RESUMO
Radiation-induced skin injury remains a serious concern for cancer radiotherapy, radiation accidents and occupational exposure, and the damage mainly occurs due to apoptosis and reactive oxygen species (ROS) generation. There is currently no effective treatment for this disorder. The ß-catenin signaling pathway is involved in the repair and regeneration of injured tissues. However, the role of the ß-catenin signaling pathway in radiation-induced skin injury has not been reported. In this study, we demonstrated that the ß-catenin signaling pathway was activated in response to radiation and that its activation by Wnt3a, a ligand-protein involved in the ß-catenin signaling pathway, inhibited apoptosis and the production of ROS in irradiated human keratinocyte HaCaT cells and skin fibroblast WS1 cells. Additionally, Wnt3a promoted cell migration after irradiation. In a mouse model of full-thickness skin wounds combined with total-body irradiation, Wnt3a was shown to facilitate skin wound healing. The results from RNA-Seq revealed that 24 genes were upregulated and 154 were downregulated in Wnt3a-treated irradiated skin cells, and these dysregulated genes were mainly enriched in the tight junction pathway. Among them, Marvel D3 showed the most obvious difference. We further found that the activated ß-catenin signaling pathway stimulated the phosphorylation of JNK by silencing Marvel D3. Treatment of irradiated cells with SP600125, a JNK inhibitor, augmented ROS production and impeded cell migration. Furthermore, treatment with Wnt3a or transfection with Marvel D3-specific siRNAs could reverse the above effects. Taken together, these findings illustrate that activated ß-catenin signaling stimulates the activation of JNK by negatively regulating Marvel D3 to ameliorate radiation-induced skin injury.
Assuntos
Anormalidades Induzidas por Radiação/genética , MAP Quinase Quinase 4/genética , Via de Sinalização Wnt/genética , Proteína Wnt3A/genética , beta Catenina/genética , Anormalidades Induzidas por Radiação/tratamento farmacológico , Anormalidades Induzidas por Radiação/patologia , Animais , Antracenos/farmacologia , Apoptose/efeitos dos fármacos , Apoptose/genética , Movimento Celular/genética , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Queratinócitos/metabolismo , MAP Quinase Quinase 4/antagonistas & inibidores , Camundongos , Fosforilação/genética , RNA Interferente Pequeno/farmacologia , Espécies Reativas de Oxigênio , Via de Sinalização Wnt/efeitos da radiação , Cicatrização/genéticaRESUMO
Radiotherapy is a major modality used to combat a wide range of cancers. Classical radiobiology principles categorize ionizing radiation (IR) as a direct cytocidal therapeutic agent against cancer; however, there is an emerging appreciation for additional antitumor immune responses generated by this modality. A more nuanced understanding of the immunological pathways induced by radiation could inform optimal therapeutic combinations to harness radiation-induced antitumor immunity and improve treatment outcomes of cancers refractory to current radiotherapy regimens. Here, we summarize how radiation-induced DNA damage leads to the activation of a cytosolic DNA sensing pathway mediated by cyclic GMP-AMP (cGAMP) synthase (cGAS) and stimulator of interferon genes (STING). The activation of cGAS-STING initiates innate immune signaling that facilitates adaptive immune responses to destroy cancer. In this way, cGAS-STING signaling bridges the DNA damaging capacity of IR with the activation of CD8+ cytotoxic T cell-mediated destruction of cancer-highlighting a molecular pathway radiotherapy can exploit to induce antitumor immune responses. In the context of radiotherapy, we further report on factors that enhance or inhibit cGAS-STING signaling, deleterious effects associated with cGAS-STING activation, and promising therapeutic candidates being investigated in combination with IR to bolster immune activation through engaging STING-signaling. A clearer understanding of how IR activates cGAS-STING signaling will inform immune-based treatment strategies to maximize the antitumor efficacy of radiotherapy, improving therapeutic outcomes.
Assuntos
Anormalidades Induzidas por Radiação/genética , Imunidade/genética , Proteínas de Membrana/genética , Neoplasias/genética , Nucleotidiltransferases/genética , Anormalidades Induzidas por Radiação/imunologia , Anormalidades Induzidas por Radiação/patologia , Dano ao DNA/imunologia , Dano ao DNA/efeitos da radiação , Humanos , Imunidade/imunologia , Imunidade/efeitos da radiação , Imunidade Inata/efeitos da radiação , Proteínas de Membrana/imunologia , Neoplasias/imunologia , Neoplasias/patologia , Neoplasias/radioterapia , Nucleotidiltransferases/imunologia , Transdução de Sinais/efeitos da radiaçãoRESUMO
Radiation protection on male testis is an important task for ionizing radiation-related workers or people who receive radiotherapy for tumours near the testicle. In recent years, Toll-like receptors (TLRs), especially TLR4, have been widely studied as a radiation protection target. In this study, we detected that a low-toxicity TLR4 agonist monophosphoryl lipid A (MPLA) produced obvious radiation protection effects on mice testis. We found that MPLA effectively alleviated testis structure damage and cell apoptosis induced by ionizing radiation (IR). However, as the expression abundance differs a lot in distinct cells and tissues, MPLA seemed not to directly activate TLR4 singling pathway in mice testis. Here, we demonstrated a brand new mechanism for MPLA producing radiation protection effects on testis. We observed a significant activation of TLR4 pathway in macrophages after MPLA stimulation and identified significant changes in macrophage-derived exosomes protein expression. We proved that after MPLA treatment, macrophage-derived exosomes played an important role in testis radiation protection, and specially, G-CSF and MIP-2 in exosomes are the core molecules in this protection effect.
Assuntos
Anormalidades Induzidas por Radiação/genética , Lipídeo A/análogos & derivados , Testículo/lesões , Receptor 4 Toll-Like/genética , Anormalidades Induzidas por Radiação/tratamento farmacológico , Anormalidades Induzidas por Radiação/patologia , Animais , Modelos Animais de Doenças , Exossomos/efeitos dos fármacos , Humanos , Lipídeo A/química , Lipídeo A/genética , Lipídeo A/farmacologia , Masculino , Camundongos , Proteção Radiológica , Testículo/efeitos dos fármacos , Testículo/patologia , Testículo/efeitos da radiação , Receptor 4 Toll-Like/agonistasRESUMO
The functions and molecular mechanism of circRNAs in the development of radiation-induced liver disease (RILD) remain largely unknown. The goal of this study was to explore the expression and potential role of a new circular RNA, named circTUBD1, in irradiated and lipopolysaccharide (LPS)-stimulated human hepatic stellate cell (HSC) line LX-2 cells. The expression of circTUBD1 was significantly upregulated in irradiated and LPS-stimulated LX-2 cells compared to non-treated LX-2 cells. To explore the functions of circTUBD1, small interfering RNAs targeting circTUBD1 were designed. Silencing circTUBD1 inhibited proliferation, promoted apoptosis of LX-2 cells, and significantly decreased the expression level of pro-inflammatory cytokines, including IL-1ß, IL-6 and TNF-α in irradiated and LPS-stimulated LX-2 cells. Mechanistic analysis suggested that circTUBD1 acted as the miR-146a-5p sponge to affect pro-inflammatory cytokine production through regulating expression of Toll-like receptor 4 (TLR4), interleukin receptor-associated kinase 1 (IRAK1), tumor necrosis factor receptor-associated factor-6 (TRAF6), and phosphorylation of nuclear factor-kappa B (pNF-κB) in irradiated and LPS-stimulated LX-2 cells. To our knowledge, this is the first study to show that circTUBD1 acts as a miR-146a-5p sponge to affect the viability and pro-inflammatory cytokine production of LX-2 cells through the TLR4 pathway, suggesting that circTUBD1 is a potential target for RILD therapy.
Assuntos
Anormalidades Induzidas por Radiação/genética , MicroRNAs/genética , RNA Circular/genética , Receptor 4 Toll-Like/genética , Sobrevivência Celular/efeitos da radiação , Citocinas/biossíntese , Citocinas/genética , Células Estreladas do Fígado/patologia , Células Estreladas do Fígado/efeitos da radiação , Humanos , Fígado/metabolismo , Fígado/patologia , Fígado/efeitos da radiação , Hepatopatias/etiologia , Transdução de Sinais/efeitos da radiação , Tubulina (Proteína)/genéticaRESUMO
Radiation-induced lung injury (RILI) is one of the most common and fatal complications of thoracic radiotherapy, whereas no effective interventions are available. Andrographolide, an active component extracted from Andrographis paniculate, is prescribed as a treatment for upper respiratory tract infection. Here we report the potential radioprotective effect and mechanism of Andrographolide on RILI. C57BL/6 mice were exposed to 18 Gy of whole thorax irradiation, followed by intraperitoneal injection of Andrographolide every other day for 4 weeks. Andrographolide significantly ameliorated radiation-induced lung tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine release in the early phase and progressive fibrosis in the late phase. Moreover, Andrographolide markedly hampered radiation-induced activation of the AIM2 inflammasome and pyroptosis in vivo. Furthermore, bone marrow-derived macrophages (BMDMs) were exposed to 8 Gy of X-ray radiation in vitro and Andrographolide significantly inhibited AIM2 inflammasome mediated-pyroptosis in BMDMs. Mechanistically, Andrographolide effectively prevented AIM2 from translocating into the nucleus to sense DNA damage induced by radiation or chemotherapeutic agents in BMDMs. Taken together, Andrographolide ameliorates RILI by suppressing AIM2 inflammasome mediated-pyroptosis in macrophage, identifying Andrographolide as a novel potential protective agent for RILI.
Assuntos
Anormalidades Induzidas por Radiação/tratamento farmacológico , Proteínas de Ligação a DNA/genética , Diterpenos/farmacologia , Lesão Pulmonar/tratamento farmacológico , Anormalidades Induzidas por Radiação/genética , Anormalidades Induzidas por Radiação/patologia , Animais , Modelos Animais de Doenças , Humanos , Inflamassomos/efeitos dos fármacos , Inflamassomos/genética , Inflamação/tratamento farmacológico , Inflamação/etiologia , Inflamação/genética , Inflamação/patologia , Pulmão/anormalidades , Pulmão/efeitos dos fármacos , Pulmão/efeitos da radiação , Lesão Pulmonar/etiologia , Lesão Pulmonar/patologia , Camundongos , Piroptose/efeitos dos fármacos , Piroptose/genética , Protetores contra Radiação/farmacologiaRESUMO
Ehlers-Danlos syndrome (EDS) is a heritable connective tissue disorder characterized by skin hyperextensibility, abnormal wound healing, and joint hypermobility with prevalence 1:20â¯000. Its incidence is probably underestimated due to unknown number of subjects having mild symptoms who may have never been diagnosed through entire life time. Classical EDS is characterized by pathogenic variants of genes encoding type V collagen. The biological effects and health risks of patients with EDS exposure to low doses of ionizing radiation is poorly understood. The aim of this study was to investigate biological effect of low doses of ionizing radiation in children with EDS. Background values of chromosome aberrations in children suffering from classical EDS were determined and compared with control subjects. The in vitro experiment was performed by γ-irradiation of blood lymphocytes from EDS patients and healthy subjects at low doses (0.1, 0.2 and 0.3â¯Gy). Results show a significant increase level of spontaneous and radiation-induced chromosomal aberrations in children suffering from EDS in comparison with the control subjects (pâ¯<â¯0.05). In conclusion, children with EDS express higher background chromosome aberration frequency and increased radiosensitivity. These findings suggest specific susceptibility of EDS patients and importance of future investigation on risks of diagnostics and therapy which include radiation and genotoxic agents.
Assuntos
Anormalidades Induzidas por Radiação/genética , Aberrações Cromossômicas/efeitos da radiação , Síndrome de Ehlers-Danlos/genética , Anormalidades da Pele/genética , Adolescente , Criança , Pré-Escolar , Síndrome de Ehlers-Danlos/fisiopatologia , Feminino , Genoma Humano/efeitos da radiação , Humanos , Instabilidade Articular/etiologia , Instabilidade Articular/genética , Masculino , Doses de Radiação , Tolerância a Radiação/genética , Radiação Ionizante , Anormalidades da Pele/etiologiaRESUMO
AIM: In this study, we investigated expression levels of cyclooxygenase-2 (COX-2) and endothelin-1 (ET-1) genes after pelvis and heart irradiation in a rat model. These factors are involved in heart diseases (HDs). MATERIALS AND METHODS: We used seven groups, including two groups of pelvic irradiation, two groups of whole body irradiation, two groups of heart irradiation, and one control nonirradiated group. Pelvis irradiations were conducted at a 2 cm × 2 cm in the pelvis area. Irradiation condition conducted using 1.25 MeV cobalt-60 gamma-rays (30 cGy/min). The changes at ET-1 and COX-2 gene expressions in heart tissue after pelvis and heart irradiation were measured and compared to the control and whole body irradiation groups at 24 h and 72 h after the exposure. RESULTS: In heart irradiation groups, 3-fold up-regulation of both ET-1 and COX-2 was observed. In pelvis irradiation groups, 3-fold up-regulation of ET-1 was seen, but not significant changes in COX-2 gene expression have observed at distant heart tissues after pelvis irradiation. CONCLUSION: This study reveals that nontargeted effect induced by radiation may be considered as an important phenomenon for induction of HD after radiotherapy.
Assuntos
Anormalidades Induzidas por Radiação/genética , Ciclo-Oxigenase 2/genética , Endotelina-1/genética , Neoplasias/radioterapia , Animais , Regulação Neoplásica da Expressão Gênica , Coração/efeitos da radiação , Humanos , Miocárdio/metabolismo , Neoplasias/genética , Neoplasias/patologia , Pelve/patologia , Pelve/efeitos da radiação , RatosRESUMO
In experimental organisms such as fruit flies and mice, increased frequencies in germ cell mutations have been detected following exposure to ionizing radiation. In contrast, there has been no clear evidence for radiation-induced germ cell mutations in humans that lead to birth defects, chromosome aberrations, Mendelian disorders, etc. This situation exists partly because no sensitive and practical genetic marker is available for human studies and also because the number of people exposed to large doses of radiation and subsequently having offspring was small until childhood cancer survivors became an important study population. In addition, the genome of apparently normal individuals seems to contain large numbers of alterations, including dozens to hundreds of nonfunctional alleles. With the number of mutational events in protein-coding genes estimated as less than one per genome after 1 gray (Gy) exposure, it is unsurprising that genetic effects from radiation have not yet been detected conclusively in humans.
Assuntos
Genoma Humano/efeitos da radiação , Anormalidades Induzidas por Radiação/etiologia , Anormalidades Induzidas por Radiação/genética , Animais , Aberrações Cromossômicas , Cromossomos/efeitos da radiação , Dano ao DNA , Relação Dose-Resposta à Radiação , Drosophila melanogaster/efeitos da radiação , Feminino , Seguimentos , Mutação em Linhagem Germinativa/efeitos da radiação , Humanos , Masculino , Camundongos , Modelos Animais , Mutagênese , Neoplasias Induzidas por Radiação/epidemiologia , Neoplasias Induzidas por Radiação/genética , Armas Nucleares , Exposição Ocupacional , Lesões por Radiação/genética , Tolerância a Radiação , Liberação Nociva de Radioativos , Radioterapia/efeitos adversos , Razão de Masculinidade , SobreviventesRESUMO
There is no convincing evidence regarding radiation-induced heritable risks of adult-onset multifactorial diseases in humans, although it is important from the standpoint of protection and management of populations exposed to radiation. The objective of the present study was to examine whether parental exposure to atomic-bomb (A-bomb) radiation led to an increased risk of common polygenic, multifactorial diseases-hypertension, hypercholesterolaemia, diabetes mellitus, angina pectoris, myocardial infarction or stroke-in the first-generation (F1) offspring of A-bomb survivors. A total of 11,951 F1 offspring of survivors in Hiroshima or Nagasaki, conceived after the bombing, underwent health examinations to assess disease prevalence. We found no evidence that paternal or maternal A-bomb radiation dose, or the sum of their doses, was associated with an increased risk of any multifactorial diseases in either male or female offspring. None of the 18 radiation dose-response slopes, adjusted for other risk factors for the diseases, was statistically significantly elevated. However, the study population is still in mid-life (mean age 48.6 years), and will express much of its multifactorial disease incidence in the future, so ongoing longitudinal follow-up will provide increasingly informative risk estimates regarding hereditary genetic effects for incidence of adult-onset multifactorial disease.
Assuntos
Anormalidades Induzidas por Radiação/epidemiologia , Anormalidades Induzidas por Radiação/genética , Doenças Genéticas Inatas/epidemiologia , Exposição Materna/estatística & dados numéricos , Guerra Nuclear/estatística & dados numéricos , Exposição Paterna/estatística & dados numéricos , Sobreviventes/estatística & dados numéricos , Carga Corporal (Radioterapia) , Causalidade , Feminino , Humanos , Incidência , Japão/epidemiologia , Masculino , Pessoa de Meia-Idade , Monitoramento de Radiação/estatística & dados numéricos , Medição de RiscoRESUMO
Two Drosophila melanogaster strains (Canton-S and ri-lines) for 20 generations were in the controlled terms of chronic irradiation with 3-dose rate (1,2 x 10(-8); 0,3 x 10(-8); 0,12 x 10(-8) Gy/c). The dynamics of hybrid dysgenesis frequency was explored for each generation of F1 descendants from Canton-S and ri-lines crossing. The gradual change of dose response of hybrid dispense depending on duration of irradiation of ancestors and dose rate was shown. The complex dynamics of hybrid dysgenesis frequency depending on irradiation duration of ancestors and dose rate was detected. The cumulative effect of the prolonged irradiation shows up as adaptation at the lowest dose rate and as exhaustion at the highest dose rate. Question comes into discussion about the features of transitional process and including of protective and adaptive reactions hierarchy at the conditions of radiation factor chronic action.
Assuntos
Anormalidades Induzidas por Radiação/etiologia , Drosophila melanogaster/efeitos da radiação , Raios gama/efeitos adversos , Disgenesia Gonadal/etiologia , Anormalidades Induzidas por Radiação/genética , Adaptação Fisiológica/genética , Adaptação Fisiológica/efeitos da radiação , Animais , Cruzamentos Genéticos , Elementos de DNA Transponíveis/genética , Relação Dose-Resposta à Radiação , Drosophila melanogaster/genética , Drosophila melanogaster/crescimento & desenvolvimento , Feminino , Disgenesia Gonadal/genética , MasculinoRESUMO
Recent results have shown that irradiation of a single cell, the zygote or 1-cell embryo of various mouse strains, could lead to congenital anomalies in the fetuses. In the Heiligenberger strain, a link between the radiation-induced congenital anomalies and the development of a genomic instability was also suggested. Moreover, further studies showed that in that strain, both congenital anomalies and genomic instability could be transmitted to the next generation. The aim of the experiments described in this paper was to investigate whether such non-targeted transgenerational effects could also be observed in two other radiosensitive mouse strains (CF1 and ICR), using lower radiation doses. Irradiation of the CF1 and ICR female zygotes with 0.2 or 0.4Gy did not result in a decrease of their fertility after birth, when they had reached sexual maturity. Moreover, females of both strains that had been X-irradiated with 0.2Gy exhibited higher rates of pregnancy, less resorptions and more living fetuses. Additionally, the mean weight of living fetuses in these groups had significantly increased. Exencephaly and dwarfism were observed in CF1 fetuses issued from control and X-irradiated females. In the control group of that strain, polydactyly and limb deformity were also found. The yields of abnormal fetuses did not differ significantly between the control and X-irradiated groups. Polydactyly, exencephaly and dwarfism were observed in fetuses issued from ICR control females. In addition to these anomalies, gastroschisis, curly tail and open eye were observed at low frequencies in ICR fetuses issued from X-irradiated females. Again, the frequencies of abnormal fetuses found in the different groups did not differ significantly. In both CF1 and ICR mouse strains, irradiation of female zygotes did not result in the development of a genomic instability in the next generation embryos. Overall, our results suggest that, at the moderate doses used, developmental defects observed after X-irradiation of female zygotes of these two sensitive mouse strains should not be transmitted to the next generation. Paradoxically, other studies would be needed to address the question of a potential increase of fertility after doses lower than 0.2Gy in both strains.
Assuntos
Anormalidades Induzidas por Radiação/genética , Blastocisto/efeitos da radiação , Desenvolvimento Embrionário/efeitos da radiação , Animais , Embrião de Mamíferos/efeitos da radiação , Feminino , Instabilidade Genômica , Camundongos , Camundongos Endogâmicos ICR , GravidezRESUMO
Translocations have provided invaluable tools for identifying both cancer-linked genes and loci associated with heritable human diseases, but heritable human translocations are rare and few mouse models exist. Here we report progress on analysis of a collection of heritable translocations generated by treatment of mice with specific chemicals or radiation during late spermatogenic stages. The translocation mutants exhibit a range of visible phenotypes reflecting the disruption of coding sequences or the separation of genes from essential regulatory elements. The breakpoints of both radiation-induced and chemically induced mutations in these mice are remarkably clean, with very short deletions, duplications, or inversions in some cases, and ligation mediated by microhomology, suggesting nonhomologous end joining as the major path of repair. These mutations provide new tools for the discovery of novel genes and regulatory elements linked to human developmental disorders and new clues to the molecular basis of human genetic disease.
Assuntos
Anormalidades Induzidas por Radiação/genética , Doenças Genéticas Inatas/genética , Células Germinativas , Translocação Genética , Animais , Sequência de Bases , Aberrações Cromossômicas , Doenças Genéticas Inatas/induzido quimicamente , Humanos , Camundongos , Dados de Sequência Molecular , MutagêneseRESUMO
It is now well accepted that parental whole body irradiation causes transgenerational genome and epigenome instability in the offspring. The majority of human exposures to radiation, such as therapeutic and diagnostic irradiation, are localized and focused. The potential of localized body-part exposures to affect the germline and thus induce deleterious changes in the progeny has not been studied. To investigate whether or not the paternal cranial irradiation can exert deleterious changes in the protected germline, we studied the accumulation of DNA damage in the shielded testes tissue. Here we report that the localized paternal cranial irradiation results in a significant accumulation of unrepaired DNA lesions in sperm cells and leads to a profound epigenetic dysregulation in the unexposed progeny conceived a week after paternal exposure.
Assuntos
Anormalidades Induzidas por Radiação/metabolismo , Efeito Espectador/efeitos da radiação , Irradiação Craniana/efeitos adversos , Dano ao DNA/efeitos da radiação , Epigênese Genética/efeitos da radiação , Células Germinativas/efeitos da radiação , Anormalidades Induzidas por Radiação/genética , Anormalidades Induzidas por Radiação/fisiopatologia , Animais , Medula Óssea/patologia , Medula Óssea/fisiopatologia , Medula Óssea/efeitos da radiação , Efeito Espectador/genética , Proliferação de Células/efeitos da radiação , Dano ao DNA/genética , Epigênese Genética/genética , Instabilidade Genômica/genética , Instabilidade Genômica/efeitos da radiação , Células Germinativas/patologia , Masculino , Mutação/genética , Ratos , Ratos Long-Evans , Fatores Sexuais , Espermatócitos/patologia , Espermatócitos/efeitos da radiação , Testículo/patologia , Testículo/fisiopatologia , Testículo/efeitos da radiação , Timo/patologia , Timo/fisiopatologia , Timo/efeitos da radiaçãoRESUMO
It has been widely reported that prenatal exposure to ionizing radiation can interfere with embryonic and fetal development, depending on dose and gestational age in which exposure occurs. According to several studies on animal models, different well-defined stages during prenatal life can be distinguished in relation to teratogenic effects. During the preimplantation stage, elevated doses of radiation can result in abortion, while lower doses may produce genomic damage that is usually repaired. On the other hand, during the organogenesis stage in mice (embryonic day 6.5 [E6.5] to E13.5), irradiation is associated with increased incidence of malformation and intrauterine growth restriction (IUGR). Later exposure is linked to brain damage. Doses used in animal studies are generally higher than those used for diagnostic procedures in humans. Usually, radiation exposure to diagnostic range (<0.05 Gy = 5 rads) is not associated with an increased risk of congenital anomalies. In human studies, elevated doses produce adverse outcomes, depending on stage of development, as in animal studies. Blastogenesis (up to two weeks) is associated with failure to implant or no significant health effects. An increased risk of malformation and growth retardation can be observed for two to seven weeks exposure (organogenesis stage), while exposure at later stages (fetogenesis) is mainly associated with brain damage. In this review we focus on the relevance of estimating the cumulative dose of radiation to the fetus and the gestational age in which exposure occurs, to provide appropriate counseling to pregnant women.
Assuntos
Feto/efeitos da radiação , Efeitos Tardios da Exposição Pré-Natal , Anormalidades Induzidas por Radiação/etiologia , Anormalidades Induzidas por Radiação/genética , Aborto Espontâneo/etiologia , Animais , Sistema Nervoso Central/efeitos da radiação , Desenvolvimento Infantil/efeitos da radiação , Pré-Escolar , Relação Dose-Resposta à Radiação , Feminino , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Camundongos , Gravidez , Lesões por Radiação/embriologia , Lesões Experimentais por Radiação/embriologiaRESUMO
The purpose of the present research was the estimation of probable genetic consequences at the liquidators of the consequences of Chernobyl accident in 1986-1987. The research is made on two groups of the liquidators. The first group included the liquidators taking place on the account in the branch register and working now at the enterprises of a nuclear industry. The second group included 902 liquidators of consequences of Chernobyl accident in 1986 constantly living in the Ryazan area and which are taking place on permanent observation the account in regional hospital. For an estimation of probable genetic effects analyzed the data on frequency and outcomes pregnancy of the wives of the liquidators, on condition and on diseases of newborn, on switching intrauterine development defects (IDD). The analysis carried out depending on dozes of an irradiation: up to 5 cGy; 5-10 cGy and 10-25 cGy. Received materials testify, that at the liquidators, at a doze of an external irradiation 10-25 cGy, the determined effects--period long sterility, kept at a part them till 3 years come to light. The set of the received data, such as depending from the dose increase of frequency of spontaneous abortions and of inherent defects of development of newborn, the increase of frequency diseases of newborn and share newborn with low weight, allows to make a conclusion about an induction of genetic effects in sexual cells of the liquidators of consequences of Chernobyl accident at dozes of an external irradiation more than 10 cGy. Taking into account high biological efficiency of alpha-radiation (K = 20), and of beta-radiation (K = 2-4), the equivalent effective doze male gonads (testes) in 3-5 times is higher, than estimated only from external gamma-radiation.
Assuntos
Anormalidades Induzidas por Radiação , Acidente Nuclear de Chernobyl , Exposição Materna/efeitos adversos , Exposição Ocupacional/efeitos adversos , Exposição Paterna/efeitos adversos , Efeitos Tardios da Exposição Pré-Natal , Anormalidades Induzidas por Radiação/epidemiologia , Anormalidades Induzidas por Radiação/etiologia , Anormalidades Induzidas por Radiação/genética , Adulto , Feminino , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Gravidez , Efeitos Tardios da Exposição Pré-Natal/epidemiologia , Efeitos Tardios da Exposição Pré-Natal/etiologia , Efeitos Tardios da Exposição Pré-Natal/genética , Doses de Radiação , Federação Russa/epidemiologiaRESUMO
PURPOSE: To determine the impact of ultraviolet (UV) laser radiation on Bombyx mori embryos in terms of its effect on embryonic and larval haemolymph proteins and morphological traits. MATERIALS AND METHODS: The eggs of silkworm strain NB4D2 were exposed to third harmonic laser pulses at 355 nm from a Nd:YAG laser for different durations of 30, 60, 90, 120, 150, or 180 sec. Morphological changes induced by the UV laser were analysed at larval, pupal and adult stages. The eggs exposed to UV laser irradiation at different developmental stages were subjected to protein analysis by sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE). The haemolymph derived from irradiated and control larva was also analysed by SDS-PAGE. RESULTS: UV laser irradiation resulted in various structural polymorphisms. Asymmetrical fusion of segments was not confined to larva but persisted throughout pupal and adult stages. Development of extra caudal horn, unequal size and lack of antenna, retarded thoracic legs and variation in larval markings were observed. Comparatively, the effect of the UV laser on 8- and 16-h old embryo was greater than on the other stages. The changes in protein pattern were not distinct until the 5th day of embryogenesis as revealed by SDS-PAGE. A 178 kiloDalton (kDa) protein resolved into 198, 184 & 169 kDa polypeptides and 154 kDa new protein band along with other proteins of 110, 45, 41 & 38 kDa were noticed in irradiated eggs at the 6th day. Further, 33, 32, and 6.2 kDa new protein bands were observed in the haemolymph of 5th instar silkworm larvae derived from UV laser irradiated embryos. CONCLUSIONS: A comparative analysis of the present study revealed that UV laser not only induced continuous structural polymorphisms (somatic mutations), but also induced protein changes with the appearance of new protein bands in embryonic and haemolymph protein. The UV laser could be a potential tool for biochemical genetics and genome analysis in B. mori.
Assuntos
Anormalidades Induzidas por Radiação/embriologia , Anormalidades Induzidas por Radiação/genética , Bombyx/embriologia , Bombyx/efeitos da radiação , Lipoproteínas/metabolismo , Morfogênese/efeitos da radiação , Raios Ultravioleta/efeitos adversos , Animais , Relação Dose-Resposta à Radiação , Lipoproteínas/efeitos da radiação , Mutação , Doses de RadiaçãoRESUMO
The congenital malformation gastroschisis has a genetic disposition in the inbred mouse strain HLG/Zte. It is increased after preconceptional irradiation of males or females. Radiation exposures during the meiotic stages are most efficient. This malformation can also be induced by ionising radiation when the exposure takes place during the preimplantation period especially during the zygote stage. This latter effect can be transmitted to the next mouse generation. Other macroscopically visible or skeletal malformations are not significantly induced under these experimental conditions. These latter malformations are increased by radiation exposures during major organogenesis. The mechanisms for the development of the effects are different. Radiation exposure of the mouse zygote (1 to 3 hours p.c.) also leads to the induction of genomic instability in skin fibroblasts of the fetus. This phenomenon also occurs in a mouse strain (C57BL/6J) which is not susceptible to radiation-induced gastroschisis during the preimplantation period. The genomic instability is transmitted to the next mouse generation. During genomic instability chromatide breaks are dominating as in non-exposed cells. With respect to "spontaneous" malformations gastroschisis is dominating in HLG/Zte mice. Late radiation effects seem to have similar patterns as observed in non-exposed subjects, however, the rates are increased after irradiation.
Assuntos
Anormalidades Induzidas por Radiação/genética , Instabilidade Genômica/efeitos da radiação , Lesões Experimentais por Radiação/genética , Anormalidades Induzidas por Radiação/embriologia , Anormalidades Induzidas por Radiação/etiologia , Animais , Feminino , Gastrosquise/etiologia , Gastrosquise/genética , Predisposição Genética para Doença , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Lesões Pré-Concepcionais , Gravidez , Lesões Experimentais por Radiação/embriologia , Lesões Experimentais por Radiação/etiologia , Aberrações dos Cromossomos Sexuais , Zigoto/efeitos da radiaçãoRESUMO
Genetic studies in the offspring of atomic bomb survivors have been conducted since 1948 at the Atomic Bomb Casualty Commission and its successor, the Radiation Effects Research Foundation, in Hiroshima and Nagasaki. Past studies include analysis of birth defects (untoward pregnancy outcome; namely, malformation, stillbirth, and perinatal death), chromosome aberrations, alterations of plasma and erythrocyte proteins as well as epidemiologic study on mortality (any cause) and cancer incidence (the latter study is still ongoing). There is, thus far, no indication of genetic effects in the offspring of survivors. Recently, the development of molecular biological techniques and human genome sequence databases made it possible to analyze DNA from parents and their offspring (trio-analysis). In addition, a clinical program is underway to establish the frequency of adult-onset multi-factorial diseases (diabetes mellitus, high blood pressure, and cardiovascular disease etc) in the offspring. The complementary kinds of data that will emerge from this three-pronged approach (clinical, epidemiologic, and molecular aspects) promise to shed light on health effects in the offspring of radiation-exposed people.
Assuntos
Mutação , Armas Nucleares , Lesões por Radiação/genética , Anormalidades Induzidas por Radiação/genética , Anormalidades Induzidas por Radiação/história , Adulto , Animais , Criança , Aberrações Cromossômicas , Análise Citogenética , Feminino , História do Século XX , História do Século XXI , Humanos , Recém-Nascido , Japão , Masculino , Camundongos , Neoplasias Induzidas por Radiação/genética , Neoplasias Induzidas por Radiação/história , Armas Nucleares/história , Gravidez , Resultado da Gravidez , Lesões por Radiação/história , Fatores de Risco , Razão de MasculinidadeRESUMO
Parental exposure of mice to radiation and chemicals causes a variety of adverse effects (e.g., tumors, congenital malformations and embryonic deaths) in the progeny and the tumor-susceptibility phenotype is transmissible beyond the first post-radiation generation. The induced rates of tumors were 100-fold higher than those known for mouse specific locus mutations. There were clear strain differences in the types of naturally-occurring and induced tumors and most of the latter were malignant. Another important finding was that germ-line exposure elicited very weak tumorigenic responses, but caused persistent hypersensitivity in the offspring for the subsequent development of cancer by the postnatal environment. Activations of oncogenes, ras, mos, abl, etc. and mutations in tumor suppressor genes such as p53 were also detected in specific tumors in cancer-prone descendants. However, the majority of tumors observed in the progeny were those commonly observed in the strains that were used and oncogene activations were rarely observed in these tumors. It can be hypothesized that genetic instability modifies tumor occurrence in a transgenerational manner, but so far no links could be established between chromosomal and molecular changes and transmissible tumor risks. Our data are consistent with the hypothesis that cumulative changes in many normal but cancer-related genes affecting immunological, biochemical and physiological functions may slightly elevate the incidence of tumors or fasten the tumor development. This hypothesis is supported by our GeneChip analyses which showed suppression and/or over-expression of many such genes in the offspring of mice exposed to radiation. In humans, a higher risk of leukemia and birth defects has been reported in the children of fathers who had been exposed to radionuclides in the nuclear reprocessing plants and to diagnostic radiation. These findings have not been supported in the children of atomic bomb survivors in Hiroshima and Nagasaki, who were exposed to higher doses of atomic radiation. However, it will be important to follow the human subjects, especially for adult type cancers and chronic diseases throughout their lives to determine whether the mouse studies can predict human responses.