Analysis of per- and poly-fluoroalkyl substances (PFAS) in processed foods from FDA's Total Diet Study.

Additional occurrence data are needed to better understand human exposure to per- and poly-fluoroalkyl substances (PFAS) from commercially available foods in the United States. The Food and Drug Administration's (FDA) Total Diet Study (TDS) collects foods that are both nationally and regionally distributed. In 2018, 172 processed foods were collected from grocery stores around Lenexa, KS, as part of the TDS national collection. A previously developed method for the analysis of PFAS in foods as part of the TDS regional collection was modified and optimized for these samples. This method was single lab validated using 5 different matrices and method detection limits were calculated. During the analysis of these samples, challenges arose with method blanks and further investigation into statistical methods to distinguish between blank and sample concentrations were done. The confirmation of two short chain PFAS, perfluorobutanoic acid (PFBA) and perfluoropentanoic acid (PFPeA), was not possible using triple quadrupole mass spectrometry and a confirmation method was developed using high-resolution mass spectrometry. This technique was also used to investigate potential detections and interferents that fell within the retention time criteria for positive detections. In the national collection, positive detections of perfluorooctanesulfonic acid (PFOS) and perfluorononanoic acid (PFNA) were found in frozen fish sticks/patties, PFOS and perfluorodecanoic acid (PFDA) in canned tuna, and PFOS in protein powder. Concentrations were all below 150 ppt, and no other detects were confirmed above the method detection limits in any other foods.

Impact of Intestinal Concentration and Colloidal Structure on the Permeation-Enhancing Efficiency of Sodium Caprate in the Rat.

In this work, we set out to better understand how the permeation enhancer sodium caprate (C10) influences the intestinal absorption of macromolecules. FITC-dextran 4000 (FD4) was selected as a model compound and formulated with 50-300 mM C10. Absorption was studied after bolus instillation of liquid formulation to the duodenum of anesthetized rats and intravenously as a reference, whereafter plasma samples were taken and analyzed for FD4 content. It was found that the AUC and Cmax of FD4 increased with increasing C10 concentration. Higher C10 concentrations were associated with an increased and extended absorption but also increased epithelial damage. Depending on the C10 concentration, the intestinal epithelium showed significant recovery already at 60-120 min after administration. At the highest studied C10 concentrations (100 and 300 mM), the absorption of FD4 was not affected by the colloidal structures of C10, with similar absorption obtained when C10 was administered as micelles (pH 8.5) and as vesicles (pH 6.5). In contrast, the FD4 absorption was lower when C10 was administered at 50 mM formulated as micelles as compared to vesicles. Intestinal dilution of C10 and FD4 revealed a trend of decreasing FD4 absorption with increasing intestinal dilution. However, the effect was smaller than that of altering the total administered C10 dose. Absorption was similar when the formulations were prepared in simulated intestinal fluids containing mixed micelles of bile salts and phospholipids and in simple buffer solution. The findings in this study suggest that in order to optimally enhance the absorption of macromolecules, high (≥100 mM) initial intestinal C10 concentrations are likely needed and that both the concentration and total dose of C10 are important parameters.

Ketogenic Diet: Impact on Cellular Lipids in Hippocampal Murine Neurons.

BACKGROUND: The mechanism of action of the ketogenic diet (KD), an effective treatment for pharmacotherapy refractory epilepsy, is not fully elucidated. The present study examined the effects of two metabolites accumulating under KD-beta-hydroxybutyrate (ßHB) and decanoic acid (C10) in hippocampal murine (HT22) neurons. METHODS: A mouse HT22 hippocampal neuronal cell line was used in the present study. Cellular lipids were analyzed in cell cultures incubated with high (standard) versus low glucose supplemented with ßHB or C10. Cellular cholesterol was analyzed using HPLC, while phospholipids and sphingomyelin (SM) were analyzed using HPTLC. RESULTS: HT22 cells showed higher cholesterol, but lower SM levels in the low glucose group without supplements as compared to the high glucose groups. While cellular cholesterol was reduced in both ßHB- and C10-incubated cells, phospholipids were significantly higher in C10-incubated neurons. Ratios of individual phospholipids to cholesterol were significantly higher in ßHB- and C10-incubated neurons as compared to controls. CONCLUSION: Changes in the ratios of individual phospholipids to cholesterol in HT22 neurons suggest a possible alteration in the composition of the plasma membrane and organelle membranes, which may provide insight into the working mechanism of KD metabolites ßHB and C10.

Exposure to Perflouroalkyl acids and foetal and maternal thyroid status: a review.

BACKGROUND: Exposure to perfluorinated-alkyl-acids (PFAAs) is ubiquitous. PFAAs are hormone-disrupting compounds that are strongly suspected to affect mother-child-health such as fetal growth. Thyroid disruption is a plausible mechanism of action. We aim to summarize the epidemiological evidence for the relation between prenatal and postnatal exposure to PFAAs and disruption of thyroid homeostasis in mothers and/or infants. METHOD: Fifteen original publications on PFAAs concentrations and thyroid hormones (TH) in pregnant women and/or infants were found upon a literature search in the PubMed database. Information on exposure to seven PFAAs congeners [Perfluorooctane sulfonate (PFOS), Perfluorooctanoate (PFOA), Perfluorohexane sulfonate (PFHxS), Perfluorononanoic acid (PFNA), Perfluorodecanoic acid (PFDA), Perfluoroundecanoic acid (PFUnA), and Perfluorododecanoic acid (PFDoA)] and thyroid stimulating hormone (TSH), free and total thyroxine (FT4 and TT4), free and total triiodothyronine (FT3 and TT3), T3RU (Free triiodothyronine resin uptake) and FT4-index (FT4I) levels were recorded. We evaluated sampling of maternal TH by trimester, and infant TH by sex stratification. Reported associations between mother or infant PFAAs and TH were not uniformly assessed in the selected studies. RESULTS: Ten out of the fifteen studies examined maternal PFAAs concentration and TSH level. Seven studies showed significant associations between TSH and exposure to six PFAAs congeners, most of them were positive. Maternal T4 and T3 were investigated in nine studies and five studies found inverse associations between exposure to six PFAAs congeners and TH (TT3, TT4, FT3, FT4 and FT4I) levels. Eight of the fifteen studies investigated PFAAs concentrations and infant TSH. Infant TSH level was significantly affected in four studies, positively in three studies. Nine studies investigated infant T4 and T3 and seven studies found significant associations with PFAAs exposure. However, both inverse and positive significant associations with infant TH were found eliciting no clear direction. CONCLUSION: Results indicate a mainly positive relationship between maternal PFAAs concentrations and TSH levels, and suggestion of an inverse association with T4 and/or T3 levels. Associations of infant TH with PFAAs concentration were less consistent.

The Relationship Between Perfluoroalkyl Substances Concentrations and Thyroid Function in Early Childhood: A Prospective Cohort Study.

Background: Exposure to perfluoroalkyl substances (PFAS) has been suggested to affect thyroid function; however, data on early-life exposure and thyroid function in early childhood are scarce. We investigated the cross-sectional and longitudinal relationships of early-life exposure to PFAS with thyroid function at 2, 4, and 6 years of age. Methods: This study used data on PFAS exposure and thyroid function from the Environment and Development of Children (EDC) cohort study. A total of 660 children who visited at least once at 2, 4, or 6 years of age (381 children aged 2 years, 569 children aged 4 years, and 511 children aged 6 years) were included in this study. Serum thyrotropin (TSH) levels were measured at 2, 4, and 6 years of age. The relationship of serum PFAS (sPFAS) concentrations with TSH levels at the three time points was assessed by repeated-measure analysis using linear mixed models. The serum levels of free thyroxine (fT4) and triiodothyronine (T3) were measured once (at 6 years of age). The relationship of sPFAS with fT4 and T3 levels at 6 years of age was investigated by linear regression analyses. Results: None complained of hyper- or hypothyroid symptoms with normal fT4 and T3 levels. Repeated-measure analysis showed that TSH levels at 2, 4, and 6 years of age were inversely associated with serum perfluorononanoic acid (sPFNA), after adjusting for age, sex, and/or dietary iodine intake (p < 0.05). When stratified by sex, TSH levels were inversely associated with serum perfluorooctanoic acid (sPFOA) in boys and sPFNA in girls (p < 0.05 for both). fT4 levels at 6 years of age were positively related to sPFNA and serum perfluorohexane sulfonic acid at 2 years of age and sPFOA at 6 years of age, and T3 levels at 6 years of age showed positive relationships with serum perfluorodecanoic acid and serum perfluorooctane sulfonic acid at 6 years of age (p < 0.05 for all). When stratified by sex, similar positive relationships for sPFAS with fT4 and T3 levels were significant among boys only. Conclusions: A significant relationship was found between early-life exposure to PFAS and thyroid function. Early-life exposure to PFAS was associated with decreased TSH and increased fT4 or T3 levels among preschool-age children.

Quantifying plasticizer leakage from ion-selective membranes - a nanosponge approach.

The spontaneous process of release of plasticizers from membranes typically used in ion-selective sensors is an effect which limits the lifetime of sensors and comes with a risk of safety hazards. We use a nanosponge approach to look at the magnitude of this problem, quantifying the resulting contents of the plasticizer in solution. This novel method takes advantage of the spontaneous partition of the plasticizer (released and present in solution) into nanoparticles loaded with a solvatochromic dye. As a result, nanoparticles are transformed into capsules. This process is coupled with the turn-on fluorescence intensity change of the dye embedded in nanostructures, proportional to analyte concentration in the ppm range, providing insight into plasticizer contents in the solution. It was found that the spontaneous release of the plasticizer is dependent on its nature as well as the presence of an ionophore and ion-exchanger. For a typical ion-selective membrane composition the leakage effect results in up to 20 ppm of 2-nitrophenyl octyl ether found in solution after 12 h contact. On the other hand, for a less polar plasticizer - bis(2-ethylhexyl) sebacate, although the presence of an ionophore and ion-exchanger also increases the amount of the compound released from the membrane, its concentration in solution does not exceed 2 ppm after 12 h. The conclusions presented herein can be important not only for designing robust sensors but also for end-user safety. The results obtained for ion-selective membranes were equal within the range of experimental errors with those obtained using a liquid chromatography coupled with mass spectrometry (LC MS) approach, confirming the high analytical potential of the nanosponge approach.

Impact of Extended Lactation on Fatty Acid Profile and Milk Composition of Dual Purpose Tropical Goat.

BACKGROUND AND OBJECTIVE: Extended lactation has been implemented to increase milk yield in limited case. There is need further investigation in term of milk composition, fatty acid profile and milk flavour as recommendation for further implementation. MATERIALS AND METHODS: The study lasted from July-September, 2017, used thirty lactating Etawah Crossbred goats in small farms of Sleman, Yogyakarta. Samples were collected from normal period and extended lactation which lasted for 2-3 months and longer than 10 months, respectively. The data on milk yield, composition, physical quality, fatty acids profile, sensory properties and nutrient consumption were measured on samples of 14 consecutive days. Assessments were done in Faculty of Animal Science and Integrated Research Laboratory, Universitas Gadjah Mada. Statistical analysis used ANOVA and Kruskal Wallis test and were processed with SPSS programme version 16. RESULTS: Extended lactation did not affect milk yield and nutrient consumption of goat but caused higher content of milk fat, protein, total solid, acidity, caprylic, capric and total short-chain fatty acids in compared with normal lactation (p<0.05). The average values were 5.33, 4.40, 15.85, 0.30, 1.99, 9.10 and 12.13% vs. 3.75, 3.64, 13.55, 0.26, 1.69, 7.09 and 9.76% of total fatty acids in milk, respectively. Fatty acid profile did not associate with milk flavour. CONCLUSION: Composition, acidity and short chain fatty acids content in milk of extended lactation were higher than in normal period but did not change milk flavour.

Perfluorinated Compounds (PFCs) in Soil of the Pearl River Delta, China: Spatial Distribution, Sources, and Ecological Risk Assessment.

This study investigated the levels, spatial distribution, sources, and ecological risks of 16 perfluorinated compounds (PFCs) in 68 surface soil samples (0-20 cm) from 7 cities in the Pearl River Delta (PRD), China. Sixteen target PFCs, including perfluoroalkyl carboxylic acids (PFCAs, C5-C14, C16, and C18) and perfluoroalkyl sulfonic acids (PFSAs, C4, C6, C8, and C10), were analyzed by high-performance liquid chromatography-negative electrospray ionization-tandem mass spectrometry (HPLC/ESI-MS/MS). Concentrations of total PFCs (∑PFCs) ranged from 2.19 to 98.5 µg kg-1 (dry weight, dw), with an average of 5.97 µg kg-1 dw. Perfluorooctane sulfonate (PFOS) was the dominant PFC, accounting for 23.9% of ∑PFCs. The highest ∑PFCs was found in the soil sample collected from Dongguan with a large number of manufacturing industries. There were no significant differences of ∑PFCs among unban, industrial, and agricultural soils, indicating similar pollution sources in soil of the PRD. More than 70% of ∑PFCs in soil of the PRD could be attributed to the four principal components, represented by PFOS and perfluorooctanoic acid (PFOA), perfluoropentanoic acid (PFPeA) and perfluorohexanoic acid (PFHxA), perfluorodecanoic acid (PFDA), and perfluoroundecanoic acid (PFUdA). Ecological risk assessment indicated that PFOA had low risk to soil plants and animals. However, the risk of PFOS to soil plants was relatively high in some studied regions.

Analytical Methodology for Trace Determination of Propoxur and Fenitrothion Pesticide Residues by Decanoic Acid Modified Magnetic Nanoparticles.

A sensitive, rapid, reliable, and easily applicable method based on magnetic solid phase extraction (MSPE) combined with HPLC-PDA was developed for monitoring propoxur (PRO) and fenitrothion (FEN) pesticides in environmental water samples. The effect of major experimental variables on the extraction efficiency of both the pesticides was investigated and optimized systematically. For this purpose, a new magnetic material containing decanoic acid on the surface of particles was synthesized and characterized by XRD, FT-IR, SEM, EDX, and TGA analysis in detail. The simultaneous determination of pesticide molecules was carried out by using a Luna Omega C18 column, isocratic elution of acetonitrile (ACN): Water (70:30 v/v) with a flow rate of 1.2 mL min-1. After MSPE, the linear range for pesticide molecules (r2 > 0.9982) was obtained in the range of 5-800 and 10-800 ng mL-1, respectively. The limit of detections (LOD) are 1.43 and 4.71 ng mL-1 for PRO and FEN, respectively while RSDs % are below 3.5%. The applicability of the proposed method in four different environmental samples were also investigated using a standard addition-recovery procedure. Average recoveries at two spiking levels were over the range of 91.3-102.5% with RSD < 5.0% (n = 3). The obtained results show that decanoic acid grafted magnetic particles in MSPE combined with HPLC-PDA is a fast and simple method for the determination of PRO and FEN in environmental water samples.

Perfluoroalkyl substances (PFASs) in air-conditioner filter dust of indoor microenvironments in Greece: Implications for exposure.

The occurrence of perfluoroalkyl substances (PFASs) was for the first time investigated in various working microenvironments (internet cafes, electronics shops, coffee shops, restaurants, etc.) in Thessaloniki, Greece, using the dust trapped by central air conditioner (A/C) filters. Perfluorooctane sulfonic acid (PFOS) was found in the range from 16 to 227 ng g-1, however it was detectable in only 30% of samples. On the contrary, perfluorohexanoic acid (PFHxA) was found in 85% of samples in the range from 3.6 to 72.5 ng g-1, while 90-95% of samples exhibited perfluorooctanoic acid (PFOA), perfluorodecanoic acid (PFDcA) and perfluorododecanoic acid (PFDoDA) in the range from 10-653 ng g-1, 3.2-7.4 ng g-1 and 3.8-13.1 ng g-1, respectively. The PFAS profile varied largely among the different microenvironment categories suggesting different sources. Estimated daily intakes through dust ingestion were calculated.

Occurrences, levels and risk assessment studies of emerging pollutants (pharmaceuticals, perfluoroalkyl and endocrine disrupting compounds) in fish samples from Kalk Bay harbour, South Africa.

A comprehensive analysis of 15 target chemical compounds (pharmaceuticals and personal care product, perfluoroalkyl compounds and industrial chemicals) were carried out to determine their concentrations in selected commercially exploited, wild caught small and medium sized pelagic fish species and their organs (Thyrsites atun (snoek), Sarda orientalis (bonito), Pachymetopon blochii (panga) and Pterogymnus laniarius (hottentot)) obtained from Kalk Bay harbour, Cape Town. Solid phase extraction (SPE) method based on Oasis HLB cartridges were used to concentrate and clean-up the samples. Liquid chromatography-mass spectrometry analysis of these chemical compounds revealed the simultaneous presence of at least 12 compounds in different parts of the selected fish species in nanogram-per-gram dry weight (ng/g dw) concentrations. The results revealed that perfluorodecanoic acid, perfluorononanoic acid and perfluoroheptanoic acid were the most predominant among the perfluorinated compounds and ranged between: (20.13-179.2 ng/g), (21.22-114.0 ng/g) and (40.06-138.3 ng/g). Also, diclofenac had the highest concentration in these edible fish species out of all the pharmaceuticals detected (range: 551.8-1812 ng/g). The risk assessment values were above 0.5 and 1.0 for acute and chronic risk respectively which shows that these chemicals have a high health risk to the pelagic fish, aquatic organisms and to humans who consume them. Therefore, there is an urgent need for a precautionary approach and the adequate regulation of the use and disposal of synthetic chemicals that persist in aquatic/marine environment in this province and other parts of South Africa, to prevent impacts on the sustainability of our marine environment, livelihood and lives.

Are perfluoroalkyl substances in water and fish from drinking water source the major pathways towards human health risk?

Due to potential adverse effects and bioaccumulation in biota and humans, perfluoroalkyl substances (PFASs) have raised wide attention in recent years. Ingestion is a vital pathway for PFASs to transmit to humans especially through water and fish. In present study, PFASs in water and fish from the drinking water source of Beijing in China were investigated. Three layers of water were collected in order to find the connection between concentrations of PFASs and depth of water, which showed no prominent correlation. PFASs in water from Miyun Reservoir with concentrations of 5.30-8.50 ng/L, were relatively lower compared with other reports on raw drinking water. Perfluorobutanoic acid (PFBA) and perfluorooctanoic acid (PFOA) were the dominant PFASs. In addition, six species of fish (including Cyprinus carpio, Carassius auratus, Erythroculter dabryi, Pseudohemiculter dispar, Hypophthalmichthys molitrix and Siniperca chuatsi) were analyzed, with concentrations of PFASs ranging from 1.70 to 14.32 ng/g wet weight (w.w.). Due to relatively stronger bioaccumulation potential, long chain perfluorinated carboxylates (PFCAs) and perfluorinated sulfonates (PFSAs) were detected with higher concentrations, especially perfluoroundecanoic acid (PFUdA) and perfluorodecanoic acid (PFDA). The estimated daily intake (EDI) of PFASs through drinking water and fish consumption were 0.20-0.34 and 3.44-12.61 ng/kg bw/day based on Exposure Factors Handbook of Chinese Population, respectively. In addition, the EDI of high-priority concern PFASs via pork, chicken and dust were also calculated, with value of 0.015-0.043, 0.003-0.013 and 0.074-0.390 ng/kg bw/day, respectively. The total EDI of PFOS and PFOA via diverse pathways were less than suggested tolerable daily intake (PFOS, 150 ng/kg bw/day; PFOA, 1500 ng/kg bw/day), indicating that the detected levels would not cause severe health effects on Beijing residents.

Accumulation of perfluoroalkyl substances in lysimeter-grown rice in Japan using tap water and simulated contaminated water.

Perfluoroalkyl substances (PFASs) are a group of contaminants of concern in agricultural crops, but little is known of their accumulation or behavior in grains. We grew Japanese rice (Oryza sativa subsp. indica) in lysimeters irrigated with tap water or tap water plus simulated contaminated water for 2 years, then analyzed the roots, straw, unhulled rice, white rice, bran, soil, and water for PFASs residues. Total fluorine was measured by combustion ion chromatography. Estimated per-plant residue levels were 3.0 pg perfluorooctanesulfonic acid (PFOS) (bran: 0.5%, hull: 99.5%), 0.54 pg N-ethylperfluorooctanesulfonamide (N-EtFOSA) (white rice: 67%, hull: 33%), 1.2 pg perfluorobutanoic acid (PFBA) (white rice: 13%, bran: 7%, hull: 79%), 0.68 pg perfluoropentanoic acid (hull: 100%), 0.50 pg perfluorohexanoic acid (PFHxA) (white rice: 65%, bran: 16%, hull: 19%), 0.21 pg perfluoroheptanoic acid (hull: 100%), 0.25 pg perfluorooctanoic acid (PFOA) (hull: 100%), and 0.12 pg perfluorodecanoic acid (PFNA) (white rice: 81%, bran: 19%). Estimated daily PFASs intakes were <1-3 ng perfluorooctanesulfonamide, <1-7 ng N-EtFOSA, 1-2 ng PFBA, <3-4 ng PFHxA, and 1-2 ng PFNA. Estimated PFOS, PFOA, and total PFASs in straw feed were 0.4, 0.1, and 2 kg yr-1 and 0.7, 0.4, and 8 kg yr-1 in 2015 and 2016, respectively. Estimated PFOS, PFOA, and total PFASs in straw fertilizer were 4, 1, and 23 kg yr-1 and 7, 4, and 86 kg yr-1 in 2015 and 2016, respectively. PFASs accumulation may cause longer residence time in agricultural systems owing to straw being used as animal feed and organic fertilizer.

Volatomic pattern of breast cancer and cancer-free tissues as a powerful strategy to identify potential biomarkers.

Breast cancer (BC), ranked as the fifth amongst all cancers, remains at the top of women's cancers worldwide followed by colorectal, lung, cervix, and stomach cancers. The main handicap of most of the screening/diagnostic methods is based on their low sensitivity and specificity and the invasive behavior of most sampling procedures. The aim of this study was to establish the volatomic pattern of BC and cancer-free (CF) tissues (n = 30) from the same patients, as a powerful tool to identify a set of volatile organic metabolite (VOM) potential BC biomarkers which might be used together or complement with the traditional BC diagnostics strategies, through the integration of chromatographic data, obtained by solid-phase microextraction followed by gas chromatography-mass spectrometry (SPME/GC-qMS), with chemometric tools. A total of four metabolites: limonene, decanoic acid, acetic acid and furfural presented the highest contribution towards discrimination of BC and CF tissues (VIP > 1, p < 0.05). The discrimination efficiency and accuracy of BC tissue metabolites was ascertained by ROC curve analysis that allowed the identification of some metabolites with high sensitivity and specificity. The results obtained with this approach suggest the possibility of identifying endogenous metabolites as a platform to find potential BC biomarkers and pave the way to investigate the related metabolomic pathways in order to improve BC diagnostic tools. Moreover, deeper investigations could unravel novel mechanistic insights into the disease pathophysiology.

Does soil track-in contribute to house dust concentrations of perfluoroalkyl acids (PFAAs) in areas affected by soil or water contamination?

The Minnesota Department of Health measured levels of perfluoroalkyl acids (PFAAs) in house dust at homes in communities impacted by PFAA-contaminated soil and drinking water to determine whether PFAAs in soil outside the home are associated with concentrations in dust. House dust samples from both interior living spaces and entryways to the yard were collected and analyzed separately based on the presumption that PFAAs in entryway dust may better reflect "track-in" of PFAAs into the home from contaminated soil or lawns irrigated with contaminated water. PFAA detections and concentrations in living rooms were significantly higher compared to entryways; and concentrations in both sampling locations were higher than corresponding soil concentrations, suggesting that interior sources were the main contributors to PFAAs in house dust. PFAA dust concentrations in entryways were significantly associated with living room dust levels for all analytes except PFBA. Relationships between entryway dust and soil were only seen for one PFAA (PFOA). However, median concentrations of PFOA in entryway and living room dust were 35 and 70 times higher (respectively) than in soil, which highlights the lack of importance of PFAA soil track-in as a contributor to dust concentration in this setting. Due to the small sample size, larger scale studies are needed to further assess the potential for migration of PFAA contaminated soil to indoor dust.

Occurrence and partitioning behavior of perfluoroalkyl acids in wastewater effluent discharging into the Long Island Sound.

Perfluoroalkyl acids (PFAAs) were measured in aqueous and suspended particulate matter (SPM) fractions in the final effluents from 12 wastewater treatment facilities located around the Connecticut shoreline. Aqueous phase concentrations ranged from 53 to 198 ng/L for ∑PFAAs with ≤7 perfluorinated carbons (CF2) and 2-73 ng/L for >7 CF2 PFAAs. Predominant PFAAs associated with effluent derived SPM were perfluorodecanoic acid and perflurorooctane sulfonic acid, detected in 48% and 52% of samples in concentrations ranging from 7 CF2 PFAAs. Partitioning coefficients (log KOC) derived for effluent water and SPM phases (4.2 ±â€¯0.3, 4.4 ±â€¯0.4, 5.1 ±â€¯0.2 and 5.3 ±â€¯0.2 for PFOA, PFNA PFDA and PFUnA; 4.5 ±â€¯0.2 and 5.2 ±â€¯0.2 for PFOS and PFHsX respectively) were found to be of similar magnitude to aeration tank particles, though 0.5 to 2 log units greater than sludge solids and to natural system particulates including riverine SPM, estuarine SPM and sediments. Results from this study suggest that effluent derived suspended particulate matter could be an effective vector in the transport of long-chained PFAAs through wastewater treatment into receiving waters, and a potential vector to the local food chain.

Inter-individual, inter-city, and temporal trends of per- and polyfluoroalkyl substances in human milk from Swedish mothers between 1972 and 2016.

Inter-individual, inter-city, and temporal trends of 19 per- and polyfluoroalkyl substances (PFASs) were investigated in human milk collected in Stockholm (1972-2016) and Gothenburg (2007-2015), Sweden. The concentrations of perfluorohexane sulfonate (PFHxS), perfluorononanoate (PFNA), perfluorodecanoate (PFDA), perfluoroundecanoate (PFUnDA), and perfluorotridecanoate (PFTrDA) in human milk from Stockholm increased significantly over the entire monitoring periods, whereas branched (Br) and linear (L) isomers of perfluorooctane sulfonamide (FOSA) decreased. In human milk from Gothenburg, significant downward trends were detected for perfluorododecanoate (PFDoDA), PFHxS and Br-perfluorooctane sulfonate (Br-PFOS) over the last decade. This declining trend was also observed for perfluorohexanoate (PFHxA), PFHxS, perfluorooctanoate (PFOA) and Br-PFOS in Stockholm over the same time period. No significant differences were observed in concentrations or relative PFAS profiles between Stockholm and Gothenburg. However, a comparison of the PFAS profile in Stockholm milk revealed distinct profiles for the time periods 1972-1996, 2000-2012, and 2013-2016, reflecting a shift in exposure over time. The lower bound estimated daily intake (EDI) for ∑PFAS concentrations in infants ranged from 7.1-40 ng per kg body weight per day (ng/kg bw/d) in Stockholm and from 5.2-25 ng/kg bw/d in Gothenburg over the studied time period, consistent with other European countries. Overall these data indicate that exposure to some legacy PFASs via breastmilk is declining, presumably as a result of regulation and phase-out initiatives. However, increasing concentrations for other PFASs and a shift in the overall PFAS profile in recent years may pose an ongoing health risk to infants.

Suitability of lemon balm (Melissa officinalis L.) extract rich in rosmarinic acid as a potential enhancer of functional properties in cupcakes.

Melissa officinalis (lemon balm) and its extracts have been frequently reported as possessing bioactive properties, offering the potential for use in development/enrichment of food products with additional functional capabilities, providing health benefits to consumers. The antioxidant, antibacterial and antifungal activity of lemon balm extract, as well as its potential hepatotoxicity were thoroughly evaluated. The extracts were then incorporated into cupcakes and their preserving effect, chemical composition, colour parameters and antioxidant activity were compared with those provided by potassium sorbate. In general, the variables with the largest differences among different storage times were energy level, sucrose, glucose, palmitic acid (C6:0) and oleic acid (C18:1n9). On the other hand, L∗ (top), a∗ (top), b∗ (top), pH, capric acid (C10:0) and lauric acid (C12:0) showed the greatest variation according to cupcake formulation. The results observed indicate that the lemon balm extract rich in rosmarinic acid can provide advantageous functional properties to bakery products.

High-resolution dynamic computer simulation of electrophoresis using a multiphysics software platform.

The modeling and simulation software COMSOL Multiphysics® was recently extended with an electrophoretic transport interface. Its performance was investigated by comparison to results obtained using the 1D dynamic electrophoresis simulators GENTRANS and SIMUL5. Simulations of zone electrophoresis, isotachophoresis, isoelectric focusing and of an oscillating electrolyte system were performed. Smooth profiles were essentially identical indicating that the COMSOL electrophoretic transport interface is able to reproduce results of the 1D simulators. Differences in the way the respective numerical schemes handle steep concentration gradients and associated instabilities were observed. The COMSOL electrophoretic transport interface is expected to be useful as a general model for simulations in 1D, 2D or 3D geometries, as well as for simulations combining electrophoresis with other physical phenomena.

Metabolic effects of inhaled salbutamol determined by exhaled breath analysis.

We explore whether real-time breath analysis by high resolution mass spectrometry is suitable to monitor changes at the metabolic level due to inhaling bronchodilator medication. We compared the breath levels of metabolites in a group of patients (n = 50) at baseline and 10 and 30 min after inhalation of 200 µg salbutamol. The same procedure was performed with a group of controls (n = 48) inhaling a placebo spray. A total of 131 mass spectral features were significantly altered as a result of inhaling medication, but not after inhaling placebo. We found that homologous series of chemical classes correlated strongly with each other, strengthening the notion that certain biochemical processes can be monitored. For example, a series of fatty acids was found to be increased after salbutamol intake, suggesting lipolysis stimulation. Peaks corresponding to salbutamol, its main metabolite salbutamol-4-O-sulfate and formoterol were found to be generally increased in patients inhaling the drugs on an as-needed basis, as compared to non-medicated volunteers. Overall, these results suggest such real-time breath analysis is a useful tool for non-invasive therapeutic drug monitoring.