Biotemplated Fe/La-co-doped TiO2 for photocatalytic depth treatment of compressed leachate from refuse transfer station.

Compressed leachate is a contaminated liquid containing various organic and inorganic pollutants produced in municipal refuse transfer stations, which pollute soil and groundwater, posing serious risks to the environment and human health. The Environmental Technology Co., Ltd. (Shenzhen, Guangdong Province, South China) treated compressed leachate obtained from a refuse transfer station. The chemical oxygen demand (COD) (641.2 mg/L) of treated compressed leachate did not meet the wastewater quality standards in China for discharge into municipal sewers (COD ≤ 500 mg/L) and the company's design discharge requirements (COD ≤ 400 mg/L). Therefore, their further in-depth treatment is necessary. To this end, waste tobacco leaves were used as the biotemplate herein, and Fe/La-co-doped TiO2 (xFe,yLa)-TTiO2(g) was synthesized using a solvothermal-assisted biotemplating method. The photocatalytic depth treatment of compressed leachate was performed under simulated solar light using the prepared catalysts. After (3Fe,3La)-TTiO2(g) treatment, the COD of the leachate decreased from 641.2 to 280.1 mg/L, and the COD removal rate was 1.2, 1.1, and 1.6 times higher than that of pure Fe-doped, La-doped and non-biological template TiO2, respectively. Characterization confirmed that the biological template endowed the catalyst with a unique morphology and high specific surface area. Its rich activity sites are conducive to enhancing the adsorption capacity of pollutants and providing an ideal place for photocatalytic reactions. Co-doping with iron and lanthanum ions altered the band structure of TiO2 and promoted the interconversion of Fe3+/Fe2+ and La3+/La2+ during photocatalysis. First-principles density functional theory simulations demonstrated that co-doping Fe and La in TiO2 created impurity levels that facilitated the transfer of photogenerated electrons. This study provides a new purification pathway for the depth treatment of compressed leachate.

Biohybrid Flexible Sperm-like Microrobot for Targeted Chemo-Photothermal Therapy.

Magnetic micro/nanorobots are promising platforms for targeted drug delivery, and their construction with soft and flexible features has received extensive attention for practical applications. Despite significant efforts in this field, facile fabrication of magnetic microrobots with flexible structures and versatility in targeted therapy remains a big challenge. Herein, we proposed a novel universal strategy to fabricate a biohybrid flexible sperm-like microrobot (BFSM) based on a Chlorella (Ch.) cell and artificial flagella, which showed great potential for targeted chemo-photothermal therapy for the first time. In this approach, microspherical Ch. cells were utilized to construct the microrobotic heads, which were intracellularly deposited with core-shell Pd@Au, extracellularly magnetized with Fe3O4, and further loaded with anticancer drug. The magnetic heads with excellent photothermal and chemotherapeutic capability were further assembled with flexible polypyrrole nanowires via biotin-streptavidin bonding to construct the BFSMs. Based on the exquisite head-to-tail structures, the BFSMs could be effectively propelled under precessing magnetic fields and move back and forth without a U-turn. Moreover, in vitro chemo-photothermal tests were conducted to verify their performance of targeted drug delivery toward localized HeLa cells. Due to this superior versatility and facile fabrication, the BFSMs demonstrated great potential for targeted anticancer therapy.

Sporopollenin Capsules as Biomimetic Templates for the Synthesis of Hydroxyapatite and ß-TCP.

Pollen grains, with their resilient sporopollenin exine and defined morphologies, have been explored as bio-templates for the synthesis of calcium phosphate minerals, particularly hydroxyapatite (HAp) and ß-tricalcium phosphate (TCP). Various pollen morphologies from different plant species (black alder, dandelion, lamb's quarters, ragweed, and stargazer lily) were evaluated. Pollen grains underwent acid washing to remove allergenic material and facilitate subsequent calcification. Ragweed and lamb's quarter pollen grains were chosen as templates for calcium phosphate salts deposition due to their distinct morphologies. The calcification process yielded well-defined spherical hollow particles. The washing step, intended to reduce the protein content, did not significantly affect the final product; thus, justifying the removal of this low-yield step from the synthesis process. Characterisation techniques, including X-ray diffraction, scanning electron microscopy, Fourier-transform infrared spectroscopy, and thermal gravimetric analysis, confirmed the successful calcification of pollen-derived materials, revealing that calcified grains were principally composed of calcium deficient HAp. After calcination, biphasic calcium phosphate composed of HAp and TPC was obtained. This study demonstrated the feasibility of using pollen grains as green and sustainable bio-templates for synthesizing biomaterials with controlled morphology, showcasing their potential in biomedical applications such as drug delivery and bone regeneration.

Bioengineering of Cu2O structured macro-biotemplate for the ultra-efficient and selective hand-retrieval of glyphosate from agro-farms.

Glyphosate (Gly) is a massively utilized toxic herbicide exceeding its statutory restrictions, causing adverse environmental and health impacts. Engineered nanomaterials, even though are integral to remediate Gly, their practical use is limited due to time and energy driven purifications, and negative environmental impacts. Here, a 3D wide area (~1.6 ± 0.4 cm2) Cu2O nanoparticle supported biotemplate is designed using fish-scale wastes as a sustainable approach for the ultra-efficient and selective hand-remediation of Gly from real-time samples from agro-farms. While the innate metal binding and reducing ability of collagenous scales aided self-synthesis cum grafting of Cu2O, the selective binding potential of Cu2O to Gly facilitated its hand-retrieval; as assessed using optical characterizations, Fourier transform infrared spectroscopy, thermogravimetric analysis and liquid chromatography mass spectrometry. Optimization studies revealed extractions of diverse pay-loads of Gly between 0.1 µg/mL to 40 µg/mL per 80 mg biotemplate grafted with ~6.354 µg of sub-5 nm Cu2O and was exponential to the number of Cu2O@biotemplates. Even though pH and surfactant didn't have any impact on the adsorption of Gly to the Cu2O@biotemplates, increase in the ionic strength led to a drastic increase in the adsorption. Density function theory simulations unveiled the involvement of phosphonic and carboxylic groups of Gly for interaction with Cu2O with a bond length of 1.826 Å and 1.833 Å, respectively. Overall, our sustainably generated, cost-efficient, hand-retrievable Cu2O supported biotemplate can be generalized to extract diverse organophosphorus toxins from agro-farms and other sewage embodiments. SYNOPSIS: Glyphosate is an excessively applied herbicide with potent health hazards and carcinogenicity. Thus, a hand removable Cu2O-supported biotemplate to selectively and efficiently remediate glyphosate from irrigation water is developed.

Biohybrid Urchin-Like ZnO-Based Microspheres with Tunable Hierarchical Structures and Enhanced Photoelectrocatalytic Properties.

Microorganisms have attracted much attention to act as biotemplates for fabricating micro/nanostructured functional particles. However, it is still challenging to produce tunable hierarchical particles based on microorganisms with intricate architectures and superior stability. Herein, a novel strategy is developed to fabricate biohybrid urchin-like magnetic ZnO microspheres based on Chlorella (Ch.) with tunable hierarchical core-shell structures. Using Ch. cells as microspherical templates, Fe3 O4 nanoparticles and ZnO nanorod (NR) arrays are deposited in sequence to form the final biohybrid heterostructure microspheres (Ch.@Fe3 O4 @ZnO NRs). Ordered growth and structural regulation of 3D ZnO NR arrays are achieved via a facile and controllable manner. Compared with the prepared microspheres with diverse structure configurations of ZnO shells, the Ch.@Fe3 O4 @ZnO NRs possess excellent light absorption and photoelectrocatalysis performance toward tetracycline degradation (normalized apparent rate constant, k = 366.3 h-1 g-1 ), which is significantly larger than that of ZnO nanoflower/nanoparticle loaded types. It also proves that the synergistic enhancement of well-oriented ZnO NR arrays, heterojunction structures, and biomass features is the fundamental reason for outstanding photoelectrocatalytic activity. Due to the remarkable stability and versatility, this work provides abundant opportunities to construct biohybrid multilevel micro/nanostructures with significant potentials for practical applications.

Photocatalytic, Antibacterial, Cytotoxic and Bioimaging Applications of Fluorescent CdS Nanoparticles Prepared in DNA Biotemplate.

Synthesizing nanoparticles in biotemplates has been cited as one of the most promising way to obtain monodispersed inorganic nanoparticles. In this method, uniform voids in porous materials serve as hosts to confine the synthesized nanoparticles. DNA template can be described as a smart glue for assembling nanoscale building blocks. Here we investigate the photocatalytic, antibacterial, cytotoxic, and bioimaging applications of DNA capped CdS. XRD, SEM, TEM, UV-visible absorption, and photoluminescence spectra were used to study structural, morphological, and optical properties of CdS nanoparticles. Prepared CdS nanoparticles exhibit visible fluorescence. The photocatalytic activity of CdS towards Rhodamine 6G and Methylene blue are 64% and 91% respectively. A disc-diffusion method is used to demonstrate antibacterial screening. It was shown that CdS nanoparticles inhibit Gram-positive bacteria and Gram-negative bacteria effectively. DNA capped CdS shows higher activity than uncapped CdS nanoparticles. MTT cell viability assays were carried out in HeLa cells to investigate the cytotoxicity for 24 h. At a concentration 2.5 µg/ml, it shows 84% cell viability and 43% viability at 12.5 µg/ml. The calculated LC50 value is equal to 8 µg/ml. These DNA capped CdS nanoparticles were taken for an in-vitro experiment with HeLa cells to exhibit the possibility of bioimaging applications. The present study suggests that the synthesized CdS nanoparticles could be a potential photocatalyst, antibacterial agent, and biocompatible nanoparticle for bioimaging applications.

Engineering Alkaline-Stable Barley Stripe Mosaic Virus-Like Particles for Efficient Surface Modification.

Viruses and virus-like particles are powerful templates for materials synthesis because of their capacity for precise protein engineering and diverse surface functionalization. We recently developed a recombinant bacterial expression system for the production of barley stripe mosaic virus-like particles (BSMV VLPs). However, the applicability of this biotemplate was limited by low stability in alkaline conditions and a lack of chemical handles for ligand attachment. Here, we identify and validate novel residues in the BSMV Caspar carboxylate clusters that mediate virion disassembly through repulsive interactions at high pH. Point mutations of these residues to create attractive interactions that increase rod length ~2 fold, with an average rod length of 91 nm under alkaline conditions. To enable diverse chemical surface functionalization, we also introduce reactive lysine residues at the C-terminus of BSMV coat protein, which is presented on the VLP surface. Chemical conjugation reactions with this lysine proceed more quickly under alkaline conditions. Thus, our alkaline-stable VLP mutants are more suitable for rapid surface functionalization of long nanorods. This work validates novel residues involved in BSMV VLP assembly and demonstrates the feasibility of chemical functionalization of BSMV VLPs for the first time, enabling novel biomedical and chemical applications.

Recent advances and progress in biotemplate catalysts for electrochemical energy storage and conversion.

Complex structures and morphologies in nature endow materials with unexpected properties and extraordinary functions. Biotemplating is an emerging strategy for replicating nature structures to obtain materials with unique morphologies and improved properties. Recently, efforts have been made to use bio-inspired species as a template for producing morphology-controllable catalysts. Fundamental information, along with recent advances in biotemplate metal-based catalysts are presented in this review through discussions of various structures and biotemplates employed for catalyst preparation. This review also outlines the recent progress on preparation routes of biotemplate catalysts and discusses how the properties and structures of these templates play a crucial role in the final performance of metal-based catalysts. Additionally, the application of bio-based metal and metal oxide catalysts is highlighted for various key energy and environmental technologies, including photocatalysis, fuel cells, and lithium batteries. Biotemplate metal-based catalysts display high efficiency in several energy and environmental systems. Note that this review provides guidance for further research in this direction.

Enhancement of Visible-Light Photocatalytic Degradation of Tetracycline by Co-Doped TiO2 Templated by Waste Tobacco Stem Silk.

In this study, Co-doped TiO2 was synthesized using waste tobacco stem silk (TSS) as a template via a one-pot impregnation method. These samples were characterized using various physicochemical techniques such as N2 adsorption/desorption analysis, diffuse reflectance UV-visible spectroscopy, X-ray diffraction, field-emission scanning electron microscopy, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and electron paramagnetic resonance spectroscopy. The synthesized material was used for the photodegradation of tetracycline hydrochloride (TCH) under visible light (420-800 nm). No strong photodegradation activity was observed for mesoporous TiO2 synthesized using waste TSS as a template, mesoporous Co-doped TiO2, or TiO2. In contrast, Co-doped mesoporous TiO2 synthesized using waste TSS as a template exhibited significant photocatalytic degradation, with 86% removal of TCH. Moreover, owing to the unique chemical structure of Ti-O-Co, the energy gap of TiO2 decreased. The edge of the absorption band was redshifted, such that the photoexcitation energy for generating electron-hole pairs decreased. The electron-hole separation efficiency improved, rendering the microstructured biotemplated TiO2 a much more efficient catalyst for the visible-light degradation of TCH.

Designer-length palladium nanowires can be templated by the central channel of tobacco mosaic virus nanorods.

We have developed methods for the templated synthesis of palladium nanowires (Pd NWs) within the central channel of tobacco mosaic virus (TMV) nanorods of various lengths. We show that uniform 4 nm diameter Pd NWs can be produced by selective growth within these channels by including the capping reagent, poly(vinyl-pyrrolidone) (PVP30K) and reducing the metal precursor to metallic palladium with ascorbic acid. The length of the Pd NWs can be controlled either by varying the length of the nanorod templates and/or through alterations to the reaction conditions. We have also demonstrated bimetallic gold (Au)-palladium (Pd) in-situ metallization of TMV nanorods resulting in the production of Pd NWs 6 nm gold nanoparticles attached to their ends. The materials produced have many potential applications in the construction of nanoscale devices.

Biotemplate Replication of Novel Mangifera indica Leaf (MIL) for Atmospheric Water Harvesting: Intrinsic Surface Wettability and Collection Efficiency.

Water shortage has become a global crisis that has posed and still poses a serious threat to the human race, especially in developing countries. Harvesting moisture from the atmosphere is a viable approach to easing the world water crisis due to its ubiquitous nature. Inspired by nature, biotemplate surfaces have been given considerable attention in recent years though these surfaces still suffer from intrinsic trade-offs making replication more challenging. In the design of artificial surfaces, maximizing their full potential and benefits as that of the natural surface is difficult. Here, we conveniently made use of Mangifera indica leaf (MIL) and its replicated surfaces (RMIL) to collect atmosphere water. This research provides a novel insight into the facile replication mechanism of a wettable surface made of Polydimethylsiloxane (PDMS), which has proven useful in collecting atmospheric water. This comparative study shows that biotemplate surfaces (RMIL) with hydrophobic characteristics outperform natural hydrophilic surfaces (DMIL and FMIL) in droplet termination and water collection abilities. Water collection efficiency from the Replicated Mangifera indica leaf (RMIL) surface was shown to be superior to that of the Dry Mangifera indica leaf (DMIL) and Fresh Mangifera indica leaf (FMIL) surfaces. Furthermore, the wettability of the DMIL, FMIL, and RMIL was thoroughly investigated, with the apices playing an important role in droplet roll-off.

Multifunctional Gold Helix Phototheranostic Biohybrid That Enables Targeted Image-Guided Photothermal Therapy in Breast Cancer.

The preparation of multifunctional smart theranostic systems is commonly achieved through complicated strategies, limiting their biomedical applications. Spirulina platensis (SP) microalgae, as a natural helix with some of the intrinsic theranostic functionalities (e.g., fluorescent and photosensitizer pigments), not only facilitates the fabrication process but also guarantees their biosafety for clinical applications. Herein, the helical architecture of gold nanoparticles (AuNPs) based on a SP biotemplate was engineered as a safe, biodegradable, and tumor-targeted biohybrid for imaging-guided photothermal therapy (PTT) to combat triple-negative breast cancer. The quasi-spherical AuNPs were embedded throughout the SP cell (Au-SP) with minimally involved reagents, only by controlling the original morphological stability of SP through pH adjustment of the synthesis media. SP thiolation increased the localization of AuNPs selectively on the cell wall without using a reducing agent (Au-TSP). SP autofluorescence, along with the high X-ray absorption of AuNPs, was employed for dual-modal fluorescence and computed tomography (FL/CT) imaging. Furthermore, the theranostic efficacy of Au-SP was improved through a targeting process with folic acid (Au-SP@CF). High tumor inhibition effects were obtained by the excellent photothermal performance of Au-SP@CF in both in vitro and in vivo analyses. Of particular note, a comparison of the photothermal effect of Au-SP@CF with the naked SP and calcined form of Au-SP@CF not only indicated the key role of the helical architecture of AuNPs in achieving a high photothermal effect but also led to the formation of new gold microspiral biohybrids (Au-MS) over the calcination process. In short, well-controllable immobilization of AuNPs, appropriate biodegradability, good hemocompatibility, long-term biosafety, accurate imaging, high tumor suppression, and low tumor metastasis effects under laser irradiation are an array of intriguing attributes, making the proposed biohybrid a promising theranostic system for FL/CT-imaging-guided PTT.

Comparative Physicochemical and Catalytic Study of Nanocrystalline Mg-Al Hydrotalcites Precipitated with Inorganic and Organic Bases.

Synthetic Mg-Al hydrotalcites (HT) are environmentally friendly solid bases frequently applied as catalysts in base catalyzed reactions. The most common synthesis method, using NaOH as precipitant, is problematized by the possibility of introducing undesired Na contamination. Alkali-free synthesis is usually performed with NH3aq, a precipitant which is less efficient in incorporation of Mg into HT lattice. In the present work, organic bases, tetrabutylammonium hydroxide and choline hydroxide, were successfully employed as precipitating agents in a new alkali-free route of Mg-Al HT synthesis. HT solids were also obtained with inorganic bases, NH3aq and NaOH. Characterization with X-ray diffraction, elemental analysis, scanning electron microscopy, Fourier-transform infrared spectroscopy and thermogravimetry/differential scanning calorimetry, confirmed the formation of nanocrystalline HT compounds with all employed bases. HT prepared with NH3aq exhibited an Mg deficit, which was detrimental to the catalytic activity in base catalyzed reactions. The effect was attributed to the tendency of Mg2+ to form ammine complexes, a conclusion supported by quantum mechanical calculations. HT prepared with NaOH showed the highest crystallinity, which was unfavorable for catalytic application. The addition of starch to the synthesis medium provided a means by which to diminish the crystal size of all HT precipitates. Catalytic tests of the Baeyer-Villiger oxidation of cyclohexanone demonstrated that the highest yields of ε-caprolactone were obtained with fine-crystalline HT catalysts prepared with organic bases in the presence of a starch template.

Virus-Like Particle-Templated Silica-Adjuvanted Nanovaccines with Enhanced Humoral and Cellular Immunity.

Virus-like particles (VLPs) are self-assembled viral proteins that represent a superior form of antigens in vaccine formulations. To enhance immunogenicity, adjuvants, especially the aluminum salts (Alum), are essentially formulated in VLP vaccines. However, Alum only induce biased humoral immune responses that limits further applications of VLP-based vaccines. To stimulate more balanced immunity, we, herein, develop a one-step strategy of using VLPs as the biotemplates to synthesize raspberry-like silica-adjuvanted VLP@Silica nanovaccines. Hepatitis B surface antigen (HBsAg) VLPs and human papillomavirus type 18 (HPV 18) VLPs are selected as model templates. Circular dichroism (CD) and affinity analyses demonstrate that HBsAg VLPs in the nanovaccines maintain their secondary structure and immunogenicity, respectively. VLP@Silica promote silica dissolution-induced lysosomal escape and cytosolic delivery of antigens, and enhance the secretion of both Th1 and Th2 type cytokines in murine bone marrow-derived dendritic cells (BMDCs). Additionally, they could improve antigen trafficking and mediate DC activation in draining lymph nodes (DLNs). Vaccination study demonstrate that both HBsAg VLP@Silica and HPV 18 VLP@Silica nanovaccines induce enhanced antigen-specific antibody productions and T-cell mediated adaptive immune responses. This design strategy can utilize VLPs derived from a diversity of viruses or their variants as templates to construct both prophylactic and therapeutic vaccines with improved immunogenicity.

Microorganism-Templated Nanoarchitectonics of Hollow TiO2-SiO2 Microspheres with Enhanced Photocatalytic Activity for Degradation of Methyl Orange.

In this study, hollow SiO2 microspheres were synthesized by the hydrolysis of tetraethyl orthosilicate (TEOS) according to the Stober process, in which Pichia pastoris GS 115 cells were served as biological templates. The influence of the preprocessing method, the TEOS concentration, the ratio of water to ethanol, and the aging time on the morphology of microspheres was investigated and the optimal conditions were identified. Based on this, TiO2-SiO2 microspheres were prepared by the hydrothermal process. The structures and physicochemical properties of TiO2-SiO2 photocatalysts were systematically characterized and discussed. The photocatalytic activity for the degradation of methyl orange (MO) at room temperature under Xe arc lamp acting as simulated sunlight was explored. The result showed that the as-prepared TiO2-SiO2 microspheres exhibited a good photocatalytic performance.

Biotemplated CdS Nano-Aggregate Networks for Highly Effective Visible-Light Photocatalytic Hydrogen Production.

In the last few decades, many new synthesis techniques have been developed in order to obtain an effective visible-light responsive photocatalyst for hydrogen production by water splitting. Among these new approaches, the biotemplated synthesis method has aroused much attention because of its unique advantages in preparing materials with special morphology and structure. In this work, Hydrilla verticillata (L. f.) Royle was used as a biotemplate to synthesize a CdS photocatalyst. The as-synthesized sample had the microstructure of nano-scaled aggregate networks and its activity for photocatalytic hydrogen production was six times higher than that of CdS synthesized without a template in an Na2S-Na2SO3 sacrificial system. The use of Pt and PdS as cocatalysts further improved the hydrogen production rate to 14.86 mmol/g·h under visible-light (λ ≥ 420 nm) irradiation, so the hydrogen production can be directly observed by the naked eye. The results of characterization showed that the as-synthesized CdS photocatalyst has a high specific surface area and narrow band gap, which is favorable for light absorption and photocatalytic reaction. This work provides a new way to search for efficient visible-light catalysts and confirms the uniqueness of a biotemplated synthesis method in obtaining specially structured materials.

Biotemplate Fabrication of Hollow Tubular CexSr1-xTiO3 with Regulable Surface Acidity and Oxygen Mobility for Efficient Destruction of Chlorobenzene: Intrinsic Synergy Effect and Reaction Mechanism.

Developing economic and applicable catalysts with elegant chlorine resistance and organic byproduct inhibition capability is of great significance for chlorinated volatile organic compounds (Cl-VOCs) eco-friendly purification. Here, ternary CexSr1-xTiO3 catalysts with tunable surface acidity and oxygen species mobility were creatively fabricated using the hollow tubular-structured fruit hair of Platanus (FHP; a widespread greenery waste) as the scaffolding biotemplate. It is shown that the oxygen vacancy (Ov) triggered by the presence of Ce can optimize the synergy between the Lewis acid sites (LAS) and Brønsted acid sites (BAS). High concentration of Ov and BAS promotes the C-Cl cleavage of chlorobenzene (CB) and accelerates the desorption of Cl• radicals as inorganic chlorine. Simultaneously, the strong electron transfer within Ti-Ce-Sr linkage increases the acidity of LAS, resulting in the superior reducibility of Ce0.4Sr0.6TiO3 and facilitating the deep oxidation of dechlorination intermediates. Additionally, the spatial confinement of the tubular structure remarkably accelerates the CB flow rate and reduces the residence time of byproducts over the prepared catalysts. Owing to these, CB can be efficiently destructed over Ce0.4Sr0.6TiO3 with selectivity of CO2 and inorganic chlorine dramatically enhanced, respectively, approximately 16 and 21 times at 275 °C compared to those of pure SrTiO3. The present work provides a feasible and promising strategy for engineering efficient catalysts for heterogeneous thermocatalytic reactions for industrial-scale Cl-CVOC destruction.

Different Plant Sporopollenin Exine Capsules and Their Multifunctional Usage.

Sporopollenin exine capsules (SECs) are highly resistant to heat and various acids and bases. They are also cheap, highly porous, eco-friendly polymer biomaterials with stable microencapsulation capacity. Due to their strong and uniquely shaped exine layers, they can allow growth on metal oxide materials, as a biotemplate for use in different applications. In this study, first, a single SEC extraction method was applied to three different pollens from Pinus, Fraxinus excelsior, and Tilia. Scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET) analysis, and thermogravimetric/differential thermal analysis (TGA/DTA) measurements both before and after the extraction process were performed to observe changes in surface area, morphology, porous structure, and degradation properties. The protein content and removal were analyzed by elemental and spectrophotometric analyses. Then, SECs were loaded by passive and centrifuge loading for drug delivery, and the loading capacities were analyzed by Fourier transform infrared spectroscopy and spectrophotometry. The method was successful in opening the pores and maintaining the structural integrity of SECs. It was determined that the morphology and porosity affected the encapsulation efficiency. According to the loading capacities, Tilia SECs were the most efficient SECs for both loading methods. In addition, three different SECs were hydrothermally coated with cobalt and then heat-treated to obtain a metal oxide structure. A CO3O4 supercapacitor electrode constructed using CO3O4-F. excelsior SEC powder had the best surface area parameters. The electrode showed a maximum specific capacity of 473 F/g for over 3000 continuous cycles of galvanostatic charge-discharge (GCD).

The Synthesis of Protein-Encapsulated Ceria Nanorods for Visible-Light Driven Hydrogen Production and Carbon Dioxide Reduction.

1D rare earth-based nanomaterials have attracted significant attention due to their excellent photo/electro-catalytic performance. The corresponding challenge is how to synthesize shape and size-controlled nanostructures in an easy scale-up way. Herein, the authors present a facile one-step strategy to design 1D multifunctional protein-encapsulated cerium oxide nanorods (PCNRs) by utilizing bovine serum albumin as an efficient biotemplate. Remarkably, the PCNRs exhibit high chemical and interfacial adhesion stability with intriguing properties, resulting in an exceptionally high activity towards H2 evolution and CO2 reduction. The photocatalytic activity of PCNRs to produce H2 is about 10 times higher than conventional CeO2 nanorods. The incorporation of rhodamine B into the PCNRs brings unprecedentedly high photocatalytic H2 evolution rate being 123 times higher than that of conventional CeO2 nanorods. Further the presence of the -NH2 groups on the PCNRs facilitated the adsorption and activation of CO2 and efficiently suppressed the proton reduction, and as a result, the PCNRs photocatalyst is highly active in converting CO2 to CO and CH4 , with the evolution rates being 50 and 83 times higher than those of conventional CeO2 nanorods, respectively. Achieving such efficient photocatalyst is a critical step toward practical production of high-value renewable fuels using solar energy.

Construction of a Bioinspired Hierarchical BiVO4/BiOCl Heterojunction and Its Enhanced Photocatalytic Activity for Phenol Degradation.

Development of a p-n heterojunction to achieve efficient degradation of organic pollutants is a promising approach in the field of photocatalysis. Herein, BiVO4 with bioinspired hierarchical structures was prepared with the sol-gel method and combined with BiOCl nanoplates to construct a 3D/2D configuration via an in situ deposition route. The hierarchical BiVO4 served as an excellent substrate to achieve the uniform loading of BiOCl nanoplates. The obtained 3D/2D BiVO4/BiOCl hybrids exhibited significantly enhanced photocatalytic efficiency for degrading phenol under visible light irradiation, with a first-order reaction rate constant that was 9.9 and 1.9 times higher than those of hierarchical BiVO4 and the BiVO4/BiOCl hybrids without hierarchical structures, respectively. Moreover, the hierarchical BiVO4/BiOCl also displayed good photochemical stability for the degradation of phenol after three recycles. The p-n heterojunction and hierarchical structure worked together to form a spatial conductive network framework, which possessed improved visible light absorption, high specific surface area, as well as effective separation and transfer of photogenerated charge carriers.