Revealing the mechanisms behind novel auditory stimuli discrimination: An evaluation of silent functional MRI using looping star.

Looping Star is a near-silent, multi-echo, 3D functional magnetic resonance imaging (fMRI) technique. It reduces acoustic noise by at least 25dBA, with respect to gradient-recalled echo echo-planar imaging (GRE-EPI)-based fMRI. Looping Star has successfully demonstrated sensitivity to the cerebral blood-oxygen-level-dependent (BOLD) response during block design paradigms but has not been applied to event-related auditory perception tasks. Demonstrating Looping Star's sensitivity to such tasks could (a) provide new insights into auditory processing studies, (b) minimise the need for invasive ear protection, and (c) facilitate the translation of numerous fMRI studies to investigations in sound-averse patients. We aimed to demonstrate, for the first time, that multi-echo Looping Star has sufficient sensitivity to the BOLD response, compared to that of GRE-EPI, during a well-established event-related auditory discrimination paradigm: the "oddball" task. We also present the first quantitative evaluation of Looping Star's test-retest reliability using the intra-class correlation coefficient. Twelve participants were scanned using single-echo GRE-EPI and multi-echo Looping Star fMRI in two sessions. Random-effects analyses were performed, evaluating the overall response to tones and differential tone recognition, and intermodality analyses were computed. We found that multi-echo Looping Star exhibited consistent sensitivity to auditory stimulation relative to GRE-EPI. However, Looping Star demonstrated lower test-retest reliability in comparison with GRE-EPI. This could reflect differences in functional sensitivity between the techniques, though further study is necessary with additional cognitive paradigms as varying cognitive strategies between sessions may arise from elimination of acoustic scanner noise.

Looping Star fMRI in Cognitive Tasks and Resting State.

BACKGROUND: Conventional T2 *-weighted functional magnetic resonance imaging (fMRI) is performed with echo-planar imaging (EPI) sequences that create substantial acoustic noise. The loud acoustic noise not only affects the activation of the auditory cortex, but may also interfere with resting state and task fMRI experiments. PURPOSE: To demonstrate the feasibility of a novel, quiet, T2 *, whole-brain blood oxygenation level-dependent (BOLD)-fMRI method, termed Looping Star, compared to conventional multislice gradient-echo EPI. STUDY TYPE: Prospective. PHANTOM/SUBJECTS: Glover stability QA phantom; 10 healthy volunteers. FIELD STRENGTH/SEQUENCE: 3.0T: gradient echo (GE)-EPI and T2 * Looping Star fMRI. ASSESSMENT: Looping Star fMRI was presented and compared to GE-EPI with a working memory (WM) task and resting state (RS) experiments. Temporal stability and acoustic measurements were obtained for both methods. Functional maps and activation accuracy were compared to evaluate the performance of the novel sequence. STATISTICAL TESTS: Mean and standard deviation values were analyzed for temporal stability and acoustic noise tests. Activation maps were assessed with one-sample t-tests and contrast estimates (CE). Paired t-tests and receiver operator characteristic (ROC) were used to compare fMRI sensitivity and performance. RESULTS: Looping Star presented a 98% reduction in sound pressure compared with GE-EPI, with stable temporal stability (0.09% percent fluctuation), but reduced temporal signal-to-noise ratio (tSNR) (mean difference = 15.9%). The novel method yielded consistent activations for RS and WM (83.4% and 69.5% relative BOLD sensitivity), which increased with task difficulty (mean CE 2-back = 0.56 vs. 0-back = 0.08, P < 0.05). A few differences in spatial activations were found between sequences, leading to a 4-8% lower activation accuracy with Looping Star. DATA CONCLUSION: Looping Star provides a suitable approach for whole-brain coverage with sufficient spatiotemporal resolution and BOLD sensitivity, with only 0.5 dB above ambient noise. From the comparison with GE-EPI, further developments of Looping Star fMRI should target increased sensitivity and spatial specificity for both RS and task experiments. LEVEL OF EVIDENCE: 2. TECHNICAL EFFICACY STAGE: 1 J. Magn. Reson. Imaging 2020;52:739-751.

Looping Star.

PURPOSE: To introduce a novel MR pulse sequence, termed Looping Star, for fast, robust, and yet quiet, 3D radial multi-gradient echo T2* MR imaging. METHODS: The Looping Star pulse sequence is based on the 3D radial Rotating Ultra-Fast Imaging Sequence (RUFIS) extended by a time-multiplexed gradient-refocusing mechanism. First, multiple magnetic coherences are excited, which are subsequently gradient-refocused in form of a looping k-space trajectory. Accordingly, Looping Star captures an initial FID image followed by gradient echo images at equidistant echo times. RESULTS: Looping Star was demonstrated in phantom and in vivo volunteer experiments for 3D, high resolution T2* weighted imaging, T2* mapping, and quantitative susceptibility mapping (QSM). The method is fast, quiet, and robust against imperfections including Eddy currents, motion, and geometric distortions. When applied to a motor task fMRI experiment a BOLD sensitivity of 5% was achieved at minimal acoustic noise (i.e. 2.7 dB(A) above ambient noise) and with images congruent to other anatomical scans. CONCLUSIONS: Looping Star imaging provides new and exciting opportunities for fast, robust and yet quiet T2* MR imaging. Potential applications include T2*-weighted imaging, T2* mapping, QSM, and fMRI.