Impact of heavy precipitation events on pathogen occurrence in estuarine areas of the Puzi River in Taiwan.

Pathogen populations in estuarine areas are dynamic, as they are subject to multiple natural and anthropogenic challenges. Heavy rainfall events bring instability to the aquatic environment in estuaries, causing changes in pathogen populations and increased environmental sanitation and public health concerns. In this study, we investigated the effects of heavy precipitation on the occurrence of pathogens in the Puzi River estuary, which is adjacent to the largest inshore oyster farming area in Taiwan. Our results indicated that Vibrio parahaemolyticus and adenovirus were the most frequently detected pathogens in the area. There was a significant difference (Mann-Whitney U test, p < 0.01) in water quality parameters, including total coliform, Escherichia coli, water temperature, turbidity, salinity, and dissolved oxygen, between groups with and without V. parahaemolyticus. In addition, the detection rate was negatively correlated with the average daily rainfall (r2 > 0.8). There was no significant difference between water quality parameters and the presence/absence of adenovirus, but a positive correlation was observed between the average daily rainfall and the detection rate of adenovirus (r2 ≥ 0.75). We conclude that heavy precipitation changes estuarine water quality, causing variations in microbial composition, including pathogens. As extreme weather events become more frequent due to climate change, the potential impacts of severe weather events on estuarine environments require further investigation.

Cyanobacteria and Algae in Clouds and Rain in the Area of puy de Dôme, Central France.

The atmosphere contains diverse living microbes, of which the heterotrophic community has been the best studied. Microbes with other trophic modes, such as photoautotrophy, have received much less attention. In this study, culture-independent and dependent methods were used to examine the presence and diversity of oxygenic photoautotrophic microbes in clouds and rain collected at or around puy de Dôme Mountain, central France. Cloud water was collected from the summit of puy de Dôme (1,465 m above sea level [a.s.l.]) for cultivation and metagenomic analysis. Cyanobacteria, diatoms, green algae, and other oxygenic photoautotrophs were found to be recurrent members of clouds, while green algae affiliated with the Chlorellaceae were successfully cultured from three different clouds. Additionally, rain samples were collected below the mountain from Opme meteorological station (680 m a.s.l.). The abundance of chlorophyll a-containing cells and the diversity of cyanobacteria and green algae in rain were assessed by flow cytometry and amplicon sequencing. The corresponding downward flux of chlorophyll a-containing organisms to the ground, entering surface ecosystems with rain, varied with time and was estimated to be between ∼1 and >300 cells cm-2 day-1 during the sampling period. Besides abundant pollen from Pinales and Rosales, cyanobacteria of the Chroococcidiopsidales and green algae of the Trebouxiales were dominant in rain samples. Certain members of these taxa are known to be ubiquitous and stress tolerant and could use the atmosphere for dispersal. Overall, our results indicate that the atmosphere carries diverse, viable oxygenic photoautotrophic microbes and acts as a dispersal vector for this microbial guild.IMPORTANCE Information regarding the diversity and abundance of oxygenic photoautotrophs in the atmosphere is limited. More information from diverse locations is needed. These airborne organisms could have important impacts upon atmospheric processes and on the ecosystems they enter after deposition. Oxygenic photoautotrophic microbes are integral to ecosystem functioning, and some have the potential to affect human health. A better understanding of the diversity and the movements of these aeolian dispersed organisms is needed to understand their ecology, as well as how they could affect ecosystems and human health.

Inoculum quantification of canker-causing pathogens in prune and walnut orchards using real-time PCR.

AIMS: A real-time quantitative PCR (qPCR) assay was established to quantify the inoculum densities in the air and rainwater for six canker-causing pathogen groups in prune and walnut orchards in California. METHODS AND RESULTS: The previously published DNA primers to target six pathogen groups including Botryosphaeria dothidea, Cytospora spp., Diplodia spp., Lasiodiplodia spp., Neofusicoccum spp. and Phomopsis spp. were used in a qPCR assay. Air samples from Burkard spore traps and rain samples from special rain collector devices were collected periodically from various prune and walnut orchards. Using the qPCR approach, we were able to quantify the concentrations of these pathogen groups in rainwater and air samples and study the dynamics of pathogen inoculum in orchards showing severe canker potential. Phomopsis spp. and Diplodia spp. were not found in all rain samples in prune orchards, although they were detected in the 2016 in the walnut orchard. The other four pathogen groups were quantified at varying concentrations in the prune and walnut orchards. Cytospora spp. in some cases showed higher concentrations in the rainwater in prune orchards. CONCLUSIONS: The rainy season during winter and early spring is a highly risky period of time for infection by the pathogens when the inoculum of these pathogens can easily spread by air and rain water, thus serving as an important inoculum source for disease initiation. The different studied pathogen groups showed different concentrations during the growing season, indicating the complexity of the components of canker-causing species in various tree crops. SIGNIFICANCE AND IMPACT OF THE STUDY: This study showed the applicability of the qPCR assay in the quantification of inoculum in tree orchards to help reveal the mechanisms of canker disease epidemics and to help design disease management strategies.

Rain induces temporary shifts in epiphytic bacterial communities of cucumber and tomato fruit.

Understanding weather-related drivers of crop plant-microbiome relationships is important for food security and food safety in the face of a changing climate. Cucumber and tomato are commercially important commodities that are susceptible to plant disease and have been implicated in foodborne disease outbreaks. To investigate the influence of precipitation on plant-associated microbiomes, epiphytically associated bacterial communities of cucumber and tomato samples were profiled by 16 S rRNA gene sequencing (V1-V3) in the days surrounding two rain events over a 17-day period. Following rain, α (within-sample) diversity measured on cucumber and tomato fruit surfaces, but not tomato leaf surfaces, increased significantly and remained elevated for several days. Bacterial ß (between-sample) diversity on cucumber and tomato fruit responded to precipitation. In the cucumber fruit surface (carpoplane), notable shifts in the families Xanthomonadaceae, Oxalobacteriaceae, Sphingobacteriaceae and Comamonadaceae were detected following precipitation. In the tomato carpoplane, shifts were detected in the families Enterobacteriaceae and Xanthomonadaceae following the first rain event, and in the Pseudomonadaceae and Oxalobacteriaceae following the second rain event. Few taxonomic shifts were detected in the tomato leaf surface (phylloplane). Exploring rain-induced shifts in plant microbiomes is highly relevant to crop protection, food safety and agroecology, and can aid in devising ways to enhance crop resilience to stresses and climate fluctuations.

Bacterial adaptation is constrained in complex communities.

A major unresolved question is how bacteria living in complex communities respond to environmental changes. In communities, biotic interactions may either facilitate or constrain evolution depending on whether the interactions expand or contract the range of ecological opportunities. A fundamental challenge is to understand how the surrounding biotic community modifies evolutionary trajectories as species adapt to novel environmental conditions. Here we show that community context can dramatically alter evolutionary dynamics using a novel approach that 'cages' individual focal strains within complex communities. We find that evolution of focal bacterial strains depends on properties both of the focal strain and of the surrounding community. In particular, there is a stronger evolutionary response in low-diversity communities, and when the focal species have a larger genome and are initially poorly adapted. We see how community context affects resource usage and detect genetic changes involved in carbon metabolism and inter-specific interaction. The findings demonstrate that adaptation to new environmental conditions should be investigated in the context of interspecific interactions.

Survival of Brenneria goodwinii and Gibbsiella quercinecans, associated with acute oak decline, in rainwater and forest soil.

Acute oak decline (AOD) affects native UK oak species causing rapid decline and mortality in as little as five years. A major symptom of AOD is black weeping stem lesions associated with bacterial phytopathogens, Brenneria goodwinii and Gibbsiella quercinecans. However, there is limited knowledge on the ecological and environmental reservoirs of these phytopathogens. Rainwater and soils are common reservoirs of plant pathogens in a forest environment; therefore, the aim of this study was to investigate the survival of B. goodwinii and G. quercinecans in vitro when inoculated into rainwater and forest soil using a combination of agar-based colony counts and gyrB gene-targeted quantitative PCR (qPCR). Brenneria goodwinii lost viability on inoculation into soil and rainwater, but was detectable at low abundance in soil for 28 days using qPCR, suggesting a limited ability to persist outside of the host, potentially in a viable but non-culturable (VBNC) state. Conversely, Gibbsiella quercinecans, was re-isolated from rainwater for the entire duration of the experiment (84 days) and was re-isolated from forest soil after 28 days, with qPCR analysis corroborating these trends. These data demonstrate that B. goodwinii is unable to survive in forest soils and rainwater, suggesting that it may be an endosymbiont of oak trees, whereas G. quercinecans remains viable in soil and rainwater biomes, suggesting a broad ecological distribution. These data advance understanding of the potential epidemiology of AOD-associated bacteria and their ecological reservoirs, thus increasing the overall knowledge of the pathology of AOD, which assists the development of future management strategies.

Airborne bacterial communities and antibiotic resistance gene dynamics in PM2.5 during rainfall.

The biotoxicity and public health effects of airborne bacteria and antibiotic resistance genes (ARGs) in fine particulate matter (PM2.5) are being increasingly recognized. The characteristics of bacterial community composition and ARGs in PM2.5 under different rainfall conditions were studied based on the on-site synchronous measurements in downtown Beijing. Marked differences were evident in the bacterial community characteristics of PM2.5 before, during, and after rain events (p < 0.05). The rain intensities affected the bacterial community abundance in PM2.5 and heavy rain had greater washing effects. The Proteobacteria (phylum level), α-Proteobacteria (class level), Pseudomonadales (order level), Pseudomonadaceae (family level), and Cyanobacteria (genus level) were the dominant bacterial taxa associated with PM2.5 in Beijing during rain events. However, the bacteria at each level that displayed the biggest percentage variance was not the dominant type under different rain intensities. The ermB, tetW, and mphE genes were the primary ARGs, with abundances of 18 to 30 copies/m3, which was a relatively smaller value than other observations. Real-time monitoring of the meteorological condition of rain events and physicochemical properties of PM2.5 were used to identify the main factors during rainfall. The bacterial community was sensitive to the ionic and metal element components of PM2.5 during rainfall. The abundance of ARGs was closely correlated with some groups of the bacterial community, which were also close to the initial value before the rain. Statistical analysis demonstrated that temperature, relative humidity, and duration of rain were the primary meteorological factors for the biological characteristics. The ionic species, rather than metal elements, in PM2.5 were the sensitive factors for the bacteria community and ARGs, which varied at the phylum, class, order, family, and genus levels. The observations provide insights for the biological risk assessment in an urban rainfall water and the potential health impact on citizens.

Effect of temperature on opportunistic pathogen gene markers and microbial communities in long-term stored roof-harvested rainwater.

Roof-harvested rainwater (RHRW) has received increasing attention in recent years as an alternative water source for domestic use, yet its biological stability during storage is not fully understood. This study investigated the effects of temperature (4 °C, 20 °C and 30 °C) on the microbiological characteristics of RHRW over a storage period of 60 days by targeting different microbial groups including total bacteria and fecal indictor Escherichia coli, bacterial opportunistic pathogen genera and species (Legionella spp, Legionella pneumophila, Mycobacterium spp, Mycobacterium avium, Pseudomonas aeruginosa), and two amoebas (Acanthamoeba and Vermamoeba vermiformis). The rainwater chemistry demonstrated no obvious change during storage. The highest biomass was observed in RHRW stored at 30 °C, as measured by heterotrophic bacterial counts, adenosine triphosphate, and 16S rRNA gene numbers. Gene markers of E. coli, Legionella spp., P. aeruginosa, and V. vermiformis were detected in fresh RHRW and can persist during RHRW storage; whereas P. aeruginosa was the only species demonstrated significant regrowth at higher storage temperatures (P < 0.05). Acanthamoeba spp. was only detected in RHRW after 50 days of storage at three investigated temperatures, highlighting increased health risks in long-term stored RHRW. Bacterial community compositions were significantly different in RHRW stored at different temperatures, with increased variations among triplicate storage bottles noted at higher temperatures along with storage time. The results provide insights into RHRW storage practices in terms of mitigating microbial contamination risks.

Diversidad de especies de micobacterias no tuberculosas aisladas en ambientes acuáticos de la ciudad de General Pico, La Pampa, Argentina / Species diversity of non-tuberculous mycobacteria isolated from aquatic environments of General Pico city, Province of La Pampa (Argentina)

Las micobacterias no tuberculosas (MNT) no solo se estudian por su importancia como patógenos oportunistas, sino también por sus aplicaciones en biotecnología y biorremediación. Nuestro objetivo fue determinar la presencia de micobacterias en los distintos hábitats acuáticos de la ciudad de General Pico (provincia de La Pampa), así como su diversidad. Los porcentajes de muestras positivas a micobacterias fueron los siguientes: 37,5% en el sistema de distribución de agua de red, 32,6% en el acuífero que abastece dicho sistema, 36,8% en el agua proveniente de las precipitaciones, 53,1% en los humedales del área de influencia, 80% en los natatorios cubiertos y 33,3% en las fuentes decorativas ubicadas en plazas públicas. De los 90 aislamientos de MNT obtenidos el 8,9% no logró ser identificado a nivel de especie con los métodos utilizados, que incluyeron pruebas fenotípicas y métodos moleculares. Las especies más frecuentemente aisladas fueron Mycobacterium fortuitum y Mycobacterium gordonae. Algunas especies identificadas han sido reportadas en casos de micobacteriosis en nuestro país, entre ellas M. fortuitum, M. gordonae, M. intracellulare, M. vaccae, M. lentiflavum y M. nonchromogenicum. No se aislaron MNT en muestras de agua de red con concentraciones de cloro activo residual mayores de 0,8mg/l, mientras que en los natatorios la presencia de hasta 1,5mg/l de cloro activo residual no fue una limitante para la proliferación de estos microorganismos. Se puede considerar que la incidencia de micobacterias en los ambientes acuáticos de General Pico es cercana al 35%, y que la presencia de estos microorganismos y su diversidad se ve afectada por el contacto con el hombre y sus actividades, como así también por la existencia de vida animal.

Non-tuberculous mycobacteria (NTM) are studied not only for their importance as emerging opportunistic pathogens but also for their applications in biotechnology and bioremediation. Our aim was to determine the occurrence and diversity of mycobacteria in different aquatic habitats of General Pico city, Province of La Pampa. The percentage of samples with positive cultures for mycobacteria were the following: 37.5% recovered from the water supply distribution system; 32.6% from the aquifer that supplies water to the distribution system; 36.8% from rain water; 53.1% from the two wetlands in the area of influence; 80% from indoor swimming pools; and 33.3% from water fountains in downtown public squares. Of the 90 NTM isolates, 8.9% could not be identified at the species level with any of the used methods, phenotypic tests and molecular methods. Mycobacterium fortuitum and Mycobacterium gordonae were the most frequently isolated species. Some of the identified species such as, M. fortuitum, M. gordonae, M. intracellulare, M. vaccae, M. lentiflavum and M. nonchromogenicum, have been reported in cases of mycobacteriosis in Argentina. Mycobacteria with values higher than 0.8mg/ml of residual active chlorine were not recovered from the drinking water supply network, whereas in the swimming pools the presence of up to 1.5 mg/l was not a constraint. Based on our results, the presence of mycobacteria in aquatic environments is close to 35% and their occurrence and diversity is affected both by contact with man and his activities as well as by the existence of animal life.

Impact of environmental factors on aquatic biodiversity in roadside stormwater ponds.

Constructed stormwater ponds mitigate runoff volumes and pollution, and provide other ecosystem services, such as supporting biodiversity, but these services attracted relatively less attention. The impacts of the pollution levels in the water column and sediments, the physical characteristics of ponds, and the presence of amphibians on the macroinvertebrate community composition and biodiversity were explored in twelve stormwater ponds in Norway. Also, the similarities between macroinvertebrate, zooplankton and plant communities were explored. Most of the taxa displayed in the ordination diagram were positively correlated with the pond size and the number of neighbouring ponds, and negatively correlated with the pollution levels in the water column and sediments. However, no statistically significant impacts on the number of taxa and Shannon index were observed. There were low similarities between the macroinvertebrate and zooplankton community compositions as well as between the plant and macroinvertebrate community compositions in the stormwater ponds. We observed a significant positive correlation between the number of plant and of zooplankton taxa, and a weak non-significant positive correlation between the number of plant and of macroinvertebrate taxa. Overall, the explanatory variables had a significant impact on the community composition, but not on the number of taxa nor Shannon index.

Purification of harvested rainwater using slow sand filters with low-cost materials: Bacterial community structure and purifying effect.

Slow sand filters (SSFs) have been shown to effectively improve water quality. The aim of the present study was to obtain low-cost materials (LCMs) as filter mediums (FMs) to efficiently purify harvested rainwater and to document the relationship between bacterial community structure and water purification. The red clay was mixed with crushed limestone and crushed brick, respectively. The mixtures or brick powder were used as the filter media for SSFs. Laboratory column tests were conducted in conjunction with the monitoring of representative water quality parameters (COD, NH4+, CFU and total coliforms) to estimate the performance of low-cost material slow sand filters (LCM-SSFs), including the time needed for biofilm maturation. The relationship between bacterial community structure and SSF performance was determined using a combination of 16S rRNA gene sequencing and an array of statistical techniques. The results demonstrated that LCM-SSFs perform well in purifying harvested rainwater, and are of superior economic benefit. LCMs had a stronger adsorptivity than quartz sand, which enhanced the purification of harvested rainwater before the biofilms matured, and shorten the time required for biofilm maturation. During the 90-day laboratory experiment, a mixture of crushed limestone and red clay exhibited the best performance. The abundance of Opitutae could be used as a potential indicator of NH4+ removal efficiency by SSFs. Schmutzdecke was characterized by abundant, diverse and evenly distributed bacterial communities that produced rich, stable and robust environmental functions, and that possessed an excellent purifying capacity. Environmental conditions associated with low ecological stress, such as neutral pH filter mediums and lucifugal experimental conditions, were conducive to the diversity and evenness of effluent bacterial communities and improved the performance of LCM-SSFs in purifying harvested rainwater.

Evaluation of harvested rainwater quality at primary schools of southwest coastal Bangladesh.

Rainwater is a typical source of drinking water in the coastal areas of Bangladesh given the acute scarcity of drinking water. This study assessed potability of harvested rainwater of primary schools in southwest coastal Bangladesh. Water samples collected from 23 primary schools of Mongla sub-district under Bagerhat district were evaluated for indicator bacteria (total coliform (TC) and E. coli) and physico-chemical parameters (pH, electrical conductivity, turbidity, total dissolved solid, Fe, Zn, Pb, and Cd). Median concentrations of TC and E. coli in the harvested rainwater samples were respectively 3000 cfu/100 ml and 6 cfu/100 ml. However, concentrations of these indicator bacteria were lower at the consumption points which received a prior treatment. Concentration of Pb exceeded the maximum allowable limit for drinking water indicated by WHO and Bangladesh drinking water guideline value in 92% and 61% of the samples respectively, and the mean concentration was 0.08 mg/l (8 times higher than the WHO guideline value). The Pb contamination possibly occurred from the painting on roof railing and roof stair room. Therefore, consumption of harvested rainwater at primary schools may cause substantial health risk for the school-going children.

Factors Influencing Rust (Melampsora apocyni) Intensity on Cultivated and Wild Apocynum venetum in Altay Prefecture, China.

Rust (Melampsora apocyni) on Apocynum venetum is the major constraint to the commercial development of this medicinal herb. To determine the factors influencing rust intensity (maximum disease index [DImax]), rust was investigated from 2011 to 2015 in both cultivated and wild A. venetum plants. Partial least squares path modeling (PLS-PM) was used to analyze the paths and extent of the factors related to pathogen, environment, and host that affect rust intensity. DImax exhibited considerable variations across years and study sites, with variations linked to various factors fostering disease development. PLS-PM explained 80.0 and 70.1% of variations in DImax in cultivated and wild plants, respectively. Precipitation was the key factor determining DImax in both cultivated and wild plants (path coefficient [PC] = 0.313 and 0.544, respectively). In addition, the topsoil water content in cultivated plants and the total vegetation coverage in wild plants were also critical determinants of DImax via their effects on the microclimatic factor (contribution coefficients [CC] = 0.681 and 0.989, respectively; PC = 0.831 and 0.231, respectively). In both cultivated and wild plants, host factors were mainly dominated by A. venetum density (CC = 0.989 and 0.894, respectively), and their effect on DImax via the microclimatic factor (PC = 0.841 and 0.862, respectively) exceeded that via the inoculum factor (PC = 0.705 and 0.130, respectively). However, the indirect effects led to DImax variation, while the dilution effect on host (CC = 0.154) from weed in wild plants led to the indirect effect size in wild plants of 0.200, which was lower than -0.699 in cultivated plants.

[Species diversity of non-tuberculous mycobacteria isolated from aquatic environments of General Pico city, Province of La Pampa (Argentina)]. / Diversidad de especies de micobacterias no tuberculosas aisladas en ambientes acuáticos de la ciudad de General Pico, La Pampa, Argentina.

Non-tuberculous mycobacteria (NTM) are studied not only for their importance as emerging opportunistic pathogens but also for their applications in biotechnology and bioremediation. Our aim was to determine the occurrence and diversity of mycobacteria in different aquatic habitats of General Pico city, Province of La Pampa. The percentage of samples with positive cultures for mycobacteria were the following: 37.5% recovered from the water supply distribution system; 32.6% from the aquifer that supplies water to the distribution system; 36.8% from rain water; 53.1% from the two wetlands in the area of influence; 80% from indoor swimming pools; and 33.3% from water fountains in downtown public squares. Of the 90 NTM isolates, 8.9% could not be identified at the species level with any of the used methods, phenotypic tests and molecular methods. Mycobacterium fortuitum and Mycobacterium gordonae were the most frequently isolated species. Some of the identified species such as, M. fortuitum, M. gordonae, M. intracellulare, M. vaccae, M. lentiflavum and M. nonchromogenicum, have been reported in cases of mycobacteriosis in Argentina. Mycobacteria with values higher than 0.8mg/ml of residual active chlorine were not recovered from the drinking water supply network, whereas in the swimming pools the presence of up to 1.5mg/l was not a constraint. Based on our results, the presence of mycobacteria in aquatic environments is close to 35% and their occurrence and diversity is affected both by contact with man and his activities as well as by the existence of animal life.

Factors affecting microbial and physico-chemical pollutants in stormwater in a typical Chinese urban catchment.

An understanding of microbial pollution characteristics is needed for stormwater reuse and development of microorganism simulations in urban stormwater. This study investigated the discharge characteristics of faecal indicator bacteria (faecal coliforms) in urban runoff by field sampling both the underlying surfaces and the stormwater pipe outlet. Faecal coliform contamination in urban runoff was found to be frequent, and the highest instantaneous concentration reached 2.42 × 106 MPN/100 ml. Faecal coliforms did not show a consistent first flush effect amongst the different surfaces sampled, and this was exacerbated under rainfall events with high intensity. PROMETHEE (Preference Ranking Organization Method for Enrichment Evaluation) and GAIA (Geometrical Analysis for Interactive Aid) analyses were further applied to explore the ranking of pollutants, the relationship among the pollutants, and the factors affecting the contamination in cases of multiple underlying surfaces, multiple pollutants and rainfall events. For the pollutants of suspended solids (SS), total phosphorus (TP) and chemical oxygen demand (COD), the road sample contamination was significantly higher than on the roof surfaces. No such trend in ranking of faecal coliforms was observed. Rainfall depth and intensity were found to have a significant influence on stormwater contamination by physico-chemical pollutants, while having a somewhat smaller influence on faecal coliform contamination. Faecal coliform contamination is closely associated with the index related to the antecedent dry period. The average temperature and average relative humidity also showed a positive relationship with faecal coliform contamination. The effects of antecedent dry period duration on contamination of physico-chemical pollutants and faecal coliform are completely opposite. Antecedent dry period duration was positively related to the contamination of physico-chemical pollutants, but negatively related to faecal coliform contamination. Therefore, three variables, i.e., antecedent dry period duration, average temperature and average relative humidity, might be used to model the survival/die-off of faecal coliform during the antecedent dry period.

Survey of coastal inland pollution sources and their influence on seawater quality in Doam bay, Korea.

Inland pollution sources of Doam bay were investigated from August to October in 2013. A total of 210 sources including rivers, streams, domestic, agricultural and industrial discharge points were identified along the coast, including 32 sources that had outflow. Agricultural sources were the largest inland pollution sources (139, 66.2%). Fecal coliform concentrations were measured. These data were combined with water discharge data to determine daily loads of pollutants discharged from each source into the bay. Fecal coliform concentrations were the highest in domestic discharges. However, they only had slight influence because their discharge volume was small. The most significant pollution source was Tamjin River (St.85) due to large amount of discharge volume. The influence of St.85 reached almost half of Doam bay. Fecal coliform levels of streams increased after rainfall, but decreased overtime. Domestic pollution sources were not affected upon rain event.

Multiple water source use in rural Vanuatu: are households choosing the safest option for drinking?

Households in low- and middle-income countries commonly make use of multiple water sources. However, it remains unclear to what extent their drinking water choices are optimal from a health perspective. This matched cohort study across 10 islands in Vanuatu examined communities with both a groundwater and rainwater source to determine whether their preferred drinking option was the safest in microbial terms. In communities that preferred to drink rainwater, 56.5% of rainwater sources had 'high-risk' or 'very high risk' Escherichia coli contamination (> 10 MPN/100 mL) compared with 26.1% of groundwater sources (p = 0.092). Moreover, a preference for drinking rainwater was significantly associated with rainwater sources having 'high-risk' or 'very high risk' levels of E. coli contamination (p = 0.045). Results show that communities do not necessarily choose the safest water source for drinking. Findings also highlight the need to bolster local capacity to manage water quality risks and for Sustainable Development Goal monitoring to distinguish between protected and unprotected rainwater tanks.

The presence and prevalence of Legionella spp in collected rainwater and its aerosolisation during common gardening activities.

AIMS: To determine the presence and prevalence of Legionella spp in domestic rainwater storage butts and to quantify its aerosolisation when collected rainwater is used for common gardening activities. METHODS: Volunteers were asked to take a water sample from their garden rainwater storage butt. The presence of Legionella was determined using quantitative polymerase chain reaction (qPCR). Two new rainwater storage butts were installed on-site at PHE Porton and positioned in sunlight or in the shade. Ambient conditions and those within the two 'experimental' water butts were continually monitored. A cyclone air sampler was used to detect the presence of Legionella in the air when collected rainwater was poured from a watering can or delivered via a hosepipe attached to a submersible water butt pump. RESULTS: A total of 63 volunteers provided water samples from 113 different rainwater storage butts. Legionella spp was detected in 107 of these samples at a mean concentration of 4.7 × 104 genomic units l-1. Two of these samples also contained L. pneumophila. The water butt positioned in the shade stored water at a significantly lower temperature than that exposed to sunlight. While the concentration of Legionella was significantly higher in this cooler water, meteorological conditions rather than conditions within the water butt had the greatest effect upon Legionella concentration. No Legionella was detected in the air when rainwater was poured from a watering can. However, using a hose pipe on a 'fine spray' setting increased both the number of organisms detected in the air and their dissemination. CONCLUSION: In this study, Legionella spp were common contaminants of collected rainwater. However, the use of rainwater for common gardening activities should not be discouraged. Aerosolisation of Legionella when using a watering can is minimal and any increased risk associated with hose pipe use can be mitigated by using a coarse spray setting.

Impacts of nonpoint source pollutants on microbial community in rain gardens.

Low-impact development (LID) techniques are being applied to reduce non-point source (NPS) pollution which are generated from various land uses. Cost-effective LID design requires consideration of influent runoff properties as well as physical and ecological pollutant-removing mechanisms. However, current LID technology design has failed to reflect the different properties of influent water from various land uses, and the biological design factors in LID facilities causing low efficiency and difficulties in maintenance. This study was conducted to identify biological design factors by analyzing the impact of the pollutants included in influent runoff and physical environment on microbial growth in rain garden facilities applied to different land uses. The results showed that the non-point source pollutant loadings were about 1.5-3 times higher in the runoff from parking lots, which are frequently visited by automobiles than in roof runoff. Type of soil, chemical species, and chemical composition were assessed as internal environmental factors having significant impact on the phylum and the count of microorganisms in the facilities. The growth of Cyanobacteria, Streptophyta, Chlorophyta, Bacillariophyta, and Xanthophyceae was good when there was appropriate water content in the soil, light, and sandy soil. Based on these results, the future design of rain garden facilities should be performed by considering a microorganism appropriate to the properties of the influent pollutants, determining appropriate water content, nutrient content and soil type, and choosing plants that contribute to microbial growth.

A Review of Roof Harvested Rainwater in Australia.

To address concern regarding water sustainability, the Australian Federal Government and many state governments have implemented regulatory mechanisms and incentives to support households to purchase and install rainwater harvesting systems. This has led to an increase in rainwater harvesting in regional and urban Australia. This review examines the implementation of the regulatory mechanisms across Australia. In addition, the literature investigating the potential health consequences of rainwater consumption in Australia was explored. Studies demonstrated that although trace metals such as arsenic, cadmium, chromium, lead, and iron were present in Australian rainwater, these metallic elements were generally found below the health limit guideline, except in high industrial areas. In addition, pathogenic or indicator microorganisms that include, but are not limited to, Escherichia coli, total and faecal coliforms, Campylobacter, Salmonella, Legionella, Pseudomonas, Cryptosporidium, Enterococci, Giardia, Aeromonas, and Mycobacterium avium Complex (MAC) have been detected in rainwater collected in Australia. However, epidemiological evidence suggests that drinking rainwater does not increase the risk of gastrointestinal disease. It was also identified that there is a need for further research investigating the potential for rainwater to be a source of infection for opportunistic pathogens.