The contribution of swine wastewater on environmental pathogens and antibiotic resistance genes: Antibiotic residues and beyond.

Swine wastewater application can introduce antibiotics, antibiotic resistance genes (ARGs) into environments. Herein, the full-scale transmission of antibiotics, ARGs and their potential carriers from an intensive swine feedlot to its surroundings were explored. Results showed that lincomycin and doxycycline hydrochloride were dominant antibiotics in this ecosystem. Lincomycin concentration were strongly associated with soil bacterial communities. According to the risk quotient (RQ), lincomycin was identified as posing higher ecological risk in aquatic environments. ARGs and mobile genetic elements (MGEs) abundance in wastewater were reduced after anaerobic treatment. Notably, ARGs composition of environmental samples were clustered into two groups based on if they were directly affected by the wastewater. However, there were no remarkable difference of ARGs abundance among environmental samples. The total abundance of ARGs was positively related to that of MGEs. Pathogens Escherichia coli and Enterococcus revealed strong connection with qnrS, tet and sul. Overall, this study highlights the importance of responsible antibiotics use in livestock production and appropriate treatment technology before agricultural application and discharge.

Comparative analysis of water quality index and river classification in Kereh River, Penang, Malaysia: Impact of untreated swine wastewater from Kampung Selamat pig farms.

The Kereh River in Penang, Malaysia, has faced severe pollution for over 40 years due to untreated wastewater from swine farms in Kampung Selamat, discharged via stormwater drains. Despite official claims that all 77 swine farms treat their wastewater to meet regulatory standards, local non-governmental organizations and villagers have challenged this, though their concerns lack scientific backing. This study evaluates the river's water quality by analyzing samples from upstream (US), midstream (MS), and downstream (DS), and from Parit Cina-Parit Besar, a conduit for untreated swine wastewater. Fourteen parameters were measured against Malaysia's National Water Quality Standards (NWQS). Significant differences were found in six parameters: ammonium nitrogen (AN), biochemical oxygen demand (BOD), chemical oxygen demand (COD), dissolved oxygen (DO), total suspended solids (TSS), and oil and grease (OG). While Dunn's post hoc pairwise comparison showed no significant differences among river segments, mean values indicated increased pollution downstream, particularly after the convergence with untreated swine wastewater. River classification worsened, with water quality index dropping from 69.88 ± 11.37 score (Class III) US to 38.49 ± 12.74 and 50.44 ± 3.14 scores (Class IV) MS and downstream, respectively. A significant positive correlation between E. coli and AN (r = 0.71, p < 0.01) suggests a common point source pollutant, particularly the untreated swine wastewater. The river exhibits low oxygen levels and high organic matter and nutrient concentrations, especially MS and downstream, highlighting substantial ecological and public health risks. Effective enforcement of waste treatment regulations and enhanced monitoring are crucial for mitigating pollution and restoring the river's ecosystem. Collaboration between authorities and pig farmers is essential to improve water quality and maintain the river's ecological balance. PRACTITIONER POINTS: Severe Kereh River pollution: Untreated swine wastewater from Kampung Selamat pig farms, primarily via Parit Cina-Parit Besar, has degraded the river for over 40 years. Regulatory non-compliance: Despite official claims, untreated swine wastewater continues to pollute the river, challenging regulatory standards. Significant pollution indicators: Elevated levels of AN, BOD, COD, DO, TSS, OG, and E. coli signal severe pollution midstream and downstream. Water quality index drop: WQI scores classify midstream and downstream sections as polluted, indicating worsening conditions downstream. Urgent need for action: Enforcing regulations, improving wastewater treatment, and relocating pig farms are crucial for restoring the Kereh River.

Aerobic granular sludge for swine wastewater treatment: Implications for antibiotic and antibiotic resistance gene elimination.

Swine wastewater (SW) contains high levels of traditional pollutants, antibiotics, and antibiotic resistance genes (ARGs), necessitating effective elimination. Two parallel aerobic granular sludge (AGS) reactors, R1 and R2, were constructed and optimized for treating SW from two pig farms, identified as SW1 and SW2. R2 showed higher antibiotic removal efficiency, particularly in the removal of sulfonamides, while fluoroquinolones tended to adsorb onto the sludge. Process optimization by introducing an additional anoxic phase enhanced denitrification and reduced effluent ARG levels, also aiding in the improved removal of fluoroquinolones. The nitrite-oxidizing bacteria (NOB) Nitrospira accumulated after the treatment process, reaching 12.8 % in R1 and 14.1 % in R2, respectively. Mantel's test revealed that pH, NH4+-N, and Mg significantly affected ARGs and microbial community. Sulfadiazine and sulfamethazine were found to significantly impact ARGs and the microbial communities. This study provides innovative insights into the application of AGS for the treatment of real SW.

Study on the biodegradation characteristics and mechanism of tetracycline by Serratia entomophila TC-1.

Microbial degradation is an important solution for antibiotic pollution in livestock and poultry farming wastes. This study reports the isolation and identification of the novel bacterial strain Serratia entomophila TC-1, which can degrade 87.8 % of 200 mg/L tetracycline (TC) at 35 °C, pH 6.0, and an inoculation amount of 1 % (v/v). Based on the intermediate products, a possible biological transformation pathway was proposed, including dehydration, oxidation ring opening, decarbonylation, and deamination. Using Escherichia coli and Bacillus subtilis as biological indicators, TC degraded metabolites have shown low toxicity. Whole-genome sequencing showed that the TC-1 strain contained tet (d) and tet (34), which resist TC through multiple mechanisms. In addition, upon TC exposure, TC-1 participated in catalytic and energy supply activities by regulating gene expression, thereby playing a role in TC detoxification. We found that TC-1 showed less interference with changes in the bacterial community in swine wastewater. Thus, TC-1 provided new insights into the mechanisms responsible for TC biodegradation and can be used for TC pollution treatment.

Performance of four different microalgae-based technologies in antibiotics removal under multiple concentrations of antibiotics and strigolactone analogue GR24 administration.

The formation of symbionts by using different combinations of endophytic bacteria, microalgae, and fungi to purify antibiotics-containing wastewater is an effective and promising biomaterial technology. As it enhances the mixed antibiotics removal performance of the bio-system, this technology is currently extensively studied. Using exogenous supplementation of various low concentrations of the phytohormone strigolactone analogue GR24, the removal of various antibiotics from simulated wastewater was examined. The performances of Chlorella vulgaris monoculture, activated sludge-C. vulgaris-Clonostachys rosea, Bacillus licheniformis-C. vulgaris-C. rosea, and endophytic bacteria (S395-2)-C. vulgaris-C. rosea co-culture systems were systematically compared. Their removal capacities for tetracycline, oxytetracycline, and chlortetracycline antibiotics from simulated wastewater were assessed. Chlorella vulgaris-endophytic bacteria-C. rosea co-cultures achieved the best performance under 0.25 mg L-1 antibiotics, which could be further enhanced by GR24 supplementation. This result demonstrates that the combination of endophytic bacteria with microalgae and fungi is superior to activated sludge-B. licheniformis-microalgae-fungi systems. Exogenous supplementation of GR24 is an effective strategy to improve the performance of antibiotics removal from wastewater.

Simultaneous efficient removal of tetracycline and mitigation of antibiotic resistance genes enrichment by a modified activated sludge process with static magnetic field.

To address the increasing issue of antibiotic wastewater, this study applied a static magnetic field (SMF) to the activated sludge process to increase the efficiency of tetracycline (TC) removal from swine wastewater and to reveal its enhanced mechanisms. The results demonstrated that the SMF-modified activated sludge process could achieve almost complete TC removal at sludge loading rates of 0.3 mg TC/g MLSS/d. Analysis of zeta potential and extracellular polymeric substances composition of the activated sludge revealed that SMF increased electrostatic interactions between TC and activated sludge and made activated sludge has much more binding sites, finally resulting in the increased TC biosorption. Metagenomic analysis showed that SMF promoted the enrichment of ammonia-oxidizing bacteria, TC-degrading bacteria, and aromatic compounds-degrading bacteria; it also enhanced ammonia monooxygenase- and cytochrome P450-mediated TC metabolism while upregulating functional genes associated with oxidase, reductase, and dehydrogenase - all contributing to increased TC biodegradation. Additionally, SMF mitigated the enrichment and spread of antibiotic resistance genes (ARGs) by decreasing the abundance of potential hosts of ARGs and inhibiting the upregulation of genes encoding ABC transporters and putative transposase. Based on these findings, this study demonstrates that magnetic field is an enhancement strategy with great potential to relieve the harmful impacts of the growing antibiotic wastewater problem on human health and the ecosystem.

Removal efficiency and abundance of nitrogen cycling microorganism in three bio-matrix materials to treat swine wastewater.

A bio-matrix material (BMM) system is used to pretreat swine wastewater and reduce the nitrogen (N) concentration to the tolerance range of plants in constructed wetlands. In this study, rice straw (RS), wheat straw (WS), and corn stalk (CS) were applied to treat pollutants from swine wastewater, respectively. This one year-long field experiment make up for the lack of long-term experiments and mechanistic investigations of BMM. The pollutant removal efficiency, degradation process of crop straw, and the abundance of nitrogen cycling genes were determined in different BMM systems. The results showed that the removal efficiency of COD, TN, NH4+, and NO3- was the best in the initial 6 months. Furthermore, RS and WS exhibited favorable annual removal efficiency of TN and NH4+, which were 32.81% and 32.99%, 35.3% and 34.97%, respectively. Moreover, the removal efficiency of COD was 30.81% in three BMM systems. Meanwhile, it was found that the dry matter (DM) degradation of crop straws was fast in the first 4-5 months. The degradation rates of cellulose, hemicellulose, and lignin were 94.19%, 94.36%, and 87.32%, respectively, in 1 year. The abundance of nitrogen cycling genes significantly increased by adding BMM, compared with CK (P < 0.05). This showed the abundance of the hzsB gene in RS was the highest, while nirK, nirS, AOA, and AOB were the highest in WS. The addition of RS and WS was better than that of CS in promoting the abundance of nitrogen cycling microorganisms. The results indicated that adding BMM could enhance the anaerobic ammonia oxidation, nitrification, and denitrification. This study not only extends our comprehension of BMM mechanisms in swine wastewater treatment but also serves as a guiding light for numerous farms in similar climate regions.

The Performance and Spatial Distribution of Membrane Fouling in a Sequencing Batch Ceramic Membrane Bioreactor: A Pilot Study for Swine Wastewater Treatment.

The extensive application of ceramic membranes in wastewater treatment draws increasing attention due to their ultra-long service life. A cost-effective treatment for high-strength swine wastewater is an urgent and current need that is a worldwide challenge. A pilot-scale sequencing batch flat-sheet ceramic membrane bioreactor (ScMBR) coupled with a short-cut biological nitrogen removal (SBNR) process was developed to treat high-strength swine wastewater. The ScMBR achieved stable and excellent removal of COD (95.3%), NH4+-N (98.3%), and TN (92.7%), though temperature went down from 20 °C, to 15 °C, to 10 °C stepwise along three operational phases. The COD and NH4+-N concentrations in the effluent met with the discharge standards (GB18596-2001). Microbial community diversity was high, and the genera Pseudomonas and Comamonas were dominant in denitritation, and Nitrosomonas was dominant in nitritation. Ceramic membrane modules of this pilot-scale reactor were separated into six layers (A, B, C, D, E, F) from top to bottom. The total filtration resistance of both the top and bottom membrane modules was relatively low, and the resistance of the middle ones was high. These results indicate that the spatial distribution of the membrane fouling degree was different, related to different aeration scour intensities demonstrated by computational fluid dynamics (CFD). The results prove that the membrane fouling mechanism can be attributed to the cake layer formation of the middle modules and pore blocking of the top and bottom modules, which mainly consist of protein and carbohydrates. Therefore, different cleaning measures should be adopted for membrane modules in different positions. In this study, the efficient treatment of swine wastewater shows that the ScMBR system could be applied to high-strength wastewater. Furthermore, the spatial distribution characteristics of membrane fouling contribute to cleaning strategy formulation for further full-scale MBR applications.

Heavy metal sorption on struvite recovered from livestock wastewaters and release properties of granular forms.

Phosphorus recovery from wastewater is receiving more attention due to its non-renewable property. As copper (Cu) and zinc (Zn) usually occur in livestock wastewater, this study focused on metal sorption in struvite from swine wastewater and the release properties of granular struvite in solution with varying pH conditions (2, 4, 7). The results demonstrated pH values presented a slightly decreasing trend with increasing Cu/Zn ratio, and Zn exhibited higher sorption performance on struvite crystals than that of Cu. Under the high content of metals in the wastewater, Cu/Zn ratios in the wastewater contributed to varying metal binding forms and mechanisms, resulting in the difference in the leaching properties of nutrients and metal. For the granular struvite manufactured with the adhesion of alginate, the P release percentage achieved 30.3-40.5% after 96 h in the wastewater of pH 2, whereas they were only 5.63-8.92% and 1.05-1.50% in the wastewater of pH 4 and 7, respectively. Acid wastewater contributed to the release of two metals, and the release amount of Zn was higher than that of Cu, which is associated with their sorption capacity in crystals. During the latter soil leaching test of adding granular struvite, the NH4+-N and PO43--P concentration in the effluent ranged from 0.34 to 1.26 and 0.62 to 2.56 mg/L after 96 h, respectively. However, the Cu and Zn could not be measured due to lower than the detection limit under varying treatments. Struvite might be accompanied by quicker metal leaching and slower nutrient leaching when surface sorption dominates in wastewater with lower metal concentrations.

Lead Isotope Signatures and Source Identification of Heavy Metals in Vegetable Soils Irrigated with Swine Wastewater of Jiangxi Province, China.

The rapid development of livestock and poultry industry in China has caused serious environment pollution problems. To understand the heavy metals accumulation and identify their sources, 7 heavy metals contents and lead isotope ratios were determined in 24 soil samples from vegetable fields irrigated with swine wastewater in Dongxiang County, Jiangxi Province, China. The results showed that the concentration of Cr, Ni, Cu, Zn, As, Cd and Pb in the swine wastewater irrigated vegetable soils varied from 38.5 to 86.4, 7.57 to 30.6, 20.0 to 57.1, 37.5 to 174, 9.18 to 53.1, 0.043 to 0.274 and 12.8 to 37.1 mg/kg, respectively. The soils were moderately to heavily polluted by As, moderately polluted by Cr, Ni, Cu, Zn and Cd, and unpolluted to moderately polluted by Pb. Sampling soils were classified as moderately polluted according to the Nemerow comprehensive pollution index. Lead isotope and Principal Component Analysis (PCA) analysis indicated that swine wastewater irrigation and atmospheric deposition were the primary sources of the heavy metals.

Carbon flow, energy metabolic intensity and metagenomic characteristics of a Fe (III)-enhanced anerobic digestion system during treating swine wastewater.

Deep treatment and bioenergy recovery of swine wastewater (SW) are beneficial for constructing a low-carbon footprint and resource-recycling society. In this study, Fe (III) addition from 0 to 600 mg/L significantly increased the methane (CH4) content of the recovered biogas from 61.4 ± 2.0 to 89.3 ± 2.0 % during SW treatment in an anaerobic membrane digestion system. The specific methane yields (SMY) also increased significantly from 0.20 ± 0.05 to 0.29 ± 0.02 L/g COD. Fe (III) and its bio-transformed products which participated in establishing direct interspecific electron transfer (DIET), upregulated the abundance of e-pili and Nicotinamide adenine dinucleotide (NADH), enriched electroactive bacteria. The increase in cellular adenosine triphosphate (cATP) from 6583 to 14,518 ng/gVSS and electron transport system (ETS) from 1468 to 1968 mg/(g·h) promoted the intensity of energy flow and electron flow during anaerobic digestion of SW. Moreover, Fe (III) promoted the hydrolysis and acidification of organic matters, and strengthened the acetoacetic methanogenesis pathway. This study established an approach for harvesting high quality bioenergy from SW and revealed the effects and mechanisms from the view of carbon flow, energy metabolic intensity and metagenomics.

Removal of RNA viruses from swine wastewater using anaerobic membrane bioreactor: Performance and mechanisms.

The effective removal of viruses from swine wastewater using anaerobic membrane bioreactor (AnMBR) is vital to ecological safety. However, most studies have focused only on disinfectants, whereas the capabilities of the treatment process have not been investigated. In this study, the performance and mechanism of an AnMBR in the removal of porcine hepatitis E virus (HEV), porcine kobuvirus (PKoV), porcine epidemic diarrhea virus (PEDV), and transmissible gastroenteritis coronavirus (TGEV) are systematically investigated. The results show that the AnMBR effectively removes the four viruses, with average removal efficiencies of 1.62, 3.05, 2.41, and 1.34 log for HEV, PKoV, PEDV and TGEV, respectively. Biomass adsorption contributes primarily to the total virus removal in the initial stage of reactor operation, with contributions to HEV and PKoV removal exceeding 71.7 % and 68.2 %, respectively. When the membrane is fouled, membrane rejection dominated virus removal. The membrane rejection contribution test shows the significant contribution of membrane pore foulants (23-76 %). Correlation analysis shows that the surface characteristics and size differences of the four viruses contribute primarily to their different effects on biomass adsorption and membrane rejection. This study provides technical guidance for viral removal during the treatment of high-concentration swine wastewater using an AnMBR.

Impact of historical soil management on the interaction of plant-growth-promoting bacteria with maize (Zea mays L.).

Edaphic factors can modulate the effects of microbial inoculants on crop yield promotion. Given the potential complexity of microbial inoculant responses to diverse soil management practices, we hypothesize that sustainable management of soil and water irrigation may improve soil quality and enhance the effects of plant growth-promoting bacteria (PGPB). Consequently, the primary objective was to assess the effectiveness of microbial inoculants formulated with Herbaspirillum seropedicae (Hs) and Azospirillum brasilense (Ab) on maize growth in soils impacted by different historical conservation management systems. We evaluated two soil management systems, two irrigation conditions, and four treatments: T0 - without bioinoculant and 100% doses of NPK fertilization; T1 - Hs + humic substances and 40% of NPK fertilization; T2 - Ab and 40% of NPK fertilization; T3 - co-inoculation (Hs + Ab) and 40% of NPK fertilization. Using a reduced fertilization dose (40% NPK) associated with microbial inoculants proved efficient in increasing maize shoot dry mass : on average, there was a 16% reduction compared to the treatment with 100% fertilization. In co-inoculation (Hs + Ab), the microbial inoculants showed a mutualistic effect on plant response, higher than isolate ones, especially increasing the nitrogen content in no-tillage systems irrigated by swine wastewater. Under lower nutrient availability and higher biological soil quality, the microbial bioinputs positively influenced root development, instantaneous water use efficiency, stomatal conductance, and nitrogen contents.

Recent advances in swine wastewater treatment technologies for resource recovery: A comprehensive review.

Swine wastewater (SW), characterized by highly complex organic and nutrient substances, poses serious impacts on aquatic environment and public health. Furthermore, SW harbors valuable resources that possess substantial economic potential. As such, SW treatment technologies place increased emphasis on resource recycling, while progressively advancing towards energy saving, sustainability, and circular economy principles. This review comprehensively encapsulates the state-of-the-art knowledge for treating SW, including conventional (i.e., constructed wetlands, air stripping and aerobic system) and resource-utilization-based (i.e., anaerobic digestion, membrane separation, anaerobic ammonium oxidation, microbial fuel cells, and microalgal-based system) technologies. Furthermore, this research also elaborates the key factors influencing the SW treatment performance, such as pH, temperature, dissolved oxygen, hydraulic retention time and organic loading rate. The potentials for reutilizing energy, biomass and digestate produced during the SW treatment processes are also summarized. Moreover, the obstacles associated with full-scale implementation, long-term treatment, energy-efficient design, and nutrient recovery of various resource-utilization-based SW treatment technologies are emphasized. In addition, future research prospective, such as prioritization of process optimization, in-depth exploration of microbial mechanisms, enhancement of energy conversion efficiency, and integration of diverse technologies, are highlighted to expand engineering applications and establish a sustainable SW treatment system.

Bubble Turbulent Gas-Permeable Membrane for Ammonia Recovery from Swine Wastewater: Mass Transfer Enhancement and Antifouling Mechanisms.

Recovering ammonium from swine wastewater employing a gas-permeable membrane (GM) has potential but suffers from the limitations of unattractive mass transfer and poor-tolerance antifouling properties. Turbulence is an effective approach to enhancing the release of volatile ammonia from wastewater while relying on interfacial disturbance to interfere with contaminant adhesion. Herein, we design an innovative gas-permeable membrane coupled with bubble turbulence (BT-GM) that enhances mass transfer while mitigating membrane fouling. Bubbles act as turbulence carriers to accelerate the release and migration of ammonia from the liquid phase, increasing the ammonia concentration gradient at the membrane-liquid interface. In comparison, the ammonium mass transfer rate of the BT-GM process applied to real swine wastewater is 38% higher than that of conventional GM (12 h). Through a computational fluid dynamics simulation, the turbulence kinetic energy of BT-GM system is 3 orders of magnitude higher than that of GM, and the effective mass transfer area is nearly 3 times that of GM. Seven batches of tests confirmed that the BT-GM system exhibits remarkable antifouling ability, broadens its adaptability to complex water quality, and practically promotes the development of sustainable resource recycling.

Effect of wheat crops on the persistence and attenuation of antibiotic resistance genes in soil after swine wastewater application.

Swine wastewater (SW) application introduces antibiotic resistance genes (ARGs) into farmland soils. However, ARG attenuation in SW-fertigated soils, especially those influenced by staple crops and soil type, remains unclear. This study investigated twelve soil ARGs and one mobile genetic element (MGE) in sandy loam, loam, and silt loam soils before and after SW application in wheat-planted and unplanted soils. The results revealed an immediate increase in the abundance of ARGs in soil by two orders of magnitude above background levels following SW application. After SW application, the soil total ARG abundance was attenuated, reaching background levels at 54 days; However, more individual ARGs were detected above the detection limit than pre-application. Among the 13 genes, acc(6')-lb, tetM, and tetO tended to persist in the soil during wheat harvest. ARG half-lives were up to four times longer in wheat-planted soils than in bare soils. Wheat planting decreased the persistence of acc(6')-lb, ermB, ermF, and intI2 but increased the persistence of others such as sul1 and sul2. Soil type had no significant impact on ARG and MGE fates. Our findings emphasize the need for strategic SW application and the consideration of crop cultivation effects to mitigate ARG accumulation in farmland soils.

Growth of Scenedesmus obliquus in anaerobically digested swine wastewater from different cleaning processes for pollutants removal and biomass production.

Anaerobically digested swine wastewater (ASW) purification by microalgae provides a promising strategy for nutrients recovery, biomass production and CO2 capture. However, the characteristics of ASW from different cleaning processes vary greatly. At present, the cultivation of microalgae in ASW from different manure cleaning processes is rarely investigated and compared. That may bring uncertainty for microalgae growth using different ASW in large-scale application. Thus, the ASW from three cleaning processes were tested for cultivating microalgae, including manure dry collection (I), water flushing (II) and water submerging processes (III). The characteristics of ASW from three manure cleaning processes varied greatly such as nutrient and heavy metals levels. High concentration of ammonia and copper in ASW significantly inhibited microalgae growth. Fortunately, the supply of high CO2 (10%) effectively alleviated negative influences, ensuring microalgal growth at low dilution ratio. The characteristics of three ASW resulted in significant differences in microalgae growth and biomass components. The maximal biomass production in optimal diluted ASW-I, II and III reached 1.46 g L-1, 2.19 g L-1 and 2.47 g L-1, respectively. The removal of organic compounds, ammonia and phosphorus by optimal microalgae growth in diluted ASW-I, II and III was 50.6%/94.2%/64.7%, 63.7%/82.3%/57.6% and 83.2%/91.7%/59.7%, respectively. The culture in diluted ASW-I, II and III obtained the highest lipids production of 12.1 mg L-1·d-1, 16.5 mg L-1·d-1 and 19.4 mg L-1·d-1, respectively. The analysis of lipids compositions revealed that the proportion of saturated fatty acids accounted for 36.4%, 32.4% and 27.9 % in optimal diluted ASW-I, II and III, as ideal raw materials for biodiesel production.

Electrochemical route outperforms chemical struvite precipitation in mitigating heavy metal contamination.

Electrochemically mediated struvite precipitation (EMSP) offers a robust, chemical-free process towards phosphate and ammonium reclamation from nutrients-rich wastewater, i.e., swine wastewater. However, given the coexistence of heavy metal, struvite recovered from wastewater may suffer from heavy metal contamination. Here, we systematically investigated the fate of Cu2+, as a representative heavy metal, in the EMSP process and compared it with the chemical struvite precipitation (CSP) system. The results showed that Cu2+ was 100% transferred from solution to solid phase as a mixture of copper and struvite under pHi 9.5 with 2-20 mg/L Cu2+ in the CSP system, and varying pH would affect struvite production. In the EMSP system, the formation of struvite was not affected by bulk pH, and struvite was much less polluted by co-removed Cu2+ (24.4%) at pHi 7.5, which means we recovered a cleaner and safer product. Specifically, struvite mainly accumulates on the front side of the cathode. In contrast, the fascinating thing is that Cu2+ is ultimately deposited primarily to the back side of the cathode in the form of copper (hydro)oxides due to the distinct thickness of the local high pH layer on the two sides of the cathode. In turn, struvite and Cu (hydro)oxides can be harvested separately from the front and back sides of the cathode, respectively, facilitating the subsequent recycling of heavy metals and struvite. The contrasting fate of Cu2+ in the two systems highlights the merits of EMSP over conventional CSP in mitigating heavy metal pollution on recovered products, promoting the development of EMSP technology towards a cleaner recovery of struvite from waste streams.

A pilot-scale SNAD-MBBR process for treating anaerobic digester liquor of swine wastewater: performance and microbial community.

In this pilot-scale study, simultaneous partial nitrification, anammox, and denitrification (SNAD) process was achieved successfully in a moving bed biofilm reactor (MBBR) for treating anaerobic digester liquor of swine wastewater. After 95 days of operation, when the total nitrogen loading rate of SNAD-MBBR process was 1.09 kg TN/m3/day, the total nitrogen removal rate could reach 0.87 kg TN/m3/day, and the removal efficiencies of ammonium and total nitrogen were 92.0% and 79.7%, respectively. The optimum pH and temperature for SNAD-MBBR process were 8.5 and 35 °C, respectively, and the optimum dissolved oxygen for SNAD1 and SNAD2 were 0.30 and 0.07 mg/L, respectively. The 16S rRNA sequencing suggested that Candidatus Kuenenia, Candidatus Brocadia, Nitrosomonas, and Denitratisoma were the dominant nitrogen removal bacteria. Some of the co-existing bacteria (Truepera, Limnobacter, and Anaerolineaceae uncultured) promoted ammonium oxidation and guaranteed the growth of the anammox bacteria under adverse environmental conditions. Overall, this study demonstrated that the SNAD-MBBR process would be an energy-saving and cost-effective method for the removal of nitrogen from swine wastewater and provided important process parameters for stable operation of the full-scale SNAD process.

Low-cost materials for swine wastewater treatment using adsorption and Fenton's process.

Untreated swine wastewater (SW) discharge leads to serious consequences such as water quality decreasing related to eutrophication and proliferation of harmful algae containing cyanotoxins, which can cause acute intoxication in humans. The use of untreated pig farming effluent as fertilizer can lead to the accumulation of polluting compounds. Biological treatments can degrade organic matter but have the disadvantage of requiring large areas and high retention times and demonstrating low efficiencies in the degradation of refractory compounds such as pharmaceutical compounds. In this ambit, the performance of four low-cost materials was evaluated for treatment of a swine wastewater using physical-chemical processes such as adsorption and Fenton's process. The tested materials are two natural resources, red volcanic rock from Canary (RVR) Islands and black volcanic rock (BVR) from Azores, and two industry residues, red mud (RM) and iron filings (IF). Among the tested materials, only IFs are catalytically active for Fenton's peroxidation. Still, RVR, BVR, and RM were efficient adsorbents removing up to 67% of COD. The combination between adsorption followed by Fenton's process using IF as catalyst showed interesting results. When RM is applied as adsorbent in the diluted effluent, it was able to remove 67% and 90% of COD for adsorption and adsorption followed by IF Fenton, respectively. At those conditions, the resultant treated effluent accomplishes the requirements for direct discharge in the natural water courses as well as the parameters for water reusing.