RESUMO
Root colonization by plant-growth-promoting bacteria could not be useful without the beneficial properties of the bacterium itself. Thus, it is necessary to evaluate the bacterial capacity to form biofilms and establish a successful interaction with the plant roots. We assessed the ability of growth-promoting bacterial strains to form biofilm and display chemotactic behaviour in response to organic acids and (or) root exudates of the model plant Brachypodium distachyon. This assessment was based on the evaluation of single strains of bacteria and a multispecies consortium. The strains coexisted together and formed biofilm under biotic (living root) and abiotic (glass) surfaces. Citric acid stimulated biofilm formation in all individual strains, indicating a strong chemotactic behaviour towards organic acids. Recognizing that the transition from single strains of bacteria to a "multicellular" system would not happen without the presence of adhesion, the alginate and exopolysaccharide (EPS) contents were evaluated. The EPS amounts were comparable in single strains and consortium forms. Alginate production increased 160% in the consortium subjected to drought stress (10% PEG). These findings demonstrated that (i) bacteria-bacteria interaction is the hub of various factors that would not only affect their relation but also could indirectly affect the balanced plant-microbe relation and (ii) root exudates could be very selective in recruiting a highly qualified multispecies consortium.
Assuntos
Biofilmes/crescimento & desenvolvimento , Brachypodium/química , Brachypodium/microbiologia , Quimiotaxia/fisiologia , Endófitos/fisiologia , Ácidos/farmacologia , Bactérias/efeitos dos fármacos , Bactérias/crescimento & desenvolvimento , Biofilmes/efeitos dos fármacos , Quimiotaxia/efeitos dos fármacos , Endófitos/efeitos dos fármacos , Interações entre Hospedeiro e Microrganismos , Consórcios Microbianos/efeitos dos fármacos , Exsudatos de Plantas/farmacologia , Raízes de Plantas/química , Raízes de Plantas/microbiologiaRESUMO
Gas chromatography-mass spectrometry (GC-MS) analysis revealed that castasterone and its biosynthetic precursors are found in Brachypodium distachyon. In vitro conversion experiments with crude enzyme solutions prepared from B. distachyon demonstrated the presence of the following biosynthetic sequences: campesterol â campesta-4-en-3-one â campesta-3-one â campestanol â 6-deoxocathasterone â 6-deoxoteasterone â teasterone â 3-dehydroteasterone â typhasterol â castasterone. campesterol â 22-hydroxycampesterol â 22-hydroxy-campesta-4-en-3-one â 22-hydroxy-campesta-3-one â 6-deoxo-3-dehydroteasterone â 3-dehydroteasterone. 6-deoxoteasterone â 6-deoxo-3-dehydroteasterone â 6-deoxotyphasterol â 6-deoxocastasterone â castasterone. This shows that there are campestanol-dependent and campestanol-independent pathway in B. distachyon that synthesize 24-methylated brassinosteroids (BRs). Biochemical analysis of BRs biosynthetic enzymes confirmed that BdDET2, BdCYP90B1, BdCYP90A1, BdCYP90D2, and BdCYP85A1 are orthologous to BR 5α-reductase, BR C-22 hydroxylase, BR C-3 oxidase, BR C-23 hydroxylase, and BR C-6 oxidase, respectively. Brassinolide was not identified in B. distachyon. Additionally, B. distachyon crude enzyme solutions could not catalyze the conversion of castasterone to brassinolide, and the gene encoding an ortholog of CYP85A2 (a brassinolide synthase) was not found in B. distachyon. These results strongly suggest that the end product for brassinosteroid biosynthesis which controls the growth and development of B. distachyon is not brassinolide but rather castasterone.
Assuntos
Brachypodium/metabolismo , Colestanóis/metabolismo , Vias Biossintéticas , Brachypodium/química , Brachypodium/genética , Brassinosteroides/biossíntese , Brassinosteroides/química , Colestanóis/química , Cromatografia Gasosa-Espectrometria de Massas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismoRESUMO
Plant cell walls mostly comprise polysaccharides and proteins. The composition of monocots' primary cell walls differs from that of dicots walls with respect to the type of hemicelluloses, the reduction of pectin abundance and the presence of aromatic molecules. Cell wall proteins (CWPs) differ among plant species, and their distribution within functional classes varies according to cell types, organs, developmental stages and/or environmental conditions. In this review, we go deeper into the findings of cell wall proteomics in monocot species and make a comparative analysis of the CWPs identified, considering their predicted functions, the organs analyzed, the plant developmental stage and their possible use as targets for biofuel production. Arabidopsis thaliana CWPs were considered as a reference to allow comparisons among different monocots, i.e., Brachypodium distachyon, Saccharum spp. and Oryza sativa. Altogether, 1159 CWPs have been acknowledged, and specificities and similarities are discussed. In particular, a search for A. thaliana homologs of CWPs identified so far in monocots allows the definition of monocot CWPs characteristics. Finally, the analysis of monocot CWPs appears to be a powerful tool for identifying candidate proteins of interest for tailoring cell walls to increase biomass yield of transformation for second-generation biofuels production.
Assuntos
Brachypodium/metabolismo , Parede Celular/metabolismo , Oryza/metabolismo , Proteínas de Plantas/metabolismo , Saccharum/metabolismo , Brachypodium/química , Metabolismo dos Carboidratos , Parede Celular/química , Metabolismo dos Lipídeos , Oryza/química , Oxirredutases/análise , Oxirredutases/isolamento & purificação , Oxirredutases/metabolismo , Peptídeo Hidrolases/análise , Peptídeo Hidrolases/isolamento & purificação , Peptídeo Hidrolases/metabolismo , Proteínas de Plantas/análise , Proteínas de Plantas/isolamento & purificação , Proteômica , Saccharum/química , Transdução de SinaisRESUMO
In plants, strict regulation of stomatal pores is critical for modulation of CO2 fixation and transpiration. Under certain abiotic and biotic stressors, pore closure is initiated through anionic flux, with calcium (Ca2+) playing a central role. The aluminum-activated malate transporter 12 (ALMT12) is a malate-activated, voltage-dependent member of the aluminum-activated malate transporter family that has been implicated in anionic flux from guard cells controlling the stomatal aperture. Herein, we report the characterization of the regulatory mechanisms mediating channel activities of an ALMT from the grass Brachypodium distachyon (BdALMT12) that has the highest sequence identity to Arabidopsis thaliana ALMT12. Electrophysiological studies in a heterologous cell system confirmed that this channel is malate- and voltage-dependent. However, this was shown to be true only in the presence of Ca2+ Although a general kinase inhibitor increased the current density of BdALMT12, a calmodulin (CaM) inhibitor reduced the Ca2+-dependent channel activation. We investigated the physiological relevance of the CaM-based regulation in planta, where stomatal closure, induced by exogenous Ca2+ ionophore and malate, was shown to be inhibited by exogenous application of a CaM inhibitor. Subsequent analyses revealed that the double substitutions R335A/R338A and R335A/K342A, within a predicted BdALMT12 CaM-binding domain (CBD), also decreased the channels' ability to activate. Using isothermal titration calorimetry and CBD-mimetic peptides, as well as CaM-agarose affinity pulldown of full-length recombinant BdALMT12, we confirmed the physical interaction between the CBD and CaM. Together, these findings support a co-regulatory mechanism of BdALMT12 activation by malate, and Ca2+/CaM, emphasizing that a complex regulatory network modulates BdALMT12 activity.
Assuntos
Brachypodium , Cálcio , Calmodulina , Transportadores de Ânions Orgânicos , Proteínas de Plantas , Estômatos de Plantas , Substituição de Aminoácidos , Brachypodium/química , Brachypodium/genética , Brachypodium/metabolismo , Cálcio/química , Cálcio/metabolismo , Calmodulina/química , Calmodulina/genética , Calmodulina/metabolismo , Ativação do Canal Iônico/fisiologia , Malatos/química , Malatos/metabolismo , Mutação de Sentido Incorreto , Transportadores de Ânions Orgânicos/química , Transportadores de Ânions Orgânicos/genética , Transportadores de Ânions Orgânicos/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Estômatos de Plantas/química , Estômatos de Plantas/genética , Estômatos de Plantas/metabolismoRESUMO
Mixed-linkage glucan (MLG) is a polysaccharide that is highly abundant in grass endosperm cell walls and present at lower amounts in other tissues. Cellulose synthase-like F (CSLF) and cellulose synthase-like H genes synthesize MLG, but it is unknown if other genes participate in the production and restructuring of MLG. Using Brachypodium distachyon transcriptional profiling data, we identified a B distachyon trihelix family transcription factor (BdTHX1) that is highly coexpressed with the B distachyon CSLF6 gene (BdCSLF6), which suggests that BdTHX1 is involved in the regulation of MLG biosynthesis. To determine the genes regulated by this transcription factor, we conducted chromatin immunoprecipitation sequencing (ChIP-seq) experiments using immature B distachyon seeds and an anti-BdTHX1 polyclonal antibody. The ChIP-seq experiment identified the second intron of BdCSLF6 as one of the most enriched sequences. The binding of BdTHX1 to the BdCSLF6 intron sequence was confirmed using electrophoretic mobility shift assays (EMSA). ChIP-seq also showed that a gene encoding a grass-specific glycoside hydrolase family 16 endotransglucosylase/hydrolase (BdXTH8) is bound by BdTHX1, and the binding was confirmed by EMSA. Radiochemical transglucanase assays showed that BdXTH8 exhibits predominantly MLG:xyloglucan endotransglucosylase activity, a hetero-transglycosylation reaction, and can thus produce MLG-xyloglucan covalent bonds; it also has a lower xyloglucan:xyloglucan endotransglucosylase activity. B distachyon shoots regenerated from transformed calli overexpressing BdTHX1 showed an abnormal arrangement of vascular tissue and seedling-lethal phenotypes. These results indicate that the transcription factor BdTHX1 likely plays an important role in MLG biosynthesis and restructuring by regulating the expression of BdCSLF6 and BdXTH8.
Assuntos
Brachypodium/genética , Glucanos/metabolismo , Glucosiltransferases/metabolismo , Glicosiltransferases/metabolismo , Fatores de Transcrição/metabolismo , Xilanos/metabolismo , Brachypodium/química , Brachypodium/enzimologia , Parede Celular/metabolismo , Glucosiltransferases/genética , Glicosiltransferases/genética , Íntrons/genética , Fenótipo , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plântula/química , Plântula/enzimologia , Plântula/genética , Especificidade da Espécie , Fatores de Transcrição/genéticaRESUMO
In contrast to the well-studied crystalline cellulose of microbial and animal origins, cellulose in plant cell walls is disordered due to its interactions with matrix polysaccharides. Plant cell wall (PCW) is an undisputed source of sustainable global energy; therefore, it is important to determine the molecular structure of PCW cellulose. The most reactive component of cellulose is the exocyclic hydroxymethyl group: when it adopts the tg conformation, it stabilizes intrachain and interchain hydrogen bonding, while gt and gg conformations destabilize the hydrogen-bonding network. So far, information about the hydroxymethyl conformation in cellulose has been exclusively obtained from 13C chemical shifts of monosaccharides and oligosaccharides, which do not reflect the environment of cellulose in plant cell walls. Here, we use solid-state Nuclear Magnetic Resonance (ssNMR) spectroscopy to measure the hydroxymethyl torsion angle of cellulose in two model plants, by detecting distance-dependent polarization transfer between H4 and H6 protons in 2D 13C-13C correlation spectra. We show that the interior crystalline portion of cellulose microfibrils in Brachypodium and Arabidopsis cell walls exhibits H4-H6 polarization transfer curves that are indicative of a tg conformation, whereas surface cellulose chains exhibit slower H4-H6 polarization transfer that is best fit to the gt conformation. Joint constraints by the H4-H6 polarization transfer curves and 13C chemical shifts indicate that it is unlikely for interior cellulose to have a significant population of the gt and gg conformation mixed with the tg conformation, while surface cellulose may adopt a small percentage of the gg conformation. These results provide new constraints to the structure and matrix interactions of cellulose in plant cell walls, and represent the first direct determination of a torsion angle in an important noncrystalline carbohydrate polymer.
Assuntos
Parede Celular/química , Celulose/análogos & derivados , Arabidopsis/química , Brachypodium/química , Configuração de Carboidratos , Espectroscopia de Ressonância Magnética/métodosRESUMO
Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) has become an important tool for the analysis of biomolecules, such as DNA, peptides, and oligosaccharides. This technique has been developed as a rapid, sensitive, and accurate means for analyzing cell wall polysaccharide structures. Here, we describe a method using mass spectrometry to provide xyloglucan composition and structure information of Brachypodium plants which will be useful for functional characterization of xyloglucan biosynthesis pathway in Brachypodium distachyon.
Assuntos
Brachypodium/química , Parede Celular/química , Glucanos/análise , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Xilanos/análise , Brachypodium/metabolismo , Parede Celular/metabolismo , Celulase/metabolismo , Glucanos/metabolismo , Oligossacarídeos/análise , Oligossacarídeos/metabolismo , Xilanos/metabolismoRESUMO
This chapter describes a method allowing the purification of the cell wall for studying both polysaccharides and proteins. The plant primary cell wall is mainly composed of polysaccharides (90-95 % in mass) and of proteins (5-10 %). At the end of growth, specialized cells may synthesize a lignified secondary wall composed of polysaccharides (about 65 %) and lignin (about 35 %). Due to its composition, the cell wall is the cellular compartment having the highest density and this property is used for its purification. It plays critical roles during plant development and in response to environmental constraints. It is largely used in the food and textile industries as well as for the production of bioenergy. All these characteristics and uses explain why its study as a true cell compartment is of high interest. The proposed method of purification can be used for large amount of material but can also be downscaled to 500 mg of fresh material. Tools for checking the quality of the cell wall preparation, such as protein analysis and microscopy observation, are also provided.
Assuntos
Arabidopsis/química , Brachypodium/química , Fracionamento Celular/métodos , Parede Celular/química , Proteínas de Plantas/isolamento & purificação , Vacúolos/química , Arabidopsis/crescimento & desenvolvimento , Brachypodium/crescimento & desenvolvimento , Fracionamento Celular/instrumentação , Parede Celular/ultraestrutura , Meios de Cultura/química , Eletroforese em Gel de Poliacrilamida/métodos , Hipocótilo/química , Hipocótilo/ultraestrutura , Microscopia , Proteínas de Plantas/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Sementes/química , Sementes/crescimento & desenvolvimentoRESUMO
BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99%), 142-3 (by 13.8%) and 406-1 (by 8.13%) compared with the wild type. Moreover, fermentable glucose was significantly higher in 135-2 (by 23.91%) and 142-3 (by 36.72%) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cell wall and could contribute to increases in bioethanol production using bioenergy crops. © 2016 Society of Chemical Industry.
Assuntos
Brachypodium/genética , Brachypodium/efeitos da radiação , Lignina/análise , Biocombustíveis , Brachypodium/química , Brachypodium/metabolismo , Parede Celular/química , Parede Celular/metabolismo , Parede Celular/efeitos da radiação , Raios gama , Lignina/metabolismo , Mutação , Caules de Planta/efeitos da radiaçãoRESUMO
Until recently, the 3D architecture of plant cell walls was poorly understood due to the lack of high-resolution techniques for characterizing the molecular structure, dynamics, and intermolecular interactions of the wall polysaccharides in these insoluble biomolecular mixtures. We introduced multidimensional solid-state NMR (SSNMR) spectroscopy, coupled with (13)C labelling of whole plants, to determine the spatial arrangements of macromolecules in near-native plant cell walls. Here we review key evidence from 2D and 3D correlation NMR spectra that show relatively few cellulose-hemicellulose cross peaks but many cellulose-pectin cross peaks, indicating that cellulose microfibrils are not extensively coated by hemicellulose and all three major polysaccharides exist in a single network rather than two separate networks as previously proposed. The number of glucan chains in the primary-wall cellulose microfibrils has been under active debate recently. We show detailed analysis of quantitative (13)C SSNMR spectra of cellulose in various wild-type (WT) and mutant Arabidopsis and Brachypodium primary cell walls, which consistently indicate that primary-wall cellulose microfibrils contain at least 24 glucan chains.
Assuntos
Parede Celular/química , Celulose/química , Arabidopsis/química , Brachypodium/química , Espectroscopia de Ressonância Magnética , Pectinas/química , Proteínas de Plantas/química , Polissacarídeos/químicaRESUMO
Lignin is a complex polyphenolic heteropolymer that is abundant in the secondary cell walls of plants and functions in growth and defence. It is also a major barrier to the deconstruction of plant biomass for bioenergy production, but the spatiotemporal details of how lignin is deposited in actively lignifying tissues and the precise relationships between wall lignification in different cell types and developmental events, such as flowering, are incompletely understood. Here, the lignin-detecting fluorogenic dye, Basic Fuchsin, was adapted to enable comparative fluorescence-based imaging of lignin in the basal internodes of three Brachypodium distachyon ecotypes that display divergent flowering times. It was found that the extent and intensity of Basic Fuchsin fluorescence increase over time in the Bd21-3 ecotype, that Basic Fuchsin staining is more widespread and intense in 4-week-old Bd21-3 and Adi-10 basal internodes than in Bd1-1 internodes, and that Basic Fuchsin staining reveals subcellular patterns of lignin in vascular and interfascicular fibre cell walls. Basic Fuchsin fluorescence did not correlate with lignin quantification by acetyl bromide analysis, indicating that whole-plant and subcellular lignin analyses provide distinct information about the extent and patterns of lignification in B. distachyon. Finally, it was found that flowering time correlated with a transient increase in total lignin, but did not correlate strongly with the patterning of stem lignification, suggesting that additional developmental pathways might regulate secondary wall formation in grasses. This study provides a new comparative tool for imaging lignin in plants and helps inform our views of how lignification proceeds in grasses.
Assuntos
Brachypodium/química , Corantes Fluorescentes/química , Lignina/química , Frações Subcelulares/químicaRESUMO
The polysaccharide structure and dynamics in the primary cell wall of the model grass Brachypodium distachyon are investigated for the first time using solid-state nuclear magnetic resonance (NMR). While both grass and non-grass cell walls contain cellulose as the main structural scaffold, the former contains xylan with arabinose and glucuronic acid substitutions as the main hemicellulose, with a small amount of xyloglucan (XyG) and pectins, while the latter contains XyG as the main hemicellulose and significant amounts of pectins. We labeled the Brachypodium cell wall with (13)C to allow two-dimensional (2D) (13)C correlation NMR experiments under magic-angle spinning. Well-resolved 2D spectra are obtained in which the (13)C signals of cellulose, glucuronoarabinoxylan (GAX), and other matrix polysaccharides can be assigned. The assigned (13)C chemical shifts indicate that there are a large number of arabinose and xylose linkages in the wall, and GAX is significantly branched at the developmental stage of 2 weeks. 2D (13)C-(13)C correlation spectra measured with long spin diffusion mixing times indicate that the branched GAX approaches cellulose microfibrils on the nanometer scale, contrary to the conventional model in which only unbranched GAX can bind cellulose. The GAX chains are highly dynamic, with average order parameters of ~0.4. Biexponential (13)C T1 and (1)H T1ρ relaxation indicates that there are two dynamically distinct domains in GAX: the more rigid domain may be responsible for cross-linking cellulose microfibrils, while the more mobile domain may fill the interfibrillar space. This dynamic heterogeneity is more pronounced than that of the non-grass hemicellulose, XyG, suggesting that GAX adopts the mixed characteristics of XyG and pectins. Moderate differences in cellulose rigidity are observed between the Brachypodium and Arabidopsis cell walls, suggesting different effects of the matrix polysaccharides on cellulose. These data provide the first molecular-level structural information about the three-dimensional organization of the polysaccharides in the grass primary wall.
Assuntos
Brachypodium/química , Parede Celular/química , Polissacarídeos/química , Isótopos de Carbono , Glucanos , Espectroscopia de Ressonância Magnética/métodos , XilanosRESUMO
This paper reports the identification of a barley UDP-glucosyltransferase, HvUGT14077, which is able to convert the estrogenic Fusarium mycotoxin zearalenone into a near-equimolar mixture of the known masked mycotoxin zearalenone-14-O-ß-glucoside and a new glucose conjugate, zearalenone-16-O-ß-glucoside. Biocatalytical production using engineered yeast expressing the HvUGT14077 gene allowed structural elucidation of this compound. The purified zearalenone-16-O-ß-glucoside was used as an analytical calibrant in zearalenone metabolization experiments. This study confirmed the formation of this new masked mycotoxin in barley seedlings as well as in wheat and Brachypodium distachyon cell suspension cultures. In barley roots, up to 18-fold higher levels of zearalenone-16-O-ß-glucoside compared to the known zearalenone-14-O-ß-glucoside were found. Incubation of zearalenone-16-O-ß-glucoside with human fecal slurry showed that this conjugate can also be hydrolyzed rapidly by intestinal bacteria, converting the glucoside back to the parental mycotoxin. Consequently, it should be considered as an additional masked form of zearalenone with potential relevance for food safety.
Assuntos
Brachypodium/metabolismo , Glucosídeos/metabolismo , Hordeum/metabolismo , Micotoxinas/metabolismo , Triticum/metabolismo , Zearalenona/química , Zearalenona/metabolismo , Adulto , Bactérias/metabolismo , Brachypodium/química , Feminino , Contaminação de Alimentos , Glucosídeos/química , Glucosiltransferases/genética , Glucosiltransferases/metabolismo , Hordeum/química , Hordeum/enzimologia , Hordeum/genética , Humanos , Mucosa Intestinal/metabolismo , Intestinos/microbiologia , Masculino , Microbiota , Micotoxinas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Triticum/químicaRESUMO
Polysaccharides make up about 75% of plant cell walls and can be broken down to produce sugar substrates (saccharification) from which a whole range of products can be obtained, including bioethanol. Cell walls also contain 5-10% of proteins, which could be used to tailor them for agroindustrial uses. Here we present cell wall proteomics data of Brachypodium distachyon, a model plant for temperate grasses. Leaves and culms were analyzed during active growth and at mature stage. Altogether, 559 proteins were identified by LC-MS/MS and bioinformatics, among which 314 have predicted signal peptides. Sixty-three proteins were shared by two organs at two developmental stages where they could play housekeeping functions. Differences were observed between organs and stages of development, especially at the level of glycoside hydrolases and oxidoreductases. Differences were also found between the known cell wall proteomes of B. distachyon, Oryza sativa, and the Arabidopsis thaliana dicot. Three glycoside hydrolases could be immunolocalized in cell walls using polyclonal antibodies against proteotypic peptides. Organ-specific expression consistent with proteomics results could be observed as well as cell-specific localization. Moreover, the high number of proteins of unknown function in B. distachyon cell wall proteomes opens new fields of research for monocot cell walls.
Assuntos
Biocombustíveis , Brachypodium/química , Parede Celular/química , Proteínas de Plantas/análise , Proteoma/análise , Brachypodium/citologia , Glicosídeo Hidrolases/análise , Glicosídeo Hidrolases/química , Imuno-Histoquímica , Folhas de Planta/química , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Caules de Planta/química , Proteoma/química , Proteoma/metabolismoRESUMO
Epitope databases and the protein sequences of published plant genomes are suitable to identify some of the proteins causing food allergies and sensitivities. Brachypodium distachyon, a diploid wild grass with a sequenced genome and low prolamin content, is the closest relative of the allergen cereals, such as wheat or barley. Using the Brachypodium genome sequence, a workflow has been developed to identify potentially harmful proteins which may cause either celiac disease or wheat allergy-related symptoms. Seed tissue-specific expression of the potential allergens has been determined, and intact epitopes following an in silico digestion with several endopeptidases have been identified. Molecular function of allergen proteins has been evaluated using Gene Ontology terms. Biologically overrepresented proteins and potentially allergen protein families have been identified.