RESUMO
Analysis of acyl coenzyme A thioesters (acyl-CoAs) is crucial in the investigation of a wide range of biochemical reactions and paves the way to fully understand the concerned metabolic pathways and their superimposed networks. We developed two methods for suspect screening of acyl-CoAs in bacterial cultures using a high-resolution Orbitrap Fusion tribrid mass spectrometer. The methods rely on specific fragmentation patterns of the target compounds, which originate from the coenzyme A moiety. They make use of the formation of the adenosine 3',5'-diphosphate key fragment (m/z 428.0365) and the neutral loss of the adenosine 3'-phosphate-5'-diphosphate moiety (506.9952) as preselection criteria for the detection of acyl-CoAs. These characteristic ions are generated either by an optimised in-source fragmentation in a full scan Orbitrap measurement or by optimised HCD fragmentation. Additionally, five different filters are included in the design of method. Finally, data-dependent MS/MS experiments on specifically preselected precursor ions are performed. The utility of the methods is demonstrated by analysing cultures of the denitrifying betaproteobacterium "Aromatoleum" sp. strain HxN1 anaerobically grown with hexanoate. We detected 35 acyl-CoAs in total and identified 24 of them by comparison with reference standards, including all 9 acyl-CoA intermediates expected to occur in the degradation pathway of hexanoate. The identification of additional acyl-CoAs provides insight into further metabolic processes occurring in this bacterium. The sensitivity of the method described allows detecting acyl-CoAs present in biological samples in highly variable abundances. Graphical abstract.
Assuntos
Acil Coenzima A/metabolismo , Betaproteobacteria/metabolismo , Acil Coenzima A/análise , Betaproteobacteria/química , Betaproteobacteria/citologia , Técnicas de Cultura de Células/métodos , Cromatografia Líquida , Ésteres/análise , Ésteres/metabolismo , Espectrometria de Massas por Ionização por Electrospray/métodosRESUMO
The polytheonamides are among the most complex and biosynthetically distinctive natural products known to date. These potent peptide cytotoxins are derived from a ribosomal precursor processed by 49 mostly non-canonical posttranslational modifications. As the producer is a 'microbial dark matter' bacterium only distantly related to any cultivated organism, >70-step chemical syntheses have been developed to access these unique compounds. Here, we mined prokaryotic diversity to establish a synthetic platform based on the new host Microvirgula aerodenitrificans that produces hypermodified peptides within two days. Using this system, we generated the aeronamides, new polytheonamide-type compounds with near-picomolar cytotoxicity. Aeronamides, as well as the polygeonamides produced from deep-rock biosphere DNA, contain the highest numbers of D-amino acids in known biomolecules. With increasing bacterial genomes being sequenced, similar host mining strategies might become feasible to access further elusive natural products from uncultivated life.
Assuntos
Amidas/metabolismo , Produtos Biológicos/metabolismo , Peptídeos/genética , Peptídeos/metabolismo , Amidas/química , Betaproteobacteria/química , Betaproteobacteria/metabolismo , Produtos Biológicos/química , Peptídeos/química , Biologia SintéticaRESUMO
A Gram-negative, aerobic, motile by flagella, and light yellow bacterium, designated SS1-76T, was isolated from sediment of the Nakdong River in Sangju-si, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate SS1-76T belongs to the genus Uliginosibacterium of the family Rhodocyclaceae, exhibiting high sequence similarity with the type strains of Uliginosibacterium gangwonense 5YN10-9T (96.0%) and Uliginosibacterium paludis KBP-13T (94.9%). Strain SS1-76T contains ubiquinone-8 as a respiratory quinone and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), C16:0, and C14:0 as major fatty acids. The cellular polar lipids are composed of phosphatidylethanolamine, phosphatidylglycerol, and unidentified aminophospholipids. The DNA G+C content was 65.3 mol%. Phenotypic, chemotaxonomic, and phylogenetic evidence clearly indicated that strain SS1-76T represents a novel species of the genus Uliginosibacterium, for which the name Uliginosibacterium sangjuense sp. nov. is proposed. The type strain is SS1-76T (= KCTC 52159T = JCM 31375T).
Assuntos
Betaproteobacteria/classificação , Sedimentos Geológicos/microbiologia , Rios/microbiologia , Betaproteobacteria/química , Betaproteobacteria/genética , Betaproteobacteria/isolamento & purificação , Metabolômica/métodos , Tipagem Molecular , Fenótipo , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNARESUMO
Due to high-strength of organic matters, nutrients and pathogen, swine wastewater is a major source of pollution to rural environment and surface water. A sequencing-batch membrane bioreactor (SMBR) system with an automatic control strategy was developed for high-strength swine wastewater treatment. Short-cut nitrification and denitrification (SND) was achieved at nitrite accumulation rate of 83.6%, with removal rates of COD, NH4+-N and TN at 95%, 99% and 93%, respectively, at reduced HRT of 6.0 d and TN loading rate of 0.02kgN/(kgVSS d). With effective membrane separation, the reduction of total bacteria (TB) and putative pathogen were 2.77 logs and 1%, respectively. The shift of microbial community was well responded to controlling parameters. During the SND process, ammonia oxidizing bacteria (AOB) (Nitrosomonas, Nitrosospira) and nitrite oxidizing bacteria (NOB) (Nitrospira) were enriched by 52 times and reduced by 2 times, respectively. The denitrifiers (Thauera) were well enriched and the diversity was enhanced.
Assuntos
Amônia/metabolismo , Betaproteobacteria/metabolismo , Reatores Biológicos/microbiologia , Nitritos/química , Águas Residuárias/microbiologia , Amônia/química , Animais , Bactérias , Betaproteobacteria/química , Desnitrificação , Nitrificação , Suínos , Águas Residuárias/químicaRESUMO
Some bacteria are capable of forming flocs, in which bacterial cells become self-flocculated by secreted extracellular polysaccharides and other biopolymers. The floc-forming bacteria play a central role in activated sludge, which has been widely utilized for the treatment of municipal sewage and industrial wastewater. Here, we use a floc-forming bacterium, Aquincolatertiaricarbonis RN12, as a model to explore the biosynthesis of extracellular polysaccharides and the regulation of floc formation. A large gene cluster for exopolysaccharide biosynthesis and a gene encoding the alternative sigma factor RpoN1, one of the four paralogues, have been identified in floc formation-deficient mutants generated by transposon mutagenesis, and the gene functions have been further confirmed by genetic complementation analyses. Interestingly, the biosynthesis of exopolysaccharides remained in the rpoN1-disrupted flocculation-defective mutants, but most of the exopolysaccharides were secreted and released rather than bound to the cells. Furthermore, the expression of exopolysaccharide biosynthesis genes seemed not to be regulated by RpoN1. Taken together, our results indicate that RpoN1 may play a role in regulating the expression of a certain gene(s) involved in the self-flocculation of bacterial cells but not in the biosynthesis and secretion of exopolysaccharides required for floc formation.IMPORTANCE Floc formation confers bacterial resistance to predation of protozoa and plays a central role in the widely used activated sludge process. In this study, we not only identified a large gene cluster for biosynthesis of extracellular polysaccharides but also identified four rpoN paralogues, one of which (rpoN1) is required for floc formation in A. tertiaricarbonis RN12. In addition, this RpoN sigma factor regulates the transcription of genes involved in biofilm formation and swarming motility, as previously shown in other bacteria. However, this RpoN paralogue is not required for the biosynthesis of exopolysaccharides, which are released and dissolved into culture broth by the rpoN1 mutant rather than remaining tightly bound to cells, as observed during the flocculation of the wild-type strain. These results indicate that floc formation is a regulated complex process, and other yet-to-be identified RpoN1-dependent factors are involved in self-flocculation of bacterial cells via exopolysaccharides and/or other biopolymers.
Assuntos
Proteínas de Bactérias/metabolismo , Betaproteobacteria/metabolismo , Polissacarídeos Bacterianos/biossíntese , Fator sigma/metabolismo , Proteínas de Bactérias/genética , Betaproteobacteria/química , Betaproteobacteria/genética , Floculação , Regulação Bacteriana da Expressão Gênica , Fator sigma/genéticaRESUMO
Although several works have been performed from an engineering point of view, a limited number of studies have focused on microbial communities involved in the humification of aged landfill leachates. In this work, cultivation techniques, next-generation sequencing, and phospholipid fatty acid analysis were adopted to decrypt the diversity and the ecophysiological properties of the dominant microbiota in aged landfill leachate. Based on Illumina sequencing, Betaproteobacteria, Bacteroidetes, Actinobacteria, and Alphaproteobacteria dominated the aged landfill leachate. The main taxa identified at genus level were Pusillimonas-like bacteria and Leucobacter (41.46% of total reads), with all of them being also isolated through cultivation. The presence of Pusillimonas-like bacteria was also verified by the detection of cyclo17:0 and iso-19:0 fatty acids in aged landfill leachate microbiota. Despite that almost all bacterial isolates exhibited extracellular lipolytic ability, no particular specificity was observed in the type of substrate utilized. The prevalence of effective degraders, such as Pusillimonas-like bacteria, makes the aged landfill leachate an ideal source for isolation of novel microorganisms with potential in situ bioremediation uses.
Assuntos
Águas Residuárias/microbiologia , Microbiologia da Água , Poluentes Químicos da Água/análise , Actinobacteria/química , Actinobacteria/genética , Actinobacteria/isolamento & purificação , Alphaproteobacteria/química , Alphaproteobacteria/genética , Alphaproteobacteria/isolamento & purificação , Bacteroidetes/química , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Betaproteobacteria/química , Betaproteobacteria/genética , Betaproteobacteria/isolamento & purificação , Biodegradação Ambiental , Análise da Demanda Biológica de Oxigênio , Microbiota , Fosfolipídeos/química , Filogenia , Instalações de Eliminação de Resíduos , Águas Residuárias/análise , Poluentes Químicos da Água/químicaRESUMO
Laribacter hongkongensis is a potential emerging pathogen associated with community-acquired gastroenteritis and traveler's diarrhea. We report the isolation of L. hongkongensis from the stool of a patient who had no history of travel outside the United States. The organism was identified by phenotypic tests, mass spectrometry, and gene sequencing.
Assuntos
Infecções Bacterianas/diagnóstico , Infecções Bacterianas/microbiologia , Betaproteobacteria/classificação , Betaproteobacteria/isolamento & purificação , Fezes/microbiologia , Gastroenterite/diagnóstico , Gastroenterite/microbiologia , Adolescente , Técnicas de Tipagem Bacteriana , Betaproteobacteria/química , Betaproteobacteria/fisiologia , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Feminino , Humanos , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Viagem , Estados UnidosRESUMO
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic approaches to produce antibiotics have been unable to replace this platform. Uncultured bacteria make up approximately 99% of all species in external environments, and are an untapped source of new antibiotics. We developed several methods to grow uncultured organisms by cultivation in situ or by using specific growth factors. Here we report a new antibiotic that we term teixobactin, discovered in a screen of uncultured bacteria. Teixobactin inhibits cell wall synthesis by binding to a highly conserved motif of lipid II (precursor of peptidoglycan) and lipid III (precursor of cell wall teichoic acid). We did not obtain any mutants of Staphylococcus aureus or Mycobacterium tuberculosis resistant to teixobactin. The properties of this compound suggest a path towards developing antibiotics that are likely to avoid development of resistance.
Assuntos
Antibacterianos/farmacologia , Depsipeptídeos/farmacologia , Resistência Microbiana a Medicamentos , Viabilidade Microbiana/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacos , Animais , Antibacterianos/biossíntese , Antibacterianos/química , Antibacterianos/isolamento & purificação , Betaproteobacteria/química , Betaproteobacteria/genética , Produtos Biológicos/química , Produtos Biológicos/isolamento & purificação , Produtos Biológicos/farmacologia , Parede Celular/química , Parede Celular/efeitos dos fármacos , Parede Celular/metabolismo , Depsipeptídeos/biossíntese , Depsipeptídeos/química , Depsipeptídeos/isolamento & purificação , Modelos Animais de Doenças , Resistência Microbiana a Medicamentos/genética , Feminino , Camundongos , Testes de Sensibilidade Microbiana , Dados de Sequência Molecular , Família Multigênica/genética , Mycobacterium tuberculosis/citologia , Mycobacterium tuberculosis/genética , Peptidoglicano/biossíntese , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/química , Staphylococcus aureus/citologia , Staphylococcus aureus/genética , Ácidos Teicoicos/biossíntese , Fatores de TempoRESUMO
Although uncultured microorganisms have important roles in ecosystems, their ecophysiology in situ remains elusive owing to the difficulty of obtaining live cells from their natural habitats. In this study, we employed a novel magnetic nanoparticle-mediated isolation (MMI) method to recover metabolically active cells of a group of previously uncultured phenol degraders, Burkholderiales spp., from coking plant wastewater biosludge; five other culturable phenol degraders-Rhodococcus sp., Chryseobacterium sp. and three different Pseudomonas spp.-were also isolated from the same biosludge using traditional methods. The kinetics of phenol degradation by MMI-recovered cells (MRCs) was similar to that of the original sludge. Stable isotope probing (SIP) and pyrosequencing of the 16S rRNA from the 'heavy' DNA ((13)C-DNA) fractions indicated that Burkholderiales spp. were the key phenol degraders in situ in the biosludge, consistent with the results of MRCs. Single-cell Raman micro-spectroscopy was applied to probe individual bacteria in the MRCs obtained from the SIP experiment and showed that 79% of them were fully (13)C-labelled. Biolog assays on the MRCs revealed the impact of various carbon and nitrogen substrates on the efficiency of phenol degradation in the wastewater treatment plant biosludge. Specifically, hydroxylamine, a metabolite of ammonia oxidisation, but not nitrite, nitrate or ammonia, inhibited phenol degradation in the biosludge. Our results provided a novel insight into the occasional abrupt failure events that occur in the wastewater treatment plant. This study demonstrated that MMI is a powerful tool to recover live and functional cells in situ from a complex microbial community to enable further characterisation of their physiology.
Assuntos
Betaproteobacteria/isolamento & purificação , Magnetismo/métodos , Nanopartículas de Magnetita/química , Águas Residuárias/microbiologia , Betaproteobacteria/química , Betaproteobacteria/genética , Betaproteobacteria/metabolismo , Biodegradação Ambiental , DNA Bacteriano/genética , Fenol/metabolismo , RNA Ribossômico 16S/genética , Esgotos/química , Esgotos/microbiologia , Águas Residuárias/químicaRESUMO
Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobacterium sp. strain J3 generated the I262V, F275W, Q282N, and Q282Y Oxy derivatives, which showed oxygenation capabilities different from those of the wild-type enzyme. To understand the structural features resulting in the different oxidation reactions, we determined the crystal structures of the derivatives, both free and complexed with substrates. The I262V, F275W, and Q282Y derivatives catalyze the lateral dioxygenation of carbazole with higher yields than the wild type. A previous study determined the crystal structure of Oxy complexed with carbazole and revealed that the carbonyl oxygen of Gly178 hydrogen bonds with the imino nitrogen of carbazole. In these derivatives, the carbazole was rotated approximately 15, 25, and 25°, respectively, compared to the wild type, creating space for a water molecule, which hydrogen bonds with the carbonyl oxygen of Gly178 and the imino nitrogen of carbazole. In the crystal structure of the F275W derivative complexed with fluorene, C-9 of fluorene, which corresponds to the imino nitrogen of carbazole, was oriented close to the mutated residue Trp275, which is on the opposite side of the binding pocket from the carbonyl oxygen of Gly178. Our structural analyses demonstrate that the fine-tuning of hydrophobic residues on the surface of the substrate-binding pocket in ROs causes a slight shift in the substrate-binding position that, in turn, favors specific oxygenation reactions toward various substrates.
Assuntos
Proteínas de Bactérias/química , Betaproteobacteria/enzimologia , Dioxigenases/química , Ferro/metabolismo , Oxigênio/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Betaproteobacteria/química , Betaproteobacteria/genética , Biocatálise , Carbazóis/metabolismo , Cristalografia por Raios X , Dioxigenases/genética , Dioxigenases/metabolismo , Modelos Moleculares , Mutagênese Sítio-Dirigida , OxirreduçãoRESUMO
Aerobic anoxygenic photosynthesis (AAP) is found in an increasing number of proteobacterial strains thriving in ecosystems ranging from extremely oligotrophic to eutrophic. Here, we have investigated whether the fuel oxygenate-degrading betaproteobacterium Aquincola tertiaricarbonis L108 can use AAP to compensate kinetic limitations at low heterotrophic substrate fluxes. In a fermenter experiment with complete biomass retention and also during chemostat cultivation, strain L108 was challenged with extremely low substrate feeding rates of tert-butyl alcohol (TBA), an intermediate of methyl tert-butyl ether (MTBE). Interestingly, formation of photosynthetic pigments, identified as bacteriochlorophyll a and spirilloxanthin, was only induced in growing cells at TBA feeding rates less than or equal to maintenance requirements observed under energy excess conditions. Growth continued at rates between 0.001 and 0.002 h(-1) even when the TBA feed was decreased to values close to 30â% of this maintenance rate. Partial sequencing of genomic DNA of strain L108 revealed a bacteriochlorophyll synthesis gene cluster (bchFNBHL) and photosynthesis regulator genes (ppsR and ppaA) typically found in AAP and other photosynthetic proteobacteria. The usage of light as auxiliary energy source enabling evolution of efficient degradation pathways for kinetically limited heterotrophic substrates and for lowering the threshold substrate concentration Smin at which growth becomes zero is discussed.
Assuntos
Betaproteobacteria/crescimento & desenvolvimento , Betaproteobacteria/metabolismo , Fotossíntese , terc-Butil Álcool/metabolismo , Anaerobiose , Bacterioclorofila A/análise , Betaproteobacteria/química , Betaproteobacteria/fisiologia , DNA Bacteriano/química , DNA Bacteriano/genética , Metabolismo Energético , Dados de Sequência Molecular , Análise de Sequência de DNA , Xantofilas/análiseRESUMO
Rubrivivax benzoatilyticus JA2 produces indole derivatives (indoles) from aniline, anthranilate or L-tryptophan. Glucose repressed indole production in R. benzoatilyticus JA2, while malate had no effect. Growth of R. benzoatilyticus JA2 on glucose resulted in decrease in culture pH (6.4) compared with malate (8.4). Growth of R. benzoatilyticus JA2 on sugar carbon sources decreased culture pH (6.4-6.6) and indole production. Further, culture pH of 6.4 repressed the indole production, and pH 8.4 promoted the production irrespective of carbon sources used for growth. Moreover, correlation between indole production and culture pH was observed, where acidic pH inhibited indole production, while alkaline pH promoted the production, suggesting the role of pH in indole production. Tryptophan-catabolizing enzyme activities are significantly high in malate-grown cultures (pH 8.4) compared with that of the glucose (pH 6.4)-grown cultures and corroborated well with indole production, indicating their role in indole production. These results confirm that indole production in R. benzoatilyticus JA2 is pH dependent rather than carbon catabolite repression.
Assuntos
Betaproteobacteria/metabolismo , Carbono/metabolismo , Indóis/metabolismo , Betaproteobacteria/química , Repressão Catabólica , Meios de Cultura/química , Meios de Cultura/metabolismo , Concentração de Íons de Hidrogênio , Triptofano/metabolismoRESUMO
The electron donor for periplasmic chlorate reductase of Ideonella dechloratans has been suggested to be a soluble cytochrome c. We describe here the purification of the 9-kDa periplasmic cytochrome c, denoted cytochrome c-Id1, and demonstrate its ability to serve as an electron donor for purified chlorate reductase. The reaction rate was found to be linearly dependent on the cytochrome c concentration in the range of 0.6-4 µM. A route for electron transport involving a soluble cytochrome c is similar to that found for other periplasmic oxidoreductases of the dimethyl sulfoxide reductase family, but different from that suggested for the (per)chlorate reductase of Dechloromonas species.
Assuntos
Betaproteobacteria/enzimologia , Citocromos c/química , Citocromos c/metabolismo , Oxirredutases/química , Oxirredutases/metabolismo , Betaproteobacteria/química , Citocromos c/isolamento & purificação , Transporte de Elétrons , Eletroforese em Gel de Poliacrilamida , Cinética , EspectrofotometriaRESUMO
Ammonia oxidation by microorganisms is a critical process in the nitrogen cycle. Recent research results show that ammonia-oxidizing archaea (AOA) are both abundant and diverse in a range of ecosystems. In this study, we examined the abundance and diversity of AOA and ammonia-oxidizing beta-proteobacteria (AOB) in estuarine sediments in Hong Kong for two seasons using the ammonia monooxygenase A subunit gene (amoA) as molecular biomarker. Relationships between diversity and abundance of AOA and AOB and physicochemical parameters were also explored. AOB were more diverse but less abundant than AOA. A few phylogenetically distinct amoA gene clusters were evident for both AOA and AOB from the mangrove sediment. Pearson moment correlation analysis and canonical correspondence analysis (CCA) were used to explore physicochemical parameters potentially important to AOA and AOB. Metal concentrations were proposed to contribute potentially to the distributions of AOA while total phosphorus (TP) was correlated to the distributions of AOB. Quantitative PCR estimates indicated that AOA were more abundant than AOB in all samples, but the ratio of AOA/AOB (from 1.8 to 6.3) was smaller than most other studies by one to two orders. The abundance of AOA or AOB was correlated with pH and temperature while the AOA/AOB ratio was with the concentrations of ammonium. Several physicochemical factors, rather than any single one, affect the distribution patterns suggesting that a combination of factors is involved in shaping the dynamics of AOA and AOB in the mangrove ecosystem.
Assuntos
Amônia/metabolismo , Archaea/isolamento & purificação , Betaproteobacteria/isolamento & purificação , Sedimentos Geológicos/microbiologia , Archaea/química , Archaea/classificação , Archaea/genética , Betaproteobacteria/química , Betaproteobacteria/classificação , Betaproteobacteria/genética , Biodiversidade , Biomarcadores/química , Biota , Clonagem Molecular , Meio Ambiente , Microbiologia Ambiental , Poluição Ambiental , Genes Arqueais , Genes Bacterianos , Sedimentos Geológicos/análise , Sedimentos Geológicos/química , Hong Kong , Concentração de Íons de Hidrogênio , Metais/química , Família Multigênica , Oxirredução , Oxirredutases/genética , Fósforo/química , Filogenia , Compostos de Amônio Quaternário/química , Rhizophoraceae , Estações do Ano , Especificidade da Espécie , TemperaturaRESUMO
Bioavailability of electron acceptors is probably the most limiting factor in the restoration of anoxic, contaminated environments. The oxidation of contaminants such as aromatic hydrocarbons, particularly in aquifers, often depends on the reduction of ferric iron or sulphate. We have previously detected a highly active fringe zone beneath a toluene plume at a tar-oil-contaminated aquifer in Germany, where a specialized community of contaminant degraders codominated by Desulfobulbaceae and Geobacteraceae had established. Although on-site geochemistry links degradation to sulphidogenic processes, dominating catabolic (benzylsuccinate synthase α-subunit, bssA) genes detected in situ appeared to be more related to those of Geobacter spp. Therefore, a stable isotope probing (SIP) incubation of sediment samples with (13)C(7)-toluene and comparative electron acceptor amendment was performed. We introduce pyrosequencing of templates from SIP microcosms as a powerful new strategy in SIP gradient interpretation (Pyro-SIP). Our results reveal the central role of Desulfobulbaceae in sulphidogenic toluene degradation in situ, and affiliate the detected bssA genes to this lineage. This and the absence of (13)C-labelled DNA of Geobacter spp. in SIP gradients preclude their relevance as toluene degraders in situ. In contrast, Betaproteobacteria related to Georgfuchsia spp. became labelled under iron-reducing conditions. Furthermore, secondary toluene degraders belonging to the Peptococcaceae detected in both treatments suggest the possibility of functional redundancy among anaerobic toluene degraders on site.
Assuntos
Técnicas de Tipagem Bacteriana/métodos , Água Subterrânea/microbiologia , Tolueno/metabolismo , Poluentes Químicos da Água/metabolismo , Sequência de Bases , Betaproteobacteria/química , Betaproteobacteria/classificação , Betaproteobacteria/metabolismo , Biodegradação Ambiental , Carbono-Carbono Liases , Deltaproteobacteria/genética , Deltaproteobacteria/metabolismo , Geobacter/química , Geobacter/classificação , Geobacter/metabolismo , Alemanha , Água Subterrânea/química , Hidrocarbonetos Aromáticos/metabolismo , Isótopos/química , Dados de Sequência Molecular , Oxidantes/química , Sulfatos/metabolismo , Alcatrões/metabolismoRESUMO
The purpose of this study is to evaluate whether sample preservation can affect the yield of nucleic acid extracts from environmental samples. Storage of microbial samples was studied using three sediment types of varying carbon contents (10-57% carbon of dry weight). Four different storage solutions were tested at three temperatures. Freezing of samples at -20 °C or -80 °C, either without preservative or in phenol-chloroform solution, retained nucleic acid quantities very efficiently. Storage of samples in phenol-chloroform solution at +4 °C also gave good yields except for sediment with extremely high-carbon content. Ethanol and RNAlater preservation decreased nucleic acid yields drastically at all temperatures. To study how sample preservation may affect the result of microbial community analysis, one type of sediment was selected for length heterogeneity-PCR analysis and PCR cloning of the 16S rRNA genes. Ethanol and RNAlater preservation caused a slight bias towards certain microbial types in the community analyses shown by underrepresentation of Bacteroidetes, Betaproteobacteria and Gammaproteobacteria-affiliated peak sizes and overrepresentation of Actinobacteria, Chloroflexi and Alphaproteobacteria-affiliated peak sizes. Based on the results of this study, preservation in phenol-chloroform solution can be recommended as an alternative storage method when freezing is not possible such as during extended field sampling; however, ethanol and RNAlater may cause serious problems when used as preservatives for environmental samples containing humic acids.
Assuntos
Congelamento , Preservação Biológica , RNA Bacteriano/metabolismo , RNA Ribossômico 16S/análise , Actinobacteria/química , Alphaproteobacteria/química , Betaproteobacteria/química , Gammaproteobacteria/química , Substâncias HúmicasRESUMO
In this study, we describe the antimycobacterial activity of two pigments, violacein, a purple violet pigment from Janthinobacterium sp. Ant5-2 (J-PVP), and flexirubin, a yellow-orange pigment from Flavobacterium sp. Ant342 (F-YOP). These pigments were isolated from bacterial strains found in the land-locked freshwater lakes of Schirmacher Oasis, East Antarctica. The minimum inhibitory concentrations (MICs) of these pigments for avirulent and virulent mycobacteria were determined by the microplate Alamar Blue Assay (MABA) and Nitrate Reductase Assay (NRA). Results indicated that the MICs of J-PVP and F-YOP were 8.6 and 3.6 µg/ml for avirulent Mycobacterium smegmatis mc²155; 5 and 2.6 µg/ml for avirulent Mycobacterium tuberculosis mc²6230; and 34.4 and 10.8 µg/ml for virulent M. tuberculosis H37Rv, respectively. J-PVP exhibited a ~15 times lower MIC for Mycobacterium sp. than previously reported for violacein pigment from Chromobacterium violaceum, while the antimycobacterial effect of F-YOP remains undocumented. Our results indicate these pigments isolated from Antarctic bacteria might be valuable lead compounds for new antimycobacterial drugs used for chemotherapy of tuberculosis.
Assuntos
Antibacterianos/farmacologia , Betaproteobacteria/química , Flavobacterium/química , Indóis/farmacologia , Mycobacterium/efeitos dos fármacos , Pigmentos Biológicos/farmacologia , Polienos/farmacologia , Regiões Antárticas , Antibacterianos/isolamento & purificação , Antituberculosos/isolamento & purificação , Antituberculosos/farmacologia , Descoberta de Drogas , Farmacorresistência Bacteriana , Indóis/isolamento & purificação , Testes de Sensibilidade Microbiana , Mycobacterium smegmatis/efeitos dos fármacos , Mycobacterium tuberculosis/efeitos dos fármacos , Pigmentos Biológicos/isolamento & purificação , Polienos/isolamento & purificaçãoRESUMO
Anaerobic chlorate respiration requires electron transport from the bacterial inner membrane to the soluble periplasmic chlorate reductase. We have recently demonstrated that soluble c cytochromes function as electron carriers for chlorate reduction in Ideonella dechloratans (Smedja Bäcklund et al. 2009). In the present work, we describe a gene encoding soluble c-type cytochrome [cyt; GenBank ID: EU768872] located close to the gene cluster for chlorate reduction in I. dechloratans. The predicted amino acid sequence does not match any of the peptide masses or partial sequences obtained earlier from periplasmic c cytochromes. The gene, without the predicted signal sequence, was expressed heterologously in E. coli and the recombinant protein was purified, refolded and reconstituted with heme. The reconstituted protein shows spectral properties characteristic for c cytochromes, with an absorption maximum at 553 nm for the alpha band in the reduced state. Pyridine hemochrome analysis demonstrates the formation of covalently bound heme.
Assuntos
Proteínas de Bactérias/genética , Betaproteobacteria/genética , Cloratos/metabolismo , Citocromos c/genética , Família Multigênica , Sequência de Aminoácidos , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Sequência de Bases , Betaproteobacteria/química , Betaproteobacteria/metabolismo , Citocromos c/química , Citocromos c/metabolismo , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
16S rRNA gene sequenced-based phylogeny indicates that Rhizobacter dauci ATCC 43778(T) branches within the radiation of Methylibium type strains. A comparative chemotaxonomic study including fatty acid methyl esters, polar lipids and polyamines reveals significant differences that, in combination with the topology of phylogenetic trees, support a dissection of the genus Methylibium. The proposals of this study include the transfer of Methylibium fulvum to the genus Rhizobacter as Rhizobacter fulvus comb. nov. (type strain Gsoil 322(T) =KCTC 12591(T) =DSM 19916(T)) and the reclassification of Methylibium aquaticum as Piscinibacter aquaticus gen. nov., comb. nov. (the type strain of Piscinibacter aquaticus is IMCC1728(T) =KCCM 42364(T) =NBRC 102349(T) =DSM 19915(T)) and of Methylibium subsaxonicum as Rivibacter subsaxonicus gen. nov., comb. nov. (the type strain of Rivibacter subsaxonicus is BF49(T) =DSM 19570(T) =CIP 109700(T)). As a consequence of these reclassifications, emended descriptions of the genera Methylibium and Rhizobacter are provided.
Assuntos
Betaproteobacteria/classificação , Pseudomonadaceae/classificação , Betaproteobacteria/química , Betaproteobacteria/genética , Betaproteobacteria/isolamento & purificação , DNA Bacteriano/genética , DNA Ribossômico/genética , Ácidos Graxos/química , Filogenia , Pseudomonadaceae/química , Pseudomonadaceae/genética , Pseudomonadaceae/isolamento & purificação , RNA Ribossômico 16S/genéticaRESUMO
The aim of this study was to clarify the pathway of electron transfer between the inner membrane components and the periplasmic chlorate reductase. Several soluble c-type cytochromes were found in the periplasm. The optical difference spectrum of dithionite-reduced periplasmic extract shows that at least one of these components is capable of acting as an electron donor to the enzyme chlorate reductase. The cytochromes were partially separated, and the fractions were analyzed by UV/visible spectroscopy to determine the ability of donating electrons to chlorate reductase. Our results show that one of the c cytochromes (6 kDa) is able to donate electrons, both to chlorate reductase and to the membrane-bound cytochrome c oxidase, whereas the roles of the remaining c cytochromes still remain to be elucidated. Peptide extracts of the c cytochromes were obtained by tryptic in-gel digestion for matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis. Peptide sequences obtained indicate that the 6-kDa cytochrome c protein is similar to c cytochromes from the chlorate-reducing bacterium Dechloromonas aromatica.