VdP5CDH is involved in melanin formation, stress resistance and play a regulatory role in virulence of Verticillium dahliae.

Introduction: Verticillium dahliae, a soil-borne fungal pathogen, can cause cotton Verticillium wilt. In this study, VdP5CDH, the member of the ALDH_F4-17 family of carboxylate dehydrogenases, was identified in the genome of V. dahliae and investigated function in regulating virulence by generating gene deletion mutants and complementary mutants. Methods: Homologous recombination method was used to construct mutants, transcriptome sequencing revealed gene-related metabolic pathways, and disease degree of cotton was observed through pathogen infection experiments. Results: The conidial surface of VdP5CDH deletion strains was dented and shriveled, and the number of conidial spores increased. Compared with the wild-type (WT), the mycelial diameter of deletion mutants increased by 10.59%-11.16%, the mycelial growth showed irregular branching patterns, and misaligned arrangement. Although capable of penetrating cellophane, deletion mutants were unable to produce melanin. VdP5CDH was mainly associated with glucose metabolism, nitrogen metabolism, ABC transporter activity as well as various amino acid metabolic processes. After gene knockout, raffinose and pectin were used as the main carbon sources to promote the growth of strains and the growth rate of deletion strains in the medium containing raffinose was higher than that of WT. Consequently, the deletion mutant strains decreased utilization efficiency with which they utilized various nitrogen sources. The deletion mutants maintain responsiveness to osmotic stress and oxidative stress stimuli. Additionally, compared to WT strains, the deletion mutant strains exhibited differences in culture temperature tolerance, UV exposure response, and fungicide sensitivity. After cotton was infected with deletion strains conidial suspension, its disease index increased dramatically, while it gradually decreased after spraying with 2 mM glutamate in batches. With the increase of spraying times, the effect was more significant, and the disease index decreased by 18.95%-19.66% at 26 dpi. Discussion: These results indicated that VdP5CDH regulates the pathogenicity of fungi and controls mycelia growth, melanin formation, conidia morphology, abiotic stress resistance, and the expression of infecting structure-related genes.

Effect of increasing ß-mannanase supplementation in diets containing copra meal on growth performance, meat quality, liver health, intestinal morphology, and nutrient utilization in broiler chickens.

Objective: The current study aimed to investigate the effect of increasing ß-mannanase supplementation in diets containing copra meal (CM) on growth performance, meat quality, liver health, intestinal morphology, and nutrient utilization in broiler chickens. Methods: A total of 1,600 3-d-old Ross 308 broiler chickens (initial body weight [BW] ± SD = 43.3 ± 1.08 g) were randomly allotted to 1 of 5 treatment groups with 8 replicates. One group was fed a corn-soybean meal-based diet (control). Other 4 diets were prepared by inclusion of 10% commercial CM in the control diet with 0, 400, 800, and 1,600 U ß-mannanase/kg. Experiments lasted for 32 d. Results: Birds fed the control diet had less (p=0.001) feed conversion ratio (FCR) than those fed diets containing 10% CM without ß-mannanase supplementation. Increasing supplementation of ß-mannanase in diets containing 10% CM had no linear and quadratic effects on body weight gain, feed intake, and FCR in broiler chickens. The control diet had greater (p<0.01) apparent total tract retention (ATTR) of DM, GE, and N as compared to the diets containing 10% CM without ß-mannanase supplementation; however, no differences in the ATTR of Ca and P were identified between 2 diets. There were no linear and quadratic effects of increasing supplementation of ß-mannanase on the ATTR of DM, GE, N, Ca, and P in broiler diets containing 10% CM. Both inclusion of 10% CM and increasing supplementation of ß-mannanase in broiler diets did not affect apparent ME (AME) and N-corrected ME (AMEn) values in treatment diets. Conclusion: The use of 10% CM in broiler diets during growing and finishing period impairs growth performance by decreasing energy and nutrient utilization in diets. Increasing ß-mannanase supplementation in diets containing 10% CM has no positive effects on performance, meat quality, liver health, intestinal morphology, and nutrient utilization in broiler chickens.

Achyranthes japonica extract as phytogenic feed additive enhanced nutrient digestibility and growth performance in broiler.

Achyranthes japonica extract (AJE) is derived from a medicinal plant Achyranthes japonica, known for its anti-inflammatory, antioxidant, and antimicrobial properties. AJE contains multiple bioactive compounds, including saponins, triterpenoids, phytoecdysteroids, 20-hydroxyecdysone, and inokosterone. The aim of this investigation was to examine the impact of AJE as a phytogenic feed additive on growth performance, nutrient digestibility, excreta microbial count, noxious gas emissions, breast meat quality in broilers. About three hundred and sixty, day-old broilers (Ross 308) were assigned into four treatments (five replication cages/treatment, and 18 birds/cage). Dietary treatments: CON, basal diet; 0.02% AJE, basal diet with 0.02%; 0.04% AJE, basal diet with 0.04% AJE, and 0.06% AJE, basal diet with 0.06% of AJE. Body weight gain increased linearly (p < 0.05) through the inclusion of AJE during days 7 to 21, 21 to 35, as well as the entire experimental period. Besides, feed intake increased (p < 0.05) linearly during days 21 to 35 and the entire experiment with the increased AJE doses in broiler diet. Dry matter digestibility was increased (p < 0.05) linearly along with increasing amounts of AJE. With increasing AJE supplementation, nitrogen and energy utilization tended to improve (p < 0.10). In summary, the addition of AJE in the corn-soybean meal diet led to higher body weight gain and increased feed intake as well as enhanced nutrient digestibility, among them the highest improvement was found in 0.06%-AJE indicating the acceptance of AJE as a phytogenic feed additive.

Increasing Hybrid Rice Yield, Water Productivity, and Nitrogen Use Efficiency: Optimization Strategies for Irrigation and Fertilizer Management.

Water and fertilizer are crucial in rice growth, with irrigation and fertilizer management exhibiting synergies. In a two-year field study conducted in Yiyang City, Hunan Province, we examined the impact of three irrigation strategies-wet-shallow irrigation (W1), flooding irrigation (W2), and the "thin, shallow, wet, dry irrigation" method (W3)-in combination with distinct fertilizer treatments (labeled F1, F2, F3, and F4, with nitrogen application rates of 0, 180, 225, and 270 kg ha-1, respectively) on rice yield generation and water-fertilizer utilization patterns. The study employed Hybrid Rice Xin Xiang Liang you 1751 (XXLY1751) and Yue Liang you Mei Xiang Xin Zhan (YLYMXXZ) as representative rice cultivars. Key findings from the research include water, fertilizer, variety, and year treatments, which all significantly influenced the yield components of rice. Compared to W2, W1 in 2022 reduced the amount of irrigation water by 35.2%, resulting in a 42.0~42.8% increase in irrigation water productivity and a 25.7~25.9% increase in total water productivity. In 2023, similar improvements were seen. Specifically, compared with other treatments, the W1F3 treatment increased nitrogen uptake and harvest index by 1.4-7.7% and 5.9-7.7%, respectively. Phosphorus and potassium uptake also improved. The W1 treatment enhanced the uptake, accumulation, and translocation of nitrogen, phosphorus, and potassium nutrients throughout the rice growth cycle, increasing nutrient levels in the grains. When paired with the F3 fertilization approach, W1 treatment boosted yields and improved nutrient use efficiency. Consequently, combining W1 and F3 treatment emerged as this study's optimal water-fertilizer management approach. By harnessing the combined effects of water and fertilizer management, we can ensure efficient resource utilization and maximize the productive potential of rice.

Gut Microbiota Affects Host Fitness of Fall Armyworm Feeding on Different Food Types.

The fall armyworm (FAW), Spodoptera frugiperda, seriously threatens food and cash crops. Maize, wheat, and even rice damage by FAWs have been reported in many areas of China. It is urgent to clarify the mechanism which FAWs adapt to different feeding hosts and develop effective control technologies. Two-sex life tables and 16s rDNA sequencing were used to determine the host fitness and gut microbial diversity of FAWs when fed four different food types. Considering the life history parameters, pupa weight, and nutrient utilization indexes, the host fitness of FAWs when fed different food types changed in descending order as follows: artificial diet, maize, wheat, and rice. The gut microbial composition and the diversity of FAWs when fed different food types were significantly different, and those changes were driven by low-abundant bacteria. The gut microbes of FAWs that were fed with maize had the highest diversity. The functions of the gut microbes with significant abundance differences were enriched in nutrient and vitamin metabolism and other pathways that were closely related to host adaptation. Furthermore, we identified five genera (Acinetobacter, Variovorax, Pseudomonas, Bacillus, and Serratia) and one genus (Rahnella) that were positively and negatively correlated with the host fitness, respectively. This study revealed the possible role of gut microbes in the host adaptation of FAWs.

Dietary Replacement of Soybean Meal with Zanthoxylum bungeanum Seed Meal on Growth Performance, Blood Parameters, and Nutrient Utilization in Broiler Chickens.

Zanthoxylum bungeanum seed meal (ZBM), a novel plant protein raw material, has shown promising potential in enhancing the growth of broiler chickens as a substitute for soybean meal (SBM) in feed. In the artificial digestive experiment of vitro experiments, the digestibility of ZBM and SBM were assessed using the SDS-III Single Stomach Animal Biometric Digestion System. Subsequently, 180 1-day old AA chicks were divided into three groups for in vivo experiments: corn-soybean-meal-based diet (CON group); ZBM replacing 5% soybean meal in the basal diet (ZBM-1 group); ZBM replacing 10% soybean meal in the basal diet (ZBM-2 group). The experiment period lasted for 42 days. Compared to SBM, ZBM demonstrated higher crude protein content, dry matter digestibility, and extracorporeal digestible protein. Compared with the CON group, the broilers in the ZBM-2 group showed improved ADG and ADFI during the 1-21 d, 22-42 d, and 1-42 d periods (p < 0.05). Furthermore, the ZBM groups exhibited significant increases in slaughter performance compared with the CON group (p < 0.05). The substitution of ZBM for SBM also leads to a significant reduction in serum enzyme indicators (p < 0.05). Additionally, the lipoprotein and total cholesterol of the ZBM groups were significantly lower than those of the CON group (p < 0.05). Substituting SBM with ZBM significantly enhances the activity of superoxide dismutase and the content of immunoglobulin G in broiler serum, while reducing the content of malondildehyde (p < 0.05). The ZBM groups showed significantly higher utilization of dry matter, crude protein, and energy compared with the CON group (p < 0.05). In conclusion, the study confirmed that the substitution of SBM with 5-10% ZBM in broiler diets has a significant positive effect on growth, development, antioxidant capacity, immune function, and nutrient utilization. This study not only provides a theoretical foundation for the utilization of ZBM in broiler diets but also offers an effective approach for reducing reliance on soybean meal.

Effects of peppermint essential oil and artifier on growth performance, carcass characteristics and nutrient digestibilities in broiler chickens fed with low energy diets.

The aim of this experiment was to investigate the effects of peppermint essential oil (PEO) and an emulsifier called artifier on growth performance, carcass characteristics, and nutrient digestibility of broiler chickens fed low-energy diets. A total of 240 one-day-old male Ross 308 broiler chickens were divided into five treatments: control, low-energy diet, low-energy diet + 150 ppm PEO, low-energy diet + 300 ppm artifier, and low-energy diet + 150 ppm PEO + 300 ppm artifier. Each treatment was replicated four times in a completely randomized design. The chickens were fed these treatments during the starter, grower, and finisher periods for a total of 42 days. The results indicated that broiler chickens receiving the low-energy diet supplemented with PEO + artifier had similar body weight gain, feed conversion ratio, and breast percentage, but exhibited significantly lower abdominal fat percentage compared to those fed the control diet (P < 0.05). Furthermore, birds receiving PEO and artifier in low-energy diets, either individually or in combination, demonstrated higher digestibility of dry matter and fat during the starter and grower periods in comparison to those receiving the low-energy diet without any additives (P < 0.05). Over the entire rearing period, the simultaneous inclusion of 150 ppm PEO and 300 ppm artifier in the low-energy diet resulted in comparable growth performance to the control diet. Additionally, the concurrent use of PEO and artifier in the low-energy diet for broiler chickens led to a decrease in abdominal fat, increased digestibility of fat and dry matter, and enhanced nutrient utilization compared to the control diet.

Effects of dietary forage neutral detergent fiber and rumen degradable starch ratios on chewing activity, ruminal fermentation, ruminal microbes and nutrient digestibility of Hu sheep fed a pelleted total mixed ration.

Pelleted total mixed ration (P-TMR) feeding, which has become a common practice in providing nutrition for fattening sheep, requires careful consideration of the balance between forage neutral detergent fiber (FNDF) and rumen degradable starch (RDS) to maintain proper rumen functions. The present study aimed to investigate the effects of the dietary FNDF/RDS ratio (FRR) on chewing activity, ruminal fermentation, ruminal microbes, and nutrient digestibility in Hu sheep fed a P-TMR diet. This study utilized eight ruminally cannulated male Hu sheep, following a 4 × 4 Latin square design with 31 d each period. Diets consisted of four FRR levels: 1.0 (high FNDF/RDS ratio, HFRR), 0.8 (middle high FNDF/RDS ratio, MHFRR), 0.6 (middle low FNDF/RDS ratio, MLFRR), and 0.4 (low FNDF/RDS ratio, LFRR). Reducing the dietary FRR levels resulted in a linear decrease in ruminal minimum pH and mean pH, while linearly increasing the duration and area of pH below 5.8 and 5.6, as well as the acidosis index. Sheep in the HFRR and MHFRR groups did not experience subacute ruminal acidosis (SARA), whereas sheep in another two groups did. The concentration of total volatile fatty acid and the molar ratios of propionate and valerate, as well as the concentrate of lactate in the rumen linearly increased with reducing dietary FRR, while the molar ratio of acetate and acetate to propionate ratio linearly decreased. The degradability of NDF and ADF for alfalfa hay has a quadratic response with reducing the dietary FRR. The apparent digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber linearly decreased when the dietary FRR was reduced. In addition, reducing the dietary FRR caused a linear decrease in OTUs, Chao1, and Ace index of ruminal microflora. Reducing FRR in the diet increased the percentage of reads assigned as Firmicutes, but it decreased the percentage of reads assigned as Bacteroidetes in the rumen. At genus level, the percentage of reads assigned as Prevotella, Ruminococcus, Succinivibrio, and Butyrivibrio linearly decreased when the dietary FRR was reduced. The results of this study demonstrate that the dietary FRR of 0.8 is crucial in preventing the onset of SARA and promotes an enhanced richness of ruminal microbes and also improves fiber digestibility, which is a recommended dietary FRR reference when formulating P-TMR diets for sheep.

Forage neutral detergent fiber (FNDF) and rumen degradable starch (RDS) are key components of carbohydrates in the diet for ruminants, which would reflect saliva secretion and the acid production potential of feed. However, appropriate FNDF to RDS ratios (FRR) applicable to ruminants under the condition of pelleted total mixed ration (P-TMR) feeding have not been reported. In this study, we investigated the effects of the dietary FRR on chewing activity, ruminal fermentation, ruminal microbial communities, and nutrient digestibility of Hu sheep under P-TMR feeding. The results indicate that reducing dietary FRR levels would induce acidosis in sheep, which negatively affected fiber utilization and ruminal bacterial communities. The FRR of 0.8 was a recommended dietary FRR when formulating a P-TMR diet for fattening sheep, as indicated by decreased ruminal acidosis risk and increased richness of ruminal microbes in the rumen as well as nutrient digestibility.

The Substitution of Fishmeal with Yeast Culture in the Yellow Catfish (Pelteobagrus fulvidraco) Diet: Growth, Serum Biochemical Indices, and Intestinal and Hepatopancreatic Histology.

Yeast culture is a complex fermentation product consisting of fermentation substrate, yeast cells and their metabolites. This study investigated the potential of yeast culture in replacing fishmeal in the diet of yellow catfish (Pelteobagrus fulvidraco). First, a basal diet was formulated to contain 160 g/kg fishmeal (CON), and then the dietary fishmeal was decreased to 120, 80, 40 and 0 g/kg via yeast culture inclusion, respectively, to form another four isonitrogenous and isolipidic diets (YC-12, YC-8, YC-4 and YC-0). Yellow catfish (3.00 ± 0.10 g) were fed with the above five diets with triplicates per treatment and 40 fish per replicate. After 8 weeks of feeding, the weight gain (WG), protein efficiency rate and protein retention in the YC-12 group and the feed conversion ratio (FCR) in the YC-12 and YC-8 groups showed no significant differences to the CON group (p > 0.05), but the WG in the YC-8, YC-4 and YC-0 groups was significantly lower, and the FCR in the YC-4 and YC-0 groups was significantly higher than in the CON group (p < 0.05). In terms of the whole-body composition, only the crude lipid content in the YC-0 group decreased significantly (p < 0.05). Compared with the CON group, the aspartate aminotransferase and alanine aminotransferase activities and D-lactic acid content in the YC-0 group were significantly increased, and the total cholesterol content was significantly reduced (p < 0.05). The activities of catalase, superoxide dismutase, and alkaline phosphatase, as well as the content of complement C3 and immunoglobulin M, were significantly increased, while the MDA content was significantly reduced in the YC-12 and YC-8 groups (p < 0.05). There were no significant differences in the intestinal amylase and lipase activity among all the groups (p > 0.05), while the trypsin activity in the YC-12 and YC-8 groups, as well as the diamine oxidase in the YC-4 and YC-0 groups, were significantly higher than those in the CON group (p < 0.05). In the intestine histology, there was a significant decrease in the intestinal villus height in the YC-4 and YC-0 groups as well as in the villus width in the YC-0 group (p < 0.05). In the hepatopancreas histology, lipid droplets appeared in the YC-4 and YC-0 groups, and severe cell vacuolation was observed in the YC-0 group. As a summary, in a practical diet containing 160 g/kg fishmeal, yeast culture can effectively replace 40 g/kg fishmeal without negatively affecting the growth performance, nutrient utilization, serum immune and antioxidant, intestinal and hepatopancreas histology of yellow catfish.

Environmental impacts on algal-bacterial-based aquaponics system by different types of carbon source addition: water quality and greenhouse gas emission.

Carbon source addition is an important way improving the carbon and nitrogen transformation in aquaculture system; however, its effectiveness of algal-bacterial-based aquaponics (AA) through carbon source addition is still vague. In this study, the influences of organic carbon (OC-AA system) and inorganic carbon (IC-AA system) addition and without carbon source addition (C-AA system) on the operational performance of AA system were investigated. Results showed that 10.1-19.5% increase of algal-bacterial biomass enhanced the purifying effect of ammonia nitrogen in OC-AA system and IC-AA system relative to C-AA system. Moreover, extra electron donor supply in the OC-AA system obtained the lowest NO3--N concentration. However, that was at the cost of aggravated N2O conversion ratio, which increased by more than 2.0-folds than other systems, attributing to 2.9-folds increase of nirS gene abundance. In addition, carbon source addition increased the pH and then decreased the fish biomass production of AA system. The results of this study would provide theoretical supports of carbon source addition on the performance of nutrient transformation and greenhouse gas effect in AA system.

Effects of the concentration and nature of total dissolved solids in drinking water on feed intake, nutrient digestion, energy balance, methane emission, ruminal fermentation, and blood constituents in different breeds of young goats and hair sheep.

Understanding how different livestock species and breeds respond to consumption of brackish water could improve usage of this resource. Therefore, Angora, Boer, and Spanish goat doelings and Dorper, Katahdin, and St. Croix ewe lambs (6 animals per animal type [AT]; initial age = 296 ± 2.1 days) consuming water with varying concentrations of minerals of a natural brackish water source (BR) and sodium chloride (NaCl; SL) were used to determine effects on water and feed intake, nutrient digestion, heat energy, methane emission, ruminal fluid conditions, and blood constituent concentrations. There were 6 simultaneous 6 (water treatments [WT]) × 6 (AT) Latin squares with 3-wk periods. The WT were fresh (FR), BR alone (100-BR), a similar total dissolved solids (TDS) concentration as 100-BR via NaCl addition to FR (100-SL), BR with concentrations of all minerals increased by approximately 50% (150-BR), a similar TDS level as 150-BR by NaCl addition to FR (150-SL), and a similar 150 TDS level achieved by addition of a 1:1 mixture of BR minerals and NaCl to 100-BR (150-BR/SL). Concentrations (mg/kg) in BR were 4928 TDS, 85.9 bicarbonate, 224.9 calcium, 1175 chloride, 60.5 magnesium, 4.59 potassium, 1387 sodium, 1962 sulfate, and 8.3 boron, and TDS in other WT were 209, 5684, 7508, 8309, and 7319 mg/kg for FR, 100-SL, 150-BR, 150-SL, and 150-BR/SL, respectively. There were very few significant effects of WT or AT × WT interactions, although AT had numerous effects. Water intake was affected by AT (P = 0.02) and WT (P = 0.04), with greater water intake for 150-SL than for FR, 100-BR, 100-SL, and 150-BR. Dry matter intake among AT was lowest (P < 0.05) for Angora. Digestion of organic matter and neutral detergent fiber and heat energy differed among AT (P < 0.05), but nitrogen digestion and ruminal methane emission were similar among AT. Blood aldosterone concentration was higher (P < 0.05) for FR than for other WT. In conclusion, all AT seemed resilient to these WT regardless of mineral source and concentrations, with TDS less than 8300 mg/kg, which did not influence nutrient utilization, ruminal fermentation, energy balance, or blood constituent levels.

Mycorrhizal mediation of soil carbon in permafrost regions depends on soil nutrient stoichiometry and physical protection.

Mycorrhizal associations are considered as one of the key drivers for soil carbon (C) accumulation and stability. However, how mycorrhizal associations influence soil organic C (SOC) and its fractions (i.e., particulate organic C [POC] and mineral-associated organic C [MAOC]) remain unclear. In this study, we examined effects of plant mycorrhizal associations with arbuscular mycorrhiza (AM), ectomycorrhiza (ECM), and their mixture (Mixed) on SOC and its fractions as well as soil stoichiometric ratios across 800-km transect in permafrost regions. Our results showed that soil with only ECM-associated trees had significantly higher SOC and POC compared to only AM-associated tree species, while soil in Mixed plots with both AM- and ECM- associated trees tend to be somewhat in the middle. Using structural equation models, we found that mycorrhizal association significantly influenced SOC and its fraction (i.e., POC, MAOC) indirectly through soil stoichiometric ratios (C:N, C:P, and N:P). These results suggest that selecting ECM tree species, characterized by a "slow cycling" nutrient uptake strategy, can effectively enhance accumulation of SOC and its fractions in permafrost forest ecosystems. Our findings provide novel insights for quantitatively assessing the influence of mycorrhiza-associated tree species on the management of soil C pool and biogeochemical cycling.

Impact of dietary digestible aromatic amino acid levels and stachyose on growth, nutrient utilization, and cecal odorous compounds in broiler chickens.

This study evaluated the impact of dietary digestible aromatic amino acid (DAAA) levels and stachyose on growth, nutrient utilization and cecal odorous compounds in broiler chickens. A 3×2 two-factor factorial design: Three dietary DAAA levels (1.40, 1.54, 1.68%) supplemented with either 5 g/kg of stachyose or without any stachyose were used to create 6 experimental diets. Each diet was fed to 6 replicates of 10 birds from d 22 to 42. Findings revealed that broilers receiving a diet with 1.54% DAAA levels supplemented with 5 g/kg stachyose exhibited a significant boost in average daily gain and improved utilization of crude protein, ether extract, tryptophan, and methionine compared to other diet treatments (P < 0.05). As the dietary DAAA levels increased, there was a significant rise in the concentrations of indole, skatole, p-methylphenol, and butyric acid in the cecum of broilers (P < 0.05). The addition of stachyose to diets reduced concentrations of indole, skatole, phenol, p-methylphenol, acetic acid and propionic acid in the cecum (P < 0.05). The lowest concentrations of indole, phenol, p-methylphenol, volatile fatty acids and pH in cecum of broilers were observed in the treatment which diet DAAA level was 1.40% with stachyose (P < 0.05). In conclusion, dietary DAAA levels and stachyose had significant interactions on the growth, main nutrient utilization and cecal odorous compounds in broilers. The dietary DAAA level was 1.54% with 5 g/kg of stachyose can improve the growth performance, nutrient utilization. However, the dietary DAAA level was 1.40% with stachyose was more beneficial to decrease the cecal odor compound composition in broilers.

Enhancing the Production Performance and Nutrient Utilization of Laying Hens by Augmenting Energy, Phosphorous and Calcium Deficient Diets with Fungal Phytase (Trichoderma reesei) Supplementation.

A ten-week trial was conducted to evaluate the enhancement of production performance and nutrient utilization of laying hens through augmenting energy, phosphorous, and calcium deficient diets with fungal phytase (Trichoderma reesei) supplementation. 720 Hy-line Brown hens aged 28 weeks were randomly divided into 5 groups; each group had 8 replicates of 18 hens. Five experimental diets were prepared and fed to corresponding groups. A positive control (PC) diet contained 3.50% of calcium (Ca), 0.32% of non-phytate phosphorus (NPP), and apparent metabolic energy (AME) of 11.29MJ/kg, while a negative control (NC) diet contained 3.30% of Ca, 0.12% of NPP, and lower AME of 300 kJ/kg. The other three diets were supplemented with 250 FTU/kg phytase (PHY-250), 1000 FTU/kg phytase (PHY-1000), and 2000 FTU/kg phytase (PHY-2000) in addition to a regular NC diet. Results indicated that the positive control (PC) diet group had higher body weight gain, egg weight, and average daily feed intake. However, laying rate, egg mass, and FCR were most improved in the PHY-2000 group, followed by the PHY-1000 and PHY-250 groups (p < 0.05). Improved yolk color was most notable in laying hens fed the diet with PHY-1000 as opposed to the PC and NC groups (p < 0.05), but no overall difference was found among all of the phytase treated groups. The apparent availability of dry matter, energy, phosphorus, and phytate P was significantly higher in the PHY-2000 group than in the PC and NC groups (p < 0.05). Compared to the PC group, nitrogen retention was significantly higher in the PHY-1000 group, while calcium availability was higher in the PHY-250 group. The results suggested that the addition of phytase to diets with low P, Ca, and AME improved laying performance and apparent availability of dietary nutrients. Thus, it was concluded that the laying hen diet could be supplemented with 1000-2000 FTU/kg phytase for improving laying production and nutrient availability and mitigating the negative impact of reduced nutrient density in laying hen diets.

Dietary sodium butyrate positively modulated intestinal microbial community, but did not promote growth of largemouth bass (Micropterus salmoides).

This study investigated the effects of dietary sodium butyrate (NaB) on growth, serum biochemical indices, intestine histology, and gut microbiota of largemouth bass (Micropterus salmoides). A basal diet was formulated and used as the control diet (Con), and five additional diets were prepared by supplementing NaB (50%) in the basal diet at 2.0, 4.0, 8.0, 12.0, and 16.0 g/kg inclusion (NaB-2, NaB-4, NaB-8, NaB-12, and NaB-16 diets). Then, the six diets were fed to triplicate groups of largemouth bass juveniles (2.4 ± 0.1 g) for 8 weeks. NaB supplementation linearly and quadratically affected weight gain (WG) and feed intake (FI) (P < 0.05). The NaB-16 group displayed lower WG (- 6.8%) and FI than the Con group (P < 0.05), while no differences were found in WG and feed conversion ratio between the other NaB groups and Con group (P > 0.05). Serum alkaline phosphatase and lysozyme activities were higher in the NaB groups (P < 0.05), and D-lactate content was lower in the NaB-12 group (P < 0.05) than the control. Intestinal lipase activity in NaB-2, NaB-4 group, and villi width in NaB-8 group were also higher than those in the Con group (P < 0.05). Compared to the Con group, the intestinal abundances of Firmicutes and Mycoplasma were increased and the abundances of Proteobacteria, Achromobacter and Plesiomonas were decreased in NaB-4 and NaB-16 groups (P < 0.05). In conclusion, dietary NaB did not promote the growth of juvenile largemouth bass, but positively modulated the intestinal microbial community.

Effects of Dietary Fiber Level and Forage Particle Size on Growth, Nutrient Digestion, Ruminal Fermentation, and Behavior of Weaned Holstein Calves under Heat Stress.

This experiment was designed to investigate the effects of feeding diets with different fiber content and forage particle size on the performance, health, nutrient digestion, rumen fermentation, and behavioral and sorting activity of Holstein dairy calves kept under elevated environmental temperature. Sixty weaned Holstein female calves (age = 96.7 ± 7.62 days old; body weight = 82.4 ± 10.4 kg) were randomly assigned to one of 4 treatments arranged in a 2-by-2 factorial design in a 70-day experiment. Dietary forage content (moderate, 22.5%; or high, 40.0% on DM basis) and alfalfa hay particle size (short, 4.39 mm; or long, 7.22 mm as geometric mean) were the experimental factors, resulting in the following combinations: (1) high-fiber (HF) diets with forage-to-concentrate ratio of 40:60 and long particle-sized alfalfa hay (LPS; HF-LPS); (2) HF diets with short particle-sized alfalfa hay (SPS; HF-SPS); (3) moderate-fiber (MF) diets with forage-to-concentrate ratio of 22.5:77.5 with LPS (MF-LPS); and (4) MF diets with SPS (MF-SPS). The temperature-humidity index averaged 73.0 ± 1.86, indicating that weaned calves experienced a moderate extent of heat stress. Fiber level and AH particle size interacted and affected dry matter intake, with the greatest intake (4.83 kg/d) observed in MF-SPS-fed calves. Final body weight was greater in calves receiving MF vs. HF diets (164 vs. 152 kg; p < 0.01). Respiration rate decreased when SPS vs. LPS AH was included in HF but not MF diet. Lower rectal temperature was recorded in calves fed MF vs. HF diet. Digestibility of dry matter and crude protein was greater in calves fed MF than HF diets, resulting in lower ruminal pH (6.12 vs. 6.30; p = 0.03). Fiber digestibility was greater in calves fed SPS compared with those fed LPS alfalfa hay. Feeding HF compared with MF diet increased acetate but lowered propionate molar proportions. The inclusion of SPS vs. LPS alfalfa hay decreased lying time in HF diet (920 vs. 861 min; p < 0.01). Calves fed MF vs. HF diets spent less time eating but more time lying, which is likely indicative of better animal comfort. Dietary fiber level and forage particle size interacted and affected sorting against 19 mm particles, the extent of which was greater in HF-SPS diet. Overall, dietary fiber level had a stronger effect than forage particle size on the performance of weaned calves exposed to a moderate degree of heat stress as feeding MF vs. HF diet resulted in greater feed intake, final body weight, structural growth measures, nutrient digestion, as well as longer lying behavior. The inclusion of SPS alfalfa hay in MF diets increased feed consumption.

Distinctive plastome evolution in carnivorous angiosperms.

BACKGROUND: Independent origins of carnivory in multiple angiosperm families are fabulous examples of convergent evolution using a diverse array of life forms and habitats. Previous studies have indicated that carnivorous plants have distinct evolutionary trajectories of plastid genome (plastome) compared to their non-carnivorous relatives, yet the extent and general characteristics remain elusive. RESULTS: We compared plastomes from 9 out of 13 carnivorous families and their non-carnivorous relatives to assess carnivory-associated evolutionary patterns. We identified inversions in all sampled Droseraceae species and four species of Utricularia, Pinguicula, Darlingtonia and Triphyophyllum. A few carnivores showed distinct shifts in inverted repeat boundaries and the overall repeat contents. Many ndh genes, along with some other genes, were independently lost in several carnivorous lineages. We detected significant substitution rate variations in most sampled carnivorous lineages. A significant overall substitution rate acceleration characterizes the two largest carnivorous lineages of Droseraceae and Lentibulariaceae. We also observe moderate substitution rates acceleration in many genes of Cephalotus follicularis, Roridula gorgonias, and Drosophyllum lusitanicum. However, only a few genes exhibit significant relaxed selection. CONCLUSION: Our results indicate that the carnivory of plants have different effects on plastome evolution across carnivorous lineages. The complex mechanism under carnivorous habitats may have resulted in distinctive plastome evolution with conserved plastome in the Brocchinia hechtioides to strongly reconfigured plastomes structures in Droseraceae. Organic carbon obtained from prey and the efficiency of utilizing prey-derived nutrients might constitute possible explanation.

Moringa oleifera leaf meal supplementation improves nutrient digestibility, milk yield, and reproductive performances in dairy cows during early lactation.

The study aimed to evaluate the effects of Moringa oleifera leaf meal (MOLM) supplementation on nutrient utilization, milk yield, and reproductive performance of early lactating Sahiwal cows. Control cows (GC) received a basal diet, while the treatment cows (GM) were supplemented with concentrate comprising 12% MOLM. Ovarian activity and uterine involution were monitored by trans-rectal ultrasonography on the 21st, 28th, 35th, and 42nd days postpartum. The result indicated that MOLM-supplemented cows required fewer days (P ≤ 0.05) to complete uterine involution. As lactation progresses, there was a significant reduction (P ≤ 0.05) in the diameter of the cervix and uterine horns in GM than GC. There was a significant increase in the number of follicles on the 21st day and average milk yield in GM than GC. The incidence of endometritis and cystic ovarian disease was less in MOLM supplemented group. The use of MOLM in the diet reduced the total cost per cow per successful service. It is concluded that MOLM can be safely included at 12% in the diet of early lactating cows to modulate the reproductive performances of dairy cows. Dairy farmers can use moringa leaf meal to feed their dairy cows, which is cheaper and improves production and reproduction performance.

Reduction of methane and nitrogen emission and improvement of feed efficiency, rumen fermentation, and milk production through strategic supplementation of eucalyptus (Eucalyptus citriodora) leaf meal in the diet of lactating buffalo (Bubalus bubalis).

Buffalo plays a compelling role in reducing malnutrition and ensuring food to the people of Asian countries by its major contribution to milk and meat pool of the livestock agriculture farming system in the region. As Asia is the home for more than 90% of world buffalo population, they are also one of the largest emitters of greenhouse gasses. Eucalyptus (Eucalyptus sp.) leaves are rich sources of naturally occurring essential oils and phenolic compounds, which could modulate rumen fermentation through mitigation of methanogenesis and nitrogen excretion along with stimulation of immune system and production performances of animals. Therefore, the present study investigated the impact of dietary inclusion of eucalyptus (Eucalyptus citriodora) leaf meal (ELM) on voluntary feed intake, rumen functions, methane emission, nutrient utilization, milk yield and fatty acids profile, and immune response in lactating buffalo (Bubalus bubalis). An in vitro experiment conducted with graded dose (10-40 g/kg) inclusion of ELM into the total mixed ration to select ideal level for feeding to lactating buffaloes, an improvement (P < 0.05) in feed degradability (IVDMD), microbial biomass and ruminal volatile fatty acids concentration with reduced (P < 0.05) methane and ammonia-N production were evidenced when ELM was added at 10-20 g/kg DM, beyond which negative effects on rumen fermentation were pronounced. An in vivo experimentation was conducted with sixteen Murrah (Bubalus bubalis) buffaloes of mean live weight, 544.23 ± 10.02 kg; parity, 2-4 at initial stage (~60 days) of lactation with average milk yield of 11.43 ± 1.32 kg and were divided into two groups (CON, ELM) of eight each in a completely randomized design. All the animals were kept individually on wheat straw-based diet with required quantity of concentrate mixture and green fodder. The control group buffaloes were fed a total mixed ration; however, the treatment group (ELM) was supplemented with 10 g/kg DM diet of dry grounded eucalyptus (Eucalyptus citriodora) leaves by mixing with the concentrate mixture. The feeding experiment was conducted for 120 days, including 15 days for adaptation to the experimental diets and 105 days for data recording. The nutrient digestibility (DM, OM, CP, and EE) was improved (P < 0.05) without affecting feed intake (P > 0.05) and fiber digestibility (NDF and ADF) in ELM supplemented buffaloes. Increased (P < 0.05) milk production and rumenic acid concentration (cis 9 trans 11 C18:2 CLA) were demonstrated with comparable (P > 0.05) milk composition and major fatty acids profile of milk in the supplemented buffaloes. Dietary inclusion of ELM reduced (P < 0.05) enteric methane production and fecal excretion of nitrogen. The health status of buffaloes fed ELM improved throughout the experimental period was improved by enhancing cell mediated (P = 0.09) and humoral (P < 0.01) immune responses without affecting (P > 0.05) major blood metabolites. The study described feeding ELM at 10 g/kg diet to lactating Murrah buffaloes as a natural source of phenols and essential oils to increase milk production and CLA content, reduce methane and nitrogen emissions, and improve health status. Thus, feeding of ELM could be beneficial for climate smart buffalo production system for enhancing milk production with lesser impact on environment.

Influence of Select Dietary Trace Minerals and their Levels on Growth Performance, Nutrient Utilization and Minerals Balance in Male Goats.

A study was conducted on eighteen male goats to evaluate the effect of incremental levels of select dietary trace minerals on growth performance, nutrient utilization and minerals balance. Animals (16.65 ± 0.53 kg average BW) were randomly divided into three groups and fed a basal diet to meet the requirement of all the nutrients except select trace minerals for 60 days. Groups were T1 (0.204 ppm Se, 35.29 ppm Zn, 7.37 ppm Cu, 39.79 ppm Mn), T2 (0.504 ppm Se, 55.29 ppm Zn, 17.37 ppm Cu, 59.79 ppm Mn), and T3 (0.804 ppm Se, 75.29 ppm Zn, 27.37 ppm Cu, 79.79 ppm Mn). Daily dry matter intake (DMI) (% BW) was lower (P < 0.05) in T3 as compared to other groups however, average daily gain (ADG) in body weight (P = 0.113) and feed: gain ratio (P = 0.102) showed subtle improvement in T2 and T3 groups. Feeding of higher levels of select trace minerals did not affect the intake and digestibility of nutrients, or plane of nutrition, however, the digestibility of non-fiber carbohydrates (NFC) increased (P = 0.004) in T2 and T3 groups than T1. The balance of N, Ca, P and Cu did not differ among the groups but balance of Zn, Mn and Se increased in T2 and T3 groups than T1 group. It is concluded that feeding higher levels of Zn (75.29 ppm), Cu (27.37 ppm), Mn (79.79 ppm) and Se (0.804 ppm) in combination, improved NFC digestibility, retention of Zn, Mn and Se and had subtle beneficial effects on the growth performance of male goats.