RESUMO
In-vitro analysis of venous blood taken from rats irradiated by 300 and 5,000 J/m2 of UV showed no effect on metabolism and, therefore, energy and recovery systems of erythrocytes. Concentrations of 2,3-diphosphoglycerate and reduced glutathione were increased after irradiation by 5,000 J/m2. UV-irradiation at 10,000 J/m2 decreased adenosine triphosphate and phospholipids in blood and impaired the functional stability of erythrocyte membranes. Recovery of the membrane structure in 24 hrs. after irradiation suggests extended photochemical processes in cells and is consistent with the literary data about indirect effects of plasma proteins on the red cell function.
Assuntos
Membrana Eritrocítica/metabolismo , Membrana Eritrocítica/efeitos da radiação , Raios Ultravioleta , Adaptação Fisiológica/fisiologia , Adenosina Trifosfatases/metabolismo , Adenosina Trifosfatases/efeitos da radiação , Animais , Permeabilidade da Membrana Celular/efeitos dos fármacos , Ácidos Difosfoglicéricos/metabolismo , Ácidos Difosfoglicéricos/efeitos da radiação , Glutationa/metabolismo , Glutationa/efeitos da radiação , Técnicas In Vitro , Fosfolipídeos/metabolismo , Fosfolipídeos/efeitos da radiação , Doses de Radiação , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The effect of total body irradiation (5 Gy) on functional mouse erythroid lineage has been studied. The transferrin binding capacity by bone marrow cells and the activity of glycolytic regulatory enzymes and intracellular levels of 2,3 bisphosphoglycerate in peripheral blood erythrocytes have been determined. Results obtained along one year post-irradiation period suggest a complete recovery in the erythroid cell lineage with respect to the biological endpoints investigated.
Assuntos
Medula Óssea/efeitos da radiação , Ácidos Difosfoglicéricos/efeitos da radiação , Eritrócitos/efeitos da radiação , Fosfotransferases/efeitos da radiação , Receptores da Transferrina/efeitos da radiação , Transferrina/metabolismo , 2,3-Difosfoglicerato , Animais , Células da Medula Óssea , Ácidos Difosfoglicéricos/sangue , Eritrócitos/enzimologia , Hexoquinase/efeitos da radiação , Camundongos , Camundongos Endogâmicos BALB C , Fosfofrutoquinase-1/efeitos da radiação , Fosfotransferases/sangue , Piruvato Quinase/efeitos da radiação , Irradiação Corporal TotalRESUMO
The authors studied the effects of gamma radiation and submaximal physical exercise on 2,3-DFG content in healthy men erythrocytes. Twelve men aged 20-22 were examined. They were loaded by physical exercise (at doses of 2 M/kg body weight) for 15 minutes. Erythrocytes were exposed to gamma radiation (500 Gy doses) from a 60Co source. The concentration of 2,3-DFG in erythrocytes was estimated by Bartlett's method. Gamma radiation at 500 Gy dose was found to decrease 2,3-DFG content in erythrocytes both at rest and after submaximal exercise. Furthermore, submaximal physical exercise was found to decrease 2,3-DFG content in non-irradiated erythrocytes.