Linking transport pathways and phosphorus distribution in a loamy soil: a case study from a North-Eastern German Stagnosol.

Heterogeneous flow pathways through the soil determine the transport of dissolved and particle-bound nutritional elements like phosphorus (P) to ground and surface waters. This study was designed to understand the spatial patterns of P in agriculturally used soils and the mechanisms causing P accumulation and depletion at the centimetre scale. We conducted dye tracer experiments using Brilliant Blue on a loamy Stagnosol in North-Eastern-Germany. The plant-available P was analysed using double lactate extraction (DL-P). The plant-available P content of the topsoil was significantly higher than that of the subsoil in all three replicates (p < 0.001). The topsoil's stained areas showed significantly higher P contents than unstained areas (p < 0.05), while the opposite was found for the subsoil. The P content varied enormously across all observed soil profiles (4 to 112 mg P kg-1 soil) and different categories of flow patterns (matrix flow, flow fingers, macropore flow, and no visible transport pathways). The P contents of these transport pathways differed significantly and followed the order: Pmatrix flow > Pfinger flow > Pno visible transport pathways > Pmacropore flow. We conclude that P tends to accumulate along flow pathways in the topsoil in the observed fertilized and tilled mineral soil. In contrast, in the subsoil at a generally lower P level, P is depleted from the prominent macroporous flow domains.

The difference in the functional water flow network between the stem and current-year root cross-sectional surfaces in Salix gracilistyla stem xylem.

The dye injection method has been applied to many species to analyze the xylem water transport pathway in trees. However, traditional dye injection methods introduced dye tracers from the surface of cut stems, including several annual rings. Furthermore, the traditional dye injection method did not evaluate radial water movement from the outermost annual rings to the inner annual rings. In this study, we assessed the difference in radial water movement visualized by an injected dye, between stem base cut and current-year root cut samples of Salix gracilistyla Miq., with current-year roots grown hydroponically. The results showed that the number of stained annual rings in the root cut samples was smaller than that in the stem cut samples, and the percentage of stained vessels in the root cut samples was significantly smaller than that in the stem base cut samples in the second and third annual rings. In the current-year root cut samples, water transport mainly occurred in the outermost rings from the current-year roots to leaves. In addition, the theoretical hydraulic conductivity of stained vessels in the stem cut samples was higher in the current-year root cut samples in the second and third annual rings. These findings indicate that the previously reported dye injection method using stem cut samples overestimated the water transport pathway in the inner part of the stems. Moreover, previous hydraulic conductivity measurement methods might not have considered the effects of radial resistance through the annual ring boundary, and they might have overestimated the hydraulic conductivity in the inner annual rings.

Characteristics and influencing factors of soil preferential flow in typical stands of Karst area in southwest China.

The study of preferential flow phenomena in Karst areas and the identification of the main factors influencing preferential flow are of great importance for the recovery of local vegetation. The distribution of the dyeing solution in the vertical and horizontal directions was examined by field staining tracer test and image processing technique. We analyzed the total dyeing area ratio, matrix flow depth, preferential flow ratio, and length index as pre-ferential flow characteristic parameters, and 14 factors affecting preferential flow using grey correlation analysis. The results showed that there were two main types of preferential flow, funnel-shaped and dendritic, with lateral water movement occurring in the soil of typical Karst stands. The mean value of the dyeing area ratio of the understory in Karst areas was 19.4%, and that of the matrix flow depth, preferential flow ratio, and length index was 4.96 cm, 62.9%, and 385.5%, respectively. Among the 14 environmental factors influencing preferential flow, the initial soil moisture content had the strongest influence on the dyeing area ratio, the available potassium content had the most significant influence on the matrix flow depth, and available phosphorous content had the most significant influence on both the preferential flow ratio and the length index. The high degree of development and spatial variability of preferential flow under typical forest stands in Karst areas was strongly influenced by physical properties such as initial soil water content, while soil nutrient also exerts important influence on preferential flow.

Mechanisms of grazing management impact on preferential water flow and infiltration patterns in a semi-arid grassland in northern China.

Grazing management is widely used to control grassland degradation in Inner Mongolia. However, the correlation between the soil physical properties, root traits, and infiltration patterns of different types of grazing management has seldom been studied. To reveal the effect of grazing management on water infiltration and preferential flow behavior, we first investigated the soil and plant properties in a grazing exclusion (19 years, GE), cold-season grazing (19 years, CG), and free-grazing grassland (19 years, FG) in a semi-arid grassland in Inner Mongolia. Dye tracer infiltration was adopted to obtain the water infiltration patterns from different types of grazing management. Finally, root biomass and root morphological traits were measured in a field experiment. The results showed that the plant height, vegetation coverage, richness index, Shannon-Wiener index, soil water content, total porosity, and mean weight diameter were higher at the GE site than at the FG site, whereas soil bulk density and sand content were lower at the GE site than at the FG site (P < 0.05). In addition, the root mean diameter, specific root length, and root mass density were higher at the GE site than at the FG site. As a result, differences in these root traits and soil and vegetation properties affected the preferential water flow behavior in the three types of grassland. The preferential flow evaluation index (PFI) of the GE, CG, and FG sites was 0.89, 0.30, and 0.15, respectively, which indicated that more obvious preferential flow occurred at the GE site than at the CG and FG sites. These findings highlight that the long-term GE enhanced plant density and root biomass, which could potentially promote the natural restoration of soil pores and preferential water infiltration. Therefore, local governments and herders should implement GE rather than other grazing management practices to prevent grassland degradation.

Determining the influencing factors of preferential flow in ground fissures for coal mine dump eco-engineering.

Ground fissures (GF), appearing in front of dumps, are one of the most obvious and harmful geological hazards in coal mining areas. Studying preferential flow and its influencing factors in the ground fissures of dumps may provide basic scientific support for understanding the rapid movement of water and vegetation restoration and reconstruction in mining areas. Based on field surveys of ground fissures, three typical ground fissures were selected in the studied dump. The morphological characteristics of preferential flow for ground fissures were determined through field dye tracing, laboratory experiments, and image processing technology. The results indicated that the lengths of the three ground fissures ranged from 104.84 cm to 120.83 cm, and the widths ranged from 2.86 cm to 9.85 cm. All of the ground fissure area densities were less than 10%, and the proportion of ground fissure surface area was small in the dump. The maximum fissure depth was 47 cm, and the minimum was 16 cm. The ground fissure widths ranged from 0 cm to 14.98 cm, and the fissure width and fissure width-to-depth ratios decreased with increasing soil depth. The stained area was greater than 90% in the 0-5 cm soil layers of the three fissures, and water movement was dominated by matrix flow. The stained width decreased from 90 cm to 20 cm with increasing soil depth. The preferential flow was mainly concentrated on both sides of the fissure, which was distributed as a "T" shape. The preferential flow stained area ratios were 27.23%, 31.97%, and 30.73%, respectively, and these values decreased with increasing soil depth. The maximum stained depths of the preferential flow among the three fissures were different, and the maximum stained depth of GF II was significantly larger than that of GF I and GF III (P < 0.05). The stained path numbers of the three fissures ranged from 0 to 49. With increasing soil depth, the stained path number first increased and then decreased. The stained path widths of the three fissures ranged from 0 cm to 90 cm. With the increase in soil depth, the stained path width decreased. The stained area ratio was significantly positively correlated with ground fissure width, the ground fissure width-to-depth ratio, soil saturated hydraulic conductivity, soil organic matter, and sand content and was significantly negatively correlated with soil water content and clay content. The stained path number was significantly positively correlated with ground fissure width, the ground fissure width-to-depth ratio, soil saturated hydraulic conductivity and soil organic matter. The stained path width was significantly positively correlated with the ground fissure width-to-depth ratio, soil saturated hydraulic conductivity, soil organic matter and sand content and was significantly negatively correlated with clay content. Plant roots could significantly increase the stained area ratio, stained path number, and width and promote the formation and development of preferential flow.

Thick roots and less microaggregates improve hydrological connectivity.

Due to human activity and global climate change, the Yellow River Delta, the youngest delta wetland in China, is suffering serious degradation. The study of hydrological connection provides new perspectives and technical support for the protection and restoration of delta wetlands. To quantify the interaction between the hydrological connection and the root-soil complex, the current study took dye-tracing experiments to examine the small-scale hydrological connectivity in soil where Phramites australis grew. The dye coverage was selected as the indicator of hydrological connectivity after preliminary analysis in this study. The main results were that (1) the strength of hydrological connectivity was negatively related to the microaggregates content, but had little to do with other soil physical properties; (2) there was a notable positive correlation between the indexes of thick root (D > 5 mm) and the dye coverage hydrological connectivity, while root biomass had little effect on hydrological connectivity; and (3) the influence of the microaggregate content dominated in the combined effect of the total surface area of the root (D > 5 mm) and the microaggregate content on hydrological connectivity in each soil layer.

Cancer inhibition mechanism of lung cancer mouse model based on dye trace method.

To minimize the incidence and mortality of cancer, dye trace method was used to explore the mechanism of drug inhibition. 60 mice were selected as the research objects and randomly divided into five groups: model group, shikonin group, aconitine group, notoginsenoside R1 group, and compound group. When establishing the model, begin to administrate the medicine by gavage. The permeability of lung barrier was measured, and H.E staining, immunohistochemical staining, and Western blot test were carried out. The results showed that the mice in model group had decreased autonomic activity, increased permeability of the lung barrier, white nodules on the lung tissue, decreased protein expression related to cell proliferation and differentiation, and decreased protein expression associated with cell proliferation and differentiation, increased expression of related proteins in cancer stem cells, and low level of cell-linked communication. And the incidence of lung cancer in the model group mice was 100%. The histopathological changes in mice were improved to varying degrees after the intervention of the three drugs. Especially in the compound group, the incidence of lung cancer decreased to 8.3%. This study demonstrated that the combination of shikonin, aconitine and notoginsenoside R1 had a good anti-cancer effect, which provided a theoretical basis for clinical research.

Development of a storm surge driven water quality model to simulate spills during hurricanes.

Hurricanes can cause widespread environmental pollution that has yet to be fully articulated. This study develops a predictive water quality model to forecast potential contamination resulting from buckled or ruptured storage tanks in coastal industrialized areas when subjected to storm surge. The developed EFDC-Storm Surge model (EFDC-SS) couples EPA's EFDC code with the SWAN-ADCIRC hurricane simulation model. EFDC-SS is demonstrated using the Houston Ship Channel in Texas as a testbed and hurricane Ike as a model hurricane. Conservative and decaying dye runs evaluated various hurricane scenarios, combined with spills released at different locations and release times. Results showed that tank locations with shorter distances to the main waterbody and lower ground elevations have a higher risk of inundation and rapid spill mass transport. It was also determined that hurricane strength and landfall location, the location of the spill, and the spill release time relative to peak surge were interdependent.

Hydraulic resistance of developing Actinidia fruit.

BACKGROUND AND AIMS: Xylem flows into most fruits decline as the fruit develop, with important effects on mineral and carbohydrate accumulation. It has been hypothesized that an increase in xylem hydraulic resistance (RT) contributes to this process. This study examined changes in RT that occur during development of the berry of kiwifruit (Actinidia deliciosa), identified the region within the fruit where changes were occurring, and tested whether a decrease in irradiance during fruit development caused an increase in RT, potentially contributing to decreased mineral accumulation in shaded fruit. METHODS: RT was measured using pressure chamber and flow meter methods, the two methods were compared, and the flow meter was also used to partition RT between the pedicel, receptacle and proximal and distal portions of the berry. Dye was used as a tracer for xylem function. Artificial shading was used to test the effect of light on RT, dye entry and mineral accumulation. KEY RESULTS: RT decreased during the early phase of rapid fruit growth, but increased again as the fruit transitioned to a final period of slower growth. The most significant changes in resistance occurred in the receptacle, which initially contributed 20 % to RT, increasing to 90 % later in development. Dye also ceased moving beyond the receptacle from 70 d after anthesis. The two methods for measuring RT agreed in terms of the direction and timing of developmental changes in RT, but pressure chamber measurements were consistently higher than flow meter estimates of RT, prompting questions regarding which method is most appropriate for measuring fruit RT. Shading had no effect on berry growth but increased RT and decreased dye movement and calcium concentration. CONCLUSIONS: Increased RT in the receptacle zone coincides with slowing fresh weight growth, reduced transpiration and rapid starch accumulation by the fruit. Developmental changes in RT may be connected to changes in phloem functioning and the maintenance of water potential gradients between the stem and the fruit. The effect of shade on RT extends earlier reports that shading can affect fruit vascular differentiation, xylem flows and mineral accumulation independently of effects on transpiration.