RESUMO
Transient implantable piezoelectric materials are desirable for biosensing, drug delivery, tissue regeneration, and antimicrobial and tumor therapy. For use in the human body, they must show flexibility, biocompatibility, and biodegradability. These requirements are challenging for conventional inorganic piezoelectric oxides and piezoelectric polymers. We discovered high piezoelectricity in a molecular crystal HOCH2(CF2)3CH2OH [2,2,3,3,4,4-hexafluoropentane-1,5-diol (HFPD)] with a large piezoelectric coefficient d33 of ~138 picocoulombs per newton and piezoelectric voltage constant g33 of ~2450 × 10-3 volt-meters per newton under no poling conditions, which also exhibits good biocompatibility toward biological cells and desirable biodegradation and biosafety in physiological environments. HFPD can be composite with polyvinyl alcohol to form flexible piezoelectric films with a d33 of 34.3 picocoulombs per newton. Our material demonstrates the ability for molecular crystals to have attractive piezoelectric properties and should be of interest for applications in transient implantable electromechanical devices.
Assuntos
Materiais Biocompatíveis , Compostos Férricos , Polímeros , Biodegradação Ambiental , Polímeros/química , Polímeros/metabolismo , Álcool de Polivinil/química , Álcool de Polivinil/metabolismo , Materiais Biocompatíveis/química , Materiais Biocompatíveis/metabolismo , Eletricidade , Animais , Ratos , Ratos Sprague-Dawley , Compostos Férricos/química , Compostos Férricos/metabolismoRESUMO
The development of cation electrokinetic concentrators (CECs) has been hindered by the lack of commercial anion-exchange membranes (AEMs). This paper introduces a γ-cyclodextrin-modified quaternized chitosan/polyvinyl alcohol (γ-CD/QCS/PVA) composite as an AEM, which is combined with a microchip to fabricate a CEC. Remarkably, the CEC only concentrates cationic species, thereby overcoming the interference of the highly abundant, negatively charged serum albumin in the blood sample. P-Glycoprotein (P-gp) is recognized as an efflux transporter protein that influences the pharmacokinetics (PK) of various compounds. The CEC was used to evaluate the activity of P-gp by detecting the positively charged rhodamine 123 (Rho123 is a classical substrate of P-gp) with no interference from serum albumin in the serum sample. Using the CEC, the enrichment factor (EF) of Rho123 exceeded 105-fold under DC voltage application. The minimal sample consumption of the CEC (<10 µL) enables reduction of animal sacrifice in animal experiments. Here, the CEC has been applied to evaluate the transport activity of P-gp in in vitro and in vivo experiments by detecting Rho123 in the presence of P-gp inhibitors or agonists. The results are in good agreement with those reported in previous reports. Therefore, the CEC presents a promising application potential, owing to its simple fabrication process, high sensitivity, minimal sample consumption, lack of interference from serum albumin and low cost.
Assuntos
Quitosana , gama-Ciclodextrinas , Animais , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Quitosana/química , Álcool de Polivinil/química , Álcool de Polivinil/metabolismo , Rodamina 123/farmacocinéticaRESUMO
This study aimed to verify the effects of polyvinyl alcohol (PVA) and bovine serum albumin (BSA) on the induction of full-type hyperactivation in boar spermatozoa treated with a cyclic AMP analog (cBiMPS). Washed spermatozoa were treated with cBiMPS (100 µM) for 180 min. As shown in the assessment of sperm motility, PVA (0.05%-0.4%) significantly promoted the induction of full-type hyperactivation, whereas BSA (0.025%-0.4%) did not affect the induction. In comparative experiments, BSA (0.4%) effectively promoted the induction of full-type hyperactivation in bovine spermatozoa treated with cBiMPS, calyculin A (a protein phosphatase inhibitor), and digoxin (a Na+ /K+ -ATPase inhibitor), while PVA (0.1%) did not affect the induction. Western blotting showed that protein tyrosine phosphorylation states of >50 kDa sperm proteins were effectively enhanced by treatment with cBiMPS in the PVA/BSA-free medium and not affected by the addition of PVA (0.1%). The assessment of plasma membrane integrity indicated that BSA (0.4%) significantly decreased spermatozoa with intact plasma membranes. These results indicate that PVA (0.1%) promotes the induction of full-type hyperactivation and does not influence the protein tyrosine phosphorylation states in boar cBiMPS-treated spermatozoa. They also suggest that BSA should not be added to medium containing cBiMPS for boar spermatozoa.
Assuntos
AMP Cíclico , Motilidade dos Espermatozoides , Suínos , Masculino , Animais , AMP Cíclico/farmacologia , Álcool de Polivinil/farmacologia , Álcool de Polivinil/metabolismo , Soroalbumina Bovina/farmacologia , Soroalbumina Bovina/metabolismo , Sêmen/metabolismo , Espermatozoides/fisiologia , Tirosina/metabolismoRESUMO
Transforming growth factor-beta 1 (TGF-ß1) has been reported to promote chondrogenic differentiation and proliferation in the multipotent stromal cell (MSCs), and the transforming growth factor-beta 3 (TGF-ß3) tends to be exclusively in promoting cell differentiation alone. The objective of this study was to determine the effect of TGF-ß1 and -ß3 on the MSCs chondrogenic differentiation on the poly (vinyl alcohol)-chitosan-poly (ethylene glycol) (PVA-NOCC-PEG) scaffold, compared with that of monolayer and pellet cultures. In this study, P2 rabbit bone marrow-derived MSCs were seeded either on the untreated six-well plate (for monolayer culture) or onto the PVA-NOCC-PEG scaffold or cultured as a pellet culture. The cultures were maintained in a chemically defined serum-free medium supplemented with 10 ng/mL of either TGF-ß1 or TGF-ß3. Cell viability assay, biochemical assay, and real-time polymerase chain reaction were performed to determine the net effect of cell proliferation and chondrogenic differentiation of each of the growth factors. The results showed that the PVA-NOCC-PEG scaffold enhanced MSCs cell proliferation from day 12 to 30 (p < 0.05); however, no significant differences were observed in the cell proliferation between the cultures supplemented with or without TGF-ß1 and TGF-ß3 (p > 0.05). In terms of chondrogenic differentiation, the PVA-NOCC-PEG scaffold augmented the GAGs secretion in MSCs and the mRNA expression levels of Sox9, Col2a1, Acan, and Comp were elevated (p < 0.05). However, there was no significant difference between both the TGF-ß1 and TGF-ß3-treated groups (p > 0.05). In conclusion, TGF-ß1 and TGF-ß3 enhanced the chondrogenic differentiation of MSCs seeded on the PVA-NOCC-PEG scaffold; however, there was no significant difference between the effect of TGF-ß1 and TGF-ß3. Impact statement Transforming growth factor-beta (TGF-ß) superfamily members is a key requirement for the in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs). In this study, the effects of TGF-ß1 and -ß3 on MSC chondrogenic differentiation and proliferation on a novel three-dimensional scaffold, the poly(vinyl alcohol)-chitosan-poly(ethylene glycol) (PVA-NOCC-PEG) scaffold, was evaluated. In this study, the results showed both TGF-ß1 and TGF-ß3 can enhance the chondrogenic differentiation of MSCs seeded on the PVA-NOCC-PEG scaffold.
Assuntos
Quitosana , Células-Tronco Mesenquimais , Animais , Coelhos , Fator de Crescimento Transformador beta3/metabolismo , Fator de Crescimento Transformador beta3/farmacologia , Álcool de Polivinil/farmacologia , Álcool de Polivinil/metabolismo , Quitosana/farmacologia , Quitosana/metabolismo , Fator de Crescimento Transformador beta1/farmacologia , Polietilenoglicóis/farmacologia , Condrogênese , Diferenciação Celular , Fator de Crescimento Transformador beta/farmacologia , Fatores de Crescimento Transformadores/metabolismo , Fatores de Crescimento Transformadores/farmacologia , Células CultivadasRESUMO
Chryseobacterium spp. are Gram-negative rods found ubiquitously in the environment, with certain species being reported as having unusual degrading properties. Polyvinyl alcohol (PVA) is used widely in industry but causes serious global environmental pollution. Here, we report the complete genome sequence of a novel bacterium, AC3, that efficiently degrades PVA. As the representative genome of Chryseobacterium nepalense, key genomic characteristics (e.g., mobile genetic elements, horizontal genes, genome-scale metabolic network, secondary metabolite biosynthesis gene clusters, and carbohydrate-active enzymes) were comprehensively investigated to reveal the potential genetic features of this species. Core genome phylogenetic analysis in combination with average nucleotide identity, average amino acid identity, and in silico DNA-DNA hybridization values provided an accurate taxonomic position of C. nepalense in the genus Chryseobacterium. Comparative genomic analysis of AC3 with closely related species suggested evolutionary dynamics characterized by a species-specific genetic repertoire, dramatic rearrangements, and evolutionary constraints driven by selective pressure, which facilitated the speciation and adaptative evolution of C. nepalense. Biogeographic characterization indicated that this species is ubiquitously distributed not only in soil habitats but also in a variety of other source niches. Bioinformatic analysis revealed the potential genetic basis of PVA degradation in AC3, which included six putative genes associated with the synthesis of PVA dehydrogenase, cytochrome c, oxidized PVA hydrolase, and secondary alcohol dehydrogenase. Our study reports the first complete genome of C. nepalense with PVA-degrading properties, providing comprehensive insights into the genomic characteristics of this species and increasing our understanding of the microbial degradation of PVA. IMPORTANCE Although PVA is a biodegradable polymer, the widespread use of PVA in global industrialization has resulted in serious environmental problems. To date, knowledge of effective and applicable PVA-degrading bacteria is limited, and thus, the discovery of novel PVA biodegraders is pertinent. Here, we isolated a novel bacterial strain, AC3, which efficiently degraded PVA. The complete genome of AC3 was sequenced as the first genome sequence of the species C. nepalense. Comparative genomic analysis was performed to comprehensively investigate the phylogenetic relationships, genome-scale metabolic network, key genomic characteristics associated with genomic evolution, evolutionary dynamics between AC3 and its close relatives, and biogeographic characterization of C. nepalense, particularly regarding the potential genetic basis of PVA degradation. These findings could advance our understanding of the genomic characteristics of C. nepalense and PVA bioremediation.
Assuntos
Chryseobacterium , Chryseobacterium/genética , Álcool de Polivinil/química , Álcool de Polivinil/metabolismo , Filogenia , Citocromos c/genética , Genômica , Bactérias/genética , Solo , Nucleotídeos , Aminoácidos , Carboidratos , Oxirredutases , DNA , RNA Ribossômico 16S/genéticaRESUMO
Owing to the noticeable increase in the number of patients with impaired wound healing capabilities, developing bioactive wound dressings with supportive physicomechanical and biological properties for clinical wound management has attracted much more attention nowadays. In this regard, engineered dressings with angiogenesis potential are vital for accelerated tissue regeneration. In the current study, nanoniosomal deferoxamine (DFO)-loaded transparent films of egg white-poly(vinyl alcohol) (PVA/EW/ND) were successfully fabricated at three different PVA/EW ratios (1:0, 1:1, and 1:1.5 wt/wt %) through the thin film hydration and solvent casting methods. The developed films' characterizations were carried out using scanning electron microscopy, Fourier transform infrared spectroscopy analysis, uniaxial tensile strength, water uptake, water vapor transmission rate, in vitro degradation, and drug release. The results demonstrated that the various weight ratios of PVA/EW have a significant effect on the microscopic morphology, equilibrium swelling, degradation, and mechanical properties of the films. The drug release profile exhibited a sustained release of DFO with controlled burst-lag phases resembling the Korsmeyer-Peppas pattern. The cytotoxicity and adhesion analysis using human dermal fibroblasts displays the biocompatibility of the developed PVA/EW/ND films and the formation of cellular colonies on the surface. The in vitro angiogenic capability of the developed films evaluated by the scratch wound assay and microbead-assisted tube formation study showed a significant increase in the rate of migration of human umbilical vein endothelial cells and in the number of tube-like structures. Therefore, the achieved results suggest that the presented PVA/EW/ND film has promising potential for effective wound healing applications.
Assuntos
Desferroxamina , Álcool de Polivinil , Bandagens , Desferroxamina/farmacologia , Clara de Ovo , Células Endoteliais/metabolismo , Humanos , Álcool de Polivinil/química , Álcool de Polivinil/metabolismo , Álcool de Polivinil/farmacologiaRESUMO
BACKGROUND: Annexin V, a member of calcium-dependent phospholipid-binding proteins, selectively binds to the exposed phosphatidylserine, which can be used for in vitro apoptosis detection. Simultaneous staining of cells with annexin V-fluorescein isothiocyanate (FITC) and the non-vital dye propidium iodide (PI) enables the detection of apoptotic and necrotic cells. OBJECTIVE: Our study aimed to express, purify, and stabilize the recombinant annexin V. METHODS: The recombinant annexin V was cloned and expressed in E. coli bacteria and was purified using Ni-IDA resin. The FITC conjugation was performed, and apoptosis detection of HaCaT cells by FITC-labeled annexin V was evaluated by flow cytometry. Then, the stability of FITC-labeled annexin in various conditions, including polyvinyl alcohol (PVA), glycerol, and trehalose, was evaluated. RESULTS: The results showed that annexin V was appropriately expressed and purified. After FITC conjugation, it could perfectly detect the cell death of HaCat cells in different apoptosis percentages. FITC-labeled annexin had more stability with PVA than glycerol and trehalose. CONCLUSION: It seems that PVA has an acceptable effect on FITC-labeled annexin V stability in concentrations lower than 1 mg mL-1 without interfering with fluorescent intensity.
Assuntos
Álcool de Polivinil , Trealose , Anexina A5/metabolismo , Fluoresceína , Fluoresceína-5-Isotiocianato , Álcool de Polivinil/metabolismo , Glicerol , Escherichia coli/genética , Escherichia coli/metabolismo , Citometria de Fluxo/métodos , ApoptoseRESUMO
Polymeric hydrogels are increasingly considered as scaffolds for tissue engineering due to their extraordinary resemblance with the extracellular matrix (ECM) of many tissues. As cell adhesion is a key factor in regulating important cell functions, hydrogel scaffolds are often functionalized or loaded with a variety of bioactive molecules that can promote adhesion. Interesting biomimetic approaches exploit the properties of mussel-inspired recombinant adhesive proteins. In this work, we prepared hydrogel scaffolds with a 50%w mixture of k-carrageenan (kC) and polyvinyl alcohol (PVA), by a two-step physical gelation process, and we coated them with Perna viridis foot protein-5ß (Pvfp5ß). The mechanical and morphological properties of hydrogels were investigated both after conditioning with typical cell culture media and also after coating with the Pvfp5ß. The protein resulted strongly adsorbed onto the surface of the hydrogel and also able to penetrate in its interiors to a certain depth, mainly interacting with the kC component of the scaffold as resulted from the confocal analysis. Mouse embryonic fibroblasts NIH-3T3 were seeded on top of the hydrogels and cultured up to two weeks. The role of Pvfp5ß in promoting cell adhesion, spreading and colonization of the scaffold was demonstrated.
Assuntos
Fibroblastos , Álcool de Polivinil , Animais , Carragenina/metabolismo , Adesão Celular/fisiologia , Fibroblastos/metabolismo , Hidrogéis/metabolismo , Hidrogéis/farmacologia , Camundongos , Álcool de Polivinil/metabolismo , Proteínas Recombinantes/metabolismo , Engenharia Tecidual/métodos , Alicerces TeciduaisRESUMO
BACKGROUND: Nerve tissues are important in coordinating the motions and movements of the body. Nerve tissue repair and regeneration is a slow process that might take a long time and cost a lot of money. As a result, tissue engineering was employed to treat nerve tissue lesions. The aim of this study was to investigate the proliferation of C6 cells and human mesenchymal stem cells derived bone marrow (hBMMSCs) differentiate into neuronal-like cells on the polyvinyl alcohol/gelatin/crocin (PVA/Gel/Cro) nanofiber scaffolds in vitro. METHODS: PVA/Gel scaffolds containing crocin in three concentrations (1%, 3%, and 5%) were prepared by the electrospinning method. The human bone marrow-derived mesenchymal stem cells (hBMSCs) differentiation on the PVA/Gel/Cro 5% that induced by beta-carotene (ßC), was analyzed during 10 days. Morphology of differentiated cells on the scaffolds was taken by scanning electron microscope (SEM). The expression of the neural cell markers was studied by quantitative reverse transcription- polymerase chain reaction (qRT-PCR) and immunocytochemistry (ICC). RESULTS: MTT results of C6 cells culture on the scaffolds showed that proliferation and metabolic activity on PVA/Gel scaffold containing crocin 5% (PVA/Gel/Cro 5%) are significantly more than the other concentrations (P = 0.01). MSC differentiation to nerve-like cells was approved by MAP-2 expression at the mRNA level and NESTIN and MAP-2 at the protein level. CONCLUSIONS: These results suggested that PVA/Gel/Cro 5% and ßC could lead to hBMSCs differentiation to neural cells.
Assuntos
Células-Tronco Mesenquimais , Álcool de Polivinil , Carotenoides , Diferenciação Celular , Gelatina/metabolismo , Gelatina/farmacologia , Humanos , Células-Tronco Mesenquimais/metabolismo , Álcool de Polivinil/metabolismo , Álcool de Polivinil/farmacologia , Engenharia Tecidual/métodos , Alicerces Teciduais , beta Caroteno/metabolismo , beta Caroteno/farmacologiaRESUMO
The attachment of bio-foulants, including unwanted cells, proteins, and bacteria, to a medical device such as an intraocular lens can lead to implantation failure. Hydrophilic polymers are often used as surface modifiers in the fabrication of anti-biofouling coatings, but a hydrophilic coating can easily become swollen and peel off the substrate. In this study, we chose polymethyl methacrylate (PMMA) as the representative material of intraocular lenses because PMMA has better biocompatibility, a higher refractive index, better optical clarity, lighter weight, more stable performance, and lower cost than other intraocular lens materials. We fabricated polyvinyl alcohol (PVA) coatings with or without a "bridge", that is, an intermediate adhesive layer (AL), to increase the adhesion bonding effect between the anti-biofouling coating and the substrate. The results indicated that the prepared coatings were transparent and noncytotoxic. Moreover, the anti-adhesion properties of the cells and the resistance properties to nonspecific protein adsorption of PMMA modified by both AL and PVA coatings were better and more durable compared with the sample only modified with a physically dipped PVA coating. The coating prepared by AL "bridging" provides a new strategy for the preparation of a transparent hydrophilic anti-biofouling coating suitable for PMMA intraocular lens materials.
Assuntos
Adesivos/química , Antibacterianos/química , Incrustação Biológica/prevenção & controle , Materiais Revestidos Biocompatíveis/química , Polimetil Metacrilato/química , Álcool de Polivinil/química , Adesivos/metabolismo , Adsorção , Antibacterianos/farmacologia , Biofilmes , Linhagem Celular , Materiais Revestidos Biocompatíveis/metabolismo , Humanos , Interações Hidrofóbicas e Hidrofílicas , Lentes Intraoculares , Teste de Materiais , Polimetil Metacrilato/metabolismo , Álcool de Polivinil/metabolismo , Propriedades de SuperfícieRESUMO
Oral cancer is a multifactorial cancer that affects millions of peoples worldwide. The current exploration aimed to evaluate the mechanisms that thymoquinone nanoencapsulated carrier and its effects on 7,12-Dimethylbenz[a]anthracene (DMBA) stimulated hamster buccal pouch cancer in Syrian hamster model. Nanocarrier was characterized by SEM, TEM, FTIR analysis. The incidence of tumor, and biochemicals makers was studied through standard methods. The mRNA expression level of inflammatory markers NF-κBp50, NF-κBp65, and PI3K/AKT/mTOR markers in the buccal tissues of control and experimental animals were investigated through RT-PCR analysis. In thymoquinone (TQ) loaded calcium alginate and polyvinyl alcohol carrier (TQ/Ca-alg-PVA) no squamous cell carcinogenesis developed and others moderate dysplasia revealed differentiated form of hyperplasia and keratosis. In biochemical analyses with DMBA + TQ/Ca-alg-PVA (20 mg/kg bw) orally administered hamsters showed restored the antioxidants, detoxification, xenobiotic metabolising enzymes in DMBA induced plasma and oral tissues of hamsters. Further, mRNA expression level of NF-κBp50/p65 and PI3K/AKT/mTOR were upregulated in the DMBA alone painted hamster. In contrast, these expressions were down regulated in orally TQ/Ca-alg-PVA treated experimental animals. This ability more eligible to deregulate the inflammatory and PI3K/AKT/mTOR signaling pathway that proved it suppresses anti-invasion/metastasis activity during hamster buccal pouch carcinogenesis. From this study, we recommended that TQ/Ca-alg-PVA has documented as effective chemopreventive agents, in further many molecular machineries need to study.
Assuntos
9,10-Dimetil-1,2-benzantraceno/toxicidade , Benzoquinonas/farmacologia , Álcool de Polivinil/farmacologia , 9,10-Dimetil-1,2-benzantraceno/efeitos adversos , Alginatos/efeitos adversos , Animais , Antracenos/efeitos adversos , Anticarcinógenos/farmacologia , Carcinogênese/efeitos dos fármacos , Carcinógenos , Carcinoma de Células Escamosas/patologia , Bochecha/patologia , Cricetinae , Regulação para Baixo/efeitos dos fármacos , Masculino , Mesocricetus , Neoplasias Bucais/patologia , Fosfatidilinositol 3-Quinases/metabolismo , Álcool de Polivinil/metabolismo , Álcool de Polivinil/uso terapêutico , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais/efeitos dos fármacos , Serina-Treonina Quinases TOR/metabolismoRESUMO
This study aimed to develop polyvinyl alcohol (PVA) -based scaffold enriched with hyaluronic acid (HA) and hydroxyapatite (HAp) using physical crosslinking by freezing-thawing method. We accomplished biological evaluation of scaffolds, swelling degree, bioactivity assessment, and hemolytic test. The results showed that all types of scaffolds should be safe for use in the human body. The culturing of human osteoblast-like cells MG-63 and their proliferation showed better adhesion of cells due to the presence of HA and confirmed better proliferation depending on the amount of HAp. This paper gives the optimal composition of the scaffold and the optimal amount of the particular components of the scaffold. Based on our results we concluded that the best PVA/HA/HAp combination is in the ratio 3:1:2.
Assuntos
Materiais Biocompatíveis/metabolismo , Durapatita/metabolismo , Ácido Hialurônico/metabolismo , Osteoblastos/metabolismo , Álcool de Polivinil/metabolismo , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Materiais Biocompatíveis/farmacologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Durapatita/farmacologia , Humanos , Ácido Hialurônico/farmacologia , Hidrogéis/química , Teste de Materiais/métodos , Álcool de Polivinil/farmacologiaRESUMO
In this study, we developed ticagrelor-dispersed nanosuspension (TCG-NSP) to enhance the dissolution and oral bioavailability of ticagrelor (TCG) through a statistical design approach. TCG, a reversible P2Y12 receptor antagonist, is classified as a biopharmaceutics classification system (BCS) class IV drug with low solubility and permeability, resulting in low oral bioavailability. Nanosuspension (NSP) is an efficient pharmaceutical technique for overcoming the disadvantages. First, we optimized TCG-NSP consisting of D-α-Tocopherol polyethylene glycol 1000 succinate (TPGS) and polyvinyl alcohol (PVA), which exhibited homogeneously dispersed TCG particle (233 nm) and low precipitation (3%). Characterization studies demonstrated that TCG-NSP provided amorphous TCG particles and supersaturation effect, resulting in higher dissolution than a commercial product. In addition, everted gut sac and pharmacokinetic studies confirmed that TCG-NSP improved the gastrointestinal permeation of TCG by 2.8-fold compared to commercial product, thereby enhancing the oral bioavailability (2.2-fold). These results suggested that TCG-NSP could be successfully used as an efficient pharmaceutical formulation to achieve the enhanced dissolution and oral bioavailability of TCG.
Assuntos
Sistemas de Liberação de Medicamentos/métodos , Nanopartículas/metabolismo , Álcool de Polivinil/metabolismo , Ticagrelor/metabolismo , Vitamina E/metabolismo , Administração Oral , Animais , Disponibilidade Biológica , Células CACO-2 , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Relação Dose-Resposta a Droga , Liberação Controlada de Fármacos/efeitos dos fármacos , Liberação Controlada de Fármacos/fisiologia , Humanos , Masculino , Nanopartículas/administração & dosagem , Nanopartículas/química , Técnicas de Cultura de Órgãos , Álcool de Polivinil/administração & dosagem , Álcool de Polivinil/química , Ratos , Ratos Sprague-Dawley , Solubilidade , Ticagrelor/administração & dosagem , Ticagrelor/química , Vitamina E/administração & dosagem , Vitamina E/químicaRESUMO
Polymeric biomaterials capable of delivering nitric oxide (NO) topically can be used to enhance skin blood flow (SkBF) and accelerate wound healing. Herein, we used reversible addition-fragmentation chain transfer radical (RAFT) polymerization to synthesize the first poly(vinyl alcohol) (PVA) functionalized with terminal NO-releasing S-nitrosothiol (RSNO) groups for topical NO delivery. This strategy was based on the synthesis of a precursor amino-terminated PVA (PVA-NH2), which was next functionalized with iminothiolane yielding 4-imino-4-amino-PVA-butane-1-thiol (PVA-SH), and finally S-nitrosated yielding S-nitroso 4-imino-4-amino-PVA-butane-1-thiol (PVA-SNO). Real-time chemiluminescence NO detection showed that blended films of pure PVA with PVA-SNO with mass ratios 30:70, 50:50 and 70:30 release NO with initial rates ranging from 1 to 12 nmol g-1 min-1, and lead to a 2 to 10-fold dose-response increase in the SkBF, after topical application on the ventral forearm of volunteers. These results show that PVA-SNO is a potential platform for topical NO delivery in biomedical applications.
Assuntos
Óxido Nítrico/metabolismo , Álcool de Polivinil/metabolismo , S-Nitrosoglutationa/metabolismo , Pele/metabolismo , Velocidade do Fluxo Sanguíneo , Humanos , Pele/irrigação sanguíneaRESUMO
The interest in developing new materials intended for food packaging based on bacterial cellulose is growing in the recent years. Flexible and transparent films from bacterial cellulose-chitosan-polyvinyl alcohol have shown excellent UV-barrier properties. However, this material interacts with ambient moisture modifying its water activity due to its hydrophilic nature. In this work, a study was carried out to evaluate the changes caused by the water activity. Results showed a plasticizing effect of water molecules increasing the water vapor permeability of the samples from 1.86 × 10-12 to 1.17 × 10-11 g/m·s·Pa, percentage of elongation from 3.25 to 36.55%, and distance to burst from 0.64 to 5.12 mm. The increase of the water activity decreased the Young's modulus and tensile strength. The values of the UV-barrier were maintained at the wide range of water activity. Consequently, water molecules do not affect the UV-barrier properties of the films.
Assuntos
Celulose/química , Quitosana/química , Química Verde/métodos , Álcool de Polivinil/química , Vapor , Estresse Mecânico , Acetobacteraceae/metabolismo , Celulose/metabolismo , Quitosana/metabolismo , Módulo de Elasticidade , Embalagem de Alimentos/métodos , Álcool de Polivinil/metabolismo , Resistência à Tração/fisiologiaRESUMO
The combination of high mechanical properties, antibacterial activity and a green synthesis of the polyvinyl alcohol (PVA) based films remains challenging. This study presents a ternary system of PVA films containing bacterial cellulose (BC) and epsilon-polylysine (ε-PL) by a green solution casting method. The prepared composite films showed more than 99% antibacterial properties against both Staphylococcus aureus and Escherichia coli bacteria. Moreover, the films were collected after a single use and were reused twice, which still exhibited strong antibacterial activity. The films showed thermal stability and higher mechanical properties as compared to pure PVA films. In addition, the cytotoxicity of the films was evaluated by MTT assay against NIH 3T3 mouse fibroblast cells. The results showed no toxicity of the films towards tested cells. We believe that these antibacterial films may find applications in active food packaging and biomedical fields.
Assuntos
Antibacterianos/química , Bactérias/metabolismo , Celulose/química , Polilisina/química , Álcool de Polivinil/química , Animais , Antibacterianos/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Celulose/metabolismo , Estabilidade de Medicamentos , Escherichia coli/efeitos dos fármacos , Fenômenos Mecânicos , Camundongos , Testes de Sensibilidade Microbiana , Células NIH 3T3 , Polilisina/metabolismo , Álcool de Polivinil/metabolismo , Staphylococcus aureus/efeitos dos fármacos , TemperaturaRESUMO
The morphology controlled molecular assemblies play vital roles in biological systems. Here we present endogenous reactive oxygen species (ROS)-triggered morphology transformation of polymer-peptide conjugates (PPCs) for cooperative interaction with mitochondria, exhibiting high tumor therapeutic efficacy. The PPCs are composed of (i) a ß-sheet-forming peptide KLVFF conjugated with poly(ethylene glycol) through ROS-cleavable thioketal, (ii) a mitochondria-targeting cytotoxic peptide KLAK, and (iii) a poly(vinyl alcohol) backbone. The self-assembled PPCs nanoparticles can enter cells and target mitochondria. Because of overgenerated ROS around mitochondria in most cancer cells, the thioketal linker can be cleaved, leading to transformation from nanoparticles to fibrous nanostructures. As a result, the locational nanofibers with exposure of KLAK exhibit enhanced multivalent cooperative interactions with mitochondria, which causes selective cytotoxicity against cancer cells and powerful tumor suppression efficacy in vivo. As the first example of ROS-triggered intracellular transformation, the locational assembly strategy in vivo may provide a new insight for disease diagnosis and therapy through enhanced interaction with targeting site.
Assuntos
Antineoplásicos/metabolismo , Mitocôndrias/metabolismo , Peptídeos/metabolismo , Álcool de Polivinil/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Animais , Antineoplásicos/química , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Camundongos , Mitocôndrias/química , Neoplasias Experimentais/diagnóstico por imagem , Neoplasias Experimentais/tratamento farmacológico , Imagem Óptica , Peptídeos/química , Peptídeos/farmacologia , Álcool de Polivinil/química , Álcool de Polivinil/farmacologia , Espécies Reativas de Oxigênio/químicaRESUMO
To discuss the microbial structure that develops during the course of compost burials of polyvinyl alcohol (PVA)-based material and analyze the effect of the degree of PVA alcoholysis on the PVA-degrading ability of each strain. Two bacterial strains exhibiting a high level of PVA-degrading ability and two mixed cultures were isolated from compost loaded with PVA-based material. The rod-shaped Gram-positive bacterium DG22 was identified by 16S rDNA sequencing analysis as Bacillus sp. DG22, and the rod-shaped Gram-negative bacterium DG14 was identified as Paenibacillus sp. DG14. Biodegradation rates were determined by iodometric analysis within 8 days of culturing on selective media. The biodegradation rates for PVA1799 by DG22, DG14, T1 (mixed culture from a 36-month burial), and T2 (mixed culture from a 24-month burial) were 54.47, 46.59, 69.62, and 79.04%, respectively, and those of PVA1788 were 74.99, 67.27, 54.43, and 50.76%, respectively. DG22 and DG14 are the first known Bacillus sp. and Paenibacillus sp. able to utilize PVA solely. However, the higher degree of PVA alcoholysis (AD) had a negative effect on the biodegradation of PVA.
Assuntos
Bactérias/metabolismo , Compostagem , Álcool de Polivinil/metabolismo , Bacillus/isolamento & purificação , Bacillus/metabolismo , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Biodegradação Ambiental , Paenibacillus/isolamento & purificação , Paenibacillus/metabolismo , Filogenia , RNA Ribossômico 16S/genéticaRESUMO
In this study, polyvinyl alcohol (PVA)-degrading bacteria were screened from sludge samples using PVA as a sole source of carbon. A novel strain was obtained and identified as Bacillus niacini based on the analysis of a partial 16S rDNA nucleotide sequence and morphological characteristics. PVA-degrading enzyme (PVAase) from Bacillus niacini was immobilized as cross-linked enzyme aggregates (CLEAs) via precipitation with ammonium sulfate followed by glutaraldehyde cross-linking. The effects of precipitation and cross-linking on PVAase-CLEAs activity were investigated and characterized. 70% ammonium sulfate and 1.5% glutaraldehyde were used for precipitation and 1-h cross-linking reaction. The activity recovery of PVAase-CLEAs was approximately 90% starting from free PVAase, suggesting non-purification steps are required for extended use. No significant differences in optimum pH and temperature values of the PVAase were recorded after immobilization. The PVAase-CLEAs showed a ball-like morphology and enhanced PVA degradation efficiency in comparison with the free PVAase in solution. Furthermore, the PVAase-CLEAs exhibited excellent thermal stability, pH stability and storage stability compared to free PVAase. The PVAase-CLEAs retained about 75% of initial PVAase activity after 4â¯cycles of use. These results suggest that this CLEA is potentially usable for PVA degradation in industrial applications.
Assuntos
Bacillus/enzimologia , Biodegradação Ambiental , Enzimas Imobilizadas/química , Álcool de Polivinil/química , Carbono/química , Reagentes de Ligações Cruzadas/química , Estabilidade Enzimática , Cinética , Álcool de Polivinil/metabolismo , Agregados Proteicos/genética , RNA Ribossômico 16S/genética , Esgotos/química , TemperaturaRESUMO
Slow-release fertilizer has been proven to be more effective than traditional fertilizer for providing a long-term stable nutrient supply. Although such fertilizers have been widely investigated, their water-retention properties and biodegradability have not been fully analysed. Composites of fertilizers and polymers provide opportunities to prepare new types of fertilizer with enhanced properties for real applications. Chicken feather protein-graft-poly(potassium acrylate)-polyvinyl alcohol semi-interpenetrating networks forming a super absorbent resin combined with nitrogen (N) and phosphorus (P) (CFP-g-PKA/PVA/NP semi-IPNs SAR) was prepared. The chemically bonded or physically embedded fertilizer compound could be released form the resin matrix to the surrounding soil under irrigation. The synthesis mechanism, morphology, and chemical and mechanical structure of the synthesized composites were investigated. The reactant doses were optimized through response surface methodology (RSM). A 30-day field trial of the prepared SAR was applied to detect the influence of sample particle size, soil salinity, pH, and moisture content on the slow-release behaviour of N and P. The maximum release values of N and P from the composites were 69.46% N and 65.23% P. A 120-day soil burying experiment and 30-day Aspergillus niger (A. niger) inoculation were performed, and the biodegradability and change in microstructure were monitored. The addition of SAR to soil could also improve the water-retention ability of the soil.