The Occurrence of a Negative Energy Balance in Holstein-Friesian and Simmental Cows and Its Association with the Time of Resumption of Reproductive Activity.

Intensive lactation (lactogenesis) in cows is conducive to a negative energy balance (NEB), so the search for traits associated with the physiological capacity to cope with its consequences is a current area of research. This is especially important because NEB overlaps with the resumption of the reproductive cycle, which determines the profitability of herds. This study analysed the relationship between NEB and the time of resumption of reproductive activity in cows with varying genetic potential (Simmental and Holstein-Friesian), fed a similar diet (TMR). The aim of the study was to analyse the dependencies between NEB markers and changes in progesterone levels between 25 and 31 days postpartum. A strong positive correlation was shown between daily milk production (DMP) and loss of body condition (LBCS; 0.772; p ≤ 0.05). These parameters were associated with the levels of NEB biomarkers. Higher values of NEB indicators (LBCS, C16:0, C18:1, NEFA, and BHBA) were usually noted during periods with higher DMP (II and III). The trends observed were confirmed by positive correlation coefficients (r), which ranged from 0.324 to 0.810 (p ≤ 0.05). The reverse trend was noted for glucose and leptin, which decreased as productivity increased, as confirmed by r values from -0.368 to -0.530 (p ≤ 0.05). In both breeds, the glucose and leptin levels decreased as DMP increased. Higher values for NEB indicators were shown to be negatively correlated with progesterone levels (r from -0.300 to -0.712; p ≤ 0.05), and a lower progesterone level was associated with a longer calving-to-first-service interval and calving-to-conception interval. The rate of postpartum triglyceride release depends on daily milk production, and therefore the adaptability of the liver should be considered an important element of mitigation of the consequences of NEB. This may have practical applications by extending productive life, which is often shortened due to deteriorating reproductive performance.

Effects of trans-2-hexenal on reproduction, growth and behaviour and efficacy against the pinewood nematode, Bursaphelenchus xylophilus.

BACKGROUND: Bursaphelenchus xylophilus is a serious quarantined pest that causes severe damage and major economic losses to pine forests. Because of the adverse effects of some traditional nematicides on humans and the environment, the search for new plant toxicants against these nematodes has intensified. Nematicidal activity of trans-2-hexenal, which is a six-carbon aldehyde present in many plants, was tested against the nematode. RESULTS: trans-2-Hexenal showed significant efficacy against B. xylophilus in a dose range of 349.5-699 g m-3 by fumigation of pinewood logs. Additionally, it had significant nematicidal activity against different life stages of B. xylophilus in an in vitro test, with second-stage larvae (L2s) being the most sensitive, with an LC50 value of 9.87 µg mL-1 at 48 h. Egg hatch was also significantly inhibited. Further studies revealed that trans-2-hexenal inhibited the reproductive activity of B. xylophilus, with negative effects on reproduction rate and egg numbers. Moreover, trans-2-hexenal reduced the body length of B. xylophilus. Respiratory rate and thrashing behaviour of B. xylophilus also decreased following treatment with this compound. CONCLUSION: trans-2-Hexenal had significant nematicidal activity against B. xylophilus, providing a basis for elucidation of the mode of action of trans-2-hexenal against plant-parasitic nematodes in future studies. © 2016 Society of Chemical Industry.

Effect of ß-carotene Supplementation on Italian Trotter Mare Peripartum.

When the mare's estrous cycle resumes in winter, the ß-carotene content of hay is depleted. Sixty Italian trotter mares were randomly assigned to a Control or a Treated Group. Treated Group received 1g/d synthetic ß-carotene for 15 days from parturition. Blood samples collected at parturition and on days 5, 10 and 15 after partum were analysed for ß-carotene, vitamins A, progesterone, 17 ß-estradiol, the energy parameters (glucose, cholesterol, NEFA), the protein profile (total protein, albumin, urea) and LDH. Some changes in these measures were attributable to treatment, which significantly affected ß-carotene and 17 ß-estradiol concentrations. A significant effect was also found on the resumption of estrous activity (χ(2) test=P<0.052).