems. Here, we demonstrate the application of a musculoskeletal modelling approach to human walking using the data of a single male subject. Furthermore, we discuss the decisions required to build the model, including how to customize the musculoskeletal model, and suggest cautions that both biological anthropologists and engineers who are interested in this topic should consider.

Testing the reliability of CT scan-based dental wear magnitude scoring.

OBJECTIVES: Digital models are now frequently used in biological anthropology (bioanthropology) research. Despite several studies validating this type of research, none has examined if the assessment of dental wear magnitude based on Computerized Tomography (CT) scans is reliable. Thus, this study aims to fill this gap and assess if dental wear magnitude scoring based on CT scans provides results consistent with scoring based on direct observation of the physical specimens. MATERIALS AND METHODS: Dental wear magnitude from 412 teeth of 35 mandibles originating from the Portuguese Muge and Sado Mesolithic shell-middens was scored. The mandibles were also CT scanned and visualized using 3D Slicer. CT scan-based scoring of dental wear magnitude was then undertaken. Two scoring rounds were undertaken for each observation method (totaling four scoring rounds) and an intra-observer error test was performed. The averaged results of the two observation methods were compared via boxplots with paired cases. RESULTS: Intra-observer error was negligible and non-significant. Scoring results are comparable between the two observation methods. Notwithstanding, some differences were found, in which CT scan assessment generally overestimates dental wear when compared to direct observation. DISCUSSION: Our results generally validate the use of CT scans in studies of dental wear magnitude. Notwithstanding several caveats relating to CT scanning and visualization limitations should be considered to avoid over or under-estimation of dental wear.