RESUMO
Membrane lipid remodeling contributes to the environmental acclimation of plants. In the green lineage, a betaine lipid, diacylglyceryl-N,N,N-trimethylhomoserine (DGTS), is included exclusively among green algae and nonflowering plants. Here, we show that the green alga Chlorella kessleri synthesizes DGTS under phosphorus-deficient conditions through the eukaryotic pathway via the ER. Simultaneously, phosphatidylcholine and phosphatidylethanolamine, which are similar to DGTS in their zwitterionic properties, are almost completely degraded to release 18.1% cellular phosphorus, and to provide diacylglycerol moieties for a part of DGTS synthesis. This lipid remodeling system that substitutes DGTS for extrachloroplast phospholipids to lower the P-quota operates through the expression induction of the BTA1 gene. Investigation of this lipid remodeling system is necessary in a wide range of lower green plants for a comprehensive understanding of their phosphorus deficiency acclimation strategies.
Assuntos
Chlorella , Lipídeos de Membrana , Triglicerídeos , Membrana Celular/química , Membrana Celular/metabolismo , Membrana Celular/fisiologia , Chlorella/citologia , Chlorella/metabolismo , Chlorella/fisiologia , Lipídeos de Membrana/química , Lipídeos de Membrana/metabolismo , Fosfolipídeos/química , Fosfolipídeos/metabolismo , Estresse Fisiológico/fisiologia , Triglicerídeos/química , Triglicerídeos/metabolismoRESUMO
The judgment of microalgae viability is a vital procedure in the process of microalgae culture and treatment, which also plays an important role in bioremediation, bioindication, and pharmacology fields. The current conventional methods for defining living/dead microalgal cells are complicated or laborious. Hence, developing a simple and reliable detection method for microalgae viability is still challenging. Here, we developed chlorella-based carbonized polymer dots (c-CPDs) by a hydrothermal method. Due to their small average size of 5.0 nm, obvious excitation-dependent emission, stable fluorescence properties, and low toxicity, c-CPDs could be used for distinguishing living or dead chlorella by testing different fluorescence characteristics of c-CPD-labeled chlorella. Compared with conventional cellular dyes used for differentiating living/dead microalgae, c-CPDs significantly reduced toxicity, showing good sensitivity and reliability. This work provided a method to prepare environmentally friendly carbon dots (CDs) using microalgae, which had potential to be prepared on a large scale and might be applied feasibly in the preparation of doped CDs by controlling the growth of chlorella.
Assuntos
Carbono/química , Chlorella/química , Pontos Quânticos/química , Chlorella/citologia , Chlorella/crescimento & desenvolvimento , Corantes Fluorescentes/química , Concentração de Íons de Hidrogênio , Microalgas/efeitos dos fármacos , Microalgas/fisiologia , Polímeros/química , Pontos Quânticos/toxicidadeRESUMO
MAIN CONCLUSION: The supramolecular organization of the photosystem supercomplexes in the green alga Chlorella sorokiniana belonging to Trebouxiophyceae are essentially the same as those of Chlamydomonas reinhardtii belonging to Chlorophyceae. The photosynthetic conversion of light energy into chemical energy is performed by photosystems II and I (PSII and PSI) embedded within the thylakoid membranes. In plants and green algae, PSII and PSI comprise the core complex and light-harvesting complexes (LHCII and LHCI), forming PSII-LHCII and PSI-LHCI supercomplexes, respectively. The structural information about photosystem supercomplexes of green algae has been limited to chlorophytic algae. Here, to obtain an insight into the evolution of Chlorophyta, we determined the supramolecular organization of the PSII-LHCII and PSI-LHCI supercomplexes from the freshwater green alga Chlorella sorokiniana, which belongs to Trebouxiophyceae. The obtained results showed that the supramolecular organizations of the photosystem supercomplexes in C. sorokiniana were essentially the same as those of the model green alga C. reinhardtii, which belongs to Chlorophyceae, namely PSII-LHCII supercomplex formed the C2S2M2L2 configuration and PSI-LHCI supercomplex was associated with 10 LHCI subunits.
Assuntos
Chlorella , Complexo de Proteína do Fotossistema I , Complexo de Proteína do Fotossistema II , Chlorella/citologia , Chlorella/fisiologia , Evolução Molecular , Complexo de Proteína do Fotossistema I/metabolismo , Complexo de Proteína do Fotossistema II/metabolismo , Tilacoides/metabolismoRESUMO
Anaerobic digestion of microalgal biomass for biogas production may be limited due to the cell wall resulting in an inefficient bioconversion. Enzymatic pretreatments are applied for inducing cell damage/lysis and organic matter solubilisation and this way increasing biogas production. We evaluated enzymatic pretreatments in different conditions for comparing in relation to cell wall rupture, increase of soluble material and increase in biogas production through anaerobic digestion performance in BMP assay. Chlorella sorokiniana cultures were subjected to three different enzymatic pretreatments, each under four different conditions of enzyme/substrate ratio, pH and application time. The results showed increases over 21% in biogas productions for all enzymatic pretreatments. Enzymatic pretreatment was effective at damaging microalgae cell wall, releasing organic compounds and increasing the rate and final methane yield in BMP tests. We observed a synergistic activity between the mixtures enzymes, which would depend on operational conditions used for each pretreatment.
Assuntos
Biomassa , Biotecnologia/métodos , Parede Celular/metabolismo , Celulase/metabolismo , Chlorella/citologia , Metano/biossíntese , Microalgas/citologia , Anaerobiose , Chlorella/metabolismo , Microalgas/metabolismo , SolubilidadeRESUMO
The frequent occurrence of algal blooms in surface water has attracted more and more attention, which caused many water quality problems, including disinfection byproducts (DBPs). Algal organic matter (AOM) including intracellular organic matter (IOM) and extracellular organic matter (EOM), was a well-known precursor to DBPs formation in drinking water. This study evaluated the effect of ultraviolet (UV) irradiation on the cell integrity, IOM release and DBPs formation during subsequent chlorination of Chlorella sp. Results showed the damage rates of algal cells increased to 40.1% after the high UV irradiation of 528â¯mJ/cm2, which contributed to the release of IOM. In addition, UV irradiation was effective in reducing the formation of haloacetic acids (HAAs) both in AOM and IOM, but promoted the formation of nitrogenous DBPs (N-DBPs) from AOM in subsequent chlorination. Furthermore, neutral pH exerted a positive effect on the formation of DBPs. UV irradiation decreased the bromine substitution factor (BSF) value of AOM at a high bromide level. The BSF values increased with increasing of the concentration of bromide. Moreover, more amino acids and low molecular weight precursors were produced after UV irradiation in filtered supernatant, which contributed to the formation of N-DBPs with algal chlorination. Overall, this information demonstrated pre-oxidation of UV irradiation could be used to treat the algal-rich drinking water.
Assuntos
Chlorella/efeitos da radiação , Cloro/química , Desinfetantes/efeitos da radiação , Raios Ultravioleta , Poluentes Químicos da Água/efeitos da radiação , Purificação da Água , Chlorella/citologia , Chlorella/fisiologia , Desinfetantes/análise , Halogenação , Poluentes Químicos da Água/análise , Purificação da Água/instrumentaçãoRESUMO
The combined effect of polystyrene (PS) particles and triphenyltin chloride (TPTCl) to the green algae Chlorella pyrenoidosa was studied. The 96 h IC50 of TPTCl to the green algae C. pyrenoidosa was 30.64 µg/L. The toxicity of PS particles to C. pyrenoidosa was size-dependent, with the 96 h IC50 at 9.10 mg/L for 0.55 µm PS but no toxicity observed for 5.0 µm PS. The exposure to 0.55 µm PS led to damage on structure of algal cells, which could in turn cause inhibition on photosynthesis and population growth of the green algae. TPTCl concentrations in test medium were lowered by 15-19% at presence of 0.55 µm PS particles, indicating a reduced bioavailability of TPTCl. In spite of this reduced bioavailability, the presence of PS increased the toxicity of TPTCl, which might be attributed to facilitated uptake of TPTCl by the green algae after the damage of cell structure. The overall results of the present study provided important information on the effect of PS on the bioavailability and toxicity of TPTCl to phytoplankton species.
Assuntos
Chlorella/efeitos dos fármacos , Compostos Orgânicos de Estanho/toxicidade , Poliestirenos/toxicidade , Poluentes Químicos da Água/toxicidade , Disponibilidade Biológica , Chlorella/citologia , Chlorella/fisiologia , Ecotoxicologia/métodos , Compostos Orgânicos de Estanho/farmacocinética , Fotossíntese/efeitos dos fármacos , Fitoplâncton/efeitos dos fármacosRESUMO
A mild and low-energy cell disruption method with high efficiency has growing application potential in both the extraction of high-value microalgal products and the inactivation of microalgal cells. Conventional technologies available have disadvantages including high energy consumption, the use of chemicals and so on. Here, this study developed an efficient microalgal cell disruption method using the copper oxide nanowire (CuONW)-modified three-dimensional (3D) copper foam electrodes with a low applied voltage. Electrodes with nanowires synthesized at 400⯰C, the optimal preparation temperature, achieved efficient microalgal cell electroporation. Microalgal cells were completely inactivated and disrupted at the voltage of 2â¯V with the hydraulic retention time (HRT) of 10â¯s. Scanning electron microscopy (SEM) images showed obvious electroporation damage on the cell surface upon electroporation-treatment (2â¯V, 30â¯s). The amount of released cellular inclusion increased significantly with prolonged HRT and the energy consumption of this technology was only 0.014â¯kWh/kg via the treatment of 2â¯V and 10â¯s. This study provided a novel, energy-efficient and chemical-free technique for both microalgal products extraction and cell inactivation.
Assuntos
Técnicas de Cultura de Células/métodos , Chlorella/fisiologia , Corpos de Inclusão/fisiologia , Microalgas/fisiologia , Nanofios/química , Chlorella/citologia , Chlorella/crescimento & desenvolvimento , Eletrodos , Eletroporação , Microalgas/citologia , Microalgas/crescimento & desenvolvimentoRESUMO
Thorium (Th) is a natural radioactive element present in the environment and has the potential to be used as a clean nuclear fuel. Relatively little is known about the aquatic toxicity of Th, especially in nanoparticulate form, which may be the main chemical species of Th in the natural waters. In this study, impacts of ThO2 nanoparticles (NPs) with two different sizes (52 ± 5 nm, s-ThO2vs. 141 ± 6 nm, b-ThO2) on a green alga Chlorella pyrenoidosa (C. pyrenoidosa) were evaluated. Results indicated that C. pyrenoidosa was more sensitive to s-ThO2 (96-h EC30 = 64.1 µM) than b-ThO2 (96-h EC30 = 100.2 µM). Exposure to 200 µM of ThO2 NPs reduced the chlorophyll-a and chlorophyll-b contents of the algal cells. At 96 h, SEM and TEM showed that more agglomerates of s-ThO2 than those of b-ThO2 were attached onto the surface of algal cells. Reactive oxygen species (ROS) generation and membrane damage were induced after the attachment of high concentrations of ThO2 NPs. The heteroagglomeration between ThO2 NPs and algal cells and increased oxidative stress might play important roles in the toxicity of ThO2 NPs. To the best of our knowledge, this is the first report on aquatic toxicity of ThO2 NPs.
Assuntos
Chlorella/efeitos dos fármacos , Nanopartículas/toxicidade , Tamanho da Partícula , Dióxido de Tório/toxicidade , Testes de Toxicidade , Permeabilidade da Membrana Celular/efeitos dos fármacos , Chlorella/citologia , Chlorella/crescimento & desenvolvimento , Chlorella/ultraestrutura , Clorofila/análise , Clorofila A/análise , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Poluentes Químicos da Água/toxicidadeRESUMO
Organophosphorus compounds exhibit a wide range of toxicity to mammals. In this study the effect of malathion on the growth and biochemical parameters of microalgae was evaluated. Three microalgae (Micractinium pusillum UUIND2, Chlorella singulari UUIND5 and Chlorella sorokiniana UUIND6) were used in this study. Among the three algal strains tested, Chlorella sorokiniana UUIND6 was able to tolerate 100 ppm of malathion. The photosynthetic pigments, the protein, carbohydrate and lipid contents of microalgal cells were also analyzed. About 90% degradation was recorded in 25 ppm, 50 ppm and 70% was recorded in 100 ppm of malathion by Chlorella sorokiniana. A mechanism of degradation of malathion by Chlorella sorokiniana is proposed in this study. Activity of carboxylesterase was increased in algal cells cultivated in malathion containing medium which confirmed that malathion degraded into phosphate. Increased amount of Malondialdehye (MDA) indicate the development of free radicals under the stress of malathion which substantialy increase de novo TAG biosynthesis, while increased level of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) suggested their association in scavenging of free radical.
Assuntos
Carboxilesterase/metabolismo , Microalgas/metabolismo , Compostos Organofosforados/toxicidade , Praguicidas/toxicidade , Triglicerídeos/biossíntese , Biocombustíveis , Biomassa , Tamanho Celular , Chlorella/citologia , Chlorella/efeitos dos fármacos , Chlorella/metabolismo , Inativação Metabólica/efeitos dos fármacos , Malation/toxicidade , Microalgas/citologia , Microalgas/efeitos dos fármacos , Fotossíntese/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Testes de Toxicidade , Poluentes Químicos da Água/toxicidadeRESUMO
There has been a developing technology in algae with pharmaceuticals wastewater. However, the effect and the underlying mechanism of pharmaceuticals on algae are not well understood. To investigate the effect and mechanism of pharmaceuticalson microalgae, four pharmaceuticals of clofibric acid (CLF), ciprofloxacin (CIP), diclofenac (DCF) and carbamazepine (CBZ) on C. pyrenoidosa culture were analyzed. At low concentrations (<10 mg/L), the pharmaceuticals, especially the DCF, exhibited positive effects on both the structure and function of algal cultures; algal growth (i.e., chlorophyll a accumulation, lipid accumulation) and activities of antioxidant enzymes were stimulated. The algal metabolite differences of various DCF concentrations were investigated and a total of 91 substances were identified, whose samples were clustered and clearly separated. The key metabolomics pathway analysis found that the DCF promoted the carbohydrate and fatty acid metabolic pathway in C. pyrenoidosa under relatively low concentrations (<10 mg/L). However, the algae metabolomics pathway was disturbed significantly under the action of a high concentration of DCF (>100 mg/L). The study detected the effects of four pharmaceuticals on C. pyrenoidosa and demonstrated that the usage of metabolomics analysis complemented with DCF could be an effective approach to understand the mechanism of molecular evolution in C. pyrenoidosa for microalgal biomass and bioenergy from wastewater in researches of biological resources.
Assuntos
Chlorella/efeitos dos fármacos , Chlorella/crescimento & desenvolvimento , Chlorella/metabolismo , Preparações Farmacêuticas , Carbamazepina/farmacologia , Catalase/metabolismo , Técnicas de Cultura de Células , Chlorella/citologia , Clorofila A/metabolismo , Ciprofloxacina/farmacologia , Ácido Clofíbrico/farmacologia , Diclofenaco/farmacologia , Relação Dose-Resposta a Droga , Metabolismo dos Lipídeos/efeitos dos fármacos , Metabolômica , Microalgas/efeitos dos fármacos , Tamanho da Partícula , Superóxido Dismutase/metabolismoRESUMO
Astaxanthin represents a high-value ketocarotenoid that has been gaining great attention from both the science and public communities in recent years. Microalgae are the primary source of natural astaxanthin. Chlorella zofingiensis, a freshwater and oleaginous green microalga, is capable of growing well photoautotrophically, heterotrophically, and mixotrophically for high-density biomass and astaxanthin production. Here we describe the astaxanthin production pipeline using C. zofingiensis as cell factories under different trophic conditions, including strain preparation, inoculation, and cultivation, biomass harvest and dewatering, and astaxanthin extraction, determination, and quantification.
Assuntos
Chlorella/química , Chlorella/crescimento & desenvolvimento , Processos Autotróficos , Biomassa , Chlorella/citologia , Processos Heterotróficos , Técnicas Microbiológicas , Estrutura Molecular , Análise Espectral , Xantofilas/análise , Xantofilas/químicaRESUMO
Microalgae biotechnologies are rapidly developing into new commercial settings. Several high value products already exist on the market, and systems development is focused on cost reduction to open up future economic opportunities for food, fuel and freshwater production. Light is a key environmental driver for photosynthesis and optimising light capture is therefore critical for low cost, high efficiency systems. Here a novel high-throughput screen that simulates fluctuating light regimes in mass cultures is presented. The data was used to model photosynthetic efficiency (PEµ, mol photon-1 m2) and chlorophyll fluorescence of two green algae, Chlamydomonas reinhardtii and Chlorella sp. Response surface methodology defined the effect of three key variables: density factor (Df, 'culture density'), cycle time (tc, 'mixing rate'), and maximum incident irradiance (Imax). Both species exhibited a large rise in PEµ with decreasing Imax and a minimal effect of tc (between 3-20 s). However, the optimal Df of 0.4 for Chlamydomonas and 0.8 for Chlorella suggested strong preferences for dilute and dense cultures respectively. Chlorella had a two-fold higher optimised PEµ than Chlamydomonas, despite its higher light sensitivity. These results demonstrate species-specific light preferences within the green algae clade. Our high-throughput screen enables rapid strain selection and process optimisation.
Assuntos
Biotecnologia/métodos , Luz , Microalgas/efeitos da radiação , Células Cultivadas , Chlamydomonas reinhardtii/citologia , Chlamydomonas reinhardtii/efeitos da radiação , Chlorella/citologia , Chlorella/efeitos da radiação , Microalgas/citologia , Fotossíntese/efeitos da radiação , Complexo de Proteína do Fotossistema II/metabolismoRESUMO
Heavy ion beams have many exciting applications, including radiotherapy of deep-seated tumors and simulation tests of space irradiation for astronauts. These beams often use a feature that concentrates the energy deposition largely along the end of the energy pathway, leading to different distributions of biological effects along the axial direction. Currently, there is relatively little information regarding the radial directional difference of biological effects along the heavy ion paths. This study utilized a filter membrane that was quantatively applied with cells to demonstrate a 3D distribution model of irradiation on biological effects in living organisms. Some results have indicated that there is excitatory effect on the non-irradiated regions with energetic ions, which may give new insights into the distribution of biological effects along the paths of heavy ion beams with mid-high energy.
Assuntos
Carbono/química , Íons Pesados , Imageamento Tridimensional , Chlorella/citologia , Chlorella/efeitos da radiação , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/efeitos da radiação , Superóxidos/metabolismoRESUMO
BACKGROUND: Microalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show efficiency limitation and until now, extraction protocols have not been fully optimized for specific lipid compounds. The present study thus presents a novel lipid extraction method, consisting in the addition of a water treatment of biomass between the two-stage solvent extraction steps of current extraction methods. The resulting modified method not only enhances lipid extraction efficiency, but also yields a higher triacylglycerols (TAG) ratio, which is highly desirable for biodiesel production. RESULTS: Modification of four existing methods using acetone, chloroform/methanol (Chl/Met), chloroform/methanol/H2O (Chl/Met/H2O) and dichloromethane/methanol (Dic/Met) showed respective lipid extraction yield enhancement of 72.3, 35.8, 60.3 and 60.9%. The modified acetone method resulted in the highest extraction yield, with 68.9 ± 0.2% DW total lipids. Extraction of TAG was particularly improved with the water treatment, especially for the Chl/Met/H2O and Dic/Met methods. The acetone method with the water treatment led to the highest extraction level of TAG with 73.7 ± 7.3 µg/mg DW, which is 130.8 ± 10.6% higher than the maximum value obtained for the four classical methods (31.9 ± 4.6 µg/mg DW). Interestingly, the water treatment preferentially improved the extraction of intracellular fractions, i.e. TAG, sterols, and free fatty acids, compared to the lipid fractions of the cell membranes, which are constituted of phospholipids (PL), acetone mobile polar lipids and hydrocarbons. Finally, from the 32 fatty acids analyzed for both neutral lipids (NL) and polar lipids (PL) fractions, it is clear that the water treatment greatly improves NL-to-PL ratio for the four standard methods assessed. CONCLUSION: Water treatment of biomass after the first solvent extraction step helps the subsequent release of intracellular lipids in the second extraction step, thus improving the global lipids extraction yield. In addition, the water treatment positively modifies the intracellular lipid class ratios of the final extract, in which TAG ratio is significantly increased without changes in the fatty acids composition. The novel method thus provides an efficient way to improve lipid extraction yield of existing methods, as well as selectively favoring TAG, a lipid of the upmost interest for biodiesel production.
Assuntos
Chlorella/química , Lipídeos/isolamento & purificação , Extração Líquido-Líquido/métodos , Triglicerídeos/análise , Biocombustíveis , Biomassa , Fracionamento Celular/métodos , Chlorella/citologia , Ácidos Graxos/análise , Ácidos Graxos/química , Ácidos Graxos/isolamento & purificação , Lipídeos/análise , Lipídeos/química , Metanol , Solventes , Triglicerídeos/isolamento & purificação , ÁguaRESUMO
OBJECTIVE: To fabricate a novel microbial photobioelectrochemical cell using silicon microfabrication techniques. RESULTS: High-density photosynthetic cells were immobilized in a microfluidic chamber, and ultra-microelectrodes in a microtip array were inserted into the cytosolic space of the cells to directly harvest photosynthetic electrons. In this way, the microbial photobioelectrochemical cell operated without the aid of electron mediators. Both short circuit current and open circuit voltage of the microbial photobioelectrochemical cell responded to light stimuli, and recorded as high as 250 pA and 45 mV, respectively. CONCLUSION: A microbial photobioelectrochemical cell was fabricated with potential use in next-generation photosynthesis-based solar cells and sensors.
Assuntos
Fontes de Energia Bioelétrica , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Fotobiorreatores , Células Imobilizadas , Chlorella/citologia , Chlorella/metabolismo , Técnicas Eletroquímicas , Desenho de Equipamento , MicroeletrodosRESUMO
In this study, harvesting efficiency (HE) of bioflocculant (egg shell) was observed with variation in flocculent concentrations (0-100mgL-1), temperature (30°C, 35°C 40°C, 45°C and 50°C) and variable contact time (0-50min). It was found maximum (≈95.6%) with 100mgL-1 bioflocculant concentration whereas influence of temperature was also observed with optimized concentration of bioflocculant (100mgL-1) at 40°C (≈98.1%) and 50°C (≈99.3%), in 30min of contact time. Significant changes in algal cell structures were also analyzed after exposure to various temperatures with microscopy, SEM (Scanning electron microscopy) and EDS (Energy dispersive X-ray spectroscopy) images with and without bioflocculant. The experimental data was found to be a good fit with pseudo-second order kinetic model. The thermodynamic functions such as ΔG (Gibbs free energy), ΔH (enthalpy), ΔS (entropy) were also determined. The negative value of ΔG and positive value of ΔH and ΔS shows the spontaneous and endothermic nature of flocculation process.
Assuntos
Biotecnologia/métodos , Chlorella/química , Biotecnologia/economia , Chlorella/citologia , Chlorella/crescimento & desenvolvimento , Custos e Análise de Custo , Entropia , Floculação , Cinética , Microscopia Eletrônica de Varredura , Espectrometria por Raios X , Temperatura , TermodinâmicaRESUMO
Effective enhancement of neutral lipid (especially triacylglycerol, TAG) content in microalgae is an important issue for commercialization of microalgal biorefineries. Pressure is a key physical factor affecting the morphological, physiological, and biochemical behaviors of organisms. In this paper, we report a new stress-based method for induction of TAG accumulation in microalgae (specifically, Chlorella sp. KR-1 and Ch. sp. AG20150) by very-short-duration application of mild pressure. Pressure treatments of 10-15bar for 2h resulted in a considerable, â¼55% improvement of the 10-100g/Lcells' TAG contents compared with the untreated control. The post-pressure-treatment increase of cytoplasmic TAG granules was further confirmed by transmission electron microscopy (TEM). Notwithstanding the increased TAG content, the total lipid content was not changed by pressurization, implying that pressure stress possibly induces rapid remodeling/transformation of algal lipids rather than de novo biosynthesis of TAG.
Assuntos
Chlorella/metabolismo , Pressão , Triglicerídeos/biossíntese , Chlorella/citologia , Chlorella/efeitos dos fármacos , Microalgas/metabolismo , Fatores de TempoRESUMO
Endosymbioses are driving forces underlying cell evolution. The endosymbiosis exhibited by Paramecium bursaria is an excellent model with which to study symbiosis. A single-cell microscopic analysis of P. bursaria reveals that endosymbiont numbers double when the host is in the division phase. Consequently, endosymbionts must arrange their cell cycle schedule if the culture-condition-dependent change delays the generation time of P. bursaria. However, it remains poorly understood whether endosymbionts keep pace with the culture-condition-dependent behaviors of P. bursaria, or not. Using microscopy and flow cytometry, this study investigated the life cycle behaviors occurring between endosymbionts and the host. To establish a connection between the host cell cycle and endosymbionts comprehensively, multivariate analysis was applied. The multivariate analysis revealed important information related to regulation between the host and endosymbionts. Results show that dividing endosymbionts underwent transition smoothly from the division phase to interphase, when the host was in the logarithmic phase. In contrast, endosymbiont division stagnated when the host was in the stationary phase. This paper explains that endosymbionts fine-tune their cell cycle pace with their host and that a synchronous life cycle between the endosymbionts and the host is guaranteed in the symbiosis of P. bursaria.
Assuntos
Chlorella/fisiologia , Paramecium/microbiologia , Paramecium/fisiologia , Simbiose/fisiologia , Animais , Evolução Biológica , Ciclo Celular , Chlorella/citologia , Citometria de Fluxo/métodos , Estágios do Ciclo de Vida/fisiologia , Análise Multivariada , Paramecium/crescimento & desenvolvimentoRESUMO
The ability to image large numbers of cells at high resolution enhances flow cytometric analysis of cells and cell populations. In particular, the ability to image intracellular features adds a unique aspect to analyses, and can enable correlation between molecular phenomena resulting in alterations in cellular phenotype. Unicellular microalgae are amenable to high-throughput analysis to capture the diversity of cell types in natural samples, or diverse cellular responses in clonal populations, especially using imaging cytometry. Using examples from our laboratory, we review applications of imaging cytometry, specifically using an Amnis(®) ImageStream(®)X instrument, to characterize photosynthetic microalgae. Some of these examples highlight advantages of imaging flow cytometry for certain research objectives, but we also include examples that would not necessarily require imaging and could be performed on a conventional cytometer to demonstrate other concepts in cytometric evaluation of microalgae. We demonstrate the value of these approaches for (1) analysis of populations, (2) documentation of cellular features, and (3) analysis of gene expression.
Assuntos
Chlorella/citologia , Citometria de Fluxo/métodos , Citometria por Imagem/métodos , Microalgas/citologiaRESUMO
This work reports on an efficient microfluidic approach for continuous production of hollow Ca-alginate microfibers with controllable structures and functions. A coaxial microcapillary microfluidic device combined with a rotator is constructed to produce a cylindrical flow jet with four aqueous solutions as templates for continuous fabrication and collection of microfibers. A four-aqueous-phase flow jet with an intermediate buffer flow between the Ca(2+)-containing and alginate-containing flows is used as the template for microfiber fabrication. The buffer flow efficiently controls the diffusion of Ca(2+) into the alginate-containing flow as well as the crosslinking reaction, thus ensuring the continuous fabrication of hollow Ca-alginate microfibers under relatively low flow rates without clogging of the microchannel. The structure of the hollow microfibers can be flexibly adjusted by changing the flow rates and device dimensions. Meanwhile, the continuous fabrication process of the microfibers allows flexible incorporation of a functional component into the sheath flow for functionalization and addition of active substances in the core flow for encapsulation. This is demonstrated by fabricating hollow Ca-alginate microfibers with a wall containing magnetic nanoparticles for magnetic functionalization and with hollow internals containing Chlorella pyrenoidosa cells for confined growth. This work provides an efficient strategy for continuous fabrication of functional hollow Ca-alginate microfibers with controllable structures and functions.