RESUMO
BACKGROUND: Root caries is preventable and can be arrested at any stage of disease development. The aim of this study was to investigate the potential mineral exchange and fluorapatite formation within artificial root carious lesions (ARCLs) using different toothpastes containing 5,000 ppm F, 1,450 ppm F or bioactive glass (BG) with 540 ppm F. MATERIALS AND METHODS: The crowns of each extracted sound tooth were removed. The remaining roots were divided into four parts (n = 12). Each sample was randomly allocated into one of four groups: Group 1 (Deionised water); Group 2 (BG with 540 ppm F); Group 3 (1,450 ppm F) and Group 4 (5,000 ppm F). ARCLs were developed using demineralisation solution (pH 4.8). The samples were then pH-cycled in 13 days using demineralisation solution (6 h) and remineralisation solution (pH 7) (16 h). Standard tooth brushing was carried out twice a day with the assigned toothpaste. X-ray Microtomography (XMT) was performed for each sample at baseline, following ARCL formation and after 13-day pH-cycling. Scanning Electron Microscope (SEM) and 19F Magic angle spinning nuclear magnetic resonance (19F-MAS-NMR) were also performed. RESULTS: XMT results showed that the highest mineral content increase (mean ± SD) was Group 4 (0.09 ± 0.05), whilst the mineral content decreased in Group 1 (-0.08 ± 0.06) after 13-day pH-cycling, however there was evidence of mineral loss within the subsurface for Groups 1, 3 and 4 (p < 0.05). SEM scans showed that mineral contents within the surface of dentine tubules were high in comparison to the subsurface in all toothpaste groups. There was evidence of dentine tubules being either partially or completely occluded in toothpaste groups. 19F-MAS-NMR showed peaks between - 103 and - 104ppm corresponding to fluorapatite formation in Groups 3 and 4. CONCLUSION: Within the limitation of this laboratory-based study, all toothpastes were potentially effective to increase the mineral density of artificial root caries on the surface, however there was evidence of mineral loss within the subsurface for Groups 1, 3 and 4.
Assuntos
Cárie Radicular , Cremes Dentais , Microtomografia por Raio-X , Projetos Piloto , Cremes Dentais/uso terapêutico , Humanos , Apatitas/uso terapêutico , Apatitas/análise , Concentração de Íons de Hidrogênio , Fluoretos/uso terapêutico , Remineralização Dentária/métodos , Cariostáticos/uso terapêutico , Técnicas In Vitro , Microscopia Eletrônica de VarreduraRESUMO
INTRODUCTION: This study was designed to determine the mineral composition of calculi in nephrocalcinosis with nephrolithiasis, diagnose the underlying disease, and monitor the course of renal function in patients with nephrocalcinosis-nephrolithiasis. METHODS: Renal calculi extruded in a series of 8 patients with nephrocalcinosis were analysed using Fourier transmission infrared spectrometry. In 4 patients, next-generation sequencing using a nephrocalcinosis-nephrolithiasis panel was performed to determine the nature of the underlying disease. In addition, longitudinal analysis of renal function was performed in all patients. RESULTS: Seven patients revealed carbonate apatite as the sole constituent of renal calculi. One patient showed a mixed composition of dicalcium phosphate dihydrate/carbonate apatite at first analysis yet in subsequent episodes also had calculi composed of pure carbonate apatite. Further molecular analysis displayed distal renal tubular acidosis in 2 of 4 patients who consented to sequencing. No known genetic defect could be found in the other two cases. In line with prior reports, decline of renal function was dependent on underlying disease. Distal renal tubular acidosis revealed a progressive course of renal failure, whereas other causes showed stable renal function in long term analysis. CONCLUSION: Nephrocalcinosis with nephrolithiasis is a rare condition with heterogeneous aetiology. Yet mineral composition of renal calculi predominantly consisted of pure carbonate apatite. This uniform finding is similar to subcutaneous calcifications of various origins and might propose a general principle of tissue calcification. Progressive decline of renal function was found in distal renal tubular acidosis, whereas other conditions remained stable over time.
Assuntos
Apatitas , Nefrocalcinose , Nefrolitíase , Humanos , Apatitas/análise , Nefrocalcinose/etiologia , Masculino , Nefrolitíase/etiologia , Feminino , Adulto , Pessoa de Meia-Idade , Acidose Tubular RenalRESUMO
The purpose of this study is to investigate the effect of orthodontic anchor screws (OASs) inserted into the femur of growth-phase or mature rats using histological observation and bone structure analysis. The experimental animals are growth-phase (6-week-old) or mature (25-week-old) male Wistar rats. OAS was placed into the point one-third of the femoral length from the proximal end of the femur, and the response of the surrounding bone was observed and measured. The results showed at the OAS bone interface, in growth-phase rats, bone mineral density (BMD) was reduced and the running angle of collagen fiber bundles varied significantly. In mature rats, more osteoid was observed and biological apatite (BAp) crystals showed a different orientation. It was suggested that after the insertion of OASs, bone volume and quality are decreased, but after a sufficient healing period, a new bone micro/nano structure, different from the original structure, are reconstructed.
Assuntos
Apatitas , Densidade Óssea , Ratos , Masculino , Animais , Densidade Óssea/fisiologia , Apatitas/análise , Ratos Wistar , Fêmur/cirurgia , Fêmur/química , Fêmur/fisiologia , Parafusos Ósseos , Colágeno/análiseRESUMO
Trace elements are recognized as being essential in dentin and bone apatite. The effects of zinc, strontium, magnesium, and iron were discussed in part I. In part II, we evaluated the functional role of copper, manganese, silicon, and lithium on dentin apatite, with critical effects on morphology, crystallinity, and solubility. An electronic search was performed on the role of these trace elements in dentin apatite from January 2000 to January 2022. The recent aspects of the relationship between four different trace elements and their critical role in the structure and mechanics of dentin were assessed. These findings show that elements play a vital role in the human body, especially in the crystalline structure of dentin apatite. Copper presents immense benefits in dental restorative biomaterials because of its importance in enhancing odontogenesis. The biological role of manganese in dentin apatite is still largely unknown, but it has gained attention for many of its broad physiological functions such as modulating osteoblast proliferation, differentiation, and metabolism in bones. The functional role of silicon in dentin apatite is similarly lacking, but findings reveal its importance in mineralization and collagen formation, making it useful for the field of restorative dentistry. Likewise, lithium was found to have important roles in dentin mineralization as well as in the formation of dentin bridges and tissues. Therefore, there is growing importance in studying the aforementioned elements in the context of dentin apatite.
Assuntos
Oligoelementos , Apatitas/análise , Cobre/análise , Cobre/farmacologia , Dentina/química , Humanos , Íons , Lítio/análise , Lítio/farmacologia , Manganês/análise , Manganês/farmacologia , Silício/farmacologia , Oligoelementos/análiseRESUMO
Dentin hydroxyapatite possesses a unique versatile structure which allows it to undergo ionic substitutions. Trace elements play pivotal roles within the oral cavity, especially in dentin apatite tissue. Therefore, it is critical to explore the role of these elements in dentin apatite structure. The roles of other inorganic elements in dentin apatite were discussed in part I (Mg, Sr, Zn, and Fe) and part II (Cu, Mn, Si, and Li) of these series. In the last part of the review series, the role of selenium, fluorine, silver, and boron in the regulation of dentin apatite structure and function was discussed. We evaluated how these elements affect the overall size, morphology, and crystallinity of dentin apatite crystals. Moreover, we investigated the importance of these elements in regulating the solubility of dentin apatite. An electronic search was performed on the role of these trace elements in dentin apatite from January 2010 to January 2022. The concentration of selenium in teeth has been explored only recently, particularly its incorporation into dentin apatite. Silver nanomaterials inhibit the growth of cariogenic microorganisms as well as arrest the degradation of collagen. Fluorine was found to have important roles in dentin remineralization and dentinal tubule occlusion, making it widely used for hydroxyapatite doping. Boron is critical for mineralized tissues like bone, dentin, and enamel, but its exact role in dentin apatite is unknown. Therefore, understanding the impact of these elements on dentin apatite is potentially transformative, as it may help to fill a significant knowledge gap in teeth mechanics.
Assuntos
Apatitas , Dentina , Oligoelementos , Apatitas/análise , Boro/análise , Dentina/química , Fluoretos/análise , Flúor/análise , Hidroxiapatitas/análise , Selênio/análise , Prata/análise , Oligoelementos/análiseRESUMO
Elevated particles and phosphorus washed from road-deposited sediment (RDS) are noteworthy causes of eutrophication in urban water bodies. Identifying how urban elements (e.g., dwellings, roads) spatially influence RDS and the associated phosphorus can help pinpoint the primary management areas for RDS pollution and therefore effectively mitigate this problem. This study investigated spatial influence of urban elements on RDS build-up load and phosphorus load in Hanyang district of Wuhan city in central China. Bayesian Networks (BNs), combined with geographical detector (Geodetector) and correlation analysis, were applied to quantify spatial association between kernel density of urban elements, RDS build-up load and phosphorus load in RDS. Results showed that (1) areas with higher density of factories related elements usually had elevated level of RDS build-up load, aluminum-bound phosphorus (Al-P), occluded phosphorus (Oc-P), organophosphorus (Or-P). Higher load of RDS associated iron-bound phosphorus (Fe-P) and apatite phosphorus (Ca-P) usually occurred where dwellings, catering, and entertainment related elements were concentrated. (2) Urban elements mainly showed positive correlation with RDS build-up load, Fe-P, Ca-P, De-P (detrital apatite phosphorus), while they chiefly showed negative correlation with Ex-P (exchangeable phosphorus), Al-P, Oc-P, and Or-P. Bus stations, dwellings, and factories related elements had relatively strong determinant power over spatial stratified heterogeneity of RDS and RDS-associated phosphorus. (3) Geodetector and correlation analysis could boost factors filtering and construction of network structures in the process of developing BNs models. The developed BNs resulted in sound prediction of <150 µm RDS build-up load and phosphorus load, given that the prediction accuracy of models ranged from 0.532 to 0.657. These findings demonstrate that urban elements are useful spatial predictors of RDS pollution, and coupling Geodetector and BNs is promising in RDS pollution prediction and supporting urban nonpoint source pollution management.
Assuntos
Fósforo , Poluentes Químicos da Água , Apatitas/análise , Teorema de Bayes , China , Monitoramento Ambiental , Sedimentos Geológicos/análise , Fósforo/análise , Poluentes Químicos da Água/químicaRESUMO
Many essential elements exist in nature with significant influence on dentin and bone apatite tissue. Hydroxyapatite (HAp) is the major inorganic crystalline structure of dentin that provides a site for various physiological functions such as surface layer ion exchange. Decades of apatite research have shown that enamel is a high-substituted crystalline apatite, but recent findings suggest that dentin apatite may play a more important role in regulating ion exchange as well as mineral crystallinity. This article is the first part of a review series on the functional role of inorganic trace elements including magnesium, strontium, zinc, and iron in dentin hydroxyapatite. The morphology, physiology, crystallinity, and solubility of these elements as they get substituted into the HAp lattice are extensively discussed. An electronic search was performed on the role of these elements in dentin apatite from January 2007 to September 2021. The relationship between different elements and their role in the mineral upkeep of dentin apatite was evaluated. Several studies recognized the role of these elements in dentinal apatite composition and its subsequent effects on morphology, crystallinity, and solubility. These elements are of great importance in physiological processes and an essential part of living organisms. Magnesium and strontium stimulate osteoblast activity, while zinc can improve overall bone quality with its antibacterial properties. Iron nanoparticles are also vital in promoting bone tissue growth as they donate or accept electrons in redox reactions. Thus, understanding how these elements impact dentin apatite structure is of great clinical significance.
Assuntos
Apatitas , Oligoelementos , Apatitas/análise , Oligoelementos/análise , Magnésio/análise , Difração de Raios X , Zinco/análise , Ferro/análise , Hidroxiapatitas/análise , Dentina/química , Estrôncio/análise , Durapatita/análise , Durapatita/químicaRESUMO
OBJECTIVES: Kidney stone formation is complex; urinary protein inhibitors play a major role in natural defense against stone formation. Using attenuated total-reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy of kidney stones, proteins are usually not quantified and often reported as 'organic matrix', for which there is little attention: treatment of urolithiasis is based on the nature of the major organic/inorganic stone compound. Literature no longer regards urinary proteins as innocent bystander, but highlights the role of proteins as urolithiasis modulators. We explored the potential significance of the protein content of kidney stones. METHODS: 800 stones were analyzed using ATR-FTIR spectroscopy; spectra were corrected for protein content. The ratio of the amide I peak (1655 cm-1) divided by the maximum peak was calculated. A subgroup of stones (n = 43) was weighed; protein concentration was assayed. Kidney stone composition was taken into account when calculating protein concentration. Electrophoresis was implemented to investigate the protein bands. Multiple regression analysis was carried out to study the influence of various demographic variables (age, gender, stone type) on protein concentration. RESULTS: Protein concentration showed a marked variation according to the stone composition. High relative protein content (>0.4% stone mass) was found in mixed calcium apatite/calcium oxalate dihydrate stones, mixed calcium oxalate dihydrate/calcium oxalate monohydrate/calcium apatite stones, and mixed calcium oxalate monohydrate/brushite stones, whereas lower protein percentages were found in cystine, urate, and calcium oxalate monohydrate stones. Protein concentration was dependent of the patient's age. CONCLUSION: ATR-FTIR is a practical way for assessing protein concentration in kidney stones. LIST OF ABBREVIATIONS: A: absorbance; as, asymmetric vibrations; ATR-FTIR, attenuated total-reflectance Fourier-transform infrared; ß, standardized regression coefficient; CAP, calcium apatite; COD, calcium oxalate dihydrate; COM, calcium oxalate monohydrate; CV, coefficient of variation; δ, bending vibrations; ELISA, enzyme-linked immunosorbent assay; IQR, interquartile range; IR, infrared; LOD, limit of detection; LOQ, limit of quantification; MIR, mid-infrared; N or n, amount; r, correlation; r2, coefficient of determination; s, symmetric vibrations; SD, standard deviation; SE, standard error; THP, Tamm-Horsfall protein; UA, uric acid; V, stretching vibrations; VIF: variance inflation factor; ZnSe, zinc selenide.
Assuntos
Oxalato de Cálcio , Cálculos Renais , Apatitas/análise , Cálcio , Oxalato de Cálcio/análise , Humanos , Cálculos Renais/química , Cálculos Renais/diagnóstico , Espectroscopia de Infravermelho com Transformada de Fourier , Ácido Úrico/análiseRESUMO
New types of biological apatites have been discovered in molar sheep and horse teeth and are divided in two types. In the first and more general type, the release of Mg ions is parallel to the changes in composition of apatite leading to a final stoichiometric ratio of Ca to P ions, going from dentin depth towards the boundary of enamel with air. Inside dentin, another apatite sub-types were discovered with alternating layers of Mg-rich and C-rich apatites. The approximate formal stoichiometric relationships for these peculiar types of bioapatites are suggested. We identified two kinds of ion-exchanges responsible for formation of peculiar apatites. Various combinations of main and minor elements lead to new versions of biological apatites.
Assuntos
Apatitas/análise , Magnésio/análise , Dente/química , Animais , Cálcio/análise , Dentina/química , Cavalos , Dente Molar/química , Fósforo/análise , OvinosRESUMO
Potassium releasing bioactive glasses (BAGs) may offer improved relief for dentine hypersensitivity compared to conventional sodium containing BAGs by releasing K+ ions for nerve desensitization and occluding dentinal tubules to prevent fluid flow within dentinal tubules. Potassium oxide was substituted for sodium oxide on a molar basis in a fluoride containing BAG used in toothpastes for treating dentine hypersensitivity. The BAG powders were then immersed in an artificial saliva at pH 7 and tris buffer and the pH rise and ion release behavior were characterized by ICP-OES and ISE. The potassium and sodium containing BAGs were characterized by XRD, DSC, FTIR and NMR. Both BAGs presented amorphous diffraction patterns and the glass transition temperature of the potassium glass was higher than that of the sodium glass. The 31P MAS-NMR spectra indicated a peak at 2.7 ppm corresponding to apatite and a small peak at -103 ppm indicated crystallization to fluorapatite. Both BAGs dissolved and formed apatite at similar rates, although the dissolution of the potassium glass was slightly slower and it released less fluoride as a result of partial nanocrystallization to fluorapatite upon quenching. The potassium release from the potassium ions could potentially result in nerve deactivation when used in toothpastes.
Assuntos
Cerâmica/análise , Flúor/análise , Potássio/análise , Cremes Dentais/análise , Apatitas/análise , Materiais Biocompatíveis/análise , Dessensibilizantes Dentinários/análise , Fluoretos/análise , Humanos , SolubilidadeRESUMO
Freezing bone samples to preserve their biomolecular properties for various analyses at a later time is a common practice. Storage temperature and freeze-thaw cycles are well-known factors affecting degradation of molecules in the bone, whereas less is known about the form in which the tissue is most stable. In general, as little intervention as possible is advised before storage. In the case of DNA analyses, homogenization of the bone shortly before DNA extraction is recommended. Because recent research on the DNA yield from frozen bone fragments and frozen bone powder indicates better DNA preservation in the latter, the aim of the study presented here was to investigate and compare the chemical composition of both types of samples (fragments versus powder) using ATR-FTIR spectroscopy. Pairs of bone fragments and bone powder originating from the same femur of 57 individuals from a Second World War mass grave, stored in a freezer at - 20 °C for 10 years, were analyzed. Prior to analysis, the stored fragments were ground into powder, whereas the stored powder was analyzed without any further preparation. Spectroscopic analysis was performed using ATR-FTIR spectroscopy. The spectra obtained were processed and analyzed to determine and compare the chemical composition of both types of samples. The results show that frozen powdered samples have significantly better-preserved organic matter and lower concentrations of B-type carbonates, but higher concentrations of A-type carbonates and stoichiometric apatite. In addition, there are more differences in the samples with a low DNA degradation index and less in the samples with a high DNA degradation index. Because the results are inconsistent with the current understanding of bone preservation, additional research into optimal preparation and long-term storage of bone samples is necessary.
Assuntos
Osso e Ossos/química , DNA/análise , Fêmur/química , Preservação de Tecido/métodos , Apatitas/análise , Restos Mortais/química , Carbonatos/análise , Colágeno/análise , Degradação Necrótica do DNA , Congelamento , Humanos , Masculino , Mudanças Depois da Morte , Refrigeração , Eslovênia/etnologia , Espectroscopia de Infravermelho com Transformada de Fourier , Fatores de Tempo , II Guerra MundialRESUMO
Kidney stones frequently develop as an overgrowth on Randall's plaque (RP) which is formed in the papillary interstitium. The organic composition of RP is distinct from stone matrix in that RP contains fibrillar collagen; RP in tissue has also been shown to have two proteins that are also found in stones, but otherwise the molecular constituents of RP are unstudied. We hypothesized that RP contains unique organic molecules that can be differentiated from the stone overgrowth by fluorescence. To test this, we used micro-CT-guided polishing to expose the interior of kidney stones for multimodal imaging with multiphoton, confocal and infrared microscopy. We detected a blue autofluorescence signature unique to RP, the specificity of which was also confirmed in papillary tissue from patients with stone disease. High-resolution mineral mapping of the stone also showed a transition from the apatite within RP to the calcium oxalate in the overgrowth, demonstrating the molecular and spatial transition from the tissue to the urine. This work provides a systematic and practical approach to uncover specific fluorescence signatures which correlate with mineral type, verifies previous observations regarding mineral overgrowth onto RP and identifies a novel autofluorescence signature of RP demonstrating RP's unique molecular composition.
Assuntos
Apatitas/análise , Oxalato de Cálcio/análise , Cálculos Renais/química , Medula Renal/patologia , Imagem Óptica/métodos , Biópsia , Feminino , Humanos , Cálculos Renais/cirurgia , Medula Renal/química , Medula Renal/diagnóstico por imagem , Masculino , Microscopia Confocal/métodos , Imagem Multimodal/métodos , Nefrolitotomia Percutânea , Espectroscopia de Infravermelho com Transformada de Fourier , Ureteroscopia , Microtomografia por Raio-X/métodosRESUMO
Human bladder stones, surgically removed from a 4 years old boy, were studied by X-ray diffraction (XRD) and by electron microprobe analyses (EPMA). XRD data show that the bladder stones are mainly composed of struvite with minor apatite. Tiny particles, <10 µm in size, composed of mercury (Hg) and selenium (Se) were found using scanning electron microscopy (SEM) and quantitative analysis by wave-length dispersive system (WDS). On the basis of their composition, the particles consist of tiemannite, a rare mineral with the ideal formula HgSe. The young patient was not exposed to relevant mercury contamination and has no teeth fillings of amalgam. Although this observation is not conclusive, we suggest that Hg was introduced as methylmercury by food. The discovered tiemannite can be classified as endogenous mineral, i.e., directly precipitated from the same fluids that formed the host bladder stones. This assumption is supported by the fact that tiemannite and struvite can crystallize at the same temperature and pH values. As proposed for the formation of tiemannite previously reported in the liver of cetaceans, we suggest that the tiemannite in the human body represents a probable product of demethylation of Hg. In this contribution, we suggest that Hg and Se were initially collected by urine in the human body and finally precipitated to form tiemannite under appropriate chemical-physical conditions together with the formation of the host bladder stone. This observation suggests that the precipitation and accumulation of metals, including Hg and Se, in the human body can be considered a physiological response to eliminate part of these trace elements, thus enabling detoxification.
Assuntos
Mercúrio/análise , Microscopia Eletrônica de Varredura/métodos , Minerais/análise , Selênio/análise , Cálculos da Bexiga Urinária/química , Apatitas/análise , Pré-Escolar , Humanos , Masculino , Minerais/química , Análise de Ondaletas , Difração de Raios X/métodosRESUMO
A new discrimination method for the bioapatite materials bone, antler and ivory was developed using X-ray diffractometry and comprises non-invasive measurements in order to take valuable objects into account. Our approach deals with the analysis of peak intensity ratios resulting from several measurements on each object. For instance, the intensity ratio of the apatite reflections 002 and 310 has been described in the literature as representing the degree of apatite crystal orientation and varies depending on the sample orientation. The decisive factor for the material identification is the value dispersion of intensity ratios resulting from the total of all measurements on one object. This pattern of data points, visualised via kernel density estimation (KDE), is characteristic for ivory, bone and antler, respectively, and enables the discrimination of these materials. The observation is justifiable since apatite crystal orientation adapts to the collagen fibre arrangement which shows major differences between different sorts of bioapatite materials. The patterns of data points were received via analysis of 88 objects made of bone (n = 30), antler (n = 27) and ivory (n = 31). In order to verify several identifications X-ray computer tomography was supplemented. The presented method usefully supplements already existing approaches concerning microscopic, elementary and biochemical analyses.
Assuntos
Chifres de Veado/química , Osso e Ossos/química , Dente/química , Difração de Raios X/métodos , Animais , Apatitas/análise , Apresentação de Dados , Análise Espacial , Tomografia Computadorizada por Raios XRESUMO
Teleosauridae and Metriorhynchidae were thalattosuchian crocodylomorph clades that secondarily adapted to marine life and coexisted during the Middle to Late Jurassic. While teleosaurid diversity collapsed at the end of the Jurassic, most likely as a result of a global cooling of the oceans and associated marine regressions, metriorhynchid diversity was largely unaffected, although the fossil record of Thalattosuchia is poor in the Cretaceous. In order to investigate the possible differences in thermophysiologies between these two thalattosuchian lineages, we analysed stable oxygen isotope compositions (expressed as δ18O values) of tooth apatite from metriorhynchid and teleosaurid specimens. We then compared them with the δ18O values of coexisting endo-homeothermic ichthyosaurs and plesiosaurs, as well as ecto-poikilothermic chondrichthyans and osteichthyans. The distribution of δ18O values suggests that both teleosaurids and metriorhynchids had body temperatures intermediate between those of typical ecto-poikilothermic vertebrates and warm-blooded ichthyosaurs and plesiosaurs, metriorhynchids being slightly warmer than teleosaurids. We propose that metriorhynchids were able to raise their body temperature above that of the ambient environment by metabolic heat production, as endotherms do, but could not maintain a constant body temperature compared with fully homeothermic ichthyosaurs and plesiosaurs. Teleosaurids, on the other hand, may have raised their body temperature by mouth-gape basking, as modern crocodylians do, and benefited from the thermal inertia of their large body mass to maintain their body temperature above the ambient one. Endothermy in metriorhynchids might have been a by-product of their ecological adaptations to active pelagic hunting, and it probably allowed them to survive the global cooling of the Late Jurassic, thus explaining the selective extinction affecting Thalattosuchia at the Jurassic-Cretaceous boundary. This article is part of the theme issue 'Vertebrate palaeophysiology'.
Assuntos
Apatitas/análise , Regulação da Temperatura Corporal , Isótopos de Oxigênio/análise , Répteis/fisiologia , Dente/química , Jacarés e Crocodilos/fisiologia , Animais , Apatitas/química , Organismos Aquáticos/fisiologia , Evolução Biológica , Fósseis , Oceanos e MaresRESUMO
Iontophoresis is a noninvasive technique, based on the application of a constant low-intensity electric current to facilitate the release of a variety of drugs, whether ionized or not, through biological membranes. The objective of this research was to evaluate the effect of iontophoresis using different electric current intensities on the uptake of fluoride in dental enamel with artificial caries lesions. In this in vitro operator-blind experiment, bovine enamel blocks (n = 10/group) with caries-like lesions and predetermined surface hardness were randomized into 6 groups: placebo gel without fluoride applied with a current of 0.8 mA (negative control), 2% NaF gel without application of any current, and 2% NaF gel applied with currents of 0.1, 0.2, 0.4 and 0.8 mA. Cathodic iontophoresis was applied for 4 min. The concentration of loosely bound fluoride (calcium fluoride) and firmly bound fluoride (fluorapatite) was determined. The results were analyzed by the nonparametric Kruskal-Wallis and Dunn tests. Iontophoresis at 0.8 mA, combined with the application of fluoridated gel (2% NaF), increased fluoride uptake in enamel with caries-like lesions, as either calcium fluoride or fluorapatite.
Assuntos
Cariostáticos/farmacologia , Cárie Dentária , Esmalte Dentário/efeitos dos fármacos , Fluoretos/farmacologia , Iontoforese/métodos , Animais , Apatitas/análise , Fluoreto de Cálcio/análise , Bovinos , Modelos Animais de Doenças , Eletricidade , Dureza/efeitos dos fármacos , Distribuição Aleatória , Propriedades de Superfície/efeitos dos fármacos , Resultado do TratamentoRESUMO
RESUMEN Los materiales a base de silicato de calcio han demostrado ser bioactivos debido a su capacidad para producir apatita carbonatada biológicamente compatible. El objetivo de este estudio fue analizar la bioactividad de Biodentine™ y MTA Repair HP® en contacto con discos de dentina humana, que se obturaron y dividieron aleatoriamente para formar cuatro grupos: grupo 1 Biodentine™, grupo 2 MTA Repair HP®, grupo control positivo MTA Angelus® y grupo control negativo IRM®, los cuales se incubaron en solución PBS durante 10 días, para posterior análisis por medio de MEB-EDS y Espectroscopía Raman. Los tres materiales a base de silicato de calcio analizados en este estudio demostraron ser bioactivos pues al entrar en contacto con una solución a base de fosfato desencadenaron la precipitación inicial de fosfato de calcio amorfo, que actúa como precursor durante la formación de apatita carbonatada.
ABSTRACT Calcium silicate-based materials have been shown to be bioactive due to their ability to produce biologically compatible carbonated apatite. The objective of this study was to analyze the bioactivity of Biodentine ™ and MTA Repair HP® in contact with human dentine discs, which were sealed and divided randomly to form four groups: group 1 Biodentine™, group 2 MTA Repair HP®, positive control group MTA Angelus® and negative control group IRM®, which were incubated in PBS solution for 10 days, for a subsequent analysis by means of MEB-EDS and Raman spectroscopy. The three calcium-based materials analyzed in this study proved to be bioactive because upon contact with a phosphate-based solution they were triggered at the onset of amorphous calcium phosphate, as the precursor during the formation of carbonated apatite.
Assuntos
Apatitas/análise , Análise Espectral Raman , Calcarea Silicata/análise , Materiais Dentários/análise , Sinergismo FarmacológicoRESUMO
Ash of Antarctic krill integument (AAKI) was prepared by sintering the integument at 550°C under air atmosphere for 4 hours, and its composition was analyzed by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR) and electron dispersive spectroscopy (EDS). XRD results showed that the major phase in AAKI was ascribed to apatite. Besides, it was noticed that the (300) peak of AAKI shifted to 33.07°, which was coincident with that of fluorapatite (FA). The FTIR results confirmed the presence of phosphate ions, and the absence of -OH. The EDS results confirmed the presence of Ca, P, O and F elements in the ash sample. The content of FA in the ash was determined to be 50.4%, and the proportion of fluorine in the form of FA to the total fluorine in the integument was 40.5%. Based on the XRD, FTIR and EDS results, it can be concluded that FA was the main form of fluoride in the integument of Antarctic krill.
Assuntos
Apatitas/análise , Euphausiacea/química , Fluoretos/análise , Sais/análise , Animais , Regiões Antárticas , Flúor/análise , Fosfatos/análise , Espectroscopia de Infravermelho com Transformada de Fourier , Difração de Raios XAssuntos
Cálculos Renais/diagnóstico por imagem , Adulto , Apatitas/análise , Feminino , Humanos , Cálculos Renais/química , Cálculos Renais/complicações , Cálculos Renais/microbiologia , Dor Lombar/etiologia , Proteus mirabilis/isolamento & purificação , Estruvita/análise , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVES: Multi-tissue stable isotope models to reconstruct past diets (Froehle, Kellner, & Schoeninger, 2012; Kellner & Schoeninger, 2007) have lacked data from a heavily C4 -dependent population. Using new data from southern African agriculturalists, published models are evaluated for accuracy in dietary reconstruction and applicability to isotopically diverse diets. Additionally, isotopic variation between tooth enamel and bone apatite, which are often treated as isotopically equivalent, is investigated. MATERIALS AND METHODS: δ13 Cbone apatite , δ13 Ctooth enamel , δ13 Ccollagen , and δ15 Ncollagen values for 51 adult southern African agriculturalists are presented. Bivariate (linear) and multivariate (cluster analysis, discriminant function analysis) models are recreated including these data, and the resulting dietary reconstructions evaluated against what we know of archaeological diets. RESULTS: Δ13 Ccollagen-enamel (5.67 ± 1.66) is significantly larger than Δ13 Ccollagen-bone apatite (4.77 ± 1.42) and are significantly different from each other (Mann Whitney U-Test, p = 0.0). δ13 Cbone apatite and δ13 Ctooth enamel were uncorrelated (R2 = 0.24). The agriculturalists consumed highly variable and heterogeneous diets, (mean δ13 Cbone apatite = -6.25 ± 2.49, δ13 Ctooth enamel = -2.88 ± 2.48, δ13 Ccollagen = -8.65 ± 2.16, δ15 Ncollagen = 10.05 ± 1.9). Multi- and bi-variate models under-estimate the probable contribution of C3 energy sources, and recreation of cluster analysis results in a significant reduction in the parsimony of the dietary clusters derived in Froehle et al., 2012. CONCLUSION: Bone apatite and tooth enamel are distinct biominerals, and their δ13 C values should not be treated as equivalent. Multiple tissue isotopes provide valuable insight into diet that cannot be achieved with single tissues, but current models are limited by the lack of isotopic diversity in the data on which they are based.