RESUMO
Citrobacter freundii is one of the important bacterial diseases responsible for disease outbreaks to wild and cultured fishes globally. However, no known empirical research has focused on exploring relationships between immune response after C. freundii infection in sturgeons. In this study, C. freundii was isolated and identified from artificially breeding Chinese sturgeon, and global measurement of transcriptome response to C. freundii infection in head-kidney and spleen of A. sinensis were conducted to the acknowledgement of the potential mechanisms of pathogen-host interaction triggered by the bacterial infection. In total, differentially expressed genes which significantly associated with immune responses were found to be participated in antigen processing and presentation (MHC I, MHC II, HspA1, Hsp90A, Hsp70, CTSL, and CTSE), and acute phase response (serotransferrin and CP), as well as changing of other immune-related cytokine, such as chemokine and interferon, which proving their reacting and regulatory role during the response of thehost against C. freundii infection in fish. C. freundii can cause serious disease in sturgeon species was first reported in this study, and innate immune responses to C. freundii infection in this study will be conducive to understand the defense mechanisms and making appropriate prevention strategies in A. sinensis aquaculture operations.
Assuntos
Citrobacter freundii/fisiologia , Infecções por Enterobacteriaceae/imunologia , Doenças dos Peixes/imunologia , Proteínas de Peixes/genética , Peixes/imunologia , Rim Cefálico/fisiologia , Baço/fisiologia , Reação de Fase Aguda/genética , Animais , Apresentação de Antígeno/genética , Aquicultura , Quimiocinas/genética , China , Perfilação da Expressão Gênica , Imunidade Inata/genética , Imunomodulação , Interferons/genética , TranscriptomaRESUMO
A 9-week feeding trial was conducted to investigate the effects of graded levels of dietary starch (12%, 17%, 22%, 27% and 32%) on growth, non-specific immune responses, antioxidant capacities, immunity genes expression levels and pathogen resistance in Chinese mitten crab, Eriocheir inensis (initial body weight: 10.5 ± 0.5 g). Results showed that the highest weight gain rate of crabs was obtained in group containing 22% dietary starch. The highest activity of acid phosphatase, phenoloxidase and lysozyme in blood was found in crabs fed with 22-27% dietary starch. Additionally, 17%-27% dietary starch significantly increased the activities of superoxide dismutase and glutathione peroxidase, reduced malondinaldehyde content and then increased the total antioxidant capacities in hepatopancreas of crabs. The highest activity of alanine aminotransferase and aspartate aminotransferase was found in crabs fed with 32% dietary starch, indicating that excess starch had a negative effect on the liver function of crabs. With the dietary starch level increased, the transcription factors gene expression of the pro-inflammatory factors were significantly up-regulated, and the highest ILF2, IL-16, Relish and ADAM10 was found in crabs fed with dietary 32% starch, which may potentially promote the inflammatory response in intestines. Moreover, with the dietary starch increased, the activity of phenoloxidase and lysozyme, as well as the gene expression of crustin, were all increased in crabs after challenge against Citrobacter freundii, which demonstrated that additional dietary starch could provide immune-protection and help crabs improve their resistance against pathogens. In conclusion, these results suggest that adequate dietary starch can increase growth, enhance innate immune responses and promote disease resistance, reduce oxidative stress and inflammatory response in E. inensis. Taken together, 22-27% dietary starch (25.9-30.8% dietary carbohydrate) was suggested as a digestible energy source in crabs feed.
Assuntos
Antioxidantes/metabolismo , Braquiúros/imunologia , Citrobacter freundii/fisiologia , Expressão Gênica/imunologia , Imunidade Inata/efeitos dos fármacos , Intestinos/imunologia , Amido/administração & dosagem , Animais , Braquiúros/efeitos dos fármacos , Relação Dose-Resposta a Droga , Intestinos/efeitos dos fármacosRESUMO
Symbionts are associated with many insects and play several multifunctional roles in insect-microorganism mutualistic relationships. The trichlorphon-degrading symbiont Citrobacter freundii (CF-BD) of the oriental fruit fly Bactrocera dorsalis was recently discovered; however, its intraspecies transmission pathway among flies remains unknown. Here, we use fluorescence in situ hybridization (FISH), PCR detection, and a series of ingenious experiments to reveal that CF-BD was aggregated in rectal pads associated with the female ovipositor, and the CF-BD symbiont was vertically transmitted via egg surface contamination. Although CF-BD was not detected in ovaries, it was found in deposited eggs. In addition, CF-BD was readily acquired horizontally between larvae or adults via oral uptake, although it was not transferred via mating behavior. Surface sterilization of eggs had a negative effect on the insects, which exhibited a lower body weight and a sharp decrease in fecundity, suggesting important biological roles of CF-BD in the fitness of the host insects. Our findings may also help to explain the high pesticide resistance levels of B. dorsalis. Furthermore, identifying a clear transmission pathway of this organophosphorus-degrading symbiont will be useful for pesticide resistance management and future pest control technologies.
Assuntos
Citrobacter freundii/fisiologia , Infecções por Enterobacteriaceae/transmissão , Praguicidas/metabolismo , Simbiose , Tephritidae/microbiologia , Animais , Biotransformação , Citrobacter freundii/metabolismo , Hibridização in Situ Fluorescente , Resistência a Inseticidas , Larva/efeitos dos fármacos , Larva/microbiologia , Reação em Cadeia da Polimerase , Reto/microbiologia , Tephritidae/efeitos dos fármacos , Zigoto/microbiologiaRESUMO
PURPOSE: This study investigated changes in patient nasal and conjunctival flora one year after endoscopic dacryocystorhinostomy (EDSR). METHODS: The prospective study included 20 patients that underwent EDSR due to chronic dacryocystitis. Conjunctival and nasal cultures were obtained one year after EDSR from both study and control groups. Patient characteristics, chronic illnesses, the severity and duration of complaints, culture results, and the stent removal time were recorded and analyzed. RESULTS: In the study group, the most commonly isolated microorganism in the nasal cultures was coagulase-negative staphylococcus (n = 11), and the second most commonly isolated microorganism was Staphylococcus aureus (n = 7). A total of 11 (55%) of the nasal cultures in the study group showed the presence of multi-drug resistant (MDR) bacteria, as did 2 (10%) of the nasal cultures in the control group (p = 0.007). CONCLUSIONS: One year after EDSR surgery with silicon stent placement, we detected changes in the nasal flora in the operated side compared with the non-operated side. Even though more than half of the nasal cultures in the study group were positive for MDR bacteria, these microorganisms did not cause attacks of dacryocystitis or affect surgical success.
Assuntos
Citrobacter freundii/isolamento & purificação , Dacriocistite/cirurgia , Dacriocistorinostomia , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Microbiota , Mucosa Nasal/microbiologia , Pseudomonas aeruginosa/isolamento & purificação , Stents , Idoso , Estudos de Casos e Controles , Doença Crônica , Citrobacter freundii/fisiologia , Farmacorresistência Bacteriana Múltipla/fisiologia , Feminino , Humanos , Masculino , Staphylococcus aureus Resistente à Meticilina/fisiologia , Pessoa de Meia-Idade , Pseudomonas aeruginosa/fisiologia , Silicones , Staphylococcus aureus/isolamento & purificação , Staphylococcus aureus/fisiologiaRESUMO
Beneficial effects of Lactobacilli have been reported, and lactic bacteria are employed for conservation of foods. Therefore, the effects of a Lactobacillus fermentum strain were analyzed regarding inhibitory effects on staphylococci, Candida albicans and enterotoxigenic enterobacteria by transmission electron microscopy (TEM). TEM of bacterial biofilms was performed using cocultures of bacteriocin-producing L. fermentum 97 with different enterotoxigenic strains: Staphylococcus epidermidis expressing the ica gene responsible for biofilm formation, Staphylococcus aureus producing enterotoxin type A, Citrobacter freundii, Enterobacter cloaceae, Klebsiella oxytoca, Proteus mirabilis producing thermolabile and thermostable enterotoxins determined by elt or est genes, and Candida albicans. L. fermentum 97 changed morphological features and suppressed biofilm formation of staphylococci, enterotoxigenic enterobacteria and Candida albicans; a marked transition to resting states, a degradation of the cell walls and cytoplasm, and a disruption of mature bacterial biofilms were observed, the latter indicating efficiency even in the phase of higher cell density.
Assuntos
Antibiose , Fenômenos Fisiológicos Bacterianos , Biofilmes , Candida albicans/fisiologia , Limosilactobacillus fermentum/fisiologia , Staphylococcus aureus/fisiologia , Bactérias/crescimento & desenvolvimento , Bacteriocinas/metabolismo , Candida albicans/crescimento & desenvolvimento , Parede Celular/metabolismo , Citrobacter freundii/crescimento & desenvolvimento , Citrobacter freundii/fisiologia , Enterobacteriaceae/crescimento & desenvolvimento , Enterobacteriaceae/fisiologia , Microscopia Eletrônica de Transmissão , Proteus mirabilis/crescimento & desenvolvimento , Proteus mirabilis/fisiologia , Staphylococcus aureus/crescimento & desenvolvimentoRESUMO
The type VI secretion system (T6SS) as a virulence factor-releasing system contributes to virulence development of various pathogens and is often activated upon contact with target cells. Citrobacter freundii strain CF74 has a complete T6SS genomic island (GI) that contains clpV, hcp-2, and vgr T6SS genes. We constructed clpV, hcp-2, vgr, and T6SS GI deletion mutants in CF74 and analyzed their effects on the transcriptome overall and, specifically, on the flagellar system at the levels of transcription and translation. Deletion of the T6SS GI affected the transcription of 84 genes, with 15 and 69 genes exhibiting higher and lower levels of transcription, respectively. Members of the cell motility class of downregulated genes of the CF74ΔT6SS mutant were mainly flagellar genes, including effector proteins, chaperones, and regulators. Moreover, the production and secretion of FliC were also decreased in clpV, hcp-2, vgr, or T6SS GI deletion mutants in CF74 and were restored upon complementation. In swimming motility assays, the mutant strains were found to be less motile than the wild type, and motility was restored by complementation. The mutant strains were defective in adhesion to HEp-2 cells and were restored partially upon complementation. Further, the CF74ΔT6SS, CF74ΔclpV, and CF74Δhcp-2 mutants induced lower cytotoxicity to HEp-2 cells than the wild type. These results suggested that the T6SS GI in CF74 regulates the flagellar system, enhances motility, is involved in adherence to host cells, and induces cytotoxicity to host cells. Thus, the T6SS plays a wide-ranging role in C. freundii.
Assuntos
Aderência Bacteriana , Sistemas de Secreção Bacterianos , Toxinas Bacterianas/metabolismo , Citrobacter freundii/fisiologia , Flagelos/fisiologia , Regulação Bacteriana da Expressão Gênica , Locomoção , Citrobacter freundii/genética , Flagelina/biossíntese , Deleção de Genes , Perfilação da Expressão Gênica , Redes Reguladoras de Genes , Teste de Complementação Genética , Ilhas Genômicas , Células Hep G2 , Hepatócitos/microbiologia , Humanos , Biossíntese de Proteínas , Transcrição Gênica , Fatores de Virulência/metabolismoRESUMO
Various studies have shown that cold plasma is capable of inactivating microorganisms located on a variety of food surfaces, food packaging materials and process equipment under atmospheric pressure conditions; however, less attention has been paid to the impact of cold plasma on microorganisms in liquid foodstuffs. The present study investigates cold plasma's ability to inactivate Citrobacter freundii in apple juice. Optical emission spectroscopy (OES) and temperature measurements were performed to characterise the plasma source. The plasma-related impact on microbial loads was evaluated by traditional plate count methods, while morphological changes were determined using scanning electron microscopy (SEM). Physiological property changes were obtained through flow cytometric measurements (membrane integrity, esterase activity and membrane potential). In addition, mathematical modelling was performed in order to achieve a reliable prediction of microbial inactivation and to establish the basis for possible industrial implementation. C. freundii loads in apple juice were reduced by about 5 log cycles after a plasma exposure of 480s using argon and 0.1% oxygen plus a subsequent storage time of 24h. The results indicate that a direct contact between bacterial cells and plasma is not necessary for achieving successful inactivation. The plasma-generated compounds in the liquid, such as H2O2 and most likely hydroperoxy radicals, are particularly responsible for microbial inactivation.
Assuntos
Bebidas/microbiologia , Citrobacter freundii/efeitos dos fármacos , Microbiologia de Alimentos , Conservação de Alimentos/métodos , Malus/microbiologia , Viabilidade Microbiana , Gases em Plasma/farmacologia , Argônio/farmacologia , Citrobacter freundii/fisiologia , Contagem de Colônia Microbiana , Citometria de Fluxo , Peróxido de Hidrogênio/farmacologia , Cinética , Modelos Biológicos , Oxigênio/farmacologia , Temperatura , Fatores de TempoRESUMO
Skin is an important innate immune organ in fish; however, little is known about the skin's immune response to infectious pathogens. We conducted a comparative analysis of the acute immune response of Zebrafish Danio rerio skin against gram-positive (Staphylococcus chromogenes) and gram-negative (Citrobacter freundii) bacterial infections. Gene expression profiles induced from the two different infections were identified by microarray hybridization, with many genes demonstrating an acute immune response in the skin. Differentially expressed genes were mainly involved in response to stress and stimulus, complement activation, acute-phase response, and defense and immune response. Compared with transcription patterns of skin from the two infections, a similar innate immunity (e.g., transferrin, coagulation factor, complements, and lectins) was observed but with different acute-phase genes (e.g., ceruloplasmin, alpha-1-microglobulin, vitellogenin, and heat shock protein). These results suggest that the skin of fish plays an important role in the innate immune responses to bacterial infection.
Assuntos
Citrobacter freundii/fisiologia , Infecções por Enterobacteriaceae/veterinária , Dermatopatias Bacterianas/veterinária , Staphylococcus/fisiologia , Peixe-Zebra , Animais , Infecções por Enterobacteriaceae/microbiologia , Infecções por Enterobacteriaceae/patologia , Dermatopatias Bacterianas/microbiologia , Dermatopatias Bacterianas/patologiaRESUMO
BACKGROUND: Diffusely adherent Escherichia coli (DAEC) have been considered a diarrheagenic category of E. coli for which several potential virulence factors have been described in the last few years. Despite this, epidemiological studies involving DAEC have shown inconsistent results. In this work, two different collections of DAEC possessing Afa/Dr genes, from children and adults, were studied regarding characteristics potentially associated to virulence. RESULTS: DAEC strains were recovered in similar frequencies from diarrheic and asymptomatic children, and more frequently from adults with diarrhea (P < 0.01) than from asymptomatic adults. Association with diarrhea (P < 0.05) was found for SAT-positive strains recovered from children and for curli-positive strains recovered from adults. Mixed biofilms involving DAEC and a Citrobacter freundii strain have shown an improved ability to form biofilms in relation to the monocultures. Control strains have shown a greater diversity of Afa/Dr adhesins and higher frequencies of cellulose, TTSS, biofilm formation and induction of IL-8 secretion than strains from cases of diarrhea in children. CONCLUSIONS: DAEC strains possessing Afa/Dr genes isolated from children and adults represent two different bacterial populations. DAEC strains carrying genes associated to virulence can be found as part of the normal microbiota present in asymptomatic children.
Assuntos
Diarreia/microbiologia , Infecções por Escherichia coli/microbiologia , Escherichia coli/classificação , Escherichia coli/isolamento & purificação , Fatores de Virulência/genética , Adulto , Biofilmes/crescimento & desenvolvimento , Pré-Escolar , Citrobacter freundii/fisiologia , Escherichia coli/patogenicidade , Escherichia coli/fisiologia , Genótipo , Humanos , LactenteRESUMO
Recent trials have questioned the safety of untargeted oral iron supplementation in developing regions. Excess of luminal iron could select for enteric pathogens at the expense of beneficial commensals in the human gut microflora, thereby increasing the incidence of infectious diseases. The objective of the current study was to determine the effect of high iron availability on virulence traits of prevalent enteric pathogens at the host-microbe interface. A panel of enteric bacteria was cultured under iron-limiting conditions and in the presence of increasing concentrations of ferric citrate to assess the effect on bacterial growth, epithelial adhesion, invasion, translocation and epithelial damage in vitro. Translocation and epithelial integrity experiments were performed using a transwell system in which Caco-2 cells were allowed to differentiate to a tight epithelial monolayer mimicking the intestinal epithelial barrier. Growth of Salmonella typhimurium and other enteric pathogens was increased in response to iron. Adhesion of S. typhimurium to epithelial cells markedly increased when these bacteria were pre-incubated with increasing iron concentration (Pâ=â0.0001), whereas this was not the case for the non-pathogenic Lactobacillus plantarum (Pâ=â0.42). Cellular invasion and epithelial translocation of S. typhimurium followed the trend of increased adhesion. Epithelial damage was increased upon incubation with S. typhimurium or Citrobacter freundii that were pre-incubated under iron-rich conditions. In conclusion, our data fit with the consensus that oral iron supplementation is not without risk as iron could, in addition to inducing pathogenic overgrowth, also increase the virulence of prevalent enteric pathogens.
Assuntos
Mucosa Intestinal/metabolismo , Ferro/metabolismo , Salmonella typhimurium/crescimento & desenvolvimento , Aderência Bacteriana/efeitos dos fármacos , Translocação Bacteriana/efeitos dos fármacos , Disponibilidade Biológica , Células CACO-2 , Citrobacter freundii/crescimento & desenvolvimento , Citrobacter freundii/fisiologia , Suplementos Nutricionais , Relação Dose-Resposta a Droga , Enterococcus faecalis/crescimento & desenvolvimento , Enterococcus faecalis/fisiologia , Epitélio/metabolismo , Epitélio/microbiologia , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/fisiologia , Compostos Férricos/farmacologia , Interações Hospedeiro-Patógeno , Humanos , Mucosa Intestinal/microbiologia , Intestinos/microbiologia , Ferro da Dieta/administração & dosagem , Ferro da Dieta/metabolismo , Ferro da Dieta/farmacocinética , Lactobacillus plantarum/crescimento & desenvolvimento , Lactobacillus plantarum/fisiologia , Salmonella typhimurium/fisiologiaRESUMO
The rapidly growing biodiesel industry has created a scenario, where it is both important and challenging to deal with the enormous amount of crude glycerol generated as an inherent by-product. With every 100 gallons of biodiesel produced, 5-10 gallons of the crude glycerol is left behind containing several impurities which makes its disposal difficult. The objective of the present investigation was to evaluate the impact of biodiesel-derived crude glycerol upon microbial growth and production of 1,3-propanediol by Citrobacter freundii. Five different grades of crude glycerol (obtained from biodiesel preparation using jatropha, soybean, sunflower, rice bran and linseed oils) were used. Crude glycerol caused significant inhibition of microbial growth and subsequently 1,3-propanediol production as compared to pure glycerol. Therefore, a process was developed for the treatment of crude glycerol using solvents before fermentation wherein four different non-polar solvents were examined yielding different grades of pretreated glycerol. Subsequently, the potential toxic effects of pretreated glycerol on the growth and 1,3-propanediol production by C. freundii was evaluated. In case of petroleum ether-treated crude glycerol obtained from jatropha & linseed and hexane-treated crude glycerol obtained from rice bran, the yields obtained were comparable to the pure glycerol. Similarly, soybean-derived glycerol gave comparable results after treatment with either hexane or petroleum ether.
Assuntos
Biocombustíveis/microbiologia , Citrobacter freundii/fisiologia , Glicerol/isolamento & purificação , Glicerol/metabolismo , Propilenoglicóis/metabolismo , Solventes/química , Proliferação de Células , Propilenoglicóis/isolamento & purificaçãoRESUMO
Bacterial swarming motility is a flagella-dependent translocation on the surface environment. It has received extensive attention as a population behavior involving numerous genes. Here, we report that Citrobacter freundii, an opportunistic pathogen, exhibits swarming movement on a solid medium surface with appropriate agar concentration. The swarming behavior of C. freundii was described in detail. Insertional mutagenesis with transposon Mini-Tn5 was carried out to discover genetic determinants related to the swarming of C. freundii. A number of swarming genes were identified, among which flhD, motA, motB, wzx, rfaL, rfaJ, rfbX, rfaG, rcsD, rcsC, gshB, fabF, dam, pgi, and rssB have been characterized previously in other species. In mutants related to lipopolysaccharide synthesis and RcsCDB signal system, a propensity to form poorly motile bacterial aggregates on the agar surface was observed. The aggregates hampered bacterial surface migration. In several mutants, the insertion sites were identified to be in the ORF of yqhC, yeeZ, CKO_03941, glgC, and ttrA, which have never been shown to be involved in swarming. Our results revealed several novel characteristics of swarming motility in C. freundii which are worthy of further study.
Assuntos
Citrobacter freundii/fisiologia , Proteínas de Bactérias/genética , Proteínas de Bactérias/metabolismo , Western Blotting , Citrobacter freundii/genética , Citrobacter freundii/ultraestrutura , Microscopia Eletrônica de Transmissão , Fases de Leitura Aberta/genéticaRESUMO
BACKGROUND: Enteroaggregative Escherichia coli (EAEC) are enteropathogenic strains identified by the aggregative adhesion (AA) pattern that share the capability to form biofilms. Citrobacter freundii is classically considered as an indigenous intestinal species that is sporadically associated with diarrhea. RESULTS: During an epidemiologic study focusing on infantile diarrhea, aggregative C. freundii (EACF) and EAEC strains were concomitantly recovered from a severe case of mucous diarrhea. Thereby, the occurrence of synergic events involving these strains was investigated. Coinfection of HeLa cells with EACF and EAEC strains showed an 8-fold increase in the overall bacterial adhesion compared with single infections (P < 0.001). The synergic effect was mediated by physical interactions among the bacteria and primed in the absence of chemical signaling and without the participation of host cells. Thus, significant increases (2.7-fold on average) in bacterial adhesion were also observed during the formation of mixed biofilms on abiotic surfaces. Bacterial settling assays showed that EAEC strains harboring F-pili genes (traA) were capable of forming bacterial aggregates only in the presence of EACF. Scanning electronic microscopy analyses revealed that bacterial aggregates as well as enhanced biofilms formed by EACF and traA-positive EAEC were mediated by non-bundle forming, flexible pili. Moreover, mixed biofilms formed by EACF and traA-positive EAEC strains were significantly reduced using nonlethal concentration of zinc, a specific inhibitor of F pili. In addition, EAEC strains isolated from diarrheic children frequently produced single biofilms sensitive to zinc. CONCLUSIONS: Putative F pili expressed by EAEC strains boosted mixed biofilm formation when in the presence of aggregative C. freundii.
Assuntos
Biofilmes/crescimento & desenvolvimento , Citrobacter freundii/fisiologia , Diarreia/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Escherichia coli Enteropatogênica/fisiologia , Proteínas de Escherichia coli/fisiologia , Proteínas de Fímbrias/fisiologia , Adesinas de Escherichia coli/genética , Antibacterianos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Aderência Bacteriana/genética , Biofilmes/efeitos dos fármacos , Citrobacter freundii/patogenicidade , Citrobacter freundii/ultraestrutura , Escherichia coli Enteropatogênica/genética , Escherichia coli Enteropatogênica/patogenicidade , Escherichia coli Enteropatogênica/ultraestrutura , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteínas de Fímbrias/genética , Proteínas de Fímbrias/metabolismo , Vidro , Células HeLa , Humanos , Lactente , Microscopia Eletrônica de Varredura , Percepção de Quorum , Zinco/farmacologiaRESUMO
AIM: To study variability of enzymatic apparatus of opportunistic enterobacteria. MATERIALS AND METHODS: Clinical strains of Morganella morganii, Citrobacter freundii, Proteus mirabilis isolated from patients treated in Irkutsk Regional Hospital for Infectious Diseases. Activity of cellulase and lipase as well as amount of auxins and gibberellins was studied in these bacteria at different cultivation temperatures. RESULTS: It was shown that studied species isolated from humans enterobacteria are able to produce plant growth regulators amount of which depends from cultivation temperature and type of microorganism. Activity of cellulase sharply rises if temperature falls. CONCLUSION: Obtained results show high adaptation potential of opportunistic bacteria from Enterobacteriaceae family. Switch on saprophytic mechanism after fall of temperature to environment-corresponding values allows them to survive in soil and arrange different interactions with soil biota including plants.
Assuntos
Adaptação Fisiológica , Citrobacter freundii/fisiologia , Morganella morganii/fisiologia , Proteus mirabilis/fisiologia , Celulase/biossíntese , Citrobacter freundii/metabolismo , Citrobacter freundii/patogenicidade , Giberelinas/metabolismo , Humanos , Ácidos Indolacéticos/metabolismo , Lipase/metabolismo , Morganella morganii/metabolismo , Morganella morganii/patogenicidade , Proteus mirabilis/metabolismo , Proteus mirabilis/patogenicidade , TemperaturaRESUMO
AIMS: To test the effects of C : N : P ratio modification of a well-known nutrient medium formulation, the Endo formulation on biofilm formation by Enterobacter cloacae Ecl and Citrobacter freundii Cf1 in both single-species and binary species biofilms. METHODS AND RESULTS: The C : N : P atom : atom ratio of a well-known nutrient medium formulation, the Endo formulation, that has been applied in fermentative biohydrogen studies, was modified to include two different C concentrations, one containing 17.65 g l(-1) and the other 8.84 g l(-1) sucrose, each containing four different C : N : P ratios, two at higher C : N : P ratios (334 : 84 : 16.8 and 334 : 84 : 3) and two at lower C : N : P ratios (334 : 28 : 5.6 and 334 : 28 : 1). Attached cells were enumerated after dislodging the biofilms that had formed on granular activated carbon (GAC). The modified medium containing 17.65 g l(-1) sucrose and having a C : N : P ratio of 334 : 28 : 5.6 resulted in significantly (P < 0.05) higher counts of attached cells for both single-species biofilms at 7.73 log(10) CFU g(-1) GAC and 9.3 log(10)CFU g(-1) GAC for Ent. cloacae Ecl and Cit. freundii Cf1, respectively, and binary species biofilms at 8.2 log(10) CFU g(-1) GAC and 6.34 log(10) CFU g(-1) GAC for Ent. cloacae Ecl and Cit. freundii Cf1, respectively. Scanning electron micrographs showed qualitative evidence that the 334 : 28 : 5.6 ratio encouraged more complex and extensive biofilm growth for both single-species and binary species biofilms. CONCLUSIONS: The differences in the attachment numbers between the different ratios were found not to be a result of the individual actions of the bacterial isolates involved but rather because of the effects of the various C : N : P ratios. The 334 : 28 : 5.6 ratio showed significantly (P < 0.05) higher counts of attached cells for both single-species and binary species biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY: This study indicates that C : N : P ratios should be a key consideration with regard to maximizing biofilm formation in shake flask and fluidized bed bioreactor studies as well as understanding fundamental factors affecting biofilm growth in natural environments.
Assuntos
Biofilmes/efeitos dos fármacos , Citrobacter freundii/fisiologia , Meios de Cultura/química , Enterobacter cloacae/fisiologia , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/crescimento & desenvolvimento , Reatores Biológicos , Carbono/farmacologia , Citrobacter freundii/efeitos dos fármacos , Citrobacter freundii/crescimento & desenvolvimento , Citrobacter freundii/ultraestrutura , Meios de Cultura/farmacologia , Relação Dose-Resposta a Droga , Enterobacter cloacae/efeitos dos fármacos , Enterobacter cloacae/crescimento & desenvolvimento , Enterobacter cloacae/ultraestrutura , Microscopia Eletrônica de Varredura , Nitrogênio/farmacologia , Fósforo/farmacologia , Sacarose/farmacologiaRESUMO
Stable flies, Stomoxys calcitrans (L.), are the most important pests of cattle in the United States. However, adequate management strategies for stable flies, especially for pastured cattle, are lacking. Microbial/symbiont-based approaches offer novel venues for management of insect pests and/or vector-borne human and animal pathogens. Unfortunately, the fundamental knowledge of stable fly-microbial associations and their effect on stable fly biology is lacking. In this study, stable flies laid greater numbers of eggs on a substrate with an active microbial community (> 95% of total eggs oviposited) than on a sterilized substrate. In addition, stable fly larvae could not develop in a sterilized natural or artificial substrate/medium. Bacteria were isolated and identified from a natural stable fly oviposition/developmental habitat and their individual effect on stable fly oviposition response and larval development was evaluated in laboratory bioassays. Of nine bacterial strains evaluated in the oviposition bioassays, Citrobacter freundii stimulated oviposition to the greatest extent. C. freundii also sustained stable fly development, but to a lesser degree than Serratia fanticola. Serratia marcescens and Aeromonas spp. neither stimulated oviposition nor supported stable fly development. These results demonstrate a stable fly bacterial symbiosis; stable fly larval development depends on a live microbial community in the natural habitat, and stable fly females are capable of selecting an oviposition site based on the microbially derived stimuli that indicate the suitability of the substrate for larval development. This study shows a promising starting point for exploiting stable fly-bacterial associations for development of novel approaches for stable fly management.
Assuntos
Fenômenos Fisiológicos Bacterianos , Muscidae/microbiologia , Muscidae/fisiologia , Oviposição/fisiologia , Simbiose/fisiologia , Análise de Variância , Animais , Bactérias/classificação , Bactérias/isolamento & purificação , Bioensaio/veterinária , Peso Corporal , Citrobacter freundii/isolamento & purificação , Citrobacter freundii/fisiologia , Feminino , Larva/crescimento & desenvolvimento , Larva/microbiologia , Muscidae/crescimento & desenvolvimento , Pupa/crescimento & desenvolvimento , Pupa/microbiologia , RNA Ribossômico 16S/genética , Serratia/isolamento & purificação , Serratia/fisiologia , Comportamento Sexual Animal/fisiologia , Esterilização , Fatores de TempoRESUMO
Data of the ultrastructural cellular changes of conditionally pathogenic enterobacteria, including K. pneumoniae, C. freundii and P. mirabilis cells impacts to bacteriocin-producing L. acidophilus are presented. Enterobacteria in response to the bacteriocinogenic effect of lactobacilli are manifestated by expressive destructions of sensitive to pore formation bacteriocin cells. Various morphological types of enterobacteria cells with increase of involution, lysing and resting forms are revealed. The specific ultrastructural changes of enterobacteria cells which evidencing the significant destructive processes of the cells membranes are detected. The destabilization of cellular wall in expansion periplasmic spaces and appearance of the ultrastructural reorganization of bacterial cells nucleoid also are registrated. Revealing the mechanism of lactobacilli secreted bacteriocin action to conditionally pathogenic enterobacteria might provide new ways to select the effective highly antagonistic probiotic strains.
Assuntos
Citrobacter freundii/fisiologia , Klebsiella pneumoniae/fisiologia , Lactobacillus acidophilus/fisiologia , Proteus mirabilis/fisiologia , Antibiose , Bacteriocinas/metabolismo , Membrana Celular/patologia , Membrana Celular/ultraestrutura , Parede Celular/patologia , Parede Celular/ultraestrutura , Citrobacter freundii/ultraestrutura , Klebsiella pneumoniae/ultraestrutura , Microscopia Eletrônica , Proteus mirabilis/ultraestruturaRESUMO
Enrichment of acrylonitrile (AN) degrading bacterial culture from contaminated soil resulted in the isolation of two cultures which were identified as gram negative small rods (C1) and gram positive cocci (C2). One of the cultures (C1) was identified as Citrobacter fruendii on the basis of biochemical and physiological tests. Both the cultures (C1 and C2) were able to utilize acrylonitrile up to a concentration of 2000 mg/l as the sole source of carbon and nitrogen. The studies also confirmed that the acrylonitrile contaminated soil when ploughed with well mixed AN degrading culture, diammonium phosphate and farmyard manure, could be completely remediated within two months from the date of soil amendment.
Assuntos
Acrilonitrila/metabolismo , Carcinógenos/metabolismo , Microbiologia do Solo , Poluentes do Solo/metabolismo , Agricultura , Animais , Biodegradação Ambiental , Citrobacter freundii/fisiologia , EstercoRESUMO
The effects of adhesion and of biofilm development on the efficiency of cleaning and disinfection procedures were investigated on three strains belonging to the E. coli, C. freundii and K. pneumoniae species, which were able to raise more or less complex biofilms. Resistance to a rinsing procedure was strain dependent but not related to the biofilm structure: E. coli was poorly adherent although embedded in an organic matrix. Conversely, a similar increase in the heat- and disinfectant-resistance was observed, regardless of the complexity of the biofilm (more or less significant organic matrix). These result suggested the essential role of the bacterial physiological state in resistance to sanitation procedures.
Assuntos
Aderência Bacteriana , Citrobacter freundii/fisiologia , Desinfecção/métodos , Escherichia coli/fisiologia , Klebsiella pneumoniae/fisiologia , Leite/microbiologia , Saneamento , Animais , Biofilmes , Adesão CelularRESUMO
Enteropathogenic Escherichia coli (EPEC) is a causative agent of diarrhoea in humans. Localized adherence of EPEC onto intestinal mucosa was reproduced in an in vitro adherence assay with cultured human epithelial cells. We found that the efficiency of EPEC adherence to a mouse-derived colonic epithelial cell line, CMT-93, was remarkably lower than its adherence to human-derived intestinal cell lines, such as Intestine-407 or Caco-2. Although EPEC did adhere to some cell lines derived from non-human species, fixing the cells with formalin to inactivate one or more formalin-sensitive factors allowed us to observe species-specific differences in EPEC adherence. In contrast to these results, an EPEC mutant that is defective in bundle-forming pili (BFP) production adhered as efficiently to CMT-93 cells as to Caco-2 cells. Furthermore, Citrobacter rodentium expressing BFP adhered to Caco-2 cells much more efficiently than to CMT-93 cells. Finally, a purified BfpA-His6 fusion protein showed higher affinity for Caco-2 cells than for CMT-93 cells, and inhibited EPEC adherence. Following BFP-mediated adherence, secretion of EspB from adherent bacteria and reorganization of F-actin in the host cells was observed. EPEC adhering to CMT-93 cells induced far less secretion of EspB, or reorganization of F-actin in the host CMT-93 cells, than did EPEC adhering to Caco-2 cells. These results indicated that BFP plays an important role in the cell-type-dependent adherence of EPEC and in the progression to the later steps in EPEC adherence.