An overview of aortic valve anatomy: the current understanding.

The traditional view of the aortic valve and aortic root as a simple conduit for blood flow between the left ventricle and the aorta is evolving with new insights from anatomy, physiology, cell biology, and advanced imaging techniques. This article provides an overview of the changing understanding of aortic root anatomy, shedding light on the intricate structures that contribute to maintaining unidirectional blood flow and the durability of the aortic valve. From historical perspectives to contemporary microscopic details, the components of the aortic root are explored, including the sinutubular junction, aortic sinuses, valve leaflets, and interleaflet triangles. Microscopically, the aortic annulus and leaflets reveal a complex architecture that facilitates blood flow while withstanding lifetime stresses. Additionally, the clinical relevance of aortic anatomy in surgical interventions is emphasized, highlighting the importance of preserving natural anatomy and physiology. A thorough understanding of the aortic root's complexity is crucial for optimizing therapeutic approaches and improving patient outcomes, paving the way for future advancements in aortic valve repair and regeneration techniques.

Development and maturation of the fibrous components of the arterial roots in the mouse heart.

The arterial roots are important transitional regions of the heart, connecting the intrapericardial components of the aortic and pulmonary trunks with their ventricular outlets. They house the arterial (semilunar) valves and, in the case of the aorta, are the points of coronary arterial attachment. Moreover, because of the semilunar attachments of the valve leaflets, the arterial roots span the anatomic ventriculo-arterial junction. By virtue of this arrangement, the interleaflet triangles, despite being fibrous, are found on the ventricular aspect of the root and located within the left ventricular cavity. Malformations and diseases of the aortic root are common and serious. Despite the mouse being the animal model of choice for studying cardiac development, few studies have examined the structure of their arterial roots. As a consequence, our understanding of their formation and maturation is incomplete. We set out to clarify the anatomical and histological features of the mouse arterial roots, particularly focusing on their walls and the points of attachment of the valve leaflets. We then sought to determine the embryonic lineage relationships between these tissues, as a forerunner to understanding how they form and mature over time. Using histological stains and immunohistochemistry, we show that the walls of the mouse arterial roots show a gradual transition, with smooth muscle cells (SMC) forming the bulk of wall at the most distal points of attachments of the valve leaflets, while being entirely fibrous at their base. Although the interleaflet triangles lie within the ventricular chambers, we show that they are histologically indistinguishable from the arterial sinus walls until the end of gestation. Differences become apparent after birth, and are only completed by postnatal day 21. Using Cre-lox-based lineage tracing technology to label progenitor populations, we show that the SMC and fibrous tissue within the walls of the mature arterial roots share a common origin from the second heart field (SHF) and exclude trans-differentiation of myocardium as a source for the interleaflet triangle fibrous tissues. Moreover, we show that the attachment points of the leaflets to the walls, like the leaflets themselves, are derived from the outflow cushions, having contributions from both SHF-derived endothelial cells and neural crest cells. Our data thus show that the arterial roots in the mouse heart are similar to the features described in the human heart. They provide a framework for understanding complex lesions and diseases affecting the aortic root.

The anatomy of the aortic root.

The aortic root is the anatomical bridge between the left ventricle and the ascending aorta. It is made up of the aortic valve leaflets, which are supported by the aortic sinuses (of Valsalva), and the interleaflet triangles interposed between the basal attachments of the leaflets. As such, it possesses significant length, and because of the semilunar attachment of the leaflets, there is no discrete proximal border to the root. It is limited distally, nonetheless, by the supravalvar ridge, or sinutubular junction. Descriptions of the aortic root over the years have been bedeviled by accounts of a valve anulus. There are at least two rings within the root, but neither serves to support the valve leaflets, each leaflets being attached in semilunar fashion from the sinutubular junction to a basal ventricular attachment Two leaflets are supported by muscle, and the third has an exclusively fibrous attachment. The root acts as a bridging structure not only anatomically, separating the myocardial and arterial components of the left ventricular pathway, but also functionally, since its proximal and distal components can withstand considerable changes in ventricular and arterial pressures. In this review, we describe the anatomy of this crucial cardiac component, emphasizing the current problems which have arisen due to indiscriminate descriptions of a nonexistent anulus.