RESUMO
Ticks are blood-sucking arthropods of great importance in the medical and veterinary fields worldwide. They are considered second only to mosquitos as vectors of pathogenic microorganisms that can cause serious infectious disorders, such as Lyme borreliosis and tick-borne encephalitis. Hard (Ixodid) ticks feed on host animals for several days and inject saliva together with pathogens to hosts during blood feeding. Some animal species can acquire resistance to blood-feeding by ticks after a single or repeated tick infestation, resulting in decreased weights and numbers of engorged ticks or the death of ticks in subsequent infestations. Importantly, this acquired tick resistance (ATR) can reduce the risk of pathogen transmission from pathogen-infected ticks to hosts. This is the basis for the development of tick antigen-targeted vaccines to forestall tick infestation and tick-borne diseases. Accumulation of basophils is detected in the tick re-infested skin lesion of animals showing ATR, and the ablation of basophils abolishes ATR in mice and guinea pigs, illustrating the critical role for basophils in the expression of ATR. In this review article, we provide a comprehensive overview of recent advances in our understanding of the cellular and molecular mechanisms responsible for the development and manifestation of ATR, with a particular focus on the role of basophils.
Assuntos
Basófilos/imunologia , Memória Imunológica , Mordeduras e Picadas de Insetos/imunologia , Saliva/imunologia , Pele/imunologia , Doenças Transmitidas por Carrapatos/prevenção & controle , Carrapatos/imunologia , Animais , Basófilos/microbiologia , Basófilos/parasitologia , Basófilos/virologia , Histamina/imunologia , Liberação de Histamina , Interações Hospedeiro-Patógeno , Humanos , Imunoglobulina E/imunologia , Mordeduras e Picadas de Insetos/microbiologia , Mordeduras e Picadas de Insetos/parasitologia , Mordeduras e Picadas de Insetos/virologia , Saliva/microbiologia , Saliva/parasitologia , Saliva/virologia , Pele/microbiologia , Pele/parasitologia , Pele/virologia , Doenças Transmitidas por Carrapatos/etiologia , Doenças Transmitidas por Carrapatos/imunologia , Doenças Transmitidas por Carrapatos/transmissão , Carrapatos/microbiologia , Carrapatos/parasitologia , Carrapatos/virologia , Vacinação , Vacinas/uso terapêuticoRESUMO
Mast cells and basophils are central players in allergic reactions triggered by immunoglobulin E (IgE). They have intracellular granules containing allergic mediators (e.g., histamine, serotonin, inflammatory cytokines, proteases and ß-hexosaminidase), and stimulation by IgE-allergen complex leads to the release of such allergic mediators from the granules, that is, degranulation. Mast cells are residents of mucosal surfaces, including those of nasal and oral cavities, and play an important role in the innate defense system. Members of the mitis group streptococci such as Streptococcus oralis, are primary colonizers of the human oral cavity. They produce hydrogen peroxide (H2O2) as a by-product of sugar metabolism. In this study, we investigated the effects of streptococcal infection on RBL-2H3 mast cell/basophil cell line. Infection by oral streptococci did not induce degranulation of the cells. Stimulation of the RBL-2H3 cells with anti-dinitrophenol (DNP) IgE and DNP-conjugated human serum albumin triggers degranulation with the release of ß-hexosaminidase. We found that S. oralis and other mitis group streptococci inhibited the IgE-triggered degranulation of RBL-2H3 cells. Since mitis group streptococci produce H2O2, we examined the effect of S. oralis mutant strain deficient in producing H2O2, and found that they lost the ability to suppress the degranulation. Moreover, H2O2 alone inhibited the IgE-induced degranulation. Subsequent analysis suggested that the inhibition of degranulation was related to the cytotoxicity of streptococcal H2O2. Activated RBL-2H3 cells produce interleukin-4 (IL-4); however, IL-4 production was not induced by streptococcal H2O2. Furthermore, an in vivo study using the murine pollen-induced allergic rhinitis model suggested that the streptococcal H2O2 reduces nasal allergic reaction. These findings reveal that H2O2 produced by oral mitis group streptococci inhibits IgE-stimulated degranulation by inducing cell death. Consequently, streptococcal H2O2 can be considered to modulate the allergic reaction in mucosal surfaces.
Assuntos
Alérgenos/metabolismo , Hipersensibilidade/imunologia , Imunoglobulina E/imunologia , Infecções Estreptocócicas/tratamento farmacológico , Alérgenos/imunologia , Animais , Basófilos/imunologia , Basófilos/microbiologia , Basófilos/patologia , Degranulação Celular/imunologia , Sobrevivência Celular/imunologia , Dinitrofenóis/farmacologia , Humanos , Peróxido de Hidrogênio/metabolismo , Hipersensibilidade/tratamento farmacológico , Hipersensibilidade/patologia , Imunoglobulina E/metabolismo , Interleucina-4/genética , Interleucina-4/metabolismo , Mastócitos/imunologia , Mastócitos/microbiologia , Mastócitos/patologia , Camundongos , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Albumina Sérica Humana/imunologia , Albumina Sérica Humana/metabolismo , Infecções Estreptocócicas/imunologia , Streptococcus oralis/imunologia , Streptococcus oralis/patogenicidade , Açúcares/metabolismoRESUMO
Basophils are primarily associated with immunomodulatory functions in allergic diseases and parasitic infections. Recently, it has been demonstrated that both activated human and mouse basophils can form extracellular DNA traps (BETs) containing mitochondrial DNA and granule proteins. In this report, we provide evidence that, in spite of an apparent lack of phagocytic activity, basophils can kill bacteria through BET formation.
Assuntos
Bactérias/imunologia , Basófilos/imunologia , Basófilos/microbiologia , Armadilhas Extracelulares/imunologia , Armadilhas Extracelulares/microbiologia , Animais , Basófilos/metabolismo , Humanos , Hipersensibilidade/imunologia , Hipersensibilidade/microbiologia , Imunomodulação , Camundongos , Neutrófilos/imunologia , Neutrófilos/metabolismo , Neutrófilos/microbiologia , Fagocitose/imunologiaAssuntos
Basófilos/microbiologia , Ectima/microbiologia , Infecções Oportunistas/microbiologia , Infecções por Pseudomonas/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Sepse/microbiologia , Pele/irrigação sanguínea , Vênulas/microbiologia , Idoso , Antibacterianos/uso terapêutico , Basófilos/imunologia , Basófilos/patologia , Biópsia , Ectima/tratamento farmacológico , Ectima/imunologia , Ectima/patologia , Evolução Fatal , Humanos , Hospedeiro Imunocomprometido , Masculino , Insuficiência de Múltiplos Órgãos/microbiologia , Infecções Oportunistas/tratamento farmacológico , Infecções Oportunistas/imunologia , Infecções Oportunistas/patologia , Infecções por Pseudomonas/tratamento farmacológico , Infecções por Pseudomonas/imunologia , Infecções por Pseudomonas/patologia , Pseudomonas aeruginosa/imunologia , Sepse/tratamento farmacológico , Sepse/imunologia , Sepse/patologia , Resultado do Tratamento , Vênulas/imunologia , Vênulas/patologiaRESUMO
Mammalian defensins are cationic, antimicrobial peptides that play a central role in innate immunity. The peptides are composed of three structural subfamilies: α-, ß-, and θ-defensins. θ-defensins are macrocyclic octadecapeptides expressed only in Old World monkeys and orangutans and are produced by the pair-wise, head-to-tail splicing of nonapeptides derived from their respective precursors. The existence of three active θ-defensin genes predicts that six different RTDs (1-6) are produced in this species. In this study, we isolated and quantified RTDs 1-6 from the neutrophils of 10 rhesus monkeys. RTD-1 was the most abundant θ-defensin, constituting ~50% of the RTD content; total RTD content varied by as much as threefold between animals. All peptides tested were microbicidal at â¼1 µM concentrations. The contribution of θ-defensins to macaque neutrophil antimicrobial activity was assessed by analyzing the microbicidal properties of neutrophil granule extracts after neutralizing θ-defensin content with a specific antibody. θ-defensin neutralization markedly reduced microbicidal activities of the corresponding extracts. Macaque neutrophil granule extracts had significantly greater microbicidal activity than those of human neutrophils, which lack θ-defensins. Supplementation of human granule extracts with RTD-1 markedly increased the microbicidal activity of these preparations, further demonstrating a prominent microbicidal role for θ-defensins.
Assuntos
Grânulos Citoplasmáticos/imunologia , Grânulos Citoplasmáticos/microbiologia , Defensinas/fisiologia , Neutrófilos/imunologia , Neutrófilos/microbiologia , Animais , Basófilos/imunologia , Basófilos/metabolismo , Basófilos/microbiologia , Extratos Celulares/genética , Extratos Celulares/imunologia , Extratos Celulares/metabolismo , Grânulos Citoplasmáticos/metabolismo , Defensinas/biossíntese , Defensinas/genética , Feminino , Humanos , Macaca mulatta , Masculino , Neutrófilos/metabolismo , Isoformas de Proteínas/biossíntese , Isoformas de Proteínas/genética , Isoformas de Proteínas/fisiologia , Precursores de Proteínas/biossíntese , Precursores de Proteínas/genética , Precursores de Proteínas/fisiologiaRESUMO
Peptostreptococcus magnus protein L is a multidomain bacterial surface protein that correlates with virulence. It consists of up to five homologous Ig-binding domains (B1-B5) that interact with the variable domain of Ig kappa L chains. Intact protein L stimulates the synthesis and the release of IL-4 and IL-13 from human basophils in vitro. A protein L fragment covering the Ig-binding domains B1-B4 also induced IL-4 and IL-13 release from basophils. There was an excellent correlation (r(s) = 0.82; p < 0.001) between the maximal percent IL-4 release induced by protein L and that induced by anti-IgE and between intact protein L and the B1-B4 fragment (r(s) = 0.90; p < 0.01). Removal of IgE bound to basophils markedly reduced the IL-4 release induced by anti-IgE, protein L, and B1-B4. Preincubation of basophils with protein L or anti-IgE caused complete cross-desensitization to subsequent challenge with the heterologous stimulus. IgE purified from myeloma patients PS and PP (lambda chains) blocked anti-IgE-induced IL-4 release, but not the releasing activity of protein L. In contrast, IgE purified from myeloma patient ADZ (kappa chains) blocked both anti-IgE- and protein L-induced secretion. Cyclosporin A, but not cyclosporin H, inhibited protein L-induced release of IL-4 and IL-13 from basophils. Thus, protein L acts as a bacterial Ig superantigen to induce the synthesis and release of IL-4 and IL-13 from basophils by interacting with kappa L chains of the IgE isotype.
Assuntos
Proteínas de Bactérias/fisiologia , Basófilos/metabolismo , Proteínas de Ligação a DNA/fisiologia , Imunoglobulina E/fisiologia , Cadeias kappa de Imunoglobulina/fisiologia , Interleucina-13/metabolismo , Interleucina-4/metabolismo , Receptores de IgE/biossíntese , Superantígenos/fisiologia , Adolescente , Adulto , Anticorpos Anti-Idiotípicos/metabolismo , Anticorpos Anti-Idiotípicos/fisiologia , Basófilos/efeitos dos fármacos , Basófilos/imunologia , Basófilos/microbiologia , Sítios de Ligação de Anticorpos , Células Cultivadas , Reações Cruzadas , Ciclosporina/farmacologia , Regulação da Expressão Gênica/imunologia , Liberação de Histamina/efeitos dos fármacos , Liberação de Histamina/imunologia , Humanos , Imunoglobulina E/imunologia , Imunoglobulina E/metabolismo , Cadeias kappa de Imunoglobulina/metabolismo , Interleucina-13/genética , Interleucina-4/genética , Cinética , Pessoa de Meia-Idade , Proteínas do Mieloma/metabolismo , Peptostreptococcus/imunologia , Peptostreptococcus/patogenicidade , RNA Mensageiro/biossíntese , Sequências Repetitivas de Aminoácidos/imunologiaRESUMO
Helicobacter pylori is important in the aetiology of peptic ulceration. Despite inducing an inflammatory response in the mucosa, the organism persists, suggesting that it has efficient protective mechanisms. Some bacterial and viral products modulate histamine secretion from inflammatory cells. Therefore, this study examined the modulatory effects of H. pylori preparations on histamine release from rat peritoneal mast cells and human basophils. Eleven clinical isolates of H. pylori were prepared in different ways: as whole washed bacteria, washed sonicated bacteria, and formalin-killed bacteria, and as outermembrane and lipopolysaccharide (LPS) extracts. Histamine release from mast cells or basophils was not elicited by any of these bacterial preparations alone. However, when mixed with various secretory stimulants, the bacterial preparations caused inhibition of histamine release from rat mast cells (calcium ionophore A23187, compound 48/80, concanavalin A, anti-rat IgE) and human basophils (A23187, N-formyl Met-Leu-Phe). The degree of inhibition ranged from 48% to 97%. These results indicate that H. pylori exerts an inhibitory effect on cells of the immune system that contributes to its persistence within the gastric mucosa.
Assuntos
Basófilos/microbiologia , Helicobacter pylori/fisiologia , Liberação de Histamina , Mastócitos/microbiologia , Animais , Basófilos/metabolismo , Calcimicina/farmacologia , Células Cultivadas , Concanavalina A/farmacologia , Feminino , Liberação de Histamina/efeitos dos fármacos , Humanos , Imunoglobulina E/farmacologia , Mastócitos/metabolismo , N-Formilmetionina Leucil-Fenilalanina/farmacologia , Ratos , p-Metoxi-N-metilfenetilamina/farmacologiaRESUMO
A number of mechanisms participate in virus-induced asthma. Previously, we described enhanced basophil histamine release (HR) during an experimentally induced rhinovirus infection and after in vitro incubation of peripheral blood mononuclear cells (PBMC) with influenza virus. This study extends our previous observations and examines the effect of influenza A virus on basophil leukotriene C4 (LTC4) release as well as the effect of T-cell depletion on virus-enhanced basophil HR. PBMC were isolated from ragweed-allergic subjects and incubated with live influenza A virus or control medium (allantoic fluid). After incubation with influenza A, ragweed antigen (AgE) stimulated LTC4 and HR were enhanced (P less than 0.05). To further define the role of T cells in virus-enhanced basophil secretion, PBMC were isolated and divided into two aliquots. In one aliquot, T cells were removed by magnetic bead separation of mouse monoclonal anti-CD3-coated lymphocytes. T-cell-depleted and nontreated PBMC suspensions were incubated with influenza A or control medium, collected, and challenged with AgE to release histamine. Basophil HR was enhanced in the virus-treated group of PBMC that had not undergone T-cell depletion. In contrast, virus incubation did not enhance HR in the T-cell-depleted fraction. Finally, preliminary analysis of the supernate from virus-treated leukocytes indicates the presence of interferon-gamma. These findings suggest that T cells, and their cytokine products, play an integral role in the process by which viruses enhance basophil HR.
Assuntos
Basófilos/fisiologia , Liberação de Histamina , Vírus da Influenza A/fisiologia , Proteínas de Plantas , Rinite Alérgica Sazonal/sangue , Linfócitos T/imunologia , Adulto , Alérgenos/farmacologia , Anticorpos Monoclonais , Antígenos de Plantas , Basófilos/efeitos dos fármacos , Basófilos/microbiologia , Sobrevivência Celular/efeitos dos fármacos , Eosinófilos/citologia , Eosinófilos/imunologia , Feminino , Fator Estimulador de Colônias de Granulócitos e Macrófagos/imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos/fisiologia , Humanos , Técnicas In Vitro , Interferon gama/imunologia , Interferon gama/fisiologia , Interleucina-3/imunologia , Interleucina-3/fisiologia , Interleucina-5/imunologia , Interleucina-5/fisiologia , Depleção Linfocítica , Masculino , Pólen , Rinite Alérgica Sazonal/imunologia , SRS-A/sangueRESUMO
We have demonstrated that human peripheral blood basophils released histamine on direct incubation with paramyxoviruses in vitro. Most histamine release occurred during the first 15 to 30 minutes after challenge, depending on the dose of virus used; release initiated by virus was complete by 1 hour. At a virus/cell ratio of 1:1, Sendai virus caused 41 +/- 9% histamine release, whereas parainfluenza type 3 (PI-3) virus caused 25 +/- 5% release and respiratory syncytial (RS) virus caused 19 +/- 5% release. Sendai, but not PI-3 or RS, also caused a decrease in cell number and release of lactic dehydrogenase; however, this apparent cell lysis did not account for all the histamine released. Incubation of cells with virus desensitized them to subsequent triggering by viruses but did not affect response of cells to other stimuli. Histamine release was dependent on the virus/cell ratio, temperature, and metabolic energy, but it was not strictly dependent on the presence of calcium in the extracellular medium. Histamine release was not affected by preincubation of cells with colchicine, suggesting that microtubules were not involved in the release process. Basophils desensitized by anti-IgE in the absence of calcium or treated with lactic acid to dissociate IgE molecules from membrane receptors released amounts of histamine similar to that of control basophils; thus, release was not initiated through Fc epsilon receptors. It was found, however, that histamine release by these viruses was greatly reduced when concanavalin A was used for desensitization. These data demonstrate that the respiratory viruses studied can cause direct nonimmunologic release of histamine from human basophils. Our findings provide evidence for another mechanism by which respiratory viruses can initiate inflammation.
Assuntos
Basófilos/microbiologia , Liberação de Histamina , Paramyxoviridae/fisiologia , Adulto , Basófilos/metabolismo , Calcimicina/farmacologia , Permeabilidade da Membrana Celular , Humanos , Técnicas In Vitro , L-Lactato Desidrogenase/análise , Paramyxoviridae/efeitos da radiaçãoRESUMO
Hematopoietic cells of the Japanese quail were transformed by avian erythroblastosis virus in vivo and in vitro. In both circumstances, the infected hematopoietic tissues exhibited a dual oncogenic response of erythroid and mast cell-basophil elements. The erythroid transformants escaped the avian erythroblastosis virus block in differentiation and progressed to hemoglobinization. Resulting basophilic cells were morphologically, biochemically, and ultrastructurally identical to mast cell-basophils observed in other species. None of the virally transformed cells actively produced reverse transcriptase activity. Nonproducer cell lines synthesized viral RNA and both v-erbA and v-erbB proteins. These results indicate that the Japanese quail has a viral target cell different from that of the chicken. The implications of a single bipotential transformation target yielding both erythroid and mast cell-basophil colonies is discussed.
Assuntos
Alpharetrovirus/patogenicidade , Vírus da Leucose Aviária/patogenicidade , Basófilos/microbiologia , Transformação Celular Viral , Células-Tronco Hematopoéticas/microbiologia , Mastócitos/microbiologia , Animais , Basófilos/patologia , Coturnix , Células-Tronco Hematopoéticas/patologia , Mastócitos/patologia , Microscopia Eletrônica , Testes de Precipitina , Proteínas Virais/análise , Replicação ViralRESUMO
Viral respiratory infections provoke asthma in many patients. In the following study we examined the effect of an in vitro incubation of influenza A on leukocyte histamine release. After incubation with a live influenza A (H3N2) virus, calcium ionophore A23187 (0.5, 1.0, and 1.5 microgram/ml)-induced leukocyte histamine release (HR) was enhanced (p less than 0.05). This effect was also found with heat- or ether-inactivated virus. Similarly, influenza A-exposed leukocytes had augmented leukocyte HR during subsequent incubation with ragweed AgE. Incubation of the leukocyte suspension with interferon (800 IU/ml) for 24 hr was also associated with enhanced HR to ragweed AgE. In contrast, interferon did not alter the calcium ionophore A23187 HR. Therefore, although interferon may mediate the enhanced leukocyte HR when ragweed AgE is the inciting stimulus, it does not change HR to the calcium ionophore.