Needle-Plug/Piston-Based Modular Mesoscopic Design Paradigm Coupled With Microfluidic Device for Point-of-Care Pooled Testing.

Emerging diagnostic scenarios, such as population surveillance by pooled testing and on-site rapid diagnosis, highlight the importance of advanced microfluidic systems for in vitro diagnostics. However, the widespread adoption of microfluidic technology faces challenges due to the lack of standardized design paradigms, posing difficulties in managing macro-micro fluidic interfaces, reagent storage, and complex macrofluidic operations. This paper introduces a novel modular-based mesoscopic design paradigm, featuring a core "needle-plug/piston" structure with versatile variants for complex fluidic operations. These structures can be easily coupled with various microfluidic platforms to achieve truly self-contained microsystems. Incorporated into a "3D extensible" design architecture, the mesoscopic design meets the demands of function integration, macrofluid manipulations, and flexible throughputs for point-of-care nucleic acid testing. Using this approach, an ultra-sensitive nucleic acid detection system is developed with a limit of detection of ten copies of SARS-CoV-2 per mL. This system efficiently conducts large-scale pooled testing from 50 pharyngeal swabs in a tube with an uncompromised sensitivity, enabling a truly "sample-in-answer-out" microsystem with exceptional performance.

Triphenylamine-Based Covalent Organic Framework Films for Dopamine-Responsive Electrofluorochromism.

Covalent organic framework (COF) film with electrofluorochromic (EFC) and electrochromic (EC) properties has been synthesized by using triphenylamine-based monomers. The film exhibited a high maximum fluorescence contrast of 151 when subjected to a drive voltage of 0.75 V vs the Ag/AgCl electrode, causing the fluorescence to be quenched, which resulted in the EFC process's "fluorescence off" state. The switching times for the fluorescence on and off states were 0.51 and 7.79 s, respectively. Over the same voltage range, the COF film also displayed EC properties, achieving a contrast of 50.23% and a coloration efficiency of 297.4 cm2 C-1 at 532 nm, with switching times of 18.6 s for coloration and 0.7 s for bleaching. Notably, the quenched fluorescence of the COF film could be restored by adding dopamine as a reductant. This phenomenon enabled the implementation of a NAND logic gate using the applied potential as a physical input and dopamine addition as a chemical input. This study demonstrates the successful development of COF films with bifunctional EFC and EC properties, showcasing their potential for use in constructing advanced optoelectronic devices.

Worm-Inspired, Untethered, Soft Crawling Robots for Pipe Inspections.

The increasing demand for inspection, upkeep, and repair of pipeline and tunnel infrastructures has catalyzed research into the creation of robots with superior flexibility, adaptability, and load-bearing capacities. This study introduces an autonomous soft robot designed for navigating both straight and curved pipelines of 90 mm diameter. The soft robot is enabled by an elongation pneumatic actuator (EPA) as its body and multiple radial expansion pneumatic actuators (REPAs) as its feet to provide adhesion and support on the pipe walls. It achieves a horizontal movement speed of 1.27 mm/s and ascends vertically at 0.39 mm/s. An integrated control mechanism, merging both pneumatic and electrical systems is employed to facilitate unrestrained movement. A novel control tactic has been formulated to ensure synchronized coordination between the robot's body deformation and leg anchoring, ensuring stable movement. This soft robot demonstrates remarkable mobility metrics, boasting an anchoring strength of over 100 N, a propelling force of 43.8 N when moving vertically, and a pulling strength of 31.4 N during navigation in curved pipelines. It can carry a camera to capture the internal view of the pipe and remove obstacles autonomously. The unconstrained and autonomous movement of the untethered soft robot presents new opportunities for various applications at different scales.

Tailor-Made Doses of Pharmaceuticals by Tunable Modular Design: A Case Study on Tapering Antidepressant Medication.

An abrupt cessation of antidepressant medication can be challenging due to the appearance of withdrawal symptoms. A slow hyperbolic tapering of an antidepressant, such as citalopram hydrobromide (CHB), can mitigate the withdrawal syndrome. However, there are no viable dosage forms on the market to implement the tapering scheme. A solution using a tunable modular design (TMD) approach to produce flexible and accurate doses of CHB is proposed. This design consists of two parts: 1) a module with a fixed amount of preloaded CHB in a freeze-dried polymer matrix, and 2) fine-tuning the CHB dose by inkjet printing. A noncontact food-grade printer, used for the first time for printing pharmaceuticals, is modified to allow for accurate printing of the highly concentrated CHB ink on the porous CHB-free or CHB-preloaded modules. The produced modules with submilligram precision are bench-marked with commercially available CHB tablets that are manually divided. The TMD covers the entire range of doses needed for the tapering (0.5-23.8 mg). The greatest variance is 13% and 88% when comparing the TMD and self-tapering, respectively. Self-tapering is proven inaccurate and showcases the need for the TMD to make available accurate and personalized doses to wean off treatment with CHB.

Modular Design of Functional Glucose Monomer and Block Co-Polymer toward Stable Zn Anodes.

Aqueous Zn batteries employing mildly acidic electrolytes have emerged as promising contenders for safe and cost-effective energy storage solutions. Nevertheless, the intrinsic reversibility of the Zn anode becomes a focal concern due to the involvement of acidic electrolyte, which triggers Zn corrosion and facilitates the deposition of insulating byproducts. Moreover, the unregulated growth of Zn over cycling amplifies the risk of internal short-circuiting, primarily induced by the formation of Zn dendrites. In this study, a class of glucose-derived monomers and a block copolymer are synthesized through a building-block assembly strategy, ultimately leading to uncover the optimal polymer structure that suppresses the Zn corrosion while allowing efficient ion conduction with a substantial contribution from cation transport. Leveraging these advancements, remarkable enhancements are achieved in the realm of Zn reversibility, exemplified by a spectrum of performance metrics, including robust cycling stability without voltage overshoot and short-circuiting during 3000 h of cycling, stable operation at a high depth of charge/discharge of 75% and a high current density, >95% Coulombic efficiency over 2000 cycles, successful translation of the anode improvement to full cell performance. These polymer designs offer a transformative path based on the modular synthesis of polymeric coatings toward highly reversible Zn anode.

Design and Analysis of Reconfigurable Origami-Based Vacuum Pneumatic Artificial Muscles for Versatile Robotic System.

In this study, a vacuum-based modular actuator system named reconfigurable origami-based vacuum pneumatic artificial muscles (ROV-PAMs) is presented. The system consists of six types of actuating modules and three types of fluidic supporting modules each embedded with magnet-based connectors so that the modules can be assembled to modify the system behavior. The module can be used in a myriad of ways, including extending their working range, creating complex geometries upon deformation, and cooperating to improve overall performance. A simple analytical model for the actuating modules is derived based on the law of conservation of energy, and the model is verified experimentally which shows that this intuitive model can provide a reasonable prediction of performance. A block sorting robot is built using three different types of actuating modules with multiple fluidic supporting modules, and the robot shows that it is possible to flexibly and easily assemble modules to build a robot capable of completing diverse tasks. The ROV-PAM module and its concept can be applied to realize robotic designs, which can be altered on-the-fly to adjust its functionality to meet the evolving requirements required for truly flexible automation.

Spidroin-mimetic Engineered Protein Fibers with High Toughness and Minimized Batch-to-batch Variations through ß-sheets Co-assembly.

Synthetic spidroin fibers have not yet attained the same level of toughness and stability as natural spider silks due to the complexity of composition and hierarchical structure. Particularly, understanding the intricate interactions between spidroin components in spider fiber is still elusive. Herein, we report modular design and preparation of spidroin-mimetic fibers composed of a conservative C-terminus spidroin module, two different natural ß-sheets modules, and a non-spidroin random-coil module. The resulting fibers exhibit a toughness of ~200 MJ/m3, reaching the highest value among the reported artificial spider silks. The interactions between two components of recombinant spidroins facilitate the intermolecular co-assembly of ß-sheets, thereby enhancing the mechanical strength and reducing batch-to-batch variability in the dual-component spidroin fibers. Additionally, the dual-component spidroin fibers offer potential applications in implantable or even edible devices. Therefore, our work presents a generic strategy to develop high-performance protein fibers for diverse translations in different scenarios.

Modular design of curved beam-based recyclable architected materials.

Advances in manufacturing technologies have enabled architected materials with unprecedented properties. These materials are typically irreversibly designed and fabricated with characteristic geometries and specific mechanical properties, thus rendering them suitable for pre-specified requests. However, these materials cannot be recycled or reconstructed into different shapes and functionalities to economically adapt to various environments. Hence, we present a modular design strategy to create a category of recyclable architected materials comprising elastic initially curved beams and rigid cylindrical magnets. Based on numerical analyses and physical prototypes, we introduce an arc-serpentine curved beam (ASCB) and systematically investigate its mechanical properties. Subsequently, we develop two sets of hierarchical modules for the ASCB, thus expanding the constructable shape of architected materials from regular cuboids to complex curved surfaces. Furthermore, we demonstrate that the magnets attached to the centers of specific serpentine patterns of the modules allows the effective in-situ recycling of the designed materials, including sheet materials for non-damage storage, bulk materials for tunable stiffness, and protective package boxes for reshaping into decorative lampshades. We expect our approach to improve the flexibility of architected materials for multifunctional implementation in resource-limited scenarios.

Modular 3D printed platform for fluidically connected human brain organoid culture.

Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected but spatially separated brain organoid array culture. This all-in-one platform allows all culturing steps-from cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation-to be performed in a single well plate without the need for organoid manipulation or transfer. Importantly, the approach relies on accessible materials and widely available 3D printing equipment. Furthermore, the developed design principles are modular and highly customizable. As such, we believe that the presented technology can be easily adapted by other research groups and fuel further development of culturing tools and platforms for brain organoids and other 3D cellular systems.

Exploration and evaluation of modular concepts for the design of full-scale pharmaceutical manufacturing facilities.

The design of biopharmaceutical processes is predominantly driven by the domain of experimental process design. This approach can be further improved by combining multiple domain information such as experiments, unit models, and flowsheet models. Approaches consisting of methods and flowsheet models provide the framework for exploring, analyzing, and ultimately evaluating the combinatorial space of all possible designs within the molecule-to-manufacturing value chain. In recent years, modular process designs are of interest in the pharmaceutical industry because of the shift toward multiproduct, mutiprocess processes. Therefore, a systematic approach for how to evaluate the utilization of the modular plug-n-play concept provides metrics that can propel modular design from a viable design alternative to the selected alternative for full-scale manufacturing. The objective of this paper is to present such an in silico approach for the evaluation of modular designs. The approach is presented as a systematic method and then, is exemplified through the manufacture of an active pharmaceutical ingredient (API). The application of the method shows how to transition from a typical design-for-purpose design alternative to a modular design through the utilization of data, modeling, simulation, and uncertainty/sensitivity analyses for quantification of various selection metrics such as process robustness and flexibility.

Space-technological and architectural methodology and process towards design of long-term habitats for scientific human missions on mars.

Centered on the core idea of long duration habitat design for research crew on Mars, the Martian Habitat Units (MHUs) are designed as a cluster of 10 units each with the maximum capacity of 9 crew members to live and carry on with the local challenges of scientific and exploratory life, while enjoying their lives as intellectual, social individuals in the harsh environment of Mars for durations in the order of magnitude of several years. This approach to the concept of a living environment in sharp contradiction to that of a shelter with the minimal capabilities to meet the requirements of terrestrial life to the point of survival, has led the outcoming design to be a fulfilling environment for the inhabitants of the units to evolve and thrive culturally, while being on a years-long mission. This manuscript provides detailed insight on the lessons learned of the aforementioned comprehensive design attempt with, but not limited to, the following core concerns: •The initial stand-point of such a design procedure relies on an ever increasing and comprehensive list of concerns, be it classically discussed in the literature and predictable, or unforeseen on the face of it, but to be prevented anyhow. The manuscript discusses the most crucial ones of such criteria/concerns.•The infamous saying of "Whatever that can go wrong, will go wrong" demands a rather complex level of redundancies in all layers of the design and the thought procedure behind its all aspects. The manuscript addresses the adequate steps towards its realization.•Modularity in all layers of the design plays a key role in reducing construction, maintenance, and installation costs, as for any deep space mission the mentioned expenses are astronomically high themselves. The manuscript presents our solution for geometric modularity of the design.

Modular Design in Triboelectric Sensors: A Review on the Clinical Applications for Real-Time Diagnosis.

Triboelectric nanogenerators (TENGs) have garnered considerable interest as a promising technology for energy harvesting and stimulus sensing. While TENGs facilitate the generation of electricity from micro-motions, the modular design of TENG-based modular sensing systems (TMSs) also offers significant potential for powering biosensors and other medical devices, thus reducing dependence on external power sources and enabling biological processes to be monitored in real time. Moreover, TENGs can be customised and personalized to address individual patient needs while ensuring biocompatibility and safety, ultimately enhancing the efficiency and security of diagnosis and treatment. In this review, we concentrate on recent advancements in the modular design of TMSs for clinical applications with an emphasis on their potential for personalised real-time diagnosis. We also examine the design and fabrication of TMSs, their sensitivity and specificity, and their capabilities of detecting biomarkers for disease diagnosis and monitoring. Furthermore, we investigate the application of TENGs to energy harvesting and real-time monitoring in wearable and implantable medical devices, underscore the promising prospects of personalised and modular TMSs in advancing real-time diagnosis for clinical applications, and offer insights into the future direction of this burgeoning field.

Recent trends in robot learning and evolution for swarm robotics.

Swarm robotics is a promising approach to control large groups of robots. However, designing the individual behavior of the robots so that a desired collective behavior emerges is still a major challenge. In recent years, many advances in the automatic design of control software for robot swarms have been made, thus making automatic design a promising tool to address this challenge. In this article, I highlight and discuss recent advances and trends in offline robot evolution, embodied evolution, and offline robot learning for swarm robotics. For each approach, I describe recent design methods of interest, and commonly encountered challenges. In addition to the review, I provide a perspective on recent trends and discuss how they might influence future research to help address the remaining challenges of designing robot swarms.

Modular design of centrifugal microfluidic system and its application in nucleic acid screening.

Modular integration of functional components on the chip and increasement in control accuracy through real-time alteration in the force direction of droplets is an effective way to optimize centrifugal microfluidic systems and realize passive components, compact modules, and high-throughput control. Conventional centrifugal microfluidic chips are mainly driven and controlled by centrifugal force and Euler force. The control valves are easily affected by machining precision, making the control unstable. In this study, a novel centrifugal microfluidic system is introduced to improve the freedom and accuracy of chip control while facilitating the design and addition of passive functional components. Furthermore, we modularize the centrifugal microfluidic chip to greatly shorten the period of design and optimization cycle and achieve chip reusability and multi-threaded control. Finally, to verify the feasibility of the modular centrifugal microfluidic chip applied to high-throughput nucleic acid screening, we test the nucleic acid purification and detection colorimetric reactions based on the modular centrifugal microfluidic chip. Among them, Chelex-100 is used to realize the purification of nucleic acid in cell lysate, and the purified solution can realize amplification in the PCR instrument, and the nucleic acid detection results are consistent with the off-chip kit by experimental testing. The system has great flexibility and stability under the acceptable purity of nucleic acid, which indicates that the platform has great potential for large-scale rapid screening applications.

Development of mining workwear with high ergonomic performance fabrics based on the modular design concept.

Workwear is urgently needed in mining that is applied to ensure the basic safety and performance of miners. However, the protective and ergonomic functions of workwear are limited. This study aimed to develop and evaluate a new design of mining workwear. According to the literature review and survey research, the problems of current workwear were identified. Afterwards, the design specification was determined based on the modular design concept, including basic modules of typical styles and colors, functional modules, load-carrying modules and visibility straps. Appropriate materials were also selected to make the prototype. The results show that the new workwear was able to provide adequate thermal protection and decent ergonomic performance. Design of a reflective strap significantly improved the conspicuousness of the clothing. The mining workwear designed in this study has achieved good protection and wearing comfort. The modular design method has been proved effective in functional clothing design.

A Multifunctional 3D Bioprinting System for Construction of Complex Tissue Structure Scaffolds: Design and Application.

Three-dimensional (3D) bioprinting offers a potentially powerful new approach to reverse engineering human pathophysiology to address the problem of developing more biomimetic experimental systems. Human tissues and organs are multiscale and multi-material structures. The greatest challenge for organ printing is the complexity of the structural elements, from the shape of the macroscopic structure to the details of the nanostructure. A highly bionic tissue-organ model requires the use of multiple printing processes. Some printers with multiple nozzles and multiple processes are currently reported. However, the bulk volume, which is inconvenient to move, and the high cost of these printing systems limits the expansion of their applications. Scientists urgently need a multifunctional miniaturized 3D bioprinter. In this study, a portable multifunctional 3D bioprinting system was built based on a modular design and a custom written operating application. Using this platform, constructs with detailed surface structures, hollow structures, and multiscale complex tissue analogs were successfully printed using commercial polymers and a series of hydrogel-based inks. With further development, this portable, modular, low-cost, and easy-to-use Bluetooth-enabled 3D printer promises exciting opportunities for resource-constrained application scenarios, not only in biomedical engineering but also in the education field, and may be used in space experiments.

Toward an international platform: A web-based multi-language system for remote and virtual laboratories using react framework.

Universality is crucial for systems to provide services to users with different backgrounds. A good language system can remove language barriers to help to improve system universality. To improve user experience and provide new features to users, the Networked Control System Laboratory (NCSLab) has been redeveloped based on React. To achieve toward an international platform, this paper introduces a web-based multi-language system for remote and virtual laboratories based on React. The language system is implemented based on an open source tool named react-intl-universal. The architecture and modular design concept of the language system are investigated and the design and implementation are explored in detail from the perspective of the front-end and back-end separation scheme. The proposed language system has been integrated into the new React-based NCSLab system, which is scalable and can improve universality to serve not only domestic users in China, but also international users from all over the world.

Enhancement of nitrogen removal by a modular design of vertical flow constructed wetlands with a plant carbon source: Optimization of carbon dosage for nitrogen removal, practicability evaluation and strategy exploration for water quality control.

The requirement of artificial aeration for increasing nitrogen removal in vertical flow constructed wetlands (VFCWs) brings extra energy consumption and complex maintenance. The feasibility of a modular design to replace artificial aeration for partially saturated VFCWs with palm bark as a carbon source (PSVFCW-pb) to achieve water quality control, especially nitrogen removal was evaluated. The PSVFCW-pb with a spatially separate structure and perforated peripheries for better oxygen diffusion had a promising total nitrogen removal (e.g., 66.4% at a dosage of 1.435 g/L of palm bark pretreated at 120 °C for 40 min) without additional aeration, while organic carbon removal was nearly unaffected. An appropriate increase of the palm bark dosage (≤1.435 g/L) resulted in higher nitrogen removal; however, a more palm bark (1.875 g/L) could not further increase nitrogen removal but caused color pollution. In addition, the removal of nitrogen by the modularized PSVFCW-pb was more sensitive to the ambient temperature than the removal of organic carbon and phosphorus, and the higher temperature was preferable. Notably, the more attractive property of the modular design is its great potential to improve nitrogen removal by conveniently altering the number and/or scale of oxic and oxygen-free modules. Finally, the relationships between the hydraulic load and inflow concentration were explored, by which the suitable hydraulic load could be flexibly adjusted based on real-time water quality to meet the specified surface water quality criteria in different seasons. This study provides a reliable CW design for controlling nutrient pollution in surface waters.

Proteome reallocation enables the selective de novo biosynthesis of non-linear, branched-chain acetate esters.

The one-carbon recursive ketoacid elongation pathway is responsible for making various branched-chain amino acids, aldehydes, alcohols, ketoacids, and acetate esters in living cells. Controlling selective microbial biosynthesis of these target molecules at high efficiency is challenging due to enzyme promiscuity, regulation, and metabolic burden. In this study, we present a systematic modular design approach to control proteome reallocation for selective microbial biosynthesis of branched-chain acetate esters. Through pathway modularization, we partitioned the branched-chain ester pathways into four submodules including ketoisovalerate submodule for converting pyruvate to ketoisovalerate, ketoacid elongation submodule for producing longer carbon-chain ketoacids, ketoacid decarboxylase submodule for converting ketoacids to alcohols, and alcohol acyltransferase submodule for producing branched-chain acetate esters by condensing alcohols and acetyl-CoA. By systematic manipulation of pathway gene replication and transcription, enzyme specificity of the first committed steps of these submodules, and downstream competing pathways, we demonstrated selective microbial production of isoamyl acetate over isobutyl acetate. We found that the optimized isoamyl acetate pathway globally redistributed the amino acid fractions in the proteomes and required up to 23-31% proteome reallocation at the expense of other cellular resources, such as those required to generate precursor metabolites and energy for growth and amino acid biosynthesis. From glucose fed-batch fermentation, the engineered strains produced isoamyl acetate up to a titer of 8.8 g/L (>0.25 g/L toxicity limit), a yield of 0.22 g/g (61% of maximal theoretical value), and 86% selectivity, achieving the highest titers, yields and selectivity of isoamyl acetate reported to date.

Development of a Modular Reinforced Bone Tissue Engineering Scaffold with Enhanced Mechanical Properties.

A modular design composed of 3D-printed polycaprolactone (PCL) as the load-bearing module, and dual porosity gelatin foam as the bio-reactive module, was developed and characterized in this study. Surface treatment of the PCL module through aminolysis-aldehyde process was found to yield a stronger interface bonding compared to NaOH hydrolysis, and therefore was used in the fabrication procedure. The modular scaffold was shown to significantly improve the mechanical properties of the gelatin foam. Both compressive modulus and ultimate strength was found to increase over 10 times when the modular design was employed. The bio-reactive module i.e., gelatin foam, presented a dual porosity network of 100-300 µm primary and <10 µm secondary pores. SEM images revealed excellent attachment of DPSCs to the bio-reactive module.